CN103244468A - Ejecting-type jet flow cavitation generator and cavitation cell disruption device - Google Patents
Ejecting-type jet flow cavitation generator and cavitation cell disruption device Download PDFInfo
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- CN103244468A CN103244468A CN2013101334823A CN201310133482A CN103244468A CN 103244468 A CN103244468 A CN 103244468A CN 2013101334823 A CN2013101334823 A CN 2013101334823A CN 201310133482 A CN201310133482 A CN 201310133482A CN 103244468 A CN103244468 A CN 103244468A
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Abstract
The invention discloses an ejecting-type jet flow cavitation generator and a cavitation cell disruption device. The cavitation generator comprises a nozzle, a suction chamber, a throat pipe, a diffuser and an outlet pipe. The nozzle is connected in the suction chamber in a sleeved manner, and a suction pipe is arranged on the side of the suction chamber. The cavitation cell disruption device comprises a container, a high-pressure centrifugal pump and the above cavitation generator, wherein the container is used for storing cells to be disrupted, the high-pressure centrifugal pump is connected with the container through a water pipe, and the cavitation generator is connected with the high-pressure centrifugal pump through the pipe. Valves are respectively arranged on an entrance of the nozzle, the suction pipe and the outlet pipe to control degree of cavitation. In the cavitation cell disruption device, high-pressure liquid sucks up low-pressure liquid to cause cavitation to happen in the tail end of the suction chamber and the throat pipe, cells are disrupted by microjet and shock waves produced from bubble collapse, and drastic shearing actions between the other two fluids can also have tearing effect to the cells. The ejecting-type jet flow cavitation generator and the cell disruption device for cavitation cells are simple in structure, low in cost, free of moving components, reliable in operating, strong in cavitation transport, high in crushing efficiency, and thereby applicable to mass application.
Description
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Technical field
The invention belongs to the cell breaking technology field, be specifically related to relate to a kind of brand-new injection type jet flow cavitation generator and cavitation cell breaking plant.
Background technique
Method of cell disruption mainly contains at present: mechanical crushing method, chemistry and biochemistry osmose process and physics osmose process.
Mechanical crushing method comprises methods such as high pressure homogenate, pearl mill, bump fragmentation and ultrasonication again, is characterized in that treating capacity is big, crushing efficiency is high, speed is fast, mainly by homogeneous effect and the effect of milling; In the processing procedure, cell wall can't stand powerful mechanical shear stress and fragmentation, thereby causes the kytoplasm outflow.Yet heating is the subject matter that mechanical crushing method exists, and the localized hyperthermia that produces because of friction, bump can cause material required in the kytoplasm rotten.
Chemistry and biochemistry osmose process mainly comprise: methods such as soda acid is handled, chemical reagent is handled, enzyme is molten.Compare with mechanical crushing method, chemosmosis method processing rate is low, and crushing efficiency is poor, and the interpolation of chemistry or biochemical reagents can form new pollution, makes troubles for further separation and purification.
The physics osmose process mainly comprises the osmotic pressure ballistic method and freezes-the thawing method.The former is applicable to the fragmentation with the more weak cell of cell wall or cell wall intensity.The latter is applicable to the thalline of comparison fragility, yet multigelation can make protein denaturation, thereby influences the recovery rate of reactive protein.
At present, existing scholar proposes cavitation process is applied to cytoclastic concept, but is subject to the design of cavitation generator, is difficult to promote always.
Summary of the invention
The objective of the invention is to propose a kind of simple in structure, with low cost, controllability and injection type jet flow cavitation generator workable, that be suitable for promoting.
Another object of the present invention is that a kind of energy consumption of proposition is low, crushing efficiency is high, the injection type jet flow cavitation cell breaking plant based on the Hydrodynamic cavitation effect of the use that can be mass-produced.
For solving the problems of the technologies described above, the present invention adopts following technological scheme:
A kind of injection type jet flow cavitation generator, comprise nozzle, suction chamber, trunnion, diffusing tube and outer pipe, suction chamber, trunnion, diffusing tube and outer pipe are communicated with successively, socket nozzle in the suction chamber, nozzle comprises nozzle entrance section and jet expansion section, suction chamber also is provided with suction pipe, and the section area of diffusing tube increases gradually from an end a to end that links to each other with outlet section that links to each other with trunnion.
