CN103232978A - Preparation method of plant SOD (superoxide dismutase) for treating burns and scalds through external application - Google Patents
Preparation method of plant SOD (superoxide dismutase) for treating burns and scalds through external application Download PDFInfo
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- CN103232978A CN103232978A CN2013101521891A CN201310152189A CN103232978A CN 103232978 A CN103232978 A CN 103232978A CN 2013101521891 A CN2013101521891 A CN 2013101521891A CN 201310152189 A CN201310152189 A CN 201310152189A CN 103232978 A CN103232978 A CN 103232978A
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Abstract
The invention relates to a preparation method of plant SOD (superoxide dismutase) for treating burns and scalds through external application, which comprises the following steps: cleaning Silphium perfoliatum, pulping, juicing, and heating at 60-65 DEG C; cooling the heated liquid to room temperature, and performing precipitation; adding edible lactic acid, and performing secondary precipitation; pouring a stock solution into a barrel, and filtering to separate out a transparent clear water solution, thus obtaining a water soluble protein; performing secondary filtration to separate the water and the SOD protein, and sterilizing the obtained liquid solvent; and finally, filling to obtain a finished product. The invention has the following advantages: the method is used for extracting the SOD protein, and the method is simple; the SOD product contains no chemical component; the time for treating patients with burns and scalds is shortened; after burns and scalds are cured, the patients have no scar left, thus protecting the skin of the patients with burns and scalds; and meanwhile, the pain of the patients is reduced.
Description
Technical field
The present invention relates to the field of Chinese medicines, relate in particular to a kind of plant SOD(superoxide-dismutase of external application for curing burn and scald) the preparation method.
Background technology
The cause of disease of burn and scald mainly contains: fire, boiling water, deep fat, chemical substance, metallic solution etc., since the heat-flash effect, the infringement human body, the lighter's skin and flesh rots, and weight person injures internal organs.Doctor trained in Western medicine mainly is to adopt the antibiotics infusion treatment clinically, serious takes skin to move to grow operation, and patient is very painful, and skin leaves scar, and burden economically is very heavy.At present, the prescription for the treatment of burn and scald is a lot, but it has many shortcomings: (1) can only external application, can not be oral, and contain the Chemical Composition of wound body; (2) respond well to treatment slowly, treatment burn and scald patient's time is long; (3) during the patient, agonizing, residual scar also.
Summary of the invention
The plant SOD(superoxide-dismutase that the purpose of this invention is to provide a kind of external application for curing burn and scald) preparation method, the deficiency that exists with the existing method that overcomes burn and scald.
The objective of the invention is to be achieved through the following technical solutions:
A kind of plant SOD(superoxide-dismutase of external application for curing burn and scald) preparation method, its raw material adopts pure natural plants string leaf rosinweed, and described this method may further comprise the steps:
(1) will go here and there after the leaf rosinweed cleans, and pull an oar, squeeze the juice, and use heating tank to heat under 60-65 degree centigrade temperature, described heating tank is double-deck HA type thermostatically heating pot;
(2) liquid after will heating carries out deepfreeze, is cooled to room temperature, carries out static state deposition then, and sedimentation time is 8-10 hour;
(3) post precipitation adds edible lactic acid, and every 100kg adds the edible lactic acid of 20 grams, carries out secondary sedimentation, and sedimentation time is 10 hours;
(4) after the secondary sedimentation, stoste is poured in the bucket, added 100kg silicon bentonite, and adopt filter to filter, described filter is ZJL03 or 02 type filter; And
(5) after the filtration, separation just goes out transparent clear water liquid, carries out precipitation then three times, and obtain water-soluble protein this moment, carries out essence then and filters, and water is separated with SOD protein, and the liquid solvent that draws carries out sterilization, at last can SOD protein.
Further, the SOD supernatant liquor of above method preparation is for the preparation of the SOD oral liquid for the treatment of burn and scald; The SOD protein of above method preparation is for the preparation of the SOD emulsion for the treatment of burn and scald.
Further, the SOD emulsion of described treatment burn and scald is mixed by the SOD protein of 20% distilled water and 80% and forms, and above per-cent is mass percent.
Further, the SOD oral liquid of described treatment burn and scald is made up of 40% distilled water and 60% SOD supernatant liquor, and above per-cent is mass percent.
