CN103344550A - Method for identifying silique shattering resistance of brassica napus - Google Patents
Method for identifying silique shattering resistance of brassica napus Download PDFInfo
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Abstract
The invention discloses a method for identifying silique shattering resistance of brassica napus. The method comprises: drying a silique at 80 DEG C for 30 min, sealing and reserving at room temperature overnight, putting into a column container in which 8 steel balls are placed; adopting an HQ45Z model shaking table, concussing the column container at a rotary speed of 280 rpm, observing every two minutes for 5 times; and repeating each material for 3 times, and using the formula that silique shattering index = Sigma Xi*(6-i)/silique number*total number to calculate the silique shattering index, while Xi represents the number of broken silique at the i time, 1<=i<=5, and the silique shattering resistance index = 1-silique shattering index. The method is good in operationality, high in accuracy, good in repeatability and high in identification efficiency; and the method helps to effectively discriminate the difference of silique shattering resistance of the present rape varieties, is suitable for popularization and application in screening and identification of rape silique-shattering-resistant variety and heredity research of rape silique shattering resistance, and helps to provide a technology foundation for seed selection and heredity research of rape silique-shattering-resistant variety.
Description
Technical field
The invention belongs to property authenticate technology field, cabbage type rape cracking resistance angle, relate in particular to a kind of method of identifying cabbage type rape cracking resistance angle property.
Background technology
In crop domestication process, for preventing crop failure, human initiatively the selection reduced the kind of splitting the corner grain, makes crop be more suitable for agricultural production.All exist as rape, soybean, the sesame in main edible oil starting material source and to split the angle problem, and rape is even more serious.Can be divided into and initiatively split angle and passive two kinds at the angle of splitting splitting the angle according to splitting the angle process, initiatively splitting the angle is after the fruit maturation of angle, the cell dehydration, produce tension force between the cell, cell is degraded under the effect of enzyme, tension force between the cell impels the angle pericarp to separate with middle replum and splits the angle, as the cracking of Fructus Trichosanthis anguinae fruit.Passive splitting in the process of angle when cell dehydration or degraded, the tension force deficiency between the cell needs by the effect of external force really split in the angle, as the cracking of siliqua of oilseed rape so that the angle pericarp separates with middle replum.Split for the angle for passive, estimating Different Individual, to split the key of angle property difference be the standard method of setting up a kind of accurate evaluation cracking resistance angle property.Cracking resistance angle property authentication method is carried out research widely both at home and abroad, but do not found the high method of a kind of strong operability and accuracy all the time.
In the process of carrying out the property evaluation of rape cracking resistance angle, we once attempted multiple authentication method.Split the angle resistance with reference to what the baking seasoning of soybean was identified rape, but change water cut or the stoving time of angle fruit howsoever, all can't make it cracking.The abscission zone structure of report such as Christiansen soybean and the arabidopsis structural similarity of Cruciferae, rape abscission zone structure but has remarkable difference.What form between the abscission zone of siliqua of oilseed rape and the false dissepiment is V-structure, and being connected between cell and the cell is tightr, and transition is milder between the abscission zone of the angle of soybean and arabidopsis fruit and the false dissepiment, the easier angle of splitting.Seed holding authentication method with reference to paddy rice adopts the Free Falling Body in Upper Air method to identify the cracking resistance angle property of 140 parts of rape strains, the result test test material the rate of not splitting between 64.8%-96.6%, the rate of wherein not splitting has 51 parts greater than 90% material, between 80% to 90%, there are 83 parts, institute's value is too concentrated, storeroom difference is not remarkable, may be fruit give birth to the mode difference and cause.Paddy rice is mainly with a bunch arrangement, and siliqua of oilseed rape disperses on plant, when using the high-altitude method of landing to identify, because gravity is not enough, and the angle fruit limited amount that rape breaks.The field nature shattering method of utilizing the India scholar to propose is carried out rape when splitting the angle resistance and identifying, the cracking situation of angle fruit on the ripe 4-5 week " Invest, Then Investigate " of rape plant, because the bird pest in later stage in rape maturity stage, angle fruit great majority on the field plant all ftracture, serious lodging after most of material maturation is difficult to accurately investigation simultaneously.
