CN103238723B - Preparation method of low-fluorine euphausia superb hydrolyzed protein powder - Google Patents
Preparation method of low-fluorine euphausia superb hydrolyzed protein powder Download PDFInfo
- Publication number
- CN103238723B CN103238723B CN201210029946.1A CN201210029946A CN103238723B CN 103238723 B CN103238723 B CN 103238723B CN 201210029946 A CN201210029946 A CN 201210029946A CN 103238723 B CN103238723 B CN 103238723B
- Authority
- CN
- China
- Prior art keywords
- protein
- active charcoal
- cocoanut active
- feed liquid
- content
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Meat, Egg Or Seafood Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Fodder In General (AREA)
Abstract
A preparation method of a low-fluorine euphausia superb hydrolyzed protein powder relates to processing of a euphausia superb protein powder. The preparation method provided by invention is characterized by comprising the following steps: rubbing fresh euphausia superb, adding 2 times of water, mixing, heating to 100-105 DEG C, stirring for 30 min, cooling to an enzymolysis temperature, adding protease and carrying out enzymatic hydrolysis for 2 hours, heating to 90-95 DEG C, keeping for 20 min and killing enzyme, filtering to remove shrimp shell and impurities, letting a feed liquid pass through nanofiltration membrane to be dehydrated until the protein content is 20%+/-2%, adding 4% of cocoanut active charcoal, heating to 70-80 DEG C, stirring for 15-30 min, filtering to remove cocoanut active charcoal, adding 5-7 times of deionized water to dilute a hydrolyzed protein fluid, dehydrating by the use of the nanofiltration membrane until the content of protein in the feed liquid is greater than or equal to 20%, adding 4% of cocoanut active charcoal, heating to 70-80 DEG C, stirring for 15-30 min, filtering to remove cocoanut active charcoal, carrying out vacuum concentration until the protein concentration is 35%-40%, and carrying out spray drying to prepare the dry protein powder.
Description
Technical field
The present invention relates to the processing method of krill protein powder, especially a kind of method of preparing low fluorine krill aminosal powder.
Background technology
Krill is that a kind of reserves are huge, has the protein resource of potential value of exploiting and utilizing.Its appreciable reserves are hundred million tons of 4-6.Krill protein content is high, and in muscle, protein content accounts for 64%(in dry), its amino acid content is abundant, and 8 kinds of essential amino acid proportions 45.28%, are a kind of high-quality marine animal property protein.In the marine eco-environment of krill in high latitude low temperature, in its crust transformation process, absorbing the calcium ions and magnesium ions of fluorine ion excessive in seawater in crust is combined, a large amount of fluorine is deposited among crust, after the dried up death of krill under the effect of shrimp body endoenzyme, fluorine ion penetrates among muscle, makes the fluorine content in krill muscle higher.Result of study shows krill fluorine content distribution: crust mean value 4028 μ g/g, and head (connecting feeler) mean value is 2724 μ g/g, and uropodium mean value is 2828 μ g/g, and muscle mean value is 226 μ g/g, ensemble average value is 1232 μ g/g(butts).Because the fluorine content of krill is far above China GB2762-2005 food contaminant fluorine Limited Doses, fish (fresh water) are less than the regulation of 2.0mg/kg, make the krill can not be direct edible as other marine products shrimp.Because fluorine content is higher than edible scope, the essential method of biotechnology defluorinate of passing through of utilization that makes krill, changes into water-solubility protein by the albumen of krill, removes the fluorine ion in the aqueous solution, and then be dried into protein powder, become edible albumen.Because fluorine ion is mainly present in trade effluent, carry out ion exchange with calcium chloride, with aluminium electrode enrichment fluorine ion etc. be to derive from sewage disposal to go fluorine method, among being applied to food production, can cause protein powder salt content high, the problems such as ash, content of beary metal exceed standard, do not meet requirement and the specification of food production.
Summary of the invention
The problem that the present invention need to solve is to provide one and meets food production specification, and production technology is simple and safe, is applicable to the preparation method of the krill protein powder of the low fluorine content of large-scale production.
