CN103228787B - 用于分泌性蛋白表达的融合蛋白 - Google Patents

用于分泌性蛋白表达的融合蛋白 Download PDF

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CN103228787B
CN103228787B CN201180046204.3A CN201180046204A CN103228787B CN 103228787 B CN103228787 B CN 103228787B CN 201180046204 A CN201180046204 A CN 201180046204A CN 103228787 B CN103228787 B CN 103228787B
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约恩·路易林克
沃特·S·P·钟
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Abstract

提供了一种适合于多于一种感兴趣多肽（POI）的分泌的融合蛋白，其包括信号肽、POI、包含来自自转运蛋白的β茎部结构域的载客结构域、以及来自自转运蛋白的易位蛋白结构域，其中该载客结构域的β茎部形成序列是基本上完整的并且该（这些）POI与该β茎部结构域融合。

Description

用于分泌性蛋白表达的融合蛋白
技术领域
本发明总体上涉及一种新的融合蛋白和一种用于分泌性蛋白表达的方法。
背景技术
分泌性蛋白表达是宿主细胞中蛋白质的表达，其中该蛋白质输出至细胞膜并且以可溶性方式释放至培养基中或保持与细胞膜连接。分泌性蛋白表达由处在蛋白质N端的信号肽介导，该信号肽指导多肽到达细胞膜。
通常，在原核宿主如大肠杆菌中产生的重组蛋白以胞内方式产生。当蛋白质以这种方式回收时，不得不裂解细胞，这导致重组蛋白被细胞内容物污染。随后不得不从全细胞提取物中以多步骤纯化流程回收蛋白质，这费时并且导致低劣产率。
将重组蛋白分泌至培养基中是一项更好的策略，因为从用过的培养基中纯化蛋白质较容易并且与连续培养更相容。然而，现有系统不具有高效的产率。
其中蛋白质保持与细胞表面连接的分泌性蛋白表达具有其他用途。这种类型的蛋白质表达的用途的实例包括活疫苗开发、表位定位、生物吸附剂和生物传感器开发和为发现药物而高通量筛选蛋白质和肽文库。
在表面展示和分泌中，重组蛋白面临跨过复杂大肠杆菌细胞包膜易位的挑战，该细胞包膜由两个脂质膜(内膜和外膜)组成，在其间存在凝胶样区室(周质)。已经显示这种易位是十分困难的并且先前使用的方法具有低效力。
自转运蛋白是巨大的蛋白质，它们由革兰氏阴性细菌如(大肠杆菌)分泌。自转运蛋白系统在以下意义上是简单的：认为自转运蛋白，如其名称暗示，在蛋白质自身内部携带用于跨周质和外膜易位的全部信息。然而，借以自转运蛋白分泌的机制仍是不完全理解的。
自转运蛋白合成为巨大的前体蛋白，该前体蛋白含有3个主要结构域：(i)将蛋白质靶向Sec易位子并启动跨内膜转移的N端信号肽，(ii)包含待分泌的“载货”蛋白的载客结构域和(iii)包含β桶结构的C端成孔结构域(易位蛋白结构域)，其中该β桶结构整合至外膜中并且在载客结构域跨越外膜易位至胞外空间中发挥关键但不清楚的作用。
在易位后，载客结构域从易位蛋白结构域中被切下并释放至胞外环境中。在一些情况下，载客结构域保持与细胞表面非共价地连接。可以通过(外部)蛋白酶对位于易位蛋白结构域和载客结构域之间的蛋白酶基序发挥作用来实现切割。可替代地，切割通过易位蛋白结构域和载客结构域之间特定位点处的分子内自催化事件发生。
自转运蛋白的载客结构域包含β茎部结构和侧面结构域。β茎部是由延长的β螺旋形成的伸长结构。载客结构域的C端包含已经参与载客结构域折叠和跨外膜易位的自伴侣结构域。
Hbp是属于肠杆菌科(Enterobacteriaceae)丝氨酸蛋白酶自转运蛋白(SPATE)亚家族的自转运蛋白。Hbp的载客结构域的晶体结构最近已经测定(Otto等人，2005,J.BiolChem(《生物化学杂志》)280(17)：17339-45)，并且如图11A显示。该结构展示这种多肽形成长的右手β-螺旋结构(“β茎部”)。Hbp的载客结构域包含两个较大的侧面结构域：结构域d1和结构域d2，其中d1包含该蛋白质的丝氨酸蛋白酶活并且d2具有未知的功能。还存在3个较小的侧面结构域：结构域3(d3)、结构域4(d4)和结构域5(d5)。
还已经显示了其他自转运蛋白如百日咳杆菌粘着素(Emsley等人，1996Nature(《自然》)381：90-92)和IgA蛋白酶(Johnson等人，2009J Mol Biol(《分子生物学杂志》)389(3)：559-74)的相似β茎部结 构域。
先前已经尝试使用自转运蛋白用于大肠杆菌中的分泌性蛋白表达，大多使用经工程化用于表面展示目的的奈瑟氏菌IgA蛋白酶(Pyo等人，2009Vaccine(《疫苗》)27 2030-2036)和内源性大肠杆菌自转运蛋白AIDA-I(Van Gerven等人，2009Microbiology(《微生物学》)155:468-476)的变体。
使用IgA蛋白酶和AIDA-I用于分泌重组蛋白的尝试使用了导致分泌和表面暴露的蛋白质的低劣产率的构建体(Pyo等人，2009Vaccine(《疫苗》)27 2030-2036；Van Gerven等人，2009Microbiology(《微生物学》)155:468-476)。在大多数的这类研究中，完整或几乎完整的内源性载客结构域由重组蛋白替换。
迄今，自转运蛋白已经主要用作展示平台，而非用于可溶性形式的异源蛋白质的分泌，其中该蛋白质被分泌至培养基中。
IgA蛋白酶需要附属蛋白酶用于加工，而AIDA-I在切割后保持与外膜非共价地连接。因此，这些自转运蛋白可能仅用于表位和蛋白质的表面呈递。
先前已经显示了与Hbp载客结构域融合的钙调蛋白的高效展示和分泌(Jong等人，2007Molecular Microbiology(《分子微生物学》)63:1524-1536)。为了最小化对载客结构域的天然β-茎部的扰动，钙调蛋白替换Hbp载客结构域的结构域2。
对于某些应用，从细胞表面分泌或展示多于一种感兴趣蛋白(POI)的可能性是十分有用的。这类应用包括疫苗，例如其中两个或更多个表位展示在相同的细胞表面上；酶展示，其中多于一个酶展示在细胞表面上来以一系列步骤实施众多催化反应；肽文库暴露和抑制剂筛选。
对于多价疫苗，特别有用的是具有一种系统，其中一个宿主细胞群体可以表达并展示或分泌多种抗原，而非具有细胞群体的混合物，每个群体仅展示或分泌这些抗原之一。仅具有展示或分泌多种抗原的一个细胞群体具有更易生产和更好控制疫苗含量的优点。
总之，需要改进的分泌表达系统，这些系统用于展示异源蛋白质以及将异源蛋白质以可溶性形式分泌至培养基中。也需要的一种系统和一种方法，其能够在宿主细胞的细胞表面上使多于一个感兴趣蛋白进行分泌性蛋白表达或分泌多于一种蛋白质至培养基中。
发明目的
本发明的一个目的是提供感兴趣多肽(POI)从宿主细胞中的高效分泌。
本发明的第二目的是提供POI在宿主细胞表面上的高效展示。
本发明的第三目的是提供POI向其中培养宿主细胞的培养基中的高效可溶性分泌。
本发明的又一个目的是提供用于多于一种POI的高效分泌(即展示或可溶性分泌)的支架。
发明简述
在本发明的第一方面，提供了能够表达多于一种(如至少两种)POI(感兴趣蛋白)的宿主细胞。这些POI包含于一种融合蛋白中，该融合蛋白还包含载客结构域(其包含来自自转运蛋白的β茎部结构域)、来自自转运蛋白的易位蛋白结构域和能够将该融合蛋白靶向革兰氏阴性细菌内膜的信号肽。该载客结构域的β茎部形成序列是基本上完整的并且这些POI与该载客结构域融合。
与其他系统相比，用于分泌性蛋白表达的这种宿主细胞具有几个优点，包括但不限于更高效地分泌多于一种POI。另外，当目标是展示POI时，随着POI将进一步离开细胞表面并且更稳定，β茎部结构域将能够实现更高效的展示。
在本发明的宿主细胞的一个实施例中，自转运蛋白的天然形式的载客结构域包含从β茎部结构域突出的至少一个侧面结构域。POI随后可 以插入、替代或部分替代这种侧面结构域。
在另一个实施例中，自转运蛋白的天然形式的载客结构域包含至少两个侧面结构域。每种POI随后可以插入、替代或部分替代独立的这种侧面结构域，或POI可以插入、替代或部分替代相同的侧面结构域。
POI也可以与来自自转运蛋白的独立的载客结构域、易位蛋白结构域和信号肽融合。
在本发明的第二方面，提供一种融合蛋白，该融合蛋白包含多于一种(如至少两种)POI(感兴趣蛋白)、载客结构域(其包含来自自转运蛋白的β茎部结构域)、来自自转运蛋白的易位蛋白结构域和任选地，将该融合蛋白靶向革兰氏阴性细菌内膜的信号肽。该载客结构域的β茎部形成序列是基本上完整的并且这些POI与该载客结构域融合。
在其天然形式下，该融合蛋白的载客结构域可以包含从β茎部结构域突出的至少一个侧面结构域，并且这些POI可以插入、替代或部分替代这种侧面结构域。在其天然形式下，该融合蛋白的载客结构域还可以包含至少两个侧面结构域，并且每种POI可以插入、替代或部分替代这类侧面结构域的独立结构域。可替代地，这些POI可以插入、替代或部分替代相同的侧面结构域。
在本发明的另一个方面，提供了经安排用于表达融合蛋白的核酸。在一个实施例中，该核酸在框内包含编码该融合蛋白的信号肽的序列，该信号肽能够将该融合蛋白靶向革兰氏阴性细菌的内膜；编码该融合蛋白的载客结构域的序列，该载客结构域包含来自自转运蛋白的β茎部结构域，和编码该融合蛋白的易位蛋白结构域的序列，该易位蛋白结构域源自自转运蛋白。编码该载客结构域的序列包含允许框内地克隆至少两个编码POI(感兴趣蛋白)的DNA序列的至少两段克隆位点序列。这些克隆位点序列如此安排，从而该载客结构域的所编码的β茎部形成蛋白序列是基本上完整的。也可能不使用克隆位点仅通过融合两段DNA，例如通过PCR，将POI插入一种自转运蛋白中，从而产生一种融合蛋白。
编码该自转运蛋白的载客结构域的序列可以在其天然形式下包含 至少两段序列，这些序列编码从β茎部结构域突出的侧面结构域。该至少两段克隆位点序列随后可以插入、替代或部分替代独立的编码侧面结构域的这类片段。
在另一个实施例中，该核酸在框内包含编码该融合蛋白的信号肽的序列，该信号肽能够将该融合蛋白靶向革兰氏阴性细菌的内膜；编码该融合蛋白的载客结构域的序列，该载客结构域包含来自自转运蛋白的β茎部结构域；编码该融合蛋白的易位蛋白结构域的序列，该易位蛋白结构域源自自转运蛋白；和编码该融合蛋白的至少两种POI的序列。编码这些POI的序列与编码该载客结构域的序列融合并且如此安排，从而该载客结构域的所编码的β茎部形成蛋白序列是基本上完整的。
编码该自转运蛋白的载客结构域的序列在其天然形式下可以包含至少两段序列，这些序列编码从β茎部结构域突出的侧面结构域。编码POI的至少两个序列的每一个随后可以插入、替代或部分替代编码侧面结构域的这些片段的每一个。
可以如此安排该宿主细胞、融合蛋白或核酸，从而当表达时，该融合蛋白从细胞表面分泌。例如，该融合蛋白可以包含使得该融合蛋白被切割和从表达该融合蛋白的宿主细胞分泌的切割位点。并且，编码该融合蛋白的核酸可以编码这种切割位点。
可以如此安排该宿主细胞、融合蛋白或核酸，从而当表达时，该融合蛋白在细胞表面展示。例如，该融合蛋白可以不包含这类切割位点或可以包含破坏的切割位点。类似地，编码该融合蛋白的核酸则不编码这种切割位点或编码破坏的切割位点。可替代地，该融合蛋白和核酸可以包含切割位点并且所得到的融合蛋白经切割，但是保持与细胞表面非共价连接，并且因此在该细胞表面展示。
该载客结构域和易位蛋白结构域可以源自SPATE(肠杆菌科丝氨酸蛋白酶自转运蛋白)蛋白，如来自大肠杆菌的血红蛋白结合蛋白酶(Hbp)、胞外丝氨酸蛋白酶(EspC)或温度敏感性血凝素(Tsh)。
在本发明的一个方面，提供了一种包含本发明核酸的载体。
在本发明的另一个方面，提供了一种包含本发明核酸或载体的宿主细胞。
在一个实施例中，本发明的宿主细胞是革兰氏阴性细菌，该革兰氏阴性细菌可以选自肠杆菌科，如大肠杆菌、沙门氏菌属物种(Salmonella spp.)、弧菌属物种(Vibriospp.)、志贺氏菌属物种(Shigella spp.)、假单胞菌属物种(Pseudomonads spp.)、伯克霍尔德菌属物种(Burkholderia spp.)或德特氏菌属物种(Bordetella spp.)。
在一个方面，提供了展示根据本发明的融合蛋白的外膜小泡。在另一个方面，提供了一种展示根据本发明的融合蛋白的菌影。
在一个方面，提供了一种用于融合蛋白的分泌性蛋白表达的方法，该方法包括步骤：提供根据本发明的一种宿主细胞并诱导该融合蛋白的表达。
在一个实施例中，该方法包括另外的步骤：抑制该宿主细胞中具有蛋白酶活性的周质酶，如DegP。可以例如通过DegP催化位点中的突变抑制DegP。
在另一个实施例中，该方法包括另外的步骤：下调该宿主细胞的周质间隙内催化蛋白质中二硫键形成的至少一种酶，如DsbA或DsbB。
该方法能以可溶性方式提供该融合蛋白的分泌。可替代地，它可以提供该融合蛋白在细胞表面上的展示。
在一个实施例中，该方法包括另外的步骤：诱导小泡从该宿主细胞(其是革兰氏阴性细菌)的外膜排出，因此形成在它们的表面上展示该融合蛋白的外膜小泡。
在另一个实施例中，该方法包括另外的步骤：裂解该革兰氏阴性细菌，因此形成在它们的表面上展示该融合蛋白的菌影。可以通过使用来自噬菌体PhiX174的致死性裂解基因E进行裂解。
在一个实施例中，这些POI的至少一种可以包含抗原，例如来自感染性生物的抗原。该抗原例如是来自结核分枝杆菌(Mycobacterium tuberculosis)的抗原，如ESAT-6、Ag85B、Rv2660c、TB10.4和TB10.3，或与这些蛋白质相似的蛋白质。
在一个方面，提供了包含根据本发明的宿主细胞、融合蛋白、外膜小泡或菌影的疫苗。
附图简述
本发明现在通过举例方式参考附图进行描述，在这些附图中：
图1-10显示在实例中所用的质粒的质粒图。
图11A-D显示Hbp载客结构域的结构的图，其中指出某些结构域。
图11E展示在实例1-15中使用的融合蛋白的多种构建体。
图12-33显示来自实例1-19的实验数据。对于细节，参见实例部分。
图34显示在实例中所用的质粒的质粒图。
定义
如本文所用，供应以下定义旨在促进对本发明的理解。
“自转运蛋白”是属于pfam自转运蛋白家族(‘自转运蛋白’PF03797)并且还已知或预测形成β茎部基序的蛋白质。用于序列分析的BETAWRAPPRO方法可以用来预测自转运蛋白的载客结构域是否将形成β茎部基序(Junker等人，2006Proc Natl Acad Sci U S A(《美国科学院院刊》)103(13)：4918-23)。
“感兴趣多肽”(POI)是本发明的使用者想要宿主细胞以可溶性形式分泌至培养基中或展示在细胞表面上或两者的多肽。典型地，该POI是使用者研究或想要表达的蛋白质，而融合蛋白的其他部分辅助分泌过程。典型地，该POI还相对于与其融合的自转运蛋白结构域是异源的。该POI具有至少4个氨基酸长度、至少10个氨基酸长度或至少20个氨基酸 长度。
“β茎部形成序列”指形成β茎部结构的自转运蛋白的载客结构域的序列。可以使用晶体结构测定法鉴定载客结构域的β茎部形成序列。如上文所述，可以可替代地使用M4T同源性建模方法(Rykunov等人，2009J Struct Funct Genomics(《结构与功能基因组学杂志》)10：95-99)或相似的预测方法鉴定β茎部形成序列。
“侧面结构域”是一种结构域，它是载客结构域的部分但不是β茎部的部分。典型地，侧面结构域位于载客结构域中两段β茎部形成序列之间。侧面结构域始于居前β链之后的第一氨基酸处并且它止于后随于侧面结构域的β链的起始氨基酸之前的一个氨基酸。侧面结构域也可以位于载客结构域的N端处。自转运蛋白可以具有几个侧面结构域。
“相似的蛋白质”、“相似的序列”或“类似的蛋白质”指当两个氨基酸序列比较时，与另一种蛋白质具有高程度同源性的蛋白质。优选地，当两个序列最佳比对时，它至少80％、更优选地多于90％、更优选地多于95％、甚至更优选地多于97％与对比序列同源。使用公众可获得的软件如BLAST，可以轻易地测量序列同源性。
“宿主细胞”指已经向其中导入本发明的一个或多个载体或分离和纯化的核酸序列的原核细胞。应当理解本术语不仅指具体的对象细胞，还指这种细胞的子代或潜在子代。因为某些修饰可以因突变或环境影响而出现在后续世代中，所以这类子代实际上可以与亲代细胞不相同，但仍包含于如本文中所用的这个术语范围内。
“展示”：分泌性蛋白保持与宿主细胞外膜结合，从而它至少部分地在细胞外部突出时，这种分泌性蛋白展示在分泌性宿主细胞的表面上。该分泌性蛋白可以与细胞膜或存在于其中的组分(如来自自转运蛋白的易位蛋白结构域)以共价或非共价的方式连接。
“可溶性分泌”和“以可溶性方式分泌”指蛋白质的分泌，其中该蛋白质分泌入胞外空间中，从而不与宿主细胞结合，这与该蛋白质保持与宿主细胞外膜或整合至宿主细胞外膜中的蛋白质结合时相反。
“大约”指示在适用的情况下，偏离该值+/-10％。
描述
发明人已经发现，如果自转运蛋白的载客结构域的β茎部形成序列是基本上完整的，则该自转运蛋白可以用于改进的分泌性蛋白表达。尽管实际的β茎部形成序列是最佳分泌必需的，但是自转运蛋白的载客结构域的侧面结构域是用于插入POI的合适位点。这些侧面结构域可以由随后将被分泌的POI替换。可替代地，可以插入该POI，从而以便替代部分的侧面结构域或从而以便与侧面结构域融合。
发明人还已经发现，如果自转运蛋白的载客结构域的β茎部形成序列是基本上完整的，则该自转运蛋白可以用于多于一种(如至少两种)POI的改进的分泌性蛋白表达。通过将POI融合，即插入、替换或部分地替换载客结构域的一个或多个侧面结构域，同时保持β茎部结构完整，实现了一种高效和相对易用的用于同时展示或可溶性分泌两种或更多种POI的系统。
可以根据本发明被替换的侧面结构域是相对大的，如具有20、30、40、60、80个或更多个氨基酸残基。
因此，自转运蛋白的载客结构域可以视为由非β茎部形成序列连接在一起的几段β茎部形成序列。这些非β茎部形成序列是用于插入一种或多种POI的合适位点。因此，可以将POI置于β茎部形成序列的两个部分之间。POI也可以与载客结构域的N端融合。
用检测自转运蛋白的载客结构域的β茎部形成序列和侧面结构域的合适方法包括生物物理方法如X射线结晶学和生物信息学软件如结构预测工具。
X射线结晶学当今是高度高效及自动化并且为本领域技术人员已知的标准方法。载客结构域的高分辨率结构和用于确定自转运蛋白的载客结构域的结构的合适方法的实例存在于(Otto等人，2005J Biol Chem(《生物化学杂志》)280(17):17339-45；Emsley等人，1996Nature(《自 然》)381：90-92；Johnson等人，2009J Mol Biol(《分子生物学杂志》)389(3)：559-74)中。
适于确定β茎部结构的生物信息学方法的实例是M4T同源性建模方法(Rykunov等人，2009J Struct Funct Genomics(《结构与功能基因组学杂志》)10：95-99)，该方法可在互联网上免费获得。
在蛋白质的三维模型用于鉴定β茎部结构域和侧面结构域的情况下，合适的是所获得的模型具有好于4埃的分辨率。侧面结构域随后将作为从β茎部突出的结构域可见到。通过观察自转运蛋白的载客结构域的结构，可以看到，序列的多个部分不是β茎部的部分，但形成从β茎部突出的结构域。
这些方法可以用于确定载客结构域的哪些结构域或氨基酸适于插入POI并且哪些应当保持基本上完整。
根据本发明，β茎部形成序列是基本上完整的。因此，应当尽可能少地移除β茎部形成序列。预测的结构域边界有助于确定POI应当在哪里插入。如果除去过多的β茎部形成序列，则分泌将不利地受到影响。β茎部形成序列基本上完整意指与破坏或完全除去β茎部相比时，蛋白质的分泌功能的效率维持在最佳水平。它也意指在分泌后，载客结构域的稳定性得到维持。本领域技术人员可以使用实验性方法来确定具体构建体是否允许高效分泌。
适于体外确定分泌效率的方法的实例包括：分析培养基中存在的POI的级分、用生物素或其他标记物标记表面蛋白、细胞分级、使表面蛋白暴露于蛋白酶(如蛋白酶K)和使用针对POI的抗体研究(如点印迹研究、免疫荧光显微术和免疫电子显微术)。
适于确定分泌后载客结构域的稳定性的方法的实例包括包括SDS-PAGE、蛋白质印迹法和上文在第76段下的全部方法。
因此，通过使用结构信息，本领域技术人员可以预测可在载客结构域中何处插入POI以便维持最佳分泌。可以用体外实验容易地测定实际分泌。
根据本发明，POI与载客结构域融合。这意指POI与形成载客结构域的肽融合，从而它们形成一条连续多肽。因为在DNA水平实施融合蛋白的设计，必须小心从事，从而POI的阅读框与载客结构域的阅读框相同。
优选地，POI具有小于200kD的分子量。更优选地，这个分子量小于200kD，如100kD、80kD、60kD、40kD、30kD、20kD、10kD或5kD。
融合蛋白可以包含多于一种POI。因此，融合蛋白可以包含2种、3种或多种感兴趣多肽。融合蛋白可以是这样的，从而它具有至少两种POI，每一种各自替代或部分替代或融合至载客结构域的独立或单独侧面结构域。可替代地，两种或更多种POI可以融合至、或替代或部分替代相同的侧面结构域。
融合蛋白由核酸编码并且在宿主细胞中表达。该核酸可以采用涉及限制性酶、DNA连接酶、PCR、寡核苷酸合成、DNA纯化的标准分子生物学技术和本领域技术人员熟知的其他方法构建。优选地，起点是自转运蛋白的阅读框，其中将编码POI的DNA片段插入该阅读框中，从而这些阅读框匹配。可替代地，融合蛋白的阅读框可以在计算机上设计并且使用多核苷酸合成法合成。
编码融合蛋白的阅读框优选地插在用于原核生物表达的表达载体中，该表达载体携带启动子和本领域技术人员熟知的其他元件。
融合蛋白包含指导蛋白质分泌的N端信号肽。当宿主细胞是革兰氏阴性细菌时，合适的信号肽是这样的，从而它指导蛋白质跨内膜易位。该信号肽可以源自自转运蛋白，合适地源自衍生载客结构域的相同自转运蛋白。该信号肽可以大致包含SEQ ID NO 1的第1至52位氨基酸或相似的序列。
融合蛋白适当地包含自伴侣结构域，其适当地来自用来融合POI的自转运蛋白的载客结构域。自伴侣结构域的一个实例大致包含包含SEQ ID NO 1的第1002至1100位氨基酸。
融合蛋白可以包含来自一个类型自转运蛋白的载客结构域和来自另一个类型自转运蛋白的易位蛋白结构域。
本发明中所用的自转运蛋白可以是具有丝氨酸蛋白酶结构域的自转运蛋白，如丝氨酸蛋白酶。
该自转运蛋白可以是SPATE蛋白(肠杆菌科的丝氨酸蛋白酶自转运蛋白)。因此，易位蛋白结构域和载客结构域可以来自SPATE蛋白。在一个实施例中，该SPATE蛋白是来自大肠杆菌的血红蛋白结合蛋白酶(Hbp)(SwissProt O88093)和温度敏感性血凝素(Tsh)(SwissProt Q47692)之一。Tsh的序列与Hbp的序列同源。
其他SPATE蛋白包括淋病奈瑟菌(Neisseria gonorrhoeae)和流感嗜血杆菌(Haemophilus influenzae)的IgA蛋白酶、来自大肠杆菌的EspC、来自大肠杆菌的Pet、来自大肠杆菌的EspP、来自大肠杆菌的Pic、来自大肠杆菌的PicU、来自大肠杆菌的Sat、来自大肠杆菌的Vat、来自大肠杆菌的EspI、来自大肠杆菌的EaaA、来自大肠杆菌的EaaC、来自大肠杆菌的EatA、来自大肠杆菌的EpeA、来自大肠杆菌的PssA、来自大肠杆菌的AidA_B7A、来自乍得沙门氏菌(Salmonella bongori)的Boa、来自福氏志贺氏菌(Shigella flexneri)的SepA、来自福氏志贺氏菌的SigA、来自福氏志贺氏菌的Pic。
该SPATE蛋白可以包含SEQ ID NO 1的多肽，该多肽是Hbp，或SEQ ID NO 2的多肽，该多肽是其中易位蛋白结构域和载客结构域之间的切割位点已经遭到破坏的Hbp(HbpΔ-cleav)，或与这些序列相似的序列。优选地，与这些序列的同一性多于80％、甚至更优选地多于90％、甚至更优选地多于95％并且最优选地多于97％。
这些SPATE组的蛋白质具有随本发明一起使用的几个优点。首先，它们的结构中一些是已知的，这利于鉴定它们的β茎部和侧面结构域。这种知识也可以用于预测晶体结构未知的相关SPATE的侧面结构域和β茎部结构。另一个优点是它们的切割结构，这种切割结构可以用于高效可溶性分泌并且在SPATE家族内部是保守的。
可以预测结构已知的其他自转运蛋白或它们将是已知的，从而它们的β茎部和侧面结构域结构可以确定，也可以随本发明一起使用。该自转运蛋白应当具有β茎部、侧面结构域和可任选地，高效用于可溶性分泌的切割系统。实例包括来自流感嗜血杆菌的自转运蛋白HapS，它不是SPATE家族的成员。最近已经发表HapS的载客结构域的结构(Meng等人，2011Aug 12The EMBO Journal(《EMBO杂志》),doi：10.1038/emboj.2011.279.[电子出版先于印刷版])。这种结构十分接近于Hbp的结构，具有一种具有4个侧面结构域的β-茎部(SD1-4)。
图11A显示自转运蛋白Hbp的载客结构域的晶体结构(Otto等人，2005J Biol Chem(《生物化学杂志》)280(17)：17339-45)。结构域1(d1)、结构域2(d2)和自伴侣结构域(ac)为浅灰色。载客结构域的其余部分(包括β茎部结构域)着色为黑色。结构域d1和d2均适于POI的插入。此外，图11C中所示的结构域d3和图11D中所示的结构域d4和d5适于POI的替换或插入。
结构域d1大致包含作为Hbp序列的SEQ ID NO 1的第53至308位氨基酸，d2大致包含第533-608位氨基酸，d3大致包含第657-697位氨基酸，d4大致包含第735至766位氨基酸，并且d5大致包含第898至922位氨基酸。
图11E显示野生型Hbp的结构域组成。此外，显示了在本文中所呈献的实施例中使用的融合蛋白。在野生型Hbp中，显示了包含β茎部(处于黑色)和侧面结构域d1、d2、d3、d4和d5的载客结构域。易位蛋白结构域位于蛋白质的C端部分处并且显示为“β-结构域”。“Ac”表示自伴侣结构域。信号肽由“ss”指示。数字指示从N端的氨基酸数。
可以使用大致包含SEQ ID NO 1第53-1100位氨基酸或相似序列的载客结构域。
可以使用大致包含SEQ ID NO 1第1101-1377位氨基酸或相似序列的易位蛋白结构域。
POI可以是分裂的蛋白质。分裂的蛋白质是一种蛋白质，该蛋白 质在其天然形式下包含单条多肽或由二硫桥或其他分子内键连接的几条多肽并且对于本发明而言已经分裂成两个或更多个部分。这类部分的每一个与载客结构域融合，从而它们例如相隔一定距离地形成一种非天然结构。这两个或更多个部分可以例如与不同侧面结构域融合或与相同侧面结构域融合但是相隔一定距离。将这类部分的每一个视为一种POI，从而将分裂的蛋白质视为两种或更多种POI。例如当天然蛋白具有抑制高效分泌的庞大或复杂结构，例如包含二硫桥时，这可能是有利的。使蛋白质分裂可以使分泌更高效。
POI可以包含至少一种抗原，例如来自感染性生物(如结核分枝杆菌)的抗原。来自结核分枝杆菌的这类抗原的实例包括ESAT-6样蛋白(例如ESAT-6、TB10.4、TB10.3)、Ag85B样蛋白(例如Ag85B)、和Rv2660c。两种或更多种的这类抗原可以与相同的载客结构域(例如与独立的侧面结构域)融合。
ESAT-6(6kDa早期分泌性抗原靶)是一种10kDa蛋白质，该蛋白质是有力的T-细胞抗原和重要的毒力因子。
Rv2660c是功能未知的7.6kDa胞内蛋白。
TB10.3和TB10.4均是具有96个氨基酸的蛋白质。
Ag85B是35kDa的分泌性分枝酰转移酶(mycolyltransferase)，包含3个半胱氨酸。它也是有力的T-细胞抗原。在其天然形式下，这种相当庞大和包含半胱氨酸的蛋白质对于利用自转运蛋白系统的最佳外膜易位而言太复杂。
在一个实施例中，该抗原如上文定义那样分裂。例如，Ag85B，作为庞大和相当复杂的蛋白质，可以分裂成N’部分(Ag85B(N’))和C’部分(Ag85B(C’))用于更高效的分泌。
在一个实施例中，POI包含一种多肽，该多肽具有与作为ESAT-6的序列的SEQ IDNO 39相似至少80％、更优选90％、更优选95％、最优选97％的序列。在一个实施例中，POI包含SEQ ID NO 39中定义的多肽。
在一个实施例中，POI包含一种多肽，该多肽具有与作为Rv2660c 的序列的SEQ IDNO 41相似至少80％、更优选90％、更优选95％、最优选97％的序列。在一个实施例中，POI包含SEQ ID NO 41中定义的多肽。
在一个实施例中，POI包含一种多肽，该多肽具有与作为TB10.4的序列的SEQ IDNO 42相似至少80％、更优选90％、更优选95％、最优选97％的序列。在一个实施例中，POI包含SEQ ID NO 42中定义的多肽。
在一个实施例中，POI包含一种多肽，该多肽具有与作为TB10.3的序列的SEQ IDNO 43相似至少80％、更优选90％、更优选95％、最优选97％的序列。在一个实施例中，POI包含SEQ ID NO 43中定义的多肽。
在一个实施例中，POI包含一种多肽，该多肽具有与作为Ag85B的序列的SEQ ID NO40的至少1/4相似至少80％、更优选90％、更优选95％、最优选97％的序列。在一个实施例中，POI包含由SEQ ID NO 40中的第1-126位或第118-285位氨基酸定义的多肽。
POI可以在侧翼分布有一个或多个接头区域。接头区域可以是1至20个或更多个氨基酸的柔性肽。该接头区域可以适当地在POI的C-端和N端处插入。接头的优点在于，它可以允许融合蛋白的多种结构域彼此更独立地移动。接头可以由本领域技术人员容易地设计。合适接头的实例包括SEQ ID NO 44和45。
融合蛋白可以包含在SEQ ID NO：13-19、SEQ ID NO：22-26或SEQ ID NO 38的任一者中定义的多肽或这些序列的任一个相似至少80％、更优选90％、更优选95％和最优选97％的多肽。
SEQ ID NO 13是Hbp的序列，其中ESAT6已经替换结构域d1(Hbp(Δd1)-ESAT6，又命名为HbpSL-ESAT6)。SEQ ID NO 14是相同的蛋白质，但是其中易位蛋白结构域和载客结构域之间的切割位点已经遭受破坏(HbpD(Δd1)-ESAT6，又命名为HbpDL-ESAT6)。SEQ IDNO 15是Hbp的序列，其中ESAT6已经替换结构域d2(Hbp(Δd2)-ESAT6)。SEQ ID NO 16是Hbp的序列，其中ESAT6已经替换结构域d2(Hbp(Δd2)-ESAT6，又命名为HbpDD2-ESAT6)并且其中易位蛋白结构域和载客结构域之间的切割位点已经遭受破坏。SEQ ID NO 17是Hbp的序列，其中 ESAT6已经替换结构域d3(Hbp(Δd3)-ESAT6)。SEQ ID NO 18是Hbp的序列，其中ESAT6已经替换结构域d4(Hbp(Δd4)-ESAT6)。SEQ ID NO 19是Hbp的序列，其中ESAT6已经替换结构域d5(Hbp(Δd5)-ESAT6)。
SEQ ID NO 22是Hbp的序列，其中Rv2660c已经替换结构域d3(Hbp(Δd3)-Rv2660c)。SEQ ID NO 23是Hbp的序列，其中Rv2660c已经替换结构域d4(Hbp(Δd4)-Rv2660c)。SEQ ID NO 24是Hbp的序列，其中Rv2660c已经替换结构域d5(Hbp(Δd5)-Rv2660c)。SEQ ID NO 25是Hbp的序列，其中TB10.4已经替换结构域d1(Hbp(Δd1)-TB10.4)。SEQ ID NO 26是Hbp的序列，其中TB10.3已经替换结构域d2(Hbp(Δd2)-TB10.3)。
SEQ ID NO 38是EspC的序列，其中ESAT6已经替换结构域d1(EspC(Δd1)-ESAT6)。
融合蛋白可以包含具有多于一种POI的多肽，如在SEQ ID NO：28-35的任一者中定义的多肽或这些序列的任一个相似至少80％、更优选90％、更优选95％和最优选97％的多肽。
SEQ ID NO 28是Hbp的序列，其中Ag85B的残基1-126已经替换结构域d1并且Ag85B的残基118-285已经替换结构域2(Hbp-Ag85B[N+C])。SEQ ID NO 29是Hbp的序列，其中Ag85B的残基1-126已经替换结构域d1并且Ag85B的残基118-285已经替换结构域2，并且其中易位蛋白结构域和载客结构域之间的切割位点已经遭受破坏(HbpD-Ag85B[N+C])。SEQ ID NO 30是Hbp的序列，其中Ag85B的残基1-126已经替换结构域d2并且Ag85B的残基118-285已经替换结构域1(Hbp-Ag85B[C+N])。SEQ ID NO 31是Hbp的序列，其中Ag85B的残基1-126已经替换结构域d2并且Ag85B的残基118-285已经替换结构域1，并且其中易位蛋白结构域和载客结构域之间的切割位点已经遭受破坏(HbpD-Ag85B[C+N])。
SEQ ID NO 32是Hbp的序列，其中Ag85B的残基1-126已经替换结构域d2，Ag85B的残基118-285已经替换结构域1并且ESAT6已经 替换结构域4(Hbp-Ag85B[C+N]-ESAT6)。SEQID NO 33是相同的蛋白质，但是其中易位蛋白结构域和载客结构域之间的切割位点已经遭受破坏(HbpD-Ag85B[C+N]-ESAT6)。[00118]SEQ ID NO 34是Hbp的序列，其中Ag85B的残基1-126已经替换结构域d2，Ag85B的残基118-285已经替换结构域1，ESAT6已经替换结构域4并且Rv2660c已经替换结构域5(Hbp-Ag85B[C+N]-ESAT6-Rv2660c)。SEQ ID NO 35是相同的蛋白质，但是其中易位蛋白结构域和载客结构域之间的切割位点已经遭受破坏(HbpD-Ag85B[C+N]-ESAT6-Rv2660c)。
优选地，融合蛋白的各结构域的顺序从N端至C端是：信号肽、载客结构域、易位蛋白结构域。
在本发明的第二方面，提供一种表达如本文定义的融合蛋白的细胞。该细胞优选地是可以通过本领域技术人员熟知的方法培养和操作并且能够表达异源蛋白质的宿主细胞。优选地，该宿主细胞是革兰氏阴性细菌如大肠杆菌、沙门氏菌属物种、弧菌属物种、志贺氏菌属物种、假单胞菌属物种、伯克霍尔德菌属物种或德特氏菌属物种多种表达系统是本领域技术人可获得和已知的。表达可以是稳定或瞬时性质的。表达系统可以是诱导型或非诱导型的。
在一个实施例中，融合蛋白由宿主细胞至少部分地可溶地分泌。当本发明用于产生例如作为商业酶或药品组分的重组蛋白时，可以使用这个实施例。POI随后可以在不破碎宿主细胞的情况下便利地从培养基中收获。破碎宿主细胞造成细胞残片和细胞内容物的污染。当融合蛋白在易位蛋白结构域和载客结构域之间包含蛋白酶切割位点时，可以实现融合蛋白的分泌。在易位蛋白结构域已经整合入外膜时，可以存在于融合蛋白自身内部的蛋白酶活性切割融合蛋白，从而载客结构域被释放至培养基中。可替代地，切割可以借助与蛋白酶活性无关的分子内自催化切割机制发生，如对于来自大肠杆菌的SPATE EspP(Dautin等人，2007EMBO J 26(7)：1942-1952)和来自大肠杆菌的AIDA-I(Charbonneau等人，2009,JBiol Chem(《生物化学杂志》)284(25)：17340-17353)所描述。
出于清晰目的，POI可以在一些情况下保持与细胞膜连接，即便 多肽已经被切割。这类连接通常将非共价性质的。
在一个实施例中，POI保持与易位蛋白结构域共价连接。在自转运蛋白的序列携带切割位点的情况下，可以通过突变易位蛋白结构域和载客结构域之间的切割位点实现这一点，从而切割事件不发生。因此，宿主细胞在细胞表面上展示融合蛋白的至少一部分，该部分包含至少一种POI。
在某些方面，本发明提供在其表面上展示根据本发明的融合蛋白的外膜小泡(OMV)或菌影。
在某些条件下，可以诱导革兰氏阴性细菌开始从其外膜排出小泡。这类外膜小泡(OMV)已经显示例如可用作疫苗平台。当携带抗原时，如同源自它们的母体细胞那样，这些小泡能够增强这类抗原的免疫原性。OMV可以容易地源自在其表面上展示本发明融合蛋白的革兰氏阴性细菌。用于产生和分离外膜小泡的方法是本领域已知的(Chen等人，2010PNAS107:3099-3104；Bernadac等人，1998J Bacteriol(《细菌学杂志》)180：4872-4878；Kesty和Kuehn 2004,J.Biol Chem(《生物化学杂志》)279：2069-2076)；Kolling和Matthews1999App Env Microbiol(《应用与环境微生物学》)65：1843-1848；Kitagawa等人，2010JBacteriol(《细菌学杂志》)192：5645-5656)。
类似地，菌影是一种非活疫苗平台。菌影是已经通过裂解(例如使用来自噬菌体PhiX174的致死性裂解基因E)清空其细胞质的细菌细胞包膜(Langemann等人，2010BioengBugs(《生物工程与昆虫》)1:326-336；Young 1992Microbiol rev(《微生物学综述》)56：430-481；Mayr等人，2005Adv Drug Deliv rev(《高级药物递送综述》)57：1381-1391)。菌影保留母体细胞的全部形态特征、结构特征和抗原性特征并且包含在裂解之前表达并锚定至细胞包膜的蛋白质。可以通过本发明的分泌系统和融合蛋白促进例如抗原性蛋白质的递送。
本发明的一个方面是一种包含根据本发明的融合蛋白、细胞、外膜小泡或菌影的疫苗。这种疫苗可以包含在细胞表面展示融合蛋白的宿主细胞，其中该融合蛋白包含至少一种POI。优选地，该POI则是如上文所 述的抗原。该宿主细胞可以是减毒的沙门氏菌菌株，如Curtiss R3rd等人，2010Crit Rev Immunol(《免疫学关键性综述》)30(3)：255-70中描述的菌株。该疫苗可以包含沙门氏菌活宿主细胞。
本发明的一个方面是编码如上文已经描述的根据本发明的融合蛋白的核酸。本发明的又一个方面是携带根据本发明的核酸的载体。
可以安排该核酸或载体用于表达多于一种的与相同载客结构域融合的POI。例如，编码载客结构域的序列可以包含允许框内地克隆至少两个编码POI的序列的至少两段克隆位点序列。这促进轻易克隆和表达任何所需的POI。可替代地，该核酸可以包含编码与载客结构域融合的POI的多于一个序列。
本发明的一个方面包括一种用于POI的分泌性蛋白表达的方法，该方法包括步骤：在宿主细胞中表达根据本发明的融合蛋白。表达载体是本领域技术人员熟知的。适当地，该载体具有适合于该宿主细胞的启动子，该启动子与编码根据本发明的融合蛋白的核酸可操作地连接。
这种方法可以包括步骤：鉴定自转运蛋白上的合适侧面结构域。这可以用上文描述的生物物理方法或生物信息学方法实施。
根据本发明的方法的一个方面包括步骤：用POI如此替换自转运蛋白的载客结构域的侧面结构域(或其部分)，以至于该自转运蛋白的载客结构域的β茎部形成序列是基本上完整的。可替代地，该方法可以包括步骤：将POI如此插入载客结构域中，以至于β茎部形成序列是基本上完整的。
该方法包括步骤：在其中编码融合蛋白的核酸翻译成许多融合蛋白分子并且融合蛋白分子进入分泌途径的条件下培养宿主细胞。
在一个实施例中，该方法包括另外的步骤：抑制宿主细胞中具有蛋白酶活性的周质酶，如DegP。可以通过缺失、打断或失活宿主细胞染色体上的DegP编码基因来抑制DegP的蛋白酶活性。可以通过在DegP的催化位点导入突变实施失活。抑制蛋白酶具有可以改进产率的优点。
