CN1682751A - Agaric polysaccharide freeze-dried powder injection and its preparing method - Google Patents
Agaric polysaccharide freeze-dried powder injection and its preparing method Download PDFInfo
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Abstract
The present invention relates to Chinese medicine preparing technology, and is especially freeze dried agaric polysaccharide powder for injection. The freeze dried agaric polysaccharide powder for injection is prepared with agaric polysaccharide with molecular weight of 5,000-40,000 and water soluble supplementary material for injection. The present invention also relates to the preparation process of the said powder for injection. The present invention prepares agaric polysaccharide extract with high active polysaccharide content, and has preparation process with short period, less steps and low cost and suitable for industrial production.
Description
Technical field
The invention belongs to technical field of traditional Chinese medicine pharmacy, be specifically related to a kind of polyporusum bellatus lyophilized injectable powder and preparation method thereof.
Background technology
Polyporusum bellatus is the effective ingredient that extracts among Polyporaceae fungus Polyporus Polyporusumbellatus (Pers) Fries, and it has the effect of regulating body's immunity, and chronic hepatitis, oncosis are had certain curative effect.With polyporusum bellatus is that the preparation of main effective ingredient has polyporusum bellatus capsule [application number is the patent documentation of CN93101212], ZHULINGDUOTANG ZHUSHEYE and polyporusum bellatus lyophilized injectable powder.The preparation method of Polyporus injection [18 in Chinese medicine promulgated by the ministries or commissions of the Central Government] is as follows: the method that water extract-alcohol precipitation is twice obtains the polyporusum bellatus crude product, crude product obtains the pure product of polyporusum bellatus with the method for tannic acid processing, charcoal treatment, ethanol precipitation, mistake neutral alumina, ethanol precipitation, is mixed with injection again; The preparation method of polyporusum bellatus lyophilized injectable powder [application number is the patent documentation of CN95115855] is as follows: twice of water extract-alcohol precipitation, mistake yin, yang resin column, and cross sephadex column, microporous filter membrane or ultrafiltration, obtain polyporusum bellatus with spray drying or lyophilization.Said method all adopts classical water extract-alcohol precipitation method to obtain polyporusum bellatus, but contains very big deficiency: hot water extraction, though extracting efficiency of polysaccharides is higher, but the amount of hot water dissolving's impurity also increases thereupon; The increase of impurity level makes the unusual complexity of follow-up impurity removal process, loaded down with trivial details; The complexity of technology, loaded down with trivial detailsly will make that effective ingredient suffers a loss in the process of remove impurity; Its result makes very low of extraction ratio of the polysaccharide that finally obtains from the Polyporus medical material.This is not profuse Polyporus medical material for resource, is a kind of very big waste; And extraction efficiency is low, can make that the cost of the ejection preparation made is very high, for the patient, also is a kind of burden; Except the active polyporusum bellatus composition that contains the particular range molecular weight, also contain the macromole and the micromolecule composition of other non-activity in the extract, so do not reach real " discarding the dross and selecting the essential ".
Summary of the invention
The objective of the invention is to disclose a kind of polyporusum bellatus lyophilized injectable powder.
Another object of the present invention is the preparation method that discloses above-mentioned injectable powder.
The present invention realizes by following scheme.
