CN104004656B - The cell in vitro experimental installation that Multi-angle ultrasound ripple loads and method - Google Patents
The cell in vitro experimental installation that Multi-angle ultrasound ripple loads and method Download PDFInfo
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- CN104004656B CN104004656B CN201410227850.5A CN201410227850A CN104004656B CN 104004656 B CN104004656 B CN 104004656B CN 201410227850 A CN201410227850 A CN 201410227850A CN 104004656 B CN104004656 B CN 104004656B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
Abstract
The invention discloses cell in vitro experimental installation and the method for the loading of a kind of Multi-angle ultrasound ripple, described device comprises ultrasonic generator and at least one the ultrasonic pressing equipment be connected with ultrasonic generator; Described every platform ultrasonic pressing equipment comprises housing and many spill rotary screws, described every bar spill rotary screw comprises rotating head, main stock, first connecting rod and second connecting rod, described main stock is provided with multiple ultrasonic emitting probe, and the multiple ultrasonic emitting on every bar spill rotary screw are popped one's head in the ultrasonic emitting probe of the arrangement of forming array together; When cell cultures porous plate is fixed on housing upper, described each ultrasonic emitting probe can at the center, each hole of any theta alignment cell cultures porous plate.Apparatus of the present invention and method can the impacts of testing cell single culture or biomaterial and cell co-culture of the ul-trasonic irradiation of the Study of the Realization different incidence angles, carry out, improve the efficiency of experimentation while can meeting many group experiments.
Description
Technical field
The present invention relates to a kind of experimental installation of cytobiology, the cell in vitro experimental installation that especially a kind of Multi-angle ultrasound ripple loads and method, belong to medical instruments field.
Background technology
Ultrasonicly to be widely used at medical field.Ultrasonicly can be used for the operation of warding off stone, removal lesion tissue's (as cataract), in joint replacement overhaul technology, remove bone cement; When clinical treatment is applied, heat energy can being produced in the tissue, for easing the pain, relieve muscle spasms, improving joint and muscle activity; Low intensity ultrasound device, especially for imaging of medical, carries out imaging to vitals, fetus, eyeball and osseous tissue etc. in body; Also there are some researches show that low intensity ultrasound can be used for promoting union of fracture etc.
Ultrasonic wave is a kind of acoustic energy exceeding people's audibility range, and it propagates the change that can cause in-vivo tissue, cell or biomolecules function and metabolism in vivo.It is generally acknowledged that ultrasound treatment is coefficient by mechanical effect, thermogenetic effect and physical and chemical effect.On the one hand, the scholars such as Carter think that low intensity ultrasound is as a kind of acoustic energy, will be converted into weak mechanical energy and produce mechanical stimulation to fracture tissue and cell, thus promote union of fracture when acting on fracture site; On the other hand, the scholars such as Rawool think that ultrasonic thermogenetic effect adds the blood confession of damage location, thus add somatomedin and the cytokine of damage location tissue, are beneficial to and organize self-regeneration; In addition, there is scholar to find, ultrasonicly can change membrane passage, the ion metabolism inside and outside the change cytolemma of permeability of cell membrane.At research low intensity ultrasound in the research of union of fracture, Ryaby etc. report low intensity ultrasound can promote calcium ion and the cartilage broken up, osteocyte combines, add the activity of second messenger, improve adenylate cyclase activity and scleroblast to the synthesis of TGF-β, the synthesis of accelerated bone.In a word, hyperacoustic loading is as a kind of ectogenic physical stimulation, on the cell in biological tissue, enzyme, biomolecules metabolism has affects closely, but, utilize ultrasonic wave extensively to study the cell in vitro experiment of various biomaterial still not, the inherent mechanism of ultrasonication is still unclear.
At present, for study ultrasonic pressing on the experimental installation of the impact of cell proliferation in cell cultivation process, adhesion, differentiation mainly single in a culture dish or airtight chamber by liquid conductive ultrasonic wave, but, to there is operation in this device more loaded down with trivial details in experimentation, be unfavorable for that batch repeats experiment, experimental situation is difficult to reach the defects such as cell cultures requirement.
Summary of the invention
The object of the invention is the defect in order to solve above-mentioned prior art, provide the cell in vitro experimental installation that a kind of Multi-angle ultrasound ripple loads, this apparatus structure is simple, with low cost, easy to operate, diverse in function, can the impact that cell single culture or biomaterial and cell co-culture are tested of the ul-trasonic irradiation of the Study of the Realization different incidence angles, carry out while can meeting many group experiments, improve the efficiency of experimentation.
