CN103271235A - Application of oligomeric procyanidine as feed additive for prolonging shelf lives of eggs - Google Patents
Application of oligomeric procyanidine as feed additive for prolonging shelf lives of eggs Download PDFInfo
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- CN103271235A CN103271235A CN2013101111346A CN201310111134A CN103271235A CN 103271235 A CN103271235 A CN 103271235A CN 2013101111346 A CN2013101111346 A CN 2013101111346A CN 201310111134 A CN201310111134 A CN 201310111134A CN 103271235 A CN103271235 A CN 103271235A
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Abstract
The invention relates to a new use of oligomeric procyanidine. The new use is characterized in that the oligomeric procyanidine is added to a laying fowl feed and can prolong the shelf life of fresh eggs for above 7 days after continuous feeding for above 4 weeks, the laying fowl feed can be a complete feed, a concentrated feed or an additive premix used by laying hens, laying ducks, breeding hens, breeding ducks and quails. The addition amount of the oligomeric procyanidine in the complete feed of laying fowls is 50-200mg, and the addition proportion of the oligomeric procyanidine in the concentrated feed and the additive premix is converted according to the concentration in the complete feed. The oligomeric procyanidine is a mixture of different polymers obtained through polymerizing monomers treating 5,7,3',4'-tetrahydroxyflavane-3-ol as a nuclear parent and/or 2-6 monomers, and the above polymerization mode is C-C bonding or C-O-C bonding. The oligomeric procyanidine can be directly extracted and separated from plants, can also be obtained by decomposing high procyanidine polymers extracted from the plants through a chemical, physical or microbial, enzyme method, or is directly synthesized through a chemical method.
Description
Technical field
The present invention relates to a kind of new purposes of glucosidase procyanidins, be about to glucosidase procyanidins and make an addition in the feed, after the egg fowl a period of time of feeding, can prolong the shelf-life of eggs.
Technical background
China is birds, beasts and eggs big producing countries, and birds, beasts and eggs output ranks first in the world always since 1985, accounts for more than 40% of global birds, beasts and eggs total output at present.China's birds, beasts and eggs overwhelming majority is used for the 0.7-1% that only accounts for the eggs total output of egg product processing with the form consumption of bright egg.Bright egg is after egg fowl plant collects, through arriving in consumer's hand after a series of intermediate links such as purchaser, I and II whole seller, country fair or supermarket, even neither eat immediately after arriving in consumer's hand.Egg because storage method and temperature is different, can affect to egg quality in these intermediate links.Storage time surpasses obviously decline of egg product matter in 20 days at normal temperatures.Have the shelf-life that methods such as smearing preservative film prolongs bright egg at present, but processing cost is too high.
OPC (proanthocyanidins, PC) be that a class extensively is present in the flavanol compound polyphenol compound in the plant, formed by flavanol compound monomer such as catechin (C), epicatechin (EC), catechin and gallate (CG), L-Epicatechin gallate (ECG) etc. and polymer thereof, usually the 2-4 aggressiveness is called oligomer (procyanidolic oligomers, OPC), with more than the pentamer be called high polymer (procyanidolic polymers, PPC).Advance over 30 years, people have carried out studying (Fan Mingyuan and Ye Qing to the OPC composition in hundreds of plant, 2001), at present existing lot of documents with relevant patent report from plants such as pine bark, grape pip, sea-buckthorn, Chinese sorghum, cherry, cocoa, ginkgo, peanut, hawthorn, extract the extractive technique of OPC.Because the OPC high polymer has the effect that suppresses the enteron aisle digestive ferment, has limited its effect in food, so some documents and patent have been introduced the isolation technics of oligomer and the degradation technique of high polymer.
Glucosidase procyanidins has very strong oxidation resistance.The antioxidation activity of OPC is 50 times of VE approximately in vivo, is VC 20 times (Zhao Chaoying and Yao Xiaoman, 2000).That glucosidase procyanidins also has is antibacterial, anti-inflammatory, protection are cardiovascular, suppress function (state plants and Xu Li, 1996) such as tumour.Therefore have lot of documents with relevant patent report the application of glucosidase procyanidins in food, medicine, cosmetics, but the application in livestock-raising is less.Feng Meng and Feng Jinghai (2008) discover that oligomeric proanthocyanidins can improve the chest muscle quality of broiler chicken under the hot environment.Wang Xin etc. (2005) disclose a patent of invention: a kind of oligomeric proanthocyanidins and preparation method and application (CN1654463A), a kind of preparation technology and the application in food, health products, feed addictive and veterinary drug of oligomeric proanthocyanidins have been proposed, the oligomeric proanthocyanidins that wherein adds 400mg/kg in poultry feed does not influence egg quality, but can reduce content of cholesterol in the egg, therefore propose the claim as the feed addictive of producing low-cholesterol egg.
Summary of the invention
The new purposes that the purpose of this invention is to provide a kind of glucosidase procyanidins, i.e. conduct prolongs the application of the feed addictive of quality guaranteed period of eggs.Specifically, glucosidase procyanidins is added in the egg fowl feed, feed continuously more than 4 weeks, can prolong fresh quality guaranteed period of eggs more than 7 days.
