CN103232553B - A kind of semi continuous extracts the apparatus and method of polyporusum bellatus - Google Patents
A kind of semi continuous extracts the apparatus and method of polyporusum bellatus Download PDFInfo
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- CN103232553B CN103232553B CN201310171854.1A CN201310171854A CN103232553B CN 103232553 B CN103232553 B CN 103232553B CN 201310171854 A CN201310171854 A CN 201310171854A CN 103232553 B CN103232553 B CN 103232553B
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Abstract
The invention discloses the apparatus and method that a kind of semi continuous extracts polyporusum bellatus, comprise extraction agent storage tank (1), extraction agent vacuum primingpump (2), ultrasonic-assisted extraction device (5), force (forcing) pump (6), hollow membrane tripping device (9), concentrated solution collector (10); Clear liquid collector (11); The molecular weight cut-off of hollow membrane tripping device (9) is 10000; Extraction agent sucks and joins in ultrasonic-assisted extraction device (5) by extraction agent vacuum primingpump (2), ultrasonic-assisted extraction device (5) outlet is connected with hollow membrane tripping device (9) by force (forcing) pump (6), concentrated solution enters concentrated solution collector (10), next step alcohol pending is analysed, is precipitated, and clear liquid enters clear liquid collector (11).The advantages such as the present invention take umbellate pore furgus as raw material, adopts the extracting method of semi continuous, has that extraction yield is high, extraction agent consumption is less, and technique is simple, easy to operate, control.
Description
Technical field
The present invention relates to the apparatus and method that a kind of semi continuous extracts polyporusum bellatus, belong to chemical technology field.
Background technology
Polysaccharide is isolating protein and the nucleic acid biomacromolecule that another class is important outward in organism, have life entity immunologic injury or immunodeficiency disease and many-sided function and the biological activity such as anti-oxidant, as anti-infective, immunological enhancement, treatment and prevention of tumour, treating AIDS and control etc., biologically be called " biological response effector " (biologicalresponsemodifier, BRM) or active polysaccharide.Due to the diversified bioactive functions of polysaccharide and in functional food and widespread use clinically, the explo iting and researching of polysaccharide Biological resources is become increasingly active, becomes the study hotspot of natural drug, biological chemistry, life science.
Umbellate pore furgus (Polyporusumbellatus (Pers.) Fries), has another name called pig Poria cocos, belongs to Holobasidiomycetes, aptychus Zoopagales, polyporaceae, Polyporus.Its dry sclerotia is called umbellate pore furgus, is conventional medicinal fungi, because the pitch-dark shape of appearance is gained the name as pig dung.Umbellate pore furgus pharmaceutical use just has application and records in Ancient Times in China " village " Shennong's Herbal.Umbellate pore furgus taste is sweet, gas is light, property is flat, returns kidney, urinary bladder channel, has effect of promoting diuresis to eliminate damp pathogen.Much research in recent years show umbellate pore furgus except the effect having diuresis in addition anti-ageing, antitumor, protect the liver, immunomodulatory, radioprotective, antimutagenic and the pharmacology such as antibacterial effect, the pharmaceutical use of umbellate pore furgus is more and more subject to people's attention.The polysaccharide (being called for short polyporusum bellatus, PUPS) extracted from umbellate pore furgus has Tumor suppression growth and increases the effect of tumor animal and tumour patient body's immunity, also there are some researches prove that PUPS has regulating effect to LAK cell killing tumor promotion.Since the vital role of polyporusum bellatus is found, along with going deep into of research technique, now existing a small amount of production.But because of its complex process, extraction solvent usage quantity is large, and yield is low, and cost is high, expensive, and turnout is little, seriously constrains application clinically.
Summary of the invention
Technical problem to be solved by this invention provides a kind of semi continuous to extract the apparatus and method of polyporusum bellatus for the deficiencies in the prior art.
