CN102887786A - Natural cold-resistant agent for tea trees and preparation method for natural cold-resistant agent - Google Patents

Natural cold-resistant agent for tea trees and preparation method for natural cold-resistant agent Download PDF

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CN102887786A
CN102887786A CN 201210134463 CN201210134463A CN102887786A CN 102887786 A CN102887786 A CN 102887786A CN 201210134463 CN201210134463 CN 201210134463 CN 201210134463 A CN201210134463 A CN 201210134463A CN 102887786 A CN102887786 A CN 102887786A
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cold
tea tree
preparation
resistance agent
bamboo vinegar
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黄伟峰
商晗武
高永生
胡剑光
余秋珠
王道泽
邵宝富
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China Jiliang University
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China Jiliang University
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Abstract

The invention discloses a preparation capable of improving the cold resistance of tea trees and a preparation method for the preparation. Late spring cold greatly affects the production of tea, but an effective cold-resistant agent which ensures that the tea trees are free from freezing injury is not invented at present. The preparation mainly comprises bamboo vinegar, physiological cold-resistant substances, a growth promotion substance, an acidity regulator and the like. The preparation method comprises the following steps of: 1, taking the required bamboo vinegar in a proportion, adding a lot of trace elements which are required to be mixed, and stirring until the trace elements are dissolved; 2, dissolving physiological cold-resistant substances which are required to be mixed such as abscisic acid into absolute ethanol; 3, mixing a solution obtained in step 1 and a solution obtained in step 2, adding a proper amount of acidity regulator, regulating until the PH value is appropriate, stirring until the acidity regulator is dissolved, adding other physiological cold-resistant substances, and stirring until the physiological cold-resistant substances are dissolved; 4, dissolving a growth hormone which is required to be mixed into hot absolute ethanol, and fixing the volume by using the bamboo vinegar; and 5, mixing a solution obtained in step 3 and a solution obtained in step 4, and uniformly blending to obtain the preparation. The preparation is characterized in that a natural organic substance is taken as a main raw material, and the preparation can be safely used, does not pollute the environment, and can remarkably improve the cold resistance of the tea trees.

Description

A kind of natural tea tree cold-resistance agent and preparation method thereof
Technical field
The present invention relates to a kind of cold-resistance agent, especially a kind of natural medicine that improves the tea tree cold tolerance and preparation method thereof.
Background technology
In Tea Production, annual cold spell in spring gently then makes the tealeaves underproduction, degrades, and is heavy then cause tealeaves total crop failure, brings immeasurable financial loss to tea district tea grower, thereby affects the development of Tea Industry.Especially the economic benefit of a tea and two tea differs greatly, and cold spell in later spring has a strong impact on the production of a tea.Therefore the freeze proof problem of winter protection of tealeaves is the problem that numerous tea growers will face every year, so can solve the technical products that improves the tealeaves tolerance to cold, is a kind of product with remarkable Social benefit and economic benefit.
At present, the method for minimizing tea tree cold damage mainly contains the following aspects: 1, introduce a fine variety and the seed selection improved tea variety; 2, strengthen cultivating and managing; 3, physical method protection; 4, chemical process protection.Wherein the physical method protection is present most widely used means to prevent freezing, its center be around heat, the aspect such as windproof carries out, major measure arranges breakwind, it is antifreeze to litter, fumigation is freeze proof etc., but these methods need to be used more material and manpower, have increased tea grower's production cost.
The present invention is take bamboo vinegar liquid as matrix, adds physiology cryoprotectant, growth helping matter, acidity regulator etc., forms with the technical process proportioning according to a certain percentage.This cold-resistance agent has overcome the drawbacks such as existing cold-resistance agent active constituent content is single, proportioning simple, the production technique shortage is scientific in conjunction with the variation of tea tree physiological characteristic.
Summary of the invention
Technical problem to be solved by this invention provide a kind of take natural organic substance as main raw material, use safe, free from environmental pollution, can obviously improve preparation of tea tree cold tolerance and preparation method thereof.
The present invention addresses the above problem the technical scheme that adopts: this tea tree cold-resistance agent contains bamboo vinegar liquid, physiology cryoprotectant, growth helping matter, acidity regulator etc.
Wherein the weight percent of bamboo vinegar liquid is 75-85%, the weight percent of physiology cryoprotectant is 8-14%, the weight percent of growth helping matter is 6-9%, and the weight percent of acidity regulator is 0.5-1%, and the weight percent of other auxiliary agent such as wetting Agent for Printing Inks solubilizing agent etc. is 0.5-1%.
This tea tree cold-resistance agent can also add wetting Agent for Printing Inks, and described wetting Agent for Printing Inks is animal/vegetable protein glue.
This tea tree cold-resistance agent can also add solubilizing agent, and described solubilizing agent is tween-80, dehydrated alcohol.
Physiology cryoprotectant of the present invention can be a kind of of rare earth element, dormin, carbohydrate, Whitfield's ointment, organonitrogen, organic calcium or more than one; Described growth helping matter can be a kind of of Macro and microelements and tethelin or more than one; Described acidity regulator is that calcium hydroxide is or/and potassium hydroxide.
Its rare earth elements can be Lanthanum trichloride or Cerium II Chloride.
Wherein carbohydrate can be sucrose, glucose, lactose or wood sugar.
Wherein organonitrogen can be animal/vegetable protein glue or each seed amino acid, and wherein animal/vegetable protein glue can be gelatine, gelatin, agar or natural resin.
Wherein organic calcium can be citrate of lime, calglucon or calcium lactate.
What wherein Macro and microelements can be for potassium primary phosphate, ammonium molybdate, magnesium chloride, zinc chloride is a kind of or whole.
Wherein tethelin can be a kind of of Plant hormones regulators,gibberellins, phytokinin, triacontanol price quote and other plant endogenous hormones or more than one.
Preparation method of the present invention is divided into following step.
Step 1: get in proportion required bamboo vinegar liquid, the Macro and microelements that adding need be joined is stirred to dissolving.Be designated as solution A.
Step 2: the physiology cryoprotectant that need join is dissolved in the dehydrated alcohol.Be designated as solution B.
Step 3: the tethelin that need join is dissolved in the dehydrated alcohol of heat, uses the bamboo vinegar liquid constant volume again.Be designated as solution C.
Step 4: solution B is added in the solution A, mix, add an amount of acidity regulator, be adjusted to suitable PH, be stirred to dissolving.Add again other physiology cryoprotectant, be stirred to dissolving.Be designated as solution D.
Step 5: solution C is mixed with solution D, add wetting Agent for Printing Inks, then modulation evenly.
The present invention compared with prior art, existing following advantage and effect: 1, take natural organic substance as main raw material, use safety, can not cause tea tree and person poultry poisoning's event, can't cause environmental pollution.2, strengthening the cold resistant while of tea tree, can improve to a certain extent output.3, complete processing is simple, without raw material problem in short supply.4, production cost is low, and product is easy to use.
Description of drawings
Fig. 1 is preparation technology's schema of tea tree cold-resistance agent of the present invention.
Embodiment
The present invention is done the detailed description in a nearly step below in conjunction with drawings and Examples.
This tea tree cold-resistance agent contains bamboo vinegar liquid, physiology cryoprotectant, growth helping matter, acidity regulator etc.Wherein the weight percent of bamboo vinegar liquid is 75-85%, the weight percent of physiology cryoprotectant is 8-14%, the weight percent of growth helping matter is 6-9%, and the weight percent of acidity regulator is 0.5-1%, and the weight percent of other auxiliary agent such as wetting Agent for Printing Inks solubilizing agent etc. is 0.5-1%.Wetting Agent for Printing Inks described in this tea tree cold-resistance agent is animal/vegetable protein glue; Described solubilizing agent is tween-80, dehydrated alcohol.
The physiology cryoprotectant can be for rare earth element, dormin, carbohydrate, Whitfield's ointment, organonitrogen, organic calcium a kind of or whole.Its rare earth elements can be Lanthanum trichloride or Cerium II Chloride; Carbohydrate can be sucrose, glucose, lactose or wood sugar; Organonitrogen can be animal/vegetable protein glue or each seed amino acid, and wherein animal/vegetable protein glue can be gelatine, gelatin, agar or natural resin; Organic calcium can be citrate of lime, calglucon or calcium lactate.
Growth helping matter can be a kind of of Macro and microelements and tethelin or more than one.What wherein Macro and microelements can be for potassium primary phosphate, ammonium molybdate, magnesium chloride, zinc chloride is a kind of or whole; Wherein tethelin can be a kind of of Plant hormones regulators,gibberellins, phytokinin, triacontanol price quote and other plant endogenous hormones or more than one.
Acidity regulator is that calcium hydroxide is or/and potassium hydroxide.
Wherein, animal/vegetable protein glue had not only played the effect of wetting Agent for Printing Inks but also had played the effect of cryoprotectant; Macro and microelements, what have not only plays the effect of growth helping matter but also plays the effect of cryoprotectant.
Embodiment 1:
The tea tree cold-resistance agent of present embodiment is comprised of bamboo vinegar liquid, gelatin, magnesium chloride, zinc chloride, ammonium molybdate, triacontanol price quote, glucose, calglucon, proline(Pro), choline dichloride, calcium hydroxide, tween-80, dehydrated alcohol, wherein bamboo vinegar liquid 80%, gelatin 1%, magnesium chloride 6%, zinc chloride 3%, ammonium molybdate 0.1%, triacontanol price quote 0.05%, glucose 2.4%, calglucon 3.2%, proline 3 .12%, choline dichloride 1%, calcium hydroxide 0.1%, tween-80 0.01%, dehydrated alcohol 0.02%.
Embodiment 2:
The tea tree cold-resistance agent of present embodiment is comprised of bamboo vinegar liquid, gelatin, magnesium chloride, zinc chloride, ammonium molybdate, triacontanol price quote, glucose, dormin, calcium chloride, proline(Pro), choline dichloride, calcium hydroxide, tween-80, dehydrated alcohol, wherein bamboo vinegar liquid 80%, gelatin 1%, magnesium chloride 6%, zinc chloride 3%, ammonium molybdate 0.1%, triacontanol price quote 0.05%, glucose 2.31%, dormin 0.51%, calcium chloride 3.2%, proline(Pro) 2.8%, choline dichloride 1%, calcium hydroxide 0.1%, tween-80 0.01%, dehydrated alcohol 0.02%.
Embodiment 3:
The tea tree cold-resistance agent of present embodiment is by bamboo vinegar liquid, gelatin, potassium primary phosphate, magnesium chloride, zinc chloride, ammonium molybdate, triacontanol price quote, sucrose, calglucon, proline(Pro), Whitfield's ointment, choline dichloride, calcium hydroxide, tween-80, dehydrated alcohol forms, and wherein bamboo vinegar liquid 78.5%, gelatin 1%, potassium primary phosphate 1.5%, magnesium chloride 6%, zinc chloride 3%, ammonium molybdate 0.1%, triacontanol price quote 0.05%, sucrose 1%, calglucon 3.2%, proline(Pro) 2.8%, Whitfield's ointment 0.32%, choline dichloride 2.4%, calcium hydroxide 0.1%, tween-80 0.01%, dehydrated alcohol 0.02%.
Embodiment 4:
The tea tree cold-resistance agent of present embodiment is comprised of bamboo vinegar liquid, gelatin, Lanthanum trichloride, magnesium chloride, zinc chloride, ammonium molybdate, triacontanol price quote, glucose, calcium lactate, halfcystine, choline dichloride, calcium hydroxide, tween-80, dehydrated alcohol, wherein bamboo vinegar liquid 78.5%, gelatin 1%, Lanthanum trichloride 1.5%, magnesium chloride 6%, zinc chloride 3%, ammonium molybdate 0.1%, triacontanol price quote 0.05%, glucose 1%, calcium lactate 3.2%, halfcystine 3.12%, choline dichloride 2.4%, calcium hydroxide 0.1%, tween-80 0.01%, dehydrated alcohol 0.02%.
Embodiment 5:
The tea tree cold-resistance agent of present embodiment is comprised of bamboo vinegar liquid, gelatin, magnesium chloride, zinc chloride, ammonium molybdate, triacontanol price quote, sucrose, calcium lactate, halfcystine, Whitfield's ointment, calcium hydroxide, tween-80, dehydrated alcohol, wherein bamboo vinegar liquid 81.4%, gelatin 1%, magnesium chloride 6%, zinc chloride 3%, ammonium molybdate 0.1%, triacontanol price quote 0.05%, sucrose 2%, calcium lactate 3.2%, Cys2 .8%, Whitfield's ointment 0.32%, calcium hydroxide 0.1%, tween-80 0.01%, dehydrated alcohol 0.02%.
Embodiment 6:
The tea tree cold-resistance agent of present embodiment is comprised of bamboo vinegar liquid, gelatin, potassium primary phosphate, magnesium chloride, zinc chloride, ammonium molybdate, Plant hormones regulators,gibberellins, glucose, calcium chloride, proline(Pro), choline dichloride, potassium hydroxide, tween-80, dehydrated alcohol, wherein bamboo vinegar liquid 80%, gelatin 1%, potassium primary phosphate 1.5%, magnesium chloride 6%, zinc chloride 3%, ammonium molybdate 0.1%, Plant hormones regulators,gibberellins 0.05%, glucose 1%, calcium chloride 3.2%, proline 3 %, choline dichloride 1%, potassium hydroxide 0.12%, tween-80 0.01%, dehydrated alcohol 0.02%.
Preparation method's example:
Referring to Fig. 1, to fill a prescription 2 as example, preparation method of the present invention is divided into following step.
Step 1: in the bamboo vinegar liquid that accounts for prescription total mass 60%, add 6% magnesium chloride, be stirred to dissolving; Add 3% zinc chloride and be stirred to dissolving; Add 0.1% ammonium molybdate, be stirred to dissolving.Be designated as solution A.
Step 2: take by weighing 0.51% dormin, be dissolved in 0.01% dehydrated alcohol.Be designated as solution B.
Step 3: take by weighing 0.05% triacontanol price quote, be dissolved in 0.01%80 ℃ dehydrated alcohol, add 80 ℃ 0.01% tween-80, prescription total mass 20% bamboo vinegar liquid that accounts for that incorporates 1% gelatin that then adds again 80 ℃ is settled to 200ml.Be designated as solution C.
Step 4: solution B is added in the solution A, mix, add 0.1% calcium hydroxide.Regulate about PH to 3.70.Add 3.2% calcium chloride, be stirred to dissolving; Add 2.8% proline(Pro), be stirred to dissolving; Add 1% choline dichloride, be stirred to dissolving.Be designated as solution D.
Step 5: solution C is added in the solution D, mix, add 2.31% glucose, then modulation is even, and last packing gets final product.
Per-cent of the present invention all is weight percentage.
Example:
1, this cold-resistance agent is processed the test-results of potted plant tea tree
Table 1
Figure BSA00000710932800041
From the results shown in Table 1, tea tree cold-resistance agent of the present invention can both significantly improve SOD enzymic activity, soluble protein content, soluble sugar content and the proline content in the tealeaves before and after subzero treatment, thereby improves the cold tolerance of tea tree.And the mda content after this tea tree cold-resistance agent is processed is less than control group, and explanation can alleviate cold damage to a certain extent to the infringement of tealeaves.
2, the field experiment result of this cold-resistance agent.
Spray the time: on March 18th, 2011 first time; On March 23rd, 2011 second time; On March 28th, 1.
Test site: the tea plantation of Yuhang Xi Ansi village Ceng Qingshu
Tea tree breed: meet frost
The examination time: on April 8th, 2011
Spray processing: the clear water contrast; Other cold-resistance agents; Cold-resistance agent of the present invention
Table 2
Figure BSA00000710932800051
Annotate: 0.1m 2Bright (doing) heavy=hundred buds bright (doing) weight * 0.1m 2Bud head number/100
From the results shown in Table 2, tea tree cold-resistance agent of the present invention can significantly improve the thickness that bud density, first intact leaves of tealeaves, thereby improves the cold tolerance of tea tree.Simultaneously, tea tree cold-resistance agent of the present invention also can improve the tea shoot weight in the chlorophyll content of tea tree, the reguarity that sprouts (ratio of two leaves and a bud is the highest, reaches 73%) and the unit surface, has effectively increased the output of tealeaves.

Claims (13)

1. a tea tree cold-resistance agent is characterized in that: contain bamboo vinegar liquid, physiology cryoprotectant, growth helping matter, acidity regulator etc.
2. tea tree cold-resistance agent according to claim 1, it is characterized in that: wherein the weight percent of bamboo vinegar liquid is 75-85%, the weight percent of physiology cryoprotectant is 8-14%, the weight percent of growth helping matter is 6-9%, the weight percent of acidity regulator is 0.5-1%, and the weight percent of other auxiliary agent is 0.5-1%.
3. tea tree cold-resistance agent according to claim 1, it is characterized in that: can also add wetting Agent for Printing Inks, described wetting Agent for Printing Inks is animal/vegetable protein glue.
4. tea tree cold-resistance agent according to claim 1, it is characterized in that: can also add solubilizing agent, described solubilizing agent is tween-80, dehydrated alcohol.
5. tea tree cold-resistance agent according to claim 1 is characterized in that: described physiology cryoprotectant can be a kind of of rare earth element, dormin, carbohydrate, Whitfield's ointment, organonitrogen, organic calcium or more than one; Described growth helping matter can be a kind of of Macro and microelements and tethelin or more than one; Described acidity regulator is that calcium hydroxide is or/and potassium hydroxide.
6. tea tree cold-resistance agent according to claim 5, it is characterized in that: described rare earth element can be Lanthanum trichloride or Cerium II Chloride.
7. tea tree cold-resistance agent according to claim 5, it is characterized in that: described carbohydrate can be sucrose, glucose, lactose or wood sugar.
8. according to claim 3 or 5 described tea tree cold-resistance agents, it is characterized in that: described organonitrogen can be animal/vegetable protein glue or each seed amino acid, and wherein animal/vegetable protein glue can be gelatine, gelatin, agar or natural resin.
9. tea tree cold-resistance agent according to claim 5, it is characterized in that: described organic calcium can be citrate of lime, calglucon or calcium lactate.
10. tea tree cold-resistance agent according to claim 5 is characterized in that: what described Macro and microelements can be for potassium primary phosphate, ammonium molybdate, magnesium chloride, zinc chloride is a kind of or whole.
11. tea tree cold-resistance agent according to claim 5 is characterized in that: described tethelin can be a kind of of Plant hormones regulators,gibberellins, phytokinin, triacontanol price quote and other plant endogenous hormones or more than one.
12. the preparation method of a tea tree cold-resistance agent is characterized in that: be divided into following step.
Step 1: get in proportion required bamboo vinegar liquid, the Macro and microelements that adding need be joined is stirred to dissolving.Be designated as solution A.
Step 2: the physiology cryoprotectant that need join is dissolved in the dehydrated alcohol.Be designated as solution B.
Step 3: the tethelin that need join is dissolved in the dehydrated alcohol of heat, uses the bamboo vinegar liquid constant volume again.Be designated as solution C.
Step 4: solution B is added in the solution A, mix, add an amount of acidity regulator, be adjusted to suitable PH, be stirred to dissolving.Add again other physiology cryoprotectant, be stirred to dissolving.Be designated as solution D.
Step 5: solution C is mixed with solution D, and then modulation evenly.
13. tea tree cold-resistance agent according to claim 1 is characterized in that: all right mixed solubilizers, wetting Agent for Printing Inks etc. in the described bamboo vinegar liquid, and through being stirred to fully dissolving.
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Application publication date: 20130123