Glucosidase procyanidins of the present invention is with 5,7,3 ', 4 '-the tetrahydroxy flavan-3-alcohol is the monomer of parent nucleus, and/or the mixture of the hetero-polymer that is formed by 2-6 monomer polymerization, polymerization methods is that carbon-to-carbon or carbon-oxygen-carbon link.
Glucosidase procyanidins of the present invention can be directly extraction, isolated from plant.
Glucosidase procyanidins of the present invention can be by the OPC high polymer that extracts in the plant, and decomposition forms through methods such as chemistry, physics or microorganism, enzymes.
Glucosidase procyanidins of the present invention can obtain by chemical method is synthetic.
Egg fowl of the present invention can be laying hen, egg duck, plants chicken, plant laying poultries such as duck, quail.
Egg fowl feed of the present invention can be laying hen, egg duck, plants chicken, plant used complete feed, concentrated feed, the additive premixes of laying poultry such as duck, quail.Wherein the addition of glucosidase procyanidins in complete feed is every kilogram of interpolation 50-200mg.Adding proportion in concentrate feed and additive premix is according to this concentration conversion.
Of the present invention experiment showed, according to method provided by the invention can prolong fresh quality guaranteed period of eggs more than at least 7 days.
The specific embodiment
Employed experimental technique is conventional method if no special instructions among the following embodiment.
Used material, reagent etc. if no special instructions, all can obtain from commercial channels among the following embodiment.
Experiment material and method
Oligomeric proanthocyanidins is used ethyl acetate to extract from the proanthocyanidin that bark of dahurian larch extracts and is formed.Adopt the design of single-factor completely random, select 120 of the red commodity eggs in 36 healthy capital in age in week for use, divide 4 processed group, 10 repetitions of each processed group, each repeats 3 chickens, 4 kinds of daily rations of interpolation 0,50,100, the 200mg/kg oligomeric proanthocyanidins of feeding respectively, basal diet is identical, by " the nutritional need proportioning of recommending in the red commodity egg feeding and management in the capital handbook.The experimental chicken automatic water-drinking, feeding, light application time and feeding and management, anti-epidemic measure are with reference to " capital red commodity egg feeding and management handbook is carried out.
Continuous 7 days collection experiment eggs that laying hen produces (every chicken is collected 5 pieces) after feeding 28 days, mark chicken number and date on egg, normal temperature storage, be egg shape index, shell thickness, egg size, yolk color and the Ha Shi unit that unit measures each egg to repeat in 7,14,21,28 days in the same day of laying eggs, storage respectively, wherein egg shape index is measured with slide measure, shell thickness is measured with the shell thickness analyzer, and egg size, yolk color and Ha Shi unit measure with multi-functional egg product matter analyzer.
Table 1 oligomeric proanthocyanidins is to the influence of different storage time egg egg shape indexes
Numerical value has different lowercase subscript person significant differences (P＜0.05) in the same row of a-c.
By table 1 as seen, with the storage time prolongation, the egg shape index of egg does not have marked change, and oligomeric proanthocyanidins does not make significant difference to the egg shape index of different storage time eggs yet.
Table 2 oligomeric proanthocyanidins is to the influence (mm) of different storage time egg shell thicknesses
Numerical value has different lowercase subscript person significant differences (P＜005) in the same row of a-c.
By table 2 as seen, with the storage time prolongation, the shell thickness of egg does not have marked change, and oligomeric proanthocyanidins does not make significant difference to the shell thickness of different storage time eggs yet.
Table 3 oligomeric proanthocyanidins is to the influence of different storage time egg sizes
Numerical value has different lowercase subscript person significant differences (P＜0.05) in the same row of a-c.
By table 3 as seen, with the storage time prolongation, the egg size of egg reduces gradually, and the reduction of egg size is scattered and disappeared by the pore on the eggshell mainly due to the moisture in the egg and caused; Oligomeric proanthocyanidins does not make significant difference to the egg size of different storage time eggs, shows that oligomeric proanthocyanidins significantly acts on the lost nothing of the moisture in the egg.
Table 4 oligomeric proanthocyanidins is to the influence of different storage time yolk colors
Numerical value has different lowercase subscript person significant differences (P＜0.05) in the same row of a-c.
By table 4 as seen, with the storage time prolongation, the yolk color of egg does not have marked change, and oligomeric proanthocyanidins does not make significant difference to the yolk color of different storage time eggs yet.
Table 5 oligomeric proanthocyanidins is to the influence of difference storage time egg Ha Shi unit
Numerical value has different lowercase subscript person significant differences (P＜0.05) in the same row of a-c.
By table 5 as seen, with the storage time prolongation, the Ha Shi unit of egg significantly reduces, especially first week of preservation.Oligomeric proanthocyanidins can significantly improve different storage time egg Ha Shi unit amounts, the 200mg/kg oligomeric proanthocyanidins of feeding namely is significantly higher than control group in first week, and the egg Ha Shi unit of 50-200mg/kg oligomeric proanthocyanidins all is significantly higher than control group from feeding in second week.
