CN103356741B - Natural medicine composition - Google Patents
Natural medicine composition Download PDFInfo
- Publication number
- CN103356741B CN103356741B CN201310249494.2A CN201310249494A CN103356741B CN 103356741 B CN103356741 B CN 103356741B CN 201310249494 A CN201310249494 A CN 201310249494A CN 103356741 B CN103356741 B CN 103356741B
- Authority
- CN
- China
- Prior art keywords
- radix scutellariae
- preparation
- ethanol
- drug composition
- natural drug
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a natural medicine composition. The natural medicine composition comprises a herba andrographitis and radix scutellariae decoction and concentration extractive, herba andrographitis lactone, radix scutellariae total flavone and baicalin in proportion of (2-5): (3-6): (1-6): (5-8). The natural medicine composition has a very good curative effect on cancer chemotherapy related diarrhea and meanwhile has a synergistic effect on cancer chemotherapy.
Description
Technical field
The present invention relates to a kind of natural drug composition, relate more specifically to a kind of natural drug composition be made up of Herba Andrographis Radix Scutellariae water boiling concentration extract, andrographolide, Radix Scutellariae total flavones and baicalin, the preparation method of this natural drug composition and this natural drug composition are for the preparation of minimizing cancer chemotherapy induced diarrhea and the purposes risen treatment of cancer in the medicine of potentiation.
Background technology
Malignant tumor is the existence of current harm humans, and one of healthy principal disease, its treatment means comprises operation, chemotherapy, radiotherapy, Chinese medicine, and the effect of chemotherapeutic treatment tumor is particularly outstanding.No matter alone chemotherapeutic is or combine with the toxicity such as all can cause that patient suffers from diarrhoea, and suffers from diarrhoea and be Chemotherapy-induced Diarrhea after chemotherapy of tumors.In recent years along with chemotherapeutics emerging and applying as 5-fluorouracil, taxanes, hydroxy camptothecin, irinotecan, xeloda etc., the incidence rate of Chemotherapy-induced Diarrhea improves year by year, if do not carry out active treatment, easily cause that patient dewaters, the disorder of malnutrition, serum electrolyte, acid base imbalance etc., it is unfavorable greatly not only to bring the rehabilitation of body, also bring certain difficulty to CC, therefore effective treatment of Chemotherapy-induced Diarrhea has been become to the important component part of tumor patient wholistic therapy.
Chemotherapy-induced Diarrhea is with regard to its clinical manifestation, and surely belong to Chinese medicine and " to have loose bowels " category, Chinese medicine, for the research of having loose bowels, attracts people's attention, but there is no the Chinese patent medicine that antagonism cancer chemotherapy has alleviating diarrhoea and potentiation at present.Therefore, research and develop a kind of high-efficiency low-toxicity, cheap, be easy to promote can be used for alleviating cancer chemotherapy induced diarrhea and the natural Chinese medicine playing chemotherapeutic sensitivity effect becomes clinical problem demanding prompt solution.
Herba Andrographis is herb or the leaf of acanthaceous plant Herba Andrographis, and nature and flavor bitter cold, cures mainly damp-heat dysentery.Radix Scutellariae is the root of labiate Radix Scutellariae, cure mainly dysentery, in recent years, Herba Andrographis and Radix Scutellariae antitumaous effect are confirmed, the present inventor is through the research of a large amount of each formula proportion and experiment, finally obtain optimal drug comparative compositions of the present invention, and demonstrate it and have fabulous curative effect simultaneously to chemotherapeutic sensitivity, the present invention can be this completes to cancer chemotherapy induced diarrhea.
Summary of the invention
A kind of natural drug composition provided by the invention, is made up of in the ratio of 2-5:3-6:1-6:5-8 by weight Herba Andrographis Radix Scutellariae water boiling concentration extract, andrographolide, Radix Scutellariae total flavones and baicalin.
Above-mentioned natural drug composition, wherein the usage ratio of Herba Andrographis Radix Scutellariae water boiling concentration extract, andrographolide, Radix Scutellariae total flavones and baicalin is 5:6:6:8.
Wherein, Herba Andrographis Radix Scutellariae water boiling concentration extract, andrographolide, Radix Scutellariae total flavones are by well known to a person skilled in the art that conventional method is carried out purification and obtained, and the natural drug of the present invention that these compositions adopting conventional method to obtain or the one-tenth adopting business to buy are grouped into all can obtain identical curative effect.