在一个实施例中，该方法包括另外的步骤：下调宿主细胞的周质 间隙内催化蛋白质中二硫键形成的至少一种酶，如DsbA或DsbB。这具有可以改进产率的优点，尤其对于易于形成二硫桥的蛋白质，如真核来源的蛋白质。
在该方法的一个实施例中，POI以可溶性方式分泌。在该方法的一个实施例中，POI保持与细胞表面共价连接。
在一个实施例中，该方法包括又一个步骤：诱导小泡从宿主细胞的外膜排出，以产生在它们的表面上展示本发明的融合蛋白的外膜小泡。
在另一个实施例中，该方法包括另外的步骤：裂解革兰氏阴性细菌(例如使用来自噬菌体PhiX174的致死性裂解基因E裂解)，因此形成在它们的表面上展示融合蛋白的菌影。
本发明的一个最后方面包括根据本发明方法而可获得的融合蛋白。
实例
方法
菌株和培养基
先前已经描述过大肠杆菌菌株MC1061(araD139Δ(araA-leu)7697ΔlacX74galK16galE15(GalS)λ-e14-mcrA0relA1rpsL150(strR)spoT1mcrB1hsdR2)(Casadaban和Cohen 1980J Mol Biol(《分子生物学杂志》)138：179-207)。菌株TOP 10F’从Invitrogen公司获得。
在37℃在补充有0.2％葡萄糖的LB培养基中常规培育细胞。在0.4％葡萄糖存在下培育过夜培养物。在适宜的情况下，将细胞在氯霉素(30μg/mL)和链霉素(25μg/mL)或四环素(6.25μg/mL)存在时培育。
质粒的构建
质粒pEH3-Hbp(图1)携带全长hbp基因，该全长hbp基因的表达处于诱导型LacUV5启动子的控制下。这个质粒的构建已经在(Jong等人，2007Mol Microbiol(《分子微生物学》)63(5)：1524-1536)中 描述。
在图1-10中，易位蛋白结构域称作“β-结构域”。
表1：这项研究中使用的引物
质粒pEH3-Hbp[Δβ-cleav](图2)是携带hbp突变体的pEH3-Hbp(Jong等人，2007)衍生物，该hbp突变体编码一种形式的Hbp，其中载客结构域和易位蛋白结构域之间的天然切割位点已经在Gly和Ser残基分别置换氨基酸残基Asn1100和Asn1101时遭受破坏。pEH3-Hbp[Δβ-cleav]的构建已经在(Jong等人，2007Mol Microbiol(《分子微生物学》)63(5)：1524-1536)中描述。
质粒pHbpD(Δd1)，与pHbpDL相同，(图3)是携带hbp突变体的pEH3-Hbp[Δβ-cleav](Jong等人，2007Mol Microbiol(《分子微生物学》)63(5)：1524-1536)衍生物，该hbp突变体编码截短形式的Hbp[Δβ-cleav](Jong等人，2007Mol Microbiol(《分子微生物学》)63(5)：1524-1536)，其中全长Hbp氨基酸序列的氨基酸残基54-307已经由氨基酸序列Ser-Ser-Cys-Gly-Ser-Gly-Ser-Gly(SEQ ID NO 45)替换。编码后一种氨基酸序列的DNA序列含有SacI和BamHI限制性位点，这些限制性位点允许轻易地框内地将编码异源氨基酸序列的DNA序列克隆至HbpD(Δd1)编码序列中。为产生pHbpD(Δd1)，首先，产生pEH3-Hbp[Δβ-cleav](pEH3-Hbp[Δβ-cleav/ΔBamHI])变体，其在Hbp[Δβ-cleav]编码区内部和外部缺少BamHI限制性位点。随后，实施一个3步骤‘重叠延伸PCR’方法。在第一步骤中，使用pEH3-Hbp(Jong等人，2007Mol Microbiol(《分子微生物学》)63(5)：1524-1536)作为模板以及引物pEH_XbaI_Hbp fw和Hbp(Δdom1/Cas)rv，通过PCR扩增DNA片段。在第二步骤中，使用pEH3-Hbp(Jong等人，2007MolMicrobiol(《分子微生物学》)63(5)：1524-1536)作为模板以及引物Hbp(Δdom1/Cas)fw和Hbp1123-1104rv，通过PCR扩增DNA片段。在第三步骤中，使用来自步骤1和2的PCR产物的混合物作为模板以及引物pEH_XbaI_Hbp fw和Hbp1123-1104rv，通过PCR扩增DNA片段。使用XbaI和NdeI限制性位点，将源自步骤3的PCR产物克隆至pEH3-Hbp[Δβ-cleav/ΔBamHI]中，产生质粒pHbpD(Δd1)。
对于这项研究中使用的引物，见表1。
根据与pHbpD(Δd1)相同的一般方法，产生质粒pHbpD(Δd2)，其与pHbpDD2相同(图5)但是具有以下修饰：全长Hbp氨基酸序列的氨基酸残基534-607由氨基酸序列Gly-Ser-Gly-Ser-Ser-Ala-Gly-Ser-Gly-Ser-Gly(SEQ ID NO 44)替换。编码这种氨基酸序列的DNA序列还含有SacI和BamHI限制性位点，这些限制性位点用于轻易地框内地克隆编码异源氨基酸序列的DNA序列。对于第一PCR扩增步骤，使用引物Hbp944-962fw和Hbp(Δdom2/Cas)rv。对于第二PCR扩增步骤，使用引物Hbp(Δdom2/Cas)fw和Hbp 2154-2137rv。并且对于第三步骤，使用引物HHbp944-962fw和Hbp2154-2137rv。使用NdeI和NsiI限制性位点，将源自步骤3的PCR产物克隆至pEH3-Hbp[Δβ-cleav/ΔBamHI]中。
质粒pHbp(Δd1)，与pHbpSL相同，(图7)是携带hbp突变体的pEH3-Hbp(Jong等人，2007Mol Microbiol(《分子微生物学》)63(5)：1524-1536)衍生物，该hbp突变体编码截短形式的Hbp[pHbp(Δd1)]，其中全长Hbp氨基酸序列的氨基酸残基54-307已经由氨基酸序列Ser-Ser-Cys-Gly-Ser-Gly-Ser-Gly(SEQ ID NO 45)替换。编码后一种氨基酸序列的DNA序列含有SacI和BamHI限制性位点，这些限制性位点允许轻易地框内地将编码异源氨基酸序列的DNA序列克隆至Hbp(Δd1)编码序列中。为构建pHbp(Δd1)，首先产生pEH3-Hbp(pEH3-Hbp/ΔBamHI)变体，其缺少hbp ORF下游的BamHI位点。随后，实施一个3步骤‘重叠延伸PCR’方法。在第一步骤中，使用pEH3-Hbp(Jong等人，2007Mol Microbiol(《分子微生物学》)63(5)：1524-1536)作为模板以及引物pEH_XbaI_Hbp fw和Hbp(Δdom1/Cas)rv，通过PCR扩增DNA片段。在第二步骤中，使用pEH3-Hbp(Jong等人，2007Mol Microbiol(《分子微生物学》)63(5)：1524-1536)作为模板以及引物Hbp(Δdom1/Cas)fw和Hbp1123-1104rv，通过PCR扩增DNA片段。在第三步骤中，使用来自步骤1和2的PCR产物的混合物作为模板以及引物pEH_XbaI_Hbp fw和Hbp1123-1104rv，通过PCR扩增DNA片段。使用Xba I和NdeI限制 性位点，将源自步骤3的PCR产物克隆至pEH3-Hbp[ΔBamHI](缺少hbp基因下游的BamHI限制性位点的pEH3-Hbp衍生物)中，产生质粒pHbp(Δd1)。
根据与pHbpD(Δd1)相同的一般方法，产生质粒pHbpSS(图9)、pHbp(Δd2)、pHbp(Δd3)、pHbp(Δd4)、pHbp(Δd5)、pHbp(d4ins)和pHbp(βins)，但是具有以下修饰。
对于pHbpSS：全长Hbp氨基酸序列的氨基酸残基54-993由氨基酸序列Ser-Ser-Cys-Gly-Ser-Gly-Ser-Gly(SEQ ID NO 45)替换。对于第一PCR扩增步骤，使用引物pEH_XbaI_Hbp fw和Hbp(Δdom1/Cas)rv。对于第二PCR扩增步骤，使用引物Hbp(Δβ-茎部/Cas)fw和EcoRI_Hbp rv。并且对于第三步骤，使用引物pEH_XbaI_Hbp fw和EcoRI_Hbp rv。使用XbaI和NdeI限制性位点，将源自步骤3的PCR产物克隆至pEH3-Hbp[ΔBamHI]中，产生质粒pHbpSS。
对于pHbp(Δd2)：全长Hbp氨基酸序列的氨基酸残基534-607由氨基酸序列Gly-Ser-Gly-Ser-Ser-Ala-Gly-Ser-Gly-Ser-Gly(SEQ ID NO 44)替换，其相应的DNA序列含有SacI和BamHI限制性位点，这些限制性位点用于轻易地框内地克隆DNA序列。对于第一PCR扩增步骤，使用引物Hbp944-962fw和Hbp(Δdom2/Cas)rv。对于第二PCR扩增步骤，使用引物Hbp(Δdom2/Cas)fw和Hbp 2154-2137rv。并且对于第三步骤，使用引物HHbp944-962fw和Hbp2154-2137rv。使用NdeI和NsiI限制性位点，将源自步骤3的PCR产物克隆至pEH3-Hbp[ΔBamHI]中，产生质粒pHbp(Δd2)。
对于pHbp(Δd3)：全长Hbp氨基酸序列的氨基酸残基659-696由氨基酸序列Gly-Ser-Gly-Ser-Ser-Ala-Gly-Ser-Gly-Ser-Gly(SEQ ID NO 44)替换。对于第一PCR扩增步骤，使用引物Hbp944-962fw和Hbp(Δdom3/Cas)rv。对于第二PCR扩增步骤，使用引物Hbp(Δdom3/Cas)fw和Hbp 2838-2820rv。并且对于第三步骤，使用引物HHbp944-962fw和Hbp2838-2820rv。使用NdeI和KpnI限制性位点，将源自步骤3的PCR产物克隆至pEH3-Hbp[ΔBamHI]中，产生质粒pHbp(Δd3)。
对于pHbp(Δd4)：全长Hbp氨基酸序列的氨基酸残基736-765由氨基酸序列Gly-Ser-Gly-Ser-Ser-Ala-Gly-Ser-Gly-Ser-Gly(SEQ ID NO 44)替换。对于第一PCR扩增步骤，使用引物Hbp 1859-1879fw和Hbp(Δdom4/Cas)rv。对于第二PCR扩增步骤，使用引物Hbp(Δdom4/Cas)fw和Hbp 2838-2820rv。并且对于第三步骤，使用引物Hbp1859-1879fw和Hbp2838-2820rv。使用NsiI和KpnI限制性位点，将源自步骤3的PCR产物克隆至pEH3-Hbp[ΔBamHI]中，产生质粒pHbp(Δd4)。
对于pHbp(Δd5)：全长Hbp氨基酸序列的氨基酸残基899-920由氨基酸序列Gly-Ser-Gly-Ser-Ser-Ala-Gly-Ser-Gly-Ser-Gly(SEQ ID NO 44)替换。对于第一PCR扩增步骤，使用引物Hbp1859-1879fw和Hbp(Δdom5/Cas)rv。对于第二PCR扩增步骤，使用引物Hbp(Δdom5/Cas)fw和Hbp3003-3021rv。并且对于第三步骤，使用引物Hbp1859-1879fw和Hbp3003-3021rv。使用NsiI和KpnI限制性位点，将源自步骤3的PCR产物克隆至pEH3-Hbp[ΔBamHI]中，产生质粒pHbp(Δd5)。
对于pHbp(d4ins)：全长Hbp氨基酸序列的氨基酸残基760-764由氨基酸序列Gly-Ser-Gly-Ser-Ser-Ala-Gly-Ser-Gly-Ser-Gly(SEQ ID NO 44)替换。对于第一PCR扩增步骤，使用引物Hbp1859-1879fw和Hbp(d4ins/Cas)rv。对于第二PCR扩增步骤，使用引物Hbp(d4ins/Cas)fw和Hbp 2838-2820rv。并且对于第三步骤，使用引物Hbp1859-1879fw和Hbp2838-2820rv。使用NsiI和KpnI限制性位点，将源自步骤3的PCR产物克隆至pEH3-Hbp[ΔBamHI]中，产生质粒(d4ins)。
对于pHbp(βins)：将氨基酸序列Gly-Ser-Gly-Ser-Ser-Ala-Gly-Ser-Gly-Ser-Gly(SEQ ID NO 44)插入全长Hbp氨基酸序列的残基771和772之间。对于第一PCR扩增步骤，使用引物Hbp1859-1879fw和Hbp(βins/Cas)rv。对于第二PCR扩增步骤，使用引物Hbp(βins/Cas)fw和Hbp 2838-2820rv。并且对于第三步骤，使用引物Hbp1859-1879fw和Hbp2838-2820rv。使用NsiI和KpnI限制性位点，将源自步骤3的PCR产物克隆至pEH3-Hbp[ΔBamHI]中，产生质粒pHbp(βins)。
通过对应于结核分枝杆菌ESAT6蛋白的异源插入物插入相应的质粒中，衍生以上质粒的ESAT6衍生物。为构建ESAT6衍生物，从BaseClear B.V.(Leiden，荷兰)获得编码ESAT6的合成DNA序列，该合成DNA序列的密码子使用针对在大肠杆菌中表达而进行优化。该合成DNA片段在ESAT6编码序列的5’侧和3’侧处分别拥有SacI和BamHI位点。这允许克隆至pHbpD(Δd1)、pHbpD(Δd2)、pHbp(Δd1)、pHbpSS、pHbp(Δd2)、pHbp(Δd3)、pHbp(Δd4)、pHbp(Δd5)、pHbp(d4ins)和pHbp(βins)的SacI和BamHI位点中，分别产生pHbpD(Δd1)-ESAT6(其与pHbpDL-ESAT6相同)(图4)、pHbpD(Δd2)-ESAT6(其与pHbpDD2-ESAT6相同)(图6)、pHbp(Δd1)-ESAT6(其与pHbpSL-ESAT6相同)(图8)、pHbpSS-ESAT6(图10)、pHbp(Δd2)-ESAT6、pHbp(Δd3)-ESAT6、pHbp(Δd4)-ESAT6、pHbp(Δd5)-ESAT6、pHbp(d4ins)-ESAT6和pHbp(βins)-ESAT6。
通过对应于结核分枝杆菌Rv2660c蛋白的异源插入物插入相应的质粒中，衍生以上质粒的Rv2660c衍生物。为构建Rv2660c衍生物，使用结核分枝杆菌H37Rv基因组DNA作为模板，通过PCR扩增带有侧翼SacI/BamHI位点的编码Rv2660c的基因。所用的引物是Cas/Rv2660c fw和Cas/Rv2660c rv。使用SacI/BamHI位点，将PCR产物分别克隆至pHbp(Δd3)，pHbp(Δd4)和pHbp(Δd5)中，产生pHbp(Δd3)-Rv2660c，pHbp(Δd4)-Rv2660c和pHbp(Δd5)-Rv2660c。
通过对应于结核分枝杆菌蛋白TB10.3或TB10.4的异源插入物插入相应的质粒中，衍生以上质粒的TB10.3和TB10.4衍生物。为构建TB10.3和TB10.4衍生物，使用结核分枝杆菌H37Rv基因组DNA作为模板，通过PCR扩增带有侧翼SacI/BamHI位点的编码TB10.3或TB10.4的基因。用于TB10.3的引物是Cas/TB10.3fw和Cas/TB10.3rv。使用SacI/BamHI位点，将PCR产物克隆至pHbp(Δd2)中，产生pHbp(Δd2)-TB10.3。用于TB10.4的引物是Cas/TB10.4fw和Cas/TB10.4rv。使用SacI/BamHI位点，将PCR产物克隆至pHbp(Δd1)中，产生pHbp(Δd1)-TB10.4。
质粒pHbp(Δd1)-hEGF(0ss)是一种pHbp(Δd1)衍生物，其表达含有与无半胱氨酸形式的智人(Homo sapiens)hEGF蛋白相对应的异源插入物的Hbp(Δd1)。为构建pHbp(Δd1)-hEGF(0ss)，获得编码hEGF(0ss)的合成DNA序列，该合成DNA序列在hEGF(0ss)编码序列的5’和3’侧分别拥有SacI和BamHI位点以允许克隆至pHbp(Δd1)的SacI和BamHI位点中，从而产生pHbp(Δd1)-hEGF(0ss)。
质粒pHbp-Ag85B(N+C)是表达Hbp突变体的pEH3-Hbp/ΔBamHI衍生物，其中，与来自结核分枝杆菌的蛋白Ag85B的成熟区域的残基1-126(Ag85B(N’))相对应的氨基酸序列插入如对pHbp(Δd1)所述那样定位的柔性接头中。此外，与蛋白Ag85B的成熟区域的残基118-285(Ag85B(C’))相对应的氨基酸序列插入如对pHbp(Δd2)该那样定位的柔性接头中。为构建pHbp-Ag85B(N+C)，使用结核分枝杆菌H37Rv基因组DNA作为模板，产生带有侧翼SacI/BamHI位点的编码Ag85B(N)和Ag85B(C)的fbpA片段。对于Ag85B(N)，所用的引物是Cas/Ag85B fw和Cas/Ag85B(S126)rv。使用SacI/BamHI限制性位点，将所得到的PCR片段克隆至pHbp(Δd1)中，产生pHbp(Δd1)-Ag85B(N)。对于Ag85B(C)，所用的引物是Cas/Ag85B(T118)fw和Cas/Ag85B rv。使用SacI/BamHI限制性位点，将所得到的PCR片段插入pHbp(Δd2)中，产生pHbp(Δd2)-Ag85B(C)。随后，pHbp(Δd2)-Ag85B(C)的XbaI/NdeI片段由pHbp(Δd1)-Ag85B(N)的XbaI/NdeI片段替换，产生pHbp-Ag85B(N+C)。
根据与pHbp-Ag85B(N+C)相同的一般方法，产生质粒pHbp-Ag85B(C+N)、pHbpD-Ag85B(N+C)和pHbpD-Ag85B(C+N)，但是具有以下修饰。
对于pHbp-Ag85B(C+N)，将来自结核分枝杆菌的蛋白Ag85B的成熟区域的N端部分(残基1-126)(Ag85B(N’))插入如对pHbp(Δd2)所述那样定位的柔性接头中并且将C端部分(残基118-285)插入如对pHbp(Δd1)所述那样定位的柔性接头中。在PCR后，使用与上文相同的引物，利用SacI/BamHI限制性位点，将Ag85B(N)PCR产物克隆至pHbp(Δd2)中，并且利用SacI/BamHI限制性位点，将Ag85B(c)PCR产物克隆至pHbp (Δd1)中，分别产生pHbp(Δd2)-Ag85B(N)和pHbp(Δd1)-Ag85B(C)。随后，pHbp(Δd2)-Ag85B(N)的XbaI/NdeI片段由pHbp(Δd1)-Ag85B(C)的XbaI/NdeI片段替换，产生pHbp-85B(C+N)。
对于pHbpD-Ag85B(N+C)和pHbpD-Ag85B(C+N)，分别使用如用于pHbpD-Ag85B(N+C)和pHbpD-Ag85B(C+N)的相同方法，例外在于使用质粒pHbpD(Δd1)和pHbpD(Δd2)而非质粒pHbp(Δd1)和pHbp(Δd2)。
质粒pHbp-Ag85B[C+N]-ESAT6是pHbp-Ag85B[C+N]的衍生物，其编码一种形式的Hbp-Ag85B[C+N]，其中与ESAT6相对应的氨基酸序列插入如对pHbp(d4ins)所述那样定位的柔性接头中。为构建pHbp-Ag85B[C+N]-ESAT6,pHbp-Ag85B[C+N]的NsiI/KpnI片段由pHbp(d4in)-ESAT6的相应片段替换，从而产生pHbp-Ag85B[C+N]-ESAT6。相应地产生质粒pHbpD-Ag85B[C+N]-ESAT6，例外在于pHbpD-Ag85B[C+N]的NsiI/KpnI片段由pHbp(d4in)-ESAT6的相应片段替换。
质粒pHbp-Ag85B[C+N]-ESAT6-Rv2660c是pHbp-Ag85B[C+N]-ESAT6的衍生物，其编码一种形式的Hbp-Ag85B[C+N]-ESAT6，其中与Rv2660c相对应的氨基酸序列插入如对pHbp(Δd5)所述那样定位的柔性接头中。为构建pHbp-Ag85B[C+N]-ESAT6-Rv2660c,pHbp-Ag85B(C+N)-ESAT6的BstZ17i/KpnI片段由pHbp(Δd5)-Rv2660c的相应片段替换，从而产生pHbp-Ag85B(C+N)-ESAT6-Rv2660c。相应地产生质粒pHbpD-Ag85B(C+N)-ESAT6-Rv2660c，例外在于pHbpD-Ag85B(C+N)-ESAT6的BstZ17i/KpnI片段由pHbp(Δd5)-Rv2660c的相应片段替换。
质粒pEH3-EspC携带全长espC基因，该全长espC基因的表达处于诱导型LacUV5启动子的控制下。为构建pEH3-EspC，使用pJLM174(Dutta等人，2002Infect.Immun(《感染免疫学》).70,7105-7113)作为模板以及引物pEH_XbaI_EspC fw和EcoRI_EspC rv，通过PCR扩增espC基因。将所得到的PCR产物克隆至pEH3-Hbp的XbaI/EcoRI位点中。这个步骤有效地把espC ORF交换为hbp的ORF，产生pEH3-EspC。
质粒pEH3-EspC(Δd1)是携带espC突变体的pEH3-EspC衍生物，该espC突变体编码截短形式的EspC，其中全长EspC氨基酸序列的氨基酸残基54-300已经由氨基酸序列Gly-Ser-Ser-Ala-Gly-Ser-Gly-Ser-Gly(SEQ ID NO 46)替换。编码后一种氨基酸序列的DNA序列含有SacI和BamHI限制性位点，这些限制性位点允许轻易地框内地将编码异源氨基酸序列的DNA序列克隆至EspC(Δd1)编码序列中。为产生pEH3-EspC(Δd1)，实施一个3步骤‘重叠延伸PCR’方法。在第一步骤中，使用pEH3-EspC作为模板以及引物EH_XbaI_EspC fw和EspC(Δdom1/Cas)rv，通过PCR扩增DNA片段。在第二步骤中，使用pEH3-EspC作为模板以及引物EspC(Δdom1/Cas)fw和EspC(BglII)rv，通过PCR扩增DNA片段。在第三步骤中，使用来自步骤1和2的PCR产物的混合物作为模板以及引物pEH_XbaI_EspC fw和EspC(BglII)rv，通过PCR扩增DNA片段。使用XbaI和BglII限制性位点，将源自步骤3的PCR产物克隆至pEH3-EspC中，产生质粒pEH3-EspC(Δd1)。
质粒pEH3-EspC(Δd1)-ESAT6是一个pEH3-EspC(Δd1)衍生物，其表达含有与结核分枝杆菌病ESAT6蛋白相对应的异源插入物的EspC(Δd1)。为构建pEH3-EspC(Δd1)-ESAT6，如上文所述获得编码ESAT6的合成DNA序列，该DNA序列在5’和3’侧拥有SacI和BamHI位点。这允许将编码ESAT6的合成DNA序列克隆至pEH3-EspC(Δd1)的SacI和BamHI位点中，产生pEH3-EspC(Δd1)-ESAT6。
质粒pEH3(p15a)-HbpD-Ag85B[C+N]-ESAT6(图34)与质粒pHbpD-Ag85B[C+N]-ESAT6相同，除了携带pMB1复制起点的DNA片段已经由携带p15a复制起点的片替换之外。为产生pEH3(p15a)-HbpD-Ag85B[C+N]-ESAT6，首先产生质粒pEH3(p15a)-Hbp。使用pBAD33(Guzman等人，1995Journal of Bacteriology(《细菌学杂志》)177:4121-4130)作为模板以及引物p15afw和p15a rv(见表1)，产生这种质粒。使用SalI/EcoRI限制性位点，将所得到的携带p15a复制起点的PCR片段克隆至pEH3-Hbp中，产生pEH3(p15a)-Hbp。随后，pEH3(p15a)-Hbp的XbaI/EcoRI片段由pHbpD-Ag85B[C+N]-ESAT6的相应片段替换，产生 pEH3(p15a)-HbpD-Ag85B[C+N]-ESAT6。
对实例中所用构建体的描述
对于更详细的描述和怎样产生构建体，见上文“质粒的构建”下的内容。
图11A显示自转运蛋白Hbp的载客结构域的晶体结构(Otto等人，2005J Biol Chem(《生物化学杂志》)280(17)：17339-45)。结构域1(d1)、结构域2(d2)和自伴侣结构域(ac)为浅灰色。载客结构域的其余部分，包括β茎部结构域，着色为黑色。
图11B显示自转运蛋白Hbp的载客结构域的晶体结构(Otto等人，2005J Biol Chem(《生物化学杂志》)280(17)：17339-45)，与图11A中描述的位置相比，绕y-轴转动(50°逆时针)。使用MacPyMol创建本图像。
图11C显示如图11B中那样的自转运蛋白Hbp的载客结构域的晶体结构，但是构成结构域1的残基隐藏。结构域3(d3)以浅灰色描绘。载客结构域的其余部分着色为黑色。使用MacPyMol创建本图像。
图11D显示如图11C中那样的自转运蛋白Hbp的载客结构域的晶体结构。结构域4(d4)和对应于Hbp残基898-922的侧面结构域(d5)以浅灰色描绘。载客结构域的其余部分着色为黑色。使用MacPyMol创建本图像。
图11E显示实例中所用的Hbp衍生构建体的示意图。对于本文中公开的实例，将图11E中所示的Hbp衍生构建体克隆至中质粒pEH3-Hbp[ΔBamHI]或pEH3-Hbp[Δβ-cleav/ΔBamHI]，因此形成与图3-10中所示的载体相对应的表达载体。图23显示这些实例中所用的EspC衍生构建体的示意图。对于构建体的SEQ ID NO:，见表2。
表2：这项研究中使用的构建体
将Hbp(野生型)作为1377个氨基酸(aa)的前体合成，该前体以3个结构域构成：(i)N端可切割的信号序列(ss；aa 1-52)，(ii)载客结构域(aa 53-1100)和(iii)外膜整合的C端易位蛋白结构域(β-结构域；aa 1101-1377)。显示载客结构域的结构域1(d1)、结构域2(d2)、结构域3(d3)、结构域4(d4)、结构域5(d5)和自伴侣结构域(ac)。“FL”指柔性接头。载客结构域的其余部分(包括β茎部结构域)着色为黑色。在通过外膜后，载客结构域借助自催化机制被从易位蛋白结构域切下，该自催化机制涉及水解Hbp前体的Asn1100和Asn1101之间的肽键。上文示意图中所示的数字对应于原始Hbp(野生型)前体的氨基酸位置，从N端算起。
“E-6”表示ESAT6。“26”表示Rv2660c。“10.3”表示TB10.3并且“10.4”表示TB10.4”。”EGF”表示hEGF(0ss)。“85[N]”表示Ag85B[N’]并且“85[C]”表示Ag85B[C’]。
Hbp(Δβ-cleav)代表Hbp(野生型)的突变体，其中载客结构域不能被从易位蛋白结构域切割，原因在于，Gly和Ser残基分别置换Asn1100和Asn1101破坏了切割位点(黑色叉)。
HbpSS代表Hbp的突变体，其中除自伴侣结构域之外，绝大部分的载客结构域已经由柔性接头(FL)替换，该柔性接头允许插入异源蛋白质序列。
Hbp(Δd1)、Hbp(Δd2)、Hbp(Δd3)、Hbp(Δd4)和Hbp(Δd5)代表Hbp的突变体，其中载客结构域的结构域1、2、3、4和5已经分别地由柔性接头替换。Hbp(Δd1)与HbpSL相同。
HbpD(Δd1)和HbpD(Δd2)分别与Hbp(Δd1)和Hbp(Δd2)相同，例外在于载客结构域和易位蛋白结构域之间的切割位点如对Hbp(Δβ-cleav)所述那样遭破坏。HbpD(Δd1)已经还命名为HbpDL并且HbpD(Δd2)已经命名为HppDD2。
Hbp(d4ins)是Hbp的突变体，其中位于结构域4中的残基760-764已经由柔性接头替换。
HbpSS-ESAT6、Hbp(Δd1)-ESAT6、HbpD(Δd1)-ESAT6、Hbp(Δd2)-ESAT6、HbpD(Δd2)-ESAT6、Hbp(Δd3)-ESAT6、Hbp(Δd4)-ESAT6、Hbp(Δd5)-ESAT6和Hbp(d4ins)-ESAT6分别是HbpSS、Hbp(Δd1)、HbpD(Δd1)、Hbp(Δd2)、HbpD(Δd2)、Hbp(Δd3)、Hbp(Δd4)、Hbp(Δd5)和Hbp(d4ins)的衍生物。在这些衍生物中，与结核分枝杆菌蛋白ESAT6相对应的氨基酸序列插入柔性接头中，从而在天然Hbp序列和ESAT6的N’端及C’之间留下包含Gly和Ser残基的短柔性间隔区。
Hbp(βins)-ESAT6是Hbp的突变体，其中与ESAT6和短N’和C’侧翼柔性间隔区相对应的氨基酸序列已经插入Hbp载客结构域的β-链形成序列中：在残基771和772之间。
Hbp(Δd3)-Rv2660c、Hbp(Δd4)-Rv2660c和Hbp(Δd5)-Rv2660c分别是Hbp(Δd3)、Hbp(Δd4)和Hbp(Δd5)的衍生物。在这些衍生物中，与结核分枝杆菌蛋白Rv2660c相对应的氨基酸序列插入如分别对Hbp(Δd3)、Hbp(Δd4)和Hbp(Δd5)所述那样的柔性接头中。
Hbp(Δd1)-TB10.4是Hbp(Δd1)的衍生物，其中与结核分枝杆菌蛋白TB10.4相对应的氨基酸序列插入如对Hbp(Δd1)所述那样的柔性接头中。
Hbp(Δd2)-TB10.3是Hbp(Δd2)的衍生物，其中与结核分枝杆菌蛋白TB10.3相对应的氨基酸序列插入如对Hbp(Δd2)所述那样的柔性接头中。
Hbp(Δd1)-hEGF(0ss)是Hbp(Δd1)的衍生物，其中与智人蛋白hEGF的无半胱氨酸突变体相对应的氨基酸序列插入如对Hbp(Δd1)所述那样的柔性接头中。
Hbp-Ag85B[N+C]是Hbp的突变体，其中，与来自结核分枝杆菌的蛋白Ag85B的成熟区域的残基1-126相对应的氨基酸序列(Ag85B[N’])插 入如对Hbp(Δd1)所述那样定位的柔性接头中。此外，与蛋白Ag85B的成熟区域的残基118-285相对应的氨基酸序列(Ag85B[C’])插入如对Hbp(Δd2)所述那样定位的柔性接头中。
Hbp-Ag85B[C+N]是Hbp的突变体，其中，与来自结核分枝杆菌的蛋白Ag85B的成熟区域的残基1-126相对应的氨基酸序列(Ag85B[N’])插入如对Hbp(Δd2)所述那样定位的柔性接头中。此外，与蛋白Ag85B的成熟区域的残基118-285相对应的氨基酸序列(Ag85B[C’])插入如对Hbp(Δd1)所述那样定位的柔性接头中。
Hbp-Ag85B[C+N]-ESAT6是Hbp-Ag85B[C+N]的衍生物，其中与ESAT6相对应的氨基酸序列插入如对Hbp(d4ins)所述那样定位的柔性接头中。因此，Ag85B[C’]在d1处插入，Ag85B[N’]在d2处插入并且ESAT6在d4处插入。
Hbp-Ag85B[C+N]-ESAT6-Rv2660c是Hbp-Ag85B[C+N]-ESAT6的衍生物，其中与Rv2660c相对应的氨基酸序列插入如对Hbp(Δd5)所述那样定位的柔性接头中。因此，Ag85B[C’]在d1处插入，Ag85B[N’]在d2处插入，ESAT6在d4处插入并且Rv2660c在d5处插入。
HbpD-Ag85B[C+N]、HbpD-Ag85B[C+N]-ESAT6和HbpD-Ag85B[C+N]-ESAT6-Rv2660c分别是Hbp-Ag85B[C+N]、Hbp-Ag85B[C+N]-ESAT6和Hbp-Ag85B[C+N]-ESAT6-Rv2660c的衍生物，例外在于载客结构域和易位蛋白结构域之间的切割位点如对Hbp(Δβ-cleav)所述那样遭破坏。
一般方法
使用伴有MES-SDS运行缓冲液的4％-12％NuPAGE Bis-Tris凝胶(Invitrogen公司)进行SDS-PAGE。可替代地，使用10％、4％-15％或‘任何-kD’Biorad mini-Protean TGX凝胶或标准10％SDS-PAGE凝胶进行SDS-PAGE。在进行SDS-PAGE之前，将蛋白质样品溶解于SDS-PAGE样品缓冲液(63mM TrisHcl pH 6.8，2％w/v SDS，10％甘油，0.01％w/v溴 酚蓝，41mM DTT)中并且煮沸5分钟。凝胶用考马斯亮兰G-250染色并且使用Molecular ImagerGS-800Calibrated Densitometer(Biorad公司)捕获。可替代地，对凝胶进行蛋白质印迹法。在适宜的情况下，蛋白质印迹物与针对Hbp载客结构域、Hbp易位蛋白结构域或外膜蛋白OmpA的兔多克隆抗体孵育。可替代地，蛋白质印迹物与针对结核分枝杆菌ESAT6或Ag85B的小鼠单克隆抗体或与针对Rv2660c的大鼠多克隆抗血清孵育。随后，在需要的情况下，蛋白质印迹物与辣根过氧化物酶(HRP)缀合的山羊抗兔抗体(Rockland Immunochemicals公司)、HRP缀合的兔抗小鼠抗体或HRP缀合的兔抗大鼠抗体孵育。使用化学发光LumiLight蛋白质印迹底物(Roche公司)，将蛋白质印迹物显影。检测化学发光信号并且使用ChemiDocXRS+Molecular Imager(BioRad公司)将其数字化。
实例1
Hbp分泌和展示构建体的表达和生物生成(图12)。
本实例显示经设计用于分泌或展示异源氨基酸序列的Hbp构建体的正确表达和生物生成。恰当地加工在载客结构域和易位蛋白结构域(HbpSS和HbpSL)之间携带完整切割位点的构建体，产生了整合至外膜中的易位蛋白结构域和分泌至培养基中的载客结构域。在载客结构域和易位蛋白结构域(HbpDL和HbpDD2)之间携带遭破坏的切割位点的构建体的表达产生了保持与易位蛋白结构域共价连接并且因此与细胞连接的载客结构域。
Hbp、Hbp(Δβ-cleav)、HbpSS、HbpSL(又命名为Hbp(Δd1))、HbpDL(也命名为HbpD(Δd1))和HbpDD2(又命名为HbpD(Δd2))的表达和分泌。在新鲜的培养基中继代培养来自过夜培养物的大肠杆菌MC1061细胞(该MC1061细胞携带克隆至表达载体pEH3中的构建体)，它们的生长得以继续。当培养物达到早对数期(OD660≈0.3)时，用1mM IPTG诱导Hbp(衍生物)的表达。从诱导后2小时的培养物收集样品，并且通过低速离心将细胞(c)和用过的培养基(m)分离。细胞直接溶解于 SDS-PAGE样品缓冲液中，而培养基样品首先进行TCA沉淀。通过SDS-PAGE和考马斯染色分析与0.03OD660单位相对应的细胞样品(A)。通过SDS-PAGE和蛋白质印迹法分析与0.03OD660单位相对应的细胞样品，该蛋白质印迹法使用针对全长Hbp载客结构域的多克隆抗体(B)或针对Hbp易位蛋白结构域的N端表位的多克隆抗体(C)。在各小图的左侧展示分子质量(kDa)标记。显示了构建体的经加工的载客结构域(>)、经加工的易位蛋白结构域(#)和包含载客结构域及易位蛋白结构域的未加工的原形式(*)。
实例2
携带异源蛋白质的Hbp分泌构建体的表达和生物生成(图13)。
本实例显示，与Hbp载客结构域在载客结构域的结构域1的位置处融合(HbpSL-ESAT6)时，异源蛋白质ESAT6借助Hbp自转运蛋白系统高效转运至胞外环境(培养基)。本实例也展示对于异源蛋白质的分泌，需要保持Hbp载客结构域的β茎部完整。这由以下观察结果而来：与其载客结构域已经被N’截短直至自伴侣结构域的Hbp构建体的融合(HbpSS-ESAT6)没有在培养基中产生可检测量的ESAT6。
Hbp、HbpSS、HbpSS-ESAT6、HbpSL(Hbp(Δd1))和HbpSL-ESAT6(又命名为Hbp(Δd1)-ESAT6)的表达和分泌。在新鲜的培养基中继代培养来自过夜培养物的大肠杆菌MC1061细胞(该MC1061细胞携带克隆至表达载体pEH3中的构建体或空载体(泳道1))，并且它们的生长得以继续。当培养物达到早对数期(OD660≈0.3)时，用1mM IPTG诱导Hbp(衍生物)的表达。从诱导后2小时的培养物收集样品，并且通过低速离心将细胞(c)和用过的培养基(m)分离。细胞直接溶解于SDS-PAGE样品缓冲液中，而培养基样品首先进行TCA沉淀。通过SDS-PAGE和考马斯染色分析与0.03OD660单位相对应的细胞样品(A)。通过SDS-PAGE和蛋白质印迹法分析与0.03OD660单位相对应的细胞样品，该蛋白质印迹法使用针对Hbp易位蛋白结构域N端表位的多克隆抗体(B)、针对全长 Hbp载客结构域的多克隆抗体(C)或体针对10kDa结核分枝杆菌蛋白ESAT6(E6)的单克隆抗(D)。在各小图的左侧展示分子质量(kDa)标记。显示了构建体的经加工的载客结构域(>)、经加工的易位蛋白结构域(#)和包含载客结构域及易位蛋白结构域的未加工的原形式(*)。
实例3
携带异源蛋白质的Hbp展示构建体的表达和生物生成(图14)。
本实例显示，在Hbp衍生物(其在载客结构域和易位蛋白结构域之间携带遭破坏的切割位点)中的结构域1(HbpDL-ESAT6)或结构域2(HbpDD2-ESAT6)位置处与Hbp载客结构域融合时，异源蛋白质ESAT6稳定表达。
Hbp(Δβ-cleav)、HbpDL(HbpD(Δd1))、HbpDL-ESAT6(Hbp(Δd1)-ESAT6)、HbpDD2(HbpD(Δd2)和HbpDD2-ESAT6(HbpD(Δd2)-ESAT6)的表达和分泌。在新鲜的培养基中继代培养来自过夜培养物的大肠杆菌MC1061细胞(该MC1061细胞携带克隆至表达载体pEH3中的构建体)，并且它们的生长得以继续。当培养物达到早对数期(OD660≈0.3)时，用1mM IPTG诱导Hbp-衍生物的表达。在2小时诱导后，通过低速离心收集细胞并且使其溶解于SDS-PAGE样品缓冲液中。通过SDS-PAGE和考马斯染色分析与0.03OD660单位相对应的细胞样品(A)。通过SDS-PAGE和蛋白质印迹法分析与0.03OD660单位相对应的细胞样品，该蛋白质印迹法使用针对Hbp易位蛋白结构域N端表位的多克隆抗体(B)、针对全长Hbp载客结构域的多克隆抗体(C)或体针对10kDa结核分枝杆菌蛋白ESAT6(E6)的单克隆抗(D)。在各小图的左侧展示分子质量(kDa)标记。显示了构建体的包含载客结构域和易位蛋白结构域的未加工的原形式(*)。
实例4
在细胞表面展示的Hbp-ESAT6融合物的蛋白酶K可及性(图15)。
本实例显示，携带ESAT6的HbpDL和HbpDD2的载客结构域是添加至完整细胞的蛋白酶K可及的并且因此被蛋白酶K降解，这表明它们暴露于细胞表面。
Hbp(Δβ-cleav)、HbpDL(HbpD(Δd1))、HbpDL-ESAT6(HbpD(Δd1)-ESAT6)、HbpDD2(HbpD(Δd2))和HbpDD2-ESAT6(HbpD(Δd2)-ESAT6)的蛋白酶K可及性。在新鲜的培养基中继代培养来自过夜培养物的大肠杆菌MC1061细胞(该MC1061细胞携带克隆至表达载体pEH3中的构建体)，并且它们的生长得以继续。当培养物达到早对数期(OD660≈0.3)时，用1mMIPTG诱导Hbp-衍生物的表达。通过低速离心从诱导后2小时的培养物收集细胞并且重悬于含有1mM CaCl2的50mM Tris-HCl，PH 7.4中。在Hbp(Δβ-cleav)和HbpDL的情况下，使用尖头超声破碎器(Branson Sonifier 250)通过超声波处理在冰上裂解一半细胞。随后，全部样品与蛋白酶K(pk)(100μg/mL)在37℃孵育1小时。通过添加0.1mM苯甲基磺酰氟(PMSF)并且在冰上孵育5分钟终止反应。在溶解于SDS-PAGE样品缓冲液中之前，将样品进行TCA沉淀。为检测完整细胞上展示的Hbp构建体对于蛋白酶K的可及性，通过SDS-PAGE和考马斯染色分析与0.03OD660单位相对应的细胞样品(A)。作为对照样品，与0.03OD660单位相对应的细胞样品通过SDS-PAGE和蛋白质印迹法分析，该蛋白质印迹法使用针对外膜蛋白OmpA的多克隆抗体，其中该外膜蛋白OmpA是蛋白酶K天然不可及的，除非细胞由例如超声波处理(son)裂解(B)。显示了蛋白酶K处理时出现的OmpA降解产物(x)。在各小图的左侧展示分子质量(kDa)标记。显示了构建体的包含载客结构域和易位蛋白结构域的未加工的原形式(*)。在小图的右手侧显示蛋白酶K(pk)的位置。
实例5.