One, preparation method
(1) gets the Polyporus medical material, pulverize, cross 40 mesh sieves, add 8-14 times of water gaging, soak half an hour, put into the supersound extraction jar, carry out supersound extract, the time is 30-60 minute, and frequency of oscillation is 20-80kHz, extract 3-4 time, merge extractive liquid, filters, and filtrate decompression is condensed into extractum;
(2) above-mentioned extractum is added 30-40 and doubly measure 60 ℃-80 ℃ water, stir, leave standstill a moment, inclining supernatant, is evaporated to solution: medical material is 1: 1, adds 95% ethanol and makes that containing pure amount is 70%-90%, leaves standstill 24 hours, filters, and precipitates standby;
(3) above-mentioned precipitation is dissolved with 20-30 times of water gaging, heat when under agitation dripping 1% tannic acid, boil the centrifugal disgorging in back, continue to add tannic acid to solution and do not produce muddiness, cross the polyamide column of having handled well,, collect water lotion with 4-8 water elution doubly, with molecular cut off is the ultrafilter membrane ultrafiltration of 30000-40000, filtrate reuse molecular cut off is the ultrafilter membrane ultrafiltration of 5000-8000, collects not by liquid concentrating under reduced pressure, drying promptly gets polyporusum bellatus;
(4) take by weighing above-mentioned polyporusum bellatus, add the water solublity freeze-dried excipient, regulating pH value with meglumine is 7.0-8.5, and with 0.22 μ m filtering with microporous membrane, lyophilization is packaged to be freeze-dried powder of the present invention.
Ultrasound assisted extraction technique is to utilize ultrasound wave to produce judder, and is high-speed, intensive cavitation effect, and stirring action can destroy the cell of medical material, make the solvent porous in crude drug cell, thereby the effective ingredient that quickens in the medical material is dissolved in the solvent.Ultrasonic technique extraction rate height, extraction time be short, it is low to extract temperature, in preparation method of the present invention to the extraction of Polyporus medical material, can be so that polyporusum bellatus effective component extraction rate height; Temperature is low, has lacked much compared with decocting for the extracted amount of impurity, has come method for follow-up remove impurity process.
It is classic methods that tannic acid removes albumen, but the amount of tannic acid is wayward.Tannic acid adds very few, and is incomplete to proteic precipitation, can make the safety of medicine descend; Add too much, if do not remove, patient's pain in the time of can increasing injection.Polyamide has good adsorption for tannic acid, and polyporusum bellatus is not then had adsorption.Utilize this point, can remove the impurity that excessive tannic acid that protein precipitation adds and some can be adsorbed by polyamide, polyporusum bellatus is further purified.
Hyperfiltration technique is meant under the room temperature with certain pressure and flow, utilize asymmetric microcellular structure and semipermeable membrane separating medium, filter in the cross-flow mode, solvent and small-molecule substance are passed through, polymer substance is by the filter membrane detention, thereby reaches separation, purification, spissated new membrane isolation technics with fastest developing speed.Compare with traditional separation method, have separation process do not have phase transformation, separation efficiency height, need not to add chemical reagent pollution-free, need not to heat, energy consumption is low, mild condition is not destroyed composition, easy to operate, characteristics that flow process is short.The present invention utilizes hyperfiltration technique, selects suitable ultrafilter membrane, has removed the macromole and the small-molecule substance of non-activity, is further purified having active polyporusum bellatus.
Two, the comparison of preparation method extraction ratio
For science, reasonability that preparation method of the present invention is described, research worker of the present invention has been carried out following comparative experiments.
To be divided into several parts with a collection of Polyporus medical material, every part of 5kg is prepared into 1,2, No. 3 following sample.
No. 1 polyporusum bellatus sample: by the preparation of the method in the ZHULINGDUOTANG ZHUSHEYE in 18 in the Chinese medicine promulgated by the ministries or commissions of the Central Government; No. 2 polyporusum bellatus samples: be the method preparation in the patent documentation of CN95115855 by application number; No. 3 polyporusum bellatus samples: by preparation method preparation of the present invention.Provide by Tianzhijiao Medication Development Co., Ltd., Guangdong.
Content assaying method carries out according to the spectrophotography in the assay under the ZHULINGDUOTANG ZHUSHEYE item in 18 in the Chinese medicine promulgated by the ministries or commissions of the Central Government.The content of polysaccharide is 0.76% (g/g) in the Polyporus medical material.The results are shown in Table 1.
