CN103301178B - Prevention and the oral drugs for the treatment of fatigue syndrome - Google Patents
Prevention and the oral drugs for the treatment of fatigue syndrome Download PDFInfo
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Abstract
A kind of prevention and the oral drugs for the treatment of fatigue syndrome, the medicinal preparation for oral administration that it is made up of the raw material formulation method routinely of Herba Epimedii 4.5～21, Radix Ginseng 0.5～2 proportion by weight.Medicine of the present invention is through swimming with a load attached to the body animal model, the pharmacodynamics contrast experiment of pole-climbing and acute toxicity testing show, medicine of the present invention has notable prolongation to kunming mice swimming with a load attached to the body and pole-jump test, shows that this medicine has notable antifatigue effect, and acute toxicity testing shows mice nontoxic.
Description
Technical field
The invention belongs to, containing raw material or the medicinal preparation technical field of its product with not clear structure, specifically relate to
And to deriving from the material of plant.
Background technology
Fatigue syndrome is due to long-term substantial amounts of working and learning task and psychiatric pressure, and the loading of health surpasses
Cross the amount of regeneration of health, the most in the past, may result in health body injury.Its clinical manifestation is mainly reflected in body brain god
The tired performance of the systemic-functions such as warp, cardiovascular, digestion endocrine, skeletal muscle, as dizzy, have a headache, have a sleepless night, forgetful, low grade fever,
Muscle, arthralgia and multiple neuropsychic symptom, its basic feature for the most extremely tired, have a rest after can not alleviate, manage
Change inspection and there is no organic disease；Its cause of disease is still not clear.
Chinese medicine thinks that fatigue syndrome is, syndrome of deficiency of kidney-QI and syndrome of deficiency of spleen qi are lost by kidney qi element, sexual life, eating and drinking without temperance or
Excessive thinking is caused.
The mechanism of fatigue syndrome is multifactor to cause immunity, digestion, nerve, cardiovascular and cerebrovascular vessel, urinary system chronic
Migration, the synthesis result of progressive impairments, current Western medicine mainly uses symptomatic treatment such as electrolyte, micro-element, aminoacid, fat
Breast, tranquilizer, hormones.Uncertain therapeutic efficacy is cut and is easily caused drug dependence.Research in recent years shows, Chinese medicine, natural medicinal component
The treatment of multiformity effect many target position and conditioning fatigue syndrome more effectively and safety, obtain the attention of researcher with this.
Chinese medicine in 2003～2009, natural drug single and compound recipe have a lot of resisting fatigue to report, but it is excessive to there is prescription, proportioning and effectively
The defects such as component collection technique, cause curative effect uneven, and taking dose is excessive, makes patient compliance difference shortcoming.Along with society
Development and progress, people strengthen the progress by promoting this research subject to health perception.
Summary of the invention
The technical problem to be solved is to overcome the shortcoming of said medicine, it is provided that a kind of nontoxic pair evident in efficacy
The treatment and prevention fatigue syndrome oral drugs of effect.
Solve technical scheme that above-mentioned technical problem used it be the raw material of Chinese medicine with following proportion by weight routinely
The medicinal preparation for oral administration that formulation method is made:
Herba Epimedii 4.5～21 parts
Radix Ginseng 0.5～2 parts
The optimal raw material of Chinese medicine proportion by weight preparing medicine of the present invention is:
Herba Epimedii 10 parts
Radix Ginseng 1 part
By the medicinal preparation for oral administration that above-mentioned each component is made according to a conventional method be capsule described on galenic pharmacy or tablet or
Granule or oral liquid.
The preparation method of medicine capsule of the present invention is as follows:
1, Herba Epimedii, adds the water extraction 3 times of 10 times amount, each 1 hour, filters, and merging filtrate filters, and concentrating under reduced pressure is relative
Density is 1.05(30 DEG C) extractum add ethanol to 60% precipitation 24 hours, filter, supernatant 60～70 DEG C reclaim ethanol, concentrate
Relative density is 1.05(30 DEG C) extractum.