ESAT6在细胞表面的展示(图16)。
本实例显示，与HbpDL或HbpDD2的载客结构域融合的异源蛋白 质ESAT6是向完整细胞添加的特异性抗体可及的，从而表明ESAT6在细胞上的高效展示。
Hbp(Δβ-cleav)、HbpDL(HbpD(Δd1))、HbpDL-ESAT6(HbpD(Δd1)-ESAT6)、HbpDD2(HbpD(Δd2))和HbpDD2-ESAT6(HbpD(Δd2)-ESAT6)的表面展示分析和无分泌能力的Hbp(ssTorA)和Hbp(OMPLA)。Hbp(ssTorA)是Hbp的突变体，该突变体的天然信号肽由蛋白质TorA的信号肽替换。因为TorA信号肽不将Hbp靶向Sec易位子，没有跨内膜易位发生并且Hbp保留在细胞质中。Hbp(OMPLA)是Hbp的突变体，该突变体的天然易位蛋白结构域由外膜蛋白OMPLA替换。OMPLA的确将Hbp载客结构域靶向外膜，但是不介导其跨外膜易位。因此，Hbp载客结构域保持指向周质并且不指向胞外环境。
在新鲜的培养基中继代培养来自过夜培养物的大肠杆菌MC1061细胞(该MC1061细胞携带克隆至表达载体pEH3中的构建体或空载体(EV))，并且它们的生长得以继续。当培养物达到早对数期(OD660≈0.3)时，用1mM IPTG诱导Hbp-衍生物的表达。在诱导后1小时通过低速离心收集细胞，将其在冰冷的50mM Tris-HCl，PH 7.4中洗涤，并且最终重悬于冰冷的50mM Tris-HCl，PH 7.4中并留在冰上。将每份样品的一半在冰上进行尖端超声波处理(Branson Sonifier 250)以裂解细胞，而另一半细胞保持完整。随后，在冰冷的50mM Tris-HCl，PH 7.4中制备每份样品的5倍稀释物系列。使用基于真空多支管的Bio-Dot设备(Biorad公司)，将每份样品的稀释物施加在预浸泡的硝酸纤维素膜上。在TBS中的5％脱脂乳溶液内孵育20分钟时，将膜封闭。为检测Hbp-衍生物的载客结构域的表面暴露，将膜在TBS中与针对Hbp载客结构域的兔多克隆抗体孵育1小时，用TBS洗涤3次，在TBS中与HRP缀合的山羊抗兔抗体孵育45分钟，用TBS洗涤3次并且使用琥珀磺酸二辛钠(DONS)染色法显影(A)。这证实Hbp(Δβ-cleav)、HbpDL、HbpDL-ESAT6、HbpDD2和HbpDD2-ESAT6的载客结构域在完整细胞上的表面暴露，与不能分泌的突变体Hbp(ssTorA)和Hbp(OMPLA)的载客结构域相反，其中从超声处理的相应样品中显而易见所述突变体的表达。
为了显示ESAT6由HbpDL-ESAT6和HbpDD2-ESAT6在完整细胞上的展示，遵循与A下相同的方法，例外在于使用针对ESAT6的小鼠单克隆抗体和HRP缀合的兔抗小鼠抗体(B)。作为对照，显示不能在完整细胞上高效地检测到周质蛋白OppA，与超声处理的样品相反。对于这种情况，使用与A下所述的相同方法，例外在于使用针对OppA的兔多克隆抗血清(C)。在小图的左手侧处，指示所施加的材料的量(OD660单位)。
实例6
当(部分)缺失侧面结构域时Hbp的生物生成(图17)
本实例显示当侧面结构域1至5的任一个由柔性氨基酸接头序列(Δd1-Δd5)替换时Hbp的成功分泌。另外，显示了成功分泌插入突变体(d4ins)，其中结构域4的仅4个氨基酸由柔性接头替换。
Hbp、Hbp(Δd1)、Hbp(Δd2)、Hbp(Δd3)、Hbp(Δd4)、Hbp(Δd5)和Hbp(d4ins)的表达和分泌。在新鲜的培养基中继代培养来自过夜培养物的大肠杆菌MC1061细胞(该MC1061细胞携带克隆至表达载体pEH3中的构建体或空载体(-))，并且它们的生长得以继续。当培养物达到早对数期(OD660≈0.3)时，用1mM IPTG诱导Hbp(衍生物)的表达。从诱导后2小时的培养物收集样品，并且通过低速离心将细胞(c)和用过的培养基(m)分离。细胞直接溶解于SDS-PAGE样品缓冲液中，而培养基样品首先进行TCA沉淀。通过SDS-PAGE和考马斯染色分析与0.03OD660单位相对应的细胞样品。
正确的分泌是从切割的载客结构域(>)在细胞部分(c)和培养基(m)中以及切割的易位蛋白结构域(β)在细胞部分中出现而得出的，与野生型Hbp(wt％)相似(图17)。在小图的左侧显示分子质量(kDa)标记。
实例7
与结构域d1至d5中任一个的位置融合的ESAT6的分泌(图18)
本实例显示与Hbp载客结构域在结构域d1至d5中任一个的位置处融合或插入结构域4(d4ins)时结核分枝杆菌抗原ESAT的高效分泌。
Hbp(Δd1)-ESAT6、Hbp(Δd2)-ESAT6、Hbp(Δd3)-ESAT6、Hbp(Δd4)-ESAT6、Hbp(Δd5)-ESAT6和Hbp(d4ins)-ESAT6的表达和分泌。(A)如实例6下所描述那样培育、诱导和分析大肠杆菌MC1061细胞，该MC1061细胞携带克隆至表达载体pEH3中的构建体。(B)使用针对ESAT6的单克隆抗体，通过蛋白质印迹法分析来自A的与0.003OD660单位相对应的细胞样品。
正确的分泌是从切割的载客结构域(>)在细胞部分(c)和培养基(m)中以及切割的易位蛋白结构域(β)在细胞部分中出现而得出的(图18A)。使用ESAT6特异性抗体，通过蛋白质印迹法证实ESAT6在相应的载客结构域中的存在(图18B)。在各小图的左侧展示分子质量(kDa)标记。
实例8
当替换结构域d1或d2时分泌TB10.3和TB10.4(图19)
本实例显示当分别替换Hbp载客结构域的结构域d2和结构域d1时结核分枝杆菌蛋白TB10.3和TB10.4的高效分泌。
Hbp(Δd1)-TB10.4和Hbp(Δd2)-TB10.3的表达和分泌。如描实例6下所描述那样培育和诱导大肠杆菌TOP10F’细胞，该TOP10F’细胞携带克隆至表达载体pEH3中的构建体或携带非表达性质粒(-)。从诱导后2小时的培养物抽取样品。随后，通过离心分离细胞，将其溶解于SDS-PAGE样品缓冲液中并且通过考马斯染色的SDS-PAGE进行分析。
正确的分泌是从切割的载客结构域(>)在细胞部分(c)和培养基(m)中以及切割的易位蛋白结构域(β)在细胞部分中出现而得出的(图19)。在小图的左侧显示分子质量(kDa)标记。
实例9
与结构域d3、d4或d5中任一个的位置融合的Rv2660c的分泌(图20)
本实例显示与Hbp载客结构域在结构域3、结构域4或结构域5的位置处融合时结核分枝杆菌抗原Rv2660c的高效分泌。
Hbp(Δd3)/Rv2660c、Hbp(Δd4)/Rv2660c、Hbp(Δd5)/Rv2660c的表达和分泌。如实例6下所描述那样培育、诱导和分析大肠杆菌MC1061细胞，该MC1061细胞携带克隆至表达载体pEH3中的构建体。
正确的分泌是从切割的载客结构域(>)在细胞部分(c)和培养基(m)中以及切割的易位蛋白结构域(β)在细胞部分中出现而得出的(图20)。在小图的左侧显示分子质量(kDa)标记。
实例10
无半胱氨酸的hEGF的分泌(图21)。
本实例显示与Hbp载客结构域在结构域1的位置处融合时智人蛋白质hEGF(EGF)的无半胱氨酸(0ss)形式的高效分泌。
Hbp(野生型)、Hbp(Δd1)和Hbp(Δd1)-hEGF(0ss)的表达和分泌。将大肠杆菌MC1061细胞在补充有葡萄糖(0.4％)、氯霉素(20μg/mL)和链霉素(30μg/mL)的M9培养基中在37℃培育过夜，其中该MC1061细胞携带克隆至表达载体pEH3中的构建体。次日早晨，在新鲜培养基中继代培养细胞并且它们的生长得以继续。当培养物达到早对数期(OD660≈0.3)时，用1mM IPTG诱导Hbp(衍生物)的表达。从诱导后3小时的培养物收集样品，并且通过低速离心将细胞(c)和用过的培养基(m)分离。细胞直接溶解于SDS-PAGE样品缓冲液中，而培养基样品首先进行TCA沉淀。通过SDS-PAGE和考马斯染色分析与0.05OD660单位相对应的细胞样品和与0.1OD660单位相对应的培养基样品。
正确的分泌是从切割的载客结构域(>)在细胞部分(c)和培养基(m)中以及切割的易位蛋白结构域(β)在细胞部分中出现而得出的(图21)。在小图的左侧显示分子质量(kDa)标记。
实例11
插入β-茎部形成序列时ESAT6的受损的分泌(图22)
本实例显示ESAT6在Hbp载客结构域的β-茎部形成序列中的插入(βins-ESAT6)导致与ESAT6替换侧面结构域4(Δd4-ESAT6)相比的低效分泌。值得注意的是，相应构建体中的ESAT6融合位点仅相隔5个氨基酸残基。
Hbp(野生型)、Hbp(Δd4)-ESAT6和Hbp(βins)-ESAT6的表达和分泌。如实例6下所描述那样培育和诱导大肠杆菌MC1061细胞，该MC1061细胞携带克隆至表达载体pEH3中的构建体。从诱导后2小时的培养物抽取样品。随后，通过离心分离细胞，将其溶解于SDS-PAGE样品缓冲液中并且通过考马斯染色的SDS-PAGE进行分析。在各小图的左侧展示分子质量(kDa)标记。显示切割的Hbp载客结构域种类(>)。
实例12
与替代性自转运蛋白融合时ESAT6的分泌(图23-24)
本实例显示了用来通过Hbp分泌系统分泌异源蛋白质的方法论适用于替代性自转运蛋白(AT)；EspC。
图23A显示EspC载客结构域结构的模型。使用M4T同源性建模方法(Rykunov等人，2009J Struct Funct Genomics(《结构与功能基因组学杂志》)10：95-99)，以计算机方式预测该结构。使用与EspC载客结构域(登录号Q9EZE7的蛋白质的残基54-1028)相对应的一级氨基酸序列作为输入。鉴定到从β茎部突出的侧面结构域。结构域1(d1)为浅灰色。载客结构域的其余部分(包括β茎部结构域)着色为黑色。结构域d1 适合由POI替换。
图23B显示这些实例中所用的EspC衍生物的示意图。将EspC(野生型)作为1306个氨基酸(aa)的前体合成，该前体以3个结构域构成：(i)N端可切割的信号序列(ss；aa 1-53)，(ii)载客结构域(aa 54-1028)和(iii)外膜整合的C端易位蛋白结构域(β-结构域；aa1029-1306)。显示预测的结构域1(d1)。载客结构域的其余部分(包括β茎部结构域)着色为黑色。“FL”指柔性接头。“E-6”表示ESAT6。在通过外膜后，载客结构域借助自催化机制被从易位蛋白结构域(β-结构域)切下，该自催化机制涉及水解EspC前体的Asn1028和Asn1029之间的肽键。上文示意图中所示的数字对应于原始EspC(野生型)前体的氨基酸位置，从N端算起。
EspC、EspC(Δd1)和EspC(Δd1)-ESAT6的表达和分泌。(图24A)如实例6下所描述那样培育、诱导和分析大肠杆菌MC1061细胞，该MC1061细胞携带克隆至表达载体pEH3中的构建体。(图24B)使用针对ESAT6的单克隆抗体，通过蛋白质印迹法分析来自A的与0.003OD660单位相对应的细胞样品。
本文显示，当载客结构域的预测的dom1由柔性氨基酸序列(Δd1)替换时，EspC载客结构域的分泌按照与野生型EspC(wt)相同的效率推进。另外，本实例显示，与EspC载客结构域在预测的结构域d1的位置处融合时(Δd1/ESAT6)，ESAT6可以高效地分泌。正确的分泌是从切割的载客结构域(>)在培养基部分(m)中和切割的易位蛋白结构域在细胞部分中(x)出现而得出的(图24A)。使用ESAT6特异性抗体，通过蛋白质印迹法证实ESAT6在EspC(d1/ESAT6)载客结构域中存在(图24B)。在各小图的左侧展示分子质量(kDa)标记。
实例13
分裂的Ag85B和ESAT6的分泌(图25)
本实例显示同时分泌分别大致与结核分枝杆菌抗原Ag85B的成熟 区域的N’端和C’端半侧相对应的氨基酸段Ag85B[N’]和Ag85B[C’]。当Ag85B[N’]在结构域1的位置处并且Ag85B[C’]在相同Hbp载客结构域分子的结构域2的位置处与Hbp载客结构域翻译性融合(85[N+C])时，实现这两个部分的分泌。可替代地，Ag85B[C’]在结构域1的位置融合并且Ag85B[N’]在结构域2的位置融合(85[N+C])。
此外，本实例显示Ag85B[N’]、Ag85B[C’]和分支杆菌抗原ESAT6的同时分泌。当在相同Hbp载客结构域分子上Ag85B[N’]在结构域1的位置处、Ag85B[C’]在结构域2的位置处融合并且ESAT6插入结构域4(85[N+C]/E6)时，实现这种分泌。可替代地，Ag85B[C’]在结构域1的位置融合，Ag85B[N’]在结构域2的位置融合，并且ESAT6插入结构域4中(85[N+C]/E6)。
Hbp、Hbp(Δβ-cleav)、Hbp-Ag85B[N+C]、Hbp-Ag85B[C+N]、Hbp-Ag85B[N+C]/ESAT6、Hbp-Ag85B[C+N]/ESAT6的表达、分泌和蛋白酶K可及性。(图25A)如实例6下所描述那样培育、诱导和分析来自过夜培养物的大肠杆菌TOP10F’细胞，该MC1061细胞携带克隆至表达载体pEH3中的构建体或空载体(-)。(图25B)如A下所培养和诱导的细胞重悬于含有1mM CaCl2的50mMTris-HCl，PH 7.4中。随后，将样品于37℃在存在(+)或不存在(-)蛋白酶K(pk)(100μg/mL)的情况下孵育1小时。通过添加0.1mM苯甲基磺酰氟(PMSF)并且在冰上孵育5分钟终止反应。在溶解于SDS-PAGE样品缓冲液中之前，将样品进行TCA沉淀并且在考马斯染色的SDS-PAGE上分析。在各小图的左侧展示分子质量(kDa)标记。显示切割的载客结构域(>)和易位蛋白结构域(β)、蛋白酶K(pk)的位置。
正确的分泌是从切割的载客结构域(>)和易位蛋白结构域(β)在细胞部分(c)中出现而得出的，与野生型Hbp相似(A)。作为对照，对不可切割但是能够易位的Hbp(Δβ)形式，没有观察到切割的载客结构域和易位蛋白结构域。为证实它们的胞外位置，显示了这些载客结构域对添加至完整细胞的蛋白酶K的敏感性(B)。
实例14
分裂的Ag85B、ESAT6和Rv2660的同时分泌(图26)
本实例显示Ag85B[N’]、Ag85B[C’]、ESAT6和分支杆菌抗原Rv2660c与单个Hbp载客结构域融合时同时分泌。当Ag85B[N’]在结构域1的位置处与Hbp载客结构域翻译性融合、Ag85B[C’]在结构域2的位置处融合、ESAT6插入结构域4，并且Rv2660c在结构域5的位置处融合时(“85-E6-2660”)，实现这些部分的分泌。为了比较，平行分析仅携带Ag85B[C’]及Ag85B[N’](“85”)、或Ag85B[C’]、Ag85B[N’]和ESAT6(“85-E6”)的构建体的分泌。
Hbp-Ag85B[C+N]，Hbp-Ag85B[C+N]/ESAT6和Hbp-Ag85B[C+N]/ESAT6/Rv2660c的表达和分泌。如实例6下所描述那样培育和诱导大肠杆菌MC1061细胞，该MC1061细胞携带克隆至表达载体pEH3中的构建体。
正确的分泌是从切割的载客结构域(>)和易位蛋白结构域(x)在细胞部分(c)中出现而得出的(图26)。在小图的左侧显示分子质量(kDa)标记。
实例15
Ag85B、ESAT6和Rv2660c的同时展示(图27)
本实例显示Ag85B[N’]、Ag85B[C’]、ESAT6和Rv2660c与Hbp(Δβcleav)(一种不可切割然而能够易位的Hbp形式)的单个载客结构域融合时同时展示。当Ag85B[N’]在结构域1的位置处与Hbp载客结构域翻译性融合、Ag85B[C’]在结构域2的位置处融合、ESAT6插入结构域4，并且Rv2660c在结构域5的位置处融合时(85-E6-2660)，实现展示。为了比较，平行分析仅携带Ag85B[C’]及Ag85B[N’](“85”)、或Ag85B[C’]、Ag85B[N’]和ESAT6(“85-E6”)的构建体的展示。另外，分析Hbp(Δβcleav)(Δβ)的展示(图27A)。
在适宜的情况下，使用针对Ag85B[C’]、ESAT6和Rv2660c的特异性抗体实施蛋白质印迹以证实这些部分在相应的载客结构域中的存在(图27B)。
为证实相应的载客结构域跨细胞包膜易位和它们在细胞表面展示，显示了这些载客结构域对添加至完整细胞的蛋白酶K的敏感性(图27C)。
在图27中，在各小图的左侧展示分子质量(kDa)标记。显示了未切割的原形形式Hbp种类(*)。
Hbp(Δβcleav)、HbpD-Ag85B[C+N]、HbpD-Ag85B[C+N]/ESAT6和HbpD-Ag85B[C+N]/ESAT6/Rv2660c的表达和展示。(A)如实例6下所描述那样培育和诱导大肠杆菌MC1061细胞，该MC1061细胞携带克隆至表达载体pEH3中的构建体。从诱导后2小时的培养物抽取样品。随后，通过离心分离细胞，将其溶解于SDS-PAGE样品缓冲液中并且通过考马斯染色的SDS-PAGE进行分析。(B)使用针对Ag85B[C]的表位的单克隆抗体、针对ESAT6的单克隆抗体或针对Rv2660c的多克隆抗体，通过蛋白质印迹法分析来自A的与0.003OD660单位相对应的细胞样品。(C)如A下所培养和诱导的细胞重悬于含有1mM CaCl2的50mM Tris-HCl，PH 7.4中。随后，将样品于0℃在存在(+)或不存在(-)蛋白酶K(pk)(100μg/mL)的情况下孵育30分钟。通过添加0.1mM苯甲基磺酰氟(PMSF)并且在冰上孵育5分钟终止反应。在溶解于SDS-PAGE样品缓冲液中并且在考马斯染色的SDS-PAGE上分析之前，将样品进行TCA沉淀。
实例16
Ag85B、ESAT6和Rv2660c由减毒的沙门氏菌菌株同时分泌(图28)
本实例显示与单个Hbp载客结构域融合时，Ag85B[N’]、Ag85B[C’]、ESAT6和分支杆菌抗原Rv2660c由减毒的鼠伤寒沙门氏菌(Salmonella typhimurium)同时分泌。当Ag85B[C’]在结构域1的位置处 与Hbp载客结构域翻译性融合、Ag85B[N’]在结构域2的位置处融合、ESAT6插入结构域4，并且Rv2660c在结构域5的位置处融合时(85[C+N]-E6-2660)，实现这些部分的分泌。正确的分泌是从切割的载客结构域(>)在培养基部分(m)中出现而得出的(图28A)。
使用针对Ag85B[C’]、ESAT6和Rv2660c的特异性抗体实施蛋白质印迹以证实这些部分在分泌的载客结构域中存在。另外，使用针对Hbp易位蛋白结构域(α桶)的抗血清的蛋白质印迹法证实切割的易位蛋白结构域在细胞中出现(图28B)。
由减毒的鼠伤寒沙门氏菌表达和分泌Hbp-Ag85B[C+N]-ESAT6-Rv2660c。(A)将鼠伤寒沙门氏菌菌株SL3261(Hoiseth和Stocker 1981Nature(《自然》)291：281-282)和衍生物在LB培养基中于37℃过夜培育至饱和，其中该衍生物在基因组上携带在组成型lacUV5启动子控制下编码Hbp-Ag85B[C+N]-ESAT6-Rv2660c的基因的单拷贝。对于沙门氏菌菌株的构建，见实例21。次日早晨，在新鲜培养基中继代培养细胞并且它们的生长得以继续。继代培养后2小时，从培养物收集样品，并且通过低速离心将细胞(c)和用过的培养基(m)分离。细胞直接溶解于SDS-PAGE样品缓冲液中，而培养基样品首先进行TCA沉淀。通过SDS-PAGE和考马斯染色分析与0.03OD660单位相对应的细胞样品。(B)使用针对Ag85B[C]的表位的单克隆抗体、针对ESAT6的单克隆抗体、针对Rv2660c的多克隆抗体或针对Hbp易位蛋白结构域的多克隆抗体，通过蛋白质印迹法分析来自A的与0.003OD660单位相对应的细胞样品。
显示了一条身份未知的背景条带(*)。在各小图的左侧展示分子质量(kDa)标记。
实例17
Ag85B、ESAT6和Rv2660c由减毒的沙门氏菌菌株同时展示(图29)
本实例显示，与Hbp(Δβcleav)(一种不可切割然而能够易位的 Hbp形式(HbpD-Ag85B[C+N]-ESAT6-Rv2660c))的载客结构域的单个Hbp载客结构域融合时，Ag85B[N’]、Ag85B[C’]、ESAT6和Rv2660c在减毒的鼠伤寒沙门氏菌的细胞表面表达并同时展示。还显示使用相同策略时单个ESAT6单元的表面展示(HbpD-ESAT6)。
构建体的正确表达是通过SDS-PAGE和考马斯染色分析而得出的，该分析显示分子量分别与HbpD-Ag85B[C+N]-ESAT6-Rv2660c和HbpD-ESAT6的计算分子量相对应的蛋白质条带出现(*)。作为对照，这些条带不存在于不表达的对照细胞(-)中。
为证实相应的载客结构域跨细胞包膜的易位和在细胞表面展示，显示了它们对添加至完整细胞的蛋白酶K(pk)的敏感性。
HbpD-Ag85B[C+N]-ESAT6-Rv2660c和HbpD-ESAT6在减毒的鼠伤寒沙门氏菌的细胞表面上的表达和展示。将鼠伤寒沙门氏菌菌株SL3261(Hoiseth和Stocker 1981Nature(《自然》)291：281-282)(-)和衍生物在LB培养基中于37℃过夜培育至饱和，该衍生物在基因组上携带在组成型lacUV5启动子控制下编码HbpD-Ag85B[C+N]-ESAT6-Rv2660c的基因的或编码HbpD-ESAT6的基因的单拷贝。对于沙门氏菌菌株的构建，见实例21。次日早晨，在新鲜培养基中继代培养细胞并且它们的生长得以继续。继代培养后2小时30分钟，从培养物收集细胞并且重悬于含有1mMCaCl2的50mM Tris-HCl，PH 7.4中。随后，将样品于37℃在存在(+)或不存在(-)蛋白酶K(pk)(100μg/mL)的情况下孵育1小时。通过添加0.1mM苯甲基磺酰氟(PMSF)并且在冰上孵育5分钟终止反应。将样品进行TCA沉淀，溶解于SDS-PAGE样品缓冲液中，并且在考马斯染色的SDS-PAGE上分析。显示了构建体的未加工的原形式(*)，其包含载客结构域和易位蛋白结构域。在小图的右手侧显示分子量标记(kDa)。
实例18
Ag85B、ESAT6和Rv2660c在外膜小泡上同时展示(图30A-D)。
这份样品显示Ag85B[C’]、Ag85B[N’]和ESAT6与Hbp(Δβcleav) (一种不可切割然而能够易位的Hbp形式(85B-E6-2660))的单个载客结构域融合时同时展示在细菌外膜小泡(OMV)的表面上。此外，显示了Ag85B[C’]、Ag85B[N’]和ESAT6(85B-E6)以及单个ESAT6单元(Δd1-E6)的组合展示。作为对照，分析了不携带异源配偶物(Δd1)的HbpD(Δd1)的展示。为实现展示在OMV上，将这些融合蛋白在tol-pal基因中携带突变的大肠杆菌菌株内表达，其中该突变诱导过度小泡化(hyper-vesiculating)表型。
这些融合蛋白在OMV中的定位由它们与外膜孔蛋白(porin)OmpA和OmpC在OMV分离物中的共定位显示，其中该OMV分离物源自经过滤并且因此无细胞的培养基部分(图30A和B)。这些融合蛋白在OMV表面的成功展示由它们对外在添加至OMV的蛋白酶K的敏感性显示(图30C和D)。为证实OMV的完整性，显示蛋白酶K敏感的OmpA胞内结构域是不可及的，除非使用去垢剂曲拉通x-100(tx-100)溶解OMV。
HbpD(Δd1)、HbpD(Δd1)-ESAT6、HbpD-Ag85B[C+N]-ESAT6和Ag85B[C+N]-ESAT6-Rv2660c在OMV上的表达和展示。(A)在新鲜的培养基中继代培养来自过夜培养物的大肠杆菌JC8031细胞(Barnadac等人，1998Journal of Bacteriology(《细菌学杂志》)180：4872-4878)，该JC8031细胞携带克隆至表达载体pEH3中的构建体或空载体(-)，并且它们的生长得以继续。当培养物达到早对数期(OD660≈0.2)时，用1mM IPTG诱导Hbp(衍生物)的表达。在诱导后3小时，将50ml培养物样品离心(5000转/分钟，4℃，15分钟)以将细胞与培养基分离。将细胞(总细胞)溶解于SDS-PAGE样品缓冲液中，而将培养基经历再一次离心(5000转/分钟，4℃，15分钟)。将所得到的上清液经0.2μm孔径滤器过滤并且使用Kontron TFT 70.38转子，进行高速离心(45,000转/分钟，4℃，1小时)。含有OMV的沉淀部分重悬于PBS中。将与1OD660单位相对应的细胞样品溶解于SDS-PAGE样品缓冲液中并且通过考马斯染色的SDS-PAGE与0.02OD660单位的总细胞进行平行分析。已经在小图左侧指示外膜蛋白OmpA和OmpC，其身份通过质谱分析证实。(B)为证实融合蛋白的身份，使用针对Hbp易位蛋白结构域(α桶)的多克隆抗血清和针对ESAT6的单 克隆抗体，通过蛋白质印迹法分析在A下制备的样品。(C)OMV的蛋白酶K处理。在A下所分离的OMV重悬于含有1mM CaCl2的50mM Tris-HCl，PH 7.4中。将样品分成3个相等的等分试样，这些样品如所示那样与(+)或不与(-)蛋白酶K(pk)(100μg/ml)孵育。在添加蛋白酶K之前，将曲拉通X-100(tx-100)(1％)添加至等分试样之一。全部等分试样在37℃孵育30分钟，此后，通过添加0.1mM苯甲基磺酰氟(PMSF)并且在冰上孵育5分钟终止反应。将样品进行TCA沉淀，溶解于SDS-PAGE样品缓冲液中。与1OD660单位相对应的细胞样品由考马斯染色的SDS-PAGE分析。(D)为证实融合蛋白的身份，使用针对Hbp易位蛋白结构域(α桶)的多克隆抗血清和针对ESAT6的单克隆抗体，通过蛋白质印迹法分析在C下制备的样品。显示了构建体的未加工的原形式(*)，其包含载客结构域和易位蛋白结构域。在小图的右手侧显示分子量标记(kDa)。
实例19
分裂的Ag85B和ESAT6在菌影上的展示(图31A-C)
在本实例19中，使用质粒pLargeRhaLysisE来利用本文中所述的方法学，将大肠杆菌细胞变成菌影。在实例20中描述pLargeRhaLysisE的构建。
这份样品显示Ag85B[C]、Ag85B[N]和ESAT6与Hbp(Δβcleav)的单个载客结构域融合时同时展示在菌影的表面上。为了实现在菌影上表面展示，在用质粒转化的大肠杆菌细胞中表达HbpD-Ag85B[C+N]-ESAT6，其中该质粒携带在诱导型启动子的控制下编码噬菌体phiX174裂解蛋白E的基因。在表达HbpD-Ag85B[C+N]-ESAT6后，诱导裂解蛋白E的表达，导致细胞胞质内容物释放至培养基中。这导致出现在表面展示HbpD-Ag85B[C+N]-ESAT6的‘空’细菌细胞包膜(菌影)。
通过裂解蛋白E表达时表观细胞密度(OD600)的降低显示成功的裂解蛋白E介导的菌影形成(图31A)。另外，显示裂解蛋白E表达时，胞质标记蛋白(SecB,DnaK)释放至培养基中并且因此在离心后留在上清 液部分中。相反，周质标记蛋白(SurA)和整合型膜蛋白(Lep)(主要)位于含有细菌细胞包膜(菌影)的离心沉淀物中(图31B)。HbpD-Ag85B[C+N]-ESAT6在菌影中的正确定位由它与沉淀物部分中的Lep共定位显示(图31B)。HbpD-Ag85B[C+N]-ESAT6在菌影以及对照细胞表面的成功展示由它对外在添加至这些菌影/细胞的蛋白酶K的敏感性显示(图31C)。
]HbpD-Ag85B[C+N]-ESAT6的表达和展示及菌影的后续形成。(A)用(i)pEH3(p15a)-HbpD-Ag85B[C+N]-ESAT6和(ii)携带下述基因的pLargeRhaLysisE共转化的大肠杆菌MC4100细胞在LB培养基中于30℃培育，其中该基因在鼠李糖诱导型启动子的控制下的、编码来自噬菌体phiX174的裂解蛋白E。当培养物达到OD600为0.5时，添加0.4mM IPTG以诱导HbpD-Ag85B[C+N]-ESAT6的表达，并且将培养物分开。在添加IPTG后1小时，将0.2％鼠李糖添加至一半的原始培养物以诱导裂解蛋白E的表达，而使用另一半培养物作为对照。随时间推移监测培养物的OD600。(B)在IPTG诱导后3小时，通过离心分离在A下所培育的对照细胞(未诱导的培养物)和菌影(诱导的培养物)。分离含有培养基的上清并且用TCA沉淀其蛋白质内容物。细胞/菌影沉淀物(P；细胞/菌影沉淀物)和TCA沉淀的物质(S；上清液)然后溶解于SDS-PAGE样品缓冲液中并且由SDS-PAGE和蛋白质印迹法分析。使用针对Hbp载客结构域(pass.)和易位蛋白结构域(桶)的多克隆抗体和针对ESAT6的单克隆抗体检测HbpD-Ag85B[C+N]-ESAT6的存在。使用针对胞质蛋白DnaK和SecB、周质蛋白SurA和整合型膜蛋白Lep的多克隆抗体监测菌影的形成。(C)对照细胞和菌影的蛋白酶K处理。在B下所分离的细胞和菌影的部分重悬于含有1mM CaCl2的50mM Tris-HCl，PH 7.4中。将蛋白酶K(100μg/ml)添加至每份样品的一半(+)，而留下另一半不作处理(-)。样品在37℃孵育1小时，此后，通过添加0.1mM苯甲基磺酰氟(PMSF)并且在冰上孵育5分钟终止反应。将样品进行TCA沉淀，溶解于SDS-PAGE样品缓冲液中并且使用针对Hbp载客结构域的抗血清，通过蛋白质印迹法进行分析。
实例20
以下实例涉及质粒pLargeRhaLysisE的构建。图32显示实例20中所用的pLargeRhaLysisE的质粒图。下表3和表4分别显示实例20中使用的引物序列和构建体。
质粒pLargeRhaLysisE携带在鼠李糖诱导型rhaBAD启动子控制下的、编码来自噬菌体phiX174的裂解蛋白E的基因。为构建pLargeRhaLysisE，首先构建了基于pSB3398(Wagner等人，2010Proc Natl Acad Sci USA(《美国科学院院刊》)107:17745-17750)和pRha67K的质粒pLarge。
质粒pRha67K是pRha67的衍生物(Giacalone等人，2006BioTechniques(《生物学技术》)40：355-364)，其中使用USER克隆法(Bitinaite和Nichols 2009Curr Protoc MolBiol(《分子生物学现行方案》)第3章：3.21单元；2010BMC Biotechnol 10：21)，将编码氨苄青霉素标记的基因从起始至终止密码子用编码来自pET28(a+)(EMDBiosciences公司)的卡那霉素标记的基因替换。使用pET28(a+)作为模板以及含有脱氧尿嘧啶(u)的引物kanR和kanF，扩增编码pET28(a+)的卡那霉素抗性标记的基因。使用含有脱氧尿嘧啶的引物pRhakanF和pRhakanR，扩增了编码pRha67的DNA，该pRha67不带编码氨苄青霉素标记的基因。使用含有脱氧尿嘧啶的引物，将PfuX7聚合酶用来扩增DNA(2010BMC Biotechnol 10：21)。随后，根据制造商的说明书，使用USER酶(NewEngland Biolabs公司)构建pRha67K。
质粒pLarge是pRha67K的衍生物，其中使用USER克隆法(Bitinaite和Nichols2009Curr Protoc Mol Biol(《分子生物学现行方案》)第3章：3.21单元；2010BMCBiotechnol 10：21)，将鼠李糖启动子(包括调节元件)、多克隆位点和终止子用来自pSB3398的对应物替换(Wagner等人，2010Proc Natl Acad Sci USA(《美国科学院院刊》)107:17745-17750)。使用pSB3398作为模板以及含有脱氧尿嘧啶的引物 pSB3398正向和pSB3398反向，扩增调节元件(rhaR、rhaS PrhaBAD)和多克隆位点和转录终止子rrnB。使用pRha67K作为模板来扩增pRha67K的部分，该部分涵盖卡那霉素抗性标记(包括其启动子和终止子)和复制起点。所用的含有脱氧尿嘧啶的引物是67kF和pRhaR。PfuX7聚合酶用来扩增DNA，并且随后，根据制造商的说明书使用USER酶(New England Biolabs)来构建pLarge。
为构建pLargeRhaLysisE，从MWG公司获得编码来自噬菌体phiX174的裂解蛋白E的合成DNA序列。这种合成DNA片段在编码序列的5’侧和3’侧处分别拥有EcoRI和BamHI位点。这允许克隆至pLarge的EcoRI和BamHI位点，从而产生pLargeRhaLysisE。
表3：实例20中使用的引物
注：在表3中，尿嘧啶用u指示。
表4.实例20中使用的构建体
实例21
以下实例21涉及沙门氏菌菌株的构建。图33显示hbp突变插入鼠伤寒沙门氏菌SL3261染色体中的位置的示意图。下表5显示实例21中所用的引物序列。
如下构建在染色体上携带编码Hbp-Ag85B(C+N)-ESAT6-Rv2660c、HbpD-ESAT6或HbpD-Ag85B(C+N)-ESAT6-Rv2660c的基因中的任一的单拷贝的鼠伤寒沙门氏菌菌株。将相关的hbp突变体基因借助双交换同源重组通过等位交换插入鼠伤寒沙门氏菌染色体(Kaniga等人，1991Gene(《基因》)109：137-141)，替换malE和malK启动子区。简而言之，分别使用pHbp-Ag85B(C+N)-ESAT6-Rv2660c、pHbpD-ESAT6或pHbpD-Ag85B(C+N)-ESAT6-Rv2660c作为模板，通过PCR扩增包含lacUV5启动子区的hbp突变体。所用的引物是lacUV5_ScaI_f和pEH3Hbpbeta_ScaI_r。PCR产物用ScaI消化并克隆至SmaI切割的源自pSB890的自杀载体(Palmer等人，1998Molecular Microbiology(《分子微生物学》)27：953-965)中，就在与malE和malK同源的1000bp区域之间(图33)。
将所得到的hbp突变体-自杀载体转化至大肠杆菌供体菌株SM10λpir(Miller和Mekalanos 1998Journal of Bacteriology(《细菌学杂志》)170：2575-2583)中。SM10λpir在平板上与鼠伤寒沙门氏菌受体菌株SL3261交合过夜(Hoiseth和Stocker 1981Nature(《自然》)291：281-282)。在平板上选择四环素耐受型鼠伤寒沙门氏菌转化接合子。
通过选择沙门氏菌突变体针对蔗糖的抗性实现部分二倍体的解析和用hbp突变体基因替换野生型基因座(Kaniga等人，1991Gene(《基因》)109：137-141)。使用引物malE_insert_seq和malK_insert_seq，通过malE和malK之间基因间区的PCR并将导入的等位基因测序，鉴定阳性克隆。
表5.实例21中使用的引物
序列表
<110>Xbrane Bioscience AB
<120>用于分泌性蛋白表达的融合蛋白
<130>IPQ2754
<150>SE 1051000-6
<151>2010-09-28
<160>137
<170>PatentIn 版本3.5
<210>1
<211>1377
<212>PRT
<213>大肠杆菌
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Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
660 665 670
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
675 680 685
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
690 695 700
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
705 710 715 720
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser
725 730 735
Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu
740 745 750
Glu Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe
755 760 765
Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp
770 775 780
Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser
785 790 795 800
Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu
805 810 815
Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser
820 825 830
Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg
835 840 845
Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly
850 855 860
Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr
865 870 875 880
Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr
885 890 895
Leu Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln
900 905 910
Met Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser
915 920 925
Leu Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser
930 935 940
Met Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile
945 950 955 960
Val Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp
965 970 975
Asn Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn
980 985 990
Lys Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn
995 1000 1005
Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr
1010 1015 1020
Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn
1025 1030 1035
Leu Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr
1040 1045 1050
Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val
1055 1060 1065
Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala
1070 1075 1080
Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu
1085 1090 1095
Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn
1100 1105 1110
Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser
1115 1120 1125
Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly
1130 1135 1140
Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly
1145 1150 1155
Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr
1160 1165 1170
Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly
1175 1180 1185
Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile
1190 1195 1200
His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys
1205 1210 1215
Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly
1220 1225 1230
Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala
1235 1240 1245
Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn
1250 1255 1260
Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro
1265 1270 1275
Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly
1280 1285 1290
Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe
1295 1300 1305
Asp Leu Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly
1310 1315 1320
Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly
1325 1330 1335
Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu
1340 1345 1350
Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala
1355 1360 1365
Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1370 1375
<210>2
<211>1377
<212>PRT
<213>大肠杆菌
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Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
660 665 670
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
675 680 685
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
690 695 700
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
705 710 715 720
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser
725 730 735
Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu
740 745 750
Glu Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe
755 760 765
Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp
770 775 780
Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser
785 790 795 800
Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu
805 810 815
Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser
820 825 830
Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg
835 840 845
Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly
850 855 860
Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr
865 870 875 880
Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr
885 890 895
Leu Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln
900 905 910
Met Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser
915 920 925
Leu Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser
930 935 940
Met Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile
945 950 955 960
Val Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp
965 970 975
Asn Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn
980 985 990
Lys Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn
995 1000 1005
Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr
1010 1015 1020
Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn
1025 1030 1035
Leu Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr
1040 1045 1050
Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val
1055 1060 1065
Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala
1070 1075 1080
Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu
1085 1090 1095
Val Gly Ser Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn
1100 1105 1110
Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser
1115 1120 1125
Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly
1130 1135 1140
Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly
1145 1150 1155
Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr
1160 1165 1170
Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly
1175 1180 1185
Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile
1190 1195 1200
His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys
1205 1210 1215
Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly
1220 1225 1230
Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala
1235 1240 1245
Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn
1250 1255 1260
Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro
1265 1270 1275
Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly
1280 1285 1290
Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe
1295 1300 1305
Asp Leu Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly
1310 1315 1320
Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly
1325 1330 1335
Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu
1340 1345 1350
Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala
1355 1360 1365
Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1370 1375
<210>3
<211>445
<212>PRT
<213>大肠杆菌
<400>3
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Cys Gly Ser Gly Ser Gly Ala Asp Lys
50 55 60
Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val
65 70 75 80
Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu
85 90 95
Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr
100 105 110
Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys
115 120 125
Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg
130 135 140
Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr
145 150 155 160
Asn Asn Phe Ile Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp
165 170 175
Leu Arg Asp Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn
180 185 190
Gly Ser Gly Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu
195 200 205
Gln Met Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe
210 215 220
Thr Gly Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu
225 230 235 240
Tyr Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly
245 250 255
Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His
260 265 270
Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn
275 280 285
Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr
290 295 300
His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp
305 310 315 320
Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met Asp
325 330 335
Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg Thr Gly
340 345 350
Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser Leu Thr Ala
355 360 365
Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp Ser Ala Asp Val
370 375 380
His Leu Lys Asp Ala Ala Gly Glu His Gln Ile Asn Gly Arg Lys Asp
385 390 395 400
Ser Arg Met Leu Tyr Gly Val Gly Leu Asn Ala Arg Phe Gly Asp Asn
405 410 415
Thr Arg Leu Gly Leu Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn
420 425 430
Thr Asp Asp Ala Ile Asn Ala Asn Ile Arg Tyr Ser Phe
435 440 445
<210>4
<211>1131
<212>PRT
<213>大肠杆菌
<400>4
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Cys Gly Ser Gly Ser Gly Asn Asp Ala
50 55 60
Pro Val Thr Phe Arg Thr Ser Glu Gly Gly Ala Leu Glu Trp Ser Phe
65 70 75 80
Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr Gln Gly Thr Thr Thr Tyr
85 90 95
Ala Met His Gly Gln Gln Gly Asn Asp Leu Asn Ala Gly Lys Asn Leu
100 105 110
Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn Leu Lys Asp Ser Val Ser
115 120 125
Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp Asn Tyr Thr Val Thr Thr
130 135 140
Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly Ile Val Val Asp Asn Gly
145 150 155 160
Val Ser Val Asn Trp Gln Val Asn Gly Val Lys Gly Asp Asn Leu His
165 170 175
Lys Ile Gly Glu Gly Thr Leu Thr Val Gln Gly Thr Gly Ile Asn Glu
180 185 190
Gly Gly Leu Lys Val Gly Asp Gly Lys Val Val Leu Asn Gln Gln Ala
195 200 205
Asp Asn Lys Gly Gln Val Gln Ala Phe Ser Ser Val Asn Ile Ala Ser
210 215 220
Gly Arg Pro Thr Val Val Leu Thr Asp Glu Arg Gln Val Asn Pro Asp
225 230 235 240
Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr Leu Asp Val Asn Gly Asn
245 250 255
Ser Leu Thr Phe His Gln Leu Lys Ala Ala Asp Tyr Gly Ala Val Leu
260 265 270
Ala Asn Asn Val Asp Lys Arg Ala Thr Ile Thr Leu Asp Tyr Ala Leu
275 280 285
Arg Ala Asp Lys Val Ala Leu Asn Gly Trp Ser Glu Ser Gly Lys Gly
290 295 300
Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn Pro Tyr Thr Asn Thr Thr
305 310 315 320
Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr Gly Tyr Phe Pro Thr Asp
325 330 335
Gln Ser Ser Asn Ala Thr Trp Glu Phe Val Gly His Ser Gln Gly Asp
340 345 350
Ala Gln Lys Leu Val Ala Asp Arg Phe Asn Thr Ala Gly Tyr Leu Phe
355 360 365
His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp Asn Arg Leu Pro Glu
370 375 380
Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala Ala Asp Ile Ser Gly
385 390 395 400
Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu Gln Gly His Pro Val
405 410 415
Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp Lys Leu Ala Ala Ser
420 425 430
Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser Phe Ser Gln Glu Asp
435 440 445
Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu Ser Leu Lys Asn Thr
450 455 460
Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn Thr Thr Ile Gln Ala
465 470 475 480
Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg Val Phe Ile Asp Lys
485 490 495
Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu Glu Gly Thr Ser Val
500 505 510
Ala Thr Lys Asp Ala Asp Lys Ser Val Phe Asn Gly Thr Val Asn Leu
515 520 525
Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile Phe Asn Gly Gly Ile
530 535 540
Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser Asp Ser Ala Val Leu
545 550 555 560
Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn Leu Asn Lys Gly Ala
565 570 575
Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp Gly Pro Val Asn Ile
580 585 590
Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro Asp Glu Val Ser His
595 600 605
Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser Trp Asn Leu Lys Gly
610 615 620
Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser Met Leu Ser Gly Asn
625 630 635 640
Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu Gly Gly Glu Gly Glu
645 650 655
Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met Leu Tyr Ser Leu Phe
660 665 670
Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala
675 680 685
Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr
690 695 700
Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly
705 710 715 720
Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln
725 730 735
Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val
740 745 750
Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe
755 760 765
Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser
770 775 780
Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg Val
785 790 795 800
Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp
805 810 815
Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp
820 825 830
Gly Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn
835 840 845
Phe Ile Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg
850 855 860
Asp Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser
865 870 875 880
Gly Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met
885 890 895
Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly
900 905 910
Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser
915 920 925
Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe
930 935 940
Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu
945 950 955 960
Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg
965 970 975
Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His Leu
980 985 990
Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp Gly Arg
995 1000 1005
Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met Asp Val
1010 1015 1020
Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg Thr Gly
1025 1030 1035
Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser Leu Thr
1040 1045 1050
Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp Ser Ala
1055 1060 1065
Asp Val His Leu Lys Asp Ala Ala Gly Glu His Gln Ile Asn Gly
1070 1075 1080
Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu Asn Ala Arg
1085 1090 1095
Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu Arg Ser Ala
1100 1105 1110
Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn Ile Arg
1115 1120 1125
Tyr SerPhe
1130
<210>5
<211>1131
<212>PRT
<213>大肠杆菌
<400>5
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Cys Gly Ser Gly Ser Gly Asn Asp Ala
50 55 60
Pro Val Thr Phe Arg Thr Ser Glu Gly Gly Ala Leu Glu Trp Ser Phe
65 70 75 80
Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr Gln Gly Thr Thr Thr Tyr
85 90 95
Ala Met His Gly Gln Gln Gly Asn Asp Leu Asn Ala Gly Lys Asn Leu
100 105 110
Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn Leu Lys Asp Ser Val Ser
115 120 125
Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp Asn Tyr Thr Val Thr Thr
130 135 140
Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly Ile Val Val Asp Asn Gly
145 150 155 160
Val Ser Val Asn Trp Gln Val Asn Gly Val Lys Gly Asp Asn Leu His
165 170 175
Lys Ile Gly Glu Gly Thr Leu Thr Val Gln Gly Thr Gly Ile Asn Glu
180 185 190
Gly Gly Leu Lys Val Gly Asp Gly Lys Val Val Leu Asn Gln Gln Ala
195 200 205
Asp Asn Lys Gly Gln Val Gln Ala Phe Ser Ser Val Asn Ile Ala Ser
210 215 220
Gly Arg Pro Thr Val Val Leu Thr Asp Glu Arg Gln Val Asn Pro Asp
225 230 235 240
Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr Leu Asp Val Asn Gly Asn
245 250 255
Ser Leu Thr Phe His Gln Leu Lys Ala Ala Asp Tyr Gly Ala Val Leu
260 265 270
Ala Asn Asn Val Asp Lys Arg Ala Thr Ile Thr Leu Asp Tyr Ala Leu
275 280 285
Arg Ala Asp Lys Val Ala Leu Asn Gly Trp Ser Glu Ser Gly Lys Gly
290 295 300
Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn Pro Tyr Thr Asn Thr Thr
305 310 315 320
Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr Gly Tyr Phe Pro Thr Asp
325 330 335
Gln Ser Ser Asn Ala Thr Trp Glu Phe Val Gly His Ser Gln Gly Asp
340 345 350
Ala Gln Lys Leu Val Ala Asp Arg Phe Asn Thr Ala Gly Tyr Leu Phe
355 360 365
His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp Asn Arg Leu Pro Glu
370 375 380
Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala Ala Asp Ile Ser Gly
385 390 395 400
Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu Gln Gly His Pro Val
405 410 415
Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp Lys Leu Ala Ala Ser
420 425 430
Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser Phe Ser Gln Glu Asp
435 440 445
Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu Ser Leu Lys Asn Thr
450 455 460
Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn Thr Thr Ile Gln Ala
465 470 475 480
Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg Val Phe Ile Asp Lys
485 490 495
Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu Glu Gly Thr Ser Val
500 505 510
Ala Thr Lys Asp Ala Asp Lys Ser Val Phe Asn Gly Thr Val Asn Leu
515 520 525
Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile Phe Asn Gly Gly Ile
530 535 540
Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser Asp Ser Ala Val Leu
545 550 555 560
Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn Leu Asn Lys Gly Ala
565 570 575
Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp Gly Pro Val Asn Ile
580 585 590
Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro Asp Glu Val Ser His
595 600 605
Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser Trp Asn Leu Lys Gly
610 615 620
Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser Met Leu Ser Gly Asn
625 630 635 640
Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu Gly Gly Glu Gly Glu
645 650 655
Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met Leu Tyr Ser Leu Phe
660 665 670
Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala
675 680 685
Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr
690 695 700
Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly
705 710 715 720
Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln
725 730 735
Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val
740 745 750
Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe
755 760 765
Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser
770 775 780
Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg Val
785 790 795 800
Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp
805 810 815
Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp
820 825 830
Gly Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn
835 840 845
Phe Ile Thr Glu Val Gly Ser Leu Asn Lys Arg Met Gly Asp Leu Arg
850 855 860
Asp Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser
865 870 875 880
Gly Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met
885 890 895
Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly
900 905 910
Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser
915 920 925
Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe
930 935 940
Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu
945 950 955 960
Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg
965 970 975
Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His Leu
980 985 990
Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp Gly Arg
995 1000 1005
Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met Asp Val
1010 1015 1020
Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg Thr Gly
1025 1030 1035
Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser Leu Thr
1040 1045 1050
Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp Ser Ala
1055 1060 1065
Asp Val His Leu Lys Asp Ala Ala Gly Glu His Gln Ile Asn Gly
1070 1075 1080
Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu Asn Ala Arg
1085 1090 1095
Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu Arg Ser Ala
1100 1105 1110
Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn Ile Arg
1115 1120 1125
Tyr Ser Phe
1130
<210>6
<211>1314
<212> PRT
<213>大肠杆菌
<400>6
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Gly Ser Gly Ser Ser Ala Gly Ser Gly Ser Gly
530 535 540
Asn Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn
545 550 555 560
Val Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp
565 570 575
Gly Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu
580 585 590
Thr Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val
595 600 605
Ala Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro
610 615 620
Thr Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp
625 630 635 640
Arg Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr
645 650 655
Leu Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp
660 665 670
Ser Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr
675 680 685
Leu Glu Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val
690 695 700
Phe Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn
705 710 715 720
Asp Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile
725 730 735
Ser Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala
740 745 750
Leu Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val
755 760 765
Ser Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser
770 775 780
Arg Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala
785 790 795 800
Gly Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro
805 810 815
Tyr Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val
820 825 830
Thr Leu Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn
835 840 845
Gln Met Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly
850 855 860
Ser Leu Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp
865 870 875 880
Ser Met Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr
885 890 895
Ile Val Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr
900 905 910
Asp Asn Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu
915 920 925
Asn Lys Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp
930 935 940
Asn Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr
945 950 955 960
Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu
965 970 975
Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile
980 985 990
Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly
995 1000 1005
Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr
1010 1015 1020
Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu Val Asn Asn
1025 1030 1035
Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala
1040 1045 1050
Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp Gly
1055 1060 1065
Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala Asp Arg
1070 1075 1080
Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met Ala
1085 1090 1095
Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly Lys
1100 1105 1110
Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe Arg
1115 1120 1125
Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu
1130 1135 1140
Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe
1145 1150 1155
Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr
1160 1165 1170
His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val
1175 1180 1185
Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly
1190 1195 1200
Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly
1205 1210 1215
Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp
1220 1225 1230
Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr
1235 1240 1245
Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His Gln
1250 1255 1260
Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu
1265 1270 1275
Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu
1280 1285 1290
Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala
1295 1300 1305
Asn Ile Arg Tyr Ser Phe
1310
<210> 7
<211>1314
<212>PRT
<213>大肠杆菌
<400>7
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Gly Ser Gly Ser Ser Ala Gly Ser Gly Ser Gly
530 535 540
Asn Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn
545 550 555 560
Val Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp
565 570 575
Gly Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu
580 585 590
Thr Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val
595 600 605
Ala Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro
610 615 620
Thr Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp
625 630 635 640
Arg Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr
645 650 655
Leu Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp
660 665 670
Ser Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr
675 680 685
Leu Glu Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val
690 695 700
Phe Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn
705 710 715 720
Asp Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile
725 730 735
Ser Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala
740 745 750
Leu Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val
755 760 765
Ser Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser
770 775 780
Arg Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala
785 790 795 800
Gly Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro
805 810 815
Tyr Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val
820 825 830
Thr Leu Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn
835 840 845
Gln Met Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly
850 855 860
Ser Leu Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp
865 870 875 880
Ser Met Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr
885 890 895
Ile Val Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr
900 905 910
Asp Asn Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu
915 920 925
Asn Lys Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp
930 935 940
Asn Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr
945 950 955 960
Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu
965 970 975
Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile
980 985 990
Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly
995 1000 1005
Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr
1010 1015 1020
Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu Val Gly Ser
1025 1030 1035
Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala
1040 1045 1050
Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp Gly
1055 1060 1065
Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala Asp Arg
1070 1075 1080
Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met Ala
1085 1090 1095
Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly Lys
1100 1105 1110
Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe Arg
1115 1120 1125
Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu
1130 1135 1140
Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe
1145 1150 1155
Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr
1160 1165 1170
His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val
1175 1180 1185
Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly
1190 1195 1200
Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly
1205 1210 1215
Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp
1220 1225 1230
Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr
1235 1240 1245
Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His Gln
1250 1255 1260
Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu
1265 1270 1275
Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu
1280 1285 1290
Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala
1295 1300 1305
Asn Ile Arg Tyr Ser Phe
1310
<210>8
<211>1350
<212>PRT
<213>大肠杆菌
<400>8
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly Ser Gly Ser Ser Ala Gly Ser Gly Ser Gly Asn Arg Ser
660 665 670
Phe Thr Phe Asp Arg Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly
675 680 685
Arg Asn Ala Thr Leu Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val
690 695 700
Thr Leu Gly Asp Ser Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly
705 710 715 720
Thr Ala Phe Thr Leu Glu Glu Gly Thr Ser Val Ala Thr Lys Asp Ala
725 730 735
Asp Lys Ser Val Phe Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val
740 745 750
Leu Asn Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser
755 760 765
Thr Val Asn Ile Ser Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu
770 775 780
Thr Ser Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser
785 790 795 800
Gln Ser Phe Val Ser Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu
805 810 815
Ser Leu Asn Ser Arg Pro Asp Glu Val Ser His Thr Leu Leu Pro Val
820 825 830
Tyr Asp Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu
835 840 845
Asn Val Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp
850 855 860
Lys Gly Thr Val Thr Leu Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu
865 870 875 880
Thr Leu Gln Asn Gln Met Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn
885 890 895
Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val Ser Met Thr
900 905 910
Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly Asn Met Lys
