CN103271235B - Application of oligomeric procyanidine as feed additive for prolonging shelf lives of eggs - Google Patents
Application of oligomeric procyanidine as feed additive for prolonging shelf lives of eggs Download PDFInfo
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- CN103271235B CN103271235B CN201310111134.6A CN201310111134A CN103271235B CN 103271235 B CN103271235 B CN 103271235B CN 201310111134 A CN201310111134 A CN 201310111134A CN 103271235 B CN103271235 B CN 103271235B
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Abstract
The invention relates to a new use of oligomeric procyanidine. The new use is characterized in that the oligomeric procyanidine is added to a laying fowl feed and can prolong the shelf life of fresh eggs for above 7 days after continuous feeding for above 4 weeks, the laying fowl feed can be a complete feed, a concentrated feed or an additive premix used by laying hens, laying ducks, breeding hens, breeding ducks and quails. The addition amount of the oligomeric procyanidine in the complete feed of laying fowls is 50-200mg, and the addition proportion of the oligomeric procyanidine in the concentrated feed and the additive premix is converted according to the concentration in the complete feed. The oligomeric procyanidine is a mixture of different polymers obtained through polymerizing monomers treating 5,7,3',4'-tetrahydroxyflavane-3-ol as a nuclear parent and/or 2-6 monomers, and the above polymerization mode is C-C bonding or C-O-C bonding. The oligomeric procyanidine can be directly extracted and separated from plants, can also be obtained by decomposing high procyanidine polymers extracted from the plants through a chemical, physical or microbial, enzyme method, or is directly synthesized through a chemical method.
Description
Technical field
The present invention relates to a kind of new purposes of glucosidase procyanidins, be about to glucosidase procyanidins and make an addition in feed, feed after egg fowl a period of time, can extend the shelf-life of eggs.
Technical background
China is egg production big country, and since 1985, birds, beasts and eggs output ranks first in the world always, accounts at present the more than 40% of global birds, beasts and eggs total output.China's birds, beasts and eggs overwhelming majority is with the form consumption of fresh egg, for the 0.7-1% that only accounts for eggs total output of egg processing.Xian Dandan fowl plant could arrive in consumer's hand after collecting after a series of intermediate links such as purchaser, I and II whole seller, country fair or supermarket, even neither eat immediately after arriving in consumer's hand.Egg, in these intermediate links, due to the difference of storage method and temperature, can affect to egg quality.Storage time surpasses obviously decline of Egg Quality in 20 days at normal temperatures.Have and smear the shelf-life that the methods such as preservative film extend fresh egg at present, but processing cost is too high.
OPC (proanthocyanidins, PC) be that a class is extensively present in the flavanol compound polyphenol compound in plant, by flavanol compound monomer, formed as catechin (C), epicatechin (EC), catechin and gallate (CG), L-Epicatechin gallate (ECG) etc. and polymer thereof, conventionally 2-4 aggressiveness is called to oligomer (procyanidolic oligomers, OPC), to more than pentamer be called high polymer (procyanidolic polymers, PPC).Enter over 30 years, people are studied (Fan Mingyuan and Ye Qing to the OPC composition in hundreds of plant, 2001), existing lot of documents and Patents have been reported the extractive technique of extracting OPC from the plants such as pine bark, grape pip, sea-buckthorn, Chinese sorghum, cherry, cocoa, ginkgo, peanut, hawthorn at present.Because OPC high polymer has the effect that suppresses enteron aisle digestive ferment, limited its effect in food, so some documents and patent Introduction the isolation technics of oligomer and the degradation technique of high polymer.
Glucosidase procyanidins has very strong oxidation resistance.The antioxidation activity of OPC is approximately 50 times of VE in vivo, is VC 20 times (Zhao Chaoying and Yao little Man, 2000).That glucosidase procyanidins also has is antibacterial, anti-inflammatory, protection are cardiovascular, suppress the functions (state plants and Xu Li, 1996) such as tumour.Therefore have lot of documents and Patents to report the application of glucosidase procyanidins in food, medicine, cosmetics, but application in livestock-raising is less.Feng Meng and Feng Jinghai (2008) study discovery, and oligomeric proanthocyanidins can improve the chest muscle quality of broiler chicken under hot environment.Wang Xin etc. (2005) disclose a patent of invention: a kind of oligomeric proanthocyanidins and preparation method and application (CN1654463A), a kind of preparation technology and the application in food, health products, feed addictive and veterinary drug of oligomeric proanthocyanidins have been proposed, the oligomeric proanthocyanidins that wherein adds 400mg/kg in poultry feed does not affect egg quality, but can reduce the content of cholesterol in egg, therefore propose as the claim of producing the feed addictive of low-cholesterol egg.
