CN103361270A - Multi-field coupled in-vitro cell experimental device and method - Google Patents
Multi-field coupled in-vitro cell experimental device and method Download PDFInfo
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- CN103361270A CN103361270A CN2013103041901A CN201310304190A CN103361270A CN 103361270 A CN103361270 A CN 103361270A CN 2013103041901 A CN2013103041901 A CN 2013103041901A CN 201310304190 A CN201310304190 A CN 201310304190A CN 103361270 A CN103361270 A CN 103361270A
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Abstract
The invention discloses a multi-field coupled in-vitro cell experimental device and a method. The device includes a container internally equipped with a thermal insulation interlayer and a magnetic stirrer, a flange cover provided with a plurality of holes on the surface, and a multifunctional sample stand. The upper end and the lower end of the container are respectively provided with a water inlet and a water outlet. The water outlet is provided with a ball valve. The magnetic stirrer is used for balancing the water bath temperature. The flange cover is connected to the top of the container to form a structure able to open or close the container. A porous cell culture plate-carrying electromagnetic field generation device and/or a single porous cell culture plate can be placed on the sample stand, which is placed in the container during experiment. The method comprises: putting the porous cell culture plate-carrying electromagnetic field generation device on the sample stand, then placing the sample stand into the container, and controlling the atmosphere, temperature and humidity conditions required by cell culture. The multi-field coupled in-vitro cell experimental device provided in the invention has the advantages of simple structure, low cost, convenient operation and multiple functions, and can meet the need of performing multiple groups of experiments at the same time.
Description
Technical field
The present invention relates to a kind of cell culture apparatus and method, especially a kind of many coupling In vitro cell experiment device and methods belong to medical instruments field.
Background technology
Natural somatocyte is in the complicated body microenvironment, and the effect of the stress-strain of natural body, bio-electric field, biological magnetic field affects the structure of body tissue growth, the development of morphology and function.From nineteen fifty-seven Fukada and Yasuda the discovery of the macroforce electrical property of bone and subsequently Bassett discovery stress are promoted that the reason of bone reconstruction may be the current potential that stress produces in bone, stimulated cell response, induced the physiological change of cell.
Biomechanics has in clinical application: the limbs lengthening art of orthopaedics, the pressurization ligamentopexis of fracture, cranium Jaw distraction art and skin soft tissue stretching art etc.The action of a magnetic field aspect, there is animal experiment to find: under the action of a magnetic field, bone trabecula number and the thickness of experimental group all increase, the maximum tensile strength of femur and the maximum compressive strength of centrum all reach normally, and the bone surface such as human spine, femur and inside will produce time dependent induced current.By electro-magnetic biological effect, affect the osteocyte metabolism, improve bone density, improve bone mass, reach the purpose of prevention and treatment osteoporosis and fracture.Bioelectrical effect is the moulding of bone and rebuild important role, adopts electricity irritation to promote that union of fracture is orthopaedics methods for the treatment of commonly used.Apply the formed charge accumulated of negative voltage at fracture and be conducive to union of fracture.A large amount of experimentation on animalies also prove, continuous direct current, and pulse direct current, continuous intravenous electric field and pulse electromagnetic field all can promote growth and the reparation of bone, and widespread use in clinical treatment.
Utilize the clinical treatment method of Biological Strength, electricity, magnetic field effect to be widely used, yet its Molecular Biology Mechanism is not set forth yet fully.To the in vitro study of active somatic cell under power, electricity, magnetic field environment to inquire in it the Molecular Biology Mechanism significant.The power that the research of In vitro cell experiment is set except structure, electricity, the magnetic field, its experimental situation must adapt with the cell culture environment of routine, otherwise can not accurately simulate the living organism internal milieu, causes its result of study to lack scientific basis.
At present, cell is applied the experimental installation that the field of force, magnetic field and electric field study four-point bending cell augmenter, pore pressurizing cell cultivation instrument, cell in vitro loading device with static magnetic field and cell electrophoresis apparatus etc., yet these equipment and instruments exist that application function is single, complex operation, price are high, can not independently carry out cell cultures, be difficult to realize that many group experiments carry out, are unfavorable for studying the multiple not convenient factors such as experimental study of a large amount of samples of these type of needs of biomaterial and cell response simultaneously.
