CN103238723A - Preparation method of low-fluorine euphausia superb hydrolyzed protein powder - Google Patents
Preparation method of low-fluorine euphausia superb hydrolyzed protein powder Download PDFInfo
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- CN103238723A CN103238723A CN2012100299461A CN201210029946A CN103238723A CN 103238723 A CN103238723 A CN 103238723A CN 2012100299461 A CN2012100299461 A CN 2012100299461A CN 201210029946 A CN201210029946 A CN 201210029946A CN 103238723 A CN103238723 A CN 103238723A
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Abstract
A preparation method of a low-fluorine euphausia superb hydrolyzed protein powder relates to processing of a euphausia superb protein powder. The preparation method provided by invention is characterized by comprising the following steps: rubbing fresh euphausia superb, adding 2 times of water, mixing, heating to 100-105 DEG C, stirring for 30 min, cooling to an enzymolysis temperature, adding protease and carrying out enzymatic hydrolysis for 2 hours, heating to 90-95 DEG C, keeping for 20 min and killing enzyme, filtering to remove shrimp shell and impurities, letting a feed liquid pass through nanofiltration membrane to be dehydrated until the protein content is 20%+/-2%, adding 4% of cocoanut active charcoal, heating to 70-80 DEG C, stirring for 15-30 min, filtering to remove cocoanut active charcoal, adding 5-7 times of deionized water to dilute a hydrolyzed protein fluid, dehydrating by the use of the nanofiltration membrane until the content of protein in the feed liquid is greater than or equal to 20%, adding 4% of cocoanut active charcoal, heating to 70-80 DEG C, stirring for 15-30 min, filtering to remove cocoanut active charcoal, carrying out vacuum concentration until the protein concentration is 35%-40%, and carrying out spray drying to prepare the dry protein powder.
Description
Technical field
The present invention relates to the processing method of krill protein powder, especially a kind of method for preparing low fluorine krill aminosal powder.
Background technology
Krill is that a kind of reserves are huge, has the protein resource of potential value of exploiting and utilizing.Its appreciable reserves are hundred million tons of 4-6.Krill protein content height, protein content accounts for 64%(in dry in the muscle), its amino acid content is abundant, and 8 kinds of essential amino acid proportions 45.28% are a kind of high-quality marine animal property protein.Because krill is in the marine eco-environment of high latitude low temperature, in its crust transformation process, the excessive calcium ions and magnesium ions of fluorine ion in crust is combined in the absorption seawater, a large amount of fluorine is deposited among the crust, after the dried up death of krill under the effect of shrimp body endoenzyme, fluorine ion penetrates among the muscle, makes the fluorine content in the krill muscle higher.Result of study shows krill fluorine content distribution: crust mean value 4028 μ g/g, and head (connecting feeler) mean value is 2724 μ g/g, and uropodium mean value is 2828 μ g/g, and muscle mean value is 226 μ g/g, the ensemble average value is 1232 μ g/g(butts).Because the fluorine content of krill is far above China GB2762-2005 food contaminant fluorine value of limiting the quantity of, fish (fresh water) can not eat krill as other marine products shrimp is direct less than the regulation of 2.0mg/kg.Because fluorine content is higher than the edible scope, make the essential method by the biotechnology defluorinate of utilization of krill, the albumen of krill is changed into water-solubility protein, remove the fluorine ion in the aqueous solution, and then be dried into protein powder, become edible albumen.Because fluorine ion mainly is present in the trade effluent, carry out ion exchange with calcium chloride, be to derive from sewage disposal to go the fluorine method with aluminium electrode enrichment fluorine ion etc., can cause protein powder salt content height among being applied to food production, problems such as ash, content of beary metal exceed standard do not meet requirement and the standard of food production.
Summary of the invention
The problem that the present invention need solve provides a kind of food production standard that meets, and production technology is simple and safe, the preparation method of the krill protein powder of the low fluorine content of suitable large-scale production.
Technical scheme of the present invention is the full shrimp of fresh krill or shrimp to be rubbed the water that adds 2 times of full shrimp or shrimp weight mix, be warmed to 100～105 ℃, stirred 30 minutes, after cooling to 45 ℃ ± 2 ℃ protease hydrolyzed temperature, add 0.2～0.5% trypsase or papain, constant temperature enzymolysis 2 hours is warmed up to 90～95 ℃ again and keeps 20 minutes enzymes that go out, and filters and removes shrimp shell and impurity; Feed liquid is crossed NF membrane dewaters to protein content 20% ± 2%, the cocoanut active charcoal that adds protein content 20% feed liquid weight 4%, being heated to 70～80 ℃ stirred 15～30 minutes, filter and remove cocoanut active charcoal, the deionized water that adds 5 to 7 times of material liquid volumes, the dilution hydrolyzed protein liquid, dewater with NF membrane again, until protein in feed liquid content 〉=20%, the cocoanut active charcoal that adds protein content 20% feed liquid weight 4% again is heated to 70～80 ℃ and stirred 15～30 minutes, filters and removes cocoanut active charcoal, vacuum is concentrated into protein concentration 35%-40% again, is made into protein dry powder with spray dryer.