Tension method is to improve according to the drawing crack method, use easy to carry, the easy fixedly tautness meter of installation instead, angle fruit carpopodium is placed on the drag hook, firmly press the angle angle pericarp is separated with false dissepiment, dynamometer records disrupture force automatically, and result's stability and reliability are improved.The result who utilizes tension method to identify is remarkable with the results relevance of random collision method.But in qualification process, angle fruit fixedly be a difficult problem, the junction of carpopodium and drag hook is difficult for grasping, the personal error of qualification result strengthens, the result between the different experiments chamber is difficult for comparison.Because this method identifies that speed is fast, the efficient height, equipment is simple, cheap, can be used for the quick preliminary screening of a large amount of breeding materials.
Random collision method simulation angle fruit more can be reflected the angle process of splitting in field and the extraneous situation that contacts.Wen Yancheng etc. with this method improvement after, qualification result is easier quantitatively, accuracy also is improved.But the shaking table of its used turn up 400rpm, amplitude 24mm is difficult to buy in China on the market, the like product price of customization or import is on 100,000 yuan, utilize simultaneously under 20 ℃, relative humidity 50% condition, to place and carry out water balance 2 weeks lot of materials is difficult to operation, an angle of rupture fruit of one minute record of every vibration number also makes identifies that required time is longer, complex steps.
Summary of the invention
The invention provides a kind of method of identifying cabbage type rape cracking resistance angle property, be intended to solve the method for present evaluation cabbage type rape cracking resistance angle property, accuracy and the evaluation efficient of length, complex steps, qualification result is lower to identify required time, can not distinguish the problem of present rape variety cracking resistance angle property difference effectively.
The object of the present invention is to provide a kind of method of identifying cabbage type rape cracking resistance angle property, this method may further comprise the steps:
Further, in step 1, the angle fruit is carried out water balance at 80 ℃ of oven dry 30min, the room temperature sealing is preserved back overnight and is identified.
Further, in step 1, in cylindrical chamber, place 20 angle to be identified fruits.
Further, in step 1, the internal diameter of cylindrical chamber is 14.8cm, the high 7.4cm of being.
Further, in step 1, the diameter of the steel ball of placing is 14mm in the cylindrical chamber.
Further, in step 2, model can reach the rotating speed of 280rpm in 5 seconds for the HQ45Z shaking table.
Further, in step 2, observed torn corners fruit number one time, and observed altogether 5 times in per two minutes.
Further, in step 3, utilize formula cracking resistance angle index=1-to split the angle index, split angle index=∑ Xi * (6-i)/angle fruit number * total degree, calculate cracking resistance angle exponential sum and split the angle index, Xi is really number of the i time damaged angle, 1≤i≤5.
The method of evaluation cabbage type rape cracking resistance provided by the invention angle property at 80 ℃ of oven dry 30min, after room temperature sealing preservation is overnight, is put into cylindrical chamber with the angle fruit, places 8 steel balls in the cylindrical chamber; Adopting model is the HQ45Z shaking table, with the 280rpm rotating speed cylindrical chamber is shaken processing, observed once in per two minutes, observe altogether 5 times, every part of material repeats 3 times, utilizes formula: split angle index=∑ Xi * (6-i)/angle fruit number * total degree and calculate and split the angle index, Xi is really number of the i time damaged angle, 1≤i≤5, cracking resistance angle index=1-splits the angle index; This method strong operability, accuracy height, good reproducibility, evaluation efficient height, can distinguish the difference of present rape variety cracking resistance angle property effectively, be adapted at applying in the research of the Screening and Identification of rape cracking resistance angle property kind and rape cracking resistance angle sex-controlled inheritance, for seed selection and the genetic research of rape cracking resistance angle property kind provides technical foundation, practical, have stronger popularization and using value.
