CN103336010A - Early tobacco virus disease detection method based on infrared thermal imaging technology - Google Patents
Early tobacco virus disease detection method based on infrared thermal imaging technology Download PDFInfo
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Abstract
The invention discloses an early tobacco virus disease detection method based on an infrared thermal imaging technology, which comprises the steps that a tobacco infrared thermal image is subjected to image intensification from image smoothing, image sharpening and histogram equalization; a new tobacco leaf thermal image area and an old tobacco leaf thermal image area are extracted by a threshold segmentation method; if a temperature of a growth environment of tobacco is higher than 16 DEG C and less than or equal to 28 DEG C, a difference value between temperature values of various pixel points in the new tobacco leaf area and an average value of the temperature values is calculated; when an absolute value of the difference value is greater than 1 DEG C, a plant is a disease plant; if not, the plant is not the disease plant; when the environment temperature is higher than 28 DEG C and less than 34 DEG C, a difference value between average temperatures of the pixels in the new tobacco leaf area and the old tobacco leaf area is calculated; when the difference value is less than 0.8 DEG C, the plant is the disease plant; and if not, the plant is not the disease plant. The method is simple in step, short in consumed time and high in accuracy, can detect a disease earlier, can be applied to long-distance real-time monitoring of a field crop, and provides timely and reliable information of a crop growth condition, a disease condition and the like.
Description
Technical field
The present invention relates to a kind of early detection method of tobacco virus, refer in particular to and utilize infrared thermal imaging technique that tobacco virus is carried out the method for early detection, belong to the Non-Destructive Testing field.
Background technology
Tobacco is a kind of important industrial crops, is the important source of finance of country and local property tax, is subjected to the great attention of relevant department.In recent years, tobacco virus successively endangered in countries in the world, and outbreak of epidemic often, had caused tremendous loss, the enthusiasm of directly having dampened the tobacco grower to tobacco production.In China, cause generation area and the extent of injury of tobacco virus obviously to strengthen, its field diseased plant rate is generally at 20%-40%, heavy reaches 40%-80%, the plot that has even total crop failure.After tobacco infects virus, leaf growth is suppressed, leaf is little, lopsided, and underproduction amplitude can reach 20%-80%, simultaneously, sick leaf irregular colour after modulating is even, jealous relatively poor, quality descends, and does not also have a kind of medicament that result for the treatment of is comparatively desirable at present, a virosis disaster out of control is enough to destroy a cigarette district, so disease plant is accomplished early diagnosis and detect in advance to seem particularly important.
At present, in tobacco virus detects, relatively more commonly used is immunological methods such as enzyme linked immunological adsorption reaction, quick detection test paper bar, that this method has is highly sensitive, high specificity and advantage such as easy and simple to handle, but it has its limitation and deficiency---various tobacco viruses have different physicochemical properties, morphological character and antigenic property, the preparation of antibody producing, mensuration and enzyme labeling thing also just can not standardization, in addition, also can be subjected to specificity, nonspecific interference during detection.In the actual plantation, the tobacco grower is by virtue of experience, judges disease according to sick leaf reveal any symptoms, i.e. artificial sense assessment method, this method subjectivity is strong, poor reliability, and often based on the plant performance significantly, typical disease symptom, for the time evening.
PI viral disease detection technique exist step lengthy and tedious, breakage arranged, be unsuitable for the deficiency of aspects such as the extensive detection in field.Infrared thermal imaging technique is to utilize object each several part temperature contrast to cause the infrared emanation difference, and the sightless heat radiation situation of object is converted to visual thermography, thus the technology of acquired information.Because it has hypersensitivity to temperature, be a kind of noncontact, do not destroy, in real time, method for quick, can detect the temperature variation less than 0.1 ℃, this technology maturation is applied to fields such as electric, aviation and medical science, application in the plant disease early detection is a new developing direction, also will become a new research focus.This technology is particularly suitable for the transpiration of remote monitoring plant, be expected to the large tracts of land tobacco plant growing state, have or not aspects such as disease and pest and output estimation that reliably and timely information is provided.
Summary of the invention
The object of the present invention is to provide a kind of tobacco virus early detection method based on infrared thermal imaging technique, realize noncontact, do not destroy, in real time, the extensive tobacco virus in fast detecting field.
