CN103232553A - Device and method for semi-continuous extraction of polyporus umbellatus polysaccharides - Google Patents
Device and method for semi-continuous extraction of polyporus umbellatus polysaccharides Download PDFInfo
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- CN103232553A CN103232553A CN 201310171854 CN201310171854A CN103232553A CN 103232553 A CN103232553 A CN 103232553A CN 201310171854 CN201310171854 CN 201310171854 CN 201310171854 A CN201310171854 A CN 201310171854A CN 103232553 A CN103232553 A CN 103232553A
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Abstract
The invention discloses a device and method for semi-continuous extraction of polyporus umbellatus polysaccharides. The device comprises an extraction agent storage tank (1), an extraction agent self-suction pump (2), an ultrasonic-assisted extraction device (5), a pressurization pump (6), a hollow membrane separation device (9), a concentrated liquid collector (10) and a clear liquid collector (11), wherein the molecular weight cutoff of the hollow membrane separation device (9) is 10000; and the extraction agent self-suction pump (2) is used for sucking an extraction agent and adding the extraction agent into the ultrasonic-assisted extraction device (5), an outlet of the ultrasonic-assisted extraction device (5) is connected with the hollow membrane separation device (9) through the pressurization pump (6), concentrated liquid enters the concentrated liquid collector (10), next-step alcohol precipitation and sedimentation are further performed, and clear liquid enters the clear liquid collector (11). According to the invention, polyporus umbellatus is taken as a raw material, a semi-continuous extraction method is adopted, and the device has the advantages of high extraction rate, smaller using quantity of the extraction agent, simple process, easiness in operation and control and the like.
Description
Technical field
The present invention relates to the apparatus and method that a kind of semi continuous extracts polyporusum bellatus, belong to chemical technology field.
Background technology
Polysaccharide is isolating protein and the outer important biomacromolecule of another class of nucleic acid in the organism, have life entity immunologic injury or immunodeficiency disease and many-sided function and biological activity such as anti-oxidant, as anti-infective, immunological enhancement, treatment and prevention of tumour, treating AIDS and control etc., biologically be called " biological response effector " (biological response modifier, BRM) or active polysaccharide.Owing to the diversified bioactive functions of polysaccharide and in functional food and widespread use clinically, the utilization of polysaccharide The biological resources development and research are become increasingly active, become the research focus of natural drug, biological chemistry, life science.
Umbellate pore furgus (Polyporus umbellatus (Pers.) Fries) has another name called the pig Poria cocos, belongs to Holobasidiomycetes, aptychus Zoopagales, polyporaceae, Polyporus.Its dry sclerotia is called umbellate pore furgus, is medicinal fungi commonly used, because the pitch-dark shape of appearance such as pig dung are gained the name.The umbellate pore furgus pharmaceutical use just has in Ancient Times in China " village " Shennong's Herbal to be used and record.The umbellate pore furgus flavor is sweet, gas is light, the property is flat, returns kidney, urinary bladder channel, and the effect of promoting diuresis to eliminate damp pathogen is arranged.In recent years many studies show that umbellate pore furgus except diuretic actions is arranged in addition anti-ageing, antitumor, protect the liver, the effect of immunomodulatory, radioprotective, antimutagenic and pharmacology such as antibiotic, the pharmaceutical use of umbellate pore furgus more and more is subject to people's attention.The polysaccharide that extracts from umbellate pore furgus (is called for short polyporusum bellatus, PUPS) has the effect that suppresses tumor growth and increase tumor animal and tumour patient body's immunity, there are some researches prove that also the LAK cell killing of PUPS tumor promotion has regulating effect.Since the vital role of polyporusum bellatus is found, along with going deep into of research technology, existing a spot of production now.But because of its complex process, the extraction solvent usage quantity is big, and yield is low, the cost height, and expensive, turnout is little, has seriously restricted application clinically.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of semi continuous to extract the apparatus and method of polyporusum bellatus at the deficiencies in the prior art.
Technical scheme of the present invention is as follows:
A kind of semi continuous extracts the device of polyporusum bellatus, comprises extraction agent storage tank (1), extraction agent vacuum primingpump (2), ultrasonic-assisted extraction device (5), force (forcing) pump (6), hollow membrane tripping device (9), concentrated solution collector (10); Clear liquid collector (11); Ultrasonic-assisted extraction device (5) is provided with whipping appts and symmetrically arranged Glass Fibre Bag stationary installation all around; The molecular weight cut-off of hollow membrane tripping device (9) is 10000; Extraction agent vacuum primingpump (2) sucks extraction agent and join in the ultrasonic-assisted extraction device (5), ultrasonic-assisted extraction device (5) outlet is connected with hollow membrane tripping device (9) by force (forcing) pump (6), concentrated solution enters concentrated solution collector (10), pending next step alcohol is analysed, is precipitated, and clear liquid enters clear liquid collector (11).