2, Radix Ginseng is pulverized as coarse powder, adds the water of 8 times amount, and 4 DEG C of storage cabinet mercerations 2 times, are to filter for 24 hours every time.Medicinal residues add
10 times of water extraction 2 times, are 1 hour every time, filter, merging filtrate, and decompression (-0.07～-0.08Mpa, 60 DEG C) is concentrated into phase
Density is 1.05(30 DEG C) extractum, adding ethanol to determining alcohol is 75%, stands 24 hours, filter, taking precipitate, with 3 times of water
Dissolve, filter, take supernatant and merge with extractum.
4, by above-mentioned steps 1～2 gained extractum, supernatant, concentrate as extractum, vacuum drying, pulverized 80 mesh sieves.
5, add dextrin, mix homogeneously by capsule conventional fabrication process, cross 14 mesh sieves, pelletize, dry, granulate, fill glue
Capsule, to obtain final product.Every weight 0.28g, every 2.75g Han raw material of Chinese medicine.
The preparation method of medicinal tablet of the present invention is as follows:
Raw material of Chinese medicine used by medicinal tablet of the present invention and quality proportioning and the Chinese medicine used by medicine capsule of the present invention
Raw material is identical, and the extraction process step of raw material of Chinese medicine walks with the extraction process of capsule preparation method thereof raw material of Chinese medicine of the present invention
The most identical, adjuvant used and other processing step are carried out by the conventional fabrication process of tablet.Every tablet weight 0.5g, every containing raw
Medicine 5.5g.
The preparation technology of medicine oral liquid of the present invention is as follows:
Raw material of Chinese medicine and weight proportion used by medicine oral liquid of the present invention are identical with medicine capsule of the present invention,
The extraction process step of raw material of Chinese medicine is identical with the extraction process step of capsule preparation method thereof raw material of Chinese medicine of the present invention, used
Adjuvant and other processing step are carried out according to the conventional fabrication process of oral liquid.Every bottle of 10mL, every bottle of 11g Han raw material of Chinese medicine.
The preparation technology of medicinal granule of the present invention is as follows:
In used by raw material of Chinese medicine used by medicinal granule of the present invention and quality proportioning and medicine capsule of the present invention
Medicine raw material is identical, the extraction process step of raw material of Chinese medicine and the extraction process of capsule preparation method thereof raw material of Chinese medicine of the present invention
Step is identical, and adjuvant used and other processing step are carried out by the conventional fabrication process of granule.Every bag of weight 5g, every bag contains
Raw material of Chinese medicine 11g.
The present invention shows through swimming with a load attached to the body animal model, the pharmacodynamics contrast experiment of pole-climbing and acute toxicity testing, this
Bright medicine has notable prolongation to kunming mice swimming with a load attached to the body and pole-jump test, shows that this medicine has notable antifatigue effect,
Acute toxicity testing shows mice nontoxic.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in more detail, but the invention is not restricted to these embodiments.
Embodiment 1
As a example by production medicine capsule product of the present invention 1000, raw material of Chinese medicine used and adjuvant and proportioning thereof are:
Herba Epimedii 2500g
Radix Ginseng 250g
Dextrin adds to 280g
Its preparation method is as follows:
1, taking Herba Epimedii 2500g, add the water extraction 3 times of 10 times amount, the time is each 1 hour, filters, merging filtrate, subtracts
Pressure concentrates and is 1.05(30 DEG C for relative density) extractum add the extractum of ethanol most 60%.
2, take Radix Ginseng 250g to pulverize as coarse powder, add the water of 8 times amount, 4 DEG C of mercerations 24 hours 2 times, filter.Medicinal residues add 10 times of water
Extracting 2 times, be 1 hour every time, filter, merging filtrate, decompression (-0.07～-0.08Mpa, 60 DEG C) is concentrated into relative density
For 1.05(30 DEG C) extractum, adding ethanol to determining alcohol is 75%, stands 24 hours, filter, taking precipitate, with 3 times of water dissolutioies,
Filter, take supernatant and merge with extractum.