In order to solve above technical matters, the concrete technical scheme that the present invention adopts is as follows:
A kind of tobacco virus early detection method based on infrared thermal imaging technique is characterized in that may further comprise the steps:
Step 1, set-up procedure: choose the tobacco plant n strain of plant height, leaf age and growing way unanimity, n is even number, and n 〉=50 are divided into two groups of control group and inoculation group, and control group is smeared the 0.02M phosphate buffer, inoculation group is smeared tobacco susceptible virus, and is labelled, carries out record;
Step 2, measuring process: utilize the infrared thermography that is fixed on the tripod to take, camera lens during storage, is write down the number storing of every blade apart from tobacco plant 35-55cm, copies photo to computing machine again;
Step 3, data handling procedure: computing machine carries out Treatment Analysis to the tobacco thermal imagery, the identification disease plant.
The described thermal imagery processing procedure that the tobacco thermal imagery is carried out Treatment Analysis is specially:
Thermal imagery is carried out the figure image intensifying from image smoothing, image sharpening, three aspects of histogram equalization to the tobacco infrared chart respectively, adopt threshold segmentation method to extract young leaves and Lao Ye thermal imagery zone;
The tobacco growing environment temperature is higher than 16 ℃ and be lower than when equaling 28 ℃, and temperature jump appears in disease plant young leaves thermal imagery zone, and disease plant young leaves thermal imagery regional temperature changes evenly;
The tobacco growing environment temperature is higher than 28 ℃ and when being lower than 34 ℃, disease plant young leaves thermal imagery regional temperature changes evenly, and consistent with Lao Ye thermal imagery regional temperature, is high-temperature area, and disease plant young leaves thermal imagery zone is not low-temperature region, and Lao Ye thermal imagery zone is high-temperature area.
The method of discrimination of described identification disease plant is as follows:
When the tobacco growing environment temperature is higher than 16 ℃ and be lower than when equaling 28 ℃, calculate the temperature value of each pixel in the young leaves zone and the difference of its mean value, when the absolute value of difference greater than 1 ℃, then be disease plant, otherwise, be disease plant not;
When the tobacco growing environment temperature is higher than 28 ℃ and when being lower than 34 ℃, calculate in the young leaves zone and the medial temperature difference of pixel in the Lao Ye zone, when difference is lower than 0.8 ℃, then be disease plant, otherwise, then be disease plant not.
Described tobacco susceptible virus can be the mixed liquor of tobacco mosaic virus (TMV) TMV, marmor upsilon PVY and cucumber mosaic virus CMV.
The present invention has beneficial effect.The present invention adopts infrared thermal imaging technique, utilizes object each several part temperature contrast to cause the infrared emanation difference, and the sightless heat radiation situation of object is converted to visual thermography, and temperature is had hypersensitivity.Compare with current widely used biological method, step is simple, weak point consuming time; Compare with manual method, objectivity is strong, and the accuracy height the most important thing is, can more early detect disease; Can carry out the remote real-time monitoring field crop, for crop growth conditions, the timely exact information that provides such as disease-free is arranged.
Embodiment
The present invention is described in further detail below by specific embodiment.
Embodiment 1
1. choose tobacco plant 60 strains of plant height, leaf age and growing way unanimity, bottom Lao Ye is cut in every strain, stays 4 leaves in top, and 30 strains are that control group, 30 strains are inoculation group.0.02mol/L phosphate buffer with 10 times of volumes (V/W), pH 7.0, grind the fresh sick leaf that infects TMV, PVY and CMV3 kind virus, get supernatant after the filtered through gauze, to tobacco several the 4th leaves inoculations from top to bottom, control group is smeared the 0.02mol/L phosphate buffer with the frictional inoculation method of routine, and pH 7.0, labelled, carry out record.Two groups of plant branches are opened in the hot-house culture of plant illumination culturing rack, light intensity 10000 luxs, 18 ± 2 ℃ of temperature, humidity 70 ± 10%.
The morning 9:00, tobacco plant is put in balance 60min in the measurement environment, from inoculating preceding 1 day to inoculating back 7 day continuous acquisition thermal imaging every day figure.Infrared thermography is fixed on the tripod photographs, and camera lens during storage, is write down the number storing of every blade apart from tobacco plant 35-55cm, and is last, copies photo to computing machine.