Technical scheme of the present invention is that complete fresh krill shrimp or shrimp are rubbed and add the water of 2 times of full shrimp or shrimp weight to be uniformly mixed, be warmed to 100～105 DEG C, stir 30 minutes, cool to after 45 DEG C ± 2 DEG C protease hydrolyzed temperature, add 0.2～0.5% trypsase or papain, constant temperature enzymolysis 2 hours, then be warmed up to 20 minutes enzymes that go out of 90～95 DEG C of maintenances, filter and remove shrimp shell and impurity, feed liquid is crossed to NF membrane dewaters to protein content 20% ± 2%, add the cocoanut active charcoal of protein content 20% feed liquid weight 4%, being heated to 70～80 DEG C stirs 15～30 minutes, filter and remove cocoanut active charcoal, add the deionized water of 5 to 7 times of material liquid volumes, dilution hydrolyzed protein liquid, again dewater by NF membrane, until protein in feed liquid content >=20%, add again the cocoanut active charcoal of protein content 20% feed liquid weight 4%, being heated to 70～80 DEG C stirs 15～30 minutes, filter and remove cocoanut active charcoal, Vacuum Concentration is to protein concentration 35%-40% again, be made into protein dry powder with spray dryer.
The present invention's cocoanut active charcoal is fluorine ion adsorption aid, has safe and effective free from extraneous odour, meets the effect of food sanitation safe requirement, can't produce new salt, heavy metal and objectionable impurities.Utilize biological Nanofiltration-membrane technique that the fluorine ion in liquid is got rid of, safe and reliable, technique is simple, easy to operate, meets the krill protein Process requirement of producing low fluorine content in enormous quantities.Can obtain ash content below 5%, the krill protein powder below fluorine content 10ppm, the rate of recovery of krill muscle protein can reach 45～55%.
Brief description of the drawings
What Fig. 1 represented is process flow diagram of the present invention.
Detailed description of the invention
Get fresh krill meat, add 2 times of water 1:2 (w/v), be warmed to 100～105 DEG C, stir and keep temperature 30 minutes, be cooled to 45 DEG C ± 2 DEG C of the applicable operating temperatures of protease, add 0.2～0.5% trypsase (papain) to stir hydrolysis 2 hours, be warmed up to 90～95 DEG C of 20 minutes enzymes that go out, with plate-frame filtering removal shell and impurity, feed liquid is crossed NF membrane dehydration, reach 20% ± 2% to protein content, add 4% (W/V) cocoanut active charcoal, keep 70～80 DEG C of feed temperatures, stir 15～30 minutes, plate-frame filtering is removed cocoanut active charcoal, add the deionized water of 5 to 7 times, washing protein hydrolysate, again with biological NF membrane dehydration, until feed liquid protein content reaches 20% ± 2%, add again 4% (W/V) cocoanut active charcoal, being heated to 70～80 DEG C stirs 15～30 minutes, plate-frame filtering is removed cocoanut active charcoal, Vacuum Concentration, to protein content 35～40%, the dry protein dry powder that is made into of spraying.
Embodiment 1
(1) get fresh krill meat 1000g and be placed in reactor, the 2000g that adds water is heated with stirring to 100～105 DEG C in reactor, keeps 30 minutes.
(2) fluid temperature is down to after 45 DEG C ± 2 DEG C, adds trypsase 0.2%, constant temperature hydrolysis is stirred 2 hours, is warming up to 95 DEG C ± 2 DEG C, keeps 20min, and the enzyme that goes out is lived.
(3) emit feed liquid, with flame filter press separation slag liquid, elimination impurity and insoluble matter.
(4) feed liquid after filtering is dewatered with NF membrane GE rolling Ro film 1812, reaches 20% ± 2% to protein in feed liquid concentration.
(5) add 4%(w/v) cocoanut active charcoal, be warming up to 75 DEG C ± 2 DEG C, stir 15 minutes.