3, by above-mentioned steps 1～2 gained extractum, supernatant；Stirring and evenly mixing, concentrates and is vacuum dried into extractum, pulverized 80
Mesh sieve.
4, add dextrin, mix homogeneously by capsule conventional fabrication process, cross 14 mesh sieves, pelletize, dry, granulate, load
In 1000 hard capsule cases, to obtain final product.Every weight 0.28g, every 2.75g Han raw material of Chinese medicine.
As a example by production medicinal tablet product of the present invention 1000, raw material of Chinese medicine used and adjuvant and proportioning thereof are:
Herba Epimedii 5000g
Radix Ginseng 500g
Starch adds to 500g
Its preparation technology is carried out by the preparation technology of Tablets.Every tablet weight 0.5g, the every 5.5g Han raw material of Chinese medicine.
As a example by production granule product 1000g of the present invention, raw material of Chinese medicine used and adjuvant and quality proportioning thereof are:
Herba Epimedii 2000g
Radix Ginseng 200g
Sucrose 400g
Dextrin adds to 1000g.
Its preparation technology is carried out by the preparation technology of granule of the present invention.Every bag of weight 5g, every bag of 11g Han raw material of Chinese medicine.
As a example by production oral liquid product 1000mL of the present invention, raw material of Chinese medicine used and adjuvant and quality proportioning thereof are:
Herba Epimedii 1000g
Radix Ginseng 100g
Sucrose 400g
Distilled water adds to 1000mL.
Its preparation technology is carried out by the preparation technology of oral liquid of the present invention.Every bottle of 10mL, every bottle of 11g Han raw material of Chinese medicine.
In the proportioning of the present embodiment, the mass parts of each component of raw material of Chinese medicine is:
Herba Epimedii 10 parts
Radix Ginseng 1 part
Embodiment 2
As a example by production medicine capsule product of the present invention 1000, raw material of Chinese medicine used and adjuvant and proportioning thereof are:
Herba Epimedii 2686g
Radix Ginseng 64g
Dextrin adds to 280g
Its preparation method is identical with the preparation technology of embodiment 1 capsule.Every weight 0.28g, every contains raw material of Chinese medicine
2.75g。
As a example by production medicinal tablet product of the present invention 1000, raw material of Chinese medicine used and adjuvant and proportioning thereof are:
Herba Epimedii 5372g
Radix Ginseng 128g
Starch adds to 500g
Its preparation method is carried out by the preparation technology of Tablets.Every tablet weight 0.5g, the every 5.5g Han raw material of Chinese medicine.
As a example by production granule product 1000g of the present invention, raw material of Chinese medicine used and adjuvant and quality proportioning thereof are:
Herba Epimedii 2149g
Radix Ginseng 51g
Sucrose 400g
Dextrin adds to 1000g.
Its preparation method is carried out by the preparation technology of granule of the present invention.Every bag of weight 5g, every bag of 11g Han raw material of Chinese medicine.
As a example by production oral liquid product 1000mL of the present invention, raw material of Chinese medicine used and adjuvant and quality proportioning thereof are:
Herba Epimedii 1074g
Radix Ginseng 26g
Sucrose 400g
Distilled water adds to 1000mL.
Its preparation method is carried out by the preparation technology of oral liquid of the present invention.Every bottle of 10mL, every bottle of 11g Han raw material of Chinese medicine.
In the proportioning of the present embodiment, the mass parts of each component of raw material of Chinese medicine is:
Herba Epimedii 21 parts
Radix Ginseng 0.5 part
Embodiment 3
As a example by production medicine capsule product of the present invention 1000, raw material of Chinese medicine used and adjuvant and proportioning thereof are:
Herba Epimedii 1904g
Radix Ginseng 846g
Dextrin adds to 280g
Its preparation method is identical with the preparation technology of embodiment 1 capsule.Every weight 0.28g, every contains raw material of Chinese medicine
2.75g。
As a example by production medicinal tablet product of the present invention 1000, raw material of Chinese medicine used and adjuvant and proportioning thereof are:
Herba Epimedii 3808g
Radix Ginseng 1692g
Starch adds to 400g
Its preparation method is carried out by the preparation technology of Tablets.Every tablet weight 0.4g, the every 5.5g Han raw material of Chinese medicine.
