CN103238629B - Cold-resistant agent for growth of Chinese trichosanthes and using method thereof - Google Patents
Cold-resistant agent for growth of Chinese trichosanthes and using method thereof Download PDFInfo
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- CN103238629B CN103238629B CN201310158886.8A CN201310158886A CN103238629B CN 103238629 B CN103238629 B CN 103238629B CN 201310158886 A CN201310158886 A CN 201310158886A CN 103238629 B CN103238629 B CN 103238629B
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- acid
- snakegourd
- cold
- resistant agent
- putrescine
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- 230000012010 growth Effects 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 8
- 241000218989 Trichosanthes Species 0.000 title abstract 8
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 75
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims abstract description 72
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 claims abstract description 60
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 claims abstract description 56
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 claims abstract description 52
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 50
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims abstract description 36
- 229960004889 salicylic acid Drugs 0.000 claims abstract description 36
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 35
- 239000005700 Putrescine Substances 0.000 claims abstract description 30
- 229940063673 spermidine Drugs 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
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- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims abstract description 25
- 239000004202 carbamide Substances 0.000 claims abstract description 25
- 239000001630 malic acid Substances 0.000 claims abstract description 25
- 235000011090 malic acid Nutrition 0.000 claims abstract description 25
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 14
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 14
- 244000078912 Trichosanthes cucumerina Species 0.000 claims description 67
- 235000008326 Trichosanthes anguina Nutrition 0.000 claims description 65
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- 229910002651 NO3 Inorganic materials 0.000 claims description 26
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- 239000004571 lime Substances 0.000 claims description 26
- 238000003756 stirring Methods 0.000 claims description 14
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 13
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- 239000007921 spray Substances 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 5
- -1 shitosan Chemical compound 0.000 claims description 3
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- 230000006378 damage Effects 0.000 abstract description 8
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- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 abstract 2
- 229920001661 Chitosan Polymers 0.000 abstract 1
- 210000000170 cell membrane Anatomy 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 15
- 241000196324 Embryophyta Species 0.000 description 9
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- 230000000694 effects Effects 0.000 description 6
- JLIDBLDQVAYHNE-LXGGSRJLSA-N 2-cis-abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\C1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-LXGGSRJLSA-N 0.000 description 5
- 240000006023 Trichosanthes kirilowii Species 0.000 description 5
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- 208000027418 Wounds and injury Diseases 0.000 description 5
- 208000014674 injury Diseases 0.000 description 5
- 241000219112 Cucumis Species 0.000 description 4
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 4
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
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- 230000002595 cold damage Effects 0.000 description 3
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- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
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- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
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- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
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- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
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- 208000037386 Typhoid Diseases 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
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- 239000003513 alkali Substances 0.000 description 1
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- QVYARBLCAHCSFJ-UHFFFAOYSA-N butane-1,1-diamine Chemical compound CCCC(N)N QVYARBLCAHCSFJ-UHFFFAOYSA-N 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a cold-resistant agent for growth of Chinese trichosanthes. The cold-resistant agent comprises the following components by weight: 5-7.5mg of abscisic acid, 0.03-0.06g of salicylic acid, 0.6-1g of calcium nitrate, 0.035-0.04g of putrescine, 0.05g of spermidine, 0.45g of urea, 0.3g of monopotassium phosphate, 0.04g of citric acid, 0.1g of malic acid, 0.05g of chitosan, and 1L of water. The invention simultaneously discloses a using method of the cold-resistant agent for growth of Chinese trichosanthes. The method comprises the following steps of: spraying leaves of a Chinese trichosanthes plant by a Chinese trichosanthes cold-resistant agent until the leaves drip 12-48 hours before a cold wave comes. By adopting the cold-resistant agent for growth of the Chinese trichosanthes disclosed by the invention, the ability of the Chinese trichosanthes for resisting low-temperature damage can be obviously enhanced; and the stability of a Chinese trichosanthes cell membrane also can be enhanced.