(6) feed liquid is filtered through plate filter, separates cocoanut active charcoal.
(7) in the feed liquid after filtering, slowly add 5-7 deionized water doubly. dewater to protein concentration 18-20% with NF membrane GE rolling Ro film 1812.
(8) again add 4%(w/v) cocoanut active charcoal, be warming up to 75 DEG C ± 2 DEG C, stir 15 minutes.
(9) feed liquid is filtered through plate filter, separates cocoanut active charcoal.
(10) feed liquid Vacuum Concentration, more than vacuum 0.08Mpa, 80 DEG C ± 2 DEG C of temperature, boiling is concentrated in solution protein content and stops 35～40% time.
(11) the krill albumen feed liquid after above-mentioned concentrating is sprayed and is dried, can obtain ash content below 5%, the krill protein powder below fluorine content 10ppm.
Embodiment 2
(1) get fresh krill 1000g, water 2000g, is placed on and in reactor, stirs and be heated to 105 DEG C ± 2 DEG C, and keep 30 minutes.
(2) feed liquid temperature is down to 45 DEG C ± 2 DEG C, adds the 0.2%-0.5% papain that fresh krill is heavy, constant temperature hydrolysis is stirred 2 hours.
(3) hydrolyzate is warming up to 95 DEG C ± 2 DEG C.Keep 15 minutes enzymes that go out.
(4) emit feed liquid, with centrifuge separation, the residue of getting rid of shrimp shell and not being hydrolyzed.
(5) feed liquid is dewatered once with reverse osmosis membrane, with NF membrane GE rolling Ro film 1812, is concentrated into feed liquid protein concentration 20% ± 2%.
(6) add cocoanut active charcoal 4%(w/v), be warming up to 75 DEG C ± 2 DEG C, stir 15 minutes.
(7) feed liquid is filtered with plate frame filter.
(8) in feed liquid, add the deionized water of 5～7 times to cross film, with NF membrane GE rolling Ro film 1812, be concentrated into protein concentration 20% ± 2%, get rid of waste water.
(9) in feed liquid, add 4%(w/v) cocoanut active charcoal, be warming up to 75 DEG C ± 2 DEG C, stir 15 minutes.
(10) feed liquid, through flame filter press, is got rid of cocoanut active charcoal, leaches clear liquid.
(11) Vacuum Concentration, by feed liquid more than vacuum 0.08MPa, 80 DEG C ± 2 DEG C of temperature, boiling is concentrated into protein content in feed liquid and stops in the time of 35-40%.
(12) feed liquid after concentrated is spray-dried, can obtain ash content below 5%, the krill protein powder below fluorine content 10ppm.
Claims (2)
1. the preparation method of one kind low fluorine krill aminosal powder, it is characterized in that complete fresh krill shrimp or shrimp to rub and add the water of 2 times of full shrimp or shrimp weight to be uniformly mixed, be warmed to 100～105 DEG C, stir 30 minutes, cool to after 45 DEG C ± 2 DEG C protease hydrolyzed temperature, add 0.2～0.5% trypsase or papain, constant temperature enzymolysis 2 hours, be warmed up to again 90～95 DEG C and keep 20 minutes enzymes that go out, filter and remove shrimp shell and impurity, feed liquid is crossed to NF membrane dewaters to protein content 20% ± 2%, add the cocoanut active charcoal of protein content 20% feed liquid weight 4%, being heated to 70～80 DEG C stirs 15～30 minutes, filter and remove cocoanut active charcoal, add the deionized water of 5 to 7 times of material liquid volumes, dilution hydrolyzed protein liquid, again dewater by NF membrane, until protein in feed liquid content >=20%, add again the cocoanut active charcoal of protein content 20% feed liquid weight 4%, being heated to 70～80 DEG C stirs 15～30 minutes, filter and remove cocoanut active charcoal, Vacuum Concentration is to protein concentration 35%～40% again, be made into protein dry powder with spray dryer.