The cell in vitro experimental installation that another object of the present invention is to provide a kind of above-mentioned Multi-angle ultrasound ripple to load carries out the method for cell cultures.
Object of the present invention can reach by taking following technical scheme:
The cell in vitro experimental installation that Multi-angle ultrasound ripple loads, comprises ultrasonic generator and at least one the ultrasonic pressing equipment be connected with ultrasonic generator; Described every platform ultrasonic pressing equipment comprises housing and many spill rotary screws, described every bar spill rotary screw comprises rotating head, main stock, first connecting rod and second connecting rod, the outside that one end of described first connecting rod extends to housing front portion in housing is connected with rotating head, one end of described second connecting rod extends to the outside at housing rear portion in housing, and described first connecting rod is connected with main stock respectively with the other end of second connecting rod; Described main stock is provided with multiple ultrasonic emitting probe, multiple ultrasonic emitting on every bar spill rotary screw are popped one's head in the ultrasonic emitting probe of the arrangement of forming array together, described housing upper is provided with multiple through holes of array arrangement, each hole one_to_one corresponding of described each through hole and cell cultures porous plate; When cell cultures porous plate is fixed on housing upper, described each ultrasonic emitting probe can at the arbitrarily angled center, each hole by each through-hole alignment cell cultures porous plate.
As a kind of preferred version, described every platform ultrasonic pressing equipment also comprises multiple angle dial plate, described each angle dial plate and every bar spill rotary screw one_to_one corresponding, and be arranged on the outer side of housing front portion, the outer side of described housing front portion is also provided with and multiple angle dial plate multiple opening one to one, the main stock of described every bar spill rotary screw extends to opening part in housing, reads the angle of rotation of spill rotary screw with this.
As a kind of preferred version, described ultrasonic generator inside is provided with ultrasonic wave generation control single chip computer group, anterior outer side is provided with ultrasonic power control knob, ultrasonic frequency control knob, ultrasonic wave wavelength control knob, ul-trasonic irradiation time controling knob and with each control knob four digital display windows one to one, described ultrasonic wave generation control single chip computer group by circuit respectively with ultrasonic power control knob, ultrasonic frequency control knob, ultrasonic wave wavelength control knob, ul-trasonic irradiation time controling knob is connected with four digital display windows.
As a kind of preferred version, described every platform ultrasonic pressing equipment also comprises circuit card, and described circuit card is arranged on the lower position in housing, and described multiple ultrasonic emitting probe is connected with ultrasonic generator by circuit card.
As a kind of preferred version, described device also comprises two elastic brace webs, the two ends of described every bar elastic brace web are furnished with shackle, and described two elastic brace webs snap over the two ends of housing front portion and the two ends at housing rear portion respectively by shackle, and cell cultures porous plate is fixed on housing.
As a kind of preferred version, described device also comprises cell culture incubator, and the multilayer being provided with genesis analysis in described cell culture incubator cultivates platform, cultivates platform for placing a ultrasonic pressing equipment for described every layer.
As a kind of preferred version, the rotation angle range of described every bar spill rotary screw is 0 ~ 180 °.
As a kind of preferred version, when cell cultures porous plate is fixed on housing upper, the rotation centerline of described many spill rotary screws is positioned in cell cultures porous plate hole inner bottom part plane.
As a kind of preferred version, outside the revolution space of the inherent spill rotary screw of described housing, be partially filled sound-absorbing material.
Another object of the present invention can reach by taking following technical scheme:
The cell in vitro experimental installation that Multi-angle ultrasound ripple loads carries out the method for cell cultures, comprises the following steps:
1) one or more ultrasonic loading equipemtn is put into cell culture incubator after ethylene oxide sterilizing, more ultrasonic loading equipemtn is connected with the ultrasonic generator outside cell culture incubator;
2) cell cultures porous plate is fixed on ultrasonic loading equipemtn, by rotating spill rotary screw, regulates the hyperacoustic incident angle in experimentation, then closing cell culture incubator;
3) open the power supply of ultrasonic generator, by power, frequency, wavelength and action time four control knobs experiment desired parameters is set, start experiment;
4) after experiment terminates, close the power supply of ultrasonic generator, take out cell cultures porous plate and carry out cytologic experiment detection, and remove the connection of ultrasonic generator and ultrasonic pressing equipment, take out ultrasonic pressing equipment, close cell culture incubator, complete experiment.