Technical scheme of the present invention is as follows:
Semi continuous extracts a device for polyporusum bellatus, comprises extraction agent storage tank (1), extraction agent vacuum primingpump (2), ultrasonic-assisted extraction device (5), force (forcing) pump (6), hollow membrane tripping device (9), concentrated solution collector (10); Clear liquid collector (11); Ultrasonic-assisted extraction device (5) is provided with whipping appts and the symmetrically arranged Glass Fibre Bag stationary installation of surrounding; The molecular weight cut-off of hollow membrane tripping device (9) is 10000; Extraction agent sucks and joins in ultrasonic-assisted extraction device (5) by extraction agent vacuum primingpump (2), ultrasonic-assisted extraction device (5) outlet is connected with hollow membrane tripping device (9) by force (forcing) pump (6), concentrated solution enters concentrated solution collector (10), next step alcohol pending is analysed, is precipitated, and clear liquid enters clear liquid collector (11).
Extract the method for polyporusum bellatus according to described device, umbellate pore furgus is dried to constant weight at 90 DEG C, pulverized 20 mesh sieves, extraction agent is deionized water, regulates Extractant pH to be 6.5, extraction agent is regulated to enter and flow out the flow of ultrasonic-assisted extraction device (5), the ratio control of dosage and umbellate pore furgus particle is extracted 15-20mL: 1g in extraction element, control and make extraction agent flow be 20mL/min, when extraction agent enters consistent with the flow flowing out ultrasonic-assisted extraction device (5), control the heating unit of ultrasonic-assisted extraction device (5), ultrasonic-assisted extraction device (5) interior temperature is made to maintain 50 DEG C, adjustment ultrasonic frequency is 25kHz, the stationary installation inserted by the Glass Fibre Bag that raw material umbellate pore furgus is housed in ultrasonic-assisted extraction device (5) is fixed, open the agitator of ultrasonic-assisted extraction device (5), controlling rotating speed is 100 turns/min, carry out semi continuous and extract polysaccharide, the time for the treatment of reaches 40min, change next batch raw material, the extracting solution flowing out ultrasonic-assisted extraction device (5) is forced into 0.5MPa through force (forcing) pump (6), send into molecular weight cut-off be 10000 hollow membrane tripping device (9) concentrate, obtaining flow is the concentrated solution of 10mL/min, this concentrated solution is collected in concentrated solution collector (10), next step alcohol pending is analysed, is precipitated, and be collected in clear liquid collector (11) from the clear liquid that hollow membrane separation unit (9) flows out, abandon it.
The advantages such as the present invention take umbellate pore furgus as raw material, adopts the extracting method of semi continuous, has that extraction yield is high, extraction agent consumption is less, and technique is simple, easy to operate, control.
Accompanying drawing explanation
Fig. 1 is the apparatus structure schematic diagram that semi continuous of the present invention extracts polysaccharide;
1 extraction agent storage tank, 2 extraction agent vacuum primingpumps, 3 first flow control valves, 4 first adjustable fluid flow gauge, 5 ultrasonic-assisted extraction devices, 6 force (forcing) pumps, 7 second control valves, 8 second adjustable fluid flow gauge, 9 hollow membrane tripping devices, 10 concentrated solution collectors, 11 clear liquid collectors.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
With reference to figure 1, semi continuous extracts the device of polyporusum bellatus, comprises extraction agent storage tank 1, extraction agent vacuum primingpump 2, first flow control valve 3, first adjustable fluid flow gauge 4, ultrasonic-assisted extraction device 5 (including the Glass Fibre Bag stationary installation of whipping appts and surrounding symmetry), force (forcing) pump 6, second control valve 7, second adjustable fluid flow gauge 8, hollow membrane tripping device 9 (molecular weight cut-off 10000), concentrated solution collector 10; Clear liquid collector 11.Extraction agent sucks and joins in ultrasonic-assisted extraction device 5 by extraction agent vacuum primingpump 2, ultrasonic-assisted extraction device 5 is exported and is connected with hollow membrane tripping device 9 by force (forcing) pump 6, concentrated solution enters concentrated solution collector 10, the alcohol carrying out next step is analysed, is precipitated operation, and clear liquid enters clear liquid collector 11;