The comparison of table 1 preparation method extraction ratio
| Lot number | Extraction ratio (%) |
| No. 1 | No. 2 | No. 3 | |
| ????1 ????2 ????3 ????4 ????5 | ??44.5 ??42.3 ??40.8 ??43.5 ??40.4 | ??53.4 ??49.8 ??52.7 ??52.2 ??50.6 | ??74.6 ??76.2 ??77.0 ??73.8 ??75.4 |
By the experimental data of table 1 as can be seen, when the raw medicinal material of same amount, the polyporusum bellatus extraction ratio of preparation method of the present invention has the raising of significance than the extraction ratio of other two kinds of methods, and this proves absolutely preparation method of the present invention science, rationally more.
Three, assay
For the content of the polyporusum bellatus that confirms to have in the polyporusum bellatus that preparation method of the present invention obtains active component is higher, research worker of the present invention has been carried out assay to above-mentioned No. 1, No. 2, No. 3 samples.
Algoscopy: precision takes by weighing each 50mg above-mentioned No. 1, No. 2, No. 3, puts in the 50ml measuring bottle, adds water to scale, shakes up.Precision is measured 1ml and is put in the 50ml measuring bottle, adds water and puts scale, shakes up.With solution is respectively 40000 ultrafilter membrane by molecular cut off, and the filtrate of collection is 5000 ultrafilter membrane again by molecular cut off, collects not by liquid in the 50ml measuring bottle, and thin up shakes up to scale.Below measure according to the content assaying method in the ZHULINGDUOTANG ZHUSHEYE in 18 in the Chinese medicine promulgated by the ministries or commissions of the Central Government.The results are shown in Table 2.
Table 2 assay result's comparison
| Sample | No. 1 | No. 2 | No. 3 |
| Content (%) | ??89.6 | ??92.2 | ??98.7 |
By above assay result as can be seen: the content of active polysaccharide is apparently higher than other two kinds of polyporusum bellatuss that method obtains in the polyporusum bellatus that preparation method of the present invention obtains, and this proves absolutely preparation method of the present invention science, reasonable more.
Four, the comparison of preparation method output
The specification of ZHULINGDUOTANG ZHUSHEYE is that 20mg/ props up in 18 of the ministry standards.For the ease of comparison and drug safety, above-mentioned 1,2, No. 3 sample that obtains also is mixed with 20mg/ props up, also be prepared into corresponding dosage forms.Provide by Tianzhijiao Medication Development Co., Ltd., Guangdong.The results are shown in Table 3.
The comparison of table 3 preparation method output
| Lot number | Output (propping up) | ||
| No. 1 | No. 2 | No. 3 | |
| ??1 ??2 ??3 ??4 ??5 | ????820 ????785 ????760 ????800 ????750 | ??1000 ??930 ??980 ??980 ??950 | ??1400 ??1420 ??1450 ??1380 ??1410 |
By table 3 data as can be seen, at the Polyporus medical material of same amount, under the identical prerequisite of preparation specification content, the quantity of the polyporusum bellatus lyophilized injectable powder that preparation method of the present invention is made is far longer than the quantity of the preparation that other two kinds of methods obtain.This further specifies preparation method of the present invention conservation medicine greatly, reduces the medicine cost.
Sum up: by above experiment as can be seen, preparation method of the present invention for the extraction ratio of polyporusum bellatus extraction ratio, active polysaccharide in the Polyporus medical material all apparently higher than other method.For the safety of medication and effectively, under the identical situation of medicine specification, the output of the polyporusum bellatus lyophilized injectable powder that preparation method of the present invention obtains is far longer than the output of other method, this has reduced the medicine production cost virtually, all be very useful for manufacturer, patient, the preparation method that polyporusum bellatus lyophilized injectable powder of the present invention is described is a science, rational.The preparation method of polyporusum bellatus lyophilized injectable powder of the present invention is easy to realize under existing technical merit, and can realize automatization fully, compared with other above-mentioned two kinds of production methods, the production method cycle of the present invention is short, step is few, and consumption of raw materials is few, illustrates that the preparation method of polyporusum bellatus lyophilized injectable powder of the present invention is successful for insufficient improvement in the said method, reasonably, science.