Summary of the invention
The objective of the invention is in order to solve the defective of above-mentioned prior art, a kind of simple in structure, with low cost, easy to operate, diverse in function is provided, and can satisfy many coupling In vitro cell experiment devices that carry out simultaneously many group experiments.
Another object of the present invention is to provide a kind of many coupling In vitro cell experiment methods of said apparatus.
Purpose of the present invention can reach by taking following technical scheme:
Many coupling In vitro cell experiment devices is characterized in that: but comprise the container of constant control water temperature, be complementary with container and the surface be provided with a plurality of perforates blind flange, can realize the uniform magnetic stirring apparatus of distribution of water temperature and can place sample pallet with the electromagnetic field generator of porous cell culture plate and/or single porous cell culture plate; The top and bottom of described container are respectively arranged with filler and dewatering outlet, and place, described dewatering outlet is provided with ball valve, but described blind flange is connected to the structure that the opened/closed container is formed on the top of container, and described magnetic stirring apparatus is installed in the bottom of container; Described sample pallet places in the container when experiment.
As a kind of preferred version, described container lower end also is provided with heating tube and be used for places the first thermometer hole of the first thermometer, described heating tube be connected thermometer and be connected respectively realization constant control water temperature with outside temperature regulating device.
As a kind of preferred version, the perforate that described blind flange surface arranges comprises air inlet port, production well, circuit jack, is used for placing barometric weather gage hole, is used for placing the second thermometer hole of the second thermometer and is used for placing hygroscopic wet bulb thermometer hole.
As a kind of preferred version, described electromagnetic field generator comprises one group of parallel Helmholtz coil, one group of parallel-plate electrode, dull and stereotyped pallet and power supply, and described parallel-plate electrode is placed on the dull and stereotyped pallet, and described power supply bottom is provided with terminal stud; In the time of in electromagnetic field generator places container, the wiring of described terminal stud is connected with the external power source control device by the circuit jack.
As a kind of preferred version, described sample pallet comprises Multilayer Samples platform and at least three thread racks that are used for supporting sample table, described every layer of sample table is the irregular roundness shape that crops the part arc length, its surface is provided with at least three bracket holes that are complementary with thread rack, and described thread rack passes behind the bracket holes to be regulated by hex-nut and the height of fixed sample platform.
As a kind of preferred version, the surface of described every layer of sample table also is provided with a plurality of borings for placing centrifuge tube.
As a kind of preferred version, described container is the cylindrical straight tubular that insulating interlayer is housed, and conveniently the sample pallet is put into or is proposed, and improve the homogeneity of internal temperature, and it adopts stainless material to make, and can stablize pressurize.
As a kind of preferred version, be provided with rubber cradle between the top of described blind flange and container, to improve the sealing effectiveness of container.
Another object of the present invention can reach by taking following technical scheme:
Many coupling In vitro cell experiment methods is characterized in that may further comprise the steps:
1) with after the container sterilization sterilization, in container, add a certain amount of deionized water to keep the humidity environment of cell cultures:
The porous cell culture plate that 2) will be placed with cell sample is placed between the parallel-plate electrode of electromagnetic field generator;
3) electromagnetic field generator is placed on the sample pallet, then the sample pallet is placed in the container, the circuit jack is passed through in the wiring of terminal stud be connected with the external power source control device, by the blind flange unopened container;
4) with air inlet port with the 5%CO that contains of rate of flow meter
2Medical mixed gas gas-holder link to each other, then adjust pore, monitor by weather gage, obtain stable air pressure;
5) closed ball valve is closed the dewatering outlet, makes container be in air-tight state, injects an amount of water in the filler, opens outside temperature regulating device, utilizes the heating tube heating, monitors by the first thermometer, makes water temperature constant in preset temp A;
6) after outside temperature regulating device is opened for some time, be actual temperature B by gas temperature in the second thermometer measuring vessel, by B=37 ℃ of magnetic stirring apparatus control, in the filler, inject the interior humidity of a small amount of water management container again, read registration by wet bulb thermometer;
7) after the end, turn off first external power source control device, outside temperature regulating device and gas-holder pressurizing valve, the re-adjustment production well reduces the container internal gas pressure, opens blind flange and takes out the sample pallet.
