CN1278698C - Freeze dried injection of seeding of autumnal sowthistle-leaf ixeris and preparation method thereof - Google Patents
Freeze dried injection of seeding of autumnal sowthistle-leaf ixeris and preparation method thereof Download PDFInfo
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- CN1278698C CN1278698C CN 200410083993 CN200410083993A CN1278698C CN 1278698 C CN1278698 C CN 1278698C CN 200410083993 CN200410083993 CN 200410083993 CN 200410083993 A CN200410083993 A CN 200410083993A CN 1278698 C CN1278698 C CN 1278698C
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- herba ixeritis
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- ixeritis sonchifoliae
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Abstract
The present invention discloses a frozen dried injection of sowthistle-leaf ixeris seedling and a preparing method thereof. The frozen dried injection of sowthistle-leaf ixeris seedling is prepared from sowthistle-leaf ixeris seedling extracts and pharmaceutic adjuvants. Sowthistle-leaf ixeris seedling medicinal materials are extracted by an ultrasonic method, extract liquid is treated by high speed centrifugation and ultrafiltration, and polyamide resin poles are used for separation and purification to obtain the sowthistle-leaf ixeris seedling extracts, wherein the contents of total flavonoids which are main effective ingredients are larger than 40%. The results of content measurement and pharmacological experiments show that the injection comprises high contents of effective ingredients and has strong pharmacological actions.
Description
Affiliated technical field
The invention belongs to technical field of traditional Chinese medicine pharmacy, be specifically related to a kind of freeze dried injection of seeding of autumnal sowthistle-leaf ixeris and preparation method thereof.
Background technology
Herba Ixeritis Sonchifoliae has another name called Herba Ixeritis Sonchifoliae, is the living then dry herb of feverfew Herba Ixeritis Sonchifoliae.Nature and flavor hardship, suffering, flat, the tool heat-clearing and toxic substances removing, the effect of reducing swelling and alleviating pain, its main component are flavonoid glycoside composition and ribosidoadenine.Modern study proves that this medicine has effects such as coronary blood flow increasing, the circulation of improvement cardiac muscle to blood circulation, to the remarkable anticoagulant function of blood system energy, obviously increase the activity of fibrinoclase.The preparation that has gone on the market has KUDIEZI ZHUSHEYE (WS-10354 (ZD-0354)-2002), and KUDIEZI ZHUSHEYE has promoting blood circulation and stopping pain, heat clearing away blood stasis dispelling effect, is used for the thoracic obstruction of blood stasis impatency, and card is seen: the thoracic obstruction, pained, bitter taste, tongue is dark red or deposit ecchymosis etc.Be applicable to coronary heart disease, the angina pectoris person that sees the above-mentioned symptom.The person that also can be used for the cerebral infarction.Adopt decocting to boil among the KUDIEZI ZHUSHEYE preparation technology, the removal of impurity of stone mercaptans, this method extract water-solubility impurity is many, the technology more complicated, loss of effective components is big in the preparation process, thereby making the medicine active constituent content low, the injection inorganic salt content height of this method preparation easily produces pain during injection simultaneously.Injection is to the stability requirement height, carry, store, broken easily when transporting, also result in hand cramps.
Patent (application number 02109532) " a kind of treat cardiovascular and cerebrovascular vessel and retinopathy Chinese medicine and preparation method thereof " and patent (application number 03133969) " a kind of Herba Ixeritis Sonchifoliae pharmaceutical preparation and preparation method thereof " a kind of Herba Ixeritis Sonchifoliae injectable powder and preparation method are disclosed respectively, the former is a lyophilized injectable powder, the latter is for to make the powder pin with spray drying method, but these two invented technology method for makings have all been continued to use the preparation method of KUDIEZI ZHUSHEYE (WS-10354 (ZD-0354)-2002), no novelty substantially.
In data-searching, do not find the report of other relevant freeze dried injection of seeding of autumnal sowthistle-leaf ixeris.
Summary of the invention
The objective of the invention is to disclose a kind of active component content height, eutherapeutic Ixers sonchifolia freeze-dried powder preparation.
Another object of the present invention provides the preparation method of above-mentioned freeze-dried powder.
The present invention is achieved through the following technical solutions.
One, preparation method
(1) prescription of crude drug is: Herba Ixeritis Sonchifoliae.
