CN103283479B - Cordyceps militaris strain and application thereof as well as method for producing cordyceps militaris - Google Patents
Cordyceps militaris strain and application thereof as well as method for producing cordyceps militaris Download PDFInfo
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- CN103283479B CN103283479B CN201210045940.3A CN201210045940A CN103283479B CN 103283479 B CN103283479 B CN 103283479B CN 201210045940 A CN201210045940 A CN 201210045940A CN 103283479 B CN103283479 B CN 103283479B
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Abstract
The invention discloses a cordyceps militaris strain which is one strain of cordyceps militaris, and is preserved in the CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC NO: 5606. The invention provides a method for producing the cordyceps militaris. The method comprises the steps as follows: (1), the cordyceps militaris strain is cultivated in a cultivation medium until a growth stage when an aerial hypha is produced but a sporocarp primordium is not produced; and (2), and the cordyceps militaris strain is cultivated continuously until a growth stage when cordyceps militaris sporocarp is produced. The invention provides an application of the cordyceps militaris in the production of the cordyceps militaris. According to the cordyceps militaris strain and the application thereof as well as the method for producing the cordyceps militaris, the yield and the quality of the artificially produced cordyceps militaris are improved.
Description
Technical field
The present invention relates to biological technical field, particularly, relate to a kind of cordyceps militaris link bacterial strain, the purposes of this cordyceps militaris link bacterial strain and the production method of a kind of Cordyceps militaris (L.) Link..
Background technology
Cordyceps militaris (L.) Link. (Cordyceps militaris) is a kind of entomogenous fungi, is under the jurisdiction of Ascomycota, gang pyrenomycetes, and Sphaerials, Clavicipitaceae, Cordyceps, have another name called Cordyceps militaris (L.) Link., Cordyceps militaris or pupa grass, belongs to together not of the same race with Cordyceps sinensis.Cordyceps militaris (L.) Link. has higher pharmaceutical use, see the earliest < < Compendium of Materia Medica > >: " cicada fungus can cure mainly children's sky and hang frightened epilepsy; the morbid night crying of babies; palpitaition " in addition, also has record in the Compendium of Materia Medica > > of < < Xinhua: " taste is sweet; property is flat; beneficial lung, mends marrow, hemostasis and phlegm ".Modern study shows, contains the various bioactivators such as cordycepin, Cordyceps polysaccharide in Cordyceps militaris (L.) Link., anti-inflammatory, anticancer, antibacterial, strengthen the aspects such as immunity and play an important role, similar with Cordyceps sinensis, be one of desirable substitute of Cordyceps sinensis.2009, Ministry of Health of the People's Republic of China is according to the regulation of < < Food Hygiene Law of the People's Republic of China > > and < < new resource food management method > >, approval Cordyceps militaris (L.) Link. is new resource food, and this opens up wide market for Cordyceps militaris (L.) Link. and converted products thereof and lays a good foundation.Because Cordyceps militaris (L.) Link. has high nutritive value and health-care effect, Cordyceps militaris (L.) Link. and products thereof is accepted by increasing human consumer, is particularly subject to liking of the national human consumers such as China, Japan and Korea S.
Therefore, the Application and Development of Cordyceps militaris (L.) Link. has great potential market.Tissue Culture of Cordyceps militaris is to solve the not enough good approach in wild Chinese caterpillar fungus source, relevant research is a lot, yet in artificial culture process, the hereditary property of cordyceps militaris link bacterial strain is poor, thereby cause the decline on the yield and quality of Cordyceps militaris (L.) Link., be a large bottleneck of restriction industrialization development.The poor form performance of the hereditary property of cordyceps militaris link bacterial strain is generally: angle cash resemble serious, the generation weak, fruit body primordium of mycelia growing way is less, culture cycle is long, mycelia more aging, mycelium color and luster is dark, fruiting body yield is less and sporophore abnormal rate is more high.
Therefore, providing hereditary property good cordyceps militaris link bacterial strain, for the artificial culture of Cordyceps militaris (L.) Link., is problem demanding prompt solution.
Summary of the invention
The object of this invention is to provide the good cordyceps militaris link bacterial strain of a kind of hereditary property, for the artificial culture of Cordyceps militaris (L.) Link..
To achieve these goals, on the one hand, the invention provides a kind of cordyceps militaris link bacterial strain, this cordyceps militaris link bacterial strain is a bacterial strain for Cordyceps militaris (L.) Link. (Cordyceps militaris), (address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City on December 23rd, 2011, to be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, Institute of Microorganism, Academia Sinica), preserving number is CGMCC NO:5606.This bacterial strain is that the present inventor obtains after complicated screening.