Description
Technical field
The present invention relates to a kind of cold-resistance spraying agent, refer to especially a kind of cold-resistance agent that improves snakegourd cold tolerance.
Background technology
Damaging to plants caused by sudden drop in temperature (chilling damage) is a kind of of agrometeorological hazard.Damage to plants caused by sudden drop in temperature and make plant physiology activity be subject to obstacle, when serious, some tissue is destroyed.Along with the deterioration of ecotope, global climate has extremely become the huge hidden danger of crop production reduction, area of woods minimizing, animals and plants in imminent danger extinction.Recent years, La Nina's weather entered China, and the cooling Abnormal Precipitation bringing thus has caused huge economic loss.At the beginning of 2008, owing to being subject to the impact of La Nina climatic phenomenon, China's drug in some provinces region temperature sharply declines, and temperature is lower, and the duration is longer, and involvement aspect is wider, and the extent of injury is quite large.The whole world is every year because low temperature cold damage causes the loss of many crops up to hundreds billion of dollars.
Snakegourd (Trichosathes kirilowii Maxim), another name melon withers, melon building, medicine melon, hangs melon, is Curcurbitaceae herbaceous perennial vine, and dioecism, has medicinal and edible two large functions concurrently.Seeds of Trichosanthes kirilowii is often fried rear edible, because it is rich in various trace elements and the special fatty acid such as fat, protein, vitamin E and calcium, magnesium, potassium, iron, zinc of a large amount of needed by human body, Seeds of Trichosanthes kirilowii has the function of moistening lung for removing phlegm, clearing heat for detumescence, relieving chest stiffness to dissipate mass, laxation defaecation, often proper amount of edible, has good health-care effect to health; Snakegourd piece root is containing " trichosanthin ", the effects such as that trichosanthin has is antitumor, anti-AIDS; In pericarp, contain snakegourd ester alkali, there is the effects such as pain relieving spasmolysis, treatment hepatitis; In snakegourd fruit, uranidin is a kind of safe natural colouring matter, can be used as natural, but also has certain health-care effect, is expected to become a kind of functional pigmentedly use in numerous food.Snakegourd has high exploitation as economic crops and is worth, at present, snakegourd industry starts flourish at our province, but meanwhile, also show successively aborning some larger economic problems, especially day and night temperature is larger, and the aggravation of climatic anomaly certainly will cause certain loss to the plant husbandry of snakegourd.Therefore, in the face of weather mutation, winter low temperature sleet phase extend this harsh fact that is unfavorable for plant growth to some extent, should carry out aborning to defend is main counter-measure, and the prevention work of carrying out in advance crop protection against cold and freezing conditions is very important.In the cultivation management of snakegourd, Rational Application cold-resistance agent is the major measure that realizes its high yield matter, yet owing to lacking cold-resistance agent formula targetedly, has the not good enough problem of result of use.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of snakegourd growth cold resistant agent that can obviously strengthen snakegourd opposing injury from low temperature, and this snakegourd growth cold resistant agent can strengthen the stability of Cells in Trichosanthes kirilowii Maxim film.
In order to solve the problems of the technologies described above, the invention provides a kind of snakegourd growth cold resistant agent, its one-tenth by following content is grouped into: abscisic acid (ABA) 5 ~ 7.5 mg, salicylic acid (SA) 0.03 ~ 0.06 g, nitrate of lime (Ca(NO
3)
2) 0.6 ~ 1 g, putrescine 0.035 ~ 0.04 g, spermidine 0.05 g, urea 0.45g, potassium dihydrogen phosphate (K
2hPO
4) 0.3 g, citric acid 0.04 g, malic acid 0.1 g, shitosan 0.05 g; Water 1L.