As a kind of preferred version, step 3) described when being placed on electromagnetic field generator on the sample pallet, simultaneously the single porous cell culture plate that is placed with cell sample is placed on the sample pallet.
The present invention has following beneficial effect with respect to prior art:
1, many coupling In vitro cell experiment devices of the present invention can be realized many couplings of the field of force, magnetic field, electric field by electromagnetic field generator, also can realize the independent use of the field of force, magnetic field, electric field, can conveniently study more real biomechanics, bioelectromagnetics at the Discussion of Mechanism of molecular biology level.
2, many coupling In vitro cell experiment devices of the present invention can be controlled the required atmosphere of cell cultures, temperature and humidity condition in container, the magnetic stirring apparatus that install the bottom, can realize being evenly distributed of water temperature, the spatial distribution that has guaranteed the internal tank experimental temperature is even, can independently carry out In vitro cell experiment, need not to put into specific cell culture incubator.
3, many coupling In vitro cell experiment devices of the present invention are when experiment, the wiring of electromagnetic field generator can be connected with the external power source control device by the circuit jack, according to actual needs, utilize the external power source control device, can realize the cell culture environment under stationary magnetic field, pulsed magnetic field, steady electric field, the constant pressure, need the exert oneself research of other experiments such as chemosynthesis of electromagnetic field environment of temperature control but also be applicable to other.
4, many sample pallets that coupling In vitro cell experiment device uses of the present invention, can regulate the height of sample table, thereby obtain suitable space, can conveniently carry out the cell sample of many group different experimental conditions tests simultaneously, can satisfy the demand of lot of experiments, and sample table adopts the irregular roundness shape design that crops the part arc length, the circuit discharging of convenient electromagnetic generating unit, also can so that thermometer can measuring vessel the range of temperature of different heights so that environment is controlled more science.
5, the structure of container of many coupling In vitro cell experiment devices of the present invention use is simple, with low cost, made by stainless steel, have the advantages that height is withstand voltage, height is airtight, easily disinfect, and adopt Modular assembled to form, make things convenient for cleaning and sterilizing and change bad, old component.
Description of drawings
Fig. 1 is the container Facad structure schematic diagram of many couplings of the present invention In vitro cell experiment device.
Fig. 2 is the container plan structure schematic diagram of many couplings of the present invention In vitro cell experiment device.
Fig. 3 is the sample pallet Facad structure schematic diagram of many couplings of the present invention In vitro cell experiment device.
Fig. 4 is sample table surface tissue schematic diagram in the sample pallet of many of the present invention coupling In vitro cell experiment device.
Fig. 5 is the electromagnetic field generator structural representation with many of the present invention coupling In vitro cell experiment matched with devices.
Wherein, 1-container, 2-blind flange, the 3-magnetic stirring apparatus, 4-sample pallet, 5-filler, the 6-dewatering outlet, 7-heating tube, 8-the first thermometer hole, the 9-ball valve, 10-air inlet port, 11-production well, 12-circuit jack, 13-weather gage hole, 14-the second thermometer hole, 15-wet bulb thermometer hole, 16-rubber cradle, 17-sample table, the 18-thread rack, 19-bracket holes, 20-boring, the 21-hex-nut, 22-Helmholtz coil, 23-parallel-plate electrode, the dull and stereotyped pallet of 24-, the 25-power supply, 26-terminal stud, 27-porous cell culture plate.
Embodiment
Embodiment 1:
As shown in Figure 1-Figure 3, many of the present embodiment coupling In vitro cell experiment devices, the blind flange 2, magnetic stirring apparatus 3 and the sample pallet 4 that comprise container 1, be complementary with container 1.
