CN103336010B - A kind of tobacco virus early detection method based on infrared thermal imaging technique - Google Patents
A kind of tobacco virus early detection method based on infrared thermal imaging technique Download PDFInfo
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Abstract
The invention discloses a kind of tobacco virus early detection method based on infrared thermal imaging technique, from image smoothing, image sharpening, histogram equalization three aspects, image enhaucament is carried out to tobacco infrared chart, adopt threshold segmentation method to extract young leaves and Lao Ye thermal imagery region; If tobacco growing environment temperature is higher than 16 DEG C and lower than when equaling 28 DEG C, calculating the temperature value of each pixel and the difference of its mean value in young leaves region, when the absolute value of difference is greater than 1 DEG C, is then disease plant, otherwise, be non-disease plant; When environment temperature is higher than 28 DEG C and lower than 34 DEG C, calculating the medial temperature difference with pixel in Lao Ye region in young leaves region, when difference is lower than 0.8 DEG C, is then disease plant, otherwise, be then non-disease plant.Step of the present invention is simple, and consuming time short, accuracy is high, more early can detect disease; Remote real-time monitoring field crop can also be applied to, for crop growth conditions, have and disease-free etc. provide in time information reliably.
Description
Technical field
The present invention relates to a kind of early detection method of tobacco virus, refer in particular to and utilize infrared thermal imaging technique to carry out the method for early detection to tobacco virus, belong to field of non destructive testing.
Background technology
Tobacco is a kind of important industrial crops, is the Important Economic source of country and local property tax, is subject to the great attention of relevant department.In recent years, tobacco virus was successively at countries in the world occurrence injury, and outbreak of epidemic often, cause huge loss to tobacco leaf production, directly dampen the enthusiasm of tobacco grower.In China, cause the occurring area of tobacco virus and the extent of injury obviously to strengthen, its field diseased plant rate is generally at 20%-40%, and heavy reaches 40%-80%, and some plot even have no harvest.After Tobacco Infected virus, leaf growth is suppressed, leaf is little, lopsided, and underproduction amplitude can reach 20%-80%, simultaneously, sick leaf uneven color after modulation, jealous poor, quality declines, and does not also have the medicament that a kind of result for the treatment of is ideal at present, a virosis disaster out of control is enough to destroy a Ge Yan district, so accomplish early diagnosis and detect in advance to seem particularly important to disease plant.
At present, in tobacco virus detects, relatively more conventional is the immunological methods such as Enzyme-linked Immunosorbent Assay reaction, Rapid detection test strip, the method has highly sensitive, high specificity and the advantage such as easy and simple to handle, but it has its limitation and deficiency---various tobacco virus has different physicochemical properties, morphological character and antigenic property, the preparation of antibody producing, mensuration and enzyme marker also just can not standardization, in addition, also can be subject to specificity, nonspecific interference during detection.In actual plantation, tobacco grower by virtue of experience, according to sick leaf reveal any symptoms judges disease, i.e. artificial sense assessment method, and this method subjectivity is strong, poor reliability, and often based on plant performance significantly, typical Disease symptoms, for time evening.
PI viral disease detection technique exist step lengthy and tedious, have breakage, be unsuitable for the deficiency that the aspect such as to be detected on a large scale in field.Infrared thermal imaging technique utilizes object each several part temperature contrast to cause infrared emanation different, and sightless for object heat radiation situation is converted to visual thermography, thus the technology of the information of acquisition.Because it has hypersensitivity to temperature, be a kind of noncontact, do not destroy, in real time, method for quick, the temperature variation being less than 0.1 DEG C can be detected, this technology maturation be applied to electrically, the field such as aviation and medical science, application in plant disease early detection is a new developing direction, also will become a new study hotspot.This technology is particularly suitable for the transpiration of remote monitoring plant, is expected to the growing state to large area tobacco plant, the information timely with or without the aspects such as disease and pest and output estimation provide reliable.
Summary of the invention
The object of the present invention is to provide a kind of tobacco virus early detection method based on infrared thermal imaging technique, realize noncontact, do not destroy, detect the extensive tobacco virus in field in real time, fast.