The present invention has following beneficial effect relative to prior art:
1, the cell in vitro experimental installation that Multi-angle ultrasound ripple of the present invention loads uses independently ultrasonic generator, in experimentation, can be placed on outside cell culture incubator, solve device sterilizing difficulty, space is comparatively large, not easily moves, is unsuitable for the difficult problems such as high humidity environment.
2, in the cell in vitro experimental installation that Multi-angle ultrasound ripple of the present invention loads, ultrasonic pressing equipment is separated with ultrasonic generator, independently uses, in experimentation, ultrasonic pressing equipment is put into cell culture incubator and is tested together with cell cultures porous plate.
3, in the cell in vitro experimental installation that Multi-angle ultrasound ripple of the present invention loads, ultrasonic pressing equipment is provided with the ultrasonic probe of array arrangement, each ultrasonic probe is perpendicular to the main stock of rotary screw, its generating plane is in rotary screw plane, each probe is corresponding with the respective aperture of cell cultures porous plate in any angle of rotation, carries out while can realizing the experiment of many group different parameters.
4, in the cell in vitro experimental installation that Multi-angle ultrasound ripple of the present invention loads, the part sound-absorbing material of ultrasonic pressing equipment in housing outside the revolution space of spill rotary screw is filled, and prevents the interference of ultrasonic wave to experimental result of reflection.
5, in the cell in vitro experimental installation that Multi-angle ultrasound ripple of the present invention loads, when cell cultures porous plate is fixed on the housing upper of ultrasonic pressing equipment, the rotation centerline of described many spill rotary screws is positioned in cell cultures porous plate hole inner bottom part plane, makes ul-trasonic irradiation region at the center, each hole of cell cultures porous plate.
6, the cell in vitro experimental installation applied range of Multi-angle ultrasound ripple loading of the present invention, both can the impact that cell single culture is tested of the ul-trasonic irradiation of the Study of the Realization 0 ~ 90 ° of input angle, also can the impact that biomaterial and cell co-culture are tested of the ul-trasonic irradiation of the Study of the Realization 0 ~ 90 ° of input angle.
7, the cell in vitro experimental installation of Multi-angle ultrasound ripple loading of the present invention, structure is simple, with low cost, easy to operate, diverse in function, carries out, improve the efficiency of experimentation while can meeting many group experiments.
Accompanying drawing explanation
The relation schematic diagram of cell culture incubator, ultrasonic generator and ultrasonic pressing equipment when Fig. 1 is the cell in vitro experimental installation experiment of Multi-angle ultrasound ripple of the present invention loading.
Fig. 2 is the Facad structure figure of the ultrasonic generator of the cell in vitro experimental installation that Multi-angle ultrasound ripple of the present invention loads.
Fig. 3 is the left part skeleton view of the ultrasonic generator of the cell in vitro experimental installation that Multi-angle ultrasound ripple of the present invention loads.
Fig. 4 is the Facad structure figure that the ultrasonic pressing equipment of cell in vitro experimental installation that Multi-angle ultrasound ripple of the present invention loads directly installs cell cultures porous plate.
Fig. 5 is the left part perspective structure figure that the ultrasonic pressing equipment of cell in vitro experimental installation that Multi-angle ultrasound ripple of the present invention loads directly installs cell cultures porous plate.
Fig. 6 is the ultrasonic pressing equipment sheet of biomaterial of cell in vitro experimental installation and the Facad structure figure of cell co-culture of Multi-angle ultrasound ripple of the present invention loading.
Fig. 7 is the ultrasonic pressing equipment sheet of biomaterial of cell in vitro experimental installation and the left part perspective structure figure of cell co-culture of Multi-angle ultrasound ripple of the present invention loading.
Wherein, 1-cell culture incubator, 2-ultrasonic generator, 3-ultrasonic pressing equipment, 4-elastic brace web, 5-cultivates platform, 6-ultrasonic wave generation control single chip computer group, 7-ultrasonic power control knob, 8-ultrasonic frequency control knob, 9-ultrasonic wave wavelength control knob, 10-ul-trasonic irradiation time controling knob, 11-numerical monitor window, 12-cell cultures porous plate, 13-housing, 14-circuit card, 15-angle dial plate, 16-spill rotary screw, 17-opening, 18-rotating head, the main stock of 19-, 20-first connecting rod, 21-second connecting rod, 22-ultrasonic emitting is popped one's head in, 23-through hole, 24-sound-absorbing material, 25-sheet of biomaterial, 26-secure silicon film.