Description of drawings
Fig. 1 is the realization flow figure of the method for the evaluation cabbage type rape cracking resistance angle property that provides of the embodiment of the invention;
Fig. 2 is that the water balance that provides of the embodiment of the invention is to identifying the synoptic diagram that influences of fruit cracking resistance angle, angle index;
Fig. 3 is that the shaking table performance that provides of the embodiment of the invention is to identifying angle fruit cracking resistance angle index result's the synoptic diagram that influences;
Fig. 4 is the synoptic diagram that the different recording modes of the employing that provides of the embodiment of the invention are identified cracking resistance angle index result;
Fig. 5 is the cracking resistance angle exponential distribution histogram that the different steel ball numbers of the employing that provides of the embodiment of the invention are identified 39 strains;
Fig. 6 is the histogram as a result that 103 kinds to participating in the rape variety regional testing in 2010 that the embodiment of the invention provides are identified.
Embodiment
In order to make purpose of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is further described in detail.Should be appreciated that specific embodiment described herein only in order to explaining the present invention, and be not used in and limit invention.
The realization flow of the method for the evaluation cabbage type rape cracking resistance angle property that Fig. 1 provides for the embodiment of the invention.
This method may further comprise the steps:
In step S101, the angle fruit at 80 ℃ of oven dry 30min, after room temperature sealing preservation is overnight, is put into cylindrical chamber, place 8 steel balls in the cylindrical chamber; Angle fruit carries out water balance through 80 ℃, 30min baking,
In step S102, adopting model is the HQ45Z shaking table, with the 280rpm rotating speed cylindrical chamber is shaken processing, observes once in per two minutes, observes altogether 5 times, and every part of material repeats 3 times;
In step S103, utilize formula: split angle index=∑ Xi * (6-i)/angle fruit number * total degree and calculate and split the angle index, Xi is really number of the i time damaged angle, 1≤i≤5, and cracking resistance angle index=1-splits the angle index.
In embodiments of the present invention, in step S101, the angle fruit is carried out water balance at 80 ℃ of oven dry 30min, the room temperature sealing is preserved back overnight and is identified.
In embodiments of the present invention, in step S101, can in cylindrical chamber, place 20 angle fruits.
In embodiments of the present invention, in step S101, the internal diameter of described cylindrical chamber is 14.8cm, the high 7.4cm of being.
In embodiments of the present invention, in step S101, the diameter of the steel ball of placing is 14mm in the cylindrical chamber.
In embodiments of the present invention, in step S102, model can reach the rotating speed of 280rpm in 5 seconds for the HQ45Z shaking table.
In embodiments of the present invention, in step S102, observed torn corners fruit number one time, and observed altogether 5 times in per two minutes.
In embodiments of the present invention, in step S103, utilize formula cracking resistance angle index=1-to split the angle index, split angle index=∑ Xi * (6-i)/angle fruit number * total degree, calculate cracking resistance angle exponential sum and split the angle index, Xi is really number of the i time damaged angle, 1≤i≤5.
Below in conjunction with drawings and the specific embodiments application principle of the present invention is further described.
The optimization of random collision method
Water balance is to the influence of qualification result:
The water cut of angle fruit is bigger to the influence of cracking resistance angle property qualification result, for improving accuracy of experimental results, guarantee test material water cut unanimity, adopt the random collision method to identify before to material unification dry processing, to inquire into water balance to the influence of qualification result.
By the stoving time test of baking 10-60min, a processing of every 10min is set, after 80 ℃ of baking 30min were really put at the angle of discovery different cultivars, the angle fruit of all kinds was no longer weightless, and fully drying of angle fruit is described, moisture has reached zero.Satisfying baking condition and be set to 80 ℃, 30min, really is contrast with the angle of not toasting.Find in the experimentation to identify that immediately cracking resistance angle property declines by a big margin after the baking, consider baking back higher angular fruit Temperature Influence, angle after baking fruit is provided with two kinds of processing modes again, namely identifies immediately and in freshness protection package, seal and identify after room temperature preservation is spent the night.