(2) get the Herba Ixeritis Sonchifoliae medical material, pulverized the 20-50 mesh sieve, add 6-8 and doubly measure 70% ethanol, 20KHz frequency supersound extraction secondary, 30min for the first time, 20min for the second time, extracting liquid filtering merges, decompression recycling ethanol, Herba Ixeritis Sonchifoliae extractum.
(3) Herba Ixeritis Sonchifoliae extractum being added an amount of distilled water makes medicinal liquid contain 1g crude drug/ml, stir evenly, cold preservation is placed and is spent the night, and high speed centrifugation is got the supernatant ultrafiltration, polyamide resin column absorption on the ultrafiltrate, wash with water to effluent colourlessly, reuse 3-5 times of column volume amount 60%-80% ethanol elution merges ethanol elution, decompression recycling ethanol also concentrates, and obtains Herba Ixeritis Sonchifoliae extract.
(4) preparation prescription is: Herba Ixeritis Sonchifoliae extract 5-7 weight portion, pharmaceutic adjuvant 40-50 weight portion.
(5) get the said extracted thing and add the dissolving of injection water, filter, add pharmaceutic adjuvant, add the injection water, regulate pH value to 6.0-7.0 to ormal weight, filtering with microporous membrane, fill, lyophilization, promptly.
In the preparation of freeze dried injection of seeding of autumnal sowthistle-leaf ixeris, adopt ultrasonic method to extract effective ingredient, this technology is boiled with traditional decocting, alcohol reflux technology is compared, the extraction temperature is low, extraction time is short, because ultrasonic wave energy is destroyed the cell of vegetable drug, solvent is penetrated in the crude drug cell, thereby effective ingredient is dissolved in the solvent in the acceleration medical material, improves the extraction rate of effective ingredient.High speed centrifugation after the Herba Ixeritis Sonchifoliae extract cold preservation, the supernatant ultrafilter membrane ultrafiltration of molecular cut off 10000-30000, can effectively remove macromole impurity such as the contained matter of mixing of medicinal liquid, protein, resin, starch, polyamide resin column absorption on the ultrafiltrate, wash with water remove partial impurities after, use the ethanol elution desorption, merge eluent, obtain the total flavonoid glycoside constituents.This method can make that main effective ingredient content of total flavone is greater than 40% in the Herba Ixeritis Sonchifoliae extract behind the purification, and adenosine content has improved the content of effective ingredient greater than 0.07%.Medicine exists with solid state after making lyophilized powder, has improved stability of formulation.Pharmacological evaluation proves that lyophilized powder of the present invention has better pharmacological action
The granularity of Herba Ixeritis Sonchifoliae medicinal material coarse powder supersound extraction is preferably the 30-40 mesh sieve among the above preparation technology, and during the polyamide resin column eluting, the concentration of alcohol of eluting polyamide resin column is preferably 70-75%.
Two, assay analysis
1, content of total flavone is measured in the Herba Ixeritis Sonchifoliae preparation
1.1 instrument and reagent: day island proper Tianjin UV-3000A ultraviolet spectrophotometer.KUDIEZI ZHUSHEYE (Tonghua Huaxia Pharmaceutical Co., Ltd., specification 10ml/ props up); Herba Ixeritis Sonchifoliae dry powder injection of the present invention (Tianzhijiao Medication Development Co., Ltd., Guangdong's laboratory provides); Control substance of Rutin (Nat'l Pharmaceutical ﹠ Biological Products Control Institute); Ethanol, sodium nitrite, aluminum nitrate, sodium hydroxide reagent are analytical pure, and water is tri-distilled water (self-control).
1.2 experimental technique: the assay method with reference to KUDIEZI ZHUSHEYE (WS-10354 (ZD-0354)-2002) total flavones is measured, and the results are shown in Table 1.
Total flavones in table 1 preparation (in anhydrous rutin) assay
| Lot number | Content (mg/ props up) | |
| KUDIEZI ZHUSHEYE | Freeze dried injection of seeding of autumnal sowthistle-leaf ixeris of the present invention | |
| 1 2 3 | 7.2 6.9 7.5 | 24.3 23.8 22.1 |
2, the assay of adenosine in the Herba Ixeritis Sonchifoliae preparation
Content assaying method with reference to adenosine in KUDIEZI ZHUSHEYE (WS-10354 (ZD-0354)-2002) quality standard is measured, and the results are shown in Table 2.