915 920 925
Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr
930 935 940
Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser Ala Phe Val Met
945 950 955 960
Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val Ile Asn Lys Ser Ala
965 970 975
Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser
980 985 990
Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr
995 1000 1005
Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser
1010 1015 1020
Asp Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys Lys
1025 1030 1035
Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln
1040 1045 1050
Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe
1055 1060 1065
Ile Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg
1070 1075 1080
Asp Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly
1085 1090 1095
Ser Gly Ser Ala Asp Gly Gly Phe Thr Asp His TyrThr Leu Leu
1100 1105 1110
Gln Met Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu
1115 1120 1125
Phe Thr Gly Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala
1130 1135 1140
Asp Leu Tyr Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr
1145 1150 1155
Ala Ser Gly Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala
1160 1165 1170
Lys Tyr Ile His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly
1175 1180 1185
Ala Gly Lys Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala
1190 1195 1200
Glu Val Gly Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu
1205 1210 1215
Pro Gln Ala Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe
1220 1225 1230
Asn Trp Asn Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser
1235 1240 1245
Val Asn Pro Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr
1250 1255 1260
Phe Ser Gly Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His
1265 1270 1275
Tyr Glu Phe Asp Leu Thr Asp Ser Ala Asp Val His Leu Lys Asp
1280 1285 1290
Ala Ala Gly Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met
1295 1300 1305
Leu Tyr Gly Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg
1310 1315 1320
Leu Gly Leu Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr
1325 1330 1335
Asp Asp Ala Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1340 1345 1350
<210>9
<211>1358
<212>PRT
<213>大肠杆菌
<400>9
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
660 665 670
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
675 680 685
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
690 695 700
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
705 710 715 720
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Gly
725 730 735
Ser Gly Ser Ser Ala Gly Ser Gly Ser Gly Ser Val Phe Asn Gly Thr
740 745 750
Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile Phe Asn
755 760 765
Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser Asp Ser
770 775 780
Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn Leu Asn
785 790 795 800
Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp Gly Pro
805 810 815
Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro Asp Glu
820 825 830
Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser Trp Asn
835 840 845
Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser Met Leu
850 855 860
Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu Gly Gly
865 870 875 880
Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met Leu Tyr
885 890 895
Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu Asn Ala
900 905 910
Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met Asn Gly
915 920 925
Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val Gly Phe
930 935 940
Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu Asp
945 950 955 960
Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys Ala Asp
965 970 975
Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp
980 985 990
Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro
995 1000 1005
Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala
1010 1015 1020
Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser
1025 1030 1035
Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr
1040 1045 1050
Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe
1055 1060 1065
Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu Val Asn Asn Leu
1070 1075 1080
Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala Gly
1085 1090 1095
Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp Gly Gly
1100 1105 1110
Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala Asp Arg Lys
1115 1120 1125
His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met Ala Thr
1130 1135 1140
Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly Lys Thr
1145 1150 1155
Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe Arg Ser
1160 1165 1170
Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu Asn
1175 1180 1185
Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg
1190 1195 1200
Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His
1205 1210 1215
Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp
1220 1225 1230
Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met
1235 1240 1245
Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg
1250 1255 1260
Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser
1265 1270 1275
Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp
1280 1285 1290
Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His Gln Ile
1295 1300 1305
Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu Asn
1310 1315 1320
Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu Arg
1325 1330 1335
Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn
1340 1345 1350
Ile Arg Tyr Ser Phe
1355
<210>10
<211>1366
<212>PRT
<213>大肠杆菌
<400>10
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
660 665 670
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
675 680 685
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
690 695 700
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
705 710 715 720
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser
725 730 735
Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu
740 745 750
Glu Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe
755 760 765
Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp
770 775 780
Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser
785 790 795 800
Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu
805 810 815
Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser
820 825 830
Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg
835 840 845
Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly
850 855 860
Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr
865 870 875 880
Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr
885 890 895
Leu Gly Gly Ser Gly Ser Ser Ala Gly Ser Gly Ser Gly Tyr Arg Asn
900 905 910
Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val Ser Met Thr
915 920 925
Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly Asn Met Lys
930 935 940
Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr
945 950 955 960
Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser Ala Phe Val Met
965 970 975
Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val Ile Asn Lys Ser Ala
980 985 990
Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser
995 1000 1005
Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala
1010 1015 1020
Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg Val Val Gly Phe
1025 1030 1035
Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys
1040 1045 1050
Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly
1055 1060 1065
Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn
1070 1075 1080
Phe Ile Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu
1085 1090 1095
Arg Asp Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn
1100 1105 1110
Gly Ser Gly Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu
1115 1120 1125
Leu Gln Met Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp
1130 1135 1140
Leu Phe Thr Gly Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser
1145 1150 1155
Ala Asp Leu Tyr Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe
1160 1165 1170
Tyr Ala Ser Gly Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile
1175 1180 1185
Ala Lys Tyr Ile His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala
1190 1195 1200
Gly Ala Gly Lys Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly
1205 1210 1215
Ala Glu Val Gly Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val
1220 1225 1230
Glu Pro Gln Ala Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr
1235 1240 1245
Phe Asn Trp Asn Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn
1250 1255 1260
Ser Val Asn Pro Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys
1265 1270 1275
Thr Phe Ser Gly Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu
1280 1285 1290
His Tyr Glu Phe Asp Leu Thr Asp Ser Ala Asp Val His Leu Lys
1295 1300 1305
Asp Ala Ala Gly Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg
1310 1315 1320
Met Leu Tyr Gly Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr
1325 1330 1335
Arg Leu Gly Leu Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn
1340 1345 1350
Thr Asp Asp Ala Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1355 1360 1365
<210>11
<211>1383
<212>PRT
<213>大肠杆菌
<400>11
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
660 66 5670
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
675 680 685
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
690 695 700
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
705 710 715 720
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser
725 730 735
Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu
740 745 750
Glu Glu Gly Thr Ser Val Ala Gly Ser Gly Ser Ser Ala Gly Ser Gly
755 760 765
Ser Gly Lys Ser Val Phe Asn Gly Thr Val Asn Leu Asp Asn Gln Ser
770 775 780
Val Leu Asn Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn
785 790 795 800
Ser Thr Val Asn Ile Ser Ser Asp Ser Ala Val Leu Gly Asn Ser Thr
805 810 815
Leu Thr Ser Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala
820 825 830
Ser Gln Ser Phe Val Ser Asp Gly Pro Val Asn Ile Ser Asp Ala Thr
835 840 845
Leu Ser Leu Asn Ser Arg Pro Asp Glu Val Ser His Thr Leu Leu Pro
850 855 860
Val Tyr Asp Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg
865 870 875 880
Leu Asn Val Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile Asn Val Gln
885 890 895
Asp Lys Gly Thr Val Thr Leu Gly Gly Glu Gly Glu Leu Ser Pro Asp
900 905 910
Leu Thr Leu Gln Asn Gln Met Leu Tyr Ser Leu Phe Asn Gly Tyr Arg
915 920 925
Asn Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val Ser Met
930 935 940
Thr Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly Asn Met
945 950 955 960
Lys Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr Ser Pro Phe
965 970 975
Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser Ala Phe Val
980 985 990
Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val Ile Asn Lys Ser
995 1000 1005
Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe Leu Lys Lys
1010 1015 1020
Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser Ala Pro
1025 1030 1035
Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg Val Val
1040 1045 1050
Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp
1055 1060 1065
Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn
1070 1075 1080
Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr
1085 1090 1095
Asn Asn Phe Ile Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly
1100 1105 1110
Asp Leu Arg Asp Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu
1115 1120 1125
Leu Asn Gly Ser Gly Ser Ala Asp Gly Gly Phe Thr Asp His Tyr
1130 1135 1140
Thr Leu Leu Gln Met Gly Ala Asp Arg Lys His Glu Leu Gly Ser
1145 1150 1155
Met Asp Leu Phe Thr Gly Val Met Ala Thr Tyr Thr Asp Thr Asp
1160 1165 1170
Ala Ser Ala Asp Leu Tyr Ser Gly Lys Thr Lys Ser Trp Gly Gly
1175 1180 1185
Gly Phe Tyr Ala Ser Gly Leu Phe Arg Ser Gly Ala Tyr Phe Asp
1190 1195 1200
Val Ile Ala Lys Tyr Ile His Asn Glu Asn Lys Tyr Asp Leu Asn
1205 1210 1215
Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg Ser His Ser Leu Tyr
1220 1225 1230
Ala Gly Ala Glu Val Gly Tyr Arg Tyr His Leu Thr Asp Thr Thr
1235 1240 1245
Phe Val Glu Pro Gln Ala Glu Leu Val Trp Gly Arg Leu Gln Gly
1250 1255 1260
Gln Thr Phe Asn Trp Asn Asp Ser Gly Met Asp Val Ser Met Arg
1265 1270 1275
Arg Asn Ser Val Asn Pro LeuV al Gly Arg Thr Gly Val Val Ser
1280 1285 1290
Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser Leu Thr Ala Arg Ala
1295 1300 1305
Gly Leu His Tyr Glu Phe Asp Leu Thr Asp Ser Ala Asp Val His
1310 1315 1320
Leu Lys Asp Ala Ala Gly Glu His Gln Ile Asn Gly Arg Lys Asp
1325 1330 1335
Ser Arg Met Leu Tyr Gly Val Gly Leu Asn Ala Arg Phe Gly Asp
1340 1345 1350
Asn Thr Arg Leu Gly Leu Glu Val Glu Arg Ser Ala Phe Gly Lys
1355 1360 1365
Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1370 1375 1380
<210>12
<211>539
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>12
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Met Thr Glu Gln Gln Trp Asn Phe Ala
50 55 60
Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser Ile
65 70 75 80
His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala
85 90 95
Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys
100 105 110
Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala
115 120 125
Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly Asn
130 135 140
Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Ala Asp Lys Leu Val
145 150 155 160
Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe
165 170 175
Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser
180 185 190
Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg Val
195 200 205
Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp
210 215 220
Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp
225 230 235 240
Gly Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn
245 250 255
Phe Ile Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg
260 265 270
Asp Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser
275 280 285
Gly Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met
290 295 300
Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly
305 310 315 320
Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser
325 330 335
Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe
340 345 350
Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu
355 360 365
Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg
370 375 380
Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His Leu
385 390 395 400
Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp Gly Arg
405 410 415
Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met Asp Val Ser
420 425 430
Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg Thr Gly Val Val
435 440 445
Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser Leu Thr Ala Arg Ala
450 455 460
Gly Leu His Tyr Glu Phe Asp Leu Thr Asp Ser Ala Asp Val His Leu
465 470 475 480
Lys Asp Ala Ala Gly Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg
485 490 495
Met Leu Tyr Gly Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg
500 505 510
Leu Gly Leu Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp
515 520 525
Asp Ala Ile Asn Ala Asn Ile Arg Tyr Ser Phe
530 535
<210>13
<211>1225
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>13
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Met Thr Glu Gln Gln Trp Asn Phe Ala
50 55 60
Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser Ile
65 70 75 80
His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala
85 90 95
Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys
100 105 110
Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala
115 120 125
Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly Asn
130 135 140
Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Asn Asp Ala Pro Val
145 150 155 160
Thr Phe Arg Thr Ser Glu Gly Gly Ala Leu Glu Trp Ser Phe Asn Ser
165 170 175
Ser Thr Gly Ala Gly Ala Leu Thr Gln Gly Thr Thr Thr Tyr Ala Met
180 185 190
His Gly Gln Gln Gly Asn Asp Leu Asn Ala Gly Lys Asn Leu Ile Phe
195 200 205
Gln Gly Gln Asn Gly Gln Ile Asn Leu Lys Asp Ser Val Ser Gln Gly
210 215 220
Ala Gly Ser Leu Thr Phe Arg Asp Asn Tyr Thr Val Thr Thr Ser Asn
225 230 235 240
Gly Ser Thr Trp Thr Gly Ala Gly Ile Val Val Asp Asn Gly Val Ser
245 250 255
Val Asn Trp Gln Val Asn Gly Val Lys Gly Asp Asn Leu His Lys Ile
260 265 270
Gly Glu Gly Thr Leu Thr Val Gln Gly Thr Gly Ile Asn Glu Gly Gly
275 280 285
Leu Lys Val Gly Asp Gly Lys Val Val Leu Asn Gln Gln Ala Asp Asn
290 295 300
Lys Gly Gln Val Gln Ala Phe Ser Ser Val Asn Ile Ala Ser Gly Arg
305 310 315 320
Pro Thr Val Val Leu Thr Asp Glu Arg Gln Val Asn Pro Asp Thr Val
325 330 335
Ser Trp Gly Tyr Arg Gly Gly Thr Leu Asp Val Asn Gly Asn Ser Leu
340 345 350
Thr Phe His Gln Leu Lys Ala Ala Asp Tyr Gly Ala Val Leu Ala Asn
355 360 365
Asn Val Asp Lys Arg Ala Thr Ile Thr Leu Asp Tyr Ala Leu Arg Ala
370 375 380
Asp Lys Val Ala Leu Asn Gly Trp Ser Glu Ser Gly Lys Gly Thr Ala
385 390 395 400
Gly Asn Leu Tyr Lys Tyr Asn Asn Pro Tyr Thr Asn Thr Thr Asp Tyr
405 410 415
Phe Ile Leu Lys Gln Ser Thr Tyr Gly Tyr Phe Pro Thr Asp Gln Ser
420 425 430
Ser Asn Ala Thr Trp Glu Phe Val Gly His Ser Gln Gly Asp Ala Gln
435 440 445
Lys Leu Val Ala Asp Arg Phe Asn Thr Ala Gly Tyr Leu Phe His Gly
450 455 460
Gln Leu Lys Gly Asn Leu Asn Val Asp Asn Arg Leu Pro Glu Gly Val
465 470 475 480
Thr Gly Ala Leu Val Met Asp Gly Ala Ala Asp Ile Ser Gly Thr Phe
485 490 495
Thr Gln Glu Asn Gly Arg Leu Thr Leu Gln Gly His Pro Val Ile His
500 505 510
Ala Tyr Asn Thr Gln Ser Val Ala Asp Lys Leu Ala Ala Ser Gly Asp
515 520 525
His Ser Val Leu Thr Gln Pro Thr Ser Phe Ser Gln Glu Asp Trp Glu
530 535 540
Asn Arg Ser Phe Thr Phe Asp Arg Leu Ser Leu Lys Asn Thr Asp Phe
545 550 555 560
Gly Leu Gly Arg Asn Ala Thr Leu Asn Thr Thr Ile Gln Ala Asp Asn
565 570 575
Ser Ser Val Thr Leu Gly Asp Ser Arg Val Phe Ile Asp Lys Asn Asp
580 585 590
Gly Gln Gly Thr Ala Phe Thr Leu Glu Glu Gly Thr Ser Val Ala Thr
595 600 605
Lys Asp Ala Asp Lys Ser Val Phe Asn Gly Thr Val Asn Leu Asp Asn
610 615 620
Gln Ser Val Leu Asn Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln Ala
625 630 635 640
Asn Asn Ser Thr Val Asn Ile Ser Ser Asp Ser Ala Val Leu Gly Asn
645 650 655
Ser Thr Leu Thr Ser Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn Ala
660 665 670
Leu Ala Ser Gln Ser Phe Val Ser Asp Gly Pro Val Asn Ile Ser Asp
675 680 685
Ala Thr Leu Ser Leu Asn Ser Arg Pro Asp Glu Val Ser His Thr Leu
690 695 700
Leu Pro Val Tyr Asp Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp Asp
705 710 715 720
Ala Arg Leu Asn Val Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile Asn
725 730 735
Val Gln Asp Lys Gly Thr Val Thr Leu Gly Gly Glu Gly Glu Leu Ser
740 745 750
Pro Asp Leu Thr Leu Gln Asn Gln Met Leu Tyr Ser Leu Phe Asn Gly
755 760 765
Tyr Arg Asn Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val
770 775 780
Ser Met Thr Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly
785 790 795 800
Asn Met Lys Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr Ser
805 810 815
Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser Ala
820 825 830
Phe Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val Ile Asn
835 840 845
Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe Leu Lys
850 855 860
Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser Ala Pro
865 870 875 880
Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg Val Val Gly
885 890 895
Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys
900 905 910
Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln
915 920 925
Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile
930 935 940
Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile
945 950 955 960
Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser
965 970 975
Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala
980 985 990
Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met
995 1000 1005
Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly
1010 1015 1020
Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe
1025 1030 1035
Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn
1040 1045 1050
Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn
1055 1060 1065
Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg
1070 1075 1080
Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu
1085 1090 1095
Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser
1100 1105 1110
Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val
1115 1120 1125
Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp
1130 1135 1140
Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu
1145 1150 1155
Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His
1160 1165 1170
Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly
1175 1180 1185
Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val
1190 1195 1200
Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn
1205 1210 1215
Ala Asn Ile Arg Tyr Ser Phe
1220 1225
<210>14
<211>1225
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>14
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Met Thr Glu Gln Gln Trp Asn Phe Ala
50 55 60
Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser Ile
65 70 75 80
His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala
85 90 95
Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys
100 105 110
Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala
115 120 125
Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly Asn
130 135 140
Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Asn Asp Ala Pro Val
145 150 155 160
Thr Phe Arg Thr Ser Glu Gly Gly Ala Leu Glu Trp Ser Phe Asn Ser
165 170 175
Ser Thr Gly Ala Gly Ala Leu Thr Gln Gly Thr Thr Thr Tyr Ala Met
180 185 190
His Gly Gln Gln Gly Asn Asp Leu Asn Ala Gly Lys Asn Leu Ile Phe
195 200 205
Gln Gly Gln Asn Gly Gln Ile Asn Leu Lys Asp Ser Val Ser Gln Gly
210 215 220
Ala Gly Ser Leu Thr Phe Arg Asp Asn Tyr Thr Val Thr Thr Ser Asn
225 230 235 240
Gly Ser Thr Trp Thr Gly Ala Gly Ile Val Val Asp Asn Gly Val Ser
245 250 255
Val Asn Trp Gln Val Asn Gly Val Lys Gly Asp Asn Leu His Lys Ile
260 265 270
Gly Glu Gly Thr Leu Thr Val Gln Gly Thr Gly Ile Asn Glu Gly Gly
275 280 285
Leu Lys Val Gly Asp Gly Lys Val Val Leu Asn Gln Gln Ala Asp Asn
290 295 300
Lys Gly Gln Val Gln Ala Phe Ser Ser Val Asn Ile Ala Ser Gly Arg
305 310 315 320
Pro Thr Val Val Leu Thr Asp Glu Arg Gln Val Asn Pro Asp Thr Val
325 330 335
Ser Trp Gly Tyr Arg Gly Gly Thr Leu Asp Val Asn Gly Asn Ser Leu
340 345 350
Thr Phe His Gln Leu Lys Ala Ala Asp Tyr Gly Ala Val Leu Ala Asn
355 360 365
Asn Val Asp Lys Arg Ala Thr Ile Thr Leu Asp Tyr Ala Leu Arg Ala
370 375 380
Asp Lys Val Ala Leu Asn Gly Trp Ser Glu Ser Gly Lys Gly Thr Ala
385 390 395 400
Gly Asn Leu Tyr Lys Tyr Asn Asn Pro Tyr Thr Asn Thr Thr Asp Tyr
405 410 415
Phe Ile Leu Lys Gln Ser Thr Tyr Gly Tyr Phe Pro Thr Asp Gln Ser
420 425 430
Ser Asn Ala Thr Trp Glu Phe Val Gly His Ser Gln Gly Asp Ala Gln
435 440 445
Lys Leu Val Ala Asp Arg Phe Asn Thr Ala Gly Tyr Leu Phe His Gly
450 455 460
Gln Leu Lys Gly Asn Leu Asn Val Asp Asn Arg Leu Pro Glu Gly Val
465 470 475 480
Thr Gly Ala Leu Val Met Asp Gly Ala Ala Asp Ile Ser Gly Thr Phe
485 490 495
Thr Gln Glu Asn Gly Arg Leu Thr Leu Gln Gly His Pro Val Ile His
500 505 510
Ala Tyr Asn Thr Gln Ser Val Ala Asp Lys Leu Ala Ala Ser Gly Asp
515 520 525
His Ser Val Leu Thr Gln Pro Thr Ser Phe Ser Gln Glu Asp Trp Glu
530 535 540
Asn Arg Ser Phe Thr Phe Asp Arg Leu Ser Leu Lys Asn Thr Asp Phe
545 550 555 560
Gly Leu Gly Arg Asn Ala Thr Leu Asn Thr Thr Ile Gln Ala Asp Asn
565 570 575
Ser Ser Val Thr Leu Gly Asp Ser Arg Val Phe Ile Asp Lys Asn Asp
580 585 590
Gly Gln Gly Thr Ala Phe Thr Leu Glu Glu Gly Thr Ser Val Ala Thr
595 600 605
Lys Asp Ala Asp Lys Ser Val Phe Asn Gly Thr Val Asn Leu Asp Asn
610 615 620
Gln Ser Val Leu Asn Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln Ala
625 630 635 640
Asn Asn Ser Thr Val Asn Ile Ser Ser Asp Ser Ala Val Leu Gly Asn
645 650 655
Ser Thr Leu Thr Ser Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn Ala
660 665 670
Leu Ala Ser Gln Ser Phe Val Ser Asp Gly Pro Val Asn Ile Ser Asp
675 680 685
Ala Thr Leu Ser Leu Asn Ser Arg Pro Asp Glu Val Ser His Thr Leu
690 695 700
Leu Pro Val Tyr Asp Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp Asp
705 710 715 720
Ala Arg Leu Asn Val Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile Asn
725 730 735
Val Gln Asp Lys Gly Thr Val Thr Leu Gly Gly Glu Gly Glu Leu Ser
740 745 750
Pro Asp Leu Thr Leu Gln Asn Gln Met Leu Tyr Ser Leu Phe Asn Gly
755 760 765
Tyr Arg Asn Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val
770 775 780
Ser Met Thr Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly
785 790 795 800
Asn Met Lys Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr Ser
805 810 815
Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser Ala
820 825 830
Phe Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val Ile Asn
835 840 845
Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe Leu Lys
850 855 860
Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser Ala Pro
865 870 875 880
Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg Val Val Gly
885 890 895
Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys
900 905 910
Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln
915 920 925
Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile
930 935 940
Thr Glu Val Gly Ser Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile
945 950 955 960
Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser
965 970 975
Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala
980 985 990
Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe ThrGly Val Met
995 1000 1005
Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly
1010 1015 1020
Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe
1025 1030 1035
Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn
1040 1045 1050
Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn
1055 1060 1065
Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg
1070 1075 1080
Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu
1085 1090 1095
Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser
1100 1105 1110
Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val
1115 1120 1125
Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp
1130 1135 1140
Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu
1145 1150 1155
Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His
1160 1165 1170
Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly
1175 1180 1185
Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val
1190 1195 1200
Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn
1205 1210 1215
Ala Asn Ile Arg Tyr Ser Phe
1220 1225
<210>15
<211>1408
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>15
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
3 5 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Gly Ser Gly Ser Ser Met Thr Glu Gln Gln Trp
530 535 540
Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val
545 550 555 560
Thr Ser Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys
565 570 575
Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val
580 585 590
Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln
595 600 605
Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr
610 615 620
Glu Gly Asn Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Asn Thr
625 630 635 640
Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp
645 650 655
Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala
660 665 670
Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu
675 680 685
Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp
690 695 700
Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser
705 710 715 720
Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu
725 730 735
Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn
740 745 750
Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg
755 760 765
Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu
770 775 780
Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe Asn
785 790 795 800
Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile
805 810 815
Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser
820 825 830
Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn
835 840 845
Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp
850 855 860
Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro
865 870 875 880
Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser
885 890 895
Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser
900 905 910
Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu
915 920 925
Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met
930 935 940
Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu
945 950 955 960
Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met
965 970 975
Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val
980 985 990
Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn
995 1000 1005
Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn
1010 1015 1020
Lys Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp
1025 1030 1035
Asn Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp
1040 1045 1050
Thr Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp
1055 1060 1065
Asn Leu Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val
1070 1075 1080
Thr Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp
1085 1090 1095
Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys
1100 1105 1110
Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr
1115 1120 1125
Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile
1130 1135 1140
Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly
1145 1150 1155
Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met
1160 1165 1170
Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr
1175 1180 1185
Gly Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu
1190 1195 1200
Tyr Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser
1205 1210 1215
Gly Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr
1220 1225 1230
Ile His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly
1235 1240 1245
Lys Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val
1250 1255 1260
Gly Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln
1265 1270 1275
Ala Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp
1280 1285 1290
Asn Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn
1295 1300 1305
Pro Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser
1310 1315 1320
Gly Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu
1325 1330 1335
Phe Asp Leu Thr Asp Ser AlaAsp Val His Leu Lys Asp Ala Ala
1340 1345 1350
Gly Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr
1355 1360 1365
Gly Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly
1370 1375 1380
Leu Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp
1385 1390 1395
Ala Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1400 1405
<210>16
<211>1408
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>16
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Gly Ser Gly Ser Ser Met Thr Glu Gln Gln Trp
530 535 540
Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val
545 550 555 560
Thr Ser Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys
565 570 575
Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val
580 585 590
Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln
595 600 605
Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr
610 615 620
Glu Gly Asn Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Asn Thr
625 630 635 640
Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp
645 650 655
Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala
660 665 670
Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu
675 680 685
Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp
690 695 700
Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser
705 710 715 720
Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu
725 730 735
Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn
740 745 750
Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg
755 760 765
Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu
770 775 780
Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe Asn
785 790 795 800
Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile
805 810 815
Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser
820 825 830
Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn
835 840 845
Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp
850 855 860
Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro
865 870 875 880
Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser
885 890 895
Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser
900 905 910
Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu
915 920 925
Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met
930 935 940
Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu
945 950 955 960
Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met
965 970 975
Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val
980 985 990
Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu ThrThr Asp Asn
995 1000 1005
Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn
1010 1015 1020
Lys Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp
1025 1030 1035
Asn Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp
1040 1045 1050
Thr Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp
1055 1060 1065
Asn Leu Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val
1070 1075 1080
Thr Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp
1085 1090 1095
Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys
1100 1105 1110
Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr
1115 1120 1125
Glu Val Gly Ser Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile
1130 1135 1140
Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly
1145 1150 1155
Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met
1160 1165 1170
Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr
1175 1180 1185
Gly Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu
1190 1195 1200
Tyr Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser
1205 1210 1215
Gly Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr
1220 1225 1230
Ile His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly
1235 1240 1245
Lys Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val
1250 1255 1260
Gly Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln
1265 1270 1275
Ala Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp
1280 1285 1290
Asn Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn
1295 1300 1305
Pro Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser
1310 1315 1320
Gly Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu
1325 1330 1335
Phe Asp Leu Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala
1340 1345 1350
Gly Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr
1355 1360 1365
Gly Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly
1370 1375 1380
Leu Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp
1385 1390 1395
Ala Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1400 1405
<210>17
<211>1444
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>17
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly Ser Gly Ser Ser Met Thr Glu Gln Gln Trp Asn Phe Ala
660 665 670
Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser Ile
675 680 685
His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala
690 695 700
Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys
705 710 715 720
Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala
725 730 735
Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly Asn
740 745 750
Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Asn Arg Ser Phe Thr
755 760 765
Phe Asp Arg Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn
770 775 780
Ala Thr Leu Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu
785 790 795 800
Gly Asp Ser Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala
805 810 815
Phe Thr Leu Glu Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys
820 825 830
Ser Val Phe Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn
835 840 845
Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val
850 855 860
Asn Ile Ser Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser
865 870 875 880
Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser
885 890 895
Phe Val Ser Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu
900 905 910
Asn Ser Arg Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp
915 920 925
Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val
930 935 940
Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly
945 950 955 960
Thr Val Thr Leu Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu
965 970 975
Gln Asn Gln Met Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp
980 985 990
Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr
995 1000 1005
Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu
1010 1015 1020
Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr
1025 1030 1035
Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser Ala Phe Val
1040 1045 1050
Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val Ile Asn Lys
1055 1060 1065
Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe Leu Lys
1070 1075 1080
Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser Ala
1085 1090 1095
Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg Val
1100 1105 1110
Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys Glu
1115 1120 1125
Asp Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg
1130 1135 1140
Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser
1145 1150 1155
Tyr Asn Asn Phe Ile Thr Glu Val Asn Asn Leu Asn Lys Arg Met
1160 1165 1170
Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala Gly Thr Trp Val Arg
1175 1180 1185
Leu Leu Asn Gly Ser Gly Ser Ala Asp Gly Gly Phe Thr Asp His
1190 1195 1200
Tyr Thr Leu Leu Gln Met Gly Ala Asp Arg Lys His Glu Leu Gly
1205 1210 1215
Ser Met Asp Leu Phe Thr Gly Val Met Ala Thr Tyr Thr Asp Thr
1220 1225 1230
Asp Ala Ser Ala Asp Leu Tyr Ser Gly Lys Thr Lys Ser Trp Gly
1235 1240 1245
Gly Gly Phe Tyr Ala Ser Gly Leu Phe Arg Ser Gly Ala Tyr Phe
1250 1255 1260
Asp Val Ile Ala Lys Tyr Ile His Asn Glu Asn Lys Tyr Asp Leu
1265 1270 1275
Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg Ser His Ser Leu
1280 1285 1290
Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His Leu Thr Asp Thr
1295 1300 1305
Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp Gly Arg Leu Gln
1310 1315 1320
Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met Asp Val Ser Met
1325 1330 1335
Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg Thr Gly Val Val
1340 1345 1350
Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser Leu Thr Ala Arg
1355 1360 1365
Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp Ser Ala Asp Val
1370 1375 1380
His Leu Lys Asp Ala Ala Gly Glu His Gln Ile Asn Gly Arg Lys
1385 1390 1395
Asp Ser Arg Met Leu Tyr Gly Val Gly Leu Asn Ala Arg Phe Gly
1400 1405 1410
Asp Asn Thr Arg Leu Gly Leu Glu Val Glu Arg Ser Ala Phe Gly
1415 1420 1425
Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn Ile Arg Tyr Ser
1430 1435 1440
Phe
<210>18
<211>1452
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>18
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
660 665 670
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
675 680 685
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
690 695 700
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
705 710 715 720
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Gly
725 730 735
Ser Gly Ser Ser Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu
740 745 750
Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu
755 760 765
Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly
770 775 780
Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala
785 790 795 800
Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile
805 810 815
Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly
820 825 830
Met Phe Ala Gly Ser Gly Ser Gly Ser Val Phe Asn Gly Thr Val Asn
835 840 845
Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile Phe Asn Gly Gly
850 855 860
Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser Asp Ser Ala Val
865 870 875 880
Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn Leu Asn Lys Gly
885 890 895
Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp Gly Pro Val Asn
900 905 910
Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro Asp Glu Val Ser
915 920 925
His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser Trp Asn Leu Lys
930 935 940
Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser Met Leu Ser Gly
945 950 955 960
Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu Gly Gly Glu Gly
965 970 975
Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met Leu Tyr Ser Leu
980 985 990
Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp
995 1000 1005
Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met Asn Gly Asn
1010 1015 1020
Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val Gly Phe
1025 1030 1035
Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu
1040 1045 1050
Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys
1055 1060 1065
Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn
1070 1075 1080
Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr
1085 1090 1095
Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn
1100 1105 1110
Leu Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr
1115 1120 1125
Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val
1130 1135 1140
Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala
1145 1150 1155
Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu
1160 1165 1170
Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn
1175 1180 1185
Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser
1190 1195 1200
Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly
1205 1210 1215
Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly
1220 1225 1230
Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr
1235 1240 1245
Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly
1250 1255 1260
Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile
1265 1270 1275
His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys
1280 1285 1290
Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly
1295 1300 1305
Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala
1310 1315 1320
Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn
1325 1330 1335
Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro
1340 1345 1350
Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly
1355 1360 1365
Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe
1370 1375 1380
Asp Leu Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly
1385 1390 1395
Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly
1400 1405 1410
Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu
1415 1420 1425
Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala
1430 1435 1440
Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1445 1450
<210>19
<211>1460
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>19
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
660 665 670
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
6 75 680 685
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
690 695 700
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
705 710 715 720
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser
725 730 735
Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu
740 745 750
Glu Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe
755 760 765
Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp
770 775 780
Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser
785 790 795 800
Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu
805 810 815
Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser
820 825 830
Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg
835 840 845
Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly
850 855 860
Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr
865 870 875 880
Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr
885 890 895
Leu Gly Gly Ser Gly Ser Ser Met Thr Glu Gln Gln Trp Asn Phe Ala
900 905 910
Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser Ile
915 920 925
His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala
930 935 940
Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys
945 950 955 960
Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala
965 970 975
Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly Asn
980 985 990
Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Tyr Arg Asn Ile Trp
995 1000 1005
Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp
1010 1015 1020
Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly Asn Met Lys
1025 1030 1035
Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr Ser Pro Phe
1040 1045 1050
Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser Ala Phe
1055 1060 1065
Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val Ile Asn
1070 1075 1080
Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe Leu
1085 1090 1095
Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser
1100 1105 1110
Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg
1115 1120 1125
Val Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys
1130 1135 1140
Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala
1145 1150 1155
Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile
1160 1165 1170
Ser Tyr Asn Asn Phe Ile Thr Glu Val Asn Asn Leu Asn Lys Arg
1175 1180 1185
Met Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala Gly Thr Trp Val
1190 1195 1200
Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp Gly Gly Phe Thr Asp
1205 1210 1215
His Tyr Thr Leu Leu Gln Met Gly Ala Asp Arg Lys His Glu Leu
1220 1225 1230
Gly Ser Met Asp Leu Phe Thr Gly Val Met Ala Thr Tyr Thr Asp
1235 1240 1245
Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly Lys Thr Lys Ser Trp
1250 1255 1260
Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe Arg Ser Gly Ala Tyr
1265 1270 1275
Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu Asn Lys Tyr Asp
1280 1285 1290
Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg Ser His Ser
1295 1300 1305
Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His Leu Thr Asp
1310 1315 1320
Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp Gly Arg Leu
1325 1330 1335
Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met Asp Val Ser
1340 1345 1350
Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg Thr Gly Val
1355 1360 1365
Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser Leu Thr Ala
1370 1375 1380
Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp Ser Ala Asp
1385 1390 1395
Val His Leu Lys Asp Ala Ala Gly Glu His Gln Ile Asn Gly Arg
1400 1405 1410
Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu Asn Ala Arg Phe
1415 1420 1425
Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu Arg Ser Ala Phe
1430 1435 1440
Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn Ile Arg Tyr
1445 1450 1455
Ser Phe
1460
<210>20
<211>1477
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>20
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
660 665 670
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
675 680 685
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
6 90 695 700
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
705 710 715 720
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser
725 730 735
Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu
740 745 750
Glu Glu Gly Thr Ser Val Ala Gly Ser Gly Ser Ser Met Thr Glu Gln
755 760 765
Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly
770 775 780
Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu
785 790 795 800
Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln
805 810 815
Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala
820 825 830
Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala
835 840 845
Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly
850 855 860
Lys Ser Val Phe Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu
865 870 875 880
Asn Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr
885 890 895
Val Asn Ile Ser Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr
900 905 910
Ser Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln
9 15 920 925
Ser Phe Val Ser Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser
930 935 940
Leu Asn Ser Arg Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr
945 950 955 960
Asp Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn
965 970 975
Val Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys
980 985 990
Gly Thr Val Thr Leu Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr
995 1000 1005
Leu Gln Asn Gln Met Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn
1010 1015 1020
Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val Ser Met
1025 1030 1035
Thr Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly Asn
1040 1045 1050
Met Lys Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr Ser
1055 1060 1065
Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser
1070 1075 1080
Ala Phe Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val
1085 1090 1095
Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn
1100 1105 1110
Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu
1115 1120 1125
Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser
1130 1135 1140
Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val
1145 1150 1155
Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln
1160 1165 1170
Val Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe Met
1175 1180 1185
His Ile Ser Tyr Asn Asn Phe Ile Thr Glu Val Asn Asn Leu Asn
1190 1195 1200
Lys Arg Met Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala Gly Thr
1205 1210 1215
Trp Val Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp Gly Gly Phe
1220 1225 1230
Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala Asp Arg Lys His
1235 1240 1245
Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met Ala Thr Tyr
1250 1255 1260
Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly Lys Thr Lys
1265 1270 1275
Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe Arg Ser Gly
1280 1285 1290
Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu Asn Lys
1295 1300 1305
Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg Ser
1310 1315 1320
His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His Leu
1325 1330 1335
Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp Gly
1340 1345 1350
Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met Asp
1355 1360 1365
Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg Thr
1370 1375 1380
Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser Leu
1385 1390 1395
Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp Ser
1400 1405 1410
Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His Gln Ile Asn
1415 1420 1425
Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu Asn Ala
1430 1435 1440
Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu Arg Ser
1445 1450 1455
Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn Ile
1460 1465 1470
Arg Tyr Ser Phe
1475
<210>21
<211>1482
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>21
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
660 665 670
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
675 680 685
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
690 695 700
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
705 710 715 720
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser
725 730 735
Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu
740 745 750
Glu Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe
755 760 765
Asn Gly Gly Ser Gly Ser Ser Met Thr Glu Gln Gln Trp Asn Phe Ala
770 775 780
Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser Ile
785 790 795 800
His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala Ala
805 810 815
Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln Lys
820 825 830
Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu Ala
835 840 845
Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly Asn
850 855 860
Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Thr Val Asn Leu Asp
865 870 875 880
Asn Gln Ser Val Leu Asn Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln
885 890 895
Ala Asn Asn Ser Thr Val Asn Ile Ser Ser Asp Ser Ala Val Leu Gly
900 905 910
Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn
915 920 925
Ala Leu Ala Ser Gln Ser Phe Val Ser Asp Gly Pro Val Asn Ile Ser
930 935 940
Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro Asp Glu Val Ser His Thr
945 950 955 960
Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp
965 970 975
Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile
980 985 990
Asn Val Gln Asp Lys Gly Thr Val Thr Leu Gly Gly Glu Gly Glu Leu
995 1000 1005
Ser Pro Asp Leu Thr Leu Gln Asn Gln Met Leu Tyr Ser Leu Phe
1010 1015 1020
Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp
1025 1030 1035
Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met Asn Gly Asn
1040 1045 1050
Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val Gly Phe
1055 1060 1065
Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu
1070 1075 1080
Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys
1085 1090 1095
Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn
1100 1105 1110
Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr
1115 1120 1125
Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn
1130 1135 1140
Leu Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr
1145 1150 1155
Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val
1160 1165 1170
Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala
1175 1180 1185
Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu
1190 1195 1200
Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn
1205 1210 1215
Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser
1220 1225 1230
Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly
1235 1240 1245
Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly
1250 1255 1260
Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr
1265 1270 1275
Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly
1280 1285 1290
Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile
1295 1300 1305
His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys
1310 1315 1320
Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly
1325 1330 1335
Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala
1340 1345 1350
Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn
1355 1360 1365
Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro
1370 1375 1380
Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly
1385 1390 1395
Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe
1400 1405 1410
Asp Leu Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly
1415 1420 1425
Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly
1430 1435 1440
Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu
1445 1450 1455
Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala
1460 1465 1470
Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1475 1480
<210>22
<211>1424
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>22
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly Ser Gly Ser Ser Val Ile Ala Gly Val Asp Gln Ala Leu
660 665 670
Ala Ala Thr Gly Gln Ala Ser Gln Arg Ala Ala Gly Ala Ser Gly Gly
675 680 685
Val Thr Val Gly Val Gly Val Gly Thr Glu Gln Arg Asn Leu Ser Val
690 695 700
Val Ala Pro Ser Gln Phe Thr Phe Ser Ser Arg Ser Pro Asp Phe Val
705 710 715 720
Asp Glu Thr Ala Gly Gln Ser Trp Cys Ala Ile Leu Gly Leu Asn Gln
725 730 735
Phe His Gly Ser Gly Ser Gly Asn Arg Ser Phe Thr Phe Asp Arg Leu
740 745 750
Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn
755 760 765
Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg
770 775 780
Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu
785 790 795 800
Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe Asn
805 810 815
Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile
820 825 830
Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser
835 840 845
Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn
850 855 860
Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp
865 870 875 880
Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro
885 890 895
Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser
900 905 910
Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser
915 920 925
Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu
930 935 940
Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met
945 950 955 960
Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu
965 970 975
Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met
980 985 990
Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val
995 1000 1005
Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp
1010 1015 1020
Asn Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu
1025 1030 1035
Asn Lys Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His
1040 1045 1050
Asp Asn Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys
1055 1060 1065
Asp Thr Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala
1070 1075 1080
Asp Asn Leu Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp
1085 1090 1095
Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu
1100 1105 1110
Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly
1115 1120 1125
Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile
1130 1135 1140
Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp
1145 1150 1155
Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser
1160 1165 1170
Gly Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln
1175 1180 1185
Met Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe
1190 1195 1200
Thr Gly Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp
1205 1210 1215
Leu Tyr Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala
1220 1225 1230
Ser Gly Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys
1235 1240 1245
Tyr Ile His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala
1250 1255 1260
Gly Lys Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu
1265 1270 1275
Val Gly Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro
1280 1285 1290
Gln Ala Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn
1295 1300 1305
Trp Asn Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser Val
1310 1315 1320
Asn Pro Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe
1325 1330 1335
Ser Gly Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr
1340 1345 1350
Glu Phe Asp Leu Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala
1355 1360 1365
Ala Gly Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu
1370 1375 1380
Tyr Gly Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu
1385 1390 1395
Gly Leu Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp
1400 1405 1410
Asp Ala Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1415 1420
<210>23
<211>1432
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>23
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
660 665 670
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
675 680 685
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
690 695 700
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
705 710 715 720
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Gly
725 730 735
Ser Gly Ser Ser Val Ile Ala Gly Val Asp Gln Ala Leu Ala Ala Thr
740 745 750
Gly Gln Ala Ser Gln Arg Ala Ala Gly Ala Ser Gly Gly Val Thr Val
755 760 765
Gly Val Gly Val Gly Thr Glu Gln Arg Asn Leu Ser Val Val Ala Pro
770 775 780
Ser Gln Phe Thr Phe Ser Ser Arg Ser Pro Asp Phe Val Asp Glu Thr
785 790 795 800
Ala Gly Gln Ser Trp Cys Ala Ile Leu Gly Leu Asn Gln Phe His Gly
805 810 815
Ser Gly Ser Gly Ser Val Phe Asn Gly Thr Val Asn Leu Asp Asn Gln
820 825 830
Ser Val Leu Asn Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln Ala Asn
835 840 845
Asn Ser Thr Val Asn Ile Ser Ser Asp Ser Ala Val Leu Gly Asn Ser
850 855 860
Thr Leu Thr Ser Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn Ala Leu
865 870 875 880
Ala Ser Gln Ser Phe Val Ser Asp Gly Pro Val Asn Ile Ser Asp Ala
885 890 895
Thr Leu Ser Leu Asn Ser Arg Pro Asp Glu Val Ser His Thr Leu Leu
900 905 910
Pro Val Tyr Asp Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp Asp Ala
915 920 925
Arg Leu Asn Val Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile Asn Val
930 935 940
Gln Asp Lys Gly Thr Val Thr Leu Gly Gly Glu Gly Glu Leu Ser Pro
945 950 955 960
Asp Leu Thr Leu Gln Asn Gln Met Leu Tyr Ser Leu Phe Asn Gly Tyr
965 970 975
Arg Asn Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val Ser
980 985 990
Met Thr Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly Asn
995 1000 1005
Met Lys Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr Ser
1010 1015 1020
Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser
1025 1030 1035
Ala Phe Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val
1040 1045 1050
Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn
1055 1060 1065
Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu
1070 1075 1080
Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser
1085 1090 1095
Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val
1100 1105 1110
Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln
1115 1120 1125
Val Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe Met
1130 1135 1140
His Ile Ser Tyr Asn Asn Phe Ile Thr Glu Val Asn Asn Leu Asn
1145 1150 1155
Lys Arg Met Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala Gly Thr
1160 1165 1170
Trp Val Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp Gly Gly Phe
1175 1180 1185
Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala Asp Arg Lys His
1190 1195 1200
Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met Ala Thr Tyr
1205 1210 1215
Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly Lys Thr Lys
1220 1225 1230
Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe Arg Ser Gly
1235 1240 1245
Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu Asn Lys
1250 1255 1260
Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg Ser
1265 1270 1275
His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His Leu
1280 1285 1290
Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp Gly
1295 1300 1305
Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met Asp
1310 1315 1320
Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg Thr
1325 1330 1335
Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser Leu
1340 1345 1350
Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp Ser
1355 1360 1365
Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His Gln Ile Asn
1370 1375 1380
Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu Asn Ala
1385 1390 1395
Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu Arg Ser
1400 1405 1410
Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn Ile
1415 1420 1425
Arg Tyr Ser Phe
1430
<210>24
<211>1440
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>24
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp
530 535 540
Ser Glu Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn
545 550 555 560
Pro Tyr Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr
565 570 575
Gly Tyr Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val
580 585 590
Gly His Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn
595 600 605
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
610 615 620
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
625 630 635 640
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
645 650 655
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
660 665 670
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
675 680 685
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
690 695 700
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
705 710 715 720
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser
725 730 735
Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu
740 745 750
Glu Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe
755 760 765
Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp
770 775 780
Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser
785 790 795 800
Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu
805 810 815
Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser
820 825 830
Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg
835 840 845
Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly
850 855 860
Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr
865 870 875 880
Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr
885 890 895
Leu Gly Gly Ser Gly Ser Ser Val Ile Ala Gly Val Asp Gln Ala Leu
900 905 910
Ala Ala Thr Gly Gln Ala Ser Gln Arg Ala Ala Gly Ala Ser Gly Gly
915 920 925
Val Thr Val Gly Val Gly Val Gly Thr Glu Gln Arg Asn Leu Ser Val
930 935 940
Val Ala Pro Ser Gln Phe Thr Phe Ser Ser Arg Ser Pro Asp Phe Val
945 950 955 960
Asp Glu Thr Ala Gly Gln Ser Trp Cys Ala Ile Leu Gly Leu Asn Gln
965 970 975
Phe His Gly Ser Gly Ser Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu
980 985 990
Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met
995 1000 1005
Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile
1010 1015 1020
Val Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr
1025 1030 1035
Asp Asn Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp
1040 1045 1050
Leu Asn Lys Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly
1055 1060 1065
His Asp Asn Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn
1070 1075 1080
Lys Asp Thr Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr
1085 1090 1095
Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser
1100 1105 1110
Asp Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys Lys
1115 1120 1125
Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln
1130 1135 1140
Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe
1145 1150 1155
Ile Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg
1160 1165 1170
Asp Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly
1175 1180 1185
Ser Gly Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu
1190 1195 1200
Gln Met Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu
1205 1210 1215
Phe Thr Gly Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala
1220 1225 1230
Asp Leu Tyr Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr
1235 1240 1245
Ala Ser Gly Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala
1250 1255 1260
Lys Tyr Ile His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly
1265 1270 1275
Ala Gly Lys Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala
1280 1285 1290
Glu Val Gly Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu
1295 1300 1305
Pro Gln Ala Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe
1310 1315 1320
Asn Trp Asn Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser
1325 1330 1335
Val Asn Pro Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr
1340 1345 1350
Phe Ser Gly Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His
1355 1360 1365
Tyr Glu Phe Asp Leu Thr Asp Ser Ala Asp Val His Leu Lys Asp
1370 1375 1380
Ala Ala Gly Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met
1385 1390 1395
Leu Tyr Gly Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg
1400 1405 1410
Leu Gly Leu Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr
1415 1420 1425
Asp Asp Ala Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1430 1435 1440
<210>25
<211>1226
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>25
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Met Ser Gln Ile Met Tyr Asn Tyr Pro
50 55 60
Ala Met Leu Gly His Ala Gly Asp Met Ala Gly Tyr Ala Gly Thr Leu
65 70 75 80
Gln Ser Leu Gly Ala Glu Ile Ala Val Glu Gln Ala Ala Leu Gln Ser
85 90 95
Ala Trp Gln Gly Asp Thr Gly Ile Thr Tyr Gln Ala Trp Gln Ala Gln
100 105 110
Trp Asn Gln Ala Met Glu Asp Leu Val Arg Ala Tyr His Ala Met Ser
115 120 125
Ser Thr His Glu Ala Asn Thr Met Ala Met Met Ala Arg Asp Thr Ala
130 135 140
Glu Ala Ala Lys Trp Gly Gly Gly Ser Gly Ser Gly Asn Asp Ala Pro
145 150 155 160
Val Thr Phe Arg Thr Ser Glu Gly Gly Ala Leu Glu Trp Ser Phe Asn
165 170 175
Ser Ser Thr Gly Ala Gly Ala Leu Thr Gln Gly Thr Thr Thr Tyr Ala
180 185 190
Met His Gly Gln Gln Gly Asn Asp Leu Asn Ala Gly Lys Asn Leu Ile
195 200 205
Phe Gln Gly Gln Asn Gly Gln Ile Asn Leu Lys Asp Ser Val Ser Gln
210 215 220
Gly Ala Gly Ser Leu Thr Phe Arg Asp Asn Tyr Thr Val Thr Thr Ser
225 230 235 240
Asn Gly Ser Thr Trp Thr Gly Ala Gly Ile Val Val Asp Asn Gly Val
245 250 255
Ser Val Asn Trp Gln Val Asn Gly Val Lys Gly Asp Asn Leu His Lys
260 265 270
Ile Gly Glu Gly Thr Leu Thr Val Gln Gly Thr Gly Ile Asn Glu Gly
275 280 285
Gly Leu Lys Val Gly Asp Gly Lys Val Val Leu Asn Gln Gln Ala Asp
290 295 300
Asn Lys Gly Gln Val Gln Ala Phe Ser Ser Val Asn Ile Ala Ser Gly
305 310 315 320
Arg Pro Thr Val Val Leu Thr Asp Glu Arg Gln Val Asn Pro Asp Thr
325 330 335
Val Ser Trp Gly Tyr Arg Gly Gly Thr Leu Asp Val Asn Gly Asn Ser
340 345 350
Leu Thr Phe His Gln Leu Lys Ala Ala Asp Tyr Gly Ala Val Leu Ala
355 360 365
Asn Asn Val Asp Lys Arg Ala Thr Ile Thr Leu Asp Tyr Ala Leu Arg
370 375 380
Ala Asp Lys Val Ala Leu Asn Gly Trp Ser Glu Ser Gly Lys Gly Thr
385 390 395 400
Ala Gly Asn Leu Tyr Lys Tyr Asn Asn Pro Tyr Thr Asn Thr Thr Asp
405 410 415
Tyr Phe Ile Leu Lys Gln Ser Thr Tyr Gly Tyr Phe Pro Thr Asp Gln
420 425 430
Ser Ser Asn Ala Thr Trp Glu Phe Val Gly His Ser Gln Gly Asp Ala
435 440 445
Gln Lys Leu Val Ala Asp Arg Phe Asn Thr Ala Gly Tyr Leu Phe His
450 455 460
Gly Gln Leu Lys Gly Asn Leu Asn Val Asp Asn Arg Leu Pro Glu Gly
465 470 475 480
Val Thr Gly Ala Leu Val Met Asp Gly Ala Ala Asp Ile Ser Gly Thr
485 490 495
Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu Gln Gly His Pro Val Ile
500 505 510
His Ala Tyr Asn Thr Gln Ser Val Ala Asp Lys Leu Ala Ala Ser Gly
515 520 525
Asp His Ser Val Leu Thr Gln Pro Thr Ser Phe Ser Gln Glu Asp Trp
530 535 540
Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu Ser Leu Lys Asn Thr Asp
545 550 555 560
Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn Thr Thr Ile Gln Ala Asp
565 570 575
Asn Ser Ser Val Thr Leu Gly Asp Ser Arg Val Phe Ile Asp Lys Asn
580 585 590
Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu Glu Gly Thr Ser Val Ala
595 600 605
Thr Lys Asp Ala Asp Lys Ser Val Phe Asn Gly Thr Val Asn Leu Asp
610 615 620
Asn Gln Ser Val Leu Asn Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln
625 630 635 640
Ala Asn Asn Ser Thr Val Asn Ile Ser Ser Asp Ser Ala Val Leu Gly
645 650 655
Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn
660 665 670
Ala Leu Ala Ser Gln Ser Phe Val Ser Asp Gly Pro Val Asn Ile Ser
675 680 685
Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro Asp Glu Val Ser His Thr
690 695 700
Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp
705 710 715 720
Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile
725 730 735
Asn Val Gln Asp Lys Gly Thr Val Thr Leu Gly Gly Glu Gly Glu Leu
740 745 750
Ser Pro Asp Leu Thr Leu Gln Asn Gln Met Leu Tyr Ser Leu Phe Asn
755 760 765
Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr
770 775 780
Val Ser Met Thr Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala
785 790 795 800
Gly Asn Met Lys Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr
805 810 815
Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser
820 825 830
Ala Phe Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val Ile
835 840 845
Asn Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe Leu
850 855 860
Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser Ala
865 870 875 880
Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg Val Val
885 890 895
Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp Gly
900 905 910
Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly
915 920 925
Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe
930 935 940
Ile Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp
945 950 955 960
Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly
965 970 975
Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly
980 985 990
Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val
995 1000 1005
Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser
1010 1015 1020
Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu
1025 1030 1035
Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His
1040 1045 1050
Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln
1055 1060 1065
Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr
1070 1075 1080
Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu
1085 1090 1095
Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp
1100 1105 1110
Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu
1115 1120 1125
Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys
1130 1135 1140
Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp
1145 1150 1155
Leu Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu
1160 1165 1170
His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val
1175 1180 1185
Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu
1190 1195 1200
Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile
1205 1210 1215
Asn Ala Asn Ile Arg Tyr Ser Phe
1220 1225
<210>26
<211>1409
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>26
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Thr Val Asn Asn Glu Leu Gly Tyr Gln Leu Phe
50 55 60
Arg Asp Phe Ala Glu Asn Lys Gly Met Phe Arg Pro Gly Ala Thr Asn
65 70 75 80
Ile Ala Ile Tyr Asn Lys Gln Gly Glu Phe Val Gly Thr Leu Asp Lys
85 90 95
Ala Ala Met Pro Asp Phe Ser Ala Val Asp Ser Glu Ile Gly Val Ala
100 105 110
Thr Leu Ile Asn Pro Gln Tyr Ile Ala Ser Val Lys His Asn Gly Gly
115 120 125
Tyr Thr Asn Val Ser Phe Gly Asp Gly Glu Asn Arg Tyr Asn Ile Val
130 135 140
Asp Arg Asn Asn Ala Pro Ser Leu Asp Phe His Ala Pro Arg Leu Asp
145 150 155 160
Lys Leu Val Thr Glu Val Ala Pro Thr Ala Val Thr Ala Gln Gly Ala
165 170 175
Val Ala Gly Ala Tyr Leu Asp Lys Glu Arg Tyr Pro Val Phe Tyr Arg
180 185 190
Leu Gly Ser Gly Thr Gln Tyr Ile Lys Asp Ser Asn Gly Gln Leu Thr
195 200 205
Lys Met Gly Gly Ala Tyr Ser Trp Leu Thr Gly Gly Thr Val Gly Ser
210 215 220
Leu Ser Ser Tyr Gln Asn Gly Glu Met Ile Ser Thr Ser Ser Gly Leu
225 230 235 240
Val Phe Asp Tyr Lys Leu Asn Gly Ala Met Pro Ile Tyr Gly Glu Ala
245 250 255
Gly Asp Ser Gly Ser Pro Leu Phe Ala Phe Asp Thr Val Gln Asn Lys
260 265 270
Trp Val Leu Val Gly Val Leu Thr Ala Gly Asn Gly Ala Gly Gly Arg
275 280 285
Gly Asn Asn Trp Ala Val Ile Pro Leu Asp Phe Ile Gly Gln Lys Phe
290 295 300
Asn Glu Asp Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly
305 310 315 320
Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr
325 330 335
Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu
340 345 350
Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn
355 360 365
Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp
370 375 380
Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly
385 390 395 400
Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val
405 410 415
Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln
420 425 430
Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val
435 440 445
Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser
450 455 460
Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu
465 470 475 480
Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr
485 490 495
Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala
500 505 510
Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile
515 520 525
Thr Leu Asp Tyr Ala Gly Ser Gly Ser Ser Met Ser Gln Ile Met Tyr
530 535 540
Asn Tyr Pro Ala Met Met Ala His Ala Gly Asp Met Ala Gly Tyr Ala
545 550 555 560
Gly Thr Leu Gln Ser Leu Gly Ala Asp Ile Ala Ser Glu Gln Ala Val
565 570 575
Leu Ser Ser Ala Trp Gln Gly Asp Thr Gly Ile Thr Tyr Gln Gly Trp
580 585 590
Gln Thr Gln Trp Asn Gln Ala Leu Glu Asp Leu Val Arg Ala Tyr Gln
595 600 605
Ser Met Ser Gly Thr His Glu Ser Asn Thr Met Ala Met Leu Ala Arg
610 615 620
Asp Gly Ala Glu Ala Ala Lys Trp Gly Gly Gly Ser Gly Ser Gly Asn
625 630 635 640
Thr Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val
645 650 655
Asp Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly
660 665 670
Ala Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr
675 680 685
Leu Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala
690 695 700
Asp Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr
705 710 715 720
Ser Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg
725 730 735
Leu Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu
740 745 750
Asn Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser
755 760 765
Arg Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu
770 775 780
Glu Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe
785 790 795 800
Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp
805 810 815
Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser
820 825 830
Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu
835 840 845
Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser
850 855 860
Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg
865 870 875 880
Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly
885 890 895
Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr
900 905 910
Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr
915 920 925
Leu Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln
930 935 940
Met Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser
945 950 955 960
Leu Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser
965 970 975
Met Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile
980 985 990
Val Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp
995 1000 1005
Asn Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu
1010 1015 1020
Asn Lys Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His
1025 1030 1035
Asp Asn Ser Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys
1040 1045 1050
Asp Thr Leu Asp Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala
1055 1060 1065
Asp Asn Leu Phe Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp
1070 1075 1080
Val Thr Pro Ile Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu
1085 1090 1095
Trp Val Leu Asp Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly
1100 1105 1110
Lys Ala Ala Ala Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile
1115 1120 1125
Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp
1130 1135 1140
Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser
1145 1150 1155
Gly Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln
1160 1165 1170
Met Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe
1175 1180 1185
Thr Gly Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp
1190 1195 1200
Leu Tyr Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala
1205 1210 1215
Ser Gly Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys
1220 1225 1230
Tyr Ile His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala
1235 1240 1245
Gly Lys Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu
1250 1255 1260
Val Gly Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro
1265 1270 1275
Gln Ala Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn
1280 1285 1290
Trp Asn Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser Val
1295 1300 1305
Asn Pro Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe
1310 1315 1320
Ser Gly Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr
1325 1330 1335
Glu Phe Asp Leu Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala
1340 1345 1350
Ala Gly Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu
1355 1360 1365
Tyr Gly Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu
1370 1375 1380
Gly Leu Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp
1385 1390 1395
Asp Ala Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1400 1405
<210>27
<211>1183
<212>PRT
<213>大肠杆菌/智人
<400>27
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Asn Ser Asp Ser Glu Ser Pro Leu Ser
50 55 60
His Asp Gly Tyr Ser Leu His Asp Gly Val Ser Met Tyr Ile Glu Ala
65 70 75 80
Leu Asp Lys Tyr Ala Ser Asn Ser Val Val Gly Tyr Ile Gly Glu Arg
85 90 95
Ser Gln Tyr Arg Asp Leu Lys Trp Trp Glu Leu Arg Gly Ser Gly Ser
100 105 110
Gly Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly Gly Ala Leu
115 120 125
Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu Thr Gln Gly
130 135 140
Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp Leu Asn Ala
145 150 155 160
Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile Asn Leu Lys
165 170 175
Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg Asp Asn Tyr
180 185 190
Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala Gly Ile Val
195 200 205
Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly Val Lys Gly
210 215 220
Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val Gln Gly Thr
225 230 235 240
Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys Val Val Leu
245 250 255
Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe Ser Ser Val
260 265 270
Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp Glu Arg Gln
275 280 285
Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly Thr Leu Asp
290 295 300
Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala Ala Asp Tyr
305 310 315 320
Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr Ile Thr Leu
325 330 335
Asp Tyr Ala Leu Arg Ala Asp Lys Val Ala Leu Asn Gly Trp Ser Glu
340 345 350
Ser Gly Lys Gly Thr Ala Gly Asn Leu Tyr Lys Tyr Asn Asn Pro Tyr
355 360 365
Thr Asn Thr Thr Asp Tyr Phe Ile Leu Lys Gln Ser Thr Tyr Gly Tyr
370 375 380
Phe Pro Thr Asp Gln Ser Ser Asn Ala Thr Trp Glu Phe Val Gly His
385 390 395 400
Ser Gln Gly Asp Ala Gln Lys Leu Val Ala Asp Arg Phe Asn Thr Ala
405 410 415
Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp Asn
420 425 430
Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala Ala
435 440 445
Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu Gln
450 455 460
Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp Lys
465 470 475 480
Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser Phe
485 490 495
Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu Ser
500 505 510
Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn Thr
515 520 525
Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg Val
530 535 540
Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu Glu
545 550 555 560
Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe Asn Gly
565 570 575
Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile Phe
580 585 590
Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser Asp
595 600 605
Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn Leu
610 615 620
Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp Gly
625 630 635 640
Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro Asp
645 650 655
Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser Trp
660 665 670
Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser Met
675 680 685
Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu Gly
690 695 700
Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met Leu
705 710 715 720
Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu Asn
725 730 735
Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met Asn
740 745 750
Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val Gly
755 760 765
Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu
770 775 780
Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys Ala
785 790 795 800
Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser Ile
805 810 815
Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile
820 825 830
Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala
835 840 845
Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val
850 855 860
Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln Val
865 870 875 880
Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile
885 890 895
Ser Tyr Asn Asn Phe Ile Thr Glu Val Asn Asn Leu Asn Lys Arg Met
900 905 910
Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala Gly Thr Trp Val Arg Leu
915 920 925
Leu Asn Gly Ser Gly Ser Ala Asp Gly Gly Phe Thr Asp His Tyr Thr
930 935 940
Leu Leu Gln Met Gly Ala Asp Arg Lys His Glu Leu Gly Ser Met Asp
945 950 955 960
Leu Phe Thr Gly Val Met Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala
965 970 975
Asp Leu Tyr Ser Gly Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala
980 985 990
Ser Gly Leu Phe Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr
995 1000 1005
Ile His Asn Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly
1010 1015 1020
Lys Gln Asn Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val
1025 1030 1035
Gly Tyr Arg Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln
1040 1045 1050
Ala Glu Leu Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp
1055 1060 1065
Asn Asp Ser Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn
1070 1075 1080
Pro Leu Val Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser
1085 1090 1095
Gly Lys Asp Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu
1100 1105 1110
Phe Asp Leu Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala
1115 1120 1125
Gly Glu His Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr
1130 1135 1140
Gly Val Gly Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly
1145 1150 1155
Leu Glu Val Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp
1160 1165 1170
Ala Ile Asn Ala Asn Ile Arg Tyr Ser Phe
1175 1180
<210>28
<211>1360
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>28
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Phe Ser Arg Pro Gly Leu Pro Val Glu
50 55 60
Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp Ile Lys Val Gln
65 70 75 80
Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr Leu Leu Asp Gly
85 90 95
Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile Asn Thr Pro Ala
100 105 110
Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val Met Pro Val Gly
115 120 125
Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro Ala Cys Gly Lys
130 135 140
Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu Thr Ser Glu Leu
145 150 155 160
Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro Thr Gly Ser Ala
165 17 0175
Ala Ile Gly Leu Ser Gly Ser Gly Ser Gly Asn Asp Ala Pro Val Thr
180 185 190
Phe Arg Thr Ser Glu Gly Gly Ala Leu Glu Trp Ser Phe Asn Ser Ser
195 200 205
Thr Gly Ala Gly Ala Leu Thr Gln Gly Thr Thr Thr Tyr Ala Met His
210 215 220
Gly Gln Gln Gly Asn Asp Leu Asn Ala Gly Lys Asn Leu Ile Phe Gln
225 230 235 240
Gly Gln Asn Gly Gln Ile Asn Leu Lys Asp Ser Val Ser Gln Gly Ala
245 250 255
Gly Ser Leu Thr Phe Arg Asp Asn Tyr Thr Val Thr Thr Ser Asn Gly
260 265 270
Ser Thr Trp Thr Gly Ala Gly Ile Val Val Asp Asn Gly Val Ser Val
275 280 285
Asn Trp Gln Val Asn Gly Val Lys Gly Asp Asn Leu His Lys Ile Gly
290 295 300
Glu Gly Thr Leu Thr Val Gln Gly Thr Gly Ile Asn Glu Gly Gly Leu
305 310 315 320
Lys Val Gly Asp Gly Lys Val Val Leu Asn Gln Gln Ala Asp Asn Lys
325 330 335
Gly Gln Val Gln Ala Phe Ser Ser Val Asn Ile Ala Ser Gly Arg Pro
340 345 350
Thr Val Val Leu Thr Asp Glu Arg Gln Val Asn Pro Asp Thr Val Ser
355 360 365