As the improvement of snakegourd growth cold resistant agent of the present invention, its one-tenth by following content is grouped into: abscisic acid 0.0075g, salicylic acid 0.06g, nitrate of lime 1g, putrescine 0.04g, spermidine 0.05g, urea 0.45g, KH
2pO
40.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05 g, water 1L.
The present invention also provides another kind of snakegourd growth cold resistant agent simultaneously, and its one-tenth by following content is grouped into: abscisic acid (ABA) 5 ~ 7.5 mg, salicylic acid (SA) 0.03 ~ 0.06 g, nitrate of lime (Ca(NO
3)
2) 0.6 ~ 1 g, putrescine 0.035 ~ 0.04 g, spermidine 0.05 g, urea 0.45g, potassium dihydrogen phosphate (K
2hPO
4) 0.3 g, citric acid 0.04 g, malic acid 0.1 g, shitosan 0.05 g, Difenoconazole 0.014 ~ 0.017g; Water 1L.
The present invention also provides the using method of above-mentioned 2 class snakegourd growth cold resistant agents simultaneously, comprise the following steps: at the front 12 ~ 48h of cold wave arriving, with snakegourd cold-resistance agent, spray the blade of snakegourd plant, until snakegourd growth cold resistant agent naturally flows down (that is, till blade dropping liquid) to blade.Use the object of snakegourd growth cold resistant agent to be: to strengthen the resistivity of snakegourd to low temperature, reduce the economic loss that cold damage brings.
Putrescine of the present invention is butanediamine, putrescine, H
2n(CH
2)
4nH
2.
Generally, when the residing environmental temperature of snakegourd is during lower than 10 ℃, just need to use in the manner described above snakegourd growth cold resistant agent of the present invention.
In snakegourd growth cold resistant agent of the present invention, add 0.014 ~ 0.017g/L Difenoconazole, in cold-resistant, can also effectively prevent snakegourd anthracnose.
Snakegourd growth cold resistant agent preparation method of the present invention is very easy, and preparation process is as follows:
1,, according to formula, take abscisic acid (ABA), salicylic acid (SA), nitrate of lime (Ca(NO
3)
2), putrescine, spermidine, urea, potassium dihydrogen phosphate (K
2hPO
4), citric acid, malic acid, shitosan, Difenoconazole;
2, in 1L water, add salicylic acid (SA) and abscisic acid (ABA), be heated to 40 ~ 60 ℃ and stir (object is to impel the dissolving in water of salicylic acid and abscisic acid); After salicylic acid (SA) and abscisic acid (ABA) dissolving, add nitrate of lime (Ca(NO
3)
2), potassium dihydrogen phosphate (K
2hPO
4), urea stirs; Treat nitrate of lime (Ca(NO
3)
2), potassium dihydrogen phosphate (K
2hPO
4), after urea dissolves, then add putrescine, spermidine to stir; After putrescine, spermidine dissolve, then add citric acid, malic acid, shitosan, Difenoconazole to carry out uniform stirring, thereby obtain snakegourd growth cold resistant agent.
Remarks explanation: while not containing Difenoconazole in formula, all do not comprise Difenoconazole in above-mentioned steps 1 and step 2.
Adopt snakegourd growth cold resistant agent of the present invention, can strengthen the resistivity of snakegourd to low temperature, reduce the economic loss that cold damage brings.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 is the affect comparison diagram of the snakegourd growth cold resistant agent of each embodiment gained on snakegourd MDA content under low temperature stress;
Note: contrast-contrast (CK), sprays clear water; 1-embodiment 1; 2-embodiment 2; 9-embodiment 9.
Fig. 2 is the affect comparison diagram of the snakegourd growth cold resistant agent of each embodiment gained on Cells in Trichosanthes kirilowii Maxim membrane permeability under low temperature stress;
Note: contrast-contrast (CK), sprays clear water; 1-embodiment 1; 2-embodiment 2; 9-embodiment 9.
The affect comparison diagram of the snakegourd growth cold resistant agent of each comparative example of Fig. 3 and embodiment 8 gained on snakegourd MDA content under low temperature stress;
Note: 1-embodiment 8; 2-comparative example 1; 3-comparative example 2; 12-comparative example 11.
The affect comparison diagram of each comparative example of Fig. 4 on Cells in Trichosanthes kirilowii Maxim membrane permeability under low temperature stress;
Note: 1-embodiment 8; 2-comparative example 1; 3-comparative example 2; 12-comparative example 11.
Embodiment
Embodiment 1, a kind of snakegourd cold-resistance agent, be grouped into by the one-tenth of following content: abscisic acid 0.005g, salicylic acid 0.03g, nitrate of lime 0. 6g, putrescine 0.035g, spermidine 0.05g, urea 0.45g, KH
2pO
40.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05 g, water 1L.
Embodiment 2, a kind of snakegourd cold-resistance agent, be grouped into by the one-tenth of following content: abscisic acid 0.005g, salicylic acid 0.03g, nitrate of lime 1g, putrescine 0.04g, spermidine 0.05g, urea 0.45g, KH
2pO
40.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05 g, water 1L.
Embodiment 3, a kind of snakegourd cold-resistance agent, be grouped into by the one-tenth of following content: abscisic acid 0.005g, salicylic acid 0.06g, nitrate of lime 0. 6g, putrescine 0.035g, spermidine 0.05g, urea 0.45g, KH
2pO
40.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05 g, water 1L.
Embodiment 4, a kind of snakegourd cold-resistance agent, be grouped into by the one-tenth of following content: abscisic acid 0.005g, salicylic acid 0.06g, nitrate of lime 1g, putrescine 0.035g, spermidine 0.05g, urea 0.45g, KH
2pO
40.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05 g, water 1L.
Embodiment 5, a kind of snakegourd cold-resistance agent, be grouped into by the one-tenth of following content: abscisic acid 0.0075g, salicylic acid 0.03g, nitrate of lime 0.6g, putrescine 0.035g, spermidine 0.05g, urea 0.45g, KH
2pO
40.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05 g, water 1L.
Embodiment 6, a kind of snakegourd cold-resistance agent, be grouped into by the one-tenth of following content: abscisic acid 0.0075g, salicylic acid 0.03g, nitrate of lime 1g, putrescine 0.04g, spermidine 0.05g, urea 0.45g, KH
2pO
40.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05 g, water 1L.
Embodiment 7, a kind of snakegourd cold-resistance agent, be grouped into by the one-tenth of following content: abscisic acid 0.0075g, salicylic acid 0.06g, nitrate of lime 0. 6g, putrescine 0.035g, spermidine 0.05g, urea 0.45g, KH
2pO
40.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05 g, water 1L.
Embodiment 8, a kind of snakegourd cold-resistance agent, be grouped into by the one-tenth of following content: abscisic acid 0.0075g, salicylic acid 0.06g, nitrate of lime 1g, putrescine 0.04g, spermidine 0.05g, urea 0.45g, KH
2pO
40.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05 g, water 1L.
Embodiment 9, a kind of snakegourd cold-resistance agent, be grouped into by the one-tenth of following content: abscisic acid 0.005g, salicylic acid 0.06g, nitrate of lime 0.6g, putrescine 0.04g, spermidine 0.05g, urea 0.45g, KH
2pO
40.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05 g, Difenoconazole 0.014g; Water 1L.
Experiment 1, in order to verify the cold performance of snakegourd growth cold resistant agent of the present invention, using the snakegourd growth cold resistant agent of above-described embodiment 1 ~ embodiment 9 gained respectively as 9 experimental group, to spray clear water as a control group; Carried out following specific experiment (each group arranges 3 repetitions, and each repeats to arrange 3 strain snakegourd seedlings):
Experimental group: carry out before low temperature stress is processed till 24h sprays snakegourd growth cold resistant agent to snakegourd blade and have water droplet drippage, spraying again once after 24h at snakegourd, then carry out immediately 4 ℃ of low temperature stress processing (processing time is 5d);
Control group: substitute snakegourd growth cold resistant agent with clear water; All the other are equal to;
1) the snakegourd blade of, low temperature stress being processed after 5d adopts sulfo-barbital method to carry out the detection of Lipid peroxidation (MDA content); Concrete outcome as shown in Figure 1.
Remarks explanation: MDA is one of primary product of film lipid peroxidation, its content height can react the injured degree of plant membrane system.MDA is malonaldehyde, and in organism, Free Radical is in lipid generation peroxidization, oxidation end-product is malonaldehyde, and the height of MDA content is commonly used for the index of the undermined degree of identification of cell film on biology, MDA content is larger, illustrates that the plant degree that is hurt is larger.
By Fig. 1, can find out and use after snakegourd growth cold resistant agent of the present invention, obviously reduce the MDA content in plant cell.
2) the snakegourd blade of, low temperature stress being processed after 5d adopts electrical conductivity method to carry out the detection of film fat phase transformation; Concrete outcome as shown in Figure 2.
Remarks explanation: plant is when the injury from low temperature suffering above zero, and the phase transformation of film fat can occur biomembrane, and film is shunk, and occurs duct or be full of cracks on film, thereby membrane permeability increases.Therefore cell leakage can be used as judgement plant index by typhoid.
Used as seen from Figure 2 after snakegourd growth cold resistant agent of the present invention, the cell leakage of snakegourd obviously reduces.
Comparative example 1,
" abscisic acid 0.0075g, salicylic acid 0.06g " in embodiment 8 made into " abscisic acid 0.0675g ", and all the other are with embodiment 8.
Comparative example 2,
" abscisic acid 0.0075g, salicylic acid 0.06g " in embodiment 8 made into " salicylic acid 0.0675g ", and all the other are with embodiment 8.
Comparative example 3,
" citric acid 0.04g, malic acid 0.1g " in embodiment 8 made into " citric acid 0.14g ", and all the other are with embodiment 8.
Comparative example 4,
" citric acid 0.04g, malic acid 0.1g " in embodiment 8 made into " malic acid 0.14g ", and all the other are with embodiment 8.
Comparative example 5,
" putrescine 0.04g, spermidine 0.05g " in embodiment 8 made into " putrescine 0.09g ", and all the other are with embodiment 8.
Comparative example 6,
" putrescine 0.04g, spermidine 0.05g " in embodiment 8 made into " spermidine 0.09g ", and all the other are with embodiment 8.
Comparative example 7,
" nitrate of lime 1g " in embodiment 8 made into " superphosphate 1g ", and all the other are with embodiment 8.
Comparative example 8,
" nitrate of lime 1g " in embodiment 8 made into " calcium chloride 1g ", and all the other are with embodiment 8.
Comparative example 9,
By " the KH in embodiment 8
2pO
40.3g " make " potassium chloride 0.3g " into, all the other are with embodiment 8.
Comparative example 10,
By " the KH in embodiment 8
2pO
40.3g " make " potassium nitrate 0.3g " into, all the other are with embodiment 8.
Comparative example 11,
By " the KH in embodiment 8
2pO
40.3g " make " potassium sulfate 0.3g " into, all the other are with embodiment 8.
Comparative example result of implementation is shown in Fig. 3 and Fig. 4.
From Fig. 3 and Fig. 4, the cold-resistant successful of above-mentioned comparative example is cold-resistant effective not as embodiment's 8.
By above-mentioned experimental data, can be found out, spray cold-resistance agent, can obviously reduce the degree that snakegourd is subject to injury from low temperature.
Result by above-mentioned example can be seen, uses the cold-resistant preparation of plant growth of the present invention can effectively strengthen the cold resistance of snakegourd, can reduce the injury effect of cold wave to snakegourd.
Finally, it is also to be noted that, what more than enumerate is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, can also have many distortion.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (4)
1. snakegourd growth cold resistant agent, is characterized in that being grouped into by the one-tenth of following content: abscisic acid 5～7.5mg, salicylic acid 0.03～0.06g, nitrate of lime 0.6～1g, putrescine 0.035～0.04g, spermidine 0.05g, urea 0.45g, potassium dihydrogen phosphate 0.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05g; Water 1L;
Preparation method is for carrying out successively following steps:
1), according to above-mentioned formula, take abscisic acid, salicylic acid, nitrate of lime, putrescine, spermidine, urea, potassium dihydrogen phosphate, citric acid, malic acid and shitosan;
2), in 1L water, add salicylic acid and abscisic acid, be heated to 40～60 ℃ and stir, the object of stirring is to impel the dissolving in water of salicylic acid and abscisic acid; After salicylic acid and abscisic acid dissolving, add nitrate of lime, potassium dihydrogen phosphate, urea to stir; After nitrate of lime, potassium dihydrogen phosphate, urea dissolve, then add putrescine, spermidine to stir; After putrescine, spermidine dissolve, then add citric acid, malic acid, shitosan to carry out uniform stirring, thereby obtain snakegourd growth cold resistant agent.
2. snakegourd growth cold resistant agent according to claim 1, is characterized in that being grouped into by the one-tenth of following content: abscisic acid 0.0075g, salicylic acid 0.06g, nitrate of lime 1g, putrescine 0.04g, spermidine 0.05g, urea 0.45g, KH
2pO
40.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05g, water 1L.
3. snakegourd growth cold resistant agent, it is characterized in that being grouped into by the one-tenth of following content: abscisic acid 5～7.5mg, salicylic acid 0.03～0.06g, nitrate of lime 0.6～1g, putrescine 0.035～0.04g, spermidine 0.05g, urea 0.45g, potassium dihydrogen phosphate 0.3g, citric acid 0.04g, malic acid 0.1g, shitosan 0.05g, Difenoconazole 0.014～0.017g; Water 1L;
Preparation method is for carrying out successively following steps:
1), according to above-mentioned formula, take abscisic acid, salicylic acid, nitrate of lime, putrescine, spermidine, urea, potassium dihydrogen phosphate, citric acid, malic acid, shitosan and Difenoconazole;
2), in 1L water, add salicylic acid and abscisic acid, be heated to 40～60 ℃ and stir, the object of stirring is to impel the dissolving in water of salicylic acid and abscisic acid; After salicylic acid and abscisic acid dissolving, add nitrate of lime, potassium dihydrogen phosphate, urea to stir; After nitrate of lime, potassium dihydrogen phosphate, urea dissolve, then add putrescine, spermidine to stir; After putrescine, spermidine dissolve, then add citric acid, malic acid, shitosan, Difenoconazole to carry out uniform stirring, thereby obtain snakegourd growth cold resistant agent.
4. the using method of the snakegourd growth cold resistant agent as described in claim 1,2 or 3, is characterized in that comprising the following steps:
At the front 12～48h of cold wave arriving, with snakegourd cold-resistance agent, spray the blade of snakegourd plant, until blade dropping liquid.
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| US20230024123A1 (en) * | 2017-09-21 | 2023-01-26 | Valent Biosciences Llc | Methods of improving stress tolerance, growth and yield in plants |
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| CN1163145C (en) * | 1997-10-05 | 2004-08-25 | 中国科学院沈阳应用生态研究所 | Cold resistant agent for Chinese chestnut growth |
| CN1358432A (en) * | 2000-12-14 | 2002-07-17 | 中国科学院黑龙江农业现代化研究所 | Cold-resistance agent for soybean |
| CN102887786A (en) * | 2012-04-27 | 2013-01-23 | 中国计量学院 | Natural cold-resistant agent for tea trees and preparation method for natural cold-resistant agent |
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