The assay of adenosine in table 2 preparation
| Lot number | Content (mg/ props up) | |
| KUDIEZI ZHUSHEYE | Freeze dried injection of seeding of autumnal sowthistle-leaf ixeris of the present invention | |
| 1 2 3 | 0.031 0.032 0.029 | 0.045 0.043 0.042 |
Above assay presentation of results, total flavones in the freeze dried injection of seeding of autumnal sowthistle-leaf ixeris of the present invention and adenosine content are apparently higher than total flavones and the adenosine content of KUDIEZI ZHUSHEYE (WS-10354 (ZD-0354)-2002).Illustrate that preparation method of the present invention is more reasonable, science has more practicality more.
3, the assay of total flavones and adenosine in the extract
Determining instrument, method are the same.Extract 1 (extracting method by KUDIEZI ZHUSHEYE (WS-10354 (ZD-0354)-2002) makes); Extract 2 (by Herba Ixeritis Sonchifoliae lyophilized powder extract process preparation of the present invention); The results are shown in Table 3 and table 4.
Total flavones in table 3 extract (in anhydrous rutin) assay
| Batch | Content (%) | |
| Extract 1 | Extract 2 | |
| 1 2 3 | 10.2 11.0 10.5 | 42.1 42.5 41.7 |
Adenosine content is measured in table 4 extract
| Batch | Content (%) | |
| Extract 1 | Extract 2 | |
| 1 2 3 | 0.044 0.046 0.041 | 0.075 0.072 0.070 |
Above assay presentation of results, effective ingredient total flavones and adenosine content total flavones and the adenosine content in the KUDIEZI ZHUSHEYE extract (according to the method for making gained in (WS-10354 (ZD-0354)-2002)) in the extract that preparation method of the present invention obtains.Preparation method of the present invention science more rationally is described, has more practicality.
Four, pharmacology embodiment
Reagent; Normal saline (Tianzhijiao Medication Development Co., Ltd., Guangdong's laboratory provides)
KUDIEZI ZHUSHEYE (Tonghua Huaxia Pharmaceutical Co., Ltd.)
Freeze dried injection of seeding of autumnal sowthistle-leaf ixeris of the present invention (Tianzhijiao Medication Development Co., Ltd., Guangdong's laboratory provides).
1, to the influence of coronary flow
Animal: healthy hybrid dog, 18, male and female dual-purpose, body weight 12-15kg.
Experimental technique: 18 dogs are divided into 3 groups at random, every group 6, normal saline group dosage is 2ml/kg, and administration group dosage is 1.5g/kg, and dog is anaesthetized with 3% pentobarbital sodium (30mg/kg), right arm reclining is fixed on the operating-table, separate femoral vein, insert venous cannulation, slowly import normal saline through vein, in order to administration, separate trachea, insert tracheal intubation and connect the capable malleation artificial respiration of respirator, 22 times/min of frequency, the 4-5 intercostal is opened the thoracic cavity in the left side, expose heart, separate ramus descendens anterior arteriae coronariae sinistrae top, place electromagnetic flowmeter probe (internal diameter 2mm-3mm), measure the coronary flow of whenever fighting, experiment finishes to take out heart and weighs.After operation finished, animal was stablized 20min, measured the coronary flow of whenever fighting, and 30min after the administration measures the coronary flow of whenever fighting again, calculates coronary flow and rate of change by following formula:
Coronary flow (ml/min100g)=whenever coronary flow (ml) * heart rate/cardiac weight (g) * 100 of fighting
Before rate of change=(after the administration-administration before)/administration * 100%
Experimental result sees Table 4.
The influence of table 4 pair dog coronary flow
| Grouping | Coronary flow (ml/min100g) | ||
| Before the administration | After the administration | Rate of change (%) | |
| The normal saline Herba Ixeritis Sonchifoliae is penetrated liquid Herba Ixeritis Sonchifoliae lyophilized powder of the present invention | 51.4±9.8 47.5±10.9 * 50.1±9.2 *# | 51.5±11.3 50.9±15.4 * 55.2±9.9 *# | 0.3±4.8 7.8±6.5 * 9.5±7.0 *# |
With normal saline group ratio
*P＜0.01 and injection ratio
#P＜0.05
Experimental result shows: freeze dried injection of seeding of autumnal sowthistle-leaf ixeris of the present invention and KUDIEZI ZHUSHEYE be the remarkable coronary blood flow increasing of energy all, but the effect of freeze dried injection of seeding of autumnal sowthistle-leaf ixeris of the present invention is obviously strong than KUDIEZI ZHUSHEYE, illustrates: the pharmacological action of freeze dried injection of seeding of autumnal sowthistle-leaf ixeris of the present invention is better than KUDIEZI ZHUSHEYE.