3. data are handled, from image smoothing, image sharpening, three aspects of histogram equalization the tobacco infrared chart is carried out the figure image intensifying respectively, adopt threshold segmentation method to extract young leaves thermal imagery zone, calculate the temperature value of each pixel in susceptible group and the not susceptible group of young leaves zone and the difference of its mean value, for disease plant, the absolute value of difference is 1.0-1.3 ℃, and for disease plant not, the absolute value of difference is 0.6-0.8 ℃.
Embodiment 2
1. choose tobacco plant 50 strains of plant height, leaf age and growing way unanimity, bottom Lao Ye is cut in every strain, stays 4 leaves in top, and 25 strains are that control group, 25 strains are inoculation group.0.02mol/L phosphate buffer with 10 times of volumes (V/W), pH 7.0, grind the fresh sick leaf that infects TMV, PVY and CMV3 kind virus, get supernatant after the filtered through gauze, to tobacco several the 4th leaf inoculations from top to bottom, control group is smeared the 0.02mol/L phosphate buffer with conventional frictional inoculation method, pH 7.0,, labelled, carry out record.Two groups of plant branches are opened in the hot-house culture of plant illumination culturing rack, light intensity 10000 luxs, 32 ± 2 ℃ of temperature, humidity 70 ± 10%.
The morning 9:00, tobacco plant is put in balance 60min in the measurement environment, from inoculating preceding 1 day to inoculating back 7 day continuous acquisition thermal imaging every day figure.Infrared thermography is fixed on the tripod photographs, and camera lens during storage, is write down the number storing of every blade apart from tobacco plant 35-55cm, and is last, copies photo to computing machine.
3. data are handled, from image smoothing, image sharpening, three aspects of histogram equalization the tobacco infrared chart is carried out the figure image intensifying respectively, adopt threshold segmentation method to extract young leaves and Lao Ye thermal imagery zone, calculate disease plant and the young leaves zone of disease plant not and the medial temperature difference of Lao Ye area pixel, for disease plant, temperature gap is 0.5-0.6 ℃, and for disease plant not, temperature gap is 1.2-1.3 ℃
Embodiment 3
1. choose tobacco plant 70 strains of plant height, leaf age and growing way unanimity, bottom Lao Ye is cut in every strain, stays 4 leaves in top, and 35 strains are that control group, 35 strains are inoculation group.0.02mol/L phosphate buffer with 10 times of volumes (V/W), pH=7.0, grind the fresh sick leaf that infects TMV, PVY and CMV3 kind virus, get supernatant after the filtered through gauze, to tobacco several the 4th leaves inoculations from top to bottom, control group is smeared the 0.02mol/L phosphate buffer, pH=7.0 with the frictional inoculation method of routine, labelled, carry out record.Two groups of plant branches are opened in the hot-house culture of plant illumination culturing rack, light intensity 10000 luxs, 26 ± 2 ℃ of temperature, humidity 70 ± 10%.
The morning 9:00, tobacco plant is put in balance 120min in the measurement environment, from inoculating preceding 1 day to inoculating back 7 day continuous acquisition thermal imaging every day figure.Infrared thermography is fixed on the tripod photographs, and camera lens during storage, is write down the number storing of every blade apart from tobacco plant 35-55cm, and is last, copies photo to computing machine.
3. data are handled, from image smoothing, image sharpening, three aspects of histogram equalization the tobacco infrared chart is carried out the figure image intensifying respectively, adopt threshold segmentation method to extract young leaves thermal imagery zone, calculate the temperature value of each pixel in susceptible group and the not susceptible group of young leaves zone and the difference of its mean value, for disease plant, the absolute value of difference is 0.6-1.1 ℃, and for disease plant not, the absolute value of difference is 0.5-0.8 ℃.
Embodiment 4
1. choose tobacco plant 80 strains of plant height, leaf age and growing way unanimity, bottom Lao Ye is cut in every strain, stays 4 leaves in top, and 40 strains are that control group, 40 strains are inoculation group.0.02mol/L phosphate buffer with 10 times of volumes (V/W), pH=7.0, grind the fresh sick leaf that infects TMV, PVY and CMV3 kind virus, get supernatant after the filtered through gauze, to tobacco several the 4th leaves inoculations from top to bottom, control group is smeared the 0.02mol/L phosphate buffer, pH=7.0 with the frictional inoculation method of routine, labelled, carry out record.Two groups of plant branches are opened in the hot-house culture of plant illumination culturing rack, light intensity 10000 luxs, 24 ± 2 ℃ of temperature, humidity 65 ± 10%.