Embodiment
Embodiment 1:
As shown in Fig. 1 ~ Fig. 7, the cell in vitro experimental installation of the Multi-angle ultrasound ripple loading of the present embodiment, comprises cell culture incubator 1, ultrasonic generator 2, three ultrasonic pressing equipment 3 and two elastic brace webs 4; Cultivate platform 5 for three layers that are provided with genesis analysis in described cell culture incubator 1, described every layer of cultivation platform 5 is all connected with ultrasonic generator 2 for placing a ultrasonic pressing equipment 3, three ultrasonic pressing equipment 3.
Described ultrasonic generator 2 inside is provided with ultrasonic wave generation control single chip computer group 6, anterior outer side is provided with ultrasonic power control knob 7, ultrasonic frequency control knob 8, ultrasonic wave wavelength control knob 9, ul-trasonic irradiation time controling knob 10 and with four control knobs, four digital display windows 11 one to one, described ultrasonic wave generation control single chip computer group 6 by circuit respectively with ultrasonic power control knob 7, ultrasonic frequency control knob 8, ultrasonic wave wavelength control knob 9, ul-trasonic irradiation time controling knob 10 is connected with four digital display windows 11, described ultrasonic wave generation control single chip computer group 6 can produce the low-intensity ultrasonic for ultrasound in medicine and biology imaging, intensity is 1 ~ 50mW/cm
2, also can produce the ultrasonic wave of clinical treatment, intensity is 1 ~ 3W/cm
2.
The cell cultures porous plate 12 that the present embodiment adopts has 12 hole (not shown)s of array arrangement, each shows three holes, when carrying out independent cell cultures, the biomaterial of powder, film, support and so on and the laboratory of co-culture of cells directly can use the cell cultures porous plate that market is bought, and when studying sheet compact material as tinsel, ceramic plate, preferably use through hole cell cultures porous plate.
Described every platform ultrasonic pressing equipment 3 comprises housing 13, circuit card 14, four angle dial plates 15 and four spill rotary screws 16, described circuit card 14 is arranged on the lower position in housing 13, described each angle dial plate 15 and every bar spill rotary screw 16 one_to_one corresponding, and be arranged on the outer side of housing 13 front portion, the outer side of described housing 13 front portion is also provided with and four angle dial plates 15, four openings 17 one to one; Described every bar spill rotary screw 16 comprises rotating head 18, main stock 19, first connecting rod 20 and second connecting rod 21, described main stock 19 extends to opening 17 in housing 13, the outside that one end of described first connecting rod 20 extends to housing 13 front portion in housing 13 is connected with rotating head 18, one end of described second connecting rod 21 extends to the outside at housing 13 rear portion in housing 13, and described first connecting rod 20 is connected with main stock 19 respectively with the other end of second connecting rod 21; Under the drive of rotating head 18, main stock 19, first connecting rod 20 and second connecting rod 21 can be made to rotate simultaneously.
Described main stock 19 is provided with three ultrasonic emitting probe 22, and three ultrasonic emitting on every bar spill rotary screw 16 are popped one's head in the ultrasonic emitting probe 22 of 22 forming arrays arrangements, namely have 12 ultrasonic emitting probes 22; Described housing 13 top is provided with 12 through holes 23 of array arrangement, each hole one_to_one corresponding of described each through hole and cell cultures porous plate 12, described each ultrasonic emitting probe 22 aims at the center, each hole of cell cultures porous plate 12 by each through hole 23, and is connected with ultrasonic generator 2 by circuit card 14; The rotation angle range of described every bar spill rotary screw 16 is 0 ~ 180 °, and ultrasonic emitting probe 22 is rotated around the rotation centerline (i.e. the horizontal center line of rotating head 18) of spill rotary screw 16 with 0 ~ 180 ° (semicircle angle); The rotation centerline of described spill rotary screw 16 is positioned in cell cultures porous plate endoporus base plane, and ultrasonic wave is just in time acted on cell.Be partially filled sound-absorbing material 24 outside the revolution space of the inherent spill rotary screw 16 of described housing 13, prevent the interference of ultrasonic wave to experimental result of reflection.