According to the method for described device extraction polyporusum bellatus, umbellate pore furgus is dried to constant weight at 90 ℃, pulverized 20 mesh sieves; Extraction agent is deionized water, and regulating extraction agent pH is 6.5; Regulate the flow that extraction agent entered and flowed out ultrasonic-assisted extraction device (5), extract dosage and the control of umbellate pore furgus proportion of particles in the extraction element at 15-20mL: 1g, controlling and making the extraction agent flow is 20mL/min, when extraction agent enters when consistent with the flow that flows out ultrasonic-assisted extraction device (5), the heating unit of control ultrasonic-assisted extraction device (5), make the interior temperature maintenance of ultrasonic-assisted extraction device (5) at 50 ℃, the adjusting ultrasonic frequency is 25kHz, the stationary installation that the Glass Fibre Bag that the raw material umbellate pore furgus is housed is inserted in the ultrasonic-assisted extraction device (5) is fixed, open the agitator of ultrasonic-assisted extraction device (5), the control rotating speed is 100 commentaries on classics/min, carry out semi continuous and extract polysaccharide, the time for the treatment of reaches 40min, changes the next batch raw material; The extracting solution that flows out ultrasonic-assisted extraction device (5) is forced into 0.5MPa through force (forcing) pump (6), sending into molecular weight cut-off and be 10000 hollow membrane tripping device (9) concentrates, getting flow is the concentrated solution of 10mL/min, this concentrated solution is collected in the concentrated solution collector (10), pending next step alcohol is analysed, is precipitated, and the clear liquid that flows out from hollow membrane separation unit (9) is collected in clear liquid collector (11), abandons it.
The present invention is raw material with the umbellate pore furgus, adopts the extracting method of semi continuous, has the extraction yield height, the extraction agent consumption is less, and technology is simple, advantages such as easy to operate, control.
Description of drawings
Fig. 1 is the apparatus structure synoptic diagram that semi continuous of the present invention extracts polysaccharide;
1 extraction agent storage tank, 2 extraction agent vacuum primingpumps, 3 first flow control valves, 4 first adjustable flow meters, 5 ultrasonic-assisted extraction devices, 6 force (forcing) pumps, 7 second flowrate control valves, 8 second adjustable flow meters, 9 hollow membrane tripping devices, 10 concentrated solution collectors, 11 clear liquid collectors.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
With reference to figure 1, semi continuous extracts the device of polyporusum bellatus, comprises extraction agent storage tank 1, extraction agent vacuum primingpump 2, first flow control valve 3, the first adjustable flow meter 4, ultrasonic-assisted extraction device 5 (include whipping appts and symmetrical Glass Fibre Bag stationary installation) all around, force (forcing) pump 6, second flowrate control valve 7, the second adjustable flow meter 8, hollow membrane tripping device 9 (molecular weight cut-off 10000), concentrated solution collector 10; Clear liquid collector 11.Extraction agent vacuum primingpump 2 sucks extraction agent and join in the ultrasonic-assisted extraction device 5,5 outlets of ultrasonic-assisted extraction device are connected with hollow membrane tripping device 9 by force (forcing) pump 6, concentrated solution enters concentrated solution collector 10, operation is analysed, precipitated to the alcohol that carries out next step, and clear liquid enters clear liquid collector 11;
Umbellate pore furgus is dried to constant weight at 90 ℃, pulverized 20 mesh sieves, screen over-size take by weighing 60g with Glass Fibre Bag encapsulation (Glass Fibre Bag can make extraction agent smoothly by and particle hold back); Extraction agent is deionized water, and regulating extraction agent pH is 6.5; Regulate the flow that extraction agent entered and flowed out ultrasonic-assisted extraction device 5, make that ultrasonic-assisted extraction device 5 interior extraction dosage are about 800mL, extract dosage and the control of umbellate pore furgus proportion of particles in the extraction element at 15-20mL: 1g, controlling and making the extraction agent flow is 20mL/min, when extraction agent enters when consistent with the flow that flows out ultrasonic-assisted extraction device 5, the heating unit of control ultrasonic-assisted extraction device 5, make ultrasonic-assisted extraction device 5 interior temperature maintenance at 50 ℃, the adjusting ultrasonic frequency is 25kHz, the stationary installation that the Glass Fibre Bag that raw material is housed is inserted in the ultrasonic-assisted extraction device 5 is fixed, the agitator control rotating speed of opening ultrasonic-assisted extraction device 5 is 100 commentaries on classics/min, carry out semi continuous and extract polysaccharide, the time for the treatment of reaches 40min, changes the next batch raw material; The extracting solution that flows out ultrasonic-assisted extraction device 5 is forced into 0.5MPa through force (forcing) pump 6, sending into molecular weight cut-off and be 10000 hollow membrane tripping device 9 concentrates, getting flow is the concentrated solution of 10mL/min, this concentrated solution is collected in the concentrated solution collector 10, treat next step processing, and the clear liquid that flows out from hollow membrane separation unit 9 is collected in clear liquid collector 11, abandons it.The polyporusum bellatus yield is 1.283% with this understanding.Strengthen charging capacity as need and carry out large-scale production, the operating parameters of technical process is all comparable constant except the extraction agent flow, and the extraction agent flow can be reconciled according to the ratio of the extraction liquid measure in the extraction element and extraction time 40min and got final product.