At first compared the result that room temperature preservation back overnight, baking back is identified and identified immediately.The average cracking resistance angle index of identifying immediately after 60 parts of materials of participating in the experiment toast is 0.033, and range of variation is 0-0.233; The average cracking resistance angle index of identifying after overnight is 0.157, and range of variation is 0-0.703, and two kinds of related coefficients of handling qualification result are 0.7661 (P=2.55E
-07), correlation it very is remarkable.But the cracking resistance angle index of identifying immediately after the baking is less than normal, and most materials (are accounting for 91.7%) below 0.1, are difficult to reflect the difference of storeroom cracking resistance angle property.And the cracking resistance angle exponential distribution of baking back evaluation overnight is more reasonable,＜0.1,0.1-0.2,0.2-0.3,0.3-0.4,0.4-0.5,＞have 29,16,6,3,3 and 3 respectively in 0.5 the scope, can reflect the difference between different materials.
Choose the comparison test that 22 parts of materials toast and do not toast processing in addition, baking is handled and is adopted the evaluation overnight of baking back room temperature preservation.The average cracking resistance angle index that does not toast processing is 0.341, and range of variation is 0.143-0.575; The average cracking resistance angle index that baking is handled is 0.392, and range of variation is 0.118-0.818 (Fig. 2).Correlation analysis shows that the related coefficient of two kinds of method qualification results is 0.7641 (P=0.0027), and correlation it very is remarkable, two kinds of processing are described after qualification result more consistent.But the range of variation of qualification result is bigger after the baking, can reflect the difference between the expert evidence more realistically, and the difference between the different repetitions of the same material as a result of baking back evaluation is littler, has reduced test error, has improved the accuracy of qualification result.
The shaking table performance is to the influence of qualification result:
The shaking table performance index comprises maximum (top) speed, amplitude of vibration, lifting degree of hastening etc.The THZ-C type constant temperature shaking table (calling old shaking table in the following text) for magnificent biochemical instrument factory that this test is selected for use at first, its lifting degree of hastening is slower, rotating speed reaches that to set the needed time of rotating speed longer, and rotating speed rises to 280 rev/mins sometimes even needs about 20 seconds, and not between homogeneous lifting speed take non-constant.In order to select the shaking table of stable performance, determined the shaking table that a raising speed is very fast, degree of accuracy is higher (HQ45Z of section in the Wuhan calls new shaking table in the following text), can in 5 seconds, reach the setting rotating speed.
Select for use 39 parts of materials to compare the difference of new and old two shaking table qualification results.The cracking resistance angle index range of variation that adopts old shaking table to identify is 0.003-0.703, and mean value is 0.168.The cracking resistance angle index range of variation that new shaking table is identified is 0.000-0.615, and mean value is 0.163 (Fig. 3).The cracking resistance angle exponential average of shaking table evaluation newly is littler than old shaking table evaluation, and reason may be that angle fruit rotating speed collision time with setting in new shaking table is longer relatively.But analyse in depth the cracking resistance angle property qualification result of each part material and repeat differences, be not difficult to find that the old shaking table qualification result coefficient of variation is bigger than normal.The result that two different shaking tables are identified carries out correlation analysis, and its related coefficient is 0.9714 (P=2.261E
-47), relevant pole is remarkable, shows that although the cracking resistance angle property result who adopts the shaking table of different performance to identify is variant, the trend comparison unanimity has certain repeatability when using the random collision method to identify.
The concussion number of times is to the influence of qualification result:
Use the random collision method to identify, the angle fruit number that every concussion 1min record breaks records 10 times altogether, and the time of cost is longer, and process is loaded down with trivial details.Identify efficient for improving, use an angle of rupture fruit of every concussion 2min record number instead, record altogether 5 times, and the angle formula of index is split in modification accordingly, (Xi is really number of the i time damaged angle namely to split angle index=∑ Xi * (6-i)/angle fruit number * total degree, 1≤i≤5), cracking resistance angle index=1-splits the angle index.Be prerequisite with identical shaking table with identical rotating speed, select for use 28 kinds (being) to compare reliability and the correlativity of two kinds of recording method qualification results.The result shows, every concussion 1min record once (RIT-1) the 28 parts of average cracking resistance of material angle indexes identifying is 0.423, and maximal value is 0.818, and minimum value is 0.118, and the difference of maximal value and minimum value is 0.7.Every concussion 2min record once (RIT-2) average cracking resistance angle index of identifying is 0.345, and maximal value is 0.750, and minimum value is 0.020, and the difference of maximal value and minimum value is 0.73 (Fig. 4).The related coefficient of two kinds of recording mode qualification results is 0.6985 (P=0.00117), and relevant pole is remarkable, and the trend unanimity of two kinds of recording mode qualification results is described.The cracking resistance angle exponential average that RIT-2 obtains diminishes, but the storeroom variation amplitude strengthens, and more can embody the difference of the cracking resistance angle property of storeroom.