Above-mentioned diffusing tube is taper or tubaeform.
Above-mentioned suction chamber comprises cylindrical portion and platform shape section, and the end that the end area of described shape section is bigger links to each other with cylindrical portion, and the less end of its end area then links to each other with trunnion.
A kind of injection type jet flow cavitation cell breaking plant, comprise above-mentioned cavitation generator, centrifugal pump and deposit the cell container of cell solution to be broken, the two ends of centrifugal pump are communicated with nozzle entrance section and the cell internal tank of cavitation generator by pipeline respectively, and the suction pipe of cavitation generator and outer pipe are communicated with cell solution to be broken in the cell container by pipeline respectively.
Above-mentioned trunnion and diffusing tube can adopt the transparent material preparation, are used for observing cavitation condition, conveniently cavitation condition are regulated control.
The pipeline of above-mentioned connection centrifugal pump and cell container is provided with first valve, is used for shutting down maintenance.
The above-mentioned pipeline that links to each other with nozzle entrance section, suction pipe and the outer pipe of cavitation generator is respectively equipped with second valve, the 3rd valve and the 4th valve, is used for controlling the cavitation occurrence degree.
Cell breaking plant of the present invention pumps into the nozzle of cavitation generator by centrifugal pump with the cell solution in the cell container, and the pressurized working fluid at nozzle entrance place goes out interruption-forming high speed operation fluid by behind the nozzle at it, sprays into suction chamber; Under the effect of entrainmenting of high speed operation fluid, the low pressure cell solution in the cell container entrainments by suction pipe and enters suction chamber in suction chamber, and low pressure cell solution and high pressure cell solution form violent blending in suction chamber and trunnion.Trunnion makes the cavitation development obtain the cavity cloud for the terminal cavity production that produces of suction chamber provides environment under low pressure, and the blending for two fluid streams does not provide a suitable environment.Diffusing tube is used for providing pressure increased pressure environment gradually, and the cavity cloud that produces in the trunnion is transported to diffusing tube, vanishes in diffusing tube, produces microjet and shock wave, thereby realizes the fragmentation of cell cavitation.Cell after the fragmentation enters the cell container with cell solution through outer pipe.
In the preferred version of the present invention, in each gateway valve is set all, is used for fluid flow and the pressure of each inlet/outlet of control, thereby reach the control to different cavitation condition in the cavitation generator.
Cell breaking plant of the present invention utilizes highly pressurised liquid that centrifugal pump produces to entrainment low pressure cell solution in the cell container by injection type jet flow cavitation generator.Pour suction chamber and taken place by the low speed liquid that entrainmented that injection type jet flow cavitation generator can produce local depression in violent shearing motion and this process because highly pressurised liquid becomes high-velocity fluid through nozzle, can in suction chamber and trunnion, bring out cavitation phenomenon and produce a large amount of cavitys, the microjet that utilizes cavity in trunnion and diffusing tube, to crumble and fall and produce in the process, high-pressure shocking wave, pressure pulsation, hot localised points are torn cell, change permeability of cell membrane, make endochylema flow out.High-speed jet follows violent shear action that cell itself is had the effect of tearing equally in addition.In the preferred version of the present invention, be provided with some valves at each import and export, can be by each inlet/outlet parameter of valve regulated with the convenient cavitation phenomenon that obtains in various degree.
Compare with prior art, the present invention has following advantage:
(1) cavitation generator architecture of the present invention is simple, with low cost, movement-less part, and reliable operation is suitable for large-scale promotion application.
(2) the cavitation generator adopts the shearing jet to bring out cavitation, can produce a large amount of cavity clouds, and is strong to the transport property of cavity.
(3) cell breaking plant energy consumption of the present invention is low, clasmatosis efficient height, but large-scale promotion is used.
(4) clasmatosis mechanism of the present invention is mainly mechanical crushing, need not to use chemical reagent, can avoid the chemical oxidation effect to cytoplasm solution.
(5) cell breaking plant of the present invention is controlled extent of cavitation by valve, can obtain cavitation effect in various degree according to the actual requirements, has improved the controllability of device greatly.