Five, pharmacology embodiment
In order to confirm the therapeutic effect of polyporusum bellatus lyophilized injectable powder of the present invention, research worker of the present invention has been carried out pharmacodynamics test to polyporusum bellatus lyophilized injectable powder of the present invention.
Polyporusum bellatus lyophilized injectable powder of the present invention (specification is that 20mg/ props up) is provided by Tianzhijiao Medication Development Co., Ltd., Guangdong; Commercially available ZHULINGDUOTANG ZHUSHEYE (specification is that 20mg/2ml/ props up) is produced by Jiangsu Zhengda Tianqing Drug Industry Co., Ltd.
Tumor growth in vivo suppresses experiment and selects 30 of Male Kunming strain mice, 18-22g for use.The H that inoculation is back 7 days
22The dislocation of tumor strain tumor-bearing mice cervical vertebra is put to death, and takes out ascites under aseptic condition, with normal saline dilution in 1: 3, in axillary fossa subcutaneous vaccination tumor liquid 0.2ml.Inoculate back 24 hours, animal is divided into 4 groups at random, 10 every group.If the blank group, ZHULINGDUOTANG ZHUSHEYE group, polyporusum bellatus lyophilized injectable powder low dose group of the present invention, polyporusum bellatus lyophilized injectable powder high dose group of the present invention.Negative control group gives isopyknic normal saline, ZHULINGDUOTANG ZHUSHEYE group and polyporusum bellatus lyophilized injectable powder high dose group of the present invention are according to the dosage intraperitoneal injection of 20mg/kg, and polyporusum bellatus lyophilized injectable powder low dose group of the present invention is according to the dosage intraperitoneal injection of 15mg/kg.Every day 1 time, successive administration 10 days.After the last administration, animal is put to death in cervical vertebra dislocation, weighs respectively, tumor is heavy, calculates tumor control rate, carries out therapeutic evaluation.
Inhibition rate of tumor growth (the %)=average tumor of (it is heavy that average tumor is organized in the average tumor weight-treatment of matched group)/matched group heavy * 100%
Experimental result sees Table 4.
Table 4 couple rat liver cancer H
22The inhibitory action of growth (X ± S)
| Group | Mus is (only) only | Tumor heavy (g) | Suppression ratio (%) |
| Blank group ZHULINGDUOTANG ZHUSHEYE group | ????10 ????10 | ?2.42±0.80 ?1.12±0.52 | ????- ????53.7 ** |
| Polyporusum bellatus of the present invention | Low dose group | ????10 | ????1.14±0.48 | ????52.9 ** |
| The lyophilized injectable powder group | High dose group | ????10 | ????0.98±0.36 | ????59.5 **# |
Annotate: compare with matched group:
*P＜0.05,
*P＜0.01;
Compare with the ZHULINGDUOTANG ZHUSHEYE group: #P＜0.05.
By table 4 experimental result as can be seen: polyporusum bellatus lyophilized injectable powder of the present invention and ZHULINGDUOTANG ZHUSHEYE all can suppress rat liver cancer H
22The effect of growth relatively has the difference of significance with matched group; And when dosage is identical, polyporusum bellatus lyophilized injectable powder of the present invention for rat liver cancer H
22The suppression ratio of growth is greater than ZHULINGDUOTANG ZHUSHEYE.The therapeutic effect that polyporusum bellatus lyophilized injectable powder of the present invention is described is better.