Beneficial effect of the present invention is: present method is used for extracting SOD albumen, and method is simple, and the SOD product does not contain chemical ingredients; shortened the time of burn and scald patient; cure the back patient and can not stay scar, protected burn and scald patient's skin, reduced patient's misery simultaneously.
Embodiment
The preparation method of the plant SOD of the described a kind of external application for curing burn and scald of the embodiment of the invention, its raw material adopts pure natural plants string leaf rosinweed, and described this method may further comprise the steps:
(1) will go here and there after the leaf rosinweed cleans, and pull an oar, squeeze the juice, and use heating tank to heat under 60-65 degree centigrade temperature, described heating tank is double-deck HA type thermostatically heating pot;
(2) liquid after will heating carries out deepfreeze, is cooled to room temperature, carries out static state deposition then, and sedimentation time is 8-10 hour;
(3) post precipitation adds edible lactic acid, and every 100kg adds the edible lactic acid of 20 grams, carries out secondary sedimentation, and sedimentation time is 10 hours;
(4) after the secondary sedimentation, stoste is poured in the bucket, added 100kg silicon bentonite, and adopt filter to filter, described filter is ZJL03 or 02 type filter; And
(5) after the filtration, separation just goes out transparent clear water liquid, carries out precipitation then three times, and obtain water-soluble protein this moment, carries out essence then and filters, and water is separated with SOD protein, and the liquid solvent that draws carries out sterilization, at last the can finished product.
Adopt pollution-free green plant rhizome leaf, through sterilization, sterilization, extract supernatant liquor with adding edible lactic acid method, then supernatant liquor is refined into the medicine of two kinds for the treatment of burn and scalds, the SOD supernatant liquor of preparation is for the preparation of the SOD oral liquid for the treatment of burn and scald; The SOD protein of preparation is for the preparation of the SOD emulsion for the treatment of burn and scald.The SOD emulsion of described treatment burn and scald is mixed by the SOD protein of 20% distilled water and 80% and forms; The SOD oral liquid of described treatment burn and scald is made up of 40% distilled water and 60% SOD supernatant liquor, and above per-cent is mass percent.
The example of curing nearly 100 burn and scald patients from nearly 5 years proves that effect is remarkable, treats efficient reaching more than 95%.The easy cryopreservation of the present invention in addition, it is standing to be convenient to family, if any burn and scald, can avoid running hospital's delay treating time of registering, line up, and need not to be in hospital patient's following specific embodiment of can getting timely medical treatment:
Example 1 Mrs Gao, 30 years old, once the shank burn surface area was serious, gnawing pains take no rest, repeatedly makes to heal with medicine, and can't ease the pain at that time, with SOD emulsion of the present invention external application, after the use, pain relief, and after using several days continuously, pain stops, the burn site recovery from illness, expense is lower, reduces by three fens more than two than other drug medical expense.
Example 2 Mr.s Sun, 40 years old, foot's burn, wound is serious, repeatedly makes to heal with medicine, and use antibiotic medicine, general take 20-30 days and just can cure, use SOD emulsion of the present invention external application after, only need just can heal a wound in 7-10 days fully, expense is lower, reduces by three fens more than two than other drug medical expense.
Example 3 Mr. Lius, 35 years old, shank was injured, used the antibiotics injection for curing, and play the garter anti-inflammatory of infusing, treatment time is long, and is agonizing, uses SOD emulsion of the present invention external application, need not beat garter, palliate the agonizing sufferings, expense is lower, reduces by three fens more than two than other drug medical expense.
Example 4 Mrs Yang face minor burns, repeatedly make and heal with medicine, effect is not remarkable, and cure the back wound and stay obvious scar, it is attractive in appearance to influence face, use SOD emulsion of the present invention to cure after, the burn position of 80-90% does not have obvious residual scar, expense is lower, reduces by three fens more than two than other drug medical expense.
The present invention is not limited to above-mentioned preferred forms; anyone can draw other various forms of products under enlightenment of the present invention; no matter but do any variation in its shape or structure; every have identical with a application or akin technical scheme, all drops within protection scope of the present invention.