Trp Gly Tyr Arg Gly Gly Thr Leu Asp Val Asn Gly Asn Ser Leu Thr
370 375 380
Phe His Gln Leu Lys Ala Ala Asp Tyr Gly Ala Val Leu Ala Asn Asn
385 390 395 400
Val Asp Lys Arg Ala Thr Ile Thr Leu Asp Tyr Ala Gly Ser Gly Ser
405 410 415
Ser Thr Gly Ser Ala Ala Ile Gly Leu Ser Met Ala Gly Ser Ser Ala
420 425 430
Met Ile Leu Ala Ala Tyr His Pro Gln Gln Phe Ile Tyr Ala Gly Ser
435 440 445
Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly Met Gly Pro Ser Leu Ile
450 455 460
Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr Lys Ala Ala Asp Met Trp
465 470 475 480
Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg Asn Asp Pro Thr Gln Gln
485 490 495
Ile Pro Lys Leu Val Ala Asn Asn Thr Arg Leu Trp Val Tyr Cys Gly
500 505 510
Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala Asn Ile Pro Ala Glu Phe
515 520 525
Leu Glu Asn Phe Val Arg Ser Ser Asn Leu Lys Phe Gln Asp Ala Tyr
530 535 540
Asn Ala Ala Gly Gly His Asn Ala Val Phe Asn Phe Pro Pro Asn Gly
545 550 555 560
Thr His Ser Trp Glu Tyr Trp Gly Ala Gln Leu Asn Ala Met Lys Gly
565 570 575
Asp Leu Gln Ser Ser Leu Gly Ala Gly Gly Ser Gly Ser Gly Asn Thr
580 585 590
Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp
595 600 605
Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala
610 615 620
Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu
625 630 635 640
Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp
645 650 655
Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser
660 665 670
Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu
6 75 680 685
Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn
690 695 700
Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg
705 710 715 720
Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu
725 730 735
Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe Asn
740 745 750
Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile
755 760 765
Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser
770 775 780
Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn
785 790 795 800
Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp
805 810 815
Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro
820 825 830
Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser
835 840 845
Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser
850 855 860
Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu
865 870 875 880
Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met
885 890 895
Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu
900 905 910
Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met
915 920 925
Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val
930 935 940
Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn
945 950 955 960
Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys
965 970 975
Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser
980 985 990
Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp
995 1000 1005
Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe
1010 1015 1020
Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile
1025 1030 1035
Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp
1040 1045 1050
Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala
1055 1060 1065
Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu Val Asn
1070 1075 1080
Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn Gly Glu
1085 1090 1095
Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp
1100 1105 1110
Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala Asp
1115 1120 1125
Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met
1130 1135 1140
Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly
1145 1150 1155
Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe
1160 1165 1170
Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn
1175 1180 1185
Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn
1190 1195 1200
Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg
1205 1210 1215
Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu
1220 1225 1230
Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser
1235 1240 1245
Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val
1250 1255 1260
Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp
1265 1270 1275
Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu
1280 1285 1290
Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His
1295 1300 1305
Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly
1310 1315 1320
Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val
1325 1330 1335
Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn
1340 1345 1350
Ala Asn Ile Arg Tyr Ser Phe
1355 1360
<210>29
<211>1360
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>29
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Phe Ser Arg Pro Gly Leu Pro Val Glu
50 55 60
Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp Ile Lys Val Gln
65 70 75 80
Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr Leu Leu Asp Gly
85 90 95
Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile Asn Thr Pro Ala
100 105 110
Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val Met Pro Val Gly
115 120 125
Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro Ala Cys Gly Lys
130 135 140
Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu Thr Ser Glu Leu
145 150 155 160
Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro Thr Gly Ser Ala
165 170 175
Ala Ile Gly Leu Ser Gly Ser Gly Ser Gly Asn Asp Ala Pro Val Thr
180 185 190
Phe Arg Thr Ser Glu Gly Gly Ala Leu Glu Trp Ser Phe Asn Ser Ser
195 200 205
Thr Gly Ala Gly Ala Leu Thr Gln Gly Thr Thr Thr Tyr Ala Met His
210 215 220
Gly Gln Gln Gly Asn Asp Leu Asn Ala Gly Lys Asn Leu Ile Phe Gln
225 230 235 240
Gly Gln Asn Gly Gln Ile Asn Leu Lys Asp Ser Val Ser Gln Gly Ala
245 250 255
Gly Ser Leu Thr Phe Arg Asp Asn Tyr Thr Val Thr Thr Ser Asn Gly
260 265 270
Ser Thr Trp Thr Gly Ala Gly Ile Val Val Asp Asn Gly Val Ser Val
275 280 285
Asn Trp Gln Val Asn Gly Val Lys Gly Asp Asn Leu His Lys Ile Gly
290 295 300
Glu Gly Thr Leu Thr Val Gln Gly Thr Gly Ile Asn Glu Gly Gly Leu
305 310 315 320
Lys Val Gly Asp Gly Lys Val Val Leu Asn Gln Gln Ala Asp Asn Lys
325 330 335
Gly Gln Val Gln Ala Phe Ser Ser Val Asn Ile Ala Ser Gly Arg Pro
340 345 350
Thr Val Val Leu Thr Asp Glu Arg Gln Val Asn Pro Asp Thr Val Ser
355 360 365
Trp Gly Tyr Arg Gly Gly Thr Leu Asp Val Asn Gly Asn Ser Leu Thr
370 375 380
Phe His Gln Leu Lys Ala Ala Asp Tyr Gly Ala Val Leu Ala Asn Asn
385 390 395 400
Val Asp Lys Arg Ala Thr Ile Thr Leu Asp Tyr Ala Gly Ser Gly Ser
405 410 415
Ser Thr Gly Ser Ala Ala Ile Gly Leu Ser Met Ala Gly Ser Ser Ala
420 425 430
Met Ile Leu Ala Ala Tyr His Pro Gln Gln Phe Ile Tyr Ala Gly Ser
435 440 445
Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly Met Gly Pro Ser Leu Ile
450 455 460
Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr Lys Ala Ala Asp Met Trp
465 470 475 480
Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg Asn Asp Pro Thr Gln Gln
485 490 495
Ile Pro Lys Leu Val Ala Asn Asn Thr Arg Leu Trp Val Tyr Cys Gly
500 505 510
Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala Asn Ile Pro Ala Glu Phe
515 520 525
Leu Glu Asn Phe Val Arg Ser Ser Asn Leu Lys Phe Gln Asp Ala Tyr
530 535 540
Asn Ala Ala Gly Gly His Asn Ala Val Phe Asn Phe Pro Pro Asn Gly
545 550 555 560
Thr His Ser Trp Glu Tyr Trp Gly Ala Gln Leu Asn Ala Met Lys Gly
565 570 575
Asp Leu Gln Ser Ser Leu Gly Ala Gly Gly Ser Gly Ser Gly Asn Thr
580 585 590
Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp
595 600 605
Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala
610 615 620
Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu
625 630 635 640
Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp
645 650 655
Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser
660 665 670
Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu
675 680 685
Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn
690 695 700
Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg
705 710 715 720
Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu
725 730 735
Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe Asn
740 745 750
Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile
755 760 765
Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser
770 775 780
Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn
785 790 795 800
Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp
805 810 815
Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro
820 825 830
Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser
835 840 845
Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser
850 855 860
Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu
865 870 875 880
Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met
885 890 895
Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu
900 905 910
Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met
915 920 925
Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val
930 935 940
Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn
945 950 955 960
Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys
965 970 975
Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser
980 985 990
Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp
995 1000 1005
Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe
1010 1015 1020
Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile
1025 1030 1035
Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp
1040 1045 1050
Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala
1055 1060 1065
Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu Val Gly
1070 1075 1080
Ser Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn Gly Glu
1085 1090 1095
Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp
1100 1105 1110
Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala Asp
1115 1120 1125
Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met
1130 1135 1140
Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly
1145 1150 1155
Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe
1160 1165 1170
Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn
1175 1180 1185
Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn
1190 1195 1200
Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg
1205 1210 1215
Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu
1220 1225 1230
Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser
1235 1240 1245
Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val
1250 1255 1260
Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp
1265 1270 1275
Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu
1280 1285 1290
Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His
1295 1300 1305
Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly
1310 1315 1320
Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val
1325 1330 1335
Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn
1340 1345 1350
Ala Asn Ile Arg Tyr Ser Phe
1355 1360
<210>30
<211>1360
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>30
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Thr Gly Ser Ala Ala Ile Gly Leu Ser
50 55 60
Met Ala Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln
65 70 75 80
Phe Ile Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly
85 90 95
Met Gly Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr
100 105 110
Lys Ala Ala Asp Met Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg
115 120 125
Asn Asp Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg
130 135 140
Leu Trp Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala
145 150 155 160
Asn Ile Pro Ala Glu Phe Leu Glu Asn Phe Val Arg Ser Ser Asn Leu
165 170 175
Lys Phe Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe
180 185 190
Asn Phe Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln
195 200 205
Leu Asn Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly Gly
210 215 220
Ser Gly Ser Gly Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly
225 230 235 240
Gly Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu
245 250 255
Thr Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp
260 265 270
Leu Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile
275 280 285
Asn Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg
290 295 300
Asp Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala
305 310 315 320
Gly Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly
325 330 335
Val Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val
340 345 350
Gln Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys
355 360 365
Val Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe
370 375 380
Ser Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp
385 390 395 400
Glu Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly
405 410 415
Thr Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala
420 425 430
Ala Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr
435 440 445
Ile Thr Leu Asp Tyr Ala Gly Ser Gly Ser Ser Phe Ser Arg Pro Gly
450 455 460
Leu Pro Val Glu Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp
465 470 475 480
Ile Lys Val Gln Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr
485 490 495
Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile
500 505 510
Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val
515 520 525
Met Pro Val Gly Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro
530 535 540
Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu
545 550 555 560
Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro
565 570 575
Thr Gly Ser Ala Ala Ile Gly Leu Ser Gly Ser Gly Ser Gly Asn Thr
580 585 590
Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp
595 600 605
Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala
610 615 620
Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu
625 630 635 640
Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp
645 650 655
Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser
660 665 670
Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu
675 680 685
Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn
690 695 700
Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg
705 710 715 720
Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu
725 730 735
Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe Asn
740 745 750
Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile
755 760 765
Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser
770 775 780
Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn
785 790 795 800
Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp
805 810 815
Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro
820 825 830
Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser
835 840 845
Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser
850 855 860
Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu
865 870 875 880
Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met
885 890 895
Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu
900 905 910
Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met
915 920 925
Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val
930 935 940
Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn
945 950 955 960
Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys
965 970 975
Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser
980 985 990
Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp
995 1000 1005
Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe
1010 1015 1020
Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile
1025 1030 1035
Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp
1040 1045 1050
Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala
1055 1060 1065
Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu Val Asn
1070 1075 1080
Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn Gly Glu
1085 1090 1095
Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp
1100 1105 1110
Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala Asp
1115 1120 1125
Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met
1130 1135 1140
Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly
1145 1150 1155
Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe
1160 1165 1170
Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn
1175 1180 1185
Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn
1190 1195 1200
Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg
1205 1210 1215
Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu
1220 1225 1230
Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser
1235 1240 1245
Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val
1250 1255 1260
Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp
1265 1270 1275
Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu
1280 1285 1290
Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His
1295 1300 1305
Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly
1310 1315 1320
Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val
1325 1330 1335
Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn
1340 1345 1350
Ala Asn Ile Arg Tyr Ser Phe
1355 1360
<210>31
<211>1360
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>31
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Thr Gly Ser Ala Ala Ile Gly Leu Ser
50 55 60
Met Ala Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln
65 70 75 80
Phe Ile Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly
85 90 95
Met Gly Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr
100 105 110
Lys Ala Ala Asp Met Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg
115 120 125
Asn Asp Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg
130 135 140
Leu Trp Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala
145 150 155 160
Asn Ile Pro Ala Glu Phe Leu Glu Asn Phe Val Arg Ser Ser Asn Leu
165 170 175
Lys Phe Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe
180 185 190
Asn Phe Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln
195 200 205
Leu Asn Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly Gly
210 215 220
Ser Gly Ser Gly Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly
225 230 235 240
Gly Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu
245 250 255
Thr Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp
260 265 270
Leu Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile
275 280 285
Asn Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg
290 295 300
Asp Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala
305 310 315 320
Gly Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly
325 330 335
Val Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val
340 345 350
Gln Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys
355 360 365
Val Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe
370 375 380
Ser Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp
385 390 395 400
Glu Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly
405 410 415
Thr Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala
420 425 430
Ala Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr
435 440 445
Ile Thr Leu Asp Tyr Ala Gly Ser Gly Ser Ser Phe Ser Arg Pro Gly
450 455 460
Leu Pro Val Glu Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp
465 470 475 480
Ile Lys Val Gln Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr
485 490 495
Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile
500 505 510
Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val
515 520 525
Met Pro Val Gly Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro
530 535 540
Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu
545 550 555 560
Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro
565 570 575
Thr Gly Ser Ala Ala Ile Gly Leu Ser Gly Ser Gly Ser Gly Asn Thr
580 585 590
Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp
595 600 605
Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala
610 615 620
Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu
625 630 635 640
Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp
645 650 655
Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser
660 665 670
Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu
675 680 685
Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn
690 695 700
Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg
705 710 715 720
Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu
725 730 735
Glu Gly Thr Ser Val Ala Thr Lys Asp Ala Asp Lys Ser Val Phe Asn
740 745 750
Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn Ile Asn Asp Ile
755 760 765
Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val Asn Ile Ser Ser
770 775 780
Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser Thr Ala Leu Asn
785 790 795 800
Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser Phe Val Ser Asp
805 810 815
Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu Asn Ser Arg Pro
820 825 830
Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp Tyr Ala Gly Ser
835 840 845
Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val Gly Pro Tyr Ser
850 855 860
Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly Thr Val Thr Leu
865 870 875 880
Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu Gln Asn Gln Met
885 890 895
Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp Ser Gly Ser Leu
900 905 910
Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp Thr Gln Trp Ser Met
915 920 925
Asn Gly Asn Ser Thr Ala Gly Asn Met Lys Leu Asn Arg Thr Ile Val
930 935 940
Gly Phe Asn Gly Gly Thr Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn
945 950 955 960
Leu Asp Ala Val Gln Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys
965 970 975
Ala Asp Lys Leu Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser
980 985 990
Ile Trp Val Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp
995 1000 1005
Ile Pro Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe
1010 1015 1020
Arg Ala Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile
1025 1030 1035
Leu Ser Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp
1040 1045 1050
Gly Tyr Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala
1055 1060 1065
Thr Phe Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu Val Gly
1070 1075 1080
Ser Leu Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn Gly Glu
1085 1090 1095
Ala Gly Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp
1100 1105 1110
Gly Gly Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala Asp
1115 1120 1125
Arg Lys His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met
1130 1135 1140
Ala Thr Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly
1145 1150 1155
Lys Thr Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe
1160 1165 1170
Arg Ser Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn
1175 1180 1185
Glu Asn Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn
1190 1195 1200
Phe Arg Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg
1205 1210 1215
Tyr His Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu
1220 1225 1230
Val Trp Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser
1235 1240 1245
Gly Met Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val
1250 1255 1260
Gly Arg Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp
1265 1270 1275
Trp Ser Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu
1280 1285 1290
Thr Asp Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His
1295 1300 1305
Gln Ile Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly
1310 1315 1320
Leu Asn Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val
1325 1330 1335
Glu Arg Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn
1340 1345 1350
Ala Asn Ile Arg Tyr Ser Phe
1355 1360
<210>32
<211>1460
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>32
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Thr Gly Ser Ala Ala Ile Gly Leu Ser
50 55 60
Met Ala Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln
65 70 75 80
Phe Ile Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly
85 90 95
Met Gly Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr
100 105 110
Lys Ala Ala Asp Met Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg
115 120 125
Asn Asp Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg
130 135 140
Leu Trp Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala
145 150 155 160
Asn Ile Pro Ala Glu Phe Leu Glu Asn Phe Val Arg Ser Ser Asn Leu
165 170 175
Lys Phe Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe
180 185 190
Asn Phe Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln
195 200 205
Leu Asn Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly Gly
210 215 220
Ser Gly Ser Gly Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly
225 230 235 240
Gly Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu
245 250 255
Thr Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp
260 265 270
Leu Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile
275 280 285
Asn Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg
290 295 300
Asp Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala
305 310 315 320
Gly Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly
325 330 335
Val Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val
340 345 350
Gln Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys
355 360 365
Val Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe
370 375 380
Ser Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp
385 390 395 400
Glu Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly
405 410 415
Thr Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala
420 425 430
Ala Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr
435 440 445
Ile Thr Leu Asp Tyr Ala Gly Ser Gly Ser Ser Phe Ser Arg Pro Gly
450 455 460
Leu Pro Val Glu Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp
465 470 475 480
Ile Lys Val Gln Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr
485 490 495
Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile
500 505 510
Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val
515 520 525
Met Pro Val Gly Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro
530 535 540
Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu
545 550 555 560
Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro
565 570 575
Thr Gly Ser Ala Ala Ile Gly Leu Ser Gly Ser Gly Ser Gly Asn Thr
580 585 590
Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp
595 600 605
Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala
610 615 620
Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu
625 630 635 640
Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp
645 650 655
Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser
660 665 670
Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu
675 680 685
Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn
690 695 700
Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg
705 710 715 720
Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu
725 730 735
Glu Gly Thr Ser Val Ala Gly Ser Gly Ser Ser Met Thr Glu Gln Gln
740 745 750
Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn
755 760 765
Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr
770 775 780
Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly
785 790 795 800
Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu
805 810 815
Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser
820 825 830
Thr Glu Gly Asn Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Lys
835 840 845
Ser Val Phe Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn
850 855 860
Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val
865 870 875 880
Asn Ile Ser Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser
885 890 895
Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser
900 905 910
Phe Val Ser Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu
915 920 925
Asn Ser Arg Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp
930 935 940
Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val
945 950 955 960
Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly
965 970 975
Thr Val Thr Leu Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu
980 985 990
Gln Asn Gln Met Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp
995 1000 1005
Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp
1010 1015 1020
Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly Asn Met Lys
1025 1030 1035
Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr Ser Pro Phe
1040 1045 1050
Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser Ala Phe
1055 1060 1065
Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val Ile Asn
1070 1075 1080
Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe Leu
1085 1090 1095
Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser
1100 1105 1110
Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg
1115 1120 1125
Val Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys
1130 1135 1140
Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala
1145 1150 1155
Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile
1160 1165 1170
Ser Tyr Asn Asn Phe Ile Thr Glu Val Asn Asn Leu Asn Lys Arg
1175 1180 1185
Met Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala Gly Thr Trp Val
1190 1195 1200
Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp Gly Gly Phe Thr Asp
1205 1210 1215
His Tyr Thr Leu Leu Gln Met Gly Ala Asp Arg Lys His Glu Leu
1220 1225 1230
Gly Ser Met Asp Leu Phe Thr Gly Val Met Ala Thr Tyr Thr Asp
1235 1240 1245
Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly Lys Thr Lys Ser Trp
1250 1255 1260
Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe Arg Ser Gly Ala Tyr
1265 1270 1275
Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu Asn Lys Tyr Asp
1280 1285 1290
Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg Ser His Ser
1295 1300 1305
Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His Leu Thr Asp
1310 1315 1320
Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp Gly Arg Leu
1325 1330 1335
Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met Asp Val Ser
1340 1345 1350
Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg Thr Gly Val
1355 1360 1365
Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser Leu Thr Ala
1370 1375 1380
Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp Ser Ala Asp
1385 1390 1395
Val His Leu Lys Asp Ala Ala Gly Glu His Gln Ile Asn Gly Arg
1400 1405 1410
Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu Asn Ala Arg Phe
1415 1420 1425
Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu Arg Ser Ala Phe
1430 1435 1440
Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn Ile Arg Tyr
1445 1450 1455
Ser Phe
1460
<210>33
<211>1460
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>33
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Thr Gly Ser Ala Ala Ile Gly Leu Ser
50 55 60
Met Ala Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln
65 70 75 80
Phe Ile Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly
85 90 95
Met Gly Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr
100 105 110
Lys Ala Ala Asp Met Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg
115 120 125
Asn Asp Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg
130 135 140
Leu Trp Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala
145 150 155 160
Asn Ile Pro Ala Glu Phe Leu Glu Asn Phe Val Arg Ser Ser Asn Leu
165 170 175
Lys Phe Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe
180 185 190
Asn Phe Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln
195 200 205
Leu Asn Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly Gly
210 215 220
Ser Gly Ser Gly Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly
225 230 235 240
Gly Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu
245 250 255
Thr Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp
260 265 270
Leu Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile
275 280 285
Asn Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg
290 295 300
Asp Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala
305 310 315 320
Gly Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly
325 330 335
Val Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val
340 345 350
Gln Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys
355 360 365
Val Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe
370 375 380
Ser Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp
385 390 395 400
Glu Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly
405 410 415
Thr Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala
420 425 430
Ala Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr
435 440 445
Ile Thr Leu Asp Tyr Ala Gly Ser Gly Ser Ser Phe Ser Arg Pro Gly
450 455 460
Leu Pro Val Glu Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp
465 470 475 480
Ile Lys Val Gln Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr
485 490 495
Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile
500 505 510
Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val
515 520 525
Met Pro Val Gly Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro
530 535 540
Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu
545 550 555 560
Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro
565 570 575
Thr Gly Ser Ala Ala Ile Gly Leu Ser Gly Ser Gly Ser Gly Asn Thr
580 585 590
Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp
595 600 605
Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala
610 615 620
Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu
625 630 635 640
Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp
645 650 655
Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser
660 665 670
Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu
675 680 685
Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn
690 695 700
Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg
705 710 715 720
Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu
725 730 735
Glu Gly Thr Ser Val Ala Gly Ser Gly Ser Ser Met Thr Glu Gln Gln
740 745 750
Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn
755 760 765
Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr
770 775 780
Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly
785 790 795 800
Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu
805 810 815
Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser
820 825 830
Thr Glu Gly Asn Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Lys
835 840 845
Ser Val Phe Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn
850 855 860
Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val
865 870 875 880
Asn Ile Ser Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser
885 890 895
Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser
900 905 910
Phe Val Ser Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu
915 920 925
Asn Ser Arg Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp
930 935 940
Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val
945 950 955 960
Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly
965 970 975
Thr Val Thr Leu Gly Gly Glu Gly Glu Leu Ser Pro Asp Leu Thr Leu
980 985 990
Gln Asn Gln Met Leu Tyr Ser Leu Phe Asn Gly Tyr Arg Asn Ile Trp
995 1000 1005
Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val Ser Met Thr Asp
1010 1015 1020
Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly Asn Met Lys
1025 1030 1035
Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr Ser Pro Phe
1040 1045 1050
Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln Ser Ala Phe
1055 1060 1065
Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu Val Ile Asn
1070 1075 1080
Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val Asn Phe Leu
1085 1090 1095
Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro Leu Val Ser
1100 1105 1110
Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala Ser Thr Arg
1115 1120 1125
Val Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser Val Arg Lys
1130 1135 1140
Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr Gln Val Ala
1145 1150 1155
Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe Met His Ile
1160 1165 1170
Ser Tyr Asn Asn Phe Ile Thr Glu Val Gly Ser Leu Asn Lys Arg
1175 1180 1185
Met Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala Gly Thr Trp Val
1190 1195 1200
Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp Gly Gly Phe Thr Asp
1205 1210 1215
His Tyr Thr Leu Leu Gln Met Gly Ala Asp Arg Lys His Glu Leu
1220 1225 1230
Gly Ser Met Asp Leu Phe Thr Gly Val Met Ala Thr Tyr Thr Asp
1235 1240 1245
Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly Lys Thr Lys Ser Trp
1250 1255 1260
Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe Arg Ser Gly Ala Tyr
1265 1270 1275
Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu Asn Lys Tyr Asp
1280 1285 1290
Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg Ser His Ser
1295 1300 1305
Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His Leu Thr Asp
1310 1315 1320
Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp Gly Arg Leu
1325 1330 1335
Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met Asp Val Ser
1340 1345 1350
Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg Thr Gly Val
1355 1360 1365
Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser Leu Thr Ala
1370 1375 1380
Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp Ser Ala Asp
1385 1390 1395
Val His Leu Lys Asp Ala Ala Gly Glu His Gln Ile Asn Gly Arg
1400 1405 1410
Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu Asn Ala Arg Phe
1415 1420 1425
Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu Arg Ser Ala Phe
1430 1435 1440
Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn Ile Arg Tyr
1445 1450 1455
Ser Phe
1460
<210>34
<211>1523
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>34
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Thr Gly Ser Ala Ala Ile Gly Leu Ser
50 55 60
Met Ala Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln
65 70 75 80
Phe Ile Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly
85 90 95
Met Gly Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr
100 105 110
Lys Ala Ala Asp Met Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg
115 120 125
Asn Asp Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg
130 135 140
Leu Trp Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala
145 150 155 160
Asn Ile Pro Ala Glu Phe Leu Glu Asn Phe Val Arg Ser Ser Asn Leu
165 170 175
Lys Phe Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe
180 185 190
Asn Phe Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln
195 200 205
Leu Asn Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly Gly
210 215 220
Ser Gly Ser Gly Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly
225 230 235 240
Gly Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu
245 250 255
Thr Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp
260 265 270
Leu Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile
275 280 285
Asn Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg
290 295 300
Asp Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala
305 310 315 320
Gly Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly
325 330 335
Val Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val
340 345 350
Gln Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys
355 360 365
Val Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe
370 375 380
Ser Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp
385 390 395 400
Glu Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly
405 410 415
Thr Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala
420 425 430
Ala Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr
435 440 445
Ile Thr Leu Asp Tyr Ala Gly Ser Gly Ser Ser Phe Ser Arg Pro Gly
450 455 460
Leu Pro Val Glu Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp
465 470 475 480
Ile Lys Val Gln Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr
485 490 495
Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile
500 505 510
Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val
515 520 525
Met Pro Val Gly Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro
530 535 540
Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu
545 550 555 560
Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro
565 570 575
Thr Gly Ser Ala Ala Ile Gly Leu Ser Gly Ser Gly Ser Gly Asn Thr
580 585 590
Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp
595 600 605
Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala
610 615 620
Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu
625 630 635 640
Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp
645 650 655
Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser
660 665 670
Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu
675 680 685
Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn
690 695 700
Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg
705 710 715 720
Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu
725 730 735
Glu Gly Thr Ser Val Ala Gly Ser Gly Ser Ser Met Thr Glu Gln Gln
740 745 750
Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn
755 760 765
Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr
770 775 780
Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly
785 790 795 800
Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu
805 810 815
Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser
820 825 830
Thr Glu Gly Asn Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Lys
835 840 845
Ser Val Phe Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn
850 855 860
Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val
865 870 875 880
Asn Ile Ser Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser
885 890 895
Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser
900 905 910
Phe Val Ser Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu
915 920 925
Asn Ser Arg Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp
930 935 940
Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val
945 950 955 960
Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly
965 970 975
Thr Val Thr Leu Gly Gly Ser Gly Ser Ser Val Ile Ala Gly Val Asp
980 985 990
Gln Ala Leu Ala Ala Thr Gly Gln Ala Ser Gln Arg Ala Ala Gly Ala
995 1000 1005
Ser Gly Gly Val Thr Val Gly Val Gly Val Gly Thr Glu Gln Arg
1010 1015 1020
Asn Leu Ser Val Val Ala Pro Ser Gln Phe Thr Phe Ser Ser Arg
1025 1030 1035
Ser Pro Asp Phe Val Asp Glu Thr Ala Gly Gln Ser Trp Cys Ala
1040 1045 1050
Ile Leu Gly Leu Asn Gln Phe His Gly Ser Gly Ser Gly Tyr Arg
1055 1060 1065
Asn Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val Ser
1070 1075 1080
Met Thr Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly
1085 1090 1095
Asn Met Lys Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr
1100 1105 1110
Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln
1115 1120 1125
Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu
1130 1135 1140
Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val
1145 1150 1155
Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro
1160 1165 1170
Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala
1175 1180 1185
Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser
1190 1195 1200
Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr
1205 1210 1215
Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe
1220 1225 1230
Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu Val Asn Asn Leu
1235 1240 1245
Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala Gly
1250 1255 1260
Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp Gly Gly
1265 1270 1275
Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala Asp Arg Lys
1280 1285 1290
His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met Ala Thr
1295 1300 1305
Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly Lys Thr
1310 1315 1320
Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe Arg Ser
1325 1330 1335
Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu Asn
1340 1345 1350
Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg
1355 1360 1365
Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His
1370 1375 1380
Leu ThrA sp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp
1385 1390 1395
Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met
1400 1405 1410
Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg
1415 1420 1425
Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser
1430 1435 1440
Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp
1445 1450 1455
Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His Gln Ile
1460 1465 1470
Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu Asn
1475 1480 1485
Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu Arg
1490 1495 1500
Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn
1505 1510 1515
Ile Arg Tyr Ser Phe
1520
<210>35
<211>1523
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>35
Met Asn Arg Ile Tyr Ser Leu Arg Tyr Ser Ala Val Ala Arg Gly Phe
1 5 10 15
Ile Ala Val Ser Glu Phe Ala Arg Lys Cys Val His Lys Ser Val Arg
20 25 30
Arg Leu Cys Phe Pro Val Leu Leu Leu Ile Pro Val Leu Phe Ser Ala
35 40 45
Gly Ser Leu Ala Gly Ser Ser Thr Gly Ser Ala Ala Ile Gly Leu Ser
50 55 60
Met Ala Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln
65 70 75 80
Phe Ile Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly
85 90 95
Met Gly Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr
100 105 110
Lys Ala Ala Asp Met Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg
115 120 125
Asn Asp Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg
130 135 140
Leu Trp Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala
145 150 155 160
Asn Ile Pro Ala Glu Phe Leu Glu Asn Phe Val Arg Ser Ser Asn Leu
165 170 175
Lys Phe Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe
180 185 190
Asn Phe Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln
195 200 205
Leu Asn Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly Gly
210 215 220
Ser Gly Ser Gly Asn Asp Ala Pro Val Thr Phe Arg Thr Ser Glu Gly
225 230 235 240
Gly Ala Leu Glu Trp Ser Phe Asn Ser Ser Thr Gly Ala Gly Ala Leu
245 250 255
Thr Gln Gly Thr Thr Thr Tyr Ala Met His Gly Gln Gln Gly Asn Asp
260 265 270
Leu Asn Ala Gly Lys Asn Leu Ile Phe Gln Gly Gln Asn Gly Gln Ile
275 280 285
Asn Leu Lys Asp Ser Val Ser Gln Gly Ala Gly Ser Leu Thr Phe Arg
290 295 300
Asp Asn Tyr Thr Val Thr Thr Ser Asn Gly Ser Thr Trp Thr Gly Ala
305 310 315 320
Gly Ile Val Val Asp Asn Gly Val Ser Val Asn Trp Gln Val Asn Gly
325 330 335
Val Lys Gly Asp Asn Leu His Lys Ile Gly Glu Gly Thr Leu Thr Val
340 345 350
Gln Gly Thr Gly Ile Asn Glu Gly Gly Leu Lys Val Gly Asp Gly Lys
355 360 365
Val Val Leu Asn Gln Gln Ala Asp Asn Lys Gly Gln Val Gln Ala Phe
370 375 380
Ser Ser Val Asn Ile Ala Ser Gly Arg Pro Thr Val Val Leu Thr Asp
385 390 395 400
Glu Arg Gln Val Asn Pro Asp Thr Val Ser Trp Gly Tyr Arg Gly Gly
405 410 415
Thr Leu Asp Val Asn Gly Asn Ser Leu Thr Phe His Gln Leu Lys Ala
420 425 430
Ala Asp Tyr Gly Ala Val Leu Ala Asn Asn Val Asp Lys Arg Ala Thr
435 440 445
Ile Thr Leu Asp Tyr Ala Gly Ser Gly Ser Ser Phe Ser Arg Pro Gly
450 455 460
Leu Pro Val Glu Tyr Leu Gln Val Pro Ser Pro Ser Met Gly Arg Asp
465 470 475 480
Ile Lys Val Gln Phe Gln Ser Gly Gly Asn Asn Ser Pro Ala Val Tyr
485 490 495
Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp Tyr Asn Gly Trp Asp Ile
500 505 510
Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln Ser Gly Leu Ser Ile Val
515 520 525
Met Pro Val Gly Gly Gln Ser Ser Phe Tyr Ser Asp Trp Tyr Ser Pro
530 535 540
Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr Lys Trp Glu Thr Phe Leu
545 550 555 560
Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala Asn Arg Ala Val Lys Pro
565 570 575
Thr Gly Ser Ala Ala Ile Gly Leu Ser Gly Ser Gly Ser Gly Asn Thr
580 585 590
Ala Gly Tyr Leu Phe His Gly Gln Leu Lys Gly Asn Leu Asn Val Asp
595 600 605
Asn Arg Leu Pro Glu Gly Val Thr Gly Ala Leu Val Met Asp Gly Ala
610 615 620
Ala Asp Ile Ser Gly Thr Phe Thr Gln Glu Asn Gly Arg Leu Thr Leu
625 630 635 640
Gln Gly His Pro Val Ile His Ala Tyr Asn Thr Gln Ser Val Ala Asp
645 650 655
Lys Leu Ala Ala Ser Gly Asp His Ser Val Leu Thr Gln Pro Thr Ser
660 665 670
Phe Ser Gln Glu Asp Trp Glu Asn Arg Ser Phe Thr Phe Asp Arg Leu
675 680 685
Ser Leu Lys Asn Thr Asp Phe Gly Leu Gly Arg Asn Ala Thr Leu Asn
690 695 700
Thr Thr Ile Gln Ala Asp Asn Ser Ser Val Thr Leu Gly Asp Ser Arg
705 710 715 720
Val Phe Ile Asp Lys Asn Asp Gly Gln Gly Thr Ala Phe Thr Leu Glu
725 730 735
Glu Gly Thr Ser Val Ala Gly Ser Gly Ser Ser Met Thr Glu Gln Gln
740 745 750
Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn
755 760 765
Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr
770 775 780
Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly
785 790 795 800
Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu
805 810 815
Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser
820 825 830
Thr Glu Gly Asn Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Lys
835 840 845
Ser Val Phe Asn Gly Thr Val Asn Leu Asp Asn Gln Ser Val Leu Asn
850 855 860
Ile Asn Asp Ile Phe Asn Gly Gly Ile Gln Ala Asn Asn Ser Thr Val
865 870 875 880
Asn Ile Ser Ser Asp Ser Ala Val Leu Gly Asn Ser Thr Leu Thr Ser
885 890 895
Thr Ala Leu Asn Leu Asn Lys Gly Ala Asn Ala Leu Ala Ser Gln Ser
900 905 910
Phe Val Ser Asp Gly Pro Val Asn Ile Ser Asp Ala Thr Leu Ser Leu
915 920 925
Asn Ser Arg Pro Asp Glu Val Ser His Thr Leu Leu Pro Val Tyr Asp
930 935 940
Tyr Ala Gly Ser Trp Asn Leu Lys Gly Asp Asp Ala Arg Leu Asn Val
945 950 955 960
Gly Pro Tyr Ser Met Leu Ser Gly Asn Ile Asn Val Gln Asp Lys Gly
965 970 975
Thr Val Thr Leu Gly Gly Ser Gly Ser Ser Val Ile Ala Gly Val Asp
980 985 990
Gln Ala Leu Ala Ala Thr Gly Gln Ala Ser Gln Arg Ala Ala Gly Ala
995 1000 1005
Ser Gly Gly Val Thr Val Gly Val Gly Val Gly Thr Glu Gln Arg
1010 1015 1020
Asn Leu Ser Val Val Ala Pro Ser Gln Phe Thr Phe Ser Ser Arg
1025 1030 1035
Ser Pro Asp Phe Val Asp Glu Thr Ala Gly Gln Ser Trp Cys Ala
1040 1045 1050
Ile Leu Gly Leu Asn Gln Phe His Gly Ser Gly Ser Gly Tyr Arg
1055 1060 1065
Asn Ile Trp Ser Gly Ser Leu Asn Ala Pro Asp Ala Thr Val Ser
1070 1075 1080
Met Thr Asp Thr Gln Trp Ser Met Asn Gly Asn Ser Thr Ala Gly
1085 1090 1095
Asn Met Lys Leu Asn Arg Thr Ile Val Gly Phe Asn Gly Gly Thr
1100 1105 1110
Ser Pro Phe Thr Thr Leu Thr Thr Asp Asn Leu Asp Ala Val Gln
1115 1120 1125
Ser Ala Phe Val Met Arg Thr Asp Leu Asn Lys Ala Asp Lys Leu
1130 1135 1140
Val Ile Asn Lys Ser Ala Thr Gly His Asp Asn Ser Ile Trp Val
1145 1150 1155
Asn Phe Leu Lys Lys Pro Ser Asn Lys Asp Thr Leu Asp Ile Pro
1160 1165 1170
Leu Val Ser Ala Pro Glu Ala Thr Ala Asp Asn Leu Phe Arg Ala
1175 1180 1185
Ser Thr Arg Val Val Gly Phe Ser Asp Val Thr Pro Ile Leu Ser
1190 1195 1200
Val Arg Lys Glu Asp Gly Lys Lys Glu Trp Val Leu Asp Gly Tyr
1205 1210 1215
Gln Val Ala Arg Asn Asp Gly Gln Gly Lys Ala Ala Ala Thr Phe
1220 1225 1230
Met His Ile Ser Tyr Asn Asn Phe Ile Thr Glu Val Gly Ser Leu
1235 1240 1245
Asn Lys Arg Met Gly Asp Leu Arg Asp Ile Asn Gly Glu Ala Gly
1250 1255 1260
Thr Trp Val Arg Leu Leu Asn Gly Ser Gly Ser Ala Asp Gly Gly
1265 1270 1275
Phe Thr Asp His Tyr Thr Leu Leu Gln Met Gly Ala Asp Arg Lys
1280 1285 1290
His Glu Leu Gly Ser Met Asp Leu Phe Thr Gly Val Met Ala Thr
1295 1300 1305
Tyr Thr Asp Thr Asp Ala Ser Ala Asp Leu Tyr Ser Gly Lys Thr
1310 1315 1320
Lys Ser Trp Gly Gly Gly Phe Tyr Ala Ser Gly Leu Phe Arg Ser
1325 1330 1335
Gly Ala Tyr Phe Asp Val Ile Ala Lys Tyr Ile His Asn Glu Asn
1340 1345 1350
Lys Tyr Asp Leu Asn Phe Ala Gly Ala Gly Lys Gln Asn Phe Arg
1355 1360 1365
Ser His Ser Leu Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His
1370 1375 1380
Leu Thr Asp Thr Thr Phe Val Glu Pro Gln Ala Glu Leu Val Trp
1385 1390 1395
Gly Arg Leu Gln Gly Gln Thr Phe Asn Trp Asn Asp Ser Gly Met
1400 1405 1410
Asp Val Ser Met Arg Arg Asn Ser Val Asn Pro Leu Val Gly Arg
1415 1420 1425
Thr Gly Val Val Ser Gly Lys Thr Phe Ser Gly Lys Asp Trp Ser
1430 1435 1440
Leu Thr Ala Arg Ala Gly Leu His Tyr Glu Phe Asp Leu Thr Asp
1445 1450 1455
Ser Ala Asp Val His Leu Lys Asp Ala Ala Gly Glu His Gln Ile
1460 1465 1470
Asn Gly Arg Lys Asp Ser Arg Met Leu Tyr Gly Val Gly Leu Asn
1475 1480 1485
Ala Arg Phe Gly Asp Asn Thr Arg Leu Gly Leu Glu Val Glu Arg
1490 1495 1500
Ser Ala Phe Gly Lys Tyr Asn Thr Asp Asp Ala Ile Asn Ala Asn
1505 1510 1515
Ile Arg Tyr Ser Phe
1520
<210>36
<211>1306
<212>PRT
<213>大肠杆菌
<400>36
Met Asn Lys Ile Tyr Ala Leu Lys Tyr Cys His Ala Thr Gly Gly Leu
1 5 10 15
Ile Ala Val Ser Glu Leu Ala Ser Arg Val Met Lys Lys Ala Ala Arg
20 25 30
Gly Ser Leu Leu Ala Leu Phe Asn Leu Ser Leu Tyr Gly Ala Phe Leu
35 40 45
Ser Ala Ser Gln Ala Ala Gln Leu Asn Ile Asp Asn Val Trp Ala Arg
50 55 60
Asp Tyr Leu Asp Leu Ala Gln Asn Lys Gly Val Phe Lys Ala Gly Ala
65 70 75 80
Thr Asn Val Ser Ile Gln Leu Lys Asn Gly Gln Thr Phe Asn Phe Pro
85 90 95
Asn Val Pro Ile Pro Asp Phe Ser Pro Ala Ser Asn Lys Gly Ala Thr
100 105 110
Thr Ser Ile Gly Gly Ala Tyr Ser Val Thr Ala Thr His Asn Gly Thr
115 120 125
Thr His His Ala Ile Ser Thr Gln Asn Trp Gly Gln Ser Ser Tyr Lys
130 135 140
Tyr Ile Asp Arg Met Thr Asn Gly Asp Phe Ala Val Thr Arg Leu Asp
145 150 155 160
Lys Phe Val Val Glu Thr Thr Gly Val Lys Asn Ser Val Asp Phe Ser
165 170 175
Leu Asn Ser His Asp Ala Leu Glu Arg Tyr Gly Val Glu Ile Asn Gly
180 185 190
Glu Lys Lys Ile Ile Gly Phe Arg Val Gly Ala Gly Thr Thr Tyr Thr
195 200 205
Ala Gln Asn Gly Asn Thr Tyr Ser Thr Gly Gln Val Tyr Asn Pro Leu
210 215 220
Leu Leu Ser Ala Ser Met Phe Gln Leu Asn Trp Asp Asn Lys Arg Pro
225 230 235 240
Tyr Asn Asn Thr Thr Pro Phe Tyr Asn Glu Thr Thr Gly Gly Asp Ser
245 250 255
Gly Ser Gly Phe Tyr Leu Tyr Asp Asn Val Lys Lys Glu Trp Val Met
260 265 270
Leu Gly Thr Leu Phe Gly Ile Ala Ser Ser Gly Ala Asp Val Gly Ser
275 280 285
Ile Leu Asn Gln Tyr Asp Glu Asn Thr Val Asn Gly Leu Lys Asn Lys
290 295 300
Phe Thr Gln Lys Val Gln Leu Asn Asn Asn Thr Met Ser Leu Asn Ser
305 310 315 320
Asp Ser Phe Thr Leu Ala Gly Asn Asn Thr Ala Val Ala Lys Asn Asn
325 330 335
Asn Asn Tyr Lys Asp Leu Ser Phe Ser Gly Gly Gly Ser Ile Asn Phe
340 345 350
Asp Asn Asp Val Asn Ile Gly Ser Gly Gly Leu Ile Phe Asp Ala Gly
355 360 365
His His Tyr Thr Val Thr Gly Asn Asn Lys Thr Phe Lys Gly Ala Gly
370 375 380
Leu Asp Ile Gly Asp Asn Thr Thr Val Asp Trp Asn Val Lys Gly Val
385 390 395 400
Val Gly Asp Asn Leu His Lys Ile Gly Ala Gly Thr Leu Asn Val Asn
405 410 415
Val Ser Gln Gly Asn Asn Leu Lys Thr Gly Asp Gly Leu Val Val Leu
420 425 430
Asn Ser Ala Asn Ala Phe Asp Asn Ile Tyr Met Ala Ser Gly His Gly
435 440 445
Val Val Lys Ile Asn His Ser Ala Ala Leu Asn Gln Asn Asn Asp Tyr
450 455 460
Arg Gly Ile Phe Phe Thr Glu Asn Gly Gly Thr Leu Asp Leu Asn Gly
465 470 475 480
Tyr Asp Gln Ser Phe Asn Lys Ile Ala Ala Thr Asp Ile Gly Ala Leu
485 490 495
Ile Thr Asn Ser Ala Val Gln Lys Ala Val Leu Ser Val Asn Asn Gln
500 505 510
Ser His Tyr Met Tyr His Gly Ser Val Ser Gly Asn Thr Glu Ile Asn
515 520 525
His Gln Phe Asp Thr Gln Lys Asn Asn Ser Arg Leu Ile Leu Asp Gly
530 535 540
Asn Val Asp Ile Thr Asn Asp Ile Asn Ile Lys Asn Ser Gln Leu His
545 550 555 560
His Ala Gly Thr Cys Leu His Leu Met Leu Phe Leu Glu Arg Val Gly
565 570 575
Val Thr Cys Met Leu Pro Gly Val Ile Cys Glu Lys Asp Tyr Val Ser
580 585 590
Gly Ile Gln Gln Gln Glu Asn Ser Ala Asn Lys Asn Asn Asn Thr Asp
595 600 605
Tyr Lys Thr Asn Asn Gln Val Ser Ser Phe Glu Gln Pro Asp Trp Glu
610 615 620
Asn Arg Leu Phe Lys Phe Lys Thr Leu Asn Leu Ile Asn Ser Asp Phe
625 630 635 640
Ile Val Gly Arg Asn Ala Ile Val Val Gly Asp Ile Ser Ala Asn Asn
645 650 655
Ser Thr Leu Ser Leu Ser Gly Lys Asp Thr Lys Val His Ile Asp Met
660 665 670
Tyr Asp Gly Lys Asn Ile Thr Gly Asp Gly Phe Gly Phe Arg Gln Asp
675 680 685
Ile Lys Asp Gly Val Ser Val Ser Pro Glu Ser Ser Ser Tyr Phe Gly
690 695 700
Asn Val Thr Leu Asn Asn His Ser Leu Leu Asp Ile Gly Asn Lys Phe
705 710 715 720
Thr Gly Gly Ile Glu Ala Tyr Asp Ser Ser Val Ser Val Thr Ser Gln
725 730 735
Asn Ala Val Phe Asp Arg Val Gly Ser Phe Val Asn Ser Ser Leu Thr
740 745 750
Leu Glu Lys Gly Ala Lys Leu Thr Ala Gln Gly Gly Ile Phe Ser Thr
755 760 765
Gly Ala Val Asp Val Lys Glu Asn Ala Ser Leu Ile Leu Thr Gly Thr
770 775 780
Pro Ser Ala Gln Lys Gln Glu Tyr Tyr Ser Pro Val Ile Ser Thr Thr
785 790 795 800
Glu Gly Ile Asn Leu Gly Asp Lys Ala Ser Leu Ser Val Lys Asn Met
805 810 815
Gly Tyr Leu Ser Ser Asp Ile His Ala Gly Thr Thr Ala Ala Thr Ile
820 825 830
Asn Leu Gly Asp Gly Asp Ala Glu Thr Asp Ser Pro Leu Phe Ser Ser
835 840 845
Leu Ile Arg Gly Tyr Asn Ala Val Leu Ser Gly Asn Ile Thr Gly Glu
850 855 860
Gln Ser Thr Val Asn Met Asn Asn Ala Leu Trp Tyr Ser Asp Gly Asn
865 870 875 880
Ser Thr Ile Gly Thr Leu Lys Ser Thr Gly Gly Arg Val Glu Leu Gly
885 890 895
Gly Gly Lys Asp Phe Ala Thr Leu Arg Val Lys Glu Leu Asn Ala Asn
900 905 910
Asn Ala Thr Phe Leu Met His Thr Asn Thr Ser Gln Ala Asp Gln Leu
915 920 925
Asn Val Thr Asn Lys Leu Leu Gly Ser Asn Asn Thr Val Leu Val Asp
9 30 935 940
Phe Leu Asn Lys Pro Ala Ser Glu Met Asn Val Thr Leu Ile Thr Ala
945 950 955 960
Pro Lys Gly Ser Asp Glu Lys Thr Phe Thr Ala Gly Thr Gln Gln Ile
965 970 975
Gly Phe Ser Asn Val Thr Pro Val Ile Ser Thr Glu Lys Thr Asp Asp
980 985 990
Ala Thr Lys Trp Met Leu Thr Gly Tyr Gln Thr Val Ser Asp Ala Gly
995 1000 1005
Ala Ser Lys Thr Ala Thr Asp Phe Met Ala Ser Gly Tyr Lys Ser
1010 1015 1020
Phe Leu Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu
1025 1030 1035
Arg Asp Thr Gln Gly Asp Ala Gly Val Trp Ala Arg Ile Met Asn
1040 1045 1050
Gly Thr Gly Ser Ala Asp Gly Gly Tyr Ser Asp Asn Tyr Thr His
1055 1060 1065
Val Gln Ile Gly Ala Asp Arg Lys His Glu Leu Asp Gly Val Asp
1070 1075 1080
Leu Phe Thr Gly Ala Leu Leu Thr Tyr Thr Asp Ser Asn Ala Ser
1085 1090 1095
Ser His Ala Phe Ser Gly Lys Thr Lys Ser Val Gly Gly Gly Leu
1100 1105 1110
Tyr Ala Ser Ala Leu Phe Asp Ser Gly Ala Tyr Phe Asp Leu Ile
1115 1120 1125
Gly Lys Tyr Leu His His Asp Asn Gln Tyr Thr Ala Ser Phe Ala
1130 1135 1140
Ser Leu Gly Thr Lys Asp Tyr Ser Ser His Ser Trp Tyr Ala Gly
1145 1150 1155
Ala Glu Val Gly Tyr Arg Tyr His Leu Ser Glu Glu Ser Trp Val
1160 1165 1170
Glu Pro Gln Met Glu Leu Val Tyr Gly Ser Val Ser Gly Lys Ser
1175 1180 1185
Phe Ile Trp Glu Asp Arg Gly Met Ala Leu Ser Met Lys Asp Lys
1190 1195 1200
Asp Tyr Asn Pro Leu Ile Gly Arg Thr Gly Val Asp Val Gly Arg
1205 1210 1215
Thr Phe Ser Gly Asp Asp Trp Lys Ile Thr Ala Arg Ala Gly Leu
1220 1225 1230
Gly Tyr Gln Phe Asp Leu Leu Ala Asn Gly Glu Thr Val Leu Arg
1235 1240 1245
Asp Ala Ser Gly Glu Lys Arg Phe Glu Gly Glu Lys Asp Ser Arg
1250 1255 1260
Met Leu Met Asn Val Gly Met Asn Ala Glu Ile Lys Asp Asn Met
1265 1270 1275
Arg Phe Gly Leu Glu Leu Glu Lys Ser Ala Phe Gly Lys Tyr Asn
1280 1285 1290
Val Asp Asn Ala Ile Asn Ala Asn Phe Arg Tyr Ser Phe
1295 1300 1305
<210>37
<211>1068
<212>PRT
<213>大肠杆菌
<400>37
Met Asn Lys Ile Tyr Ala Leu Lys Tyr Cys His Ala Thr Gly Gly Leu
1 5 10 15
Ile Ala Val Ser Glu Leu Ala Ser Arg Val Met Lys Lys Ala Ala Arg
20 25 30
Gly Ser Leu Leu Ala Leu Phe Asn Leu Ser Leu Tyr Gly Ala Phe Leu
35 40 45
Ser Ala Ser Gln Ala Gly Ser Ser Ala Gly Ser Gly Ser Gly Leu Lys
50 55 60
Asn Lys Phe Thr Gln Lys Val Gln Leu Asn Asn Asn Thr Met Ser Leu
65 70 75 80
Asn Ser Asp Ser Phe Thr Leu Ala Gly Asn Asn Thr Ala Val Ala Lys
85 90 95
Asn Asn Asn Asn Tyr Lys Asp Leu Ser Phe Ser Gly Gly Gly Ser Ile
100 105 110
Asn Phe Asp Asn Asp Val Asn Ile Gly Ser Gly Gly Leu Ile Phe Asp
115 120 125
Ala Gly His His Tyr Thr Val Thr Gly Asn Asn Lys Thr Phe Lys Gly
130 135 140
Ala Gly Leu Asp Ile Gly Asp Asn Thr Thr Val Asp Trp Asn Val Lys
145 150 155 160
Gly Val Val Gly Asp Asn Leu His Lys Ile Gly Ala Gly Thr Leu Asn
165 170 175
Val Asn Val Ser Gln Gly Asn Asn Leu Lys Thr Gly Asp Gly Leu Val
180 185 190
Val Leu Asn Ser Ala Asn Ala Phe Asp Asn Ile Tyr Met Ala Ser Gly
195 200 205
His Gly Val Val Lys Ile Asn His Ser Ala Ala Leu Asn Gln Asn Asn
210 215 220
Asp Tyr Arg Gly Ile Phe Phe Thr Glu Asn Gly Gly Thr Leu Asp Leu
225 230 235 240
Asn Gly Tyr Asp Gln Ser Phe Asn Lys Ile Ala Ala Thr Asp Ile Gly
245 250 255
Ala Leu Ile Thr Asn Ser Ala Val Gln Lys Ala Val Leu Ser Val Asn
260 265 270
Asn Gln Ser His Tyr Met Tyr His Gly Ser Val Ser Gly Asn Thr Glu
275 280 285
Ile Asn His Gln Phe Asp Thr Gln Lys Asn Asn Ser Arg Leu Ile Leu
290 295 300
Asp Gly Asn Val Asp Ile Thr Asn Asp Ile Asn Ile Lys Asn Ser Gln
305 310 315 320
Leu His His Ala Gly Thr Cys Leu His Leu Met Leu Phe Leu Glu Arg
325 330 335
Val Gly Val Thr Cys Met Leu Pro Gly Val Ile Cys Glu Lys Asp Tyr
340 345 350
Val Ser Gly Ile Gln Gln Gln Glu Asn Ser Ala Asn Lys Asn Asn Asn
355 360 365
Thr Asp Tyr Lys Thr Asn Asn Gln Val Ser Ser Phe Glu Gln Pro Asp
370 375 380
Trp Glu Asn Arg Leu Phe Lys Phe Lys Thr Leu Asn Leu Ile Asn Ser
385 390 395 400
Asp Phe Ile Val Gly Arg Asn Ala Ile Val Val Gly Asp Ile Ser Ala
405 410 415
Asn Asn Ser Thr Leu Ser Leu Ser Gly Lys Asp Thr Lys Val His Ile
420 425 430
Asp Met Tyr Asp Gly Lys Asn Ile Thr Gly Asp Gly Phe Gly Phe Arg
435 440 445
Gln Asp Ile Lys Asp Gly Val Ser Val Ser Pro Glu Ser Ser Ser Tyr
450 455 460
Phe Gly Asn Val Thr Leu Asn Asn His Ser Leu Leu Asp Ile Gly Asn
465 470 475 480
Lys Phe Thr Gly Gly Ile Glu Ala Tyr Asp Ser Ser Val Ser Val Thr
485 490 495
Ser Gln Asn Ala Val Phe Asp Arg Val Gly Ser Phe Val Asn Ser Ser
500 505 510
Leu Thr Leu Glu Lys Gly Ala Lys Leu Thr Ala Gln Gly Gly Ile Phe
515 520 525
Ser Thr Gly Ala Val Asp Val Lys Glu Asn Ala Ser Leu Ile Leu Thr
530 535 540
Gly Thr Pro Ser Ala Gln Lys Gln Glu Tyr Tyr Ser Pro Val Ile Ser
545 550 555 560
Thr Thr Glu Gly Ile Asn Leu Gly Asp Lys Ala Ser Leu Ser Val Lys
565 570 575
Asn Met Gly Tyr Leu Ser Ser Asp Ile His Ala Gly Thr Thr Ala Ala
580 585 590
Thr Ile Asn Leu Gly Asp Gly Asp Ala Glu Thr Asp Ser Pro Leu Phe
595 600 605
Ser Ser Leu Ile Arg Gly Tyr Asn Ala Val Leu Ser Gly Asn Ile Thr
610 615 620
Gly Glu Gln Ser Thr Val Asn Met Asn Asn Ala Leu Trp Tyr Ser Asp
625 630 635 640
Gly Asn Ser Thr Ile Gly Thr Leu Lys Ser Thr Gly Gly Arg Val Glu
645 650 655
Leu Gly Gly Gly Lys Asp Phe Ala Thr Leu Arg Val Lys Glu Leu Asn
660 665 670
Ala Asn Asn Ala Thr Phe Leu Met His Thr Asn Thr Ser Gln Ala Asp
675 680 685
Gln Leu Asn Val Thr Asn Lys Leu Leu Gly Ser Asn Asn Thr Val Leu
690 695 700
Val Asp Phe Leu Asn Lys Pro Ala Ser Glu Met Asn Val Thr Leu Ile
705 710 715 720
Thr Ala Pro Lys Gly Ser Asp Glu Lys Thr Phe Thr Ala Gly Thr Gln
725 730 735
Gln Ile Gly Phe Ser Asn Val Thr Pro Val Ile Ser Thr Glu Lys Thr
740 745 750
Asp Asp Ala Thr Lys Trp Met Leu Thr Gly Tyr Gln Thr Val Ser Asp
755 760 765
Ala Gly Ala Ser Lys Thr Ala Thr Asp Phe Met Ala Ser Gly Tyr Lys
770 775 780
Ser Phe Leu Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu
785 790 795 800
Arg Asp Thr Gln Gly Asp Ala Gly Val Trp Ala Arg Ile Met Asn Gly
805 810 815
Thr Gly Ser Ala Asp Gly Gly Tyr Ser Asp Asn Tyr Thr His Val Gln
820 825 830
Ile Gly Ala Asp Arg Lys His Glu Leu Asp Gly Val Asp Leu Phe Thr
835 840 845
Gly Ala Leu Leu Thr Tyr Thr Asp Ser Asn Ala Ser Ser His Ala Phe
850 855 860
Ser Gly Lys Thr Lys Ser Val Gly Gly Gly Leu Tyr Ala Ser Ala Leu
865 870 875 880
Phe Asp Ser Gly Ala Tyr Phe Asp Leu Ile Gly Lys Tyr Leu His His
885 890 895
Asp Asn Gln Tyr Thr Ala Ser Phe Ala Ser Leu Gly Thr Lys Asp Tyr
900 905 910
Ser Ser His Ser Trp Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His
915 920 925
Leu Ser Glu Glu Ser Trp Val Glu Pro Gln Met Glu Leu Val Tyr Gly
930 935 940
Ser Val Ser Gly Lys Ser Phe Ile Trp Glu Asp Arg Gly Met Ala Leu
945 950 955 960
Ser Met Lys Asp Lys Asp Tyr Asn Pro Leu Ile Gly Arg Thr Gly Val
965 970 975
Asp Val Gly Arg Thr Phe Ser Gly Asp Asp Trp Lys Ile Thr Ala Arg
980 985 990
Ala Gly Leu Gly Tyr Gln Phe Asp Leu Leu Ala Asn Gly Glu Thr Val
995 1000 1005
Leu Arg Asp Ala Ser Gly Glu Lys Arg Phe Glu Gly Glu Lys Asp
1010 1015 1020
Ser Arg Met Leu Met Asn Val Gly Met Asn Ala Glu Ile Lys Asp
1025 1030 1035
Asn Met Arg Phe Gly Leu Glu Leu Glu Lys Ser Ala Phe Gly Lys
1040 1045 1050
Tyr Asn Val Asp Asn Ala Ile Asn Ala Asn Phe Arg Tyr Ser Phe
1055 1060 1065
<210>38
<211>1162
<212>PRT
<213>大肠杆菌/结核分枝杆菌
<400>38
Met Asn Lys Ile Tyr Ala Leu Lys Tyr Cys His Ala Thr Gly Gly Leu
1 5 10 15
Ile Ala Val Ser Glu Leu Ala Ser Arg Val Met Lys Lys Ala Ala Arg
20 25 30
Gly Ser Leu Leu Ala Leu Phe Asn Leu Ser Leu Tyr Gly Ala Phe Leu
35 40 45
Ser Ala Ser Gln Ala Gly Ser Ser Met Thr Glu Gln Gln Trp Asn Phe
50 55 60
Ala Gly Ile Glu Ala Ala Ala Ser Ala Ile Gln Gly Asn Val Thr Ser
65 70 75 80
Ile His Ser Leu Leu Asp Glu Gly Lys Gln Ser Leu Thr Lys Leu Ala
85 90 95
Ala Ala Trp Gly Gly Ser Gly Ser Glu Ala Tyr Gln Gly Val Gln Gln
100 105 110
Lys Trp Asp Ala Thr Ala Thr Glu Leu Asn Asn Ala Leu Gln Asn Leu
115 120 125
Ala Arg Thr Ile Ser Glu Ala Gly Gln Ala Met Ala Ser Thr Glu Gly
130 135 140
Asn Val Thr Gly Met Phe Ala Gly Ser Gly Ser Gly Leu Lys Asn Lys
145 150 155 160
Phe Thr Gln Lys Val Gln Leu Asn Asn Asn Thr Met Ser Leu Asn Ser
165 170 175
Asp Ser Phe Thr Leu Ala Gly Asn Asn Thr Ala Val Ala Lys Asn Asn
180 185 190
Asn Asn Tyr Lys Asp Leu Ser Phe Ser Gly Gly Gly Ser Ile Asn Phe
195 200 205
Asp Asn Asp Val Asn Ile Gly Ser Gly Gly Leu Ile Phe Asp Ala Gly
210 215 220
His His Tyr Thr Val Thr Gly Asn Asn Lys Thr Phe Lys Gly Ala Gly
225 230 235 240
Leu Asp Ile Gly Asp Asn Thr Thr Val Asp Trp Asn Val Lys Gly Val
245 250 255
Val Gly Asp Asn Leu His Lys Ile Gly Ala Gly Thr Leu Asn Val Asn
260 265 270
Val Ser Gln Gly Asn Asn Leu Lys Thr Gly Asp Gly Leu Val Val Leu
275 280 285
Asn Ser Ala Asn Ala Phe Asp Asn Ile Tyr Met Ala Ser Gly His Gly
290 295 300
Val Val Lys Ile Asn His Ser Ala Ala Leu Asn Gln Asn Asn Asp Tyr
305 310 315 320
Arg Gly Ile Phe Phe Thr Glu Asn Gly Gly Thr Leu Asp Leu Asn Gly
325 330 335
Tyr Asp Gln Ser Phe Asn Lys Ile Ala Ala Thr Asp Ile Gly Ala Leu
340 345 350
Ile Thr Asn Ser Ala Val Gln Lys Ala Val Leu Ser Val Asn Asn Gln
355 360 365
Ser His Tyr Met Tyr His Gly Ser Val Ser Gly Asn Thr Glu Ile Asn
370 375 380
His Gln Phe Asp Thr Gln Lys Asn Asn Ser Arg Leu Ile Leu Asp Gly
385 390 395 400
Asn Val Asp Ile Thr Asn Asp Ile Asn Ile Lys Asn Ser Gln Leu His
405 410 415
His Ala Gly Thr Cys Leu His Leu Met Leu Phe Leu Glu Arg Val Gly
420 425 430
Val Thr Cys Met Leu Pro Gly Val Ile Cys Glu Lys Asp Tyr Val Ser
435 440 445
Gly Ile Gln Gln Gln Glu Asn Ser Ala Asn Lys Asn Asn Asn Thr Asp
450 455 460
Tyr Lys Thr Asn Asn Gln Val Ser Ser Phe Glu Gln Pro Asp Trp Glu
465 470 475 480
Asn Arg Leu Phe Lys Phe Lys Thr Leu Asn Leu Ile Asn Ser Asp Phe
485 490 495
Ile Val Gly Arg Asn Ala Ile Val Val Gly Asp Ile Ser Ala Asn Asn
500 505 510
Ser Thr Leu Ser Leu Ser Gly Lys Asp Thr Lys Val His Ile Asp Met
515 520 525
Tyr Asp Gly Lys Asn Ile Thr Gly Asp Gly Phe Gly Phe Arg Gln Asp
530 535 540
Ile Lys Asp Gly Val Ser Val Ser Pro Glu Ser Ser Ser Tyr Phe Gly
545 550 555 560
Asn Val Thr Leu Asn Asn His Ser Leu Leu Asp Ile Gly Asn Lys Phe
565 570 575
Thr Gly Gly Ile Glu Ala Tyr Asp Ser Ser Val Ser Val Thr Ser Gln
580 585 590
Asn Ala Val Phe Asp Arg Val Gly Ser Phe Val Asn Ser Ser Leu Thr
595 600 605
Leu Glu Lys Gly Ala Lys Leu Thr Ala Gln Gly Gly Ile Phe Ser Thr
610 615 620
Gly Ala Val Asp Val Lys Glu Asn Ala Ser Leu Ile Leu Thr Gly Thr
625 630 635 640
Pro Ser Ala Gln Lys Gln Glu Tyr Tyr Ser Pro Val Ile Ser Thr Thr
645 650 655
Glu Gly Ile Asn Leu Gly Asp Lys Ala Ser Leu Ser Val Lys Asn Met
660 665 670
Gly Tyr Leu Ser Ser Asp Ile His Ala Gly Thr Thr Ala Ala Thr Ile
675 680 685
Asn Leu Gly Asp Gly Asp Ala Glu Thr Asp Ser Pro Leu Phe Ser Ser
690 695 700
Leu Ile Arg Gly Tyr Asn Ala Val Leu Ser Gly Asn Ile Thr Gly Glu
705 710 715 720
Gln Ser Thr Val Asn Met Asn Asn Ala Leu Trp Tyr Ser Asp Gly Asn
725 730 735
Ser Thr Ile Gly Thr Leu Lys Ser Thr Gly Gly Arg Val Glu Leu Gly
740 745 750
Gly Gly Lys Asp Phe Ala Thr Leu Arg Val Lys Glu Leu Asn Ala Asn
755 760 765
Asn Ala Thr Phe Leu Met His Thr Asn Thr Ser Gln Ala Asp Gln Leu
770 775 780
Asn Val Thr Asn Lys Leu Leu Gly Ser Asn Asn Thr Val Leu Val Asp
785 790 795 800
Phe Leu Asn Lys Pro Ala Ser Glu Met Asn Val Thr Leu Ile Thr Ala
805 810 815
Pro Lys Gly Ser Asp Glu Lys Thr Phe Thr Ala Gly Thr Gln Gln Ile
820 825 830
Gly Phe Ser Asn Val Thr Pro Val Ile Ser Thr Glu Lys Thr Asp Asp
835 840 845
Ala Thr Lys Trp Met Leu Thr Gly Tyr Gln Thr Val Ser Asp Ala Gly
850 855 860
Ala Ser Lys Thr Ala Thr Asp Phe Met Ala Ser Gly Tyr Lys Ser Phe
865 870 875 880
Leu Thr Glu Val Asn Asn Leu Asn Lys Arg Met Gly Asp Leu Arg Asp
885 890 895
Thr Gln Gly Asp Ala Gly Val Trp Ala Arg Ile Met Asn Gly Thr Gly
900 905 910
Ser Ala Asp Gly Gly Tyr Ser Asp Asn Tyr Thr His Val Gln Ile Gly
915 920 925
Ala Asp Arg Lys His Glu Leu Asp Gly Val Asp Leu Phe Thr Gly Ala
930 935 940
Leu Leu Thr Tyr Thr Asp Ser Asn Ala Ser Ser His Ala Phe Ser Gly
945 950 955 960
Lys Thr Lys Ser Val Gly Gly Gly Leu Tyr Ala Ser Ala Leu Phe Asp
965 970 975
Ser Gly Ala Tyr Phe Asp Leu Ile Gly Lys Tyr Leu His His Asp Asn
980 985 990
Gln Tyr Thr Ala Ser Phe Ala Ser Leu Gly Thr Lys Asp Tyr Ser Ser
995 1000 1005
His Ser Trp Tyr Ala Gly Ala Glu Val Gly Tyr Arg Tyr His Leu
1010 1015 1020
Ser Glu Glu Ser Trp Val Glu Pro Gln Met Glu Leu Val Tyr Gly
1025 1030 1035
Ser Val Ser Gly Lys Ser Phe Ile Trp Glu Asp Arg Gly Met Ala
1040 1045 1050
Leu Ser Met Lys Asp Lys Asp Tyr Asn Pro Leu Ile Gly Arg Thr
1055 1060 1065
Gly Val Asp Val Gly Arg Thr Phe Ser Gly Asp Asp Trp Lys Ile
1070 1075 1080
Thr Ala Arg Ala Gly Leu Gly Tyr Gln Phe Asp Leu Leu Ala Asn
1085 1090 1095
Gly Glu Thr Val Leu Arg Asp Ala Ser Gly Glu Lys Arg Phe Glu
1100 1105 1110
Gly Glu Lys Asp Ser Arg Met Leu Met Asn Val Gly Met Asn Ala
1115 1120 1125
Glu Ile Lys Asp Asn Met Arg Phe Gly Leu Glu Leu Glu Lys Ser
1130 1135 1140
Ala Phe Gly Lys Tyr Asn Val Asp Asn Ala Ile Asn Ala Asn Phe
1145 1150 1155
Arg Tyr Ser Phe
1160
<210>39
<211>95
<212>PRT
<213>结核分枝杆菌
<400>39
Met Thr Glu Gln Gln Trp Asn Phe Ala Gly Ile Glu Ala Ala Ala Ser
1 5 10 15
Ala Ile Gln Gly Asn Val Thr Ser Ile His Ser Leu Leu Asp Glu Gly
20 25 30
Lys Gln Ser Leu Thr Lys Leu Ala Ala Ala Trp Gly Gly Ser Gly Ser
35 40 45
Glu Ala Tyr Gln Gly Val Gln Gln Lys Trp Asp Ala Thr Ala Thr Glu
50 55 60
Leu Asn Asn Ala Leu Gln Asn Leu Ala Arg Thr Ile Ser Glu Ala Gly
65 70 75 80
Gln Ala Met Ala Ser Thr Glu Gly Asn Val Thr Gly Met Phe Ala
85 90 95
<210>40
<211>285
<212>PRT
<213>结核分枝杆菌
<400>40
Phe Ser Arg Pro Gly Leu Pro Val Glu Tyr Leu Gln Val Pro Ser Pro
1 5 10 15
Ser Met Gly Arg Asp Ile Lys Val Gln Phe Gln Ser Gly Gly Asn Asn
20 25 30
Ser Pro Ala Val Tyr Leu Leu Asp Gly Leu Arg Ala Gln Asp Asp Tyr
35 40 45
Asn Gly Trp Asp Ile Asn Thr Pro Ala Phe Glu Trp Tyr Tyr Gln Ser
50 55 60
Gly Leu Ser Ile Val Met Pro Val Gly Gly Gln Ser Ser Phe Tyr Ser
65 70 75 80
Asp Trp Tyr Ser Pro Ala Cys Gly Lys Ala Gly Cys Gln Thr Tyr Lys
85 90 95
Trp Glu Thr Phe Leu Thr Ser Glu Leu Pro Gln Trp Leu Ser Ala Asn
100 105 110
Arg Ala Val Lys Pro Thr Gly Ser Ala Ala Ile Gly Leu Ser Met Ala
115 120 125
Gly Ser Ser Ala Met Ile Leu Ala Ala Tyr His Pro Gln Gln Phe Ile
130 135 140
Tyr Ala Gly Ser Leu Ser Ala Leu Leu Asp Pro Ser Gln Gly Met Gly
145 150 155 160
Pro Ser Leu Ile Gly Leu Ala Met Gly Asp Ala Gly Gly Tyr Lys Ala
165 170 175
Ala Asn Met Trp Gly Pro Ser Ser Asp Pro Ala Trp Glu Arg Asn Asp
180 185 190
Pro Thr Gln Gln Ile Pro Lys Leu Val Ala Asn Asn Thr Arg Leu Trp
195 200 205
Val Tyr Cys Gly Asn Gly Thr Pro Asn Glu Leu Gly Gly Ala Asn Ile
210 215 220
Pro Ala Glu Phe Leu Lys Asn Phe Val Arg Ser Ser Asn Leu Lys Phe
225 230 235 240
Gln Asp Ala Tyr Asn Ala Ala Gly Gly His Asn Ala Val Phe Asn Phe
245 250 255
Pro Pro Asn Gly Thr His Ser Trp Glu Tyr Trp Gly Ala Gln Leu Asn
260 265 270
Ala Met Lys Gly Asp Leu Gln Ser Ser Leu Gly Ala Gly
275 280 285
<210>41
<211>75
<212>PRT
<213>结核分枝杆菌
<400>41
Val Ile Ala Gly Val Asp Gln Ala Leu Ala Ala Thr Gly Gln Ala Ser
1 5 10 15
Gln Arg Ala Ala Gly Ala Ser Gly Gly Val Thr Val Gly Val Gly Val
20 25 30
Gly Thr Glu Gln Arg Asn Leu Ser Val Val Ala Pro Ser Gln Phe Thr
35 40 45
Phe Ser Ser Arg Ser Pro Asp Phe Val Asp Glu Thr Ala Gly Gln Ser
50 55 60
Trp Cys Ala Ile Leu Gly Leu Asn Gln Phe His
65 70 75
<210>42
<211>96
<212>PRT
<213>结核分枝杆菌
<400>42
Met Ser Gln Ile Met Tyr Asn Tyr Pro Ala Met Leu Gly His Ala Gly
1 5 10 15
Asp Met Ala Gly Tyr Ala Gly Thr Leu Gln Ser Leu Gly Ala Glu Ile
20 25 30
Ala Val Glu Gln Ala Ala Leu Gln Ser Ala Trp Gln Gly Asp Thr Gly
35 40 45
Ile Thr Tyr Gln Ala Trp Gln Ala Gln Trp Asn Gln Ala Met Glu Asp
50 55 60
Leu Val Arg Ala Tyr His Ala Met Ser Ser Thr His Glu Ala Asn Thr
65 70 75 80
Met Ala Met Met Ala Arg Asp Thr Ala Glu Ala Ala Lys Trp Gly Gly
85 90 95
<210>43
<211>96
<212>PRT
<213>结核分枝杆菌
<400>43
Met Ser Gln Ile Met Tyr Asn Tyr Pro Ala Met Met Ala His Ala Gly
1 5 10 15
Asp Met Ala Gly Tyr Ala Gly Thr Leu Gln Ser Leu Gly Ala Asp Ile
20 25 30
Ala Ser Glu Gln Ala Val Leu Ser Ser Ala Trp Gln Gly Asp Thr Gly
35 40 45
Ile Thr Tyr Gln Gly Trp Gln Thr Gln Trp Asn Gln Ala Leu Glu Asp
50 55 60
Leu Val Arg Ala Tyr Gln Ser Met Ser Gly Thr His Glu Ser Asn Thr
65 70 75 80
Met Ala Met Leu Ala Arg Asp Gly Ala Glu Ala Ala Lys Trp Gly Gly
85 90 95
<210>44
<211>11
<212>PRT
<213>人工序列
<220>
<223>人工接头
<400>44
Gly Ser Gly Ser Ser Ala Gly Ser Gly Ser Gly
1 5 10
<210>45
<211>8
<212>PRT
<213>人工序列
<220>
<223>人工接头
<400>45
Ser Ser Cys Gly Ser Gly Ser Gly
1 5
<210>46
<211>9
<212>PRT
<213>人工序列
<220>
<223>人工接头
<400>46
Gly Ser Ser Ala Gly Ser Gly Ser Gly
1 5
<210>47
<211>7
<212>PRT
<213>人工序列
<220>
<223>人工接头
<400>47
Ser Ala Gly Ser Gly Ser Gly
1 5
<210>48
<211>4134
<212>DNA
<213>大肠杆菌
<400>48
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggcat1980
ccggttatcc atgcatacaa tactcagtct gtggctgaca aactggctgc cagtggagac2040
cattcggttc tgactcagcc tacgtcattc agtcaggagg actgggagaa ccgcagtttt2100
acctttgaca ggctgtcact gaagaacact gattttggtc ttggtcgcaa tgccacactg2160
aacacaacca tccaggcaga taactccagc gtcacgctgg gcgacagccg ggtatttatc2220
gacaaaaacg atggccaggg aacagccttt acccttgaag aaggcacatc tgttgcaact2280
aaagatgcag ataaaagtgt cttcaacggc accgtcaacc tggataatca gtcagtgctg2340
aatatcaatg atatattcaa tggcggaata caggcgaaca acagtaccgt gaatatctcc2400
tcagacagtg ccgttctggg gaactcaaca ctgaccagta ccgccctgaa tctgaacaag2460
ggagcaaatg ctctggccag tcagagtttt gtttctgacg gtccagtgaa tatttctgat2520
gccaccctga gtctgaacag ccgtcctgat gaggtatctc acacactttt acctgtatac2580
gattatgccg gttcatggaa cctgaaggga gacgatgccc gcctgaacgt ggggccgtac2640
agtatgttgt caggtaatat caatgttcag gataaaggga ctgtcaccct cggaggggaa2700
ggggaactga gtcctgacct gactcttcag aatcagatgt tgtacagcct gtttaacggg2760
taccgcaata tctggagcgg gagcctgaat gcaccggatg ccaccgtcag catgacagac2820
acccagtggt cgatgaacgg aaactccacg gcaggaaata tgaaacttaa ccggacaata2880
gtcggtttta acgggggaac atcaccgttc acgacactga caacagataa tctggacgcg2940
gttcagtcag catttgtcat gcgtacagac cttaacaagg cagacaaact ggtgataaac3000
aagtcggcaa caggtcatga caacagcatc tgggttaact tcctgaaaaa accttctaac3060
aaggacacgc ttgatattcc actggtcagc gcacctgaag cgacagctga taatctgttc3120
agggcatcaa cacgggttgt gggattcagt gatgtcaccc ccatccttag tgtcagaaaa3180
gaggacggga aaaaagagtg ggtcctcgat ggttaccagg ttgcacgtaa cgacggccag3240
ggtaaggctg ccgccacatt catgcacatc agctataaca acttcatcac tgaagttaac3300
aacctgaaca aacgcatggg cgatttgagg gatattaatg gcgaagccgg tacgtgggtg3360
cgtctgctga acggttccgg ctctgctgat ggcggtttca ctgaccacta taccctgctg3420
cagatggggg ctgaccgtaa gcacgaactg ggaagtatgg acctgtttac cggcgtgatg3480
gccacctaca ctgacacaga tgcgtcagca gacctgtaca gcggtaaaac aaaatcatgg3540
ggtggtggtt tctatgccag tggtctgttc cggtccggcg cttactttga tgtgattgcc3600
aaatatattc acaatgaaaa caaatatgac ctgaactttg ccggagctgg taaacagaac3660
ttccgcagcc attcactgta tgcaggtgca gaagtcggat accgttatca tctgacagat3720
acgacgtttg ttgaacctca ggcggaactg gtctggggaa gactgcaggg ccaaacattt3780
aactggaacg acagtggaat ggatgtctca atgcgtcgta acagcgttaa tcctctggta3840
ggcagaaccg gcgttgtttc cggtaaaacc ttcagtggta aggactggag tctgacagcc3900
cgtgccggcc tgcattatga gttcgatctg acggacagtg ctgacgttca tctgaaggat3960
gcagcgggag aacatcagat taatggcaga aaagacagtc gtatgcttta cggtgtgggg4020
ttaaatgccc ggtttggcga caatacgcgt ctggggctgg aagttgaacg ctctgcattt4080
ggtaaataca acacagatga tgcgataaac gctaatattc gttattcatt ctga 4134
<210>49
<211>4134
<212>DNA
<213>大肠杆菌
<400>49
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggcat1980
ccggttatcc atgcatacaa tactcagtct gtggctgaca aactggctgc cagtggagac2040
cattcggttc tgactcagcc tacgtcattc agtcaggagg actgggagaa ccgcagtttt2100
acctttgaca ggctgtcact gaagaacact gattttggtc ttggtcgcaa tgccacactg2160
aacacaacca tccaggcaga taactccagc gtcacgctgg gcgacagccg ggtatttatc2220
gacaaaaacg atggccaggg aacagccttt acccttgaag aaggcacatc tgttgcaact2280
aaagatgcag ataaaagtgt cttcaacggc accgtcaacc tggataatca gtcagtgctg2340
aatatcaatg atatattcaa tggcggaata caggcgaaca acagtaccgt gaatatctcc2400
tcagacagtg ccgttctggg gaactcaaca ctgaccagta ccgccctgaa tctgaacaag2460
ggagcaaatg ctctggccag tcagagtttt gtttctgacg gtccagtgaa tatttctgat2520
gccaccctga gtctgaacag ccgtcctgat gaggtatctc acacactttt acctgtatac2580
gattatgccg gttcatggaa cctgaaggga gacgatgccc gcctgaacgt ggggccgtac2640
agtatgttgt caggtaatat caatgttcag gataaaggga ctgtcaccct cggaggggaa2700
ggggaactga gtcctgacct gactcttcag aatcagatgt tgtacagcct gtttaacggg2760
taccgcaata tctggagcgg gagcctgaat gcaccggatg ccaccgtcag catgacagac2820
acccagtggt cgatgaacgg aaactccacg gcaggaaata tgaaacttaa ccggacaata2880
gtcggtttta acgggggaac atcaccgttc acgacactga caacagataa tctggacgcg2940
gttcagtcag catttgtcat gcgtacagac cttaacaagg cagacaaact ggtgataaac3000
aagtcggcaa caggtcatga caacagcatc tgggttaact tcctgaaaaa accttctaac3060
aaggacacgc ttgatattcc actggtcagc gcacctgaag cgacagctga taatctgttc3120
agggcatcaa cacgggttgt gggattcagt gatgtcaccc ccatccttag tgtcagaaaa3180
gaggacggga aaaaagagtg ggtcctcgat ggttaccagg ttgcacgtaa cgacggccag3240
ggtaaggctg ccgccacatt catgcacatc agctataaca acttcatcac tgaagttggt3300
tccctgaaca aacgcatggg cgatttgagg gatattaatg gcgaagccgg tacgtgggtg3360
cgtctgctga acggttccgg ctctgctgat ggcggtttca ctgaccacta taccctgctg3420
cagatggggg ctgaccgtaa gcacgaactg ggaagtatgg acctgtttac cggcgtgatg3480
gccacctaca ctgacacaga tgcgtcagca gacctgtaca gcggtaaaac aaaatcatgg3540
ggtggtggtt tctatgccag tggtctgttc cggtccggcg cttactttga tgtgattgcc3600
aaatatattc acaatgaaaa caaatatgac ctgaactttg ccggagctgg taaacagaac3660
ttccgcagcc attcactgta tgcaggtgca gaagtcggat accgttatca tctgacagat3720
acgacgtttg ttgaacctca ggcggaactg gtctggggaa gactgcaggg ccaaacattt3780
aactggaacg acagtggaat ggatgtctca atgcgtcgta acagcgttaa tcctctggta3840
ggcagaaccg gcgttgtttc cggtaaaacc ttcagtggta aggactggag tctgacagcc3900
cgtgccggcc tgcattatga gttcgatctg acggacagtg ctgacgttca tctgaaggat3960
gcagcgggag aacatcagat taatggcaga aaagacagtc gtatgcttta cggtgtgggg4020
ttaaatgccc ggtttggcga caatacgcgt ctggggctgg aagttgaacg ctctgcattt4080
ggtaaataca acacagatga tgcgataaac gctaatattc gttattcatt ctga 4134
<210>50
<211>1338
<212>DNA
<213>大肠杆菌
<400>50
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctcctgcgg atccggcagc180
ggtgcagaca aactggtgat aaacaagtcg gcaacaggtc atgacaacag catctgggtt240
aacttcctga aaaaaccttc taacaaggac acgcttgata ttccactggt cagcgcacct300
gaagcgacag ctgataatct gttcagggca tcaacacggg ttgtgggatt cagtgatgtc360
acccccatcc ttagtgtcag aaaagaggac gggaaaaaag agtgggtcct cgatggttac420
caggttgcac gtaacgacgg ccagggtaag gctgccgcca cattcatgca catcagctat480
aacaacttca tcactgaagt taacaacctg aacaaacgca tgggcgattt gagggatatt540
aatggcgaag ccggtacgtg ggtgcgtctg ctgaacggtt ccggctctgc tgatggcggt600
ttcactgacc actataccct gctgcagatg ggggctgacc gtaagcacga actgggaagt660
atggacctgt ttaccggcgt gatggccacc tacactgaca cagatgcgtc agcagacctg720
tacagcggta aaacaaaatc atggggtggt ggtttctatg ccagtggtct gttccggtcc780
ggcgcttact ttgatgtgat tgccaaatat attcacaatg aaaacaaata tgacctgaac840
tttgccggag ctggtaaaca gaacttccgc agccattcac tgtatgcagg tgcagaagtc900
ggataccgtt atcatctgac agatacgacg tttgttgaac ctcaggcgga actggtctgg960
ggaagactgc agggccaaac atttaactgg aacgacagtg gaatggatgt ctcaatgcgt1020
cgtaacagcg ttaatcctct ggtaggcaga accggcgttg tttccggtaa aaccttcagt1080
ggtaaggact ggagtctgac agcccgtgcc ggcctgcatt atgagttcga tctgacggac1140
agtgctgacg ttcatctgaa ggatgcagcg ggagaacatc agattaatgg cagaaaagac1200
agtcgtatgc tttacggtgt ggggttaaat gcccggtttg gcgacaatac gcgtctgggg1260
ctggaagttg aacgctctgc atttggtaaa tacaacacag atgatgcgat aaacgctaat1320
attcgttatt cattctga 1338
<210>51
<211>3396
<212>DNA
<213>大肠杆菌
<400>51
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctcctgcgg atccggcagc180
ggtaatgatg ccccggtcac gttcagaaca tcggaaggtg gtgcactgga gtggagcttt240
aacagcagta ccggagctgg tgcgctgaca cagggaacca ccacatatgc catgcacggg300
cagcagggaa atgacctgaa tgctggtaag aacctgatat ttcaggggca gaatggtcag360
attaacctta aggattcggt ttctcagggg gcgggttccc tgacgttccg tgataattac420
acagtaacaa cctctaacgg aagtacctgg accggtgccg gtattgttgt ggacaacggg480
gtgtccgtaa actggcaggt taatggtgtt aagggcgata acctgcataa aattggtgaa540
ggtacgctga cggtacaggg tacaggtatt aatgaaggtg gcctgaaggt cggggacgga600
aaggttgtac tgaaccagca ggcggacaat aaaggacagg tgcaggcgtt cagcagtgtt660
aatattgcca gtggccggcc gaccgtggta ctgactgatg agcggcaggt aaatccggat720
accgtctcat ggggatatcg tgggggcaca ctggatgtta atggtaacag tctgacgttt780
catcagttga aggcggcaga ttatggtgcc gtgctggcga ataacgttga taaacgggcc840
actatcacgc tggactatgc cctgcgggct gacaaagtag cactgaatgg ctggtcggaa900
tcaggtaaag gaactgccgg aaatttatat aaatacaata acccgtacac aaatacgacg960
gattacttca tcctgaagca gagcacctat ggttatttcc ccacggacca gagcagcaac1020
gccacctggg agtttgtggg gcacagtcag ggggatgcac agaaactggt agctgaccgt1080
ttcaatactg cagggtatct gtttcacgga caactgaaag gcaatctgaa tgtggacaat1140
cgcctgcctg aaggcgttac cggtgctctg gtgatggacg gagctgcgga tatctccggt1200
acattcaccc aggaaaacgg gcgtctgacg ctgcaggggc atccggttat ccatgcatac1260
aatactcagt ctgtggctga caaactggct gccagtggag accattcggt tctgactcag1320
cctacgtcat tcagtcagga ggactgggag aaccgcagtt ttacctttga caggctgtca1380
ctgaagaaca ctgattttgg tcttggtcgc aatgccacac tgaacacaac catccaggca1440
gataactcca gcgtcacgct gggcgacagc cgggtattta tcgacaaaaa cgatggccag1500
ggaacagcct ttacccttga agaaggcaca tctgttgcaa ctaaagatgc agataaaagt1560
gtcttcaacg gcaccgtcaa cctggataat cagtcagtgc tgaatatcaa tgatatattc1620
aatggcggaa tacaggcgaa caacagtacc gtgaatatct cctcagacag tgccgttctg1680
gggaactcaa cactgaccag taccgccctg aatctgaaca agggagcaaa tgctctggcc1740
agtcagagtt ttgtttctga cggtccagtg aatatttctg atgccaccct gagtctgaac1800
agccgtcctg atgaggtatc tcacacactt ttacctgtat acgattatgc cggttcatgg1860
aacctgaagg gagacgatgc ccgcctgaac gtggggccgt acagtatgtt gtcaggtaat1920
atcaatgttc aggataaagg gactgtcacc ctcggagggg aaggggaact gagtcctgac1980
ctgactcttc agaatcagat gttgtacagc ctgtttaacg ggtaccgcaa tatctggagc2040
gggagcctga atgcaccgga tgccaccgtc agcatgacag acacccagtg gtcgatgaac2100
ggaaactcca cggcaggaaa tatgaaactt aaccggacaa tagtcggttt taacggggga2160
acatcaccgt tcacgacact gacaacagat aatctggacg cggttcagtc agcatttgtc2220
atgcgtacag accttaacaa ggcagacaaa ctggtgataa acaagtcggc aacaggtcat2280
gacaacagca tctgggttaa cttcctgaaa aaaccttcta acaaggacac gcttgatatt2340
ccactggtca gcgcacctga agcgacagct gataatctgt tcagggcatc aacacgggtt2400
gtgggattca gtgatgtcac ccccatcctt agtgtcagaa aagaggacgg gaaaaaagag2460
tgggtcctcg atggttacca ggttgcacgt aacgacggcc agggtaaggc tgccgccaca2520
ttcatgcaca tcagctataa caacttcatc actgaagtta acaacctgaa caaacgcatg2580
ggcgatttga gggatattaa tggcgaagcc ggtacgtggg tgcgtctgct gaacggttcc2640
ggctctgctg atggcggttt cactgaccac tataccctgc tgcagatggg ggctgaccgt2700
aagcacgaac tgggaagtat ggacctgttt accggcgtga tggccaccta cactgacaca2760
gatgcgtcag cagacctgta cagcggtaaa acaaaatcat ggggtggtgg tttctatgcc2820
agtggtctgt tccggtccgg cgcttacttt gatgtgattg ccaaatatat tcacaatgaa2880
aacaaatatg acctgaactt tgccggagct ggtaaacaga acttccgcag ccattcactg2940
tatgcaggtg cagaagtcgg ataccgttat catctgacag atacgacgtt tgttgaacct3000
caggcggaac tggtctgggg aagactgcag ggccaaacat ttaactggaa cgacagtgga3060
atggatgtct caatgcgtcg taacagcgtt aatcctctgg taggcagaac cggcgttgtt3120
tccggtaaaa ccttcagtgg taaggactgg agtctgacag cccgtgccgg cctgcattat3180
gagttcgatc tgacggacag tgctgacgtt catctgaagg atgcagcggg agaacatcag3240
attaatggca gaaaagacag tcgtatgctt tacggtgtgg ggttaaatgc ccggtttggc3300
gacaatacgc gtctggggct ggaagttgaa cgctctgcat ttggtaaata caacacagat3360
gatgcgataa acgctaatat tcgttattca ttctga 3396
<210>52
<211>3396
<212>DNA
<213>大肠杆菌
<400>52
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctcctgcgg atccggcagc180
ggtaatgatg ccccggtcac gttcagaaca tcggaaggtg gtgcactgga gtggagcttt240
aacagcagta ccggagctgg tgcgctgaca cagggaacca ccacatatgc catgcacggg300
cagcagggaa atgacctgaa tgctggtaag aacctgatat ttcaggggca gaatggtcag360
attaacctta aggattcggt ttctcagggg gcgggttccc tgacgttccg tgataattac420
acagtaacaa cctctaacgg aagtacctgg accggtgccg gtattgttgt ggacaacggg480
gtgtccgtaa actggcaggt taatggtgtt aagggcgata acctgcataa aattggtgaa540
ggtacgctga cggtacaggg tacaggtatt aatgaaggtg gcctgaaggt cggggacgga600
aaggttgtac tgaaccagca ggcggacaat aaaggacagg tgcaggcgtt cagcagtgtt660
aatattgcca gtggccggcc gaccgtggta ctgactgatg agcggcaggt aaatccggat720
accgtctcat ggggatatcg tgggggcaca ctggatgtta atggtaacag tctgacgttt780
catcagttga aggcggcaga ttatggtgcc gtgctggcga ataacgttga taaacgggcc840
actatcacgc tggactatgc cctgcgggct gacaaagtag cactgaatgg ctggtcggaa900
tcaggtaaag gaactgccgg aaatttatat aaatacaata acccgtacac aaatacgacg960
gattacttca tcctgaagca gagcacctat ggttatttcc ccacggacca gagcagcaac1020
gccacctggg agtttgtggg gcacagtcag ggggatgcac agaaactggt agctgaccgt1080
ttcaatactg cagggtatct gtttcacgga caactgaaag gcaatctgaa tgtggacaat1140
cgcctgcctg aaggcgttac cggtgctctg gtgatggacg gagctgcgga tatctccggt1200
acattcaccc aggaaaacgg gcgtctgacg ctgcaggggc atccggttat ccatgcatac1260
aatactcagt ctgtggctga caaactggct gccagtggag accattcggt tctgactcag1320
cctacgtcat tcagtcagga ggactgggag aaccgcagtt ttacctttga caggctgtca1380
ctgaagaaca ctgattttgg tcttggtcgc aatgccacac tgaacacaac catccaggca1440
gataactcca gcgtcacgct gggcgacagc cgggtattta tcgacaaaaa cgatggccag1500
ggaacagcct ttacccttga agaaggcaca tctgttgcaa ctaaagatgc agataaaagt1560
gtcttcaacg gcaccgtcaa cctggataat cagtcagtgc tgaatatcaa tgatatattc1620
aatggcggaa tacaggcgaa caacagtacc gtgaatatct cctcagacag tgccgttctg1680
gggaactcaa cactgaccag taccgccctg aatctgaaca agggagcaaa tgctctggcc1740
agtcagagtt ttgtttctga cggtccagtg aatatttctg atgccaccct gagtctgaac1800
agccgtcctg atgaggtatc tcacacactt ttacctgtat acgattatgc cggttcatgg1860
aacctgaagg gagacgatgc ccgcctgaac gtggggccgt acagtatgtt gtcaggtaat1920
atcaatgttc aggataaagg gactgtcacc ctcggagggg aaggggaact gagtcctgac1980
ctgactcttc agaatcagat gttgtacagc ctgtttaacg ggtaccgcaa tatctggagc2040
gggagcctga atgcaccgga tgccaccgtc agcatgacag acacccagtg gtcgatgaac2100
ggaaactcca cggcaggaaa tatgaaactt aaccggacaa tagtcggttt taacggggga2160
acatcaccgt tcacgacact gacaacagat aatctggacg cggttcagtc agcatttgtc2220
atgcgtacag accttaacaa ggcagacaaa ctggtgataa acaagtcggc aacaggtcat2280
gacaacagca tctgggttaa cttcctgaaa aaaccttcta acaaggacac gcttgatatt2340
ccactggtca gcgcacctga agcgacagct gataatctgt tcagggcatc aacacgggtt2400
gtgggattca gtgatgtcac ccccatcctt agtgtcagaa aagaggacgg gaaaaaagag2460
tgggtcctcg atggttacca ggttgcacgt aacgacggcc agggtaaggc tgccgccaca2520
ttcatgcaca tcagctataa caacttcatc actgaagttg gttccctgaa caaacgcatg2580
ggcgatttga gggatattaa tggcgaagcc ggtacgtggg tgcgtctgct gaacggttcc2640
ggctctgctg atggcggttt cactgaccac tataccctgc tgcagatggg ggctgaccgt2700
aagcacgaac tgggaagtat ggacctgttt accggcgtga tggccaccta cactgacaca2760
gatgcgtcag cagacctgta cagcggtaaa acaaaatcat ggggtggtgg tttctatgcc2820
agtggtctgt tccggtccgg cgcttacttt gatgtgattg ccaaatatat tcacaatgaa2880
aacaaatatg acctgaactt tgccggagct ggtaaacaga acttccgcag ccattcactg2940
tatgcaggtg cagaagtcgg ataccgttat catctgacag atacgacgtt tgttgaacct3000
caggcggaac tggtctgggg aagactgcag ggccaaacat ttaactggaa cgacagtgga3060
atggatgtct caatgcgtcg taacagcgtt aatcctctgg taggcagaac cggcgttgtt3120
tccggtaaaa ccttcagtgg taaggactgg agtctgacag cccgtgccgg cctgcattat3180
gagttcgatc tgacggacag tgctgacgtt catctgaagg atgcagcggg agaacatcag3240
attaatggca gaaaagacag tcgtatgctt tacggtgtgg ggttaaatgc ccggtttggc3300