On the other hand, the invention provides the production method of a kind of Cordyceps militaris (L.) Link., the method comprises: (1) is cultured to cordyceps militaris link bacterial strain as above the growth phase that produces aerial hyphae annesl but do not produce fruit body primordium in culture material; (2) continue to be cultured to the growth phase that produces Cordyccps-militaris-(L.)-link. Sporophore; Described cultivation is cultivated and is comprised former base induction period and the sporophore growth stage of successively carrying out; While there is fruit body primordium on described aerial hyphae, described former base induction period finishes; When sporophore growth and maturity, the described sporophore growth stage finishes
In addition, the present invention also provides the purposes of cordyceps militaris link bacterial strain as above in producing Cordyceps militaris (L.) Link..
By technique scheme, the present invention has improved the yield and quality of Tissue Culture of Cordyceps militaris.
Other features and advantages of the present invention partly in detail are described the embodiment subsequently.
Embodiment
Below the specific embodiment of the present invention is elaborated.Should be understood that, embodiment described herein only, for description and interpretation the present invention, is not limited to the present invention.
In the present invention, do not do in the situation of contrary explanation, as known in the art, for fear of living contaminants, the various operations of carrying out are all preferably carried out under substantially aseptic condition, various tool used and material are had sterilized, for example, under 110-130 ℃ and 0.12-0.18MPa, carry out after steam sterilizing 20-80min.
On the one hand, the invention provides a kind of cordyceps militaris link bacterial strain, this cordyceps militaris link bacterial strain is a bacterial strain for Cordyceps militaris (L.) Link. (Cordyceps militaris), on December 23rd, 2011, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC NO:5606.This bacterial strain is that the present inventor obtains after complicated screening.
This cordyceps militaris link bacterial strain provided by the invention can be used the vegetative propagating method of conventional Cordyceps militaris (L.) Link. to go down to posterity, also can carry out preservation with conventional Cordyceps militaris spawn method for preserving, can also use the method for conventional cordyceps militaris strain rejuvenation to carry out rejuvenation and enlarged culturing.Above-mentioned cordyceps militaris link bacterial strain can carry out by the production method of Cordyceps militaris (L.) Link. the production of Cordyceps militaris (L.) Link..
On the other hand, as one of using method of above-mentioned cordyceps militaris link bacterial strain, preferably, the invention provides the production method of a kind of Cordyceps militaris (L.) Link., this production method comprises: (1) is cultured to Cordyceps militaris spawn the growth phase that produces aerial hyphae annesl but do not produce fruit body primordium in culture material; (2) continue to be cultured to the growth phase that produces Cordyccps-militaris-(L.)-link. Sporophore; Described cultivation is cultivated and is comprised former base induction period and the sporophore growth stage of successively carrying out; While there is fruit body primordium on described aerial hyphae, described former base induction period finishes; When sporophore growth and maturity, the described sporophore growth stage finishes.
Wherein, described Cordyceps militaris spawn can be commercially available various Cordyceps militaris spawns, and method of the present invention does not have special requirement to the selection of Cordyceps militaris spawn.
Wherein, described Cordyceps militaris spawn can according to the ordinary method of this area, be cultivated in advance the form for solid spawn or liquid spawn before being inoculated in culture material, was preferably the form of liquid spawn.Wherein, described Cordyceps militaris spawn can be inoculated in culture material according to conventional method, and the present invention does not have special requirement.
Wherein, the life history and grown form under Cordyceps militaris (L.) Link. carded sliver part out of office and under various culture conditions have been conventionally known to one of skill in the art, and for example (press of the Central China University of Science and Technology, publishes document for 2010 for cordyceps militaris cultivation new technology, Xu Xiuhua; New cultivation method Cordyceps militaris (L.) Link., Zhang Shengyou, press of the Central China University of Science and Technology, 2010) in record content.Due in the life history of Cordyceps militaris (L.) Link., the mycelia of Cordyceps militaris (L.) Link. can be all saffron mycelia by the mycelia annesl of white (or transparent) before there is fruit body primordium, and, because the fruit body primordium of Cordyceps militaris (L.) Link. is the mycelium of the specialization of grain of rice shape, also very easily identification.Therefore can whether come by the appearance of fruit body primordium to determine that Cordyceps militaris (L.) Link. is whether in generation aerial hyphae annesl but do not produce the growth phase of fruit body primordium.Wherein, in step (1), annesl can refer to that part annesl is safran, also can refer to that substantially all annesl is safran.
In accordance with the present production process, wherein, under preferable case, in step (1), described cultivation comprises dark cultivation stage and the light stimulation cultivation stage successively carrying out; When the mycelia in culture material is had thorough grasp culture material, described dark cultivation stage finishes; When aerial hyphae annesl is safran, described light stimulation cultivation stage finishes.
Wherein, normally, when Cordyceps militaris spawn is inoculated in culture material, Cordyceps militaris spawn is sprouted, and grows mycelia, and a part stretches into (i.e. mycelia in culture material) in culture material, a part is to culture material Growth In Space in addition, form aerial hyphae (being stretched over the mycelia in the space outside culture material), the continued growth simultaneously of the mycelia in culture material and aerial hyphae, until reach the state of the saturating culture material of mycelia of Cordyceps militaris (L.) Link..The state of the saturating culture material of mycelia of Cordyceps militaris (L.) Link. is a kind of growth conditions well known in the art, and for example, the mycelia in culture material is dispersed throughout culture material substantially, and when aerial hyphae covers culture material surface substantially, the mycelia that reaches Cordyceps militaris (L.) Link. is had thorough grasp the state of culture material.The state of having thorough grasp culture material in order to reach as soon as possible mycelia in culture material, preferably carries out the cultivation of dark cultivation stage.The condition of described dark cultivation stage can be the selection in conventional Tissue Culture of Cordyceps militaris method, as long as without under optical condition, for example the condition of described dark cultivation stage comprises: temperature is 21-27 ℃, is preferably 23-25 ℃; Relative air humidity can be 50-70%, is preferably 60%.Typically, in step (1), the time of conventional dark cultivation stage is generally 8-10 days.
In accordance with the present production process, wherein, in order to make quickly aerial hyphae annesl, it is safran, under preferable case, in step (1), the condition of described light stimulation cultivation stage comprises: the light application time of every day is 15-20 hour, and intensity of illumination is 100-150 Lux, temperature between photoperiod is 18-25 ℃, is preferably 20-23 ℃; Temperature during unglazed photograph is 18-25 ℃, is preferably 20-23 ℃.Wherein, relative air humidity can be 50-70%, is preferably 60%.Typically, in step (1), the time of conventional light stimulation cultivation stage is generally 5-7 days.
In accordance with the present production process, wherein, in step (2), described cultivation is cultivated and is the cultivation cultivation that usually said Cordyceps militaris (L.) Link. goes out the grass stage, the present invention is requirement especially not, under preferable case, described cultivation is cultivated and is comprised former base induction period and the sporophore growth stage of successively carrying out; While there is fruit body primordium on described aerial hyphae, described former base induction period finishes; When sporophore growth and maturity, the described sporophore growth stage finishes.
Wherein, because the fruit body primordium of Cordyceps militaris (L.) Link. is the aerial mycelium of the specialization of grain of rice shape, whether very easily identification, therefore can come by the appearance of fruit body primordium to determine whether former base induction period finishes.
Wherein, sporophore growth maturation refers to that sporophore growth arrives Cordyceps militaris (L.) Link. nutritive value the highest state relatively.Typically, when the size of the sporophore of Cordyceps militaris (L.) Link. is substantially no longer grown up, and while there is spinule in top, can think that sporophore growth has arrived Cordyceps militaris (L.) Link. nutritive value the highest state relatively.
In accordance with the present production process, wherein, in step (2), the condition of described former base induction period can be conventional condition, the present invention does not have special requirement, in order further to shorten the time that forms cordycep militaris fruiting body entity primodium, under preferable case, in step (2), the condition of described former base induction period comprises: the light application time of every day is 15-18 hour, intensity of illumination is 100-150 Lux, culture temperature between photoperiod is 19-24 ℃, be preferably 20-22 ℃, temperature during unglazed photograph is 13-18.5 ℃, be preferably 15-18 ℃, relative air humidity is 60-70%, be preferably 70%.Typically, in step (2), the time of conventional former base induction period is generally 12-16 days.
In accordance with the present production process, wherein, in step (2), the condition in described sporophore growth stage can be conventional condition, the present invention does not have special requirement, in order further to improve the yield and quality of Cordyceps militaris (L.) Link., under preferable case, the condition in described sporophore growth stage comprises: the light application time of every day is 12-15 hour, and intensity of illumination is 100-150 Lux, and the culture temperature between photoperiod is 16-22 ℃, be preferably 18-20 ℃, temperature during unglazed photograph is 16-22 ℃, is preferably 18-20 ℃, and relative air humidity is 80-90%.Typically, in step (2), the time in conventional sporophore growth stage is generally 15-20 days.
In accordance with the present production process, wherein, described culture material can for routine can be for the various culture materials of cultivating for material of Cordyceps militaris (L.) Link., the present invention does not have special requirement, under preferable case, contains base-material and basic nutritive medium.Wherein, the weight ratio of described base-material and described basic nutritive medium is 1: 1.1 to 1: 1.3.Wherein, described basic culture solution contains: the pupa extract of 15-25g/L, 0.6-1g/L magnesium sulfate, the potassium primary phosphate of 0.8-1.2g/L and the egg white of 50-70g/L.Wherein, described base-material contains rice and analysis for soybean powder, and the weight ratio of rice and analysis for soybean powder be 3: 1 to 4: 1 wherein, described pupa extract can dissolve for conventional water the solvend that (can for boiling dissolving) goes out from silkworm chrysalis.Described egg white can be generally the egg white of raw egg.
On the other hand, the present invention also provides the purposes of cordyceps militaris link bacterial strain as above in producing Cordyceps militaris (L.) Link..
Below will to the present invention, exemplarily describe in detail by embodiment.
Preparation Example 1
This Preparation Example cultivates cordyceps militaris link bacterial strain provided by the invention for the form of liquid spawn.
200g potato is boiled to 30min in 1L boiling water, filter and obtain potato leach liquor, then in whole potato leach liquor obtained above, add the MgSO of 20g glucose, 3g peptone, 1.5g
47H
2the KH of O and 3g
2pO
4, and be settled to 1L, under 121 ℃ and 0.14Mpa, after steam sterilizing 30min, be cooled to room temperature, obtain potato liquid nutrient medium standby.
Get Cordyceps militaris spawn (be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC NO:5606, is the Cordyceps militaris (L.) Link. mycelia tissue of preserving under 4 ℃ of conditions) the about 3mm of volume
33 of bacterial classification pieces be inoculated in 150ml potato liquid nutrient medium obtained above, at per minute 120, turn, shaking culture 7 days at 25 ℃ of temperature, obtain the Cordyceps militaris bacterial classification of this Preparation Example.
Preparation Example 2
This Preparation Example cultivates a kind of cordyceps militaris link bacterial strain being commercially available for the form of liquid spawn.
Get Cordyceps militaris spawn (purchased from Beijing agricultural and forest science institute, being the Cordyceps militaris (L.) Link. mycelia tissue of preserving under 4 ℃ of conditions) the about 3mm of volume
33 of bacterial classification pieces be inoculated in the potato liquid nutrient medium that 150ml is identical with Preparation Example 1, at per minute 120, turn, shaking culture 7 days at 25 ℃ of temperature, obtain the Cordyceps militaris bacterial classification of this Preparation Example.
Preparation Example 3
This Preparation Example is prepared culture material.
20g silkworm chrysalis is filtered and obtains filtrate and be cooled to room temperature after digestion 30min in the water of 800ml, in whole filtrate obtained above, add the MgSO of 0.8g
47H
2the KH of O, 1g
2pO
4, the peptone of 6g and the Ovum Gallus domesticus album of 60g, be settled to 1L, obtain basic nutritive medium.
After being mixed by the weight ratio of 3: 1, rice and analysis for soybean powder obtain base-material.Base-material is mixed by the weight ratio of 1: 1.2 with basic nutritive medium, under 121 ℃ and 0.14Mpa, after steam sterilizing 60min, be cooled to room temperature, obtain culture material standby.
Embodiment 1
In the culturing bottle of the culture material obtaining in the Preparation Example 2 that contains 40g, the Cordyceps militaris spawn of the liquid spawn form that the Preparation Example 1 of inoculation 3ml obtains, carries out the cultivation of dark cultivation stage.
Wherein, the cultivation of dark cultivation stage is carried out in lucifuge environment, and actual conditions comprises: temperature is 23-25 ℃, and relative air humidity can be 60%.Dark cultivation stage carried out after 8 days, and the mycelia in visible culture material is dispersed throughout culture material substantially, aerial hyphae covers culture material surface substantially, had reached the state that the mycelia in culture material is had thorough grasp culture material.Now, finish the cultivation of dark cultivation stage, then carry out the cultivation of light stimulus cultivation stage.
Wherein, the condition of light stimulation cultivation stage comprises: the light application time of every day is 18 hours, and intensity of illumination is 150 Luxs, and the temperature between photoperiod is 22 ℃, and the temperature during unglazed photograph is 22 ℃, and relative air humidity is 60%.Light stimulation cultivation stage carried out after 6 days, and visible aerial hyphae substantially whole annesls is safran and has just started to form mound shape protuberance.Now, finish light stimulation cultivation stage, and directly carry out the cultivation of former base induction period.
Wherein, the condition of former base induction period comprises: the light application time of every day is 15 hours, and intensity of illumination is 100 Luxs, and the temperature between photoperiod is 22 ℃, and the temperature during unglazed photograph is 16 ℃, and relative air humidity is 70%.Former base induction period carried out after 12 days, on visible aerial hyphae, started to form fruit body primordium, now, finished the cultivation of former base induction period and cultivated, and then carried out the cultivation in sporophore growth stage and cultivated.
Wherein, the condition in sporophore growth stage comprises that the light application time of every day is 12 hours, and intensity of illumination is 150 Luxs, and the temperature between photoperiod is 20 ℃, and the temperature during unglazed photograph is 20 ℃, and relative air humidity is 80-90%.The sporophore growth stage carried out after 17 days, and the size of the sporophore of visible Cordyceps militaris (L.) Link. is substantially no longer grown up, and spinule appears in top, and now, Cordyceps militaris (L.) Link. nutritive value is relatively the highest, finishes immediately the cultivation in sporophore growth stage and cultivates, and gathers.
In the present embodiment, from the Cordyceps militaris spawn of liquid spawn form, being inoculated into culture material starts, through 43 days, the Cordyccps-militaris-(L.)-link. Sporophore (fresh weight) of average results 22g in each culturing bottle, after dry, the shape of sporophore meet " bar-shaped, long 4-7cm, diameter 0.1-0.5cm, surperficial light brown yellow to khaki, have the thin vertical wrinkle, quality pliable and tough " standard be normal-sub entity, incongruent is lopsided sporophore, and the sporophore abnormal rate that statistics obtains the present embodiment production is 15%.
Comparative example 1
In the culturing bottle of the culture material obtaining in the Preparation Example 2 that contains 40g, the Cordyceps militaris spawn of the liquid spawn form that the Preparation Example 2 of inoculation 3ml obtains, carries out the cultivation of dark cultivation stage.
Wherein, the cultivation of dark cultivation stage is carried out in lucifuge environment, and actual conditions comprises: temperature is 23-25 ℃, and relative air humidity can be 60%.Dark cultivation stage carried out after 9 days, and the mycelia in visible culture material is dispersed throughout culture material substantially, aerial hyphae covers culture material surface substantially, had reached the state that the mycelia in culture material is had thorough grasp culture material.Now, finish the cultivation of dark cultivation stage, then carry out the cultivation of light stimulus cultivation stage.
Wherein, the condition of light stimulation cultivation stage comprises: the light application time of every day is 18 hours, and intensity of illumination is 150 Luxs, and the temperature between photoperiod is 22 ℃, and the temperature during unglazed photograph is 22 ℃, and relative air humidity is 60%.Light stimulation cultivation stage carried out after 6 days, and visible aerial hyphae substantially whole annesls is safran and has just started to form mound shape protuberance.Now, finish light stimulation cultivation stage, and directly carry out the cultivation of former base induction period.
Wherein, the condition of former base induction period comprises: the light application time of every day is 15 hours, and intensity of illumination is 100 Luxs, and the temperature between photoperiod is 22 ℃, and the temperature during unglazed photograph is 16 ℃, and relative air humidity is 70%.Former base induction period carried out after 12 days, on visible aerial hyphae, started to form fruit body primordium, now, finished the cultivation of former base induction period and cultivated, and then carried out the cultivation in sporophore growth stage and cultivated.
Wherein, the condition in sporophore growth stage comprises that the light application time of every day is 12 hours, and intensity of illumination is 150 Luxs, and the temperature between photoperiod is 20 ℃, and the temperature during unglazed photograph is 20 ℃, and relative air humidity is 80-90%.The sporophore growth stage carried out after 18 days, and the size of the sporophore of visible Cordyceps militaris (L.) Link. is substantially no longer grown up, and spinule appears in top, and now, Cordyceps militaris (L.) Link. nutritive value is relatively the highest, finishes immediately the cultivation in sporophore growth stage and cultivates, and gathers.
In this comparative example, from the Cordyceps militaris spawn of liquid spawn form, being inoculated into culture material starts, through 45 days, the Cordyccps-militaris-(L.)-link. Sporophore (fresh weight) of average results 20g in each culturing bottle, after dry, according to the method identical with embodiment 1, the sporophore abnormal rate that statistics obtains the production of this comparative example is 30%.
Contrast by embodiment 1 with comparative example 1, can find that the bacterial strain of Cordyceps militaris (L.) Link. provided by the invention has improved the yield and quality of Tissue Culture of Cordyceps militaris, is the good cordyceps militaris link bacterial strain of a kind of hereditary property.
Claims (8)
1. a cordyceps militaris link bacterial strain, this cordyceps militaris link bacterial strain is a bacterial strain for Cordyceps militaris (L.) Link. (Cordyceps militaris), is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, preserving number is CGMCC NO:5606.
2. a method of producing Cordyceps militaris (L.) Link., the method comprises:
(1) cordyceps militaris link bacterial strain claimed in claim 1 is cultured in culture material to the growth phase that produces aerial hyphae annesl but do not produce fruit body primordium;
(2) continue to be cultured to the growth phase that produces Cordyccps-militaris-(L.)-link. Sporophore; Described cultivation is cultivated and is comprised former base induction period and the sporophore growth stage of successively carrying out; While there is fruit body primordium on described aerial hyphae, described former base induction period finishes; When sporophore growth and maturity, the described sporophore growth stage finishes.
3. production method according to claim 2, wherein, in step (1), described cultivation comprises dark cultivation stage and the light stimulation cultivation stage successively carrying out; When the mycelia of Cordyceps militaris (L.) Link. is had thorough grasp culture material, described dark cultivation stage finishes; When aerial hyphae annesl is safran, described light stimulation cultivation stage finishes.
4. production method according to claim 3, wherein, in step (1), the condition of described light stimulation cultivation stage comprises: the light application time of every day is 15-20 hour, intensity of illumination is 100-150 Lux, temperature between photoperiod is 18-25 ℃, and the temperature during unglazed photograph is 18-25 ℃.
5. production method according to claim 2, wherein, in step (2), the condition of described former base induction period comprises: the light application time of every day is 15-18 hour, intensity of illumination is 100-150 Lux, culture temperature between photoperiod is 18-24 ℃, and the temperature during unglazed photograph is 13-18.5 ℃, and relative air humidity is 60-70%.
6. according to the production method described in claim 2 or 4, wherein, in step (2), the condition in described sporophore growth stage comprises: the light application time of every day is 12-15 hour, intensity of illumination is 100-150 Lux, culture temperature between photoperiod is 16-22 ℃, and the temperature during unglazed photograph is 16-22 ℃, and relative air humidity is 80-90%.
7. production method according to claim 2, wherein, described culture material contains base-material and basic nutritive medium; The weight ratio of described base-material and described basic nutritive medium is that 1:1.1 is to 1:1.3; Described basic culture solution contains: the pupa extract of 15-25g/L, 0.6-1g/L magnesium sulfate and the potassium primary phosphate of 0.8-1.2g/L and the egg white of 50-70g/L; Described base-material contains rice and analysis for soybean powder, and the weight ratio of rice and analysis for soybean powder is that 3:1 is to 4:1.
8. the purposes of cordyceps militaris link bacterial strain claimed in claim 1 in producing Cordyceps militaris (L.) Link..
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| CN1094091A (en) * | 1994-02-05 | 1994-10-26 | 李春燕 | The seed selection of cordyceps militaris excellent species and high yield cultivating method |
| CN101173220A (en) * | 2007-10-16 | 2008-05-07 | 兰著碧 | Cordyceps sinensis |
| CN101177665A (en) * | 2006-11-06 | 2008-05-14 | 张刚 | Industrial method for culturing north winter worm summer herb with high-content of cordycepin |
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|---|---|---|---|---|
| CN1094091A (en) * | 1994-02-05 | 1994-10-26 | 李春燕 | The seed selection of cordyceps militaris excellent species and high yield cultivating method |
| CN101177665A (en) * | 2006-11-06 | 2008-05-14 | 张刚 | Industrial method for culturing north winter worm summer herb with high-content of cordycepin |
| CN101173220A (en) * | 2007-10-16 | 2008-05-07 | 兰著碧 | Cordyceps sinensis |
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| 孙叶等.蛹虫草规模化人工栽培技术要点.《江苏农业科学》.2008,(第6期), |
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