Five, preparation embodiment
Embodiment 1
(1) get the Polyporus medical material, pulverize, cross 40 mesh sieves, add 14 times of water gagings, soak half an hour, put into the supersound extraction jar, carry out supersound extract, the time is 60 minutes, and frequency of oscillation is 20kHz, extracts 4 times, and merge extractive liquid, filters, and filtrate decompression is condensed into extractum;
(2) with the water of 80 ℃ of 40 times of amounts of above-mentioned extractum adding, stir, leave standstill a moment, inclining supernatant, is evaporated to solution: medical material is 1: 1, adds 95% ethanol and makes that containing pure amount is 90%, leaves standstill 24 hours, filters, and precipitates standby;
(3) above-mentioned precipitation is dissolved with 30 times of water gagings, heat when under agitation dripping 1% tannic acid, boil the centrifugal disgorging in back, continue to add tannic acid to solution and do not produce muddiness, cross the polyamide column of having handled well,, collect water lotion with 4 times water elution, it with molecular cut off 30000 ultrafilter membrane ultrafiltration, filtrate reuse molecular cut off is 5000 ultrafilter membrane ultrafiltration, collects not by liquid concentrating under reduced pressure, drying promptly gets polyporusum bellatus;
(4) take by weighing above-mentioned polyporusum bellatus 20g, add mannitol, regulating pH value with meglumine is 7.0, and with 0.22 μ m filtering with microporous membrane, lyophilization is packaged to be 1000 of freeze-dried powders of the present invention.
Embodiment 2
(1) get the Polyporus medical material, pulverize, cross 40 mesh sieves, add 8 times of water gagings, soak half an hour, put into the supersound extraction jar, carry out supersound extract, the time is 30 minutes, and frequency of oscillation is 80kHz, extracts 3 times, and merge extractive liquid, filters, and filtrate decompression is condensed into extractum;
(2) with the water of 60 ℃ of 30 times of amounts of above-mentioned extractum adding, stir, leave standstill a moment, inclining supernatant, is evaporated to solution: medical material is 1: 1, adds 95% ethanol and makes that containing pure amount is 70%, leaves standstill 24 hours, filters, and precipitates standby;
(3) above-mentioned precipitation is dissolved with 20 times of water gagings, heat when under agitation dripping 1% tannic acid, boil the centrifugal disgorging in back, continue to add tannic acid to solution and do not produce muddiness, cross the polyamide column of having handled well,, collect water lotion with 8 times water elution, it with molecular cut off 40000 ultrafilter membrane ultrafiltration, filtrate reuse molecular cut off is 8000 ultrafilter membrane ultrafiltration, collects not by liquid concentrating under reduced pressure, drying promptly gets polyporusum bellatus;
(4) take by weighing above-mentioned polyporusum bellatus 20g, add polyvinylpyrrolidone, regulating pH value with meglumine is 8.5, and with 0.22 μ m filtering with microporous membrane, lyophilization is packaged to be 1000 of freeze-dried powders of the present invention.
Embodiment 3
(1) get the Polyporus medical material, pulverize, cross 40 mesh sieves, add 10 times of water gagings, soak half an hour, put into the supersound extraction jar, carry out supersound extract, the time is 50 minutes, and frequency of oscillation is 60kHz, extracts 3 times, and merge extractive liquid, filters, and filtrate decompression is condensed into extractum;
(2) with the water of 70 ℃ of 35 times of amounts of above-mentioned extractum adding, stir, leave standstill a moment, inclining supernatant, is evaporated to solution: medical material is 1: 1, adds 95% ethanol and makes that containing pure amount is 80%, leaves standstill 24 hours, filters, and precipitates standby;
(3) above-mentioned precipitation is dissolved with 25 times of water gagings, heat when under agitation dripping 1% tannic acid, boil the centrifugal disgorging in back, continue to add tannic acid to solution and do not produce muddiness, cross the polyamide column of having handled well,, collect water lotion with 6 times water elution, it with molecular cut off 35000 ultrafilter membrane ultrafiltration, filtrate reuse molecular cut off is 6000 ultrafilter membrane ultrafiltration, collects not by liquid concentrating under reduced pressure, drying promptly gets polyporusum bellatus;
(4) take by weighing above-mentioned polyporusum bellatus 20g, add glucosan, regulating pH value with meglumine is 7.5, and with 0.22 μ m filtering with microporous membrane, lyophilization is packaged to be 1000 of freeze-dried powders of the present invention.