Preferably, in above-mentioned natural drug composition, the preparation method of Herba Andrographis Radix Scutellariae water boiling concentration extract is:
Be 1-4: 1 get Herba Andrographis, Radix Scutellariae, distilled water immersion 1h according to proportion compatibility, heating decocts 1h, and silk filters, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, by twice filtrate mixing, heating is concentrated, ultrafiltration membrance filter, 70 DEG C of drying under reduced pressure and get final product.
In above-mentioned natural drug composition, the preparation method of andrographolide is:
Take Common Andrographis Herd appropriate, enzymolysis pre-treatment, ethanol hot dipping, filter, recovery ethanol, obtain extractum, wash with petroleum ether, removing chlorophyll, water layer chloroform extraction is spent the night, is divided into water layer, chloroform layer and intermediate layer, by intermediate layer precipitate ethyl alcohol recrystallization, to obtain final product.
In above-mentioned natural drug composition, Radix Scutellariae total flavones preparation method is:
Take Radix Scutellariae coarse powder, 55% ethanol reflux, extract, at 85 DEG C repeatedly, is filtered, merging filtrate, reclaims ethanol, concentrated, dry, to obtain final product.
In above-mentioned natural drug composition, baicalin preparation method is:
Radix Scutellariae is dried, and pulverizes and sieves, and takes appropriate, with 80% ethanol with 10 times, 8 times amount extract at twice, each 1h, filtration.Merging filtrate, reclaims ethanol, adds appropriate distilled water, adds concentrated hydrochloric acid and adjusts pH3-4, elimination impurity after standing 1h, and filtrate adjusts pH1.5-2 in 40 DEG C, and insulation 30min, leaves standstill, sucking filtration, and precipitation is washed to pH6.5-7.0, is drying to obtain baicalin.
Present invention also offers the preferred preparation method of this natural drug composition, comprising:
(1) preparation of Herba Andrographis Radix Scutellariae water boiling concentration extract
Be 1-4: 1 get Herba Andrographis, Radix Scutellariae, distilled water immersion 1h according to proportion compatibility, heating decocts 1h, and silk filters, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, by twice filtrate mixing, heating is concentrated, ultrafiltration membrance filter, 70 DEG C of drying under reduced pressure and get final product.
(2) preparation of andrographolide
Take Common Andrographis Herd appropriate, enzymolysis pre-treatment, ethanol hot dipping, filter, recovery ethanol, obtain extractum, wash with petroleum ether, removing chlorophyll, water layer chloroform extraction is spent the night, is divided into water layer, chloroform layer and intermediate layer, by intermediate layer precipitate ethyl alcohol recrystallization, to obtain final product.
(3) Radix Scutellariae total flavones preparation
Take Radix Scutellariae coarse powder, 55% ethanol reflux, extract, at 85 DEG C repeatedly, is filtered, merging filtrate, reclaims ethanol, concentrated, dry, to obtain final product.
(4) baicalin preparation
Radix Scutellariae is dried, and pulverizes and sieves, and takes appropriate, with 80% ethanol with 10 times, 8 times amount extract at twice, each 1h, filtration.Merging filtrate, reclaims ethanol, adds appropriate distilled water, adds concentrated hydrochloric acid and adjusts pH3-4, elimination impurity after standing 1h, and filtrate adjusts pH1.5-2 in 40 DEG C, and insulation 30min, leaves standstill, sucking filtration, and precipitation is washed to pH6.5-7.0, is drying to obtain baicalin.
(5) choose Herba Andrographis Radix Scutellariae water boiling concentration extract, andrographolide, Radix Scutellariae total flavones and baicalin in proportion, Homogeneous phase mixing, to obtain final product.
The preparation method of above-mentioned natural drug composition, also comprises: product obtained for step (5) is made capsule containing natural drug composition 100mg or 200mg specification or tablet further by the pharmacy means of routine.
The beneficial effect of natural drug composition of the present invention in anti-Chemotherapy-induced Diarrhea and Tumor suppression is as follows:
1, natural drug composition of the present invention, causes the research of diarrhea of mouse model to show at irinotecan: the diarrhoea reaction for irinotecan induced mice has obvious antagonism.
2, demonstrate this natural drug composition and can strengthen the inhibitory action of chemotherapeutics to tumor, have employed mouse tumor model, observe this natural drug composition and cyclophosphamide combined to the inhibitory action of mouse interior tumor.Research shows: this natural drug composition obviously can increase the inhibitory action of cyclophosphamide for tumor.