Dried to constant weight at 90 DEG C by umbellate pore furgus, pulverized 20 mesh sieves, screen over-size takes 60g Glass Fibre Bag encapsulation (Glass Fibre Bag can make extraction agent pass through and particle entrapment smoothly), extraction agent is deionized water, regulates Extractant pH to be 6.5, extraction agent is regulated to enter and flow out the flow of ultrasonic-assisted extraction device 5, making to extract dosage in ultrasonic-assisted extraction device 5 is about 800mL, the ratio control of dosage and umbellate pore furgus particle is extracted 15-20mL: 1g in extraction element, control and make extraction agent flow be 20mL/min, when extraction agent enters consistent with the flow flowing out ultrasonic-assisted extraction device 5, control the heating unit of ultrasonic-assisted extraction device 5, temperature in ultrasonic-assisted extraction device 5 is made to maintain 50 DEG C, adjustment ultrasonic frequency is 25kHz, the stationary installation that the Glass Fibre Bag that raw material is housed is inserted in ultrasonic-assisted extraction device 5 is fixed, the agitator control rotating speed opening ultrasonic-assisted extraction device 5 is 100 turns/min, carry out semi continuous and extract polysaccharide, the time for the treatment of reaches 40min, change next batch raw material, the extracting solution flowing out ultrasonic-assisted extraction device 5 is forced into 0.5MPa through force (forcing) pump 6, send into molecular weight cut-off be 10000 hollow membrane tripping device 9 concentrate, obtaining flow is the concentrated solution of 10mL/min, this concentrated solution is collected in concentrated solution collector 10, treat that next step processes, and be collected in clear liquid collector 11 from the clear liquid that hollow membrane separation unit 9 flows out, abandon it.Polyporusum bellatus yield is 1.283% with this understanding.Carry out large-scale production as strengthened charging capacity, the operating parameters of technical process is all comparable constant except extraction agent flow, and extraction agent flow can be reconciled according to the ratio of the extraction liquid measure in extraction element and extraction time 40min.
This shortcoming that process reform conventional batch polysaccharide extracting process process time is long, energy expenditure is many, extraction agent consumption is large; The extraction process design of this semi continuous polysaccharide simultaneously, the semi continuous being applicable to the sky thermal activities compositions such as all polysaccharide, polyphenol, Flavonoid substances extracts, only according to the structural modification extraction agent type of raw material cell wall, pH, Extracting temperature, extraction agent flow and ultrasonic frequency, the integrated application realizing extraction process is worth.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (1)
1. the method for a semi continuous extraction polyporusum bellatus, it is characterized in that, completed by semi continuous extraction element, described extraction element comprises extraction agent storage tank (1), extraction agent vacuum primingpump (2), ultrasonic-assisted extraction device (5), force (forcing) pump (6), hollow membrane tripping device (9), concentrated solution collector (10); Clear liquid collector (11); Ultrasonic-assisted extraction device (5) is provided with whipping appts and the symmetrically arranged Glass Fibre Bag stationary installation of surrounding; The molecular weight cut-off of hollow membrane tripping device (9) is 10000; Extraction agent sucks and joins in ultrasonic-assisted extraction device (5) by extraction agent vacuum primingpump (2), ultrasonic-assisted extraction device (5) outlet is connected with hollow membrane tripping device (9) by force (forcing) pump (6), concentrated solution enters concentrated solution collector (10), next step alcohol pending is analysed, is precipitated, and clear liquid enters clear liquid collector (11);