Embodiment 4
(1) get the Polyporus medical material, pulverize, cross 40 mesh sieves, add 12 times of water gagings, soak half an hour, put into the supersound extraction jar, carry out supersound extract, the time is 45 minutes, and frequency of oscillation is 40kHz, extracts 4 times, and merge extractive liquid, filters, and filtrate decompression is condensed into extractum;
(2) with the water of 75 ℃ of 37 times of amounts of above-mentioned extractum adding, stir, leave standstill a moment, inclining supernatant, is evaporated to solution: medical material is 1: 1, adds 95% ethanol and makes that containing pure amount is 85%, leaves standstill 24 hours, filters, and precipitates standby;
(3) above-mentioned precipitation is dissolved with 22 times of water gagings, heat when under agitation dripping 1% tannic acid, boil the centrifugal disgorging in back, continue to add tannic acid to solution and do not produce muddiness, cross the polyamide column of having handled well,, collect water lotion with 5 times water elution, it with molecular cut off 38000 ultrafilter membrane ultrafiltration, filtrate reuse molecular cut off is 7000 ultrafilter membrane ultrafiltration, collects not by liquid concentrating under reduced pressure, drying promptly gets polyporusum bellatus;
(4) take by weighing above-mentioned polyporusum bellatus 20g, add fructose, regulating pH value with meglumine is 8.0, and with 0.22 μ m filtering with microporous membrane, lyophilization is packaged to be 1000 of freeze-dried powders of the present invention.
Embodiment 5
(1) get the Polyporus medical material, pulverize, cross 40 mesh sieves, add 11 times of water gagings, soak half an hour, put into the supersound extraction jar, carry out supersound extract, the time is 35 minutes, and frequency of oscillation is 30kHz, extracts 3 times, and merge extractive liquid, filters, and filtrate decompression is condensed into extractum;
(2) with the water of 65 ℃ of 33 times of amounts of above-mentioned extractum adding, stir, leave standstill a moment, inclining supernatant, is evaporated to solution: medical material is 1: 1, adds 95% ethanol and makes that containing pure amount is 75%, leaves standstill 24 hours, filters, and precipitates standby;
(3) above-mentioned precipitation is dissolved with 28 times of water gagings, heat when under agitation dripping 1% tannic acid, boil the centrifugal disgorging in back, continue to add tannic acid to solution and do not produce muddiness, cross the polyamide column of having handled well,, collect water lotion with 7 times water elution, it with molecular cut off 32000 ultrafilter membrane ultrafiltration, filtrate reuse molecular cut off is 5600 ultrafilter membrane ultrafiltration, collects not by liquid concentrating under reduced pressure, drying promptly gets polyporusum bellatus;
(4) take by weighing above-mentioned polyporusum bellatus 20g, add lactose, regulating pH value with meglumine is 7.3, and with 0.22 μ m filtering with microporous membrane, lyophilization is packaged to be 1000 of freeze-dried powders of the present invention.