In order to solve above technical matters, the concrete technical scheme that the present invention adopts is as follows:
Based on a tobacco virus early detection method for infrared thermal imaging technique, it is characterized in that comprising the following steps:
Step one, set-up procedure: choose the tobacco plant n strain that plant height, leaf age and growing way are consistent, n is even number, and n >=50, be divided into control group and inoculation group two groups, control group smears 0.02M phosphate buffer, inoculation group smears tobacco susceptible virus, labelled, makes a record;
Step 2, measuring process: utilize the infrared thermography shooting be fixed on tripod, camera lens, apart from tobacco plant 35-55cm, during storage, is write down the number storing often opening blade, then copied photo to computing machine;
Step 3, data handling procedure: computing machine carries out Treatment Analysis to tobacco heat picture, identifies disease plant.
Described the thermal imagery processing procedure that tobacco heat picture carries out Treatment Analysis to be specially:
To tobacco infrared chart, image enhaucament is carried out from image smoothing, image sharpening, histogram equalization three aspects respectively to thermal imagery, adopts threshold segmentation method to extract young leaves and Lao Ye thermal imagery region;
Tobacco growing environment temperature is higher than 16 DEG C and lower than when equaling 28 DEG C, and temperature jump appears in disease plant young leaves thermal imagery region, and the change of non-disease plant young leaves thermal imagery regional temperature evenly;
When tobacco growing environment temperature is higher than 28 DEG C and lower than 34 DEG C, the change of disease plant young leaves thermal imagery regional temperature is even, and consistent with Lao Ye thermal imagery regional temperature, is high-temperature area, and non-disease plant young leaves thermal imagery region is low-temperature region, Lao Ye thermal imagery region is high-temperature area.
The method of discrimination of described identification disease plant is as follows:
When tobacco growing environment temperature is higher than 16 DEG C and lower than when equaling 28 DEG C, calculating the temperature value of each pixel and the difference of its mean value in young leaves region, when the absolute value of difference is greater than 1 DEG C, is then disease plant, otherwise, be non-disease plant;
When tobacco growing environment temperature is higher than 28 DEG C and lower than 34 DEG C, calculating the medial temperature difference with pixel in Lao Ye region in young leaves region, when difference is lower than 0.8 DEG C, is then disease plant, otherwise, be then non-disease plant.
Described tobacco susceptible virus can be the mixed liquor of tobacco mosaic virus (TMV) TMV, marmor upsilon PVY and cucumber mosaic virus CMV.
The present invention has beneficial effect.The present invention adopts infrared thermal imaging technique, utilizes object each several part temperature contrast to cause infrared emanation different, and sightless for object heat radiation situation is converted to visual thermography, have hypersensitivity to temperature.Compared with current widely used biological method, step is simple, consuming time short; Compare with manual method, objectivity is strong, and accuracy is high, the most important thing is, more early can detect disease; Can remote real-time monitoring field crop be carried out, for crop growth conditions, have and disease-free etc. provide timely exact information.
Embodiment
Below by specific embodiment, the present invention is described in further detail.
embodiment 1
1. choose the strain of plant height, leaf age and growing way consistent tobacco plant 60, bottom Lao Ye is cut in every strain, and leave 4, top leaf, 30 strains are control group, 30 strains are inoculation group.With the 0.02mol/L phosphate buffer of 10 times of volumes (V/W), pH7.0, the fresh sick leaf of TMV, PVY and CMV3 kind virus is infected in grinding, supernatant is got after filtered through gauze, with the frictional inoculation method of routine to tobacco several 4th leaf inoculation from top to bottom, control group smears 0.02mol/L phosphate buffer, pH7.0, labelled, make a record.Two groups of plant are divided and is opened in the hot-house culture of plant illumination culturing rack, light intensity 10000 lux, temperature 18 ± 2 DEG C, humidity 70 ± 10%.
2. the morning 9:00, tobacco plant is put in measurement environment and balances 60min, from inoculation first 1 day to inoculation latter 7 day every day continuous acquisition thermograph.Infrared thermography is fixed on tripod photographs, and camera lens, apart from tobacco plant 35-55cm, during storage, is write down the number storing often opening blade, finally, copied photo to computing machine.