2, to hemorheological influence
Animal: Wistar rat, 40, male and female half and half, body weight 250 ± 5g.
Experimental technique: get the Wistar rat, be divided into 4 groups at random, every group 10, normal saline group and matched group intraperitoneal injection of saline every day 10ml/kg, continuous 7 days, the 7th day subcutaneous injection 0.1% epinephrine 0.8mg/kg of model group, after 2 hours rat immersed in the frozen water 5 minutes, injecting epinephrine in 2 hours again stops eating after once, administration group successive administration 7 days, administration group dosage is 5g/kg, and the modeling broken end was got blood and measured hemorheology with the multi-functional blood viscosity instrument of 3-9 type computer as stated above in the 7th day, the results are shown in Table 5.
The hemorheological influence of table 5 pair rat " syndrome of blood stasis "
| Grouping | Coronary flow (ml/min.100g) | |||
| Whole blood contrast viscosity (height is cut) | Plasma viscosity | Relative viscosity | Erythrocyte aggregation index | |
| Normal saline model group KUDIEZI ZHUSHEYE Herba Ixeritis Sonchifoliae lyophilized powder of the present invention | 3.22±1.2 6.12±1.5* 4.05±1.4 # 3.75±1.0 #Δ | 1.25±0.9 2.95±1.3 * 1.75±1.0 # 1.42±1.1 #Δ | 2.85±1.0 5.0±1.3 * 3.78±1.2 # 3.09±1.4 #Δ | 18.52±1.9 27.2±2.2 * 21.3±2.0 # 19.0±1.8 #Δ |
With normal saline group ratio
*P＜0.01 is with model group group ratio
#P＜0.01 is with the KUDIEZI ZHUSHEYE ratio
ΔP＜0.05
The pharmacological results shows: KUDIEZI ZHUSHEYE and Herba Ixeritis Sonchifoliae lyophilized powder of the present invention are to the effect that all has clear improvement of " syndrome of blood stasis " hemorheology of rat, the proof Herba Ixeritis Sonchifoliae has definite blood circulation promoting and blood stasis dispelling, the effect of microcirculation improvement, Herba Ixeritis Sonchifoliae lyophilized powder wherein of the present invention has than the better pharmacological action of KUDIEZI ZHUSHEYE.
Six, preparation embodiment,
Embodiment 1:
(1) prescription of crude drug is: Herba Ixeritis Sonchifoliae 1000g.
(2) get the Herba Ixeritis Sonchifoliae medical material, pulverized 40 mesh sieves, add 7 times of amount 70% ethanol, 20KHz frequency supersound extraction secondary, 30min for the first time, 20min for the second time, extracting liquid filtering merges, decompression recycling ethanol, Herba Ixeritis Sonchifoliae extractum.
(3) Herba Ixeritis Sonchifoliae extractum being added an amount of distilled water makes medicinal liquid contain 1g crude drug/ml, stir evenly, cold preservation is placed and is spent the night, and high speed centrifugation is got the supernatant ultrafiltration, polyamide resin column absorption on the ultrafiltrate, wash with water to effluent colourlessly, 4 times of column volume amount 70% ethanol elutions of reuse merge 70% ethanol elution, decompression recycling ethanol also concentrates, and obtains Herba Ixeritis Sonchifoliae extract 6.0g.
(4) preparation prescription is: Herba Ixeritis Sonchifoliae extract 6.0g, mannitol 44g.、
(5) get the said extracted thing and add the dissolving of injection water, filter, add mannitol, add the injection water, regulate pH value to 6.0-7.0 to ormal weight, filtering with microporous membrane, fill, lyophilization, promptly.
Embodiment 2:
(1) prescription of crude drug is: Herba Ixeritis Sonchifoliae 1000g.
(2) get the Herba Ixeritis Sonchifoliae medical material, pulverized 30 mesh sieves, add 8 times of amount 70% ethanol, 20KHz frequency supersound extraction secondary, 30min for the first time, 20min for the second time, extracting liquid filtering merges, decompression recycling ethanol, Herba Ixeritis Sonchifoliae extractum.