As depicted in figs. 1 and 2, described container 1 is for being equipped with the cylindrical straight tubular of insulating interlayer, it adopts stainless material to make, can stablize pressurize, the upper end of described container 1 is provided with filler 5, the lower end is provided with dewatering outlet 6, heating tube 7 and the first thermometer hole 8, inject the larger water of specific heat capacity as thermal insulation medium by filler 5,6 places, described dewatering outlet are provided with ball valve 9, the first thermometer hole 8 is placed with the first thermometer, described heating tube 7 be connected thermometer and be connected with outside temperature regulating device respectively; Described blind flange 2 is connected to the structure that but opened/closed container 1 is formed on the top of container 1, its surface is provided with air inlet port 10, production well 11, circuit jack 12, weather gage hole 13, the second thermometer hole 14 and wet bulb thermometer hole 15, described weather gage hole 13 is placed with weather gage, described the second thermometer hole 14 is placed with the second thermometer, described wet bulb thermometer hole 15 is placed with wet bulb thermometer, between blind flange 2 and container 1 top, be provided with rubber cradle 16, to improve the sealing effectiveness of container 1; Described magnetic stirring apparatus 3 is installed in the bottom of container 1, can make the water generates eddy current of container 1 interlayer, and is even to guarantee the distribution of water temperature in the container.
As shown in Figure 3 and Figure 4, described sample pallet 4 comprises four layers of sample table 17 and three thread racks 18, described every layer of sample table 17 is for cropping the irregular roundness shape of part arc length, its surface is provided with three bracket holes 19 and 16 borings 20, described bracket holes 19 is complementary with thread rack 18, described boring 20 can be placed the 15ml centrifuge tube corresponding with its diameter, and described thread rack 18 adopts stainless material to make; Thread rack 18 is passed bracket holes 19, regulate and fix the height of every layer of sample table 17 by hex-nut 21, described every layer of sample table 17 can be placed with the electromagnetic field generator of porous cell culture plate and/or single porous cell culture plate.
As shown in Figure 5, described electromagnetic field generator comprises one group of parallel Helmholtz coil 22, one group of parallel-plate electrode 23, dull and stereotyped pallet 24 and power supply 25, described parallel-plate electrode 23 is placed on the dull and stereotyped pallet 24, and described power supply 25 bottoms are provided with terminal stud 26; When electromagnetic field generator placed container 1 interior, the wiring of described terminal stud 26 was connected with the external power source control device by circuit jack 12.
Such as Fig. 1-shown in Figure 5, many coupling In vitro cell experiment processes of the present embodiment may further comprise the steps:
1) with behind container 1 sterilization and disinfection, in container 1, adds a certain amount of deionized water to keep the humidity environment of cell cultures;
The porous cell culture plate 27 that 2) will be placed with cell sample is placed on 23 of the parallel-plate electrodes of electromagnetic field generator, makes porous cell culture plate 27 can load electromagnetic field;
3) electromagnetic field generator is placed on the first layer (from top to bottom) sample table 17 of sample pallet 4, then sample pallet 4 is placed in the container 1, the wiring of terminal stud 26 is connected with the external power source control device by circuit jack 12, by blind flange 2 unopened containers 1;
4) with air inlet port 10 and the 5%CO that contains with rate of flow meter
2Medical mixed gas gas-holder link to each other, then adjust pore 11, monitor by weather gage, obtain stable air pressure;
5) closed ball valve 9 is closed dewatering outlet 6, makes container 1 be in air-tight state, injects an amount of water in filler 5, opens outside temperature regulating device, utilizes heating tube 7 heating, monitors by the first thermometer, makes water temperature constant in preset temp A;
6) after outside temperature regulating device is opened for some time, be actual temperature B by the second thermometer measuring vessel 1 interior gas temperature, by B=37 ℃ of magnetic stirring apparatus 3 control;
7) after the end, turn off first external power source control device, outside temperature regulating device and gas-holder pressurizing valve, re-adjustment production well 11 reduces container 1 internal gas pressure, opens blind flange 2 and takes out sample pallets 4.
Embodiment 2:
Many the coupling In vitro cell experiment process principal features of the present embodiment are: step 3), regulate the hex-nut 21 of sample pallet 4, obtain suitable space, electromagnetic field generator is placed on the first layer (from top to bottom) sample table 17 of sample pallet 4, simultaneously the single porous cell culture plate that is placed with cell sample is placed on the second layer sample table 17 of sample pallet 4, namely realize making the cell sample experiment of electricity consumption, magnetic, many couplings of power, also realized the cell sample experiment in the independent use field of force.All the other are with embodiment 1.