Afternoon 14:00, tobacco plant is put in balance 60min in the measurement environment, from inoculating preceding 1 day to inoculating back 7 day continuous acquisition thermal imaging every day figure.Infrared thermography is fixed on the tripod photographs, and camera lens during storage, is write down the number storing of every blade apart from tobacco plant 35-55cm, and is last, copies photo to computing machine.
3. data are handled, from image smoothing, image sharpening, three aspects of histogram equalization the tobacco infrared chart is carried out the figure image intensifying respectively, adopt threshold segmentation method to extract young leaves thermal imagery zone, calculate the temperature value of each pixel in susceptible group and the not susceptible group of young leaves zone and the difference of its mean value, for disease plant, the absolute value of difference is 0.5-1.3 ℃, for disease plant not, and the absolute value 0.5-0.8 of difference ℃.
Embodiment 5
1. choose tobacco plant 90 strains of plant height, leaf age and growing way unanimity, bottom Lao Ye is cut in every strain, stays 4 leaves in top, and 45 strains are that control group, 45 strains are inoculation group.0.02mol/L phosphate buffer with 10 times of volumes (V/W), pH=7.0, grind the fresh sick leaf that infects TMV, PVY and CMV3 kind virus, get supernatant after the filtered through gauze, to tobacco several the 4th leaves inoculations from top to bottom, control group is smeared the 0.02mol/L phosphate buffer, pH=7.0 with the frictional inoculation method of routine, labelled, carry out record.Two groups of plant mixing are placed on the hot-house culture of plant illumination culturing rack, but guarantee that every strain plant does not contact, infect light intensity 10000 luxs, 26 ± 2 ℃ of temperature, humidity 70 ± 10% in case cause friction.
The morning 9:00, tobacco plant is put in balance 60min in the measurement environment, from inoculating preceding 1 day to inoculating back 7 day continuous acquisition thermal imaging every day figure.Infrared thermography is fixed on the tripod photographs, and camera lens during storage, is write down the number storing of every blade apart from tobacco plant 35-55cm, and is last, copies photo to computing machine.
3. data are handled, from image smoothing, image sharpening, three aspects of histogram equalization the tobacco infrared chart is carried out the figure image intensifying respectively, adopt threshold segmentation method to extract young leaves thermal imagery zone, calculate the temperature value of susceptible group and not susceptible group each pixel of young leaves zone and the difference of its mean value, when the absolute value of difference greater than 1 ℃, be judged to disease plant, otherwise, be judged to not disease plant, the result was inoculation the 4th day, plant shows before the visible symptom, and 45 strains inoculation plant is identified entirely, namely identifies diseased plant rate and reaches 100%.
Embodiment 6
1. choose tobacco plant 100 strains of plant height, leaf age and growing way unanimity, bottom Lao Ye is cut in every strain, stays 4 leaves in top, and 50 strains are that control group, 50 strains are inoculation group.0.02mol/L phosphate buffer with 10 times of volumes (V/W), pH=7.0, grind the fresh sick leaf that infects TMV, PVY and CMV3 kind virus, get supernatant after the filtered through gauze, to tobacco several the 4th leaves inoculations from top to bottom, control group is smeared the 0.02mol/L phosphate buffer, pH=7.0 with the frictional inoculation method of routine, labelled, carry out record.Two groups of plant are mixed to be put under the booth natural conditions cultivate, temperature is 22 ± 2 ℃, humidity, 60 ± 10%.
The morning 9:00, from inoculating preceding 1 day to inoculating back 7 day continuous acquisition thermal imaging every day figure.Infrared thermography is fixed on the tripod photographs, and camera lens during storage, is write down the number storing of every blade apart from tobacco plant 35-55cm, and is last, copies photo to computing machine.
3. data are handled, from image smoothing, image sharpening, three aspects of histogram equalization the tobacco infrared chart is carried out the figure image intensifying respectively, adopt threshold segmentation method to extract young leaves thermal imagery zone, calculate the temperature value of susceptible group and not susceptible group each pixel of young leaves zone and the difference of its mean value, when the absolute value of difference greater than 1 ℃, be judged to disease plant, otherwise, be judged to not disease plant, the result was inoculation the 4th day, plant shows before the visible symptom, and 46 strains inoculation plant is identified, namely identifies diseased plant rate and reaches 92.0%.