Described every bar elastic brace web 4 is high temperature resistant (can bear sterilizing high temperature) elastic reducing-fixing band, its two ends are furnished with shackle, described two elastic brace webs 4 snap over the two ends of housing 13 front portion and the two ends at housing 13 rear portion respectively by shackle, and cell cultures porous plate 12 is fixed on housing 13.
As shown in Fig. 1 ~ Fig. 5, the experimentation of the present embodiment, comprises the following steps:
1) ultrasonic loading equipemtn 3 is put into cell culture incubator 1 after ethylene oxide sterilizing, more ultrasonic loading equipemtn is connected with the ultrasonic generator 2 outside cell culture incubator 1;
2) if carry out cell single culture, cell cultures porous plate 12 is directly fixed on housing 13 top, as shown in Figure 6; If carry out the co-cultivation of sheet of biomaterial 25 and cell, secure silicon film 26 pairs of sheet of biomaterial 25 are utilized to seal, secure silicon film 26 and sheet of biomaterial 25 entirety are placed on each through hole 23 on housing 13 top, again cell cultures porous plate 12 is fixed, as shown in Figure 7; Then by rotating spill rotary screw 16, regulating the hyperacoustic incident angle (0 ~ 90 °) in experimentation, then closing cell culture incubator 1;
3) open the power supply of ultrasonic generator 2, by power, frequency, wavelength and action time four control knobs experiment desired parameters is set, start experiment;
4) after experiment terminates, close the power supply of ultrasonic generator 2, take out cell cultures porous plate 12 and carry out cytologic experiment detection, and remove the connection of ultrasonic generator 2 and ultrasonic pressing equipment 3, take out ultrasonic pressing equipment 3, close cell culture incubator 1, complete experiment.
In sum, the cell in vitro experimental installation that Multi-angle ultrasound ripple of the present invention loads can the impact of testing cell single culture or biomaterial and cell co-culture of the ul-trasonic irradiation of the Study of the Realization different incidence angles, carry out while can meeting many group experiments, improve the efficiency of experimentation.
The above; be only patent preferred embodiment of the present invention; but the protection domain of patent of the present invention is not limited thereto; such as the quantity of angle dial plate, spill rotary screw, ultrasonic emitting probe can adjust according to actual needs; the angle of rotation of spill rotary screw also can be in the scope of 15 ~ 165 ° etc.; anyly be familiar with those skilled in the art in the scope disclosed in patent of the present invention; be equal to according to the technical scheme of patent of the present invention and patent of invention design thereof and replaced or change, all belonged to the protection domain of patent of the present invention.
Claims (10)
1. the cell in vitro experimental installation of Multi-angle ultrasound ripple loading, is characterized in that: comprise ultrasonic generator and at least one the ultrasonic pressing equipment be connected with ultrasonic generator; Described every platform ultrasonic pressing equipment comprises housing and many spill rotary screws, described every bar spill rotary screw comprises rotating head, main stock, first connecting rod and second connecting rod, the outside that one end of described first connecting rod extends to housing front portion in housing is connected with rotating head, one end of described second connecting rod extends to the outside at housing rear portion in housing, and described first connecting rod is connected with main stock respectively with the other end of second connecting rod; Described main stock is provided with multiple ultrasonic emitting probe, multiple ultrasonic emitting on every bar spill rotary screw are popped one's head in the ultrasonic emitting probe of the arrangement of forming array together, described housing upper is provided with multiple through holes of array arrangement, each hole one_to_one corresponding of described each through hole and cell cultures porous plate; When cell cultures porous plate is fixed on housing upper, described each ultrasonic emitting probe can at the arbitrarily angled center, each hole by each through-hole alignment cell cultures porous plate.
2. the cell in vitro experimental installation of Multi-angle ultrasound ripple loading according to claim 1, it is characterized in that: described every platform ultrasonic pressing equipment also comprises multiple angle dial plate, described each angle dial plate and every bar spill rotary screw one_to_one corresponding, and be arranged on the outer side of housing front portion, the outer side of described housing front portion is also provided with and multiple angle dial plate multiple opening one to one, the main stock of described every bar spill rotary screw extends to opening part in housing.
3. the cell in vitro experimental installation of Multi-angle ultrasound ripple loading according to claim 1 and 2, it is characterized in that: described ultrasonic generator inside is provided with ultrasonic wave generation control single chip computer group, anterior outer side is provided with ultrasonic power control knob, ultrasonic frequency control knob, ultrasonic wave wavelength control knob, ul-trasonic irradiation time controling knob and with each control knob four digital display windows one to one, described ultrasonic wave generation control single chip computer group by circuit respectively with ultrasonic power control knob, ultrasonic frequency control knob, ultrasonic wave wavelength control knob, ul-trasonic irradiation time controling knob is connected with four digital display windows.