Description of drawings
Fig. 1 is a kind of specific embodiments of cell breaking plant of the present invention;
Fig. 2 is a kind of specific embodiments of cavitation generator of the present invention.
Wherein, 1-cell container, 2-first valve, the 3-centrifugal pump,, 4-second valve, 5-the 3rd valve, 6-cavitation generator, 7-the 4th valve, 8 the 5th-valves, 61-suction pipe, 62-nozzle entrance section, 63-jet expansion section, 64-suction chamber, 65-trunnion, 66-diffusing tube, 67-outer pipe.
Embodiment
Below by embodiment also by reference to the accompanying drawings, technological scheme of the present invention is described in further detail.
The high pressure centrifugal pump 3 that this is concrete implements to comprise a cell container 1 that is used for storage cell solution to be broken, link to each other with cell container 1 by pipeline, export the cavitation generator 6 that links to each other by pipeline and high pressure centrifugal pump 3, see Fig. 1.
Comprise 5 valves in this concrete enforcement, see Fig. 1.Wherein, second valve 4, the 3rd valve 5 and the 4th valve 7 are used for controlling extent of cavitation; First valve 1 is used for shutting down maintenance; The 5th valve 8 is used for the cell solution after the fragmentation is got rid of the cell container.
The section area of diffusing tube 66 increases gradually from an end a to end that links to each other with outlet section that links to each other with trunnion, and the diffusing tube in this concrete enforcement is taper, sees Fig. 2.Because the pressurization of diffusing tube, the cavity cloud can be crumbled and fall suddenly in diffusing tube, and supervenes microjet and shock wave, thereby realizes the fragmentation to cell.
To introduce the use of concrete above-mentioned cell breaking plant below.
1, the preparatory stage
Prepare cell solution: cell solution to be broken is injected cell container 1.
Centrifugal pump starts to be prepared: open first valve 2, the 3rd valve 5 and the 4th valve 7, close second valve 4 and the 5th valve 8.
2, working stage
The start up period: see Fig. 1, start high pressure centrifugal pump 3.Open second valve 4, working fluid is pumped into the suction chamber 64 of cavitation generator 6 by high pressure centrifugal pump 3.Simultaneously, low pressure cell solution in the cell container 1 constantly is inhaled into chamber 64 inner high voltage working fluids by the 3rd valve 5 and entrainments into suction chamber 64, pressurized working fluid and low pressure cell solution carry out violent blending in suction chamber 64 and trunnion 65, and then bring out cavitation phenomenon, and in trunnion and diffusing tube, crumble and fall by cavity and realize the clasmatosis process.Solution after the cavitation process enters cell container 1 through diffusing tube 66, outer pipe 67, the 4th valve 7.
Cavitation is regulated the stage: make and occur in various degree cavitation phenomenon in suction chamber 64 and the trunnion 65 by regulating second valve 4, the 3rd valve 5 and the 4th valve 7.During adjusting, both can regulate above-mentioned three valves simultaneously, also can only regulate two valves, even only need regulate a valve.Generally speaking, above-mentioned any two valves can be kept certain aperture, regulate the another one valve to obtain cavitation phenomenon in various degree.In the concrete enforcement of basis, fix second valve 4 and the 3rd valve 5, the 4th valve 7 is regulated to obtain the extent of cavitation of needs.The specific operation step is, second valve 4 and the 3rd valve 5 are transferred to maximum opening, regulates the aperture of the 4th valve 7 then from small to large, just can obtain cavitation inception, violent cavitation and three kinds of cavitation phenomenons in various degree of limit cavitation successively.
3, quit work the stage
When needs quit work, close second valve 4, high pressure centrifugal pump 3, the 3rd valve 5 and the 4th valve 7 successively, open the 5th valve 8 then to obtain the cell solution after the processing.
Specific embodiment described herein only is that the present invention's spirit is illustrated.Those skilled in the art can make various modifications or replenish or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present invention or surmount the defined scope of appended claims.
Terms such as cell container 1, first valve 2, centrifugal pump 3, second valve 4, the 3rd valve 5, cavitation generator 6, the 4th valve 7, the 5th valve 8, suction pipe 61, nozzle entrance section 62, jet expansion section 63, suction chamber 64, trunnion 65, diffusing tube 66, outer pipe 67, but do not get rid of the possibility of using other terms.Using these terms only is in order to describe and explain essence of the present invention more easily; They are construed to any additional restriction all is contrary with spirit of the present invention.
Claims (5)
1. an injection type jet flow cavitation generator is characterized in that, comprising:
Nozzle, suction chamber, trunnion, diffusing tube and outer pipe, suction chamber, trunnion, diffusing tube and outer pipe are communicated with successively, socket nozzle in the suction chamber, nozzle comprises nozzle entrance section and jet expansion section, suction chamber is provided with suction pipe.
2. injection type jet flow cavitation generator as claimed in claim 1 is characterized in that:
Described diffusing tube is taper or tubaeform.
3. injection type jet flow cavitation cell breaking plant is characterized in that:
Comprise claim 1 or 2 described cavitation generators, centrifugal pump and deposit the cell container of cell solution to be broken, the two ends of centrifugal pump are communicated with nozzle entrance section and the cell internal tank of cavitation generator by pipeline respectively, and the suction pipe of cavitation generator and outer pipe are communicated with cell solution to be broken in the cell container by pipeline respectively.
4. injection type jet flow cavitation cell breaking plant as claimed in claim 3 is characterized in that:
The pipeline of described connection centrifugal pump and cell container is provided with first valve.
5. injection type jet flow cavitation cell breaking plant as claimed in claim 3 is characterized in that:
The described pipeline that links to each other with nozzle entrance section, suction pipe and the outer pipe of cavitation generator is respectively equipped with second valve, the 3rd valve and the 4th valve.
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| CN201310133482.3A CN103244468B (en) | 2013-04-17 | 2013-04-17 | Injection type jet flow cavitation generator and cavitation cell breaking plant |
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| CN103244468B CN103244468B (en) | 2015-11-04 |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104154049A (en) * | 2014-08-07 | 2014-11-19 | 苏州淮通电气有限公司 | Jet pump |
| CN104861038A (en) * | 2015-05-08 | 2015-08-26 | 永联生物科技(上海)有限公司 | Homogenizing valve group |
| CN105626595A (en) * | 2015-12-11 | 2016-06-01 | 中国航空工业集团公司金城南京机电液压工程研究中心 | Double-end jet pump |
| CN105668973A (en) * | 2016-04-14 | 2016-06-15 | 湖南工业大学 | Spiral-flow type jammed cavitation device for breaking excess sludge |
| CN105758754A (en) * | 2016-02-29 | 2016-07-13 | 中国矿业大学 | Test device for cavitation erosion and abrasion |
| CN106070602A (en) * | 2016-07-01 | 2016-11-09 | 武汉大学 | One realizes sterilization of milk and homogenizing integral method and device |
| CN106136018A (en) * | 2016-07-14 | 2016-11-23 | 武汉大学 | A kind of fruit juice enzyme denaturing, sterilization and homogenizing integral method and device |
| CN106172703A (en) * | 2016-07-11 | 2016-12-07 | 杨超坤 | A kind of aquatic products special bio antistaling agent process units |
| CN106630370A (en) * | 2015-11-04 | 2017-05-10 | 东莞源控环保科技有限公司 | Hydrodynamic cavitation phosphorus removal device, and sewage treatment system and method applying device |
| CN106669584A (en) * | 2016-12-21 | 2017-05-17 | 武汉大学 | Intense shearing annular jet cavitation generator |
| CN107988063A (en) * | 2017-12-28 | 2018-05-04 | 广州聚能纳米生物科技股份有限公司 | A kind of split type clasmatosis structure |
| CN109721637A (en) * | 2019-01-17 | 2019-05-07 | 梧州学院 | A kind of protein crushing plant |
| CN109967429A (en) * | 2019-03-30 | 2019-07-05 | 山东大学 | One kind being used for the clean integrated device of medical instrument heating disinfection |
| CN110496415A (en) * | 2019-07-31 | 2019-11-26 | 武汉大学 | A kind of injection type cavitation extracts method, application and its device used of anthocyanin |
| CN111974237A (en) * | 2020-08-10 | 2020-11-24 | 大连海事大学 | Cavitation generator with special-shaped flow channel |
| CN114471362A (en) * | 2022-01-29 | 2022-05-13 | 武汉大学 | Jet type super-cavitation generation system and super-cavitation generation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104154049A (en) * | 2014-08-07 | 2014-11-19 | 苏州淮通电气有限公司 | Jet pump |
| CN106286425A (en) * | 2014-08-07 | 2017-01-04 | 充梦霞 | Jet pump |
| CN104861038A (en) * | 2015-05-08 | 2015-08-26 | 永联生物科技(上海)有限公司 | Homogenizing valve group |
| CN106630370A (en) * | 2015-11-04 | 2017-05-10 | 东莞源控环保科技有限公司 | Hydrodynamic cavitation phosphorus removal device, and sewage treatment system and method applying device |
| CN106630370B (en) * | 2015-11-04 | 2019-07-30 | 广东源控环保科技有限公司 | Sewage disposal system, the method for Hydrodynamic cavitation dephosphorization apparatus and the application device |
| CN105626595A (en) * | 2015-12-11 | 2016-06-01 | 中国航空工业集团公司金城南京机电液压工程研究中心 | Double-end jet pump |
| CN105626595B (en) * | 2015-12-11 | 2017-10-10 | 中国航空工业集团公司金城南京机电液压工程研究中心 | A kind of both-end jet pump |
| CN105758754A (en) * | 2016-02-29 | 2016-07-13 | 中国矿业大学 | Test device for cavitation erosion and abrasion |
| CN105758754B (en) * | 2016-02-29 | 2018-10-16 | 中国矿业大学 | A kind of cavitation corrosion abrasion experimental provision |
| CN105668973A (en) * | 2016-04-14 | 2016-06-15 | 湖南工业大学 | Spiral-flow type jammed cavitation device for breaking excess sludge |
| CN105668973B (en) * | 2016-04-14 | 2019-01-11 | 湖南工业大学 | A kind of spiral-flow type cracking excess sludge is jammed cavitation device |
| CN106070602A (en) * | 2016-07-01 | 2016-11-09 | 武汉大学 | One realizes sterilization of milk and homogenizing integral method and device |
| CN106172703A (en) * | 2016-07-11 | 2016-12-07 | 杨超坤 | A kind of aquatic products special bio antistaling agent process units |
| CN106136018A (en) * | 2016-07-14 | 2016-11-23 | 武汉大学 | A kind of fruit juice enzyme denaturing, sterilization and homogenizing integral method and device |
| CN106669584A (en) * | 2016-12-21 | 2017-05-17 | 武汉大学 | Intense shearing annular jet cavitation generator |
| CN107988063A (en) * | 2017-12-28 | 2018-05-04 | 广州聚能纳米生物科技股份有限公司 | A kind of split type clasmatosis structure |
| CN107988063B (en) * | 2017-12-28 | 2023-05-26 | 广州聚能纳米生物科技股份有限公司 | Split type cell disruption structure |
| CN109721637A (en) * | 2019-01-17 | 2019-05-07 | 梧州学院 | A kind of protein crushing plant |
| CN109721637B (en) * | 2019-01-17 | 2021-12-07 | 梧州学院 | Protein crushing equipment |
| CN109967429A (en) * | 2019-03-30 | 2019-07-05 | 山东大学 | One kind being used for the clean integrated device of medical instrument heating disinfection |
| CN110496415A (en) * | 2019-07-31 | 2019-11-26 | 武汉大学 | A kind of injection type cavitation extracts method, application and its device used of anthocyanin |
| CN110496415B (en) * | 2019-07-31 | 2021-01-01 | 武汉大学 | Method for extracting anthocyanin by injection type cavitation, application and device used by method |
| CN111974237A (en) * | 2020-08-10 | 2020-11-24 | 大连海事大学 | Cavitation generator with special-shaped flow channel |
| CN111974237B (en) * | 2020-08-10 | 2022-01-28 | 大连海事大学 | Cavitation generator with special-shaped flow channel |
| CN114471362A (en) * | 2022-01-29 | 2022-05-13 | 武汉大学 | Jet type super-cavitation generation system and super-cavitation generation method |
| CN114471362B (en) * | 2022-01-29 | 2023-01-10 | 武汉大学 | Jet type supercavitation generation system and supercavitation generation method |
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