Different steel ball numbers are to the influence of qualification result:
Select and shake time-optimizedly by water balance, shaking table, set up basically and simplified and random collision method reliably.But when measuring a large amount of sample, find that the cracking resistance angle property of most of material is on the low side, nearly 1/4th kind (being) cracking resistance angle index is zero, is difficult to tested variety (being) is distinguished.In order to increase the difference of storeroom, improving the water balance method, stablizing under the prerequisite of shaking table performance and collision time, attempt improving cracking resistance angle index by reducing collision steel ball number.Choose the different 39 parts of kinds (being) of cracking resistance angle property and carry out different steel ball numbers to the test of qualification result influence, 8 and two processing of 10 steel balls are set.The result shows, utilize 8 steel balls to identify that the average cracking resistance angle index of 39 kinds (being) is 0.188, variation amplitude is 0.003-0.793,＜0.1,0.1-0.2,0.2-0.3,0.3-0.4,0.4-0.5,0.5-0.6 and＞0.6 have 17,9,5,3,2,1 and 2 respectively; And the average cracking resistance angle index that utilizes 10 steel balls to identify is 0.168, and variation amplitude is 0-0.567,＜0.1,0.1-0.2,0.2-0.3,0.3-0.4,0.4-0.5,0.5-0.6 and＞0.6 have respectively 17,9,8,1,2,2 and 0 (Fig. 5).The related coefficient of two kinds of method qualification results is 0.9198 (P=1.03
E-06), relevant pole is remarkable.But two kinds of coefficient of variation differences of handling qualification result between repetition, the coefficient of variation between 10 steel ball qualification results repeat is 0.046, the qualification result (0.035) that is higher than 8 steel balls, the qualification result repeatability that 8 steel balls are described is better, and the material that cracking resistance angle index is bigger in 8 steel ball qualification results is more relatively, the range of variation of cracking resistance angle index is bigger, more can embody the real difference of cracking resistance angle property between different materials.
The effect of improvement random collision method:
Use the random collision method after optimizing, namely drying 30min through 80 ℃, seal 20 angles after the placement overnight and really put into the cylindrical chamber of internal diameter 14.8cm, high 7.4cm, place 8 steel balls that diameter is 14mm in the container, adopt HQ45Z (section in the Wuhan) shaking table, shake with 280rpm, observed once in per two minutes, observe altogether 5 times, every part of material repeats 3 times, utilize formula: split angle index=∑ Xi * (6-i)/angle fruit number * total degree (Xi is the i time damaged angle fruit number, 1≤i≤5) calculating and split the angle index, cracking resistance angle index=1-splits the angle index.2010 are participated in 103 kinds (being) of rape variety regional testing and identify, the result shows that the average cracking resistance angle index of participating in 103 cabbage type rape varieties (being) of rape regional testing in 2010 is 0.148, and range of variation is 0-0.790.Wherein cracking resistance angle index has 53 parts in the kind (being) of 0-0.1, accounts for 51.46% of sum; Kind (being) at 0.1-0.2 has 24 parts, accounts for 23.3% of sum; Kind (being) at 0.2-0.3 has 11 parts, accounts for 10.68% of sum; 0.3-0.4 kind (being) have 8 parts, account for the sum 7.77%; Kind (being) at 0.4-0.5 has 3 parts, accounts for 2.9% of sum; Being higher than 0.5 kind (being) has 4 parts, accounts for 3.88% (Fig. 6) of all material.The cracking resistance angle index of kind over half (being) is all between 0-0.1, and illustrating that most of rape strain of newly breeding at present all belongs to easily splits the angle section material.