2. the preparation method of a kind of low fluorine krill aminosal powder according to claim 1, is characterized in that spray-dired protein dry powder ash content is below 5%, below fluorine content 10ppm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210029946.1A CN103238723B (en) | 2012-02-10 | 2012-02-10 | Preparation method of low-fluorine euphausia superb hydrolyzed protein powder |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210029946.1A CN103238723B (en) | 2012-02-10 | 2012-02-10 | Preparation method of low-fluorine euphausia superb hydrolyzed protein powder |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103238723A CN103238723A (en) | 2013-08-14 |
| CN103238723B true CN103238723B (en) | 2014-10-29 |
Family
ID=48918868
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210029946.1A Active CN103238723B (en) | 2012-02-10 | 2012-02-10 | Preparation method of low-fluorine euphausia superb hydrolyzed protein powder |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103238723B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4091620A1 (en) * | 2018-01-30 | 2022-11-23 | Aker Biomarine Antarctic As | Krill protein hydrolysate with low fluoride and trimethylamin content |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232717A (en) * | 2014-09-12 | 2014-12-24 | 中国海洋大学 | Method for reducing fluorine content in water soluble active peptides from Antarctic krill |
| CN105248833A (en) * | 2015-10-23 | 2016-01-20 | 天津核生科技生物工程有限公司 | Method for extracting shrimp protein nutrients used for enhancing immunity and relieving fatigue |
| CN105639044A (en) * | 2016-01-22 | 2016-06-08 | 中国海洋大学 | Edible euphausia superba proteolipid compound and processing method thereof |
| CN107114605A (en) * | 2017-04-28 | 2017-09-01 | 广东越群海洋生物研究开发有限公司 | Application of the euphausia superba powder in terms of blood parrot opening material is prepared |
| CN110452946A (en) * | 2019-08-06 | 2019-11-15 | 大连工业大学 | A kind of preparation method of the krill antioxidant peptide powder of low fluorine hypoallergenic |
| CN114181988A (en) * | 2021-12-23 | 2022-03-15 | 武汉梁子湖水产品加工有限公司 | Euphausia superba meat protein peptide and preparation method thereof |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0667307B2 (en) * | 1990-02-13 | 1994-08-31 | 財団法人韓国食品開発研究院 | Antarctic shrimp weight loss method |
| CN1644073A (en) * | 2005-01-25 | 2005-07-27 | 江南大学 | Method for processing fent by comprehensive utilizing shrimp by enzyme method |
| CN1648064A (en) * | 2004-12-25 | 2005-08-03 | 李玉堂 | Water purifier for removing fluorine and arsenic and preparing method |
| CN101756147A (en) * | 2010-01-29 | 2010-06-30 | 天津商业大学 | Method for preparing low allergen shrimp base material by enzyme method |
| CN102059093A (en) * | 2009-11-18 | 2011-05-18 | 中国科学院生态环境研究中心 | Arsenic and fluorine removing nano-composite adsorbent |
| CN102170795A (en) * | 2008-09-12 | 2011-08-31 | 翡翠渔业公司 | Process for reducing the fluoride content when producing proteinaceous concentrates from krill |
| CN102342541A (en) * | 2011-09-02 | 2012-02-08 | 中国水产科学研究院东海水产研究所 | Method for reducing content of fluorine in enzymatic hydrolysate of Euphausia superb by using calcined lime |
-
2012
- 2012-02-10 CN CN201210029946.1A patent/CN103238723B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0667307B2 (en) * | 1990-02-13 | 1994-08-31 | 財団法人韓国食品開発研究院 | Antarctic shrimp weight loss method |
| CN1648064A (en) * | 2004-12-25 | 2005-08-03 | 李玉堂 | Water purifier for removing fluorine and arsenic and preparing method |
| CN1644073A (en) * | 2005-01-25 | 2005-07-27 | 江南大学 | Method for processing fent by comprehensive utilizing shrimp by enzyme method |
| CN102170795A (en) * | 2008-09-12 | 2011-08-31 | 翡翠渔业公司 | Process for reducing the fluoride content when producing proteinaceous concentrates from krill |
| CN102059093A (en) * | 2009-11-18 | 2011-05-18 | 中国科学院生态环境研究中心 | Arsenic and fluorine removing nano-composite adsorbent |
| CN101756147A (en) * | 2010-01-29 | 2010-06-30 | 天津商业大学 | Method for preparing low allergen shrimp base material by enzyme method |
| CN102342541A (en) * | 2011-09-02 | 2012-02-08 | 中国水产科学研究院东海水产研究所 | Method for reducing content of fluorine in enzymatic hydrolysate of Euphausia superb by using calcined lime |
Non-Patent Citations (1)
| Title |
|---|
| 祁鲁梁等.纳滤膜的应用.《水处理药剂及材料使用手册》.中国石化出版社,2000,(第1版),第494-495页. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4091620A1 (en) * | 2018-01-30 | 2022-11-23 | Aker Biomarine Antarctic As | Krill protein hydrolysate with low fluoride and trimethylamin content |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103238723A (en) | 2013-08-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103238723B (en) | Preparation method of low-fluorine euphausia superb hydrolyzed protein powder | |
| CN103044570B (en) | A kind of extraction process of high efficiency extraction sea grass polysaccharide | |
| CN101181080B (en) | Method for extracting crust element, fats and albumen powder from shrimp and crab | |
| CN102154420B (en) | Method for extracting pigskin collagen peptides | |
| CN102086464B (en) | Method for preparing chitin | |
| CN105385737A (en) | Preparation technology of yak bone collagen oligopeptide | |
| CN103320486B (en) | Method for producing chondroitin sulfate with coproduction of hydrolyzed collagen by employing fish cartilage | |
| CN104498568A (en) | Method for preparing fish-skin collagen powder by use of fresh fish skin | |
| CN105249464A (en) | Preparation method of shrimp peptides chelated calcium | |
| CN105366909A (en) | Sludge deep-dehydration technology | |
| CN101965898A (en) | Method for extracting peanut peptide | |
| CN103694374A (en) | Method for extracting agar, fucoidin and protein from gracilaria as raw material by using enzyme process | |
| CN104911240A (en) | Process for co-production of collagen calcium and polypeptide in chondroitin sulfate production | |
| CN102872801B (en) | Composite adsorption material for removing magnesium ions from natural water and preparation method for adsorption material | |
| CN102671630B (en) | Method for preparing printing dye adsorbent by taking eggshells as matrix and treatment method | |
| CN102732592A (en) | Method for preparing freshwater fish bone gelatin by enzyme process | |
| CN101619095B (en) | Preparation method of soybean polysaccharide protein in soybean dregs | |
| CN102940246B (en) | Prawn biological protein calcium preparation method | |
| CN102342541B (en) | Method for reducing content of fluorine in enzymatic hydrolysate of Euphausia superb by using calcined lime | |
| CN104336297A (en) | Method for extracting organic compounds of collagen and the like from chicken bones | |
| CN110050872B (en) | Industrial production method for preparing oyster peptide by enzyme method | |
| CN104292361A (en) | Method for recycling sodium alginate from reactive dye printing wastewater by virtue of calcium coagulation-chelating agent process | |
| CN104148027A (en) | Preparation method for adsorbing material for removing cadmium from slightly-polluted water body | |
| CN103695571B (en) | Subcritical water process vinasse are utilized to prepare the method for wood sugar and protolysate | |
| CN104498569B (en) | A method of collagen powder is produced using dry fish-skin |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 200433 No. 265, Jiamusi Road, Shanghai, Yangpu District Patentee after: SHANGHAI FISHERIES Research Institute SHANGHAI FISHERIES TECHNICAL EXTENSION STATION Address before: 200433 No. 265, Jiamusi Road, Shanghai, Yangpu District Patentee before: SHANGHAI FISHERIES Research Institute |
|
| CP01 | Change in the name or title of a patent holder |