(3) Herba Ixeritis Sonchifoliae extractum being added an amount of distilled water makes medicinal liquid contain 1g crude drug/ml, stir evenly, cold preservation is placed and is spent the night, and high speed centrifugation is got the supernatant ultrafiltration, polyamide resin column absorption on the ultrafiltrate, wash with water to effluent colourlessly, 5 times of column volume amount 75% ethanol elutions of reuse merge 75% ethanol elution, decompression recycling ethanol also concentrates, and obtains Herba Ixeritis Sonchifoliae extract 6.5g.
(4) preparation prescription: Herba Ixeritis Sonchifoliae extract 6.5g, glucose 25g, mannitol 20g.
(5) get the said extracted thing and add the dissolving of injection water, filter, add glucose, mannitol, add the injection water, regulate pH value to 6.0-7.0 to ormal weight, filtering with microporous membrane, fill, lyophilization, promptly.
Embodiment 3:
(1) prescription of crude drug is: Herba Ixeritis Sonchifoliae 800g.
(2) get the Herba Ixeritis Sonchifoliae medical material, pulverized 50 mesh sieves, add 6 times of amount 70% ethanol, 20KHz frequency supersound extraction secondary, 30min for the first time, 20min for the second time, extracting liquid filtering merges, decompression recycling ethanol, Herba Ixeritis Sonchifoliae extractum.
(3) Herba Ixeritis Sonchifoliae extractum being added an amount of distilled water makes medicinal liquid contain 1g crude drug/ml, stir evenly, cold preservation is placed and is spent the night, and high speed centrifugation is got the supernatant ultrafiltration, polyamide resin column absorption on the ultrafiltrate, wash with water to effluent colourlessly, 3 times of column volume amount 60% ethanol elutions of reuse merge ethanol elution, decompression recycling ethanol also concentrates, and obtains Herba Ixeritis Sonchifoliae extract 5.0g.
(4) preparation prescription: Herba Ixeritis Sonchifoliae extract 5.0g, Dextran 45 g.
(5) get the said extracted thing and add the dissolving of injection water, filter, add glucosan, add the injection water, regulate pH value to 6.0-7.0 to ormal weight, filtering with microporous membrane, fill, lyophilization, promptly.
Embodiment 4:
(1) prescription of crude drug is: Herba Ixeritis Sonchifoliae 1200g.
(2) get the Herba Ixeritis Sonchifoliae medical material, pulverized 20 mesh sieves, add 8 times of amount 70% ethanol, 20KHz frequency supersound extraction secondary, 30min for the first time, 20min for the second time, extracting liquid filtering merges, decompression recycling ethanol, Herba Ixeritis Sonchifoliae extractum.
(3) Herba Ixeritis Sonchifoliae extractum being added an amount of distilled water makes medicinal liquid contain 1g crude drug/ml, stir evenly, cold preservation is placed and is spent the night, and high speed centrifugation is got the supernatant ultrafiltration, polyamide resin column absorption on the ultrafiltrate, wash with water to effluent colourlessly, 5 times of column volume amount 60% ethanol elutions of reuse merge ethanol elution, decompression recycling ethanol also concentrates, and obtains Herba Ixeritis Sonchifoliae extract 7.0g.
(4) preparation prescription: Herba Ixeritis Sonchifoliae extract 7.0g, dextrorotation fructose 40g, lactose 10g.
(5) get the said extracted thing and add the dissolving of injection water, filter, add pharmaceutic adjuvant, add the injection water, regulate pH value to 6.0-7.0 to ormal weight, filtering with microporous membrane, fill, lyophilization, promptly.
Embodiment 5:
(1) prescription of crude drug is: Herba Ixeritis Sonchifoliae 900g.
(2) get the Herba Ixeritis Sonchifoliae medical material, pulverized 30 mesh sieves, add 6 times of amount 70% ethanol, 20KHz frequency supersound extraction secondary, 30min for the first time, 20min for the second time, extracting liquid filtering merges, decompression recycling ethanol, Herba Ixeritis Sonchifoliae extractum.
(3) Herba Ixeritis Sonchifoliae extractum being added an amount of distilled water makes medicinal liquid contain 1g crude drug/ml, stir evenly, cold preservation is placed and is spent the night, and high speed centrifugation is got the supernatant ultrafiltration, polyamide resin column absorption on the ultrafiltrate, wash with water to effluent colourlessly, 4 times of column volume amount 75% ethanol elutions of reuse merge ethanol elution, decompression recycling ethanol also concentrates, and obtains Herba Ixeritis Sonchifoliae extract 5.5g.