Concrete extracting method is: dried to constant weight at 90 DEG C by umbellate pore furgus, pulverized 20 mesh sieves, extraction agent is deionized water, regulates Extractant pH to be 6.5, extraction agent is regulated to enter and flow out the flow of ultrasonic-assisted extraction device (5), the ratio control of dosage and umbellate pore furgus particle is extracted 15 ~ 20mL: 1g in extraction element, control and make extraction agent flow be 20mL/min, when extraction agent enters consistent with the flow flowing out ultrasonic-assisted extraction device (5), control the heating unit of ultrasonic-assisted extraction device (5), ultrasonic-assisted extraction device (5) interior temperature is made to maintain 50 DEG C, adjustment ultrasonic frequency is 25kHz, the stationary installation inserted by the Glass Fibre Bag that raw material umbellate pore furgus is housed in ultrasonic-assisted extraction device (5) is fixed, open the whipping appts of ultrasonic-assisted extraction device (5), controlling rotating speed is 100 turns/min, carry out semi continuous and extract polysaccharide, the time for the treatment of reaches 40min, change next batch raw material, the extracting solution flowing out ultrasonic-assisted extraction device (5) is forced into 0.5MPa through force (forcing) pump (6), send into molecular weight cut-off be 10000 hollow membrane tripping device (9) concentrate, obtaining flow is the concentrated solution of 10mL/min, this concentrated solution is collected in concentrated solution collector (10), next step alcohol pending is analysed, is precipitated, and be collected in clear liquid collector (11) from the clear liquid that hollow membrane separation unit (9) flows out, abandon it.
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| CN108456260A (en) * | 2018-05-15 | 2018-08-28 | 重庆市洲仨科技发展有限公司 | Plant polyose extracts separating and processing apparatus |
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| CN101879380A (en) * | 2010-06-25 | 2010-11-10 | 戴群 | Energy-saving concentration process for extracting active ingredients of plants and concentration device thereof |
| CN201990619U (en) * | 2011-03-16 | 2011-09-28 | 厦门市威士邦膜科技有限公司 | Lentinan extraction, separation, purification and concentration device |
| CN102198049A (en) * | 2011-05-10 | 2011-09-28 | 浙江中医药大学 | Traditional Chinese medicine preparation method and device |
| CN102276752A (en) * | 2011-09-13 | 2011-12-14 | 陕西理工学院 | Device and method for extracting polysaccharides from agaric polysaccharide extracting solution by foam fractionation |
| CN202610144U (en) * | 2012-04-28 | 2012-12-19 | 华南理工大学 | Continuous polysaccharide degradation device coupling power ultrasound and membrane separation methods |
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Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1682751A (en) * | 2005-02-25 | 2005-10-19 | 张海峰 | Agaric polysaccharide freeze-dried powder injection and its preparing method |
| CN1721028A (en) * | 2005-05-18 | 2006-01-18 | 戴群 | Integrated membrane circulation extracting apparatus |
| CN101851297A (en) * | 2010-05-06 | 2010-10-06 | 安徽天品堂生态农业有限公司 | High-efficiency extraction process of polygahatous plysaccharides |
| CN101879380A (en) * | 2010-06-25 | 2010-11-10 | 戴群 | Energy-saving concentration process for extracting active ingredients of plants and concentration device thereof |
| CN201990619U (en) * | 2011-03-16 | 2011-09-28 | 厦门市威士邦膜科技有限公司 | Lentinan extraction, separation, purification and concentration device |
| CN102198049A (en) * | 2011-05-10 | 2011-09-28 | 浙江中医药大学 | Traditional Chinese medicine preparation method and device |
| CN102276752A (en) * | 2011-09-13 | 2011-12-14 | 陕西理工学院 | Device and method for extracting polysaccharides from agaric polysaccharide extracting solution by foam fractionation |
| CN202610144U (en) * | 2012-04-28 | 2012-12-19 | 华南理工大学 | Continuous polysaccharide degradation device coupling power ultrasound and membrane separation methods |
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