Embodiment 3:
Many the coupling In vitro cell experiment process principal features of the present embodiment are: step 3), regulate the hex-nut 21 of sample pallet 4, obtain suitable space, electromagnetic field generator is placed on the first layer (from top to bottom) sample table 17 of sample pallet 4, simultaneously the single porous cell culture plate that is placed with cell sample is placed on the second layer sample table 17 of sample pallet 4, and the 15ml centrifuge tube that will be placed with cell sample is placed in the boring 20 of the 3rd layer of sample table 17 of sample pallet 4, experiment when realizing the various kinds of cell sample.All the other are with embodiment 1.
The above; only be patent preferred embodiment of the present invention; but the protection domain of patent of the present invention is not limited to this; anyly be familiar with those skilled in the art in the disclosed scope of patent of the present invention; the design of the technical scheme of patent and patent of invention thereof is equal to replacement or is changed according to the present invention, all belongs to the protection domain of patent of the present invention.
Claims (10)
1. many coupling In vitro cell experiment devices is characterized in that: but comprise the container of constant control water temperature, be complementary with container and the surface be provided with a plurality of perforates blind flange, can realize the uniform magnetic stirring apparatus of distribution of water temperature and can place sample pallet with the electromagnetic field generator of porous cell culture plate and/or single porous cell culture plate; The top and bottom of described container are respectively arranged with filler and dewatering outlet, and place, described dewatering outlet is provided with ball valve, but described blind flange is connected to the structure that the opened/closed container is formed on the top of container, and described magnetic stirring apparatus is installed in the bottom of container; Described sample pallet places in the container when experiment.
2. many coupling In vitro cell experiment devices according to claim 1, it is characterized in that: described container lower end also is provided with heating tube and be used for places the first thermometer hole of the first thermometer, described heating tube be connected thermometer and be connected with outside temperature regulating device respectively.
3. many coupling In vitro cell experiment devices according to claim 2, it is characterized in that: the perforate that described blind flange surface arranges comprises air inlet port, production well, circuit jack, is used for placing barometric weather gage hole, is used for placing the second thermometer hole of the second thermometer and is used for placing hygroscopic wet bulb thermometer hole.
4. many coupling In vitro cell experiment devices according to claim 3, it is characterized in that: described electromagnetic field generator comprises one group of parallel Helmholtz coil, one group of parallel-plate electrode, dull and stereotyped pallet and power supply, described parallel-plate electrode is placed on the dull and stereotyped pallet, and described power supply bottom is provided with terminal stud; In the time of in electromagnetic field generator places container, the wiring of described terminal stud is connected with the external power source control device by the circuit jack.
5. many coupling In vitro cell experiment devices according to claim 1, it is characterized in that: described sample pallet comprises Multilayer Samples platform and at least three thread racks that are used for supporting sample table, described every layer of sample table is the irregular roundness shape that crops the part arc length, its surface is provided with at least three bracket holes that are complementary with thread rack, and described thread rack passes behind the bracket holes to be regulated by hex-nut and the height of fixed sample platform.
6. many coupling In vitro cell experiment devices according to claim 5, it is characterized in that: the surface of described every layer of sample table also is provided with a plurality of borings for placing centrifuge tube.
7. each described many coupling In vitro cell experiment devices according to claim 1-6, it is characterized in that: described container is the cylindrical straight tubular that insulating interlayer is housed, and it adopts stainless material to make.
8. each described many coupling In vitro cell experiment devices is characterized in that: be provided with rubber cradle between the top of described blind flange and container according to claim 1-6.