Embodiment 6
(1) get the Polyporus medical material, pulverize, cross 40 mesh sieves, add 9 times of water gagings, soak half an hour, put into the supersound extraction jar, carry out supersound extract, the time is 40 minutes, and frequency of oscillation is 50kHz, extracts 4 times, and merge extractive liquid, filters, and filtrate decompression is condensed into extractum;
(2) with the water of 70 ℃ of 35 times of amounts of above-mentioned extractum adding, stir, leave standstill a moment, inclining supernatant, is evaporated to solution: medical material is 1: 1, adds 95% ethanol and makes that containing pure amount is 82%, leaves standstill 24 hours, filters, and precipitates standby;
(3) above-mentioned precipitation is dissolved with 25 times of water gagings, heat when under agitation dripping 1% tannic acid, boil the centrifugal disgorging in back, continue to add tannic acid to solution and do not produce muddiness, cross the polyamide column of having handled well,, collect water lotion with 6 times water elution, it with molecular cut off 36000 ultrafilter membrane ultrafiltration, filtrate reuse molecular cut off is 6400 ultrafilter membrane ultrafiltration, collects not by liquid concentrating under reduced pressure, drying promptly gets polyporusum bellatus;
(4) take by weighing above-mentioned polyporusum bellatus 20g, add glucose, regulating pH value with meglumine is 8.2, and with 0.22 μ m filtering with microporous membrane, lyophilization is packaged to be 1000 of freeze-dried powders of the present invention.
Claims (2)
1, a kind of polyporusum bellatus lyophilized injectable powder is characterized in that, it is to be prepared from by the polyporusum bellatus of molecular weight 5000-40000 and water-soluble injection pharmaceutic adjuvant.
2, a kind of preparation method of polyporusum bellatus lyophilized injectable powder is characterized in that, it may further comprise the steps:
(1) gets the Polyporus medical material, pulverize, cross 40 mesh sieves, add 8-14 times of water gaging, soak half an hour, put into the supersound extraction jar, carry out supersound extract, the time is 30-60 minute, and frequency of oscillation is 20-80kHz, extract 3-4 time, merge extractive liquid, filters, and filtrate decompression is condensed into extractum;
(2) above-mentioned extractum is added 30-40 and doubly measure 60 ℃-80 ℃ water, stir, leave standstill a moment, inclining supernatant, is evaporated to solution: medical material is 1: 1, adds 95% ethanol and makes that containing pure amount is 70%-90%, leaves standstill 24 hours, filters, and precipitates standby;
(3) above-mentioned precipitation is dissolved with 20-30 times of water gaging, heat when under agitation dripping 1% tannic acid, boil the centrifugal disgorging in back, continue to add tannic acid to solution and do not produce muddiness, cross the polyamide column of having handled well,, collect water lotion with 4-8 water elution doubly, with molecular cut off is the ultrafilter membrane ultrafiltration of 30000-40000, filtrate reuse molecular cut off is the ultrafilter membrane ultrafiltration of 5000-8000, collects not by liquid concentrating under reduced pressure, drying promptly gets polyporusum bellatus;
(4) take by weighing above-mentioned polyporusum bellatus, add the water solublity freeze-dried excipient, regulating pH value with meglumine is 7.0-8.5, and with 0.22 μ m filtering with microporous membrane, lyophilization is packaged to be freeze-dried powder of the present invention.
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| CN 200510008760 CN1682751A (en) | 2005-02-25 | 2005-02-25 | Agaric polysaccharide freeze-dried powder injection and its preparing method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102451163A (en) * | 2010-10-28 | 2012-05-16 | 河北大学 | Polyporus polysaccharide dispersible tablets, and preparation method thereof |
| CN103232553B (en) * | 2013-05-06 | 2015-11-25 | 陕西理工学院 | A kind of semi continuous extracts the apparatus and method of polyporusum bellatus |
-
2005
- 2005-02-25 CN CN 200510008760 patent/CN1682751A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102451163A (en) * | 2010-10-28 | 2012-05-16 | 河北大学 | Polyporus polysaccharide dispersible tablets, and preparation method thereof |
| CN102451163B (en) * | 2010-10-28 | 2013-07-31 | 河北大学 | Polyporus polysaccharide dispersible tablets, and preparation method thereof |
| CN103232553B (en) * | 2013-05-06 | 2015-11-25 | 陕西理工学院 | A kind of semi continuous extracts the apparatus and method of polyporusum bellatus |
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