Summary of the invention
The new purposes that the object of this invention is to provide a kind of glucosidase procyanidins, as the application that extends the feed addictive of quality guaranteed period of eggs.Specifically, glucosidase procyanidins is added in egg fowl feed, feed continuously more than 4 weeks, can extend fresh quality guaranteed period of eggs more than 7 days.
Glucosidase procyanidins of the present invention is with 5,7,3 ', 4 '-monomer that tetrahydroxy flavan-3-alcohol is parent nucleus, and/or the mixture of the hetero-polymer being formed by 2-6 monomer polymerization, polymerization methods is that carbon-to-carbon or carbon-oxygen-carbon are connected.
Glucosidase procyanidins of the present invention can be directly extraction, isolated from plant.
Glucosidase procyanidins of the present invention can be the OPC high polymer extracting in plant, through method decomposition such as chemistry, physics or microorganism, enzymes, forms.
Glucosidase procyanidins of the present invention can obtain by chemical method is synthetic.
Egg fowl of the present invention can be laying hen, egg duck, plants chicken, plant the laying poultries such as duck, quail.
Egg fowl feed of the present invention can be laying hen, egg duck, plants chicken, plant laying poultry complete feed, concentrated feed, the additive premixes used such as duck, quail.Wherein the addition of glucosidase procyanidins in complete feed is every kilogram of interpolation 50-200mg.Adding proportion in concentrate feed and additive premix is according to this concentration conversion.
Of the present invention experiment showed, according to method provided by the invention, can extend fresh quality guaranteed period of eggs more than at least 7 days.
The specific embodiment
The experimental technique using in following embodiment if no special instructions, is conventional method.
In following embodiment, material used, reagent etc., if no special instructions, all can obtain from commercial channels.
Experiment material and method
The proanthocyanidin that oligomeric proanthocyanidins is used ethyl acetate to extract from bark of dahurian larch, extract and form.Adopt single-factor completely randomized design, select 120 of the red commodity eggs in healthy capital in 36 week age, divide 4 processed group, 10 repetitions of each processed group, each repeats 3 chickens, 4 kinds of daily rations of interpolation 0,50,100, the 200mg/kg oligomeric proanthocyanidins of feeding respectively, basal diet is identical, by the nutritional need proportioning of recommending in the red commodity egg feeding and management in < < capital handbook > >.Experimental chicken automatic water-drinking, feeding, light application time and feeding and management, anti-epidemic measure are carried out with reference to the red commodity egg feeding and management in < < capital handbook > >.
Feed and within 28 days, within continuous 7 days, collect afterwards experiment egg that laying hen produces (every chicken is collected 5 pieces), on egg, mark chicken number and date, normal temperature storage, respectively on the same day of laying eggs, store to take for 7,14,21,28 days and repeat to measure as unit egg shape index, shell thickness, egg size, yolk color and the hangh unit of each egg, wherein egg shape index is measured with slide measure, shell thickness is measured with shell thickness analyzer, and egg size, yolk color and hangh unit are measured with multi-functional Egg Quality analyzer.
The impact of table 1 oligomeric proanthocyanidins on different storage time egg egg shape indexes
In the same row of a-c, numerical value has different lowercase subscript person significant differences (P < 0.05).
From table 1, with storage time prolongation, the egg shape index of egg does not have marked change, and oligomeric proanthocyanidins does not make significant difference to the egg shape index of different storage time eggs yet.
The impact (mm) of table 2 oligomeric proanthocyanidins on different storage time egg shell thicknesses
In the same row of a-c, numerical value has different lowercase subscript person significant differences (P < 005).
From table 2, with storage time prolongation, the shell thickness of egg does not have marked change, and oligomeric proanthocyanidins does not make significant difference to the shell thickness of different storage time eggs yet.
The impact of table 3 oligomeric proanthocyanidins on different storage time egg sizes
In the same row of a-c, numerical value has different lowercase subscript person significant differences (P < 0.05).