The shortcoming that this process reform traditional batch formula polysaccharide extracting process process time is long, energy expenditure is many, the extraction agent consumption is big; The extraction process of this semi continuous polysaccharide design simultaneously, semi continuous applicable to day hot activeconstituents such as all polysaccharide, polyphenol, Flavonoid substances extracts, only structural modification extraction agent type, pH, extraction temperature, extraction agent flow and the ultrasonic frequency according to raw material cell wall gets final product, and realizes that the integrated application of extraction process is worth.
Should be understood that, for those of ordinary skills, can be improved according to the above description or conversion, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (2)
1. the device of a semi continuous extraction polyporusum bellatus is characterized in that, comprises extraction agent storage tank (1), extraction agent vacuum primingpump (2), ultrasonic-assisted extraction device (5), force (forcing) pump (6), hollow membrane tripping device (9), concentrated solution collector (10); Clear liquid collector (11); Ultrasonic-assisted extraction device (5) is provided with whipping appts and symmetrically arranged Glass Fibre Bag stationary installation all around; The molecular weight cut-off of hollow membrane tripping device (9) is 10000; Extraction agent vacuum primingpump (2) sucks extraction agent and join in the ultrasonic-assisted extraction device (5), ultrasonic-assisted extraction device (5) outlet is connected with hollow membrane tripping device (9) by force (forcing) pump (6), concentrated solution enters concentrated solution collector (10), pending next step alcohol is analysed, is precipitated, and clear liquid enters clear liquid collector (11).
2. device according to claim 1 extracts the method for polyporusum bellatus, it is characterized in that, umbellate pore furgus is dried to constant weight at 90 ℃, pulverizes 20 mesh sieves; Extraction agent is deionized water, and regulating extraction agent pH is 6.5; Regulate the flow that extraction agent entered and flowed out ultrasonic-assisted extraction device (5), extract dosage and the control of umbellate pore furgus proportion of particles in the extraction element at 15-20mL: 1g, controlling and making the extraction agent flow is 20mL/min, when extraction agent enters when consistent with the flow that flows out ultrasonic-assisted extraction device (5), the heating unit of control ultrasonic-assisted extraction device (5), make the interior temperature maintenance of ultrasonic-assisted extraction device (5) at 50 ℃, the adjusting ultrasonic frequency is 25kHz, the stationary installation that the Glass Fibre Bag that the raw material umbellate pore furgus is housed is inserted in the ultrasonic-assisted extraction device (5) is fixed, open the agitator of ultrasonic-assisted extraction device (5), the control rotating speed is 100 commentaries on classics/min, carry out semi continuous and extract polysaccharide, the time for the treatment of reaches 40min, changes the next batch raw material; The extracting solution that flows out ultrasonic-assisted extraction device (5) is forced into 0.5MPa through force (forcing) pump (6), sending into molecular weight cut-off and be 10000 hollow membrane tripping device (9) concentrates, getting flow is the concentrated solution of 10mL/min, this concentrated solution is collected in the concentrated solution collector (10), pending next step alcohol is analysed, is precipitated, and the clear liquid that flows out from hollow membrane separation unit (9) is collected in clear liquid collector (11), abandons it.
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| CN108456260A (en) * | 2018-05-15 | 2018-08-28 | 重庆市洲仨科技发展有限公司 | Plant polyose extracts separating and processing apparatus |
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| CN1682751A (en) * | 2005-02-25 | 2005-10-19 | 张海峰 | Agaric polysaccharide freeze-dried powder injection and its preparing method |
| CN1298406C (en) * | 2005-05-18 | 2007-02-07 | 戴群 | Integrated membrane circulation extracting apparatus |
| CN101851297A (en) * | 2010-05-06 | 2010-10-06 | 安徽天品堂生态农业有限公司 | High-efficiency extraction process of polygahatous plysaccharides |
| CN101879380B (en) * | 2010-06-25 | 2012-07-18 | 戴群 | Energy-saving concentration process for extracting active ingredients of plants and concentration device thereof |
| CN201990619U (en) * | 2011-03-16 | 2011-09-28 | 厦门市威士邦膜科技有限公司 | Lentinan extraction, separation, purification and concentration device |
| CN102198049A (en) * | 2011-05-10 | 2011-09-28 | 浙江中医药大学 | Traditional Chinese medicine preparation method and device |
| CN102276752B (en) * | 2011-09-13 | 2013-06-05 | 陕西理工学院 | Device and method for extracting polysaccharides from agaric polysaccharide extracting solution by foam fractionation |
| CN202610144U (en) * | 2012-04-28 | 2012-12-19 | 华南理工大学 | Continuous polysaccharide degradation device coupling power ultrasound and membrane separation methods |
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| CN108456260A (en) * | 2018-05-15 | 2018-08-28 | 重庆市洲仨科技发展有限公司 | Plant polyose extracts separating and processing apparatus |
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