More than just in conjunction with several specific embodiments; the present invention is further understood in exemplary illustration and help; but the embodiment detail only is for the present invention is described; do not represent the present invention and conceive whole technology embodiment down; therefore should not be construed as the total technology embodiment of the present invention is limited; some are In the view of the technician; not departing from the unsubstantiality of inventive concept changes; the for example simple change of technical characterictic or the replacement to have same or similar technique effect; as change Virus Name, change solution concentration, change temperature gap etc., all belong to protection domain of the present invention.
Claims (4)
1. tobacco virus early detection method based on infrared thermal imaging technique is characterized in that may further comprise the steps:
Step 1, set-up procedure: choose the tobacco plant n strain of plant height, leaf age and growing way unanimity, n is even number, and n 〉=50 are divided into two groups of control group and inoculation group, and control group is smeared the 0.02M phosphate buffer, inoculation group is smeared tobacco susceptible virus, and is labelled, carries out record;
Step 2, measuring process: utilize the infrared thermography that is fixed on the tripod to take, camera lens during storage, is write down the number storing of every blade apart from tobacco plant 35-55cm, copies photo to computing machine again;
Step 3, data handling procedure: computing machine carries out Treatment Analysis to the tobacco thermal imagery, the identification disease plant.
2. a kind of tobacco virus early detection method based on infrared thermal imaging technique as claimed in claim 1 is characterized in that the described thermal imagery processing procedure that the tobacco thermal imagery is carried out Treatment Analysis is specially:
Thermal imagery is carried out the figure image intensifying from image smoothing, image sharpening, three aspects of histogram equalization to the tobacco infrared chart respectively, adopt threshold segmentation method to extract young leaves and Lao Ye thermal imagery zone;
The tobacco growing environment temperature is higher than 16 ℃ and be lower than when equaling 28 ℃, and temperature jump appears in disease plant young leaves thermal imagery zone, and disease plant young leaves thermal imagery regional temperature changes evenly;
The tobacco growing environment temperature is higher than 28 ℃ and when being lower than 34 ℃, disease plant young leaves thermal imagery regional temperature changes evenly, and consistent with Lao Ye thermal imagery regional temperature, is high-temperature area, and disease plant young leaves thermal imagery zone is not low-temperature region, and Lao Ye thermal imagery zone is high-temperature area.
3. a kind of tobacco virus early detection method based on infrared thermal imaging technique as claimed in claim 1 is characterized in that the method for discrimination of described identification disease plant is as follows:
When the tobacco growing environment temperature is higher than 16 ℃ and be lower than when equaling 28 ℃, calculate the temperature value of each pixel in the young leaves zone and the difference of its mean value, when the absolute value of difference greater than 1 ℃, then be disease plant, otherwise, be disease plant not;
When the tobacco growing environment is higher than 28 ℃ and when being lower than 34 ℃, calculate in the young leaves zone and the medial temperature difference of pixel in the Lao Ye zone, when difference is lower than 0.8 ℃, then be disease plant, otherwise, then be disease plant not.
4. a kind of tobacco virus early detection method based on infrared thermal imaging technique as claimed in claim 1, it is characterized in that: described tobacco susceptible virus can be the mixed liquor of tobacco mosaic virus (TMV) TMV, marmor upsilon PVY and cucumber mosaic virus CMV.
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| CN106872044A (en) * | 2017-02-07 | 2017-06-20 | 贵阳学院 | Method based on infrared image monitoring and warning crops early disease and infected zone |
| CN106872045A (en) * | 2017-02-07 | 2017-06-20 | 贵阳学院 | With infrared image temperature difference monitoring and evaluation crops early disease and its method for degree |
| CN107314816A (en) * | 2017-06-08 | 2017-11-03 | 贵阳学院 | The multi-level information monitoring method for early warning of crops early disease |
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| CN107644200A (en) * | 2017-08-30 | 2018-01-30 | 中国农业大学 | Pest and disease damage gradient of infection detection method and system based on Android system |
| CN108169138A (en) * | 2017-12-18 | 2018-06-15 | 扬州大学 | A kind of rice lodging monitoring method using thermal infrared images |
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| CN109001244A (en) * | 2018-09-07 | 2018-12-14 | 华中农业大学 | Device and its detection method based on infrared thermal imaging technique detection Potato Quality |
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