3, Herba Andrographis Radix Scutellariae water boiling concentration extract, andrographolide, Radix Scutellariae total flavones and baicalin combine by the present inventor originally, and the consumption proportion of these components is determined by great many of experiments, said composition is played good synergism, obtains unexpected effect.Pass through our experimentation repeatedly, ratio between each component of natural drug composition of the present invention creates unexpected synergism when 2-5:3-6:1-6:5-8, potentiation is obvious, extraordinary effect is played to side effect, especially especially obvious when the usage ratio of each component is 5:6:6:8, this is that the present inventor pays a large amount of creative work and obtains, and those of ordinary skill in the art cannot obtain this proportioning and optimum amount ratio only by disclosed content.
4, this natural drug composition Herba Andrographis Radix Scutellariae water boiling concentration extract, andrographolide, Radix Scutellariae total flavones and each component of baicalin all obtain by conventional means, andrographolide is also buied by business, and such compositions itself has acquisition convenience, lower-cost effect.This is also the product having splendid curative effect and higher industrial practicality concurrently that inventor is obtained by a large amount of creative work side.
Detailed description of the invention
The preparation of natural drug composition of the present invention is set forth further below by embodiment and test example, and its beneficial effect applied in diarrhea and antitumor.
Embodiment
The first step: the preparation of various extract
(1) preparation of Herba Andrographis Radix Scutellariae water boiling concentration extract
Be 1-4: 1 get Herba Andrographis, Radix Scutellariae, distilled water immersion 1h according to proportion compatibility, heating decocts 1h, and silk filters, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, by twice filtrate mixing, heating is concentrated, ultrafiltration membrance filter, 70 DEG C of drying under reduced pressure and get final product.
(2) preparation of andrographolide
Take Common Andrographis Herd appropriate, enzymolysis pre-treatment, ethanol hot dipping, filter, recovery ethanol, obtain extractum, wash with petroleum ether, removing chlorophyll, water layer chloroform extraction is spent the night, is divided into water layer, chloroform layer and intermediate layer, by intermediate layer precipitate ethyl alcohol recrystallization, to obtain final product.
(3) Radix Scutellariae total flavones preparation
Take Radix Scutellariae coarse powder, 55% ethanol reflux, extract, at 85 DEG C repeatedly, is filtered, merging filtrate, reclaims ethanol, concentrated, dry, to obtain final product.
(4) baicalin preparation
Radix Scutellariae is dried, and pulverizes and sieves, and takes appropriate, with 80% ethanol with 10 times, 8 times amount extract at twice, each 1h, filtration.Merging filtrate, reclaims ethanol, adds appropriate distilled water, adds concentrated hydrochloric acid and adjusts pH3-4, elimination impurity after standing 1h, and filtrate adjusts pH1.5-2 in 40 DEG C, and insulation 30min, leaves standstill, sucking filtration, and precipitation is washed to pH6.5-7.0, is drying to obtain.
Second step: the preparation of preparation
(1) preparation of natural drug composition capsule preparations of the present invention
Take Herba Andrographis Radix Scutellariae water boiling concentration extract 20mg prepared by above-mentioned steps, andrographolide 24mg, Radix Scutellariae total flavones 24mg and baicalin 32mg respectively, take starch 395mg, magnesium stearate 5mg, Homogeneous phase mixing simultaneously, be filled to capsule.
(2) preparation of natural drug composition tablet of the present invention
Take Herba Andrographis Radix Scutellariae water boiling concentration extract 20mg prepared by above-mentioned steps, andrographolide 24mg, Radix Scutellariae total flavones 24mg and baicalin 32mg respectively, take starch 295mg, lactose 100mg simultaneously, Homogeneous phase mixing, with wet granulation, drying, mix with 5mg magnesium stearate after granulate, be pressed into tablet.
Drug effect and pharmacological data:
In following test example, compositions is: the usage ratio of Herba Andrographis Radix Scutellariae water boiling concentration extract, andrographolide, Radix Scutellariae total flavones and baicalin is 5:6:6:8.Inventor to above-mentioned each Ingredient Amount ratio between 2-5:3-6:1-6:5-8 time the compositions that obtains of multiple proportioning combination carried out with on identical experiment, all find that the natural drug composition in this amount ranges can obtain good synergism, good effect can be played to cancer chemotherapy induced diarrhea and have potentiation to the treatment of cancer.Below the pharmacodynamic information preferred embodiment obtained:
Test example 1: the pharmacodynamic study of natural drug composition treatment Chemotherapy-induced Diarrhea effect
1, test material
(1) Herba Andrographis, Radix Scutellariae, is purchased from medical material market.