As a example by production granule product 1000g of the present invention, raw material of Chinese medicine used and adjuvant and quality proportioning thereof are:
Herba Epimedii 1523g
Radix Ginseng 677g
Sucrose 400g
Dextrin adds to 1000g.
Its preparation method is carried out by the preparation technology of granule of the present invention.Every bag of weight 5g, every bag of 11g Han raw material of Chinese medicine.
As a example by production oral liquid product 1000mL of the present invention, raw material of Chinese medicine used and adjuvant and quality proportioning thereof are:
Herba Epimedii 762g
Radix Ginseng 338g
Sucrose 400g
Distilled water adds to 1000ml
Its preparation method is carried out by the preparation technology of oral liquid of the present invention.Every bottle of 10mL, every bottle of 11g Han raw material of Chinese medicine.
In the proportioning of the present embodiment, the mass parts of each component of raw material of Chinese medicine is:
Herba Epimedii 4.5 parts
Radix Ginseng 2 parts
Embodiment 4
As a example by production medicine capsule product of the present invention 1000, raw material of Chinese medicine used and adjuvant and proportioning thereof are:
Herba Epimedii 2475g
Radix Ginseng 275g
Dextrin adds to 280g
Its preparation method is identical with the preparation technology of embodiment 1 capsule.Every weight 0.28g, every contains raw material of Chinese medicine
2.75g。
As a example by production medicinal tablet product of the present invention 1000, raw material of Chinese medicine used and adjuvant and proportioning thereof are:
Herba Epimedii 4950g
Radix Ginseng 550g
Starch adds to 500g
Its preparation method is carried out by the preparation technology of Tablets.Every tablet weight 0.5g, the every 5.5g Han raw material of Chinese medicine.
As a example by production granule product 1000g of the present invention, raw material of Chinese medicine used and adjuvant and quality proportioning thereof are:
Herba Epimedii 1980g
Radix Ginseng 220g
Sucrose 400g
Dextrin adds to 1000g.
Its preparation method is carried out by the preparation technology of granule of the present invention.Every bag of weight 5g, every bag of 11g Han raw material of Chinese medicine.
As a example by production oral liquid product 1000mL of the present invention, raw material of Chinese medicine used and adjuvant and quality proportioning thereof are:
Herba Epimedii 990g
Radix Ginseng 110g
Sucrose 400g
Distilled water adds to 1000mL.
Its preparation method is carried out by the preparation technology of oral liquid of the present invention.Every bottle of 10mL, every bottle of 11g Han raw material of Chinese medicine.
In the proportioning of the present embodiment, the mass parts of each component of raw material of Chinese medicine is:
Herba Epimedii 4.5 parts
Radix Ginseng 0.5 part
Embodiment 5
As a example by production medicine capsule product of the present invention 1000, raw material of Chinese medicine used and adjuvant and proportioning thereof are:
Herba Epimedii 2511g
Radix Ginseng 239g
Dextrin adds to 280g
Its preparation method is identical with the preparation technology of embodiment 1 capsule.Every weight 0.28g, every contains raw material of Chinese medicine
2.75g。
As a example by production medicinal tablet product of the present invention 1000, raw material of Chinese medicine used and adjuvant and proportioning thereof are:
Herba Epimedii 5022g
Radix Ginseng 478g
Starch adds to 500g
Its preparation method is carried out by the preparation technology of Tablets.Every tablet weight 0.5g, the every 5.5g Han raw material of Chinese medicine.
As a example by production granule product 1000g of the present invention, raw material of Chinese medicine used and adjuvant and quality proportioning thereof are:
Herba Epimedii 2009g
Radix Ginseng 191g
Sucrose 400g
Dextrin adds to 1000g.
Its preparation method is carried out by the preparation technology of granule of the present invention.Every bag of weight 5g, every bag of 11g Han raw material of Chinese medicine.
As a example by production oral liquid product 1000mL of the present invention, raw material of Chinese medicine used and adjuvant and quality proportioning thereof are:
Herba Epimedii 1004g
Radix Ginseng 96g
Sucrose 400g
Distilled water adds to 1000mL.
Its preparation method is carried out by the preparation technology of oral liquid of the present invention.Every bottle of 10mL, every bottle of 11g Han raw material of Chinese medicine.
In the proportioning of the present embodiment, the mass parts of each component of raw material of Chinese medicine is:
Herba Epimedii 21 parts
Radix Ginseng 2 parts
In order to verify medicine of the present invention to kunming mice resisting fatigue effect, applicant uses the embodiment of the present invention 1 proportioning system
The powder of standby medicine of the present invention has carried out pharmacodynamics test, and various test situation are as follows:
Test apparatus: micropipettor, model is QUYEQI, Qingyun Aeronautical Instruments & Meters Co., Beijing produce.
Test medicine powder of the present invention, is carried by Pharmacy department of No.1 Hospital Affiliated to No.4 Military Medical Univ. of PLA
Confession, lot number is XJ120601；Positive control drug is aspartic acid magnesium sheet, batch number: T17308A, the production date:
2011/07, valid until: 2016/06 company of manufacturer: Huangary girui Gyogyszergyar；It is that electrolyte replenisher can promote ammonia
With the metabolism of carbon dioxide, supplement the material generating glycogen, there is anti-fatigue effect；It is administered front pure water to prepare.
Experimental animal: two monthly age male mouse of kunming, body weight 18～22g, The Fourth Military Medical University's experimental animal center carry
Confession, the quality certification number is SCXK(army) 2002-005.Rearing conditions: divide cage, 10, every cage at random.Raise in, 24 ± 2 ° of C of room temperature,
Humidity 40-60%, automatically controls round the clock.The full nutrition pellet provided with The Fourth Military Medical University's experimental animal center is fed,
Free drinking water.Breeding observing 1 week before on-test, training on trial swimming every day 1 time, each 30 minutes, it was healthy suitable to select for totally 5 times
Mice is used for testing.Training on trial pole-climbing every day 1 time, each 10 minutes, healthy suitable mouse are selected for 5 times to be used for testing totally.
Dosage and packet: experimental animal is divided into saline control group, positive controls, test group: large, medium and small metering group.
By test product medication: gavage, capacity: mice 10ml/kg body weight.
Positive drug: by the clinic amount of being given daily (based on 70kg) 1 times, conversion mice dosage is: 0.36g/kg.
The dosage of drug test group mice of the present invention is: dosage group, 0.075g/ in 0.3g/kg heavy dose group, 0.15g/kg
Kg small dose group.
1, Loaned swimming test
Giving each group of mouse gavaging relative medicine with micropipettor, once-a-day, gavage continuously 2 days, three days mices fill the mat woven of fine bamboo strips
After taking 1 hour, carry out Loaned swimming test, the sheet lead of mouse tail heavy burden 6%, put into diameter 60cm, depth of water 40cm, water temperature 28
In the cylinder that scholar is 1 DEG C, frequently catching up with mice with thin rod, record stopwatch meter record mice swimming time, mice sinks to emerging 15
Second and swimming time terminated the record time more than 30 minutes, surpass 30 minutes and count 30 minutes, number of seconds is converted to minute divided by 60.Pull out
Drying eye moisture content and take eyeball blood, execution takes paper and blots rear liver leaflet tissue 100mg.
Data process: test data is initially with difference, then mean between one-way ANOVA variance analysis population mean
Between multiple comparisons use Post-Hoc-Tests LSD inspection；All statistical procedures use SPSS statistical software to carry out statistics
Process.Result of the test is shown in Table 1, table 2.
Table 1 present invention and saline group, aspartic acid magnesium sheet are to mice burden swimming result
* represent and compare * P ＜ 0.05 with saline group；* P ＜ 0.01* represents and compares * P ＜ 0.05 with positive group；* ＜
0.01
Conclusion (of pressure testing): when large, medium and small and positive group of dosage group relatively saline group all has prolongation mice burden swimming in the present invention
Between, heavy dose of group with positive group quite, in, group have prolongation mice burden swimming time of highly significant.In showing, group's agent
Measurer is significantly increased Endurance and antifatigue effect.
2, pole-jump test
Method: give each group of mouse gavaging relative medicine with micropipettor, once-a-day, gavage continuously 2 days, the mat woven of fine bamboo strips three days is little
After Mus gavages 1 hour, carry out pole-jump test, mice is put in upper end fixing-stable, a diameter of 0.6cm of the unsettled 40cm in lower end,
On high 120cm glass column, timing after mice firmly grasps, treat that mice falls within cushion, stop timing, Jiang mice is put in glass repeatedly
On post, catching glass column only to mice is unable, be accumulated as the mice pole-climbing time, i.e. take eyeball blood, execution takes paper and blots rear liver
Leaflet tissue 100mg/ is only.
Table 2 present invention and saline group, positive drug group mice pole-climbing result
* represent and compare * P ＜ 0.05 with saline group；* P ＜ 0.01* represents and compares * P ＜ 0.05 with positive group；* ＜
0.01
Conclusion (of pressure testing): in the present invention, large, medium and small and positive group of dosage group relatively saline group all has the prolongation mice pole-climbing time,
Heavy dose of group with positive group quite, in, group's dosage group mice pole-climbing time be obviously prolonged, in showing, group's dosage have aobvious
Write and improve Endurance and antifatigue effect.
3. the swimming with a load attached to the body side of removing test:
Method: with dosage group in the present invention and middle dosage group Herba Epimedii, Radix Ginseng is by preparation method:
(1) Herba Epimedii, adds the water extraction 3 times of 10 times amount, 1,1,1 hour successively, filters, and merging filtrate filters, and reduces pressure dense
Be condensed to relative density and be 1.05(30 DEG C) extractum add ethanol to 60% precipitation 24 hours, filter, supernatant 60～70 DEG C reclaim second
Alcohol, concentrates as extractum standby.
(2) Radix Ginseng is pulverized as coarse powder, adds the water of 8 times amount, and 4 DEG C of storage cabinet mercerations 2 times, are to filter for 24 hours every time.Medicinal residues
Adding 10 times of water extraction 2 times, be 1 hour every time, filter, merging filtrate, decompression (-0.07～-0.08Mpa, 60 DEG C) is concentrated into
Relative density is 1.05(30 DEG C) extractum, adding ethanol to determining alcohol is 75%, stands 24 hours, filter, taking precipitate, with 3 times
Water dissolution, filters, and taking supernatant concentration is that extractum is standby.
(3) Herba Epimedii is more standby than making extractum by 10:1 proportioning with Ginseng Quality.
(4) test dosage: the dosage of test group mice is that all conversions are containing crude drug amount Herba Epimedii group 1.34g/kg, Radix Ginseng group
0.133g/kg, compound recipe (medicine of the present invention) group 1.47g/kg.
(5) test method is identical with Loaned swimming test.
Table 3 saline group, compound recipe group and Herba Epimedii, Radix Ginseng group are to mice burden swimming result
* represent and compare * P ＜ 0.05 with saline group；* P ＜ 0.01* represents and compares * P ＜ 0.05 with compound recipe group；* ＜
0.01
Conclusion (of pressure testing): medicine group of the present invention relatively saline group and Herba Epimedii group, Radix Ginseng group all have notable prolongation mice swimming with a load attached to the body
The swimming time, show that Herba Epimedii and Radix Ginseng are significantly increased Endurance and antifatigue effect under rational composition of prescription.
3, biochemistry Testing index:
Method: serum urea nitrogen, blood lactase acid, hepatic glycogen all build up Science and Technology Ltd.'s test kit description by Nanjing and survey
Fixed, serum urea nitrogen lot number: 20120618 specification 50T/48 samples, blood lactase acid lot number: 20120723 specification 50T/48 samples, hepatic glycogen
Lot number: 20120720 specification 50T/48 samples, detects equipment Ep0ch-267827, instrument: constant water bath box, homogenizer, trace move
Liquid device.Normal group does not joins swimming and pole-jump test, only directly takes blood regulating liver-QI leaflet tissue 100mg/.
Table 4 is respectively organized blood lactase acid, blood urea nitrogen and hepatic glycogen testing result and is compared
Compare with saline group: * P < 0.05, * * P < 0.01；Compare with positive group: * P < 0.05, * * P < 0.01
Result divides folding: after using this medicine preparation, and on dosage level little, middle, heavy burden swimming and pole-climbing endurance substantially carry
Height, and this group high dose group does not increases and endurance increase with dosage, shows that this anti-fatigue Chinese medicine preparation, under rational dosage, has
There is significant antifatigue effect.After taking the animal movement of this agent, hepatic glycogen rise level increases and urea nitrogen levels rises
Amplitude reduces, and illustrates that this Chinese medicine preparation has the generation of delay fatigue, development significantly.This preparation has that dose is little, the cycle
Short, raising resisting fatigue, the rapid feature of fatigue alleviating.
3, acute toxicity test
Test method: take Kun Ming mice 80, trial test 20, formal test 60, often organize 20 male and female half and half,
Gastric infusion.
Trial test: powder of the present invention is added distilled water on a small quantity and gropes water-soluble situation, heated and stirred is prepared as Cmax and is
0.4g/ml suspension.With reference to grace (Horn) family name's method suddenly, take mice 20, male and female half and half, be randomly divided into 4 groups, often group 5, point
Other gavage gives the powder 0.1ml/10g of the present invention of various dose, 0.2ml/10g, 0.3ml/10g, 0.4ml/10g, Continuous Observation
72 hours, have no that overt toxicity reacts.Prompting gastric infusion is difficult to measure its LD50.
Maximum medicine-feeding test: take mice 60, male and female half and half, be randomly divided into 3 groups, often group 20, gavage gives this respectively
It is 0.4g/ml suspension that product powder is prepared as Cmax, Continuous Observation 14 days.It is administered volume and presses 0.2ml/10g every time,
0.3ml/10g, 0.4ml/10g are administered, and twice totally administration in a day, Cmax 0.4g/ml, research on maximum utilized quantity is equivalent to adult and faces
799 times of bed consumption.Observation index is observed animal activity situation immediately after being administered and is included (breathing of mice, autonomic activities and row
For movable, eye inspection indication, salivation, perpendicular hair, muscular tension, feces, urine etc.) and death condition.Every day observes These parameters,
Record 1,3,5,7,14 days body weight change and death condition.
Result of the test: after powder gastric infusion of the present invention, mice, without obvious toxic reaction, breathes normal, autonomic activities and row
Having no eyeball secretions, exophthalmos phenomenon for movable without changing, feces, urine are without exception.During observation, experimental animal is taken the photograph
Food is normal, and body weight increases, and ordinary circumstance is good.Off-test, puts to death animal and carries out anatomic observation, the heart, liver, spleen, lung, kidney, testis
The internal organs such as ball, uterus have no obvious pathological change.This test records the maximum dosage-feeding of the present invention and is equivalent to clinical people (70kg)
Intend consumption 0.02g/kg 799 times.
The function of the present invention cures mainly: Yishen Jianpi, resisting fatigue, antioxidation, appetite strengthening, improves the mental status.Main use
In prevention and treatment fatigue syndrome.
The specification of medicine of the present invention: medicine capsule of the present invention every weight 0.28g, every 2.75g Han raw material of Chinese medicine；This
Every tablet weight 0.5g of bright medicinal tablet, the every 5.5g Han crude drug；Medicinal granule of the present invention every bag weight 5g, every bag contains raw material of Chinese medicine
11g；Every bottle of 10mL of medicine oral liquid of the present invention, every bottle of 11g Han raw material of Chinese medicine.
The usage of medicine of the present invention and consumption: oral medicine capsule of the present invention, 1 time 2,2 times on the 1st；The oral present invention
Medicinal tablet, 1 time 1,2 times on the 1st；Oral medicinal granule of the present invention, boiled water takes after mixing it with water, 1 time 1 bag, 1 time on the 1st；The oral present invention
Medicine oral liquid, 1 time 1 bottle, 1 time on the 1st.
Banking system: normal temperature drying preserves.
Effect duration: 2 years.
Claims (1)
1. a prevention and the oral drugs for the treatment of fatigue syndrome, it is characterised in that it is by the Chinese medicine of following proportion by weight
Raw material is made:
Herba Epimedii 4.5 parts
Radix Ginseng 0.5 part
The preparation method of above-mentioned oral drugs is as follows:
(1) Herba Epimedii adds the water extraction 3 times of 10 times amount, each 1 hour, filters, and merging filtrate filters, concentrating under reduced pressure be 30 DEG C relatively
Density be 1.05 extractum add ethanol to 60%, precipitate 24 hours, filter, supernatant 60～70 DEG C reclaim ethanol, are concentrated into 30
DEG C relative density is the extractum of 1.05；
(2) Radix Ginseng is pulverized as coarse powder, adds the water of 8 times amount, 4 DEG C of storage cabinet mercerations 2 times, is 24 hours every time, filters, medicinal residues add 10
Times water extraction 2 times, is 1 hour every time, filters, and merging filtrate, 60 DEG C ,-0.07～-0.08MPa are evaporated to 30 DEG C of phases
Being the extractum of 1.05 to density, adding ethanol to determining alcohol is 75%, stands 24 hours, filters, taking precipitate, with 3 times of water dissolutioies,
Filter, take the Herba Epimedii extractum that supernatant prepared with step (1) and merge；
(3) by above-mentioned steps (1), (2) gained extractum, supernatant, concentrate as extractum, vacuum drying, pulverized 80 mesh sieves；
(4) press galenic pharmacy capsule, tablet, granule, oral liquid conventional fabrication process, be prepared as capsule, tablet, granule
Or oral liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310229202.9A CN103301178B (en) | 2013-06-08 | 2013-06-08 | Prevention and the oral drugs for the treatment of fatigue syndrome |
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| CN106963868A (en) * | 2016-11-09 | 2017-07-21 | 江苏金氏丹科技有限公司 | A kind of strengthen immunity Chinese medicine composition |
| CN106912926A (en) * | 2017-05-11 | 2017-07-04 | 全恒太 | A kind of composition health food and its preparation technology containing pseudo-ginseng, ginseng and Shorthorned Epimedium P.E |
| CN107875216A (en) * | 2017-11-20 | 2018-04-06 | 中国农业科学院特产研究所 | It is a kind of that there is composition for alleviating physical fatigue effect and preparation method thereof |
| CN112656870B (en) * | 2021-01-20 | 2022-07-05 | 森隆药业有限公司 | Anti-fatigue traditional Chinese medicine composition and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1785223A (en) * | 2004-12-08 | 2006-06-14 | 河北以岭医药研究院有限公司 | Medicine for treating muscular dystrophy and myasthenia gravis, and its prepn. method |
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| CN1785223A (en) * | 2004-12-08 | 2006-06-14 | 河北以岭医药研究院有限公司 | Medicine for treating muscular dystrophy and myasthenia gravis, and its prepn. method |
Non-Patent Citations (3)
| Title |
|---|
| 人参皂苷与淫羊藿苷复方制剂安全性及抗氧化功能实验研究;田辉 等;《公共卫生与预防医学》;20111231;第22卷(第3期);3-7 * |
| 活力神片的药理研究;李越剑 等;《辽宁中医学院学报》;19990630;第1卷(第2期);121-122 * |
| 淫羊藿、人参耐缺氧、耐疲劳及抗氧化作用的实验研究;邹焰 等;《遵义医学院学报》;20010228;第24卷(第1期);15-16 * |
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