(4) preparation prescription: Herba Ixeritis Sonchifoliae extract 5.5g, mannitol 35g, dextrorotation fructose 10g.
(5) get the said extracted thing and add the dissolving of injection water, filter, add pharmaceutic adjuvant, add the injection water, regulate pH value to 6.0-7.0 to ormal weight, filtering with microporous membrane, fill, lyophilization, promptly.
Embodiment 6:
(1) prescription of crude drug is: Herba Ixeritis Sonchifoliae 1100g.
(2) get the Herba Ixeritis Sonchifoliae medical material, pulverized 40 mesh sieves, add 8 times of amount 70% ethanol, 20KHz frequency supersound extraction secondary, 30min for the first time, 20min for the second time, extracting liquid filtering merges, decompression recycling ethanol, Herba Ixeritis Sonchifoliae extractum.
(3) Herba Ixeritis Sonchifoliae extractum being added an amount of distilled water makes medicinal liquid contain 1g crude drug/ml, stir evenly, cold preservation is placed and is spent the night, and high speed centrifugation is got the supernatant ultrafiltration, polyamide resin column absorption on the ultrafiltrate, wash with water to effluent colourlessly, 3 times of column volume amount 80% ethanol elutions of reuse merge ethanol elution, decompression recycling ethanol also concentrates, and obtains Herba Ixeritis Sonchifoliae extract 6.4g.
(4) preparation prescription is: Herba Ixeritis Sonchifoliae extract 6.4g, mannitol 28g, lactose 15g.
(5) get the said extracted thing and add the dissolving of injection water, filter, add pharmaceutic adjuvant, add the injection water, regulate pH value to 6.0-7.0 to ormal weight, filtering with microporous membrane, fill, lyophilization, promptly.
Claims (5)
1, a kind of freeze dried injection of seeding of autumnal sowthistle-leaf ixeris, it is characterized in that it is to be made by Herba Ixeritis Sonchifoliae extract 5-7 weight portion and pharmaceutic adjuvant 40-50 weight portion, wherein content of total flavone is more than or equal to 40% in the Herba Ixeritis Sonchifoliae extract, and the extracting method of described Herba Ixeritis Sonchifoliae extract is:
(1) get the Herba Ixeritis Sonchifoliae medical material, pulverized the 20-50 mesh sieve, add 6-8 and doubly measure 70% ethanol, 20KHz frequency supersound extraction secondary, 30min for the first time, 20min for the second time, extracting liquid filtering merges, decompression recycling ethanol, Herba Ixeritis Sonchifoliae extractum;
(2) Herba Ixeritis Sonchifoliae extractum being added an amount of distilled water makes every milliliter of medicinal liquid contain the 1g crude drug, stir evenly, cold preservation is placed and is spent the night, and high speed centrifugation is got the supernatant ultrafiltration, polyamide resin column absorption on the ultrafiltrate, wash with water to effluent colourlessly, reuse 3-5 times of column volume amount 60%-80% ethanol elution merges ethanol elution, decompression recycling ethanol also concentrates, and obtains Herba Ixeritis Sonchifoliae extract.
2, freeze dried injection of seeding of autumnal sowthistle-leaf ixeris according to claim 1, wherein pharmaceutic adjuvant is one or both the mixture in mannitol, glucosan, glucose, fructose, dextrorotation fructose and the lactose.
3, freeze dried injection of seeding of autumnal sowthistle-leaf ixeris according to claim 1, wherein the Herba Ixeritis Sonchifoliae pulverizing medicinal materials becomes 30-40 order coarse powder.
4, freeze dried injection of seeding of autumnal sowthistle-leaf ixeris according to claim 1, wherein the concentration of alcohol of eluting polyamide resin column is 70%-75%.
5, freeze dried injection of seeding of autumnal sowthistle-leaf ixeris according to claim 1, its preparation method is: get Herba Ixeritis Sonchifoliae extract and add the dissolving of injection water, filter, add pharmaceutic adjuvant, add the injection water to ormal weight, regulate pH value to 6.0-7.0, filtering with microporous membrane, fill, lyophilization, promptly.
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| CN 200410083993 CN1278698C (en) | 2004-10-15 | 2004-10-15 | Freeze dried injection of seeding of autumnal sowthistle-leaf ixeris and preparation method thereof |
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| CN108420842A (en) * | 2018-04-28 | 2018-08-21 | 通化师范学院 | A kind of sowthistle-leaf ixeris seedling preparation of antigout effect |
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