Ha Shi unit is one of index of weighing in the world bird egg freshness, and many countries are the leading indicator of Ha Shi unit as evaluation egg quality, United States Department of Agriculture's egg product standard code, Hough unit is high-quality egg (AA) greater than 72,71～60 is the A level, and 59～31 is the B level, is the C level below 30.Under this experimental condition, after normal temperature was preserved 7d, the Ha Shi unit of control group egg was with regard to about 62 of reduction, and the egg Ha Shi unit of the oligomeric proanthocyanidins of feeding still remains on more than 70; After normal temperature was preserved 14d, the Ha Shi unit of control group egg is below 54 of reduction just, only can reach the B grade standard, and the egg of the oligomeric proanthocyanidins of feeding Ha Shi unit in the time of 21 days remain on still more than 60, can arrive the A grade standard.This shows that the 50-200mg/kg oligomeric proanthocyanidins of feeding can prolong fresh quality guaranteed period of eggs at least more than 7 days.
Egg Ha Shi unit reduces mainly due to the aquation under the effect of enzyme of the dense albumen in the egg, and oligomeric proanthocyanidins has the effect of extremely strong antioxidation and CKIs hydrolase, and this may be the reason that oligomeric proanthocyanidins prolongs fresh quality guaranteed period of eggs.
Claims (7)
1. glucosidase procyanidins is as the application of the feed addictive that prolongs quality guaranteed period of eggs.It is characterized in that: add glucosidase procyanidins in the feed, feed continuously more than 4 weeks, can prolong fresh quality guaranteed period of eggs more than 7 days.
2. application according to claim 1 is characterized in that described feed is laying hen, egg duck, plants chicken, plants duck, the used complete feed of quail.Add the 50-200mg glucosidase procyanidins in every kilogram of complete feed.
3. application according to claim 1, it is characterized in that described feed is laying hen, egg duck, plants chicken, plants duck, the used concentrated feed of quail or additive premix, the additive capacity of glucosidase procyanidins in concentrated feed and premix is according to the dosage conversion of 50-200mg in every kilogram of complete feed.
4. according to the described application of claim 1-3, it is characterized in that described glucosidase procyanidins is with 5,7,3 ', 4 '-the tetrahydroxy flavan-3-alcohol is the monomer of parent nucleus, and/or the mixture of the hetero-polymer that is formed by 2-6 monomer polymerization, polymerization methods is that carbon-to-carbon or carbon-oxygen-carbon link.
5. according to the described application of claim 1-4, it is characterized in that described glucosidase procyanidins is directly extraction, isolated from plant.
6. according to the described application of claim 1-4, it is characterized in that described glucosidase procyanidins is by the OPC high polymer that extracts in the plant, decomposition forms through methods such as chemistry, physics or microorganism, enzymes.
7. according to the described application of claim 1-4, it is characterized in that described glucosidase procyanidins obtains by chemical method is synthetic.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104171436A (en) * | 2014-09-11 | 2014-12-03 | 东北农业大学 | Feed additive and feed for meat-purpose quail for resisting cold stress and improving meat quality |
| CN104187168A (en) * | 2014-09-11 | 2014-12-10 | 东北农业大学 | Compound premix for preventing quail cold stress enteritis |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003063611A1 (en) * | 2002-02-01 | 2003-08-07 | Brd Consultant Office | Feed for improving egg quality |
| CN1654463A (en) * | 2004-02-13 | 2005-08-17 | 杭州利欣生物科技有限公司 | Procyanidins oligomer and its preparing method and use |
| WO2011087706A1 (en) * | 2010-01-13 | 2011-07-21 | Dodds, W., Jean | Animal food compositions |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2003063611A1 (en) * | 2002-02-01 | 2003-08-07 | Brd Consultant Office | Feed for improving egg quality |
| CN1654463A (en) * | 2004-02-13 | 2005-08-17 | 杭州利欣生物科技有限公司 | Procyanidins oligomer and its preparing method and use |
| WO2011087706A1 (en) * | 2010-01-13 | 2011-07-21 | Dodds, W., Jean | Animal food compositions |
Non-Patent Citations (3)
| Title |
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| 何沙沙等: "原花青素的生物学功能及开发利用前景", 《湖南农业科学》, no. 13, 31 July 2011 (2011-07-31) * |
| 吕丽爽: "天然抗氧化剂低聚原花青素的研究进展", 《食品科学》, vol. 23, no. 2, 28 February 2002 (2002-02-28) * |
| 甄莉: "松针 、 陈皮和何首乌对鹌鹑产蛋的影响", 《饲草与饲料》, no. 4, 30 April 2011 (2011-04-30) * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104171436A (en) * | 2014-09-11 | 2014-12-03 | 东北农业大学 | Feed additive and feed for meat-purpose quail for resisting cold stress and improving meat quality |
| CN104187168A (en) * | 2014-09-11 | 2014-12-10 | 东北农业大学 | Compound premix for preventing quail cold stress enteritis |
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| CN103271235B (en) | 2014-04-23 |
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