9. based on many coupling In vitro cell experiment methods of the described device of claim 4, it is characterized in that may further comprise the steps:
1) with after the container sterilization sterilization, in container, adds a certain amount of deionized water to keep the humidity environment of cell cultures;
The porous cell culture plate that 2) will be placed with cell sample is placed between the parallel-plate electrode of electromagnetic field generator;
3) electromagnetic field generator is placed on the sample pallet, then the sample pallet is placed in the container, the circuit jack is passed through in the wiring of terminal stud be connected with the external power source control device, by the blind flange unopened container;
4) with air inlet port with the 5%CO that contains of rate of flow meter
2Medical mixed gas gas-holder link to each other, then adjust pore, monitor by weather gage, obtain stable air pressure;
5) closed ball valve is closed the dewatering outlet, makes container be in air-tight state, injects an amount of water in the filler, opens outside temperature regulating device, utilizes the heating tube heating, monitors by the first thermometer, makes water temperature constant in preset temp A;
6) after outside temperature regulating device is opened for some time, be actual temperature B by gas temperature in the second thermometer measuring vessel, by B=37 ℃ of magnetic stirring apparatus control;
7) after the end, turn off first external power source control device, outside temperature regulating device and gas-holder pressurizing valve, the re-adjustment production well reduces the container internal gas pressure, opens blind flange and takes out the sample pallet.
10. many coupling In vitro cell experiment methods according to claim 9, it is characterized in that: step 3) described when being placed on electromagnetic field generator on the sample pallet, simultaneously the single porous cell culture plate that is placed with cell sample is placed on the sample pallet.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015140411A3 (en) * | 2014-03-21 | 2015-11-12 | Magnasense Technologies Oy | Coil arrangement for sample measurement with a spatially variable magnetic field |
| CN105445192A (en) * | 2015-12-14 | 2016-03-30 | 天津港东科技发展股份有限公司 | Embedded magnetic stirring constant-temperature sample rack |
| CN107102217A (en) * | 2017-04-25 | 2017-08-29 | 广东电网有限责任公司电力科学研究院 | A kind of power frequency electromagnetic field animal experiment system |
| CN109596670A (en) * | 2018-12-08 | 2019-04-09 | 北京理工大学 | A kind of electric/magnetic field environment comprehensive generating system |
| WO2022062376A1 (en) * | 2020-09-25 | 2022-03-31 | 南通纺织丝绸产业技术研究院 | Cell culture device with micro-current stimulation function |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101274101A (en) * | 2008-05-21 | 2008-10-01 | 中国农业大学 | Novel high-density carbon dioxide sterilization reactor |
| CN201271585Y (en) * | 2008-09-26 | 2009-07-15 | 杭州博日科技有限公司 | Constant temperature trough with magnetic stirring |
| CN202047072U (en) * | 2011-04-20 | 2011-11-23 | 四川大学 | Cell loading device with static magnetic field |
| CN102653719A (en) * | 2012-04-28 | 2012-09-05 | 吉林大学 | Cell culture device capable of generating multiple magnetic fields and culture method |
-
2013
- 2013-07-17 CN CN201310304190.1A patent/CN103361270B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101274101A (en) * | 2008-05-21 | 2008-10-01 | 中国农业大学 | Novel high-density carbon dioxide sterilization reactor |
| CN201271585Y (en) * | 2008-09-26 | 2009-07-15 | 杭州博日科技有限公司 | Constant temperature trough with magnetic stirring |
| CN202047072U (en) * | 2011-04-20 | 2011-11-23 | 四川大学 | Cell loading device with static magnetic field |
| CN102653719A (en) * | 2012-04-28 | 2012-09-05 | 吉林大学 | Cell culture device capable of generating multiple magnetic fields and culture method |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015140411A3 (en) * | 2014-03-21 | 2015-11-12 | Magnasense Technologies Oy | Coil arrangement for sample measurement with a spatially variable magnetic field |
| US11041828B2 (en) | 2014-03-21 | 2021-06-22 | Magnasense Technologies Oy | Coil arrangement for sample measurement with a spatially variable magnetic field |
| CN105445192A (en) * | 2015-12-14 | 2016-03-30 | 天津港东科技发展股份有限公司 | Embedded magnetic stirring constant-temperature sample rack |
| CN107102217A (en) * | 2017-04-25 | 2017-08-29 | 广东电网有限责任公司电力科学研究院 | A kind of power frequency electromagnetic field animal experiment system |
| CN109596670A (en) * | 2018-12-08 | 2019-04-09 | 北京理工大学 | A kind of electric/magnetic field environment comprehensive generating system |
| WO2022062376A1 (en) * | 2020-09-25 | 2022-03-31 | 南通纺织丝绸产业技术研究院 | Cell culture device with micro-current stimulation function |
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