Claims (7)
1. the preparation method of the plant SOD of an external application for curing burn and scald is characterized in that, said method comprising the steps of:
(1) will go here and there after the leaf rosinweed cleans, and pull an oar, squeeze the juice, and under 60-65 degree centigrade temperature, use heating tank to heat;
(2) liquid after will heating carries out deepfreeze, is cooled to room temperature, carries out static state deposition then, and sedimentation time is 8-10 hour;
(3) post precipitation adds edible lactic acid, and every 100kg adds the edible lactic acid of 20 grams, carries out secondary sedimentation, and sedimentation time is 10 hours;
(4) after the secondary sedimentation, stoste is poured in the bucket, added 100kg silicon bentonite, and adopt filter to filter; And
(5) after the filtration, isolate transparent SOD supernatant liquor, carry out precipitation then three times, obtain water-soluble protein this moment, carries out essence then and filter, and water is separated with SOD protein, and the liquid solvent that obtains carries out sterilization, last can SOD protein.
2. the preparation method of the plant SOD of external application for curing burn and scald according to claim 1, it is characterized in that: in the step (1), described heating tank is double-deck HA type thermostatically heating pot.
3. the preparation method of the plant SOD of external application for curing burn and scald according to claim 2, it is characterized in that: in the step (4), described filter is ZJL03 type or 02 type filter.
4. the SOD supernatant liquor of the preparation method of the plant SOD of the described external application for curing burn and scald of claim 1 preparation is for the preparation of the SOD oral liquid for the treatment of burn and scald.
5. the SOD protein of the preparation method of the plant SOD of the described external application for curing burn and scald of claim 1 preparation is for the preparation of the SOD emulsion for the treatment of burn and scald.
6. the SOD emulsion for the preparation of the treatment burn and scald is characterized in that, described emulsion is mixed by the SOD protein of 20% distilled water and 80% and forms, and above per-cent is mass percent.
7. SOD oral liquid for the preparation of the treatment burn and scald, it is characterized in that: described oral liquid is made up of 40% distilled water and 60% SOD supernatant liquor, and above per-cent is mass percent.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013101521891A CN103232978A (en) | 2013-04-28 | 2013-04-28 | Preparation method of plant SOD (superoxide dismutase) for treating burns and scalds through external application |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013101521891A CN103232978A (en) | 2013-04-28 | 2013-04-28 | Preparation method of plant SOD (superoxide dismutase) for treating burns and scalds through external application |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101590222A (en) * | 2008-05-26 | 2009-12-02 | 刘安荣 | A kind of SOD cell repair liquid and preparation method thereof |
| CN102090500A (en) * | 2010-06-10 | 2011-06-15 | 长沙富仁投资管理有限公司 | Extraction method of medicinal and edible plant leaf protein and SOD (Super Oxide Dlsmutase) |
| CN102010852B (en) * | 2010-10-21 | 2013-01-09 | 湛江师范学院 | Method for extracting sisal hemp superoxide dismutase (SOD) |
| CN102220296B (en) * | 2011-06-02 | 2013-01-30 | 济宁益佳科技有限公司 | Method for extracting garlic superoxide dismutase from garlic sheet processing waste water |
-
2013
- 2013-04-28 CN CN2013101521891A patent/CN103232978A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101590222A (en) * | 2008-05-26 | 2009-12-02 | 刘安荣 | A kind of SOD cell repair liquid and preparation method thereof |
| CN102090500A (en) * | 2010-06-10 | 2011-06-15 | 长沙富仁投资管理有限公司 | Extraction method of medicinal and edible plant leaf protein and SOD (Super Oxide Dlsmutase) |
| CN102010852B (en) * | 2010-10-21 | 2013-01-09 | 湛江师范学院 | Method for extracting sisal hemp superoxide dismutase (SOD) |
| CN102220296B (en) * | 2011-06-02 | 2013-01-30 | 济宁益佳科技有限公司 | Method for extracting garlic superoxide dismutase from garlic sheet processing waste water |
Non-Patent Citations (3)
| Title |
|---|
| 李海等: "超氧化物歧化酶的研究现状", 《塔里木农垦大学学报》 * |
| 王中凤等: "仙人掌SOD提取条件及提取过程活性稳定性研究", 《食品与发酵工业》 * |
| 王雪峰等: "大蒜超氧化物歧化酶的分离纯化及其性质的研究", 《安徽农业科学》 * |
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Application publication date: 20130807 |