(2) mice, body weight 20 ± 2g, male, ad lib drinking-water before experiment.
2, test method:
Mice is divided into 5 groups at random, and often organize 5, A group is model group, and B group is Herba Andrographis Radix Scutellariae water boiling concentration extract 10g.kg
-1(being equivalent to containing crude drug amount), C group is andrographolide 10g.kg
-1(being equivalent to containing crude drug amount), D group Radix Scutellariae total flavones mixes 10g.kg with baicalin in 3:4 ratio
-1(being equivalent to containing crude drug amount), E group is natural drug composition 10g.kg of the present invention
-1(being equivalent to containing crude drug amount), irinotecan intraperitoneal injection (75mg/kg), every day 1 time, continuous 4 days, copies Chemotherapy-induced Diarrhea model.Mice continuously by corresponding extract gavage 8 days, is placed in metabolic cage, spreads white filter paper to observe stool situation at the bottom of cage, and observe mice crissum situation and Mus tail fecal pollution situation by B, C, D, E group, in conjunction with cotton swab stimulation anus defecation, every day observed and recorded 2 times.Diarrhoea degree scale is as follows: 0 point: stool is normal or do not have; 1 point: laxativeness, stool is visible slight wet soft; 2 points: mild diarrhea, stool comparatively wets and not molding.And there is slight crissum painted; 3 points, severe is suffered from diarrhoea, and watery stool is also painted with severe crissum.
3, experimental result
Compare with model group, natural drug composition group Incidence of Diarrhea of the present invention obviously alleviates.The results are shown in Table 1.
Table 1 present composition is on mouse chemotherapy induced diarrhea incidence rate and scoring impact
Note: a is equivalent to containing crude drug amount
Test example 2: the pharmacodynamic study of natural drug composition antitumor action of the present invention
1, experiment material
(1) Kunming mouse, body weight 20 ± 2g, ad lib drinking-water before experiment, 24h natural light irradiation.
(2) cyclophosphamide, the permanent auspicious medical Pharmacy stock Co., Ltd in Jiangsu product.
2, test method:
To mice S
180the inhibition test of growing sarcoma
Employing is gone down to posterity 5-7 days, and well-grown milky ascites, is diluted to tumor cell suspension with appropriate physiological saline solution, and cell number is 10
7/ mL, often organizes 5, and every Mus right axillary fossa subcutaneous vaccination 0.2mL(is containing oncocyte 2 × 10
6individual).Inoculation random packet next day, natural drug composition of the present invention is divided into (200,100mg/mL) two dosage groups by experimental group, and every Mus gavage 0.5 ml, gives cyclophosphamide 10gkg simultaneously
-1d
-1; Negative control group gives 0.5 mL distilled water; Positive drug group gives cyclophosphamide 10gkg
-1d
-1, successive administration 10 days.Conventional raising, drinking-water, food is not limit.Drug withdrawal next day, mice all de-cervical vertebra is put to death, and strips subcutaneous solid tumors tumor block, weighs, by following formulae discovery tumor control rate (%):
Tumor control rate=(the average tumor of heavy (the C)-experimental group of the average tumor of matched group heavy (T)) average tumor of/matched group heavy (C)
3, result of the test
Natural drug composition of the present invention is low, high dose group all can strengthen cyclophosphamide agent with to S
180the effect of suppression degree, especially the strongest with high dose group effect.(as table 2)
Table 2 natural drug composition of the present invention is to S
180the tumor inhibition effect of mice
| Group | Suppression ratio (%) |
| Cyclophosphamide group | 31.5 |
| Compositions is low+cyclophosphamide | 36.9 |
| Height+cyclophosphamide | 44.7 |
Claims (2)
1. reduce a natural drug composition for cancer chemotherapy induced diarrhea, it is characterized in that said composition is made up of in the ratio of 5:6:6:8 by weight Herba Andrographis Radix Scutellariae water boiling concentration extract, andrographolide, Radix Scutellariae total flavones and baicalin,
Wherein the preparation method of Herba Andrographis Radix Scutellariae water boiling concentration extract is:
Be 1-4: 1 get Herba Andrographis, Radix Scutellariae, distilled water immersion 1h according to proportion compatibility, heating decocts 1h, and silk filters, medicinal residues add 6 times of distilled water again, soak, decoct, filter with method, by twice filtrate mixing, heating is concentrated, ultrafiltration membrance filter, 70 DEG C of drying under reduced pressure and get final product;