4. the cell in vitro experimental installation of Multi-angle ultrasound ripple loading according to claim 1 and 2, it is characterized in that: described every platform ultrasonic pressing equipment also comprises circuit card, described circuit card is arranged on the lower position in housing, and described multiple ultrasonic emitting probe is connected with ultrasonic generator by circuit card.
5. the cell in vitro experimental installation of Multi-angle ultrasound ripple loading according to claim 1 and 2, it is characterized in that: described device also comprises two elastic brace webs, the two ends of described every bar elastic brace web are furnished with shackle, described two elastic brace webs snap over the two ends of housing front portion and the two ends at housing rear portion respectively by shackle, and cell cultures porous plate is fixed on housing.
6. the cell in vitro experimental installation of Multi-angle ultrasound ripple loading according to claim 1 and 2, it is characterized in that: described device also comprises cell culture incubator, the multilayer being provided with genesis analysis in described cell culture incubator cultivates platform, cultivates platform for placing a ultrasonic pressing equipment for described every layer.
7. the cell in vitro experimental installation of Multi-angle ultrasound ripple loading according to claim 1 and 2, is characterized in that: the rotation angle range of described every bar spill rotary screw is 0 ~ 180 °.
8. the cell in vitro experimental installation of Multi-angle ultrasound ripple loading according to claim 1 and 2, it is characterized in that: when cell cultures porous plate is fixed on housing upper, the rotation centerline of described many spill rotary screws is positioned in cell cultures porous plate hole inner bottom part plane.
9. the cell in vitro experimental installation that loads of Multi-angle ultrasound ripple according to claim 1 and 2, is characterized in that: be partially filled sound-absorbing material outside the revolution space of described housing inherence spill rotary screw.
10. the cell in vitro experimental installation loaded based on Multi-angle ultrasound ripple described in claim 3 carries out the method for cell cultures, it is characterized in that comprising the following steps:
1) one or more ultrasonic loading equipemtn is put into cell culture incubator after ethylene oxide sterilizing, more ultrasonic loading equipemtn is connected with the ultrasonic generator outside cell culture incubator;
2) cell cultures porous plate is fixed on ultrasonic loading equipemtn, by rotating spill rotary screw, regulates the hyperacoustic incident angle in experimentation, then closing cell culture incubator;
3) open the power supply of ultrasonic generator, by power, frequency, wavelength and action time four control knobs experiment desired parameters is set, start experiment;
4) after experiment terminates, close the power supply of ultrasonic generator, take out cell cultures porous plate and carry out cytologic experiment detection, and remove the connection of ultrasonic generator and ultrasonic pressing equipment, take out ultrasonic pressing equipment, close cell culture incubator, complete experiment.
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| CN110760441B (en) * | 2019-09-25 | 2021-08-03 | 浙江大学 | Method and device for generating ultrasonic radiation force field for in-vitro culture of engineering cartilage |
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| US6699711B1 (en) * | 1998-05-07 | 2004-03-02 | Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Device and method for selective exposure of a biological sample to sound waves |
| JP2005160340A (en) * | 2003-12-01 | 2005-06-23 | Teijin Pharma Ltd | Cell culture apparatus and cell culture method |
| CN202610237U (en) * | 2012-06-18 | 2012-12-19 | 陈智毅 | Operating platform beneficial for ultrasound mediating gene transfection |
| CN203866327U (en) * | 2014-05-27 | 2014-10-08 | 华南理工大学 | Multi-angle ultrasonic-pressing in-vitro cytology experimental device |
-
2014
- 2014-05-27 CN CN201410227850.5A patent/CN104004656B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6699711B1 (en) * | 1998-05-07 | 2004-03-02 | Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. | Device and method for selective exposure of a biological sample to sound waves |
| JP2005160340A (en) * | 2003-12-01 | 2005-06-23 | Teijin Pharma Ltd | Cell culture apparatus and cell culture method |
| CN202610237U (en) * | 2012-06-18 | 2012-12-19 | 陈智毅 | Operating platform beneficial for ultrasound mediating gene transfection |
| CN203866327U (en) * | 2014-05-27 | 2014-10-08 | 华南理工大学 | Multi-angle ultrasonic-pressing in-vitro cytology experimental device |
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