From table 3, with storage time prolongation, the egg size of egg reduces gradually, and the reduction of egg size is scattered and disappeared and caused by the pore on eggshell mainly due to the moisture in egg; Oligomeric proanthocyanidins does not make significant difference to the egg size of different storage time eggs, shows lost without remarkable effect to the moisture in egg of oligomeric proanthocyanidins.
The impact of table 4 oligomeric proanthocyanidins on different storage time yolk colors
In the same row of a-c, numerical value has different lowercase subscript person significant differences (P < 0.05).
From table 4, with storage time prolongation, the yolk color of egg does not have marked change, and oligomeric proanthocyanidins does not make significant difference to the yolk color of different storage time eggs yet.
The impact of table 5 oligomeric proanthocyanidins on different storage time egg hangh units
In the same row of a-c, numerical value has different lowercase subscript person significant differences (P < 0.05).
From table 5, with storage time prolongation, the hangh unit of egg significantly reduces, especially at first week that preserves.Oligomeric proanthocyanidins can significantly improve different storage time egg hangh unit numerical value, the 200mg/kg oligomeric proanthocyanidins of feeding was significantly higher than control group at first week, and the egg hangh unit of the 50-200mg/kg oligomeric proanthocyanidins of feeding from second week is all significantly higher than control group.
Hangh unit is one of index of weighing in the world bird egg freshness, the leading indicator of many countries using hangh unit as evaluation egg quality, United States Department of Agriculture's egg product standard regulation, Hough unit is greater than 72 for high-quality egg (AA), 71～60 is A level, and 59～31 is B level, is C level below 30.Under this experimental condition, normal temperature is preserved after 7d, 62 left and right that the hangh unit of control group egg just reduces, and the egg hangh unit of the oligomeric proanthocyanidins of feeding still remains on more than 70; Normal temperature is preserved after 14d, and the hangh unit of control group egg is below 54 of reduction just, only can reach B grade standard, and the egg of the oligomeric proanthocyanidins of feeding hangh unit in the time of 21 days still remain on more than 60, can arrive A grade standard.The 50-200mg/kg oligomeric proanthocyanidins of feeding as can be seen here can extend fresh quality guaranteed period of eggs at least more than 7 days.
Egg hangh unit reduces mainly due to the aquation under the effect of enzyme of the dense albumen in egg, and oligomeric proanthocyanidins has the effect of extremely strong antioxidation and CKIs hydrolase, and this may be the reason that oligomeric proanthocyanidins extends fresh quality guaranteed period of eggs.
Claims (5)
1. glucosidase procyanidins, as the application that extends the feed addictive of quality guaranteed period of eggs, is characterized in that:
In laying hen, egg duck, kind chicken, kind duck, quail complete feed used, by every kilogram of complete feed, add 50-200mg glucosidase procyanidins, or in laying hen, egg duck, kind chicken, kind duck, quail concentrated feed used and additive premix, according to the concentration conversion of 50-200mg in every kilogram of complete feed, feed continuously more than 4 weeks, can extend fresh quality guaranteed period of eggs more than 7 days.
2. application according to claim 1, is characterized in that described glucosidase procyanidins is with 5,7,3 ', 4 '-monomer that tetrahydroxy flavan-3-alcohol is parent nucleus, and/or the mixture of the hetero-polymer being formed by 2-6 monomer polymerization, polymerization methods is that carbon-to-carbon or carbon-oxygen-carbon are connected.
3. application according to claim 1, is characterized in that described glucosidase procyanidins is directly extraction, isolated from plant.
4. application according to claim 1, is characterized in that described glucosidase procyanidins is the OPC high polymer extracting in plant, through method decomposition such as chemistry, physics or microorganism, enzymes, forms.
5. application according to claim 1, is characterized in that described glucosidase procyanidins obtains by chemical method is synthetic.
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| CN104171436B (en) * | 2014-09-11 | 2016-04-06 | 东北农业大学 | The anti-cold stress of a kind of Meat-type quail and simultaneously improve feed addictive and the feed of meat |
| CN104187168B (en) * | 2014-09-11 | 2016-04-06 | 东北农业大学 | A kind of compound premix preventing quail cold stress enteritis |
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| CN100413857C (en) * | 2004-02-13 | 2008-08-27 | 杭州利欣生物科技有限公司 | Procyanidins oligomer and its preparing method and use |
| WO2011087706A1 (en) * | 2010-01-13 | 2011-07-21 | Dodds, W., Jean | Animal food compositions |
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