When the property of the cracking resistance angle of use random collision method evaluation rape, find that shaking table performance, collision steel ball number, angle fruit water cut etc. influence bigger to qualification result.Utilize laboratory common shaking table (old shaking table at first, market price 2000-3000 unit/platform), although shaking table has been set to maximum (top) speed (280rpm), because time of vibration is short, just one minute at every turn, lifting speed temporal differences (10-20 second) did not make that error strengthens between repetition between homogeneous.New shaking table (market price 6000-7000 unit/platform) reaches maximal rate only needed for 5 seconds, and not basic identical between homogeneous, had reduced the test error between sample repeats, and also by the water cut of having tested clearly the angle fruit cracking resistance angle index was had appreciable impact recently.Because it is that interaction because of the tension force between the cell and external force after the cell dehydration produces that rape splits the angle, utilize baking to handle to cause the abundant drying of angle fruit to be the method for best equilibrium moisture, namely simple and easy to do, can guarantee the consistance of storeroom again.Find in the baking Processing Test process to identify that immediately the cracking resistance angle index of material is less than normal after the baking that reason may be that the thermal motion of molecule in the fruit of angle is accelerated and iuntercellular tension force is strengthened, and impels the easier cracking of angle fruit.Because cooling rate is subjected to the influence of room temperature, for reducing test error, the angle after the baking really needs at room temperature to seal preservation, fully identifies after the cooling again.This research select by water balance, shaking table and shake time-optimized after, when finding to measure a large amount of sample, the cracking resistance angle property of material is on the low side, is difficult to distinguish resistance difference, the especially resistance material preferably of material of participating in the experiment, and can not suitably space out.After will colliding the steel ball number and being reduced to 8 by 10, the resistance difference of storeroom is strengthened, drawn back the distance of storeroom better.Test in all contrast processing at this, no matter utilize tension method or random collision method, baking still be identify immediately after not toasting, toasting still be evaluation overnight, new shaking table still be old shaking table, one minute record once still be recorded once in two minutes, 8 still be 10 collide steel ball, the correlativity of the qualification result that obtains all reaches the utmost point level of signifiance, illustrates that the trend comparison of cracking resistance angle property difference of rape variety is constant.The improvement of details and optimization in the method, can job change the trend of performance, but can make quantitative qualification result more accurate.
It is 0-0.790 that random collision method after the utilization improvement identifies the cracking resistance angle index range of variation of participating in 103 cabbage type rape varieties (being) of rape regional testing in 2010.Use random collision methods such as Wen Yancheng have been identified 229 parts of cabbage type rape strains, and its cracking resistance angle index variation amplitude is 0.000-0.7675.50 parts of cabbage type rape strains that Peng Pengfei etc. utilize the method identical with Wen Yancheng etc. to identify to participate in 2008 Hubei Province and rape variety district, middle reaches district, national the Changjiang river to try, its cracking resistance angle index luffing is 0.020-0.470.Comparatively speaking, the qualification result of the random collision method after this improvement not only accuracy increases, and more helps to differentiate the difference of storeroom.The authentication method of the present invention's and reliable results comparatively simple as, the fairly simple cheapness of evaluation apparatus, simple to operate, criterion is clear and definite, conventional breeding and research unit can both use, be fit to cracking resistance angle property evaluation that accuracy is had relatively high expectations, as genetic analysis, QTL location, the key material of breeding and parent's evaluation etc.
Optimize the cracking resistance angle property authenticate technology of rape variety (being), set up a cover strong operability, authentication method that accuracy is high, it is the basis of seed selection cracking resistance angle rape variety, this invention is optimized some parameters of random collision method, be tested and appraised a collection of rape variety (being), verified the applicability of the random collision method after improving, for seed selection and the genetic research of rape cracking resistance angle property kind provides technical foundation.