Wherein the preparation method of andrographolide is:
Take Common Andrographis Herd appropriate, enzymolysis pre-treatment, ethanol hot dipping, filter, recovery ethanol, obtain extractum, wash with petroleum ether, removing chlorophyll, water layer chloroform extraction is spent the night, is divided into water layer, chloroform layer and intermediate layer, by intermediate layer precipitate ethyl alcohol recrystallization, to obtain final product;
Wherein the preparation method of Radix Scutellariae total flavones is:
Take Radix Scutellariae coarse powder, 55% ethanol reflux, extract, at 85 DEG C repeatedly, is filtered, merging filtrate, reclaims ethanol, concentrated, dry, to obtain final product;
Wherein the preparation method of baicalin is:
Radix Scutellariae is dried, and pulverizes and sieves, and takes appropriate, with 80% ethanol with 10 times, 8 times amount extract at twice, each 1h, filter, merging filtrate, reclaims ethanol, adds appropriate distilled water, add concentrated hydrochloric acid and adjust pH3-4, elimination impurity after standing 1h, filtrate adjusts pH1.5-2 in 40 DEG C, insulation 30min, leaves standstill, sucking filtration, precipitation is washed to pH6.5-7.0, is drying to obtain.
2. natural drug composition as claimed in claim 1 reduces the purposes in cancer chemotherapy induced diarrhea medicine in preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310249494.2A CN103356741B (en) | 2013-06-23 | 2013-06-23 | Natural medicine composition |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310249494.2A CN103356741B (en) | 2013-06-23 | 2013-06-23 | Natural medicine composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103356741A CN103356741A (en) | 2013-10-23 |
| CN103356741B true CN103356741B (en) | 2015-05-13 |
Family
ID=49359543
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310249494.2A Expired - Fee Related CN103356741B (en) | 2013-06-23 | 2013-06-23 | Natural medicine composition |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103356741B (en) |
-
2013
- 2013-06-23 CN CN201310249494.2A patent/CN103356741B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 穿心莲内酯提取工艺研究;吴爱群等;《河池学院学报》;20091031;第29卷(第5期);第66页 * |
| 黄芩活性成分提取分离研究进展;曹岗等;《抗感染药学》;20090630;第6卷(第2期);第88页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103356741A (en) | 2013-10-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103191265A (en) | Traditional chinese medicine extract and preparation method thereof | |
| CN103191193A (en) | Traditional chinese medicine extract and preparation method thereof | |
| CN103191246A (en) | Traditional chinese medicine extract and preparation method thereof | |
| CN103191201A (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103202869A (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103202898B (en) | Traditional chinese medicine composition and preparation method thereof | |
| CN103251706A (en) | Traditional chinese medicine composition | |
| CN103301377A (en) | Natural pharmaceutical composition | |
| CN103340929A (en) | Natural drug extract | |
| CN103356741B (en) | Natural medicine composition | |
| CN103340970A (en) | Traditional chinese medicine composition | |
| CN103239529A (en) | Traditional chinese medicine composition | |
| CN103223028A (en) | Extract product of traditional Chinese medicine | |
| CN103316153A (en) | Natural pharmaceutical composition and preparation method thereof | |
| CN103222993A (en) | Chinese medicinal extract | |
| CN103223042A (en) | Traditional chinese medicine composition | |
| CN103263463A (en) | Traditional chinese medicine composition | |
| CN103223011A (en) | Extract product of traditional Chinese medicine | |
| CN103330751B (en) | Natural medicinal composition and preparation method and application thereof | |
| CN103316189A (en) | Natural pharmaceutical composition | |
| CN103393812A (en) | Traditional Chinese medicine composition and preparation method thereof | |
| CN103349697A (en) | Natural medicine extractive and preparation method and uses thereof | |
| CN103230427A (en) | Traditional Chinese medicine extract and preparation method thereof | |
| CN103263499A (en) | Traditional chinese medicine extract | |
| CN103191219A (en) | Traditional chinese medicine composition and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150513 Termination date: 20160623 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |