CN103283479A - Cordyceps militaris strain and application thereof as well as method for producing cordyceps militaris - Google Patents
Cordyceps militaris strain and application thereof as well as method for producing cordyceps militaris Download PDFInfo
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Abstract
The invention discloses a cordyceps militaris strain which is one strain of cordyceps militaris, and is preserved in the CGMCC (China General Microbiological Culture Collection Center), and the preservation number is CGMCC NO: 5606. The invention provides a method for producing the cordyceps militaris. The method comprises the steps as follows: (1), the cordyceps militaris strain is cultivated in a cultivation medium until a growth stage when an aerial hypha is produced but a sporocarp primordium is not produced; and (2), and the cordyceps militaris strain is cultivated continuously until a growth stage when cordyceps militaris sporocarp is produced. The invention provides an application of the cordyceps militaris in the production of the cordyceps militaris. According to the cordyceps militaris strain and the application thereof as well as the method for producing the cordyceps militaris, the yield and the quality of the artificially produced cordyceps militaris are improved.
Description
Technical field
The present invention relates to biological technical field, particularly, relate to a kind of cordyceps militaris link bacterial strain, the purposes of this cordyceps militaris link bacterial strain and the production method of a kind of Cordyceps militaris.
Background technology
Cordyceps militaris (Cordyceps militaris) is a kind of entomogenous fungi, is under the jurisdiction of Ascomycota, gang pyrenomycetes, and Sphaerials, Clavicipitaceae, Cordyceps have another name called northern Chinese caterpillar Fungus, northern Chinese caterpillar fungus or pupa grass, belong to together not of the same race with Cordyceps sinensis.Cordyceps militaris has higher medical value, sees Compendium of Material Medica the earliest: " cicada fungus can cure mainly the children's sky and hang frightened epilepsy, the morbid night crying of babies, palpitaition " in addition, also has record in " Xinhua's Compendium of Materia Medica ": " it is sweet to distinguish the flavor of, and property is flat, and beneficial lung is mended marrow, hemostasis and phlegm ".Modern study shows, contains multiple bioactivators such as cordycepin, Cordyceps sinensis polysaccharide in the Cordyceps militaris, anti-inflammatory, anticancer, antibiotic, strengthen aspect such as immunity and play an important role, similar with Cordyceps sinensis, be one of desirable substitute of Cordyceps sinensis.2009, Ministry of Health of the People's Republic of China was according to the regulation of " Food Hygiene Law of the People's Republic of China " and " new resource food management method ", and the approval Cordyceps militaris is new resource food, and this is that Cordyceps militaris and converted products thereof are opened up wide market and laid a good foundation.Because Cordyceps militaris has high nutritive value and health-care efficacy, Cordyceps militaris and products thereof is accepted by increasing consumer, particularly is subjected to liking of national consumers such as China, Japan and Korea S.
Therefore, the Application and Development of Cordyceps militaris has great potential market.Artificial cultivation Cordyceps militaris is to solve the not enough good approach in wild Chinese caterpillar fungus source, relevant research is a lot, yet in the artificial incubation, the hereditary capacity of cordyceps militaris link bacterial strain is relatively poor, is a big bottleneck of restriction industrialization development thereby cause the decline on the yield and quality of Cordyceps militaris.The relatively poor form of the hereditary capacity of cordyceps militaris link bacterial strain shows and is generally: the angle is cashed and is resembled seriously, the generation weak, fruit body primordium of mycelia growing way is less, cultivation cycle is long, mycelia more easily wears out, the mycelium color and luster is dark, fruiting body yield is less and the fruit body abnormal rate is more high.
Therefore, providing hereditary capacity cordyceps militaris link bacterial strain preferably, to be used for the artificial cultivation of Cordyceps militaris, is problem demanding prompt solution.
Summary of the invention
The purpose of this invention is to provide a kind of hereditary capacity cordyceps militaris link bacterial strain preferably, to be used for the artificial cultivation of Cordyceps militaris.
To achieve these goals, on the one hand, the invention provides a kind of cordyceps militaris link bacterial strain, this cordyceps militaris link bacterial strain is the bacterial strain of Cordyceps militaris (Cordyceps militaris), (address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City to be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on December 23rd, 2011, Institute of Microorganism, Academia Sinica), preserving number is CGMCC NO:5606.This bacterial strain is to obtain after the screening of the present inventor through complexity.
On the other hand, the invention provides the production method of a kind of Cordyceps militaris, this method comprises: (1) is cultured to aforesaid cordyceps militaris link bacterial strain the vegetative stage that produces aerial hyphae and annesl but do not produce fruit body primordium in composts or fertilisers of cultivating; (2) continue to be cultured to the vegetative stage that produces fruiting bodies of cordyceps militaris; Described cultivation is cultivated and is comprised original hase induction period and the sporophore growth stage of successively carrying out; When fruit body primordium occurring on the described aerial hyphae, described original hase induction period finishes; When the fruit body growth and maturity, the described sporophore growth stage finishes
In addition, the present invention also provides the purposes of aforesaid cordyceps militaris link bacterial strain in producing Cordyceps militaris.
By technique scheme, the present invention has improved the yield and quality of artificial cultivation Cordyceps militaris.
Other features and advantages of the present invention will partly be described in detail in embodiment subsequently.
Embodiment
Below the specific embodiment of the present invention is elaborated.Should be understood that embodiment described herein only is used for description and interpretation the present invention, is not limited to the present invention.
Among the present invention, do not do under the situation of opposite explanation, as known in the art, for fear of living contaminants, the various operations of carrying out are all preferably carried out under aseptic basically condition, used various tool and material are had sterilized, after for example under 110-130 ℃ and 0.12-0.18MPa, carrying out steam sterilizing 20-80min.
On the one hand, the invention provides a kind of cordyceps militaris link bacterial strain, this cordyceps militaris link bacterial strain is the bacterial strain of Cordyceps militaris (Cordyceps militaris), is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center on December 23rd, 2011, and preserving number is CGMCC NO:5606.This bacterial strain is to obtain after the screening of the present inventor through complexity.
This cordyceps militaris link bacterial strain provided by the invention can use the conventional vegetative propagating method of Cordyceps militaris to go down to posterity, also can use conventional Cordyceps militaris spawn method for preserving to come preservation, can also use the method for conventional Cordyceps militaris spawn rejuvenation to carry out rejuvenation and enlarge cultivating.Above-mentioned cordyceps militaris link bacterial strain can carry out the production of Cordyceps militaris with the production method of Cordyceps militaris.
On the other hand, as one of using method of above-mentioned cordyceps militaris link bacterial strain, preferably, the invention provides the production method of a kind of Cordyceps militaris, this production method comprises: (1) is cultured to Cordyceps militaris spawn the vegetative stage that produces aerial hyphae and annesl but do not produce fruit body primordium in composts or fertilisers of cultivating; (2) continue to be cultured to the vegetative stage that produces fruiting bodies of cordyceps militaris; Described cultivation is cultivated and is comprised original hase induction period and the sporophore growth stage of successively carrying out; When fruit body primordium occurring on the described aerial hyphae, described original hase induction period finishes; When the fruit body growth and maturity, the described sporophore growth stage finishes.
Wherein, described Cordyceps militaris spawn can be commercially available various Cordyceps militaris spawns, and method of the present invention does not have special requirement to the selection of Cordyceps militaris spawn.
Wherein, described Cordyceps militaris spawn can according to the conventional method of this area, be cultivated the form for solid spawn or liquid spawn in advance before being inoculated in composts or fertilisers of cultivating, be preferably the form of liquid spawn.Wherein, described Cordyceps militaris spawn can be inoculated in composts or fertilisers of cultivating according to the method for routine, and the present invention does not have special requirement.
Wherein, under the Cordyceps militaris carded sliver spare out of office and various artificial condition of culture under the history of life and grown form be conventionally known to one of skill in the art, for example document (the cordyceps militaris cultivation new technology, Xu Xiuhua, publishing house of the Central China University of Science and Technology, 2010 the publication; New method cultivation Cordyceps militaris, Zhang Shengyou, publishing house of the Central China University of Science and Technology, 2010) in the record content.Because in the history of life of Cordyceps militaris, the mycelia of Cordyceps militaris can be saffron mycelia by the mycelia annesl of white (or transparent) all before fruit body primordium occurring, and, because the fruit body primordium of Cordyceps militaris is the mycelium of the specialization of grain of rice shape, also very easily identification.Therefore can whether come to determine whether Cordyceps militaris is in generation aerial hyphae and annesl but does not produce the vegetative stage of fruit body primordium by the appearance of fruit body primordium.Wherein, in the step (1), annesl can refer to that the part annesl is crocus, can refer to also that basically all annesl is crocus.
In accordance with the present production process, wherein, under the preferable case, in the step (1), described cultivation comprises dark cultivation stage and the light stimulation cultivation stage that successively carries out; When the mycelia in the composts or fertilisers of cultivating was had thorough grasp composts or fertilisers of cultivating, described dark cultivation stage finished; When the aerial hyphae annesl was crocus, described light stimulation cultivation stage finished.
Wherein, normally, when being inoculated into Cordyceps militaris spawn in the composts or fertilisers of cultivating, Cordyceps militaris spawn is sprouted, and grows mycelia, and a part stretches in the composts or fertilisers of cultivating and (namely is in the mycelia in the composts or fertilisers of cultivating), a part is grown to the space beyond the composts or fertilisers of cultivating, form aerial hyphae (namely being stretched over the mycelia in the outer space of composts or fertilisers of cultivating), the continued growth simultaneously of the mycelia in the composts or fertilisers of cultivating and aerial hyphae is until the state of the saturating composts or fertilisers of cultivating of mycelia that reaches Cordyceps militaris.The state of the saturating composts or fertilisers of cultivating of mycelia of Cordyceps militaris is a kind of growth conditions well known in the art, and for example, the mycelia in composts or fertilisers of cultivating is dispersed throughout composts or fertilisers of cultivating substantially, and when aerial hyphae covered the composts or fertilisers of cultivating surface substantially, the mycelia that namely reaches Cordyceps militaris was had thorough grasp the state of composts or fertilisers of cultivating.Have thorough grasp the state of composts or fertilisers of cultivating in order to reach mycelia in the composts or fertilisers of cultivating as soon as possible, preferably carry out the cultivation of dark cultivation stage.The condition of described dark cultivation stage can be the selection in the artificial cultivation Cordyceps militaris method of routine, as long as under no optical condition, for example the condition of described dark cultivation stage comprises: temperature is 21-27 ℃, is preferably 23-25 ℃; Relative air humidity can be 50-70%, is preferably 60%.Typically, in the step (1), the time of conventional dark cultivation stage was generally 8-10 days.
In accordance with the present production process, wherein, be crocus in order to make the aerial hyphae annesl quickly, under the preferable case, in the step (1), the condition of described light stimulation cultivation stage comprises: the light application time of every day is 15-20 hour, and intensity of illumination is the 100-150 lux, temperature between illumination period is 18-25 ℃, is preferably 20-23 ℃; Temperature during the unglazed photograph is 18-25 ℃, is preferably 20-23 ℃.Wherein, relative air humidity can be 50-70%, is preferably 60%.Typically, in the step (1), the time of conventional light stimulation cultivation stage was generally 5-7 days.
In accordance with the present production process, wherein, in the step (2), described cultivation is cultivated and is the cultivation cultivation that usually said Cordyceps militaris goes out the grass stage, the present invention is requirement especially not, and under the preferable case, described cultivation is cultivated and comprised original hase induction period and the sporophore growth stage of successively carrying out; When fruit body primordium occurring on the described aerial hyphae, described original hase induction period finishes; When the fruit body growth and maturity, the described sporophore growth stage finishes.
Wherein, because the fruit body primordium of Cordyceps militaris is the aerial mycelium of the specialization of grain of rice shape, whether therefore very easily identification can come by the appearance of fruit body primordium to determine whether the original hase induction period finishes.
Wherein, the sporophore growth maturation refers to that sporophore growth arrives Cordyceps militaris nutritive value the highest state relatively.Typically, when the size of the fruit body of Cordyceps militaris is substantially no longer grown up, and the top can think that sporophore growth has arrived Cordyceps militaris nutritive value the highest state relatively when spinule occurring.
In accordance with the present production process, wherein, in the step (2), the condition of described original hase induction period can be the condition of routine, the present invention does not have special requirement, shorten the time that forms cordycep militaris fruiting body entity primodium for further, under the preferable case, in the step (2), the condition of described original hase induction period comprises: the light application time of every day is 15-18 hour, intensity of illumination is the 100-150 lux, and the cultivation temperature between illumination period is 19-24 ℃, is preferably 20-22 ℃, temperature during the unglazed photograph is 13-18.5 ℃, be preferably 15-18 ℃, relative air humidity is 60-70%, is preferably 70%.Typically, in the step (2), the time of conventional original hase induction period was generally 12-16 days.
In accordance with the present production process, wherein, in the step (2), the condition in described sporophore growth stage can be the condition of routine, the present invention does not have special requirement, in order further to improve the yield and quality of Cordyceps militaris, under the preferable case, the condition in described sporophore growth stage comprises: the light application time of every day is 12-15 hour, and intensity of illumination is the 100-150 lux, and the cultivation temperature between illumination period is 16-22 ℃, be preferably 18-20 ℃, temperature during the unglazed photograph is 16-22 ℃, is preferably 18-20 ℃, and relative air humidity is 80-90%.Typically, in the step (2), the time in conventional sporophore growth stage was generally 15-20 days.
In accordance with the present production process, wherein, described composts or fertilisers of cultivating can be expected the various composts or fertilisers of cultivating of cultivation for the generation that can be used for Cordyceps militaris of routine, and the present invention does not have special requirement, under the preferable case, contains base-material and basic nutrient solution.Wherein, the weight ratio of described base-material and described basic nutrient solution is 1: 1.1 to 1: 1.3.Wherein, described basic culture solution contains: the potassium dihydrogen phosphate of the pupa extract of 15-25g/L, 0.6-1g/L magnesium sulfate, 0.8-1.2g/L and the egg white of 50-70g/L.Wherein, described base-material contains rice and analysis for soybean powder, and the weight ratio of rice and analysis for soybean powder be 3: 1 to 4: 1 wherein, described pupa extract can dissolve the DDGS that (can for boiling dissolving) goes out for the water of routine from silkworm chrysalis.Described egg white generally can be the egg white of raw egg.
On the other hand, the present invention also provides the purposes of aforesaid cordyceps militaris link bacterial strain in producing Cordyceps militaris.
Below will exemplarily describe in detail by the present invention of embodiment.
Preparation embodiment 1
This preparation embodiment cultivates cordyceps militaris link bacterial strain provided by the invention and is the form of liquid spawn.
The 200g potato is boiled 30min in 1L boiling water, filter and obtain the potato leachate, then in the above-mentioned whole potato leachate that obtains, add the MgSO of 20g glucose, 3g peptone, 1.5g
47H
2The KH of O and 3g
2PO
4, and be settled to 1L, be cooled to room temperature behind the steam sterilizing 30min under 121 ℃ and the 0.14Mpa, it is standby to obtain the potato liquid nutrient medium.
Get Cordyceps militaris spawn (be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC NO:5606, is the Cordyceps militaris mycelia tissue of preserving under 4 ℃ of conditions) the about 3mm of volume
33 of bacterial classification pieces be inoculated in the above-mentioned potato liquid nutrient medium that obtains of 150ml, change at per minute 120,25 ℃ of following shaken cultivation of temperature 7 days, obtain the Cordyceps militaris liquid spawn of this preparation embodiment.
Preparation embodiment 2
This preparation embodiment cultivates a kind of cordyceps militaris link bacterial strain that is commercially available and is the form of liquid spawn.
Get Cordyceps militaris spawn (available from Beijing agricultural academy of sciences, being the Cordyceps militaris mycelia tissue of preserving under 4 ℃ of conditions) the about 3mm of volume
33 of bacterial classification pieces be inoculated in the 150ml potato liquid nutrient medium identical with preparation embodiment 1, change at per minute 120,25 ℃ of following shaken cultivation of temperature 7 days, obtain the Cordyceps militaris liquid spawn of this preparation embodiment.
Preparation embodiment 3
This preparation embodiment prepares composts or fertilisers of cultivating.
The 20g silkworm chrysalis filtered behind the digestion 30min in the water of 800ml obtain filtrate and be cooled to room temperature, in the above-mentioned whole filtrate that obtains, add the MgSO of 0.8g
47H
2The KH of O, 1g
2PO
4, the peptone of 6g and 60g egg clear, be settled to 1L, obtain basic nutrient solution.
Obtain base-material after rice and analysis for soybean powder mixed by 3: 1 weight ratio.Base-material is mixed by 1: 1.2 weight ratio with basic nutrient solution, be cooled to room temperature behind the steam sterilizing 60min under 121 ℃ and the 0.14Mpa, it is standby to obtain composts or fertilisers of cultivating.
Embodiment 1
In the blake bottle of the composts or fertilisers of cultivating that the preparation embodiment 2 that contains 40g obtains, the Cordyceps militaris spawn of the liquid spawn form that the preparation embodiment 1 of inoculation 3ml obtains carries out the cultivation of dark cultivation stage.
Wherein, the cultivation of dark cultivation stage is carried out in the lucifuge environment, and actual conditions comprises: temperature is 23-25 ℃, and relative air humidity can be 60%.After dark cultivation stage carried out 8 days, the mycelia in the visible composts or fertilisers of cultivating was dispersed throughout composts or fertilisers of cultivating substantially, aerial hyphae covers the composts or fertilisers of cultivating surface substantially, had namely reached the state that the mycelia in the composts or fertilisers of cultivating is had thorough grasp composts or fertilisers of cultivating.At this moment, finish the cultivation of dark cultivation stage, carry out the cultivation of light stimulus cultivation stage then.
Wherein, the condition of light stimulation cultivation stage comprises: the light application time of every day is 18 hours, and intensity of illumination is 150 luxs, and the temperature between illumination period is 22 ℃, and the temperature during the unglazed photograph is 22 ℃, and relative air humidity is 60%.After the light stimulation cultivation stage carried out 6 days, visible aerial hyphae whole annesls basically was crocus and has just begun to form mound shape protuberance.At this moment, finish the light stimulation cultivation stage, and directly carry out the cultivation of original hase induction period.
Wherein, the condition of original hase induction period comprises: the light application time of every day is 15 hours, and intensity of illumination is 100 luxs, and the temperature between illumination period is 22 ℃, and the temperature during the unglazed photograph is 16 ℃, and relative air humidity is 70%.After the original hase induction period carries out 12 days, begin to form fruit body primordium on the visible aerial hyphae, at this moment, finish the cultivation of original hase induction period and cultivate, carry out the cultivation in sporophore growth stage then and cultivate.
Wherein, the condition in sporophore growth stage comprises that the light application time of every day is 12 hours, and intensity of illumination is 150 luxs, and the temperature between illumination period is 20 ℃, and the temperature during the unglazed photograph is 20 ℃, and relative air humidity is 80-90%.After the sporophore growth stage carried out 17 days, the size of the fruit body of visible Cordyceps militaris was substantially no longer grown up, and spinule appears in the top, and at this moment, the Cordyceps militaris nutritive value is the highest relatively, finishes the cultivation in sporophore growth stage immediately and cultivates, and gathers.
In the present embodiment, be inoculated on the composts or fertilisers of cultivating from the Cordyceps militaris spawn of liquid spawn form, through 43 days, the fruiting bodies of cordyceps militaris (fresh weight) of average results 22g in each blake bottle, after the drying, the shape of fruit body meet " bar-shaped, long 4-7cm, diameter 0.1-0.5cm, surperficial light brown yellow to khaki, have the thin vertical wrinkle, quality pliable and tough " standard be the normal-sub entity, incongruent is lopsided fruit body, and the fruit body abnormal rate that statistics obtains present embodiment production is 15%.
Comparative Examples 1
In the blake bottle of the composts or fertilisers of cultivating that the preparation embodiment 2 that contains 40g obtains, the Cordyceps militaris spawn of the liquid spawn form that the preparation embodiment 2 of inoculation 3ml obtains carries out the cultivation of dark cultivation stage.
Wherein, the cultivation of dark cultivation stage is carried out in the lucifuge environment, and actual conditions comprises: temperature is 23-25 ℃, and relative air humidity can be 60%.After dark cultivation stage carried out 9 days, the mycelia in the visible composts or fertilisers of cultivating was dispersed throughout composts or fertilisers of cultivating substantially, aerial hyphae covers the composts or fertilisers of cultivating surface substantially, had namely reached the state that the mycelia in the composts or fertilisers of cultivating is had thorough grasp composts or fertilisers of cultivating.At this moment, finish the cultivation of dark cultivation stage, carry out the cultivation of light stimulus cultivation stage then.
Wherein, the condition of light stimulation cultivation stage comprises: the light application time of every day is 18 hours, and intensity of illumination is 150 luxs, and the temperature between illumination period is 22 ℃, and the temperature during the unglazed photograph is 22 ℃, and relative air humidity is 60%.After the light stimulation cultivation stage carried out 6 days, visible aerial hyphae whole annesls basically was crocus and has just begun to form mound shape protuberance.At this moment, finish the light stimulation cultivation stage, and directly carry out the cultivation of original hase induction period.
Wherein, the condition of original hase induction period comprises: the light application time of every day is 15 hours, and intensity of illumination is 100 luxs, and the temperature between illumination period is 22 ℃, and the temperature during the unglazed photograph is 16 ℃, and relative air humidity is 70%.After the original hase induction period carries out 12 days, begin to form fruit body primordium on the visible aerial hyphae, at this moment, finish the cultivation of original hase induction period and cultivate, carry out the cultivation in sporophore growth stage then and cultivate.
Wherein, the condition in sporophore growth stage comprises that the light application time of every day is 12 hours, and intensity of illumination is 150 luxs, and the temperature between illumination period is 20 ℃, and the temperature during the unglazed photograph is 20 ℃, and relative air humidity is 80-90%.After the sporophore growth stage carried out 18 days, the size of the fruit body of visible Cordyceps militaris was substantially no longer grown up, and spinule appears in the top, and at this moment, the Cordyceps militaris nutritive value is the highest relatively, finishes the cultivation in sporophore growth stage immediately and cultivates, and gathers.
In this Comparative Examples, be inoculated on the composts or fertilisers of cultivating from the Cordyceps militaris spawn of liquid spawn form, through 45 days, the fruiting bodies of cordyceps militaris (fresh weight) of average results 20g in each blake bottle, after the drying, according to the method identical with embodiment 1, it is 30% that statistics obtains the fruit body abnormal rate that this Comparative Examples produces.
By the contrast of embodiment 1 with Comparative Examples 1, can find that the bacterial strain of Cordyceps militaris provided by the invention has improved the yield and quality of artificial cultivation Cordyceps militaris, be a kind of hereditary capacity cordyceps militaris link bacterial strain preferably.
Claims (8)
1. cordyceps militaris link bacterial strain, this cordyceps militaris link bacterial strain is the bacterial strain of Cordyceps militaris (Cordyceps militaris), is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number is CGMCC NO:5606.
2. method of producing Cordyceps militaris, this method comprises:
(1) the described cordyceps militaris link bacterial strain of claim 1 is cultured to the vegetative stage that produces aerial hyphae and annesl but do not produce fruit body primordium in composts or fertilisers of cultivating;
(2) continue to be cultured to the vegetative stage that produces fruiting bodies of cordyceps militaris; Described cultivation is cultivated and is comprised original hase induction period and the sporophore growth stage of successively carrying out; When fruit body primordium occurring on the described aerial hyphae, described original hase induction period finishes; When the fruit body growth and maturity, the described sporophore growth stage finishes.
3. production method according to claim 1, wherein, in the step (1), described cultivation comprises dark cultivation stage and the light stimulation cultivation stage that successively carries out; When the mycelia of Cordyceps militaris was had thorough grasp composts or fertilisers of cultivating, described dark cultivation stage finished; When the aerial hyphae annesl was crocus, described light stimulation cultivation stage finished.
4. production method according to claim 1 and 2, wherein, in the step (1), the condition of described light stimulation cultivation stage comprises: the light application time of every day is 15-20 hour, intensity of illumination is the 100-150 lux, temperature between illumination period is 18-25 ℃, and the temperature during the unglazed photograph is 18-25 ℃.
5. production method according to claim 1, wherein, in the step (2), the condition of described original hase induction period comprises: the light application time of every day is 15-18 hour, intensity of illumination is the 100-150 lux, cultivation temperature between illumination period is 18-24 ℃, and the temperature during the unglazed photograph is 13-18.5 ℃, and relative air humidity is 60-70%.
6. according to claim 1 or 4 described production methods, wherein, in the step (2), in the step (2), the condition in described sporophore growth stage comprises: the light application time of every day is 12-15 hour, and intensity of illumination is the 100-150 lux, and the cultivation temperature between illumination period is 16-22 ℃, temperature during the unglazed photograph is 16-22 ℃, and relative air humidity is 80-90%.
7. production method according to claim 1, wherein, described composts or fertilisers of cultivating contains base-material and basic nutrient solution; The weight ratio of described base-material and described basic nutrient solution is 1: 1.1 to 1: 1.3; Described basic culture solution contains: the potassium dihydrogen phosphate of the pupa extract of 15-25g/L, 0.6-1g/L magnesium sulfate and 0.8-1.2g/L and the egg white of 50-70g/L; Described base-material contains rice and analysis for soybean powder, and the weight ratio of rice and analysis for soybean powder is 3: 1 to 4: 1.
8. the purposes of the described cordyceps militaris link bacterial strain of claim 1 in producing Cordyceps militaris.
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| CN108901587A (en) * | 2017-04-18 | 2018-11-30 | 浙江泛亚生物医药股份有限公司 | A kind of solid culture method of cicada fungus |
| CN114410476A (en) * | 2021-10-29 | 2022-04-29 | 徐州鸿宇农业科技有限公司 | Method for culturing high-yield cordycepin silkworm chrysalis cordyceps by taking dry silkworm chrysalis after silk reeling as host |
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| CN1094091A (en) * | 1994-02-05 | 1994-10-26 | 李春燕 | The seed selection of cordyceps militaris excellent species and high yield cultivating method |
| CN101173220A (en) * | 2007-10-16 | 2008-05-07 | 兰著碧 | Cordyceps sinensis |
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| CN1094091A (en) * | 1994-02-05 | 1994-10-26 | 李春燕 | The seed selection of cordyceps militaris excellent species and high yield cultivating method |
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| CN114410476A (en) * | 2021-10-29 | 2022-04-29 | 徐州鸿宇农业科技有限公司 | Method for culturing high-yield cordycepin silkworm chrysalis cordyceps by taking dry silkworm chrysalis after silk reeling as host |
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