The method of the evaluation cabbage type rape cracking resistance angle property that the embodiment of the invention provides at 80 ℃ of oven dry 30min, after room temperature sealing preservation is overnight, is put into cylindrical chamber with the angle fruit, places 8 steel balls in the cylindrical chamber; Adopting model is the HQ45Z shaking table, with the 280rpm rotating speed cylindrical chamber is shaken processing, observed once in per two minutes, observe altogether 5 times, every part of material repeats 3 times, utilizes formula: split angle index=∑ Xi * (6-i)/angle fruit number * total degree and calculate and split the angle index, Xi is really number of the i time damaged angle, 1≤i≤5, cracking resistance angle index=1-splits the angle index; This method strong operability, accuracy height, good reproducibility, evaluation efficient height, can distinguish the difference of present rape variety cracking resistance angle property effectively, be adapted at applying in the research of the Screening and Identification of rape cracking resistance angle property kind and rape cracking resistance angle sex-controlled inheritance, for seed selection and the genetic research of rape cracking resistance angle property kind provides technical foundation, practical, have stronger popularization and using value.
The above only is preferred embodiment of the present invention, not in order to limiting the present invention, all any modifications of doing within the spirit and principles in the present invention, is equal to and replaces and improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. method of identifying cabbage type rape cracking resistance angle property is characterized in that this method may further comprise the steps:
Step 1 at 80 ℃ of oven dry 30min, after room temperature sealing preservation is overnight, is put into cylindrical chamber with the angle fruit, places 8 steel balls in the cylindrical chamber;
Step 2, adopting model is the HQ45Z shaking table, with the 280rpm rotating speed cylindrical chamber is shaken processing, observes torn corners fruit number one time in per two minutes, observes altogether 5 times, every part of material repeats 3 times;
Step 3, utilize formula: split angle index=∑ Xi * (6-i)/angle fruit number * total degree and calculate and split the angle index, Xi is really number of the i time damaged angle, 1≤i≤5, cracking resistance angle index=1-splits the angle index.
2. the method for evaluation cabbage type rape cracking resistance as claimed in claim 1 angle property is characterized in that, in step 1, the angle fruit is carried out water balance at 80 ℃ of oven dry 30min, and the room temperature sealing is preserved back overnight and identified.
3. the method for evaluation cabbage type rape cracking resistance as claimed in claim 1 angle property is characterized in that, in step 1, places 20 angles to be identified fruits in cylindrical chamber.
4. the method for claim 1 is characterized in that, in step 1, the internal diameter of cylindrical chamber is 14.8cm, the high 7.4cm of being.
5. the method for evaluation cabbage type rape cracking resistance as claimed in claim 1 angle property is characterized in that in step 1, the diameter of the steel ball of placing is 14mm in the cylindrical chamber.
6. the method for evaluation cabbage type rape cracking resistance as claimed in claim 1 angle property is characterized in that in step 2, model can reach the rotating speed of 280rpm in 5 seconds for the HQ45Z shaking table.
7. the method for evaluation cabbage type rape cracking resistance as claimed in claim 1 angle property is characterized in that, in step 2, observes torn corners fruit number one time, and observes altogether 5 times in per two minutes.
8. the method for evaluation cabbage type rape cracking resistance as claimed in claim 1 angle property, it is characterized in that, in step 3, utilize formula cracking resistance angle index=1-to split the angle index, split angle index=∑ Xi * (6-i)/angle fruit number * total degree, calculate cracking resistance angle exponential sum and split the angle index, Xi is the i time damaged angle fruit number, 1≤i≤5.
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| CN109900631A (en) * | 2019-02-25 | 2019-06-18 | 中国农业科学院油料作物研究所 | A method of identification sesame cracking resistance capsule |
| CN112268991A (en) * | 2020-11-03 | 2021-01-26 | 重庆市农业科学院 | Method for rapidly detecting pod shattering resistance of rape pod |
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| CN104109682A (en) * | 2014-07-11 | 2014-10-22 | 中国农业科学院油料作物研究所 | Pectate lyase BnPL gene as well as promoter and application thereof |
| CN104109682B (en) * | 2014-07-11 | 2016-09-07 | 中国农业科学院油料作物研究所 | A kind of pectin lyase BnPL gene and promoter thereof and application |
| CN109900631A (en) * | 2019-02-25 | 2019-06-18 | 中国农业科学院油料作物研究所 | A method of identification sesame cracking resistance capsule |
| CN109900631B (en) * | 2019-02-25 | 2021-06-29 | 中国农业科学院油料作物研究所 | Method for identifying sesame cracking resistance capsules |
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