gacaatacgc gtctggggct ggaagttgaa cgctctgcat ttggtaaata caacacagat3360
gatgcgataa acgctaatat tcgttattca ttctga 3396
<210>53
<211>3945
<212>DNA
<213>大肠杆菌
<400>53
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccg gttctgggag ctccgcagga1620
tccggcagcg gtaatactgc agggtatctg tttcacggac aactgaaagg caatctgaat1680
gtggacaatc gcctgcctga aggcgttacc ggtgctctgg tgatggacgg agctgcggat1740
atctccggta cattcaccca ggaaaacggg cgtctgacgc tgcaggggca tccggttatc1800
catgcataca atactcagtc tgtggctgac aaactggctg ccagtggaga ccattcggtt1860
ctgactcagc ctacgtcatt cagtcaggag gactgggaga accgcagttt tacctttgac1920
aggctgtcac tgaagaacac tgattttggt cttggtcgca atgccacact gaacacaacc1980
atccaggcag ataactccag cgtcacgctg ggcgacagcc gggtatttat cgacaaaaac2040
gatggccagg gaacagcctt tacccttgaa gaaggcacat ctgttgcaac taaagatgca2100
gataaaagtg tcttcaacgg caccgtcaac ctggataatc agtcagtgct gaatatcaat2160
gatatattca atggcggaat acaggcgaac aacagtaccg tgaatatctc ctcagacagt2220
gccgttctgg ggaactcaac actgaccagt accgccctga atctgaacaa gggagcaaat2280
gctctggcca gtcagagttt tgtttctgac ggtccagtga atatttctga tgccaccctg2340
agtctgaaca gccgtcctga tgaggtatct cacacacttt tacctgtata cgattatgcc2400
ggttcatgga acctgaaggg agacgatgcc cgcctgaacg tggggccgta cagtatgttg2460
tcaggtaata tcaatgttca ggataaaggg actgtcaccc tcggagggga aggggaactg2520
agtcctgacc tgactcttca gaatcagatg ttgtacagcc tgtttaacgg gtaccgcaat2580
atctggagcg ggagcctgaa tgcaccggat gccaccgtca gcatgacaga cacccagtgg2640
tcgatgaacg gaaactccac ggcaggaaat atgaaactta accggacaat agtcggtttt2700
aacgggggaa catcaccgtt cacgacactg acaacagata atctggacgc ggttcagtca2760
gcatttgtca tgcgtacaga ccttaacaag gcagacaaac tggtgataaa caagtcggca2820
acaggtcatg acaacagcat ctgggttaac ttcctgaaaa aaccttctaa caaggacacg2880
cttgatattc cactggtcag cgcacctgaa gcgacagctg ataatctgtt cagggcatca2940
acacgggttg tgggattcag tgatgtcacc cccatcctta gtgtcagaaa agaggacggg3000
aaaaaagagt gggtcctcga tggttaccag gttgcacgta acgacggcca gggtaaggct3060
gccgccacat tcatgcacat cagctataac aacttcatca ctgaagttaa caacctgaac3120
aaacgcatgg gcgatttgag ggatattaat ggcgaagccg gtacgtgggt gcgtctgctg3180
aacggttccg gctctgctga tggcggtttc actgaccact ataccctgct gcagatgggg3240
gctgaccgta agcacgaact gggaagtatg gacctgttta ccggcgtgat ggccacctac3300
actgacacag atgcgtcagc agacctgtac agcggtaaaa caaaatcatg gggtggtggt3360
ttctatgcca gtggtctgtt ccggtccggc gcttactttg atgtgattgc caaatatatt3420
cacaatgaaa acaaatatga cctgaacttt gccggagctg gtaaacagaa cttccgcagc3480
cattcactgt atgcaggtgc agaagtcgga taccgttatc atctgacaga tacgacgttt3540
gttgaacctc aggcggaact ggtctgggga agactgcagg gccaaacatt taactggaac3600
gacagtggaa tggatgtctc aatgcgtcgt aacagcgtta atcctctggt aggcagaacc3660
ggcgttgttt ccggtaaaac cttcagtggt aaggactgga gtctgacagc ccgtgccggc3720
ctgcattatg agttcgatct gacggacagt gctgacgttc atctgaagga tgcagcggga3780
gaacatcaga ttaatggcag aaaagacagt cgtatgcttt acggtgtggg gttaaatgcc3840
cggtttggcg acaatacgcg tctggggctg gaagttgaac gctctgcatt tggtaaatac3900
aacacagatg atgcgataaa cgctaatatt cgttattcat tctga 3945
<210>54
<211>3945
<212>DNA
<213>大肠杆菌
<400>54
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccg gttctgggag ctccgcagga1620
tccggcagcg gtaatactgc agggtatctg tttcacggac aactgaaagg caatctgaat1680
gtggacaatc gcctgcctga aggcgttacc ggtgctctgg tgatggacgg agctgcggat1740
atctccggta cattcaccca ggaaaacggg cgtctgacgc tgcaggggca tccggttatc1800
catgcataca atactcagtc tgtggctgac aaactggctg ccagtggaga ccattcggtt1860
ctgactcagc ctacgtcatt cagtcaggag gactgggaga accgcagttt tacctttgac1920
aggctgtcac tgaagaacac tgattttggt cttggtcgca atgccacact gaacacaacc1980
atccaggcag ataactccag cgtcacgctg ggcgacagcc gggtatttat cgacaaaaac2040
gatggccagg gaacagcctt tacccttgaa gaaggcacat ctgttgcaac taaagatgca2100
gataaaagtg tcttcaacgg caccgtcaac ctggataatc agtcagtgct gaatatcaat2160
gatatattca atggcggaat acaggcgaac aacagtaccg tgaatatctc ctcagacagt2220
gccgttctgg ggaactcaac actgaccagt accgccctga atctgaacaa gggagcaaat2280
gctctggcca gtcagagttt tgtttctgac ggtccagtga atatttctga tgccaccctg2340
agtctgaaca gccgtcctga tgaggtatct cacacacttt tacctgtata cgattatgcc2400
ggttcatgga acctgaaggg agacgatgcc cgcctgaacg tggggccgta cagtatgttg2460
tcaggtaata tcaatgttca ggataaaggg actgtcaccc tcggagggga aggggaactg2520
agtcctgacc tgactcttca gaatcagatg ttgtacagcc tgtttaacgg gtaccgcaat2580
atctggagcg ggagcctgaa tgcaccggat gccaccgtca gcatgacaga cacccagtgg2640
tcgatgaacg gaaactccac ggcaggaaat atgaaactta accggacaat agtcggtttt2700
aacgggggaa catcaccgtt cacgacactg acaacagata atctggacgc ggttcagtca2760
gcatttgtca tgcgtacaga ccttaacaag gcagacaaac tggtgataaa caagtcggca2820
acaggtcatg acaacagcat ctgggttaac ttcctgaaaa aaccttctaa caaggacacg2880
cttgatattc cactggtcag cgcacctgaa gcgacagctg ataatctgtt cagggcatca2940
acacgggttg tgggattcag tgatgtcacc cccatcctta gtgtcagaaa agaggacggg3000
aaaaaagagt gggtcctcga tggttaccag gttgcacgta acgacggcca gggtaaggct3060
gccgccacat tcatgcacat cagctataac aacttcatca ctgaagttgg ttccctgaac3120
aaacgcatgg gcgatttgag ggatattaat ggcgaagccg gtacgtgggt gcgtctgctg3180
aacggttccg gctctgctga tggcggtttc actgaccact ataccctgct gcagatgggg3240
gctgaccgta agcacgaact gggaagtatg gacctgttta ccggcgtgat ggccacctac3300
actgacacag atgcgtcagc agacctgtac agcggtaaaa caaaatcatg gggtggtggt3360
ttctatgcca gtggtctgtt ccggtccggc gcttactttg atgtgattgc caaatatatt3420
cacaatgaaa acaaatatga cctgaacttt gccggagctg gtaaacagaa cttccgcagc3480
cattcactgt atgcaggtgc agaagtcgga taccgttatc atctgacaga tacgacgttt3540
gttgaacctc aggcggaact ggtctgggga agactgcagg gccaaacatt taactggaac3600
gacagtggaa tggatgtctc aatgcgtcgt aacagcgtta atcctctggt aggcagaacc3660
ggcgttgttt ccggtaaaac cttcagtggt aaggactgga gtctgacagc ccgtgccggc3720
ctgcattatg agttcgatct gacggacagt gctgacgttc atctgaagga tgcagcggga3780
gaacatcaga ttaatggcag aaaagacagt cgtatgcttt acggtgtggg gttaaatgcc3840
cggtttggcg acaatacgcg tctggggctg gaagttgaac gctctgcatt tggtaaatac3900
aacacagatg atgcgataaa cgctaatatt cgttattcat tctga 3945
<210>55
<211>4053
<212>DNA
<213>大肠杆菌
<400>55
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggagc1980
gggagctccg caggatccgg cagcggtaac cgcagtttta cctttgacag gctgtcactg2040
aagaacactg attttggtct tggtcgcaat gccacactga acacaaccat ccaggcagat2100
aactccagcg tcacgctggg cgacagccgg gtatttatcg acaaaaacga tggccaggga2160
acagccttta cccttgaaga aggcacatct gttgcaacta aagatgcaga taaaagtgtc2220
ttcaacggca ccgtcaacct ggataatcag tcagtgctga atatcaatga tatattcaat2280
ggcggaatac aggcgaacaa cagtaccgtg aatatctcct cagacagtgc cgttctgggg2340
aactcaacac tgaccagtac cgccctgaat ctgaacaagg gagcaaatgc tctggccagt2400
cagagttttg tttctgacgg tccagtgaat atttctgatg ccaccctgag tctgaacagc2460
cgtcctgatg aggtatctca cacactttta cctgtatacg attatgccgg ttcatggaac2520
ctgaagggag acgatgcccg cctgaacgtg gggccgtaca gtatgttgtc aggtaatatc2580
aatgttcagg ataaagggac tgtcaccctc ggaggggaag gggaactgag tcctgacctg2640
actcttcaga atcagatgtt gtacagcctg tttaacgggt accgcaatat ctggagcggg2700
agcctgaatg caccggatgc caccgtcagc atgacagaca cccagtggtc gatgaacgga2760
aactccacgg caggaaatat gaaacttaac cggacaatag tcggttttaa cgggggaaca2820
tcaccgttca cgacactgac aacagataat ctggacgcgg ttcagtcagc atttgtcatg2880
cgtacagacc ttaacaaggc agacaaactg gtgataaaca agtcggcaac aggtcatgac2940
aacagcatct gggttaactt cctgaaaaaa ccttctaaca aggacacgct tgatattcca3000
ctggtcagcg cacctgaagc gacagctgat aatctgttca gggcatcaac acgggttgtg3060
ggattcagtg atgtcacccc catccttagt gtcagaaaag aggacgggaa aaaagagtgg3120
gtcctcgatg gttaccaggt tgcacgtaac gacggccagg gtaaggctgc cgccacattc3180
atgcacatca gctataacaa cttcatcact gaagttaaca acctgaacaa acgcatgggc3240
gatttgaggg atattaatgg cgaagccggt acgtgggtgc gtctgctgaa cggttccggc3300
tctgctgatg gcggtttcac tgaccactat accctgctgc agatgggggc tgaccgtaag3360
cacgaactgg gaagtatgga cctgtttacc ggcgtgatgg ccacctacac tgacacagat3420
gcgtcagcag acctgtacag cggtaaaaca aaatcatggg gtggtggttt ctatgccagt3480
ggtctgttcc ggtccggcgc ttactttgat gtgattgcca aatatattca caatgaaaac3540
aaatatgacc tgaactttgc cggagctggt aaacagaact tccgcagcca ttcactgtat3600
gcaggtgcag aagtcggata ccgttatcat ctgacagata cgacgtttgt tgaacctcag3660
gcggaactgg tctggggaag actgcagggc caaacattta actggaacga cagtggaatg3720
gatgtctcaa tgcgtcgtaa cagcgttaat cctctggtag gcagaaccgg cgttgtttcc3780
ggtaaaacct tcagtggtaa ggactggagt ctgacagccc gtgccggcct gcattatgag3840
ttcgatctga cggacagtgc tgacgttcat ctgaaggatg cagcgggaga acatcagatt3900
aatggcagaa aagacagtcg tatgctttac ggtgtggggt taaatgcccg gtttggcgac3960
aatacgcgtc tggggctgga agttgaacgc tctgcatttg gtaaatacaa cacagatgat4020
gcgataaacg ctaatattcg ttattcattc tga 4053
<210>56
<211>4077
<212>DNA
<213>大肠杆菌
<400>56
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggcat1980
ccggttatcc atgcatacaa tactcagtct gtggctgaca aactggctgc cagtggagac2040
cattcggttc tgactcagcc tacgtcattc agtcaggagg actgggagaa ccgcagtttt2100
acctttgaca ggctgtcact gaagaacact gattttggtc ttggtcgcaa tgccacactg2160
aacacaacca tccaggcaga taactccagc gtcacgctgg gcgacgggag cgggagctcc2220
gcaggatccg gcagcggtag tgtcttcaac ggcaccgtca acctggataa tcagtcagtg2280
ctgaatatca atgatatatt caatggcgga atacaggcga acaacagtac cgtgaatatc2340
tcctcagaca gtgccgttct ggggaactca acactgacca gtaccgccct gaatctgaac2400
aagggagcaa atgctctggc cagtcagagt tttgtttctg acggtccagt gaatatttct2460
gatgccaccc tgagtctgaa cagccgtcct gatgaggtat ctcacacact tttacctgta2520
tacgattatg ccggttcatg gaacctgaag ggagacgatg cccgcctgaa cgtggggccg2580
tacagtatgt tgtcaggtaa tatcaatgtt caggataaag ggactgtcac cctcggaggg2640
gaaggggaac tgagtcctga cctgactctt cagaatcaga tgttgtacag cctgtttaac2700
gggtaccgca atatctggag cgggagcctg aatgcaccgg atgccaccgt cagcatgaca2760
gacacccagt ggtcgatgaa cggaaactcc acggcaggaa atatgaaact taaccggaca2820
atagtcggtt ttaacggggg aacatcaccg ttcacgacac tgacaacaga taatctggac2880
gcggttcagt cagcatttgt catgcgtaca gaccttaaca aggcagacaa actggtgata2940
aacaagtcgg caacaggtca tgacaacagc atctgggtta acttcctgaa aaaaccttct3000
aacaaggaca cgcttgatat tccactggtc agcgcacctg aagcgacagc tgataatctg3060
ttcagggcat caacacgggt tgtgggattc agtgatgtca cccccatcct tagtgtcaga3120
aaagaggacg ggaaaaaaga gtgggtcctc gatggttacc aggttgcacg taacgacggc3180
cagggtaagg ctgccgccac attcatgcac atcagctata acaacttcat cactgaagtt3240
aacaacctga acaaacgcat gggcgatttg agggatatta atggcgaagc cggtacgtgg3300
gtgcgtctgc tgaacggttc cggctctgct gatggcggtt tcactgacca ctataccctg3360
ctgcagatgg gggctgaccg taagcacgaa ctgggaagta tggacctgtt taccggcgtg3420
atggccacct acactgacac agatgcgtca gcagacctgt acagcggtaa aacaaaatca3480
tggggtggtg gtttctatgc cagtggtctg ttccggtccg gcgcttactt tgatgtgatt3540
gccaaatata ttcacaatga aaacaaatat gacctgaact ttgccggagc tggtaaacag3600
aacttccgca gccattcact gtatgcaggt gcagaagtcg gataccgtta tcatctgaca3660
gatacgacgt ttgttgaacc tcaggcggaa ctggtctggg gaagactgca gggccaaaca3720
tttaactgga acgacagtgg aatggatgtc tcaatgcgtc gtaacagcgt taatcctctg3780
gtaggcagaa ccggcgttgt ttccggtaaa accttcagtg gtaaggactg gagtctgaca3840
gcccgtgccg gcctgcatta tgagttcgat ctgacggaca gtgctgacgt tcatctgaag3900
gatgcagcgg gagaacatca gattaatggc agaaaagaca gtcgtatgct ttacggtgtg3960
gggttaaatg cccggtttgg cgacaatacg cgtctggggc tggaagttga acgctctgca4020
tttggtaaat acaacacaga tgatgcgata aacgctaata ttcgttattc attctga 4077
<210>57
<211>4101
<212>DNA
<213>大肠杆菌
<400>57
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggcat1980
ccggttatcc atgcatacaa tactcagtct gtggctgaca aactggctgc cagtggagac2040
cattcggttc tgactcagcc tacgtcattc agtcaggagg actgggagaa ccgcagtttt2100
acctttgaca ggctgtcact gaagaacact gattttggtc ttggtcgcaa tgccacactg2160
aacacaacca tccaggcaga taactccagc gtcacgctgg gcgacagccg ggtatttatc2220
gacaaaaacg atggccaggg aacagccttt acccttgaag aaggcacatc tgttgcaact2280
aaagatgcag ataaaagtgt cttcaacggc accgtcaacc tggataatca gtcagtgctg2340
aatatcaatg atatattcaa tggcggaata caggcgaaca acagtaccgt gaatatctcc2400
tcagacagtg ccgttctggg gaactcaaca ctgaccagta ccgccctgaa tctgaacaag2460
ggagcaaatg ctctggccag tcagagtttt gtttctgacg gtccagtgaa tatttctgat2520
gccaccctga gtctgaacag ccgtcctgat gaggtatctc acacactttt acctgtatac2580
gattatgccg gttcatggaa cctgaaggga gacgatgccc gcctgaacgt ggggccgtac2640
agtatgttgt caggtaatat caatgttcag gataaaggga ctgtcaccct cggagggagc2700
gggagctccg caggatccgg cagcgggtac cgcaatatct ggagcgggag cctgaatgca2760
ccggatgcca ccgtcagcat gacagacacc cagtggtcga tgaacggaaa ctccacggca2820
ggaaatatga aacttaaccg gacaatagtc ggttttaacg ggggaacatc accgttcacg2880
acactgacaa cagataatct ggacgcggtt cagtcagcat ttgtcatgcg tacagacctt2940
aacaaggcag acaaactggt gataaacaag tcggcaacag gtcatgacaa cagcatctgg3000
gttaacttcc tgaaaaaacc ttctaacaag gacacgcttg atattccact ggtcagcgca3060
cctgaagcga cagctgataa tctgttcagg gcatcaacac gggttgtggg attcagtgat3120
gtcaccccca tccttagtgt cagaaaagag gacgggaaaa aagagtgggt cctcgatggt3180
taccaggttg cacgtaacga cggccagggt aaggctgccg ccacattcat gcacatcagc3240
tataacaact tcatcactga agttaacaac ctgaacaaac gcatgggcga tttgagggat3300
attaatggcg aagccggtac gtgggtgcgt ctgctgaacg gttccggctc tgctgatggc3360
ggtttcactg accactatac cctgctgcag atgggggctg accgtaagca cgaactggga3420
agtatggacc tgtttaccgg cgtgatggcc acctacactg acacagatgc gtcagcagac3480
ctgtacagcg gtaaaacaaa atcatggggt ggtggtttct atgccagtgg tctgttccgg3540
tccggcgctt actttgatgt gattgccaaa tatattcaca atgaaaacaa atatgacctg3600
aactttgccg gagctggtaa acagaacttc cgcagccatt cactgtatgc aggtgcagaa3660
gtcggatacc gttatcatct gacagatacg acgtttgttg aacctcaggc ggaactggtc3720
tggggaagac tgcagggcca aacatttaac tggaacgaca gtggaatgga tgtctcaatg3780
cgtcgtaaca gcgttaatcc tctggtaggc agaaccggcg ttgtttccgg taaaaccttc3840
agtggtaagg actggagtct gacagcccgt gccggcctgc attatgagtt cgatctgacg3900
gacagtgctg acgttcatct gaaggatgca gcgggagaac atcagattaa tggcagaaaa3960
gacagtcgta tgctttacgg tgtggggtta aatgcccggt ttggcgacaa tacgcgtctg4020
gggctggaag ttgaacgctc tgcatttggt aaatacaaca cagatgatgc gataaacgct4080
aatattcgtt attcattctg a 4101
<210>58
<211>4152
<212>DNA
<213>大肠杆菌
<400>58
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggcat1980
ccggttatcc atgcatacaa tactcagtct gtggctgaca aactggctgc cagtggagac2040
cattcggttc tgactcagcc tacgtcattc agtcaggagg actgggagaa ccgcagtttt2100
acctttgaca ggctgtcact gaagaacact gattttggtc ttggtcgcaa tgccacactg2160
aacacaacca tccaggcaga taactccagc gtcacgctgg gcgacagccg ggtatttatc2220
gacaaaaacg atggccaggg aacagccttt acccttgaag aaggcacatc tgttgcaggt2280
tctgggagct ccgcaggatc cggcagcggt aaaagtgtct tcaacggcac cgtcaacctg2340
gataatcagt cagtgctgaa tatcaatgat atattcaatg gcggaataca ggcgaacaac2400
agtaccgtga atatctcctc agacagtgcc gttctgggga actcaacact gaccagtacc2460
gccctgaatc tgaacaaggg agcaaatgct ctggccagtc agagttttgt ttctgacggt2520
ccagtgaata tttctgatgc caccctgagt ctgaacagcc gtcctgatga ggtatctcac2580
acacttttac ctgtatacga ttatgccggt tcatggaacc tgaagggaga cgatgcccgc2640
ctgaacgtgg ggccgtacag tatgttgtca ggtaatatca atgttcagga taaagggact2700
gtcaccctcg gaggggaagg ggaactgagt cctgacctga ctcttcagaa tcagatgttg2760
tacagcctgt ttaacgggta ccgcaatatc tggagcggga gcctgaatgc accggatgcc2820
accgtcagca tgacagacac ccagtggtcg atgaacggaa actccacggc aggaaatatg2880
aaacttaacc ggacaatagt cggttttaac gggggaacat caccgttcac gacactgaca2940
acagataatc tggacgcggt tcagtcagca tttgtcatgc gtacagacct taacaaggca3000
gacaaactgg tgataaacaa gtcggcaaca ggtcatgaca acagcatctg ggttaacttc3060
ctgaaaaaac cttctaacaa ggacacgctt gatattccac tggtcagcgc acctgaagcg3120
acagctgata atctgttcag ggcatcaaca cgggttgtgg gattcagtga tgtcaccccc3180
atccttagtg tcagaaaaga ggacgggaaa aaagagtggg tcctcgatgg ttaccaggtt3240
gcacgtaacg acggccaggg taaggctgcc gccacattca tgcacatcag ctataacaac3300
ttcatcactg aagttaacaa cctgaacaaa cgcatgggcg atttgaggga tattaatggc3360
gaagccggta cgtgggtgcg tctgctgaac ggttccggct ctgctgatgg cggtttcact3420
gaccactata ccctgctgca gatgggggct gaccgtaagc acgaactggg aagtatggac3480
ctgtttaccg gcgtgatggc cacctacact gacacagatg cgtcagcaga cctgtacagc3540
ggtaaaacaa aatcatgggg tggtggtttc tatgccagtg gtctgttccg gtccggcgct3600
tactttgatg tgattgccaa atatattcac aatgaaaaca aatatgacct gaactttgcc3660
ggagctggta aacagaactt ccgcagccat tcactgtatg caggtgcaga agtcggatac3720
cgttatcatc tgacagatac gacgtttgtt gaacctcagg cggaactggt ctggggaaga3780
ctgcagggcc aaacatttaa ctggaacgac agtggaatgg atgtctcaat gcgtcgtaac3840
agcgttaatc ctctggtagg cagaaccggc gttgtttccg gtaaaacctt cagtggtaag3900
gactggagtc tgacagcccg tgccggcctg cattatgagt tcgatctgac ggacagtgct3960
gacgttcatc tgaaggatgc agcgggagaa catcagatta atggcagaaa agacagtcgt4020
atgctttacg gtgtggggtt aaatgcccgg tttggcgaca atacgcgtct ggggctggaa4080
gttgaacgct ctgcatttgg taaatacaac acagatgatg cgataaacgc taatattcgt4140
tattcattct ga 4152
<210>59
<211>1620
<212>DNA
<213> 大肠杆菌/结核分枝杆菌
<400>59
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccatgac cgaacagcag180
tggaacttcg cgggtatcga agcggcggcg agcgcgatcc agggtaacgt gaccagcatc240
cacagcctgc tggatgaagg taaacagagc ctgaccaaac tggcggcggc ctggggtggt300
agcggtagcg aagcgtatca gggtgtgcag cagaaatggg atgcgaccgc gaccgaactg360
aacaacgcgc tgcagaacct ggcgcgtacc atcagcgaag cgggtcaggc gatggcgagc420
accgaaggta acgtgaccgg tatgttcgcg ggatccggca gcggtgcaga caaactggtg480
ataaacaagt cggcaacagg tcatgacaac agcatctggg ttaacttcct gaaaaaacct540
tctaacaagg acacgcttga tattccactg gtcagcgcac ctgaagcgac agctgataat600
ctgttcaggg catcaacacg ggttgtggga ttcagtgatg tcacccccat ccttagtgtc660
agaaaagagg acgggaaaaa agagtgggtc ctcgatggtt accaggttgc acgtaacgac720
ggccagggta aggctgccgc cacattcatg cacatcagct ataacaactt catcactgaa780
gttaacaacc tgaacaaacg catgggcgat ttgagggata ttaatggcga agccggtacg840
tgggtgcgtc tgctgaacgg ttccggctct gctgatggcg gtttcactga ccactatacc900
ctgctgcaga tgggggctga ccgtaagcac gaactgggaa gtatggacct gtttaccggc960
gtgatggcca cctacactga cacagatgcg tcagcagacc tgtacagcgg taaaacaaaa1020
tcatggggtg gtggtttcta tgccagtggt ctgttccggt ccggcgctta ctttgatgtg1080
attgccaaat atattcacaa tgaaaacaaa tatgacctga actttgccgg agctggtaaa1140
cagaacttcc gcagccattc actgtatgca ggtgcagaag tcggataccg ttatcatctg1200
acagatacga cgtttgttga acctcaggcg gaactggtct ggggaagact gcagggccaa1260
acatttaact ggaacgacag tggaatggat gtctcaatgc gtcgtaacag cgttaatcct1320
ctggtaggca gaaccggcgt tgtttccggt aaaaccttca gtggtaagga ctggagtctg1380
acagcccgtg ccggcctgca ttatgagttc gatctgacgg acagtgctga cgttcatctg1440
aaggatgcag cgggagaaca tcagattaat ggcagaaaag acagtcgtat gctttacggt1500
gtggggttaa atgcccggtt tggcgacaat acgcgtctgg ggctggaagt tgaacgctct1560
gcatttggta aatacaacac agatgatgcg ataaacgcta atattcgtta ttcattctga1620
<210>60
<211>3678
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>60
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccatgac cgaacagcag180
tggaacttcg cgggtatcga agcggcggcg agcgcgatcc agggtaacgt gaccagcatc240
cacagcctgc tggatgaagg taaacagagc ctgaccaaac tggcggcggc ctggggtggt300
agcggtagcg aagcgtatca gggtgtgcag cagaaatggg atgcgaccgc gaccgaactg360
aacaacgcgc tgcagaacct ggcgcgtacc atcagcgaag cgggtcaggc gatggcgagc420
accgaaggta acgtgaccgg tatgttcgcg ggatccggca gcggtaatga tgccccggtc480
acgttcagaa catcggaagg tggtgcactg gagtggagct ttaacagcag taccggagct540
ggtgcgctga cacagggaac caccacatat gccatgcacg ggcagcaggg aaatgacctg600
aatgctggta agaacctgat atttcagggg cagaatggtc agattaacct taaggattcg660
gtttctcagg gggcgggttc cctgacgttc cgtgataatt acacagtaac aacctctaac720
ggaagtacct ggaccggtgc cggtattgtt gtggacaacg gggtgtccgt aaactggcag780
gttaatggtg ttaagggcga taacctgcat aaaattggtg aaggtacgct gacggtacag840
ggtacaggta ttaatgaagg tggcctgaag gtcggggacg gaaaggttgt actgaaccag900
caggcggaca ataaaggaca ggtgcaggcg ttcagcagtg ttaatattgc cagtggccgg960
ccgaccgtgg tactgactga tgagcggcag gtaaatccgg ataccgtctc atggggatat1020
cgtgggggca cactggatgt taatggtaac agtctgacgt ttcatcagtt gaaggcggca1080
gattatggtg ccgtgctggc gaataacgtt gataaacggg ccactatcac gctggactat1140
gccctgcggg ctgacaaagt agcactgaat ggctggtcgg aatcaggtaa aggaactgcc1200
ggaaatttat ataaatacaa taacccgtac acaaatacga cggattactt catcctgaag1260
cagagcacct atggttattt ccccacggac cagagcagca acgccacctg ggagtttgtg1320
gggcacagtc agggggatgc acagaaactg gtagctgacc gtttcaatac tgcagggtat1380
ctgtttcacg gacaactgaa aggcaatctg aatgtggaca atcgcctgcc tgaaggcgtt1440
accggtgctc tggtgatgga cggagctgcg gatatctccg gtacattcac ccaggaaaac1500
gggcgtctga cgctgcaggg gcatccggtt atccatgcat acaatactca gtctgtggct1560
gacaaactgg ctgccagtgg agaccattcg gttctgactc agcctacgtc attcagtcag1620
gaggactggg agaaccgcag ttttaccttt gacaggctgt cactgaagaa cactgatttt1680
ggtcttggtc gcaatgccac actgaacaca accatccagg cagataactc cagcgtcacg1740
ctgggcgaca gccgggtatt tatcgacaaa aacgatggcc agggaacagc ctttaccctt1800
gaagaaggca catctgttgc aactaaagat gcagataaaa gtgtcttcaa cggcaccgtc1860
aacctggata atcagtcagt gctgaatatc aatgatatat tcaatggcgg aatacaggcg1920
aacaacagta ccgtgaatat ctcctcagac agtgccgttc tggggaactc aacactgacc1980
agtaccgccc tgaatctgaa caagggagca aatgctctgg ccagtcagag ttttgtttct2040
gacggtccag tgaatatttc tgatgccacc ctgagtctga acagccgtcc tgatgaggta2100
tctcacacac ttttacctgt atacgattat gccggttcat ggaacctgaa gggagacgat2160
gcccgcctga acgtggggcc gtacagtatg ttgtcaggta atatcaatgt tcaggataaa2220
gggactgtca ccctcggagg ggaaggggaa ctgagtcctg acctgactct tcagaatcag2280
atgttgtaca gcctgtttaa cgggtaccgc aatatctgga gcgggagcct gaatgcaccg2340
gatgccaccg tcagcatgac agacacccag tggtcgatga acggaaactc cacggcagga2400
aatatgaaac ttaaccggac aatagtcggt tttaacgggg gaacatcacc gttcacgaca2460
ctgacaacag ataatctgga cgcggttcag tcagcatttg tcatgcgtac agaccttaac2520
aaggcagaca aactggtgat aaacaagtcg gcaacaggtc atgacaacag catctgggtt2580
aacttcctga aaaaaccttc taacaaggac acgcttgata ttccactggt cagcgcacct2640
gaagcgacag ctgataatct gttcagggca tcaacacggg ttgtgggatt cagtgatgtc2700
acccccatcc ttagtgtcag aaaagaggac gggaaaaaag agtgggtcct cgatggttac2760
caggttgcac gtaacgacgg ccagggtaag gctgccgcca cattcatgca catcagctat2820
aacaacttca tcactgaagt taacaacctg aacaaacgca tgggcgattt gagggatatt2880
aatggcgaag ccggtacgtg ggtgcgtctg ctgaacggtt ccggctctgc tgatggcggt2940
ttcactgacc actataccct gctgcagatg ggggctgacc gtaagcacga actgggaagt3000
atggacctgt ttaccggcgt gatggccacc tacactgaca cagatgcgtc agcagacctg3060
tacagcggta aaacaaaatc atggggtggt ggtttctatg ccagtggtct gttccggtcc3120
ggcgcttact ttgatgtgat tgccaaatat attcacaatg aaaacaaata tgacctgaac3180
tttgccggag ctggtaaaca gaacttccgc agccattcac tgtatgcagg tgcagaagtc3240
ggataccgtt atcatctgac agatacgacg tttgttgaac ctcaggcgga actggtctgg3300
ggaagactgc agggccaaac atttaactgg aacgacagtg gaatggatgt ctcaatgcgt3360
cgtaacagcg ttaatcctct ggtaggcaga accggcgttg tttccggtaa aaccttcagt3420
ggtaaggact ggagtctgac agcccgtgcc ggcctgcatt atgagttcga tctgacggac3480
agtgctgacg ttcatctgaa ggatgcagcg ggagaacatc agattaatgg cagaaaagac3540
agtcgtatgc tttacggtgt ggggttaaat gcccggtttg gcgacaatac gcgtctgggg3600
ctggaagttg aacgctctgc atttggtaaa tacaacacag atgatgcgat aaacgctaat3660
attcgttatt cattctga 3678
<210>61
<211>3678
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>61
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccatgac cgaacagcag180
tggaacttcg cgggtatcga agcggcggcg agcgcgatcc agggtaacgt gaccagcatc240
cacagcctgc tggatgaagg taaacagagc ctgaccaaac tggcggcggc ctggggtggt300
agcggtagcg aagcgtatca gggtgtgcag cagaaatggg atgcgaccgc gaccgaactg360
aacaacgcgc tgcagaacct ggcgcgtacc atcagcgaag cgggtcaggc gatggcgagc420
accgaaggta acgtgaccgg tatgttcgcg ggatccggca gcggtaatga tgccccggtc480
acgttcagaa catcggaagg tggtgcactg gagtggagct ttaacagcag taccggagct540
ggtgcgctga cacagggaac caccacatat gccatgcacg ggcagcaggg aaatgacctg600
aatgctggta agaacctgat atttcagggg cagaatggtc agattaacct taaggattcg660
gtttctcagg gggcgggttc cctgacgttc cgtgataatt acacagtaac aacctctaac720
ggaagtacct ggaccggtgc cggtattgtt gtggacaacg gggtgtccgt aaactggcag780
gttaatggtg ttaagggcga taacctgcat aaaattggtg aaggtacgct gacggtacag840
ggtacaggta ttaatgaagg tggcctgaag gtcggggacg gaaaggttgt actgaaccag900
caggcggaca ataaaggaca ggtgcaggcg ttcagcagtg ttaatattgc cagtggccgg960
ccgaccgtgg tactgactga tgagcggcag gtaaatccgg ataccgtctc atggggatat1020
cgtgggggca cactggatgt taatggtaac agtctgacgt ttcatcagtt gaaggcggca1080
gattatggtg ccgtgctggc gaataacgtt gataaacggg ccactatcac gctggactat1140
gccctgcggg ctgacaaagt agcactgaat ggctggtcgg aatcaggtaa aggaactgcc1200
ggaaatttat ataaatacaa taacccgtac acaaatacga cggattactt catcctgaag1260
cagagcacct atggttattt ccccacggac cagagcagca acgccacctg ggagtttgtg1320
gggcacagtc agggggatgc acagaaactg gtagctgacc gtttcaatac tgcagggtat1380
ctgtttcacg gacaactgaa aggcaatctg aatgtggaca atcgcctgcc tgaaggcgtt1440
accggtgctc tggtgatgga cggagctgcg gatatctccg gtacattcac ccaggaaaac1500
gggcgtctga cgctgcaggg gcatccggtt atccatgcat acaatactca gtctgtggct1560
gacaaactgg ctgccagtgg agaccattcg gttctgactc agcctacgtc attcagtcag1620
gaggactggg agaaccgcag ttttaccttt gacaggctgt cactgaagaa cactgatttt1680
ggtcttggtc gcaatgccac actgaacaca accatccagg cagataactc cagcgtcacg1740
ctgggcgaca gccgggtatt tatcgacaaa aacgatggcc agggaacagc ctttaccctt1800
gaagaaggca catctgttgc aactaaagat gcagataaaa gtgtcttcaa cggcaccgtc1860
aacctggata atcagtcagt gctgaatatc aatgatatat tcaatggcgg aatacaggcg1920
aacaacagta ccgtgaatat ctcctcagac agtgccgttc tggggaactc aacactgacc1980
agtaccgccc tgaatctgaa caagggagca aatgctctgg ccagtcagag ttttgtttct2040
gacggtccag tgaatatttc tgatgccacc ctgagtctga acagccgtcc tgatgaggta2100
tctcacacac ttttacctgt atacgattat gccggttcat ggaacctgaa gggagacgat2160
gcccgcctga acgtggggcc gtacagtatg ttgtcaggta atatcaatgt tcaggataaa2220
gggactgtca ccctcggagg ggaaggggaa ctgagtcctg acctgactct tcagaatcag2280
atgttgtaca gcctgtttaa cgggtaccgc aatatctgga gcgggagcct gaatgcaccg2340
gatgccaccg tcagcatgac agacacccag tggtcgatga acggaaactc cacggcagga2400
aatatgaaac ttaaccggac aatagtcggt tttaacgggg gaacatcacc gttcacgaca2460
ctgacaacag ataatctgga cgcggttcag tcagcatttg tcatgcgtac agaccttaac2520
aaggcagaca aactggtgat aaacaagtcg gcaacaggtc atgacaacag catctgggtt2580
aacttcctga aaaaaccttc taacaaggac acgcttgata ttccactggt cagcgcacct2640
gaagcgacag ctgataatct gttcagggca tcaacacggg ttgtgggatt cagtgatgtc2700
acccccatcc ttagtgtcag aaaagaggac gggaaaaaag agtgggtcct cgatggttac2760
caggttgcac gtaacgacgg ccagggtaag gctgccgcca cattcatgca catcagctat2820
aacaacttca tcactgaagt tggttccctg aacaaacgca tgggcgattt gagggatatt2880
aatggcgaag ccggtacgtg ggtgcgtctg ctgaacggtt ccggctctgc tgatggcggt2940
ttcactgacc actataccct gctgcagatg ggggctgacc gtaagcacga actgggaagt3000
atggacctgt ttaccggcgt gatggccacc tacactgaca cagatgcgtc agcagacctg3060
tacagcggta aaacaaaatc atggggtggt ggtttctatg ccagtggtct gttccggtcc3120
ggcgcttact ttgatgtgat tgccaaatat attcacaatg aaaacaaata tgacctgaac3180
tttgccggag ctggtaaaca gaacttccgc agccattcac tgtatgcagg tgcagaagtc3240
ggataccgtt atcatctgac agatacgacg tttgttgaac ctcaggcgga actggtctgg3300
ggaagactgc agggccaaac atttaactgg aacgacagtg gaatggatgt ctcaatgcgt3360
cgtaacagcg ttaatcctct ggtaggcaga accggcgttg tttccggtaa aaccttcagt3420
ggtaaggact ggagtctgac agcccgtgcc ggcctgcatt atgagttcga tctgacggac3480
agtgctgacg ttcatctgaa ggatgcagcg ggagaacatc agattaatgg cagaaaagac3540
agtcgtatgc tttacggtgt ggggttaaat gcccggtttg gcgacaatac gcgtctgggg3600
ctggaagttg aacgctctgc atttggtaaa tacaacacag atgatgcgat aaacgctaat3660
attcgttatt cattctga 3678
<210>62
<211>4227
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>62
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccg gttctgggag ctccatgacc1620
gaacagcagt ggaacttcgc gggtatcgaa gcggcggcga gcgcgatcca gggtaacgtg1680
accagcatcc acagcctgct ggatgaaggt aaacagagcc tgaccaaact ggcggcggcc1740
tggggtggta gcggtagcga agcgtatcag ggtgtgcagc agaaatggga tgcgaccgcg1800
accgaactga acaacgcgct gcagaacctg gcgcgtacca tcagcgaagc gggtcaggcg1860
atggcgagca ccgaaggtaa cgtgaccggt atgttcgcgg gatccggcag cggtaatact1920
gcagggtatc tgtttcacgg acaactgaaa ggcaatctga atgtggacaa tcgcctgcct1980
gaaggcgtta ccggtgctct ggtgatggac ggagctgcgg atatctccgg tacattcacc2040
caggaaaacg ggcgtctgac gctgcagggg catccggtta tccatgcata caatactcag2100
tctgtggctg acaaactggc tgccagtgga gaccattcgg ttctgactca gcctacgtca2160
ttcagtcagg aggactggga gaaccgcagt tttacctttg acaggctgtc actgaagaac2220
actgattttg gtcttggtcg caatgccaca ctgaacacaa ccatccaggc agataactcc2280
agcgtcacgc tgggcgacag ccgggtattt atcgacaaaa acgatggcca gggaacagcc2340
tttacccttg aagaaggcac atctgttgca actaaagatg cagataaaag tgtcttcaac2400
ggcaccgtca acctggataa tcagtcagtg ctgaatatca atgatatatt caatggcgga2460
atacaggcga acaacagtac cgtgaatatc tcctcagaca gtgccgttct ggggaactca2520
acactgacca gtaccgccct gaatctgaac aagggagcaa atgctctggc cagtcagagt2580
tttgtttctg acggtccagt gaatatttct gatgccaccc tgagtctgaa cagccgtcct2640
gatgaggtat ctcacacact tttacctgta tacgattatg ccggttcatg gaacctgaag2700
ggagacgatg cccgcctgaa cgtggggccg tacagtatgt tgtcaggtaa tatcaatgtt2760
caggataaag ggactgtcac cctcggaggg gaaggggaac tgagtcctga cctgactctt2820
cagaatcaga tgttgtacag cctgtttaac gggtaccgca atatctggag cgggagcctg2880
aatgcaccgg atgccaccgt cagcatgaca gacacccagt ggtcgatgaa cggaaactcc2940
acggcaggaa atatgaaact taaccggaca atagtcggtt ttaacggggg aacatcaccg3000
ttcacgacac tgacaacaga taatctggac gcggttcagt cagcatttgt catgcgtaca3060
gaccttaaca aggcagacaa actggtgata aacaagtcgg caacaggtca tgacaacagc3120
atctgggtta acttcctgaa aaaaccttct aacaaggaca cgcttgatat tccactggtc3180
agcgcacctg aagcgacagc tgataatctg ttcagggcat caacacgggt tgtgggattc3240
agtgatgtca cccccatcct tagtgtcaga aaagaggacg ggaaaaaaga gtgggtcctc3300
gatggttacc aggttgcacg taacgacggc cagggtaagg ctgccgccac attcatgcac3360
atcagctata acaacttcat cactgaagtt aacaacctga acaaacgcat gggcgatttg3420
agggatatta atggcgaagc cggtacgtgg gtgcgtctgc tgaacggttc cggctctgct3480
gatggcggtt tcactgacca ctataccctg ctgcagatgg gggctgaccg taagcacgaa3540
ctgggaagta tggacctgtt taccggcgtg atggccacct acactgacac agatgcgtca3600
gcagacctgt acagcggtaa aacaaaatca tggggtggtg gtttctatgc cagtggtctg3660
ttccggtccg gcgcttactt tgatgtgatt gccaaatata ttcacaatga aaacaaatat3720
gacctgaact ttgccggagc tggtaaacag aacttccgca gccattcact gtatgcaggt3780
gcagaagtcg gataccgtta tcatctgaca gatacgacgt ttgttgaacc tcaggcggaa3840
ctggtctggg gaagactgca gggccaaaca tttaactgga acgacagtgg aatggatgtc3900
tcaatgcgtc gtaacagcgt taatcctctg gtaggcagaa ccggcgttgt ttccggtaaa3960
accttcagtg gtaaggactg gagtctgaca gcccgtgccg gcctgcatta tgagttcgat4020
ctgacggaca gtgctgacgt tcatctgaag gatgcagcgg gagaacatca gattaatggc4080
agaaaagaca gtcgtatgct ttacggtgtg gggttaaatg cccggtttgg cgacaatacg4140
cgtctggggc tggaagttga acgctctgca tttggtaaat acaacacaga tgatgcgata4200
aacgctaata ttcgttattc attctga 4227
<210>63
<211>4227
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>63
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccg gttctgggag ctccatgacc1620
gaacagcagt ggaacttcgc gggtatcgaa gcggcggcga gcgcgatcca gggtaacgtg1680
accagcatcc acagcctgct ggatgaaggt aaacagagcc tgaccaaact ggcggcggcc1740
tggggtggta gcggtagcga agcgtatcag ggtgtgcagc agaaatggga tgcgaccgcg1800
accgaactga acaacgcgct gcagaacctg gcgcgtacca tcagcgaagc gggtcaggcg1860
atggcgagca ccgaaggtaa cgtgaccggt atgttcgcgg gatccggcag cggtaatact1920
gcagggtatc tgtttcacgg acaactgaaa ggcaatctga atgtggacaa tcgcctgcct1980
gaaggcgtta ccggtgctct ggtgatggac ggagctgcgg atatctccgg tacattcacc2040
caggaaaacg ggcgtctgac gctgcagggg catccggtta tccatgcata caatactcag2100
tctgtggctg acaaactggc tgccagtgga gaccattcgg ttctgactca gcctacgtca2160
ttcagtcagg aggactggga gaaccgcagt tttacctttg acaggctgtc actgaagaac2220
actgattttg gtcttggtcg caatgccaca ctgaacacaa ccatccaggc agataactcc2280
agcgtcacgc tgggcgacag ccgggtattt atcgacaaaa acgatggcca gggaacagcc2340
tttacccttg aagaaggcac atctgttgca actaaagatg cagataaaag tgtcttcaac2400
ggcaccgtca acctggataa tcagtcagtg ctgaatatca atgatatatt caatggcgga2460
atacaggcga acaacagtac cgtgaatatc tcctcagaca gtgccgttct ggggaactca2520
acactgacca gtaccgccct gaatctgaac aagggagcaa atgctctggc cagtcagagt2580
tttgtttctg acggtccagt gaatatttct gatgccaccc tgagtctgaa cagccgtcct2640
gatgaggtat ctcacacact tttacctgta tacgattatg ccggttcatg gaacctgaag2700
ggagacgatg cccgcctgaa cgtggggccg tacagtatgt tgtcaggtaa tatcaatgtt2760
caggataaag ggactgtcac cctcggaggg gaaggggaac tgagtcctga cctgactctt2820
cagaatcaga tgttgtacag cctgtttaac gggtaccgca atatctggag cgggagcctg2880
aatgcaccgg atgccaccgt cagcatgaca gacacccagt ggtcgatgaa cggaaactcc2940
acggcaggaa atatgaaact taaccggaca atagtcggtt ttaacggggg aacatcaccg3000
ttcacgacac tgacaacaga taatctggac gcggttcagt cagcatttgt catgcgtaca3060
gaccttaaca aggcagacaa actggtgata aacaagtcgg caacaggtca tgacaacagc3120
atctgggtta acttcctgaa aaaaccttct aacaaggaca cgcttgatat tccactggtc3180
agcgcacctg aagcgacagc tgataatctg ttcagggcat caacacgggt tgtgggattc3240
agtgatgtca cccccatcct tagtgtcaga aaagaggacg ggaaaaaaga gtgggtcctc3300
gatggttacc aggttgcacg taacgacggc cagggtaagg ctgccgccac attcatgcac3360
atcagctata acaacttcat cactgaagtt ggttccctga acaaacgcat gggcgatttg3420
agggatatta atggcgaagc cggtacgtgg gtgcgtctgc tgaacggttc cggctctgct3480
gatggcggtt tcactgacca ctataccctg ctgcagatgg gggctgaccg taagcacgaa3540
ctgggaagta tggacctgtt taccggcgtg atggccacct acactgacac agatgcgtca3600
gcagacctgt acagcggtaa aacaaaatca tggggtggtg gtttctatgc cagtggtctg3660
ttccggtccg gcgcttactt tgatgtgatt gccaaatata ttcacaatga aaacaaatat3720
gacctgaact ttgccggagc tggtaaacag aacttccgca gccattcact gtatgcaggt3780
gcagaagtcg gataccgtta tcatctgaca gatacgacgt ttgttgaacc tcaggcggaa3840
ctggtctggg gaagactgca gggccaaaca tttaactgga acgacagtgg aatggatgtc3900
tcaatgcgtc gtaacagcgt taatcctctg gtaggcagaa ccggcgttgt ttccggtaaa3960
accttcagtg gtaaggactg gagtctgaca gcccgtgccg gcctgcatta tgagttcgat4020
ctgacggaca gtgctgacgt tcatctgaag gatgcagcgg gagaacatca gattaatggc4080
agaaaagaca gtcgtatgct ttacggtgtg gggttaaatg cccggtttgg cgacaatacg4140
cgtctggggc tggaagttga acgctctgca tttggtaaat acaacacaga tgatgcgata4200
aacgctaata ttcgttattc attctga 4227
<210>64
<211>4335
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>64
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggagc1980
gggagctcca tgaccgaaca gcagtggaac ttcgcgggta tcgaagcggc ggcgagcgcg2040
atccagggta acgtgaccag catccacagc ctgctggatg aaggtaaaca gagcctgacc2100
aaactggcgg cggcctgggg tggtagcggt agcgaagcgt atcagggtgt gcagcagaaa2160
tgggatgcga ccgcgaccga actgaacaac gcgctgcaga acctggcgcg taccatcagc2220
gaagcgggtc aggcgatggc gagcaccgaa ggtaacgtga ccggtatgtt cgcgggatcc2280
ggcagcggta accgcagttt tacctttgac aggctgtcac tgaagaacac tgattttggt2340
cttggtcgca atgccacact gaacacaacc atccaggcag ataactccag cgtcacgctg2400
ggcgacagcc gggtatttat cgacaaaaac gatggccagg gaacagcctt tacccttgaa2460
gaaggcacat ctgttgcaac taaagatgca gataaaagtg tcttcaacgg caccgtcaac2520
ctggataatc agtcagtgct gaatatcaat gatatattca atggcggaat acaggcgaac2580
aacagtaccg tgaatatctc ctcagacagt gccgttctgg ggaactcaac actgaccagt2640
accgccctga atctgaacaa gggagcaaat gctctggcca gtcagagttt tgtttctgac2700
ggtccagtga atatttctga tgccaccctg agtctgaaca gccgtcctga tgaggtatct2760
cacacacttt tacctgtata cgattatgcc ggttcatgga acctgaaggg agacgatgcc2820
cgcctgaacg tggggccgta cagtatgttg tcaggtaata tcaatgttca ggataaaggg2880
actgtcaccc tcggagggga aggggaactg agtcctgacc tgactcttca gaatcagatg2940
ttgtacagcc tgtttaacgg gtaccgcaat atctggagcg ggagcctgaa tgcaccggat3000
gccaccgtca gcatgacaga cacccagtgg tcgatgaacg gaaactccac ggcaggaaat3060
atgaaactta accggacaat agtcggtttt aacgggggaa catcaccgtt cacgacactg3120
acaacagata atctggacgc ggttcagtca gcatttgtca tgcgtacaga ccttaacaag3180
gcagacaaac tggtgataaa caagtcggca acaggtcatg acaacagcat ctgggttaac3240
ttcctgaaaa aaccttctaa caaggacacg cttgatattc cactggtcag cgcacctgaa3300
gcgacagctg ataatctgtt cagggcatca acacgggttg tgggattcag tgatgtcacc3360
cccatcctta gtgtcagaaa agaggacggg aaaaaagagt gggtcctcga tggttaccag3420
gttgcacgta acgacggcca gggtaaggct gccgccacat tcatgcacat cagctataac3480
aacttcatca ctgaagttaa caacctgaac aaacgcatgg gcgatttgag ggatattaat3540
ggcgaagccg gtacgtgggt gcgtctgctg aacggttccg gctctgctga tggcggtttc3600
actgaccact ataccctgct gcagatgggg gctgaccgta agcacgaact gggaagtatg3660
gacctgttta ccggcgtgat ggccacctac actgacacag atgcgtcagc agacctgtac3720
agcggtaaaa caaaatcatg gggtggtggt ttctatgcca gtggtctgtt ccggtccggc3780
gcttactttg atgtgattgc caaatatatt cacaatgaaa acaaatatga cctgaacttt3840
gccggagctg gtaaacagaa cttccgcagc cattcactgt atgcaggtgc agaagtcgga3900
taccgttatc atctgacaga tacgacgttt gttgaacctc aggcggaact ggtctgggga3960
agactgcagg gccaaacatt taactggaac gacagtggaa tggatgtctc aatgcgtcgt4020
aacagcgtta atcctctggt aggcagaacc ggcgttgttt ccggtaaaac cttcagtggt4080
aaggactgga gtctgacagc ccgtgccggc ctgcattatg agttcgatct gacggacagt4140
gctgacgttc atctgaagga tgcagcggga gaacatcaga ttaatggcag aaaagacagt4200
cgtatgcttt acggtgtggg gttaaatgcc cggtttggcg acaatacgcg tctggggctg4260
gaagttgaac gctctgcatt tggtaaatac aacacagatg atgcgataaa cgctaatatt4320
cgttattcat tctga 4335
<210>65
<211>4359
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>65
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggcat1980
ccggttatcc atgcatacaa tactcagtct gtggctgaca aactggctgc cagtggagac2040
cattcggttc tgactcagcc tacgtcattc agtcaggagg actgggagaa ccgcagtttt2100
acctttgaca ggctgtcact gaagaacact gattttggtc ttggtcgcaa tgccacactg2160
aacacaacca tccaggcaga taactccagc gtcacgctgg gcgacgggag cgggagctcc2220
atgaccgaac agcagtggaa cttcgcgggt atcgaagcgg cggcgagcgc gatccagggt2280
aacgtgacca gcatccacag cctgctggat gaaggtaaac agagcctgac caaactggcg2340
gcggcctggg gtggtagcgg tagcgaagcg tatcagggtg tgcagcagaa atgggatgcg2400
accgcgaccg aactgaacaa cgcgctgcag aacctggcgc gtaccatcag cgaagcgggt2460
caggcgatgg cgagcaccga aggtaacgtg accggtatgt tcgcgggatc cggcagcggt2520
agtgtcttca acggcaccgt caacctggat aatcagtcag tgctgaatat caatgatata2580
ttcaatggcg gaatacaggc gaacaacagt accgtgaata tctcctcaga cagtgccgtt2640
ctggggaact caacactgac cagtaccgcc ctgaatctga acaagggagc aaatgctctg2700
gccagtcaga gttttgtttc tgacggtcca gtgaatattt ctgatgccac cctgagtctg2760
aacagccgtc ctgatgaggt atctcacaca cttttacctg tatacgatta tgccggttca2820
tggaacctga agggagacga tgcccgcctg aacgtggggc cgtacagtat gttgtcaggt2880
aatatcaatg ttcaggataa agggactgtc accctcggag gggaagggga actgagtcct2940
gacctgactc ttcagaatca gatgttgtac agcctgttta acgggtaccg caatatctgg3000
agcgggagcc tgaatgcacc ggatgccacc gtcagcatga cagacaccca gtggtcgatg3060
aacggaaact ccacggcagg aaatatgaaa cttaaccgga caatagtcgg ttttaacggg3120
ggaacatcac cgttcacgac actgacaaca gataatctgg acgcggttca gtcagcattt3180
gtcatgcgta cagaccttaa caaggcagac aaactggtga taaacaagtc ggcaacaggt3240
catgacaaca gcatctgggt taacttcctg aaaaaacctt ctaacaagga cacgcttgat3300
attccactgg tcagcgcacc tgaagcgaca gctgataatc tgttcagggc atcaacacgg3360
gttgtgggat tcagtgatgt cacccccatc cttagtgtca gaaaagagga cgggaaaaaa3420
gagtgggtcc tcgatggtta ccaggttgca cgtaacgacg gccagggtaa ggctgccgcc3480
acattcatgc acatcagcta taacaacttc atcactgaag ttaacaacct gaacaaacgc3540
atgggcgatt tgagggatat taatggcgaa gccggtacgt gggtgcgtct gctgaacggt3600
tccggctctg ctgatggcgg tttcactgac cactataccc tgctgcagat gggggctgac3660
cgtaagcacg aactgggaag tatggacctg tttaccggcg tgatggccac ctacactgac3720
acagatgcgt cagcagacct gtacagcggt aaaacaaaat catggggtgg tggtttctat3780
gccagtggtc tgttccggtc cggcgcttac tttgatgtga ttgccaaata tattcacaat3840
gaaaacaaat atgacctgaa ctttgccgga gctggtaaac agaacttccg cagccattca3900
ctgtatgcag gtgcagaagt cggataccgt tatcatctga cagatacgac gtttgttgaa3960
cctcaggcgg aactggtctg gggaagactg cagggccaaa catttaactg gaacgacagt4020
ggaatggatg tctcaatgcg tcgtaacagc gttaatcctc tggtaggcag aaccggcgtt4080
gtttccggta aaaccttcag tggtaaggac tggagtctga cagcccgtgc cggcctgcat4140
tatgagttcg atctgacgga cagtgctgac gttcatctga aggatgcagc gggagaacat4200
cagattaatg gcagaaaaga cagtcgtatg ctttacggtg tggggttaaa tgcccggttt4260
ggcgacaata cgcgtctggg gctggaagtt gaacgctctg catttggtaa atacaacaca4320
gatgatgcga taaacgctaa tattcgttat tcattctga 4359
<210>66
<211>4383
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>66
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggcat1980
ccggttatcc atgcatacaa tactcagtct gtggctgaca aactggctgc cagtggagac2040
cattcggttc tgactcagcc tacgtcattc agtcaggagg actgggagaa ccgcagtttt2100
acctttgaca ggctgtcact gaagaacact gattttggtc ttggtcgcaa tgccacactg2160
aacacaacca tccaggcaga taactccagc gtcacgctgg gcgacagccg ggtatttatc2220
gacaaaaacg atggccaggg aacagccttt acccttgaag aaggcacatc tgttgcaact2280
aaagatgcag ataaaagtgt cttcaacggc accgtcaacc tggataatca gtcagtgctg2340
aatatcaatg atatattcaa tggcggaata caggcgaaca acagtaccgt gaatatctcc2400
tcagacagtg ccgttctggg gaactcaaca ctgaccagta ccgccctgaa tctgaacaag2460
ggagcaaatg ctctggccag tcagagtttt gtttctgacg gtccagtgaa tatttctgat2520
gccaccctga gtctgaacag ccgtcctgat gaggtatctc acacactttt acctgtatac2580
gattatgccg gttcatggaa cctgaaggga gacgatgccc gcctgaacgt ggggccgtac2640
agtatgttgt caggtaatat caatgttcag gataaaggga ctgtcaccct cggagggagc2700
gggagctcca tgaccgaaca gcagtggaac ttcgcgggta tcgaagcggc ggcgagcgcg2760
atccagggta acgtgaccag catccacagc ctgctggatg aaggtaaaca gagcctgacc2820
aaactggcgg cggcctgggg tggtagcggt agcgaagcgt atcagggtgt gcagcagaaa2880
tgggatgcga ccgcgaccga actgaacaac gcgctgcaga acctggcgcg taccatcagc2940
gaagcgggtc aggcgatggc gagcaccgaa ggtaacgtga ccggtatgtt cgcgggatcc3000
ggcagcgggt accgcaatat ctggagcggg agcctgaatg caccggatgc caccgtcagc3060
atgacagaca cccagtggtc gatgaacgga aactccacgg caggaaatat gaaacttaac3120
cggacaatag tcggttttaa cgggggaaca tcaccgttca cgacactgac aacagataat3180
ctggacgcgg ttcagtcagc atttgtcatg cgtacagacc ttaacaaggc agacaaactg3240
gtgataaaca agtcggcaac aggtcatgac aacagcatct gggttaactt cctgaaaaaa3300
ccttctaaca aggacacgct tgatattcca ctggtcagcg cacctgaagc gacagctgat3360
aatctgttca gggcatcaac acgggttgtg ggattcagtg atgtcacccc catccttagt3420
gtcagaaaag aggacgggaa aaaagagtgg gtcctcgatg gttaccaggt tgcacgtaac3480
gacggccagg gtaaggctgc cgccacattc atgcacatca gctataacaa cttcatcact3540
gaagttaaca acctgaacaa acgcatgggc gatttgaggg atattaatgg cgaagccggt3600
acgtgggtgc gtctgctgaa cggttccggc tctgctgatg gcggtttcac tgaccactat3660
accctgctgc agatgggggc tgaccgtaag cacgaactgg gaagtatgga cctgtttacc3720
ggcgtgatgg ccacctacac tgacacagat gcgtcagcag acctgtacag cggtaaaaca3780
aaatcatggg gtggtggttt ctatgccagt ggtctgttcc ggtccggcgc ttactttgat3840
gtgattgcca aatatattca caatgaaaac aaatatgacc tgaactttgc cggagctggt3900
aaacagaact tccgcagcca ttcactgtat gcaggtgcag aagtcggata ccgttatcat3960
ctgacagata cgacgtttgt tgaacctcag gcggaactgg tctggggaag actgcagggc4020
caaacattta actggaacga cagtggaatg gatgtctcaa tgcgtcgtaa cagcgttaat4080
cctctggtag gcagaaccgg cgttgtttcc ggtaaaacct tcagtggtaa ggactggagt4140
ctgacagccc gtgccggcct gcattatgag ttcgatctga cggacagtgc tgacgttcat4200
ctgaaggatg cagcgggaga acatcagatt aatggcagaa aagacagtcg tatgctttac4260
ggtgtggggt taaatgcccg gtttggcgac aatacgcgtc tggggctgga agttgaacgc4320
tctgcatttg gtaaatacaa cacagatgat gcgataaacg ctaatattcg ttattcattc4380
tga 4383
<210>67
<211>4434
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>67
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggcat1980
ccggttatcc atgcatacaa tactcagtct gtggctgaca aactggctgc cagtggagac2040
cattcggttc tgactcagcc tacgtcattc agtcaggagg actgggagaa ccgcagtttt2100
acctttgaca ggctgtcact gaagaacact gattttggtc ttggtcgcaa tgccacactg2160
aacacaacca tccaggcaga taactccagc gtcacgctgg gcgacagccg ggtatttatc2220
gacaaaaacg atggccaggg aacagccttt acccttgaag aaggcacatc tgttgcaggt2280
tctgggagct ccatgaccga acagcagtgg aacttcgcgg gtatcgaagc ggcggcgagc2340
gcgatccagg gtaacgtgac cagcatccac agcctgctgg atgaaggtaa acagagcctg2400
accaaactgg cggcggcctg gggtggtagc ggtagcgaag cgtatcaggg tgtgcagcag2460
aaatgggatg cgaccgcgac cgaactgaac aacgcgctgc agaacctggc gcgtaccatc2520
agcgaagcgg gtcaggcgat ggcgagcacc gaaggtaacg tgaccggtat gttcgcggga2580
tccggcagcg gtaaaagtgt cttcaacggc accgtcaacc tggataatca gtcagtgctg2640
aatatcaatg atatattcaa tggcggaata caggcgaaca acagtaccgt gaatatctcc2700
tcagacagtg ccgttctggg gaactcaaca ctgaccagta ccgccctgaa tctgaacaag2760
ggagcaaatg ctctggccag tcagagtttt gtttctgacg gtccagtgaa tatttctgat2820
gccaccctga gtctgaacag ccgtcctgat gaggtatctc acacactttt acctgtatac2880
gattatgccg gttcatggaa cctgaaggga gacgatgccc gcctgaacgt ggggccgtac2940
agtatgttgt caggtaatat caatgttcag gataaaggga ctgtcaccct cggaggggaa3000
ggggaactga gtcctgacct gactcttcag aatcagatgt tgtacagcct gtttaacggg3060
taccgcaata tctggagcgg gagcctgaat gcaccggatg ccaccgtcag catgacagac3120
acccagtggt cgatgaacgg aaactccacg gcaggaaata tgaaacttaa ccggacaata3180
gtcggtttta acgggggaac atcaccgttc acgacactga caacagataa tctggacgcg3240
gttcagtcag catttgtcat gcgtacagac cttaacaagg cagacaaact ggtgataaac3300
aagtcggcaa caggtcatga caacagcatc tgggttaact tcctgaaaaa accttctaac3360
aaggacacgc ttgatattcc actggtcagc gcacctgaag cgacagctga taatctgttc3420
agggcatcaa cacgggttgt gggattcagt gatgtcaccc ccatccttag tgtcagaaaa3480
gaggacggga aaaaagagtg ggtcctcgat ggttaccagg ttgcacgtaa cgacggccag3540
ggtaaggctg ccgccacatt catgcacatc agctataaca acttcatcac tgaagttaac3600
aacctgaaca aacgcatggg cgatttgagg gatattaatg gcgaagccgg tacgtgggtg3660
cