CN103238545B - Device and method for hatching fertilized eggs of sepia esculenta - Google Patents

Device and method for hatching fertilized eggs of sepia esculenta Download PDF

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Publication number
CN103238545B
CN103238545B CN201310194308.XA CN201310194308A CN103238545B CN 103238545 B CN103238545 B CN 103238545B CN 201310194308 A CN201310194308 A CN 201310194308A CN 103238545 B CN103238545 B CN 103238545B
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connecting rod
hatching
nido
diameter
basket
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CN103238545A (en
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刘长琳
陈四清
赵法箴
刘春胜
薛祝家
孙建明
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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Abstract

A device for hatching fertilized eggs of sepia esculenta comprises a nested hatching basket and a support rod. The nested hatching basket is provided with a bottom, the bottom is similar to a honeycomb and is provided with a central hole, the bottom similar to the honeycomb comprises a series of barrels, a circular hole is arranged at the bottom of each barrel, and the diameter of each circular hole is smaller than that of the corresponding barrel. By the aid of the device, fertilized eggs of the sepia esculenta are independently hatched in the nested hatching basket. As the diameter of each fertilized egg is larger than that of each barrel, the fertilized eggs cannot be clamped into the barrels, and later expansion of the fertilized eggs cannot be affected. Besides, by the aid of the circular holes at the barrel-shaped bottom, circulation and exchange of water can be ensured when the fertilized eggs are hatched, oxygen deficiency caused by squeezing of the fertilized eggs can be avoided, and saprolegniasis is effectively avoided. Hatched young sepia esculenta can immediately swim out by themselves from the side wall of the hatching basket, all egg shells fall into the barrels at the bottom of the hatching basket, the egg shells and fries are easily separated, the water in a nursery pond is kept clean, stimulation damage is effectively decreased in the fry cultivation process, and the hatching rate of the fertilized eggs is finally increased.

Description

A kind of golden cuttlefish incubating oosperm device and hatching m thereof
Technical field
The invention belongs to aquaculture field, relate to particularly a kind of golden cuttlefish incubating oosperm device and hatching m thereof.
Background technology
Golden cuttlefish is cultivation new varieties newly developed, the feature such as there is life cycle short (1 year), fast growth, individuality is large, nutritious, availability is high, migration rule is obvious, and aquaculture has a extensive future.At present, the cultivation scale of golden cuttlefish and scope just progressively expand.
The ovum of the firm output of golden cuttlefish, counterpoise 0.99g/ grain (n=100), major axis is 11~24mm, minor axis is 8~15mm, outer by tertiary egg envelope.Tertiary egg envelope experiences the change procedure of a softness-formalize-dwindle-expand in embryo development procedure.Fertilized egg is hatched under 20~25 DEG C of water temperatures, through 25~30 days young successively demoulding hatch.Before membrane, fertilized egg egg membrane becomes very thin, sparkling and crystal-clear bright, 2 times of left and right when volume is firm output.After young cuttlefish hatches, when chorion is punctured into firm output 1/4~1/3 of volume.
Golden cuttlefish has the ovum of extension characteristic, generally in parents culture pond, places the article such as sea cucumber basket frame, net sheet or sieve basin as spawning base, adheres to for hanging ovum.Before the present invention makes, the hatching pattern of golden cuttlefish fertilized egg is mainly adherance to be directly transferred to hatchery hatching from parents culture pond, due to golden cuttlefish brooding time long (20~25 DEG C need 25~30 days), rot to hatching the as easy as rolling off a log large area that causes in ovum amount concentrated area on later stage adherance, and then have a strong impact on incubation rate.In addition, due in the hatching later stage, fertilized egg is expanded rear egg membrane and is become very thin, mobile or as easy as rolling off a logly while shifting adherance cause seed to hatch in advance because of stimulation, and then affect hatching quality and early stage survival rate, therefore on producing, generally after seed all hatches, the adherance that hangs with chorion is shifted out, seed rearing pond is changed in incubating oosperm pond into.But, there are a large amount of chorions to come off from adherance due to after hatching successively in seed, the chorion coming off and golden cuttlefish seed are mixed in together, as easy as rolling off a log putrid and deteriorated and breed bacteria, in addition due to the life of dwelling at the end of golden cuttlefish seed stage battalion, most of time transfixion easily causes seed ink-jet damage, and then affects the early stage survival rate of seed rearing in the time removing chorion.Have no at present the report of the special hatching apparatus of golden cuttlefish fertilized egg and hatching m thereof.
Summary of the invention
The technical problem to be solved in the present invention is according to golden cuttlefish development of fertilized ova characteristic, and a kind of special hatching apparatus and hatching m thereof are provided, and hatches rear seed and extremely difficult this technical barrier that separates of chorion to improve golden cuttlefish hatching rate and to solve seed.
The present invention completes by following operating technology:
A kind of golden cuttlefish incubating oosperm device, comprises nido hatching basket and support bar, nido hatching basket has similar honeycomb bottom and bottom has a medium pore, and the drum that similar honeycomb bottom has a circular hole by a series of bottoms forms, and the diameter of circular hole is less than drum diameter, and nido hatching basket sidewall is sieve-mesh, support bar is made up of 1 pedestal and more than one sleeving connecting rod, pedestal comprises that one has the base of constant weight and is vertically connected to the connecting rod on base, sleeving connecting rod divides two sections, one section is thin segment, another section is thick section, between thin segment and thick section, it is rectangular transition, the diameter of connecting rod is identical with thin segment diameter, in thick section of connecting rod insertion sleeving connecting rod, the medium pore diameter of nido hatching basket is less than thick section of diameter of sleeving connecting rod, and be greater than the thin segment diameter of sleeving connecting rod, hatch the medium pore of basket through nido by the thin segment of sleeving connecting rod, thereby nido hatching basket is fixed on to the thin segment and thick section junction of sleeving connecting rod, thick section of inserting another sleeving connecting rod by the thin segment of a sleeving connecting rod like this, then by nido hatch basket medium pore be inserted in sleeving connecting rod thin segment, form the golden cuttlefish incubating oosperm device of multilayer nido hatching basket one.
Further, the described high 10~12cm of nido hatching basket, base diameter 36~40cm; Sidewall sieve diameter 7~8mm, is convenient to just to incubate the young and swims out of from sidewall, the drum diameter 6~8mm of similar honeycomb bottom, and the diameter 2~3mm of drum bottom circular aperture, in case chorion exposes.
The present invention also provides a kind of method of utilizing foregoing invention device to carry out golden cuttlefish incubating oosperm, pedestal is put into hatchery, put a sleeving connecting rod, on sleeving connecting rod, penetrate a nido hatching basket, according to actual needs with the hatchery depth of water, then cover connects sleeving connecting rod and the nido hatching basket of requirement; Fertilized egg is taken from adherance, put into the nido hatching basket hatching of this hatching apparatus, on the drum of a similar honeycomb bottom, place a fertilized egg, after incubation of membrane, just incubating the young swims out of from nido hatching basket sidewall, chorion is fallen in the drum of similar honeycomb bottom, after incubating oosperm finishes, shifts out apparatus of the present invention.
The beneficial effect that the present invention is compared with the prior art:
1) fertilized egg is taken from adherance, putting into this hatching apparatus hatches, after young cuttlefish hatches, can swim out of from hatching basket sidewall voluntarily at once, chorion all falls in the drum of hatching basket bottom, be easy to chorion and seed to separate, thereby in maintenance nursery pond, water quality is clean, effectively reduce the stimulation damage in seed rearing process, significantly improved seed rearing survival rate.
2) apparatus of the present invention make the hatching separately in nido hatching basket of golden cuttlefish fertilized egg, because fertilized egg diameter is greater than drum diameter, therefore fertilized egg can not snap in drum inside, do not affect late expansion, drum type bottom has circular hole simultaneously, and can ensure incubating oosperm time, the circulation of water quality exchange, can avoid the anoxic causing because of fertilized egg extruding, and then effectively avoid the generation of saprolegniasis, finally improve the incubation rate of fertilized egg.
3) hatching apparatus is divided into multilayer, can increase and decrease with the hatchery depth of water according to actual needs.
Brief description of the drawings
Fig. 1 nido hatching basket front view: 4, nido hatching basket sidewall;
The similar honeycomb of Fig. 2 bottom: 1, medium pore, 2, circular hole;
Fig. 3 a, drum front view: 2, circular hole; 3, drum; B, golden cuttlefish fertilized egg are placed on the state on drum: 10, fertilized egg;
Fig. 4 nido hatching basket upward view: 2, circular hole;
Fig. 5 a, sleeving connecting rod; B, pedestal: 5, base, 6, connecting rod; The assembled state of c pedestal and sleeving connecting rod: 7 thin segments, 8 thick sections, 9 junctions.
Embodiment
Describe technical method of the present invention in detail below by embodiment:
Embodiment 1
A kind of golden cuttlefish incubating oosperm device, comprises nido hatching basket (as shown in Figure 1) and support bar, nido hatching basket has similar honeycomb bottom (as shown in Figure 2), and bottom has a medium pore 1, the drum 3 that similar honeycomb bottom (as shown in Figure 2) has a circular hole 2 by a series of bottoms forms, as shown in Figure 3 a, the diameter of circular hole 2 is less than drum 3 diameters, and nido hatching basket sidewall 4 is sieve-meshs, the upward view of nido hatching basket as shown in Figure 4, support bar is made up of 1 pedestal (as shown in Figure 5 b) and more than one sleeving connecting rod (as shown in Figure 5 a), pedestal comprises that one has the base 5 of constant weight and is vertically connected to the connecting rod 6 on base, sleeving connecting rod divides two sections, one section is thin segment 7, another section is thick section 8, between thin segment and thick section, it is rectangular transition (as shown in Figure 5 a), the diameter of connecting rod 6 is identical with thin segment 7 diameters, connecting rod 6 inserts in thick section 8 of sleeving connecting rod, medium pore 1 diameter of nido hatching basket is less than thick section of 8 diameters of sleeving connecting rod, and be greater than thin segment 7 diameters of sleeving connecting rod, the thin segment of sleeving connecting rod is through the medium pore of nido hatching basket, thereby nido hatching basket is fixed on to the thin segment and thick section junction 9 of sleeving connecting rod, thick section of inserting another sleeving connecting rod by the thin segment of a sleeving connecting rod like this, then the medium pore of nido hatching basket is inserted in connecting rod thin segment, form the golden cuttlefish incubating oosperm device of multilayer nido hatching basket one.
The described high 10cm of nido hatching basket, can be also the arbitrary specification of 10-12cm, and base diameter 36cm, can be also the arbitrary specification of 36-40cm; Sidewall sieve diameter 7mm, can be also the arbitrary specification of 7-8cm, is convenient to just to incubate the young and swims out of from sidewall; The drum diameter 6mm of similar honeycomb bottom, can be also the arbitrary specification of 6-8cm, and the diameter 2mm of drum bottom circular aperture, can be also the arbitrary specification of 2-3cm, in case chorion exposes.
The present invention also provides a kind of method of utilizing foregoing invention device to carry out golden cuttlefish incubating oosperm, pedestal is put into hatchery, put a sleeving connecting rod, as shown in Figure 5 c, on sleeving connecting rod, penetrate a nido hatching basket, according to actual needs with the hatchery depth of water, then cover connects sleeving connecting rod and the nido hatching basket of requirement; Fertilized egg is taken from adherance, put into the nido hatching basket hatching of this hatching apparatus, on the drum of a similar honeycomb bottom, place a fertilized egg 10, as shown in Figure 3 b, after incubation of membrane, just to incubate the young and swim out of from nido hatching basket sidewall, chorion is fallen in the drum of similar honeycomb bottom, after incubating oosperm finishes, shift out apparatus of the present invention.
In June, 2012, we have carried out the effect experiment of the different hatching ms of golden cuttlefish fertilized egg in Golden Beach, Qingdao aquatic products development corporation, Ltd..Incubation condition is: 20~25 DEG C of water temperatures, and pH7.8~8.2, salinity 28~35, dissolved oxygen amount is greater than 6mg/L, uninterrupted inflation continuously, a day rate of water exchange is 200%~400%, intensity of illumination 500Lx~600Lx.
1) first method adopts apparatus of the present invention and method, and pedestal is put into hatchery, puts a sleeving connecting rod, penetrates a nido hatching basket on sleeving connecting rod, and according to actual needs with the hatchery depth of water, then cover connects 6 sleeving connecting rods and nido hatching basket; Fertilized egg is taken from adherance, put into the hatching of nido hatching basket, hatching depth of water 90cm, each hatching apparatus has 6 layers of hatching basket, altogether approximately 1200 fertilized egg.Result demonstration, fertilized egg is not caused extruding in hatching process, and water body exchange is good, and water mold disease does not occur, and incubation rate is 99%.After young cuttlefish hatches, can swim out of from hatching basket sidewall voluntarily at once, chorion all falls in the drum of hatching basket bottom, thereby has effectively reduced the stimulation damage in seed rearing process, and seed rearing survival rate is promoted to 60~70% by 20~30%.
2) second method, by adherance from shift out in parents culture pond, directly put into hatchery hatching, result shows, the place in adherance ovum quantity set, and the pathogenetic ratio of water mold is up to 72%, incubation rate is only 65%, the chorion ratio of coming off accounts for more than 30%, the golden cuttlefish young is caused and stress ink-jet be damaged when causing bacteria breed and remove chorion due to chorion corruption in addition, and seed rearing survival rate is only 20~30%.
3) the third method, takes fertilized egg from adherance, puts into common plastic screen basin and hatches.Result shows, hatchability of fertile eggs is 85%, may not be independent arrangement with fertilized egg, the as easy as rolling off a log extruding anoxic that causes be relevant, in addition because sieve basin aperture is less, young cuttlefish hatches and is difficult to proceed to voluntarily seed rearing pond, due to seed membrane Time Inconsistency, cause the young early hatching cannot feed into bait, thereby have influence on normal initial feeding, if pull out with dab net, as easy as rolling off a logly cause ink-jet damage and affect survival rate.Adopt the method, seed rearing survival rate is only 30~40%.In addition while adopting the method hatching, because sieve basin is individual layer, in a nursery pond, hatch fertilized egg limited amount, while carrying out scale hatching, need multiple hatcheries, thereby cause cost to increase and water resource waste.

Claims (3)

1. a golden cuttlefish incubating oosperm device, is characterized in that it comprises nido hatching basket and support bar, nido hatching basket has similar honeycomb bottom and bottom has a medium pore (1), the drum (3) that similar honeycomb bottom has a circular hole (2) by a series of bottoms forms, the diameter of circular hole (2) is less than drum (3) diameter, and nido hatching basket sidewall (4) is sieve-mesh, support bar is made up of 1 pedestal and more than one sleeving connecting rod, pedestal comprises that one has the base (5) of constant weight and is vertically connected to the connecting rod (6) on base, sleeving connecting rod divides two sections, one section is thin segment (7), another section is thick section (8), between thin segment and thick section, it is rectangular transition, the diameter of connecting rod is identical with thin segment diameter, connecting rod inserts in thick section (8) of sleeving connecting rod, medium pore (1) diameter of nido hatching basket is less than thick section of (8) diameter of sleeving connecting rod, and be greater than thin segment (7) diameter of sleeving connecting rod, hatch the medium pore of basket through nido by the thin segment of sleeving connecting rod, thereby nido hatching basket is fixed on to the thin segment and thick section junction (9) of sleeving connecting rod, thick section (8) of so inserting another sleeving connecting rod by the thin segment (7) of a sleeving connecting rod, then by nido hatch basket medium pore (1) be inserted in sleeving connecting rod thin segment, form the golden cuttlefish incubating oosperm device of multilayer nido hatching basket one.
2. a kind of golden cuttlefish incubating oosperm device according to claim 1, is characterized in that the described high 10~12cm of nido hatching basket, base diameter 36~40cm; Sidewall sieve diameter 7~8mm, is convenient to just to incubate the young and swims out of from sidewall, the drum diameter 6~8mm of similar honeycomb bottom, and the diameter 2~3mm of drum bottom circular aperture, in case chorion exposes.
3. a method of utilizing one of foregoing invention device to carry out golden cuttlefish incubating oosperm, pedestal is put into hatchery, put a sleeving connecting rod, on sleeving connecting rod, penetrate a nido hatching basket, according to actual needs with the hatchery depth of water, then cover connects sleeving connecting rod and the nido hatching basket of requirement; Fertilized egg is taken from adherance, put into the nido hatching basket hatching of this hatching apparatus, on the drum of a similar honeycomb bottom, place a fertilized egg (10), after incubation of membrane, just incubating the young swims out of from nido hatching basket sidewall, chorion is fallen in the drum of similar honeycomb bottom, after incubating oosperm finishes, shifts out apparatus of the present invention.
CN201310194308.XA 2013-05-23 2013-05-23 Device and method for hatching fertilized eggs of sepia esculenta Expired - Fee Related CN103238545B (en)

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Publication number Priority date Publication date Assignee Title
TWI650071B (en) * 2018-01-22 2019-02-11 國立臺灣海洋大學 Incubation system and method thereof for aquatic creatures
CN108432666B (en) * 2018-03-22 2020-10-27 中国水产科学研究院黄海水产研究所 Long-distance transportation method for fertilized eggs of Sepiella maindroni
CN109496928A (en) * 2018-11-27 2019-03-22 浙江海洋大学 A kind of full-automatic hatching larvae device and method suitable for bottom dwelling property cuttlefish

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