The present invention's cocoanut active charcoal is the fluorine ion adsorption aid, has safe and effective free from extraneous odour, meets the effect of food sanitation safe requirement, can't produce new salt, heavy metal and objectionable impurities.Utilize biological NF membrane technology that the fluorine ion in liquid is got rid of, safe and reliable, technology is simple, and is easy to operate, meets the krill albumen technological requirement of producing low fluorine content in enormous quantities.Can obtain ash content below 5%, the krill protein powder that fluorine content 10ppm is following, the rate of recovery of krill muscle protein can reach 45～55%.
Description of drawings
What Fig. 1 represented is process flow diagram of the present invention.
The specific embodiment
Get fresh krill meat, add 2 times of water 1:2 (w/v), be warmed to 100～105 ℃, stir and kept temperature 30 minutes, be cooled to 45 ℃ ± 2 ℃ of the suitable operating temperatures of protease, add 0.2～0.5% trypsase (papain) and stirred hydrolysis 2 hours, be warmed up to 90～95 ℃ of 20 minutes enzymes that go out, remove shell and impurity with plate-frame filtering, feed liquid is crossed the NF membrane dehydration, reach 20% ± 2% to protein content, add 4% (W/V) cocoanut active charcoal, keep 70～80 ℃ of feed temperatures, stirred 15～30 minutes, plate-frame filtering is removed cocoanut active charcoal, adds 5 to 7 times deionized water, washing protein hydrolysate, again with biological NF membrane dehydration, reach 20% ± 2% until the feed liquid protein content, add 4% (W/V) cocoanut active charcoal again, be heated to 70～80 ℃ and stirred 15～30 minutes, plate-frame filtering is removed cocoanut active charcoal, vacuum concentrates, and to protein content 35～40%, spray-drying is made into protein dry powder.
Embodiment 1
(1) gets fresh krill meat 1000g and be placed in the reactor, add water 2000g, in reactor, be heated with stirring to 100～105 ℃, kept 30 minutes.
(2) fluid temperature is down to 45 ℃ ± 2 ℃ after, add trypsase 0.2%, the constant temperature hydrolysis was stirred 2 hours, was warming up to 95 ℃ ± 2 ℃, kept 20min, the enzyme that goes out is lived.
(3) emit feed liquid, separate slag liquid, elimination impurity and insoluble matter with flame filter press.
(4) feed liquid after filtering is with 1812 dehydrations of NF membrane GE rolling Ro film, reaches 20% ± 2% to protein in feed liquid concentration.
(5) cocoanut active charcoal adding 4%(w/v) is warming up to 75 ℃ ± 2 ℃, stirs 15 minutes.
(6) feed liquid is filtered through plate filter, separates cocoanut active charcoal.
(7) slowly add 5-7 deionized water doubly in the feed liquid after the filtration. dewater to protein concentration 18-20% with NF membrane GE rolling Ro film 1812.
(8) add 4%(w/v again) cocoanut active charcoal, be warming up to 75 ℃ ± 2 ℃, stirred 15 minutes.
(9) feed liquid is filtered through plate filter, separates cocoanut active charcoal.
(10) the feed liquid vacuum concentrates, more than the vacuum 0.08Mpa, and 80 ℃ ± 2 ℃ of temperature, boiling is concentrated into that protein content stopped at 35～40% o'clock in the solution.
(11) the krill albumen feed liquid after above-mentioned the concentrating is carried out spray-drying, can obtain ash content below 5%, the krill protein powder that fluorine content 10ppm is following.
Embodiment 2
(1) get fresh krill 1000g, water 2000g is placed in the reactor and stirs and be heated to 105 ℃ ± 2 ℃, and kept 30 minutes.
(2) feed liquid body temperature degree is down to 45 ℃ ± 2 ℃, adds the heavy 0.2%-0.5% papain of fresh krill, the constant temperature hydrolysis was stirred 2 hours.
(3) hydrolyzate is warming up to 95 ℃ ± 2 ℃.Keep 15 minutes enzymes that go out.
(4) emit feed liquid, separate with centrifuge, the residue of getting rid of the shrimp shell and not being hydrolyzed.
(5) feed liquid with the reverse osmosis membrane dehydration once with NF membrane GE rolling Ro film 1812, is concentrated into feed liquid protein concentration 20% ± 2%.
(6) add cocoanut active charcoal 4%(w/v), be warming up to 75 ℃ ± 2 ℃, stirred 15 minutes.
(7) feed liquid is filtered with plate frame filter.
(8) deionized water that adds 5～7 times in the feed liquid is crossed film, with NF membrane GE rolling Ro film 1812, is concentrated into protein concentration 20% ± 2%, gets rid of waste water.
(9) add 4%(w/v in the feed liquid) cocoanut active charcoal, be warming up to 75 ℃ ± 2 ℃, stirred 15 minutes.
(10) feed liquid is got rid of cocoanut active charcoal through flame filter press, leaches clear liquid.
(11) vacuum concentrates, with feed liquid more than vacuum 0.08MPa, 80 ℃ ± 2 ℃ of temperature, boiling is concentrated into that protein content stops in the feed liquid when 35-40%.
(12) feed liquid after concentrating is spray-dried, can obtain ash content below 5%, the krill protein powder that fluorine content 10ppm is following.
Claims (2)
1. the preparation method of one kind low fluorine krill aminosal powder, it is characterized in that the full shrimp of fresh krill or shrimp are rubbed the water that adds 2 times of full shrimp or shrimp weight to mix, be warmed to 100～105 ℃, stirred 30 minutes, after cooling to 45 ℃ ± 2 ℃ protease hydrolyzed temperature, add 0.2～0.5% trypsase or papain, constant temperature enzymolysis 2 hours, be warmed up to 90～95 ℃ again and keep 20 minutes enzymes that go out, filter and remove shrimp shell and impurity. feed liquid is crossed NF membrane dewater to protein content 20% ± 2%, the cocoanut active charcoal that adds protein content 20% feed liquid weight 4%, being heated to 70～80 ℃ stirred 15～30 minutes, filter and remove cocoanut active charcoal, the deionized water that adds 5 to 7 times of material liquid volumes, the dilution hydrolyzed protein liquid, dewater with NF membrane again, until protein in feed liquid content 〉=20%, the cocoanut active charcoal that adds protein content 20% feed liquid weight 4% again, being heated to 70～80 ℃ stirred 15～30 minutes, filter and remove cocoanut active charcoal, vacuum is concentrated into protein concentration 35%～40% again, is made into protein dry powder with spray dryer.
2. the preparation method of a kind of low fluorine krill aminosal powder according to claim 1 is characterized in that spray-dired protein dry powder ash content below 5%, below the fluorine content 10ppm.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232717A (en) * | 2014-09-12 | 2014-12-24 | 中国海洋大学 | Method for reducing fluorine content in water soluble active peptides from Antarctic krill |
| CN105248833A (en) * | 2015-10-23 | 2016-01-20 | 天津核生科技生物工程有限公司 | Method for extracting shrimp protein nutrients used for enhancing immunity and relieving fatigue |
| CN105639044A (en) * | 2016-01-22 | 2016-06-08 | 中国海洋大学 | Edible euphausia superba proteolipid compound and processing method thereof |
| CN107114605A (en) * | 2017-04-28 | 2017-09-01 | 广东越群海洋生物研究开发有限公司 | Application of the euphausia superba powder in terms of blood parrot opening material is prepared |
| WO2019150197A1 (en) | 2018-01-30 | 2019-08-08 | Aker Biomarine Antarctic As | Marine protein hydrolysate with low fluoride and trimethylamin content |
| CN110452946A (en) * | 2019-08-06 | 2019-11-15 | 大连工业大学 | A kind of preparation method of the krill antioxidant peptide powder of low fluorine hypoallergenic |
| CN114181988A (en) * | 2021-12-23 | 2022-03-15 | 武汉梁子湖水产品加工有限公司 | Euphausia superba meat protein peptide and preparation method thereof |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232717A (en) * | 2014-09-12 | 2014-12-24 | 中国海洋大学 | Method for reducing fluorine content in water soluble active peptides from Antarctic krill |
| CN105248833A (en) * | 2015-10-23 | 2016-01-20 | 天津核生科技生物工程有限公司 | Method for extracting shrimp protein nutrients used for enhancing immunity and relieving fatigue |
| CN105639044A (en) * | 2016-01-22 | 2016-06-08 | 中国海洋大学 | Edible euphausia superba proteolipid compound and processing method thereof |
| CN107114605A (en) * | 2017-04-28 | 2017-09-01 | 广东越群海洋生物研究开发有限公司 | Application of the euphausia superba powder in terms of blood parrot opening material is prepared |
| WO2019150197A1 (en) | 2018-01-30 | 2019-08-08 | Aker Biomarine Antarctic As | Marine protein hydrolysate with low fluoride and trimethylamin content |
| EP3749339B1 (en) * | 2018-01-30 | 2022-08-03 | Aker Biomarine Antarctic As | Marine protein hydrolysate with low fluoride and trimethylamin content |
| CN110452946A (en) * | 2019-08-06 | 2019-11-15 | 大连工业大学 | A kind of preparation method of the krill antioxidant peptide powder of low fluorine hypoallergenic |
| CN114181988A (en) * | 2021-12-23 | 2022-03-15 | 武汉梁子湖水产品加工有限公司 | Euphausia superba meat protein peptide and preparation method thereof |
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| CN103238723B (en) | 2014-10-29 |
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Address after: 200433 No. 265, Jiamusi Road, Shanghai, Yangpu District Patentee after: SHANGHAI FISHERIES Research Institute SHANGHAI FISHERIES TECHNICAL EXTENSION STATION Address before: 200433 No. 265, Jiamusi Road, Shanghai, Yangpu District Patentee before: SHANGHAI FISHERIES Research Institute |