3. data processing, from image smoothing, image sharpening, histogram equalization three aspects, image enhaucament is carried out to tobacco infrared chart respectively, threshold segmentation method is adopted to extract young leaves thermal imagery region, calculate the temperature value of each pixel and the difference of its mean value in susceptible group and not susceptible group of young leaves region, for disease plant, the absolute value of difference is 1.0-1.3 DEG C, and for non-disease plant, the absolute value of difference is 0.6-0.8 DEG C.
embodiment 2
1. choose the strain of plant height, leaf age and growing way consistent tobacco plant 50, bottom Lao Ye is cut in every strain, and leave 4, top leaf, 25 strains are control group, 25 strains are inoculation group.With the 0.02mol/L phosphate buffer of 10 times of volumes (V/W), pH7.0, the fresh sick leaf of TMV, PVY and CMV3 kind virus is infected in grinding, get supernatant after filtered through gauze, with the frictional inoculation method of routine to tobacco several 4th leaf inoculation from top to bottom, control group smears 0.02mol/L phosphate buffer, pH7.0,, labelled, make a record.Two groups of plant are divided and is opened in the hot-house culture of plant illumination culturing rack, light intensity 10000 lux, temperature 32 ± 2 DEG C, humidity 70 ± 10%.
2. the morning 9:00, tobacco plant is put in measurement environment and balances 60min, from inoculation first 1 day to inoculation latter 7 day every day continuous acquisition thermograph.Infrared thermography is fixed on tripod photographs, and camera lens, apart from tobacco plant 35-55cm, during storage, is write down the number storing often opening blade, finally, copied photo to computing machine.
3. data processing, from image smoothing, image sharpening, histogram equalization three aspects, image enhaucament is carried out to tobacco infrared chart respectively, threshold segmentation method is adopted to extract young leaves and Lao Ye thermal imagery region, calculate disease plant and the young leaves region of non-disease plant and the medial temperature difference of Lao Ye area pixel, for disease plant, temperature gap is 0.5-0.6 DEG C, and for non-disease plant, temperature gap is 1.2-1.3 DEG C
embodiment 3
1. choose the strain of plant height, leaf age and growing way consistent tobacco plant 70, bottom Lao Ye is cut in every strain, and leave 4, top leaf, 35 strains are control group, 35 strains are inoculation group.With the 0.02mol/L phosphate buffer of 10 times of volumes (V/W), pH=7.0, the fresh sick leaf of TMV, PVY and CMV3 kind virus is infected in grinding, supernatant is got after filtered through gauze, with the frictional inoculation method of routine to tobacco several 4th leaf inoculation from top to bottom, control group smears 0.02mol/L phosphate buffer, pH=7.0, labelled, make a record.Two groups of plant are divided and is opened in the hot-house culture of plant illumination culturing rack, light intensity 10000 lux, temperature 26 ± 2 DEG C, humidity 70 ± 10%.
2. the morning 9:00, tobacco plant is put in measurement environment and balances 120min, from inoculation first 1 day to inoculation latter 7 day every day continuous acquisition thermograph.Infrared thermography is fixed on tripod photographs, and camera lens, apart from tobacco plant 35-55cm, during storage, is write down the number storing often opening blade, finally, copied photo to computing machine.
3. data processing, from image smoothing, image sharpening, histogram equalization three aspects, image enhaucament is carried out to tobacco infrared chart respectively, threshold segmentation method is adopted to extract young leaves thermal imagery region, calculate the temperature value of each pixel and the difference of its mean value in susceptible group and not susceptible group of young leaves region, for disease plant, the absolute value of difference is 0.6-1.1 DEG C, and for non-disease plant, the absolute value of difference is 0.5-0.8 DEG C.
embodiment 4
1. choose the strain of plant height, leaf age and growing way consistent tobacco plant 80, bottom Lao Ye is cut in every strain, and leave 4, top leaf, 40 strains are control group, 40 strains are inoculation group.With the 0.02mol/L phosphate buffer of 10 times of volumes (V/W), pH=7.0, the fresh sick leaf of TMV, PVY and CMV3 kind virus is infected in grinding, supernatant is got after filtered through gauze, with the frictional inoculation method of routine to tobacco several 4th leaf inoculation from top to bottom, control group smears 0.02mol/L phosphate buffer, pH=7.0, labelled, make a record.Two groups of plant are divided and is opened in the hot-house culture of plant illumination culturing rack, light intensity 10000 lux, temperature 24 ± 2 DEG C, humidity 65 ± 10%.
2. afternoon 14:00, tobacco plant is put in measurement environment and balances 60min, from inoculation first 1 day to inoculation latter 7 day every day continuous acquisition thermograph.Infrared thermography is fixed on tripod photographs, and camera lens, apart from tobacco plant 35-55cm, during storage, is write down the number storing often opening blade, finally, copied photo to computing machine.
3. data processing, from image smoothing, image sharpening, histogram equalization three aspects, image enhaucament is carried out to tobacco infrared chart respectively, threshold segmentation method is adopted to extract young leaves thermal imagery region, calculate the temperature value of each pixel and the difference of its mean value in susceptible group and not susceptible group of young leaves region, for disease plant, the absolute value of difference is 0.5-1.3 DEG C, for non-disease plant, and the absolute value 0.5-0.8 DEG C of difference.
embodiment 5
1. choose the strain of plant height, leaf age and growing way consistent tobacco plant 90, bottom Lao Ye is cut in every strain, and leave 4, top leaf, 45 strains are control group, 45 strains are inoculation group.With the 0.02mol/L phosphate buffer of 10 times of volumes (V/W), pH=7.0, the fresh sick leaf of TMV, PVY and CMV3 kind virus is infected in grinding, supernatant is got after filtered through gauze, with the frictional inoculation method of routine to tobacco several 4th leaf inoculation from top to bottom, control group smears 0.02mol/L phosphate buffer, pH=7.0, labelled, make a record.Two groups of plant mixing are placed on the hot-house culture of plant illumination culturing rack, but ensure that every strain plant does not contact, in case cause friction to infect, light intensity 10000 lux, temperature 26 ± 2 DEG C, humidity 70 ± 10%.
2. the morning 9:00, tobacco plant is put in measurement environment and balances 60min, from inoculation first 1 day to inoculation latter 7 day every day continuous acquisition thermograph.Infrared thermography is fixed on tripod photographs, and camera lens, apart from tobacco plant 35-55cm, during storage, is write down the number storing often opening blade, finally, copied photo to computing machine.
3. data processing, from image smoothing, image sharpening, histogram equalization three aspects, image enhaucament is carried out to tobacco infrared chart respectively, threshold segmentation method is adopted to extract young leaves thermal imagery region, calculate the temperature value of susceptible group and the not susceptible group each pixel in young leaves region and the difference of its mean value, when the absolute value of difference is greater than 1 DEG C, be judged to disease plant, otherwise, be judged to non-disease plant, result was inoculation the 4th day, before plant shows Visual symptoms, 45 strain inoculation plant are entirely identified, namely identify that diseased plant rate reaches 100%.
embodiment 6
1. choose the strain of plant height, leaf age and growing way consistent tobacco plant 100, bottom Lao Ye is cut in every strain, and leave 4, top leaf, 50 strains are control group, 50 strains are inoculation group.With the 0.02mol/L phosphate buffer of 10 times of volumes (V/W), pH=7.0, the fresh sick leaf of TMV, PVY and CMV3 kind virus is infected in grinding, supernatant is got after filtered through gauze, with the frictional inoculation method of routine to tobacco several 4th leaf inoculation from top to bottom, control group smears 0.02mol/L phosphate buffer, pH=7.0, labelled, make a record.Cultivate under two groups of plant mixing are put in booth natural conditions, temperature is 22 ± 2 DEG C, humidity, 60 ± 10%.
2. the morning 9:00, from inoculation first 1 day to inoculation latter 7 day every day continuous acquisition thermograph.Infrared thermography is fixed on tripod photographs, and camera lens, apart from tobacco plant 35-55cm, during storage, is write down the number storing often opening blade, finally, copied photo to computing machine.
3. data processing, from image smoothing, image sharpening, histogram equalization three aspects, image enhaucament is carried out to tobacco infrared chart respectively, threshold segmentation method is adopted to extract young leaves thermal imagery region, calculate the temperature value of susceptible group and the not susceptible group each pixel in young leaves region and the difference of its mean value, when the absolute value of difference is greater than 1 DEG C, be judged to disease plant, otherwise, be judged to non-disease plant, result was inoculation the 4th day, before plant shows Visual symptoms, 46 strain inoculation plant are identified, namely identify that diseased plant rate reaches 92.0%.
Below just in conjunction with several specific embodiment; exemplary illustration and help understand the present invention further; but embodiment detail is only in order to the present invention is described; do not represent the present invention and conceive lower whole technical em-bodiments; therefore should not be construed as the technical em-bodiments total to the present invention to limit; some are In the view of technician; the unsubstantiality not departing from inventive concept is changed; such as simply change with the technical characteristic with same or similar technique effect or replace; as changed Virus Name, changing solution concentration, changing temperature gap etc., all belong to scope.
Claims (1)
1., based on a tobacco virus early detection method for infrared thermal imaging technique, it is characterized in that comprising the following steps:
Step one, set-up procedure: choose the tobacco plant n strain that plant height, leaf age and growing way are consistent, n is even number, and n >=50, be divided into control group and inoculation group two groups, control group smears 0.02M phosphate buffer, inoculation group smears tobacco susceptible virus, labelled, makes a record;
Step 2, measuring process: utilize the infrared thermography shooting be fixed on tripod, camera lens, apart from tobacco plant 35-55cm, during storage, is write down the number storing often opening blade, then copied photo to computing machine;
Step 3, data handling procedure: computing machine carries out Treatment Analysis to tobacco heat picture, identifies disease plant;
Described the thermal imagery processing procedure that tobacco heat picture carries out Treatment Analysis to be specially:
To tobacco infrared chart, image enhaucament is carried out from image smoothing, image sharpening, histogram equalization three aspects respectively to thermal imagery, adopts threshold segmentation method to extract young leaves and Lao Ye thermal imagery region;
Tobacco growing environment temperature is higher than 16 DEG C and lower than when equaling 28 DEG C, and temperature jump appears in disease plant young leaves thermal imagery region, and the change of non-disease plant young leaves thermal imagery regional temperature evenly;
When tobacco growing environment temperature is higher than 28 DEG C and lower than 34 DEG C, the change of disease plant young leaves thermal imagery regional temperature is even, and consistent with Lao Ye thermal imagery regional temperature, is high-temperature area, and non-disease plant young leaves thermal imagery region is low-temperature region, Lao Ye thermal imagery region is high-temperature area;
The method of discrimination of described identification disease plant is as follows:
When tobacco growing environment temperature is higher than 16 DEG C and lower than when equaling 28 DEG C, calculating the temperature value of each pixel and the difference of its mean value in young leaves region, when the absolute value of difference is greater than 1 DEG C, is then disease plant, otherwise, be non-disease plant;
When tobacco growing environment is higher than 28 DEG C and lower than 34 DEG C, calculating the medial temperature difference with pixel in Lao Ye region in young leaves region, when difference is lower than 0.8 DEG C, is then disease plant, otherwise, be then non-disease plant;
Described tobacco susceptible virus is the mixed liquor of tobacco mosaic virus (TMV) TMV, marmor upsilon PVY and cucumber mosaic virus CMV.
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| CN106872044A (en) * | 2017-02-07 | 2017-06-20 | 贵阳学院 | Method based on infrared image monitoring and warning crops early disease and infected zone |
| CN106872045A (en) * | 2017-02-07 | 2017-06-20 | 贵阳学院 | With infrared image temperature difference monitoring and evaluation crops early disease and its method for degree |
| CN107314816B (en) * | 2017-06-08 | 2018-12-28 | 贵阳学院 | The multi-level information monitoring method for early warning of crops early disease |
| CN107644200A (en) * | 2017-08-30 | 2018-01-30 | 中国农业大学 | Pest and disease damage gradient of infection detection method and system based on Android system |
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| CN108391542A (en) * | 2018-01-30 | 2018-08-14 | 浙江大学 | A kind of plant incubator system of automatic water and fertilizer management and Defect inspection |
| CN108519346A (en) * | 2018-03-22 | 2018-09-11 | 江苏大学 | The method that infrared thermal imagery is combined detection incubation period masaic of tomato near infrared spectrum |
| CN109001244B (en) * | 2018-09-07 | 2021-01-01 | 华中农业大学 | Device and method for detecting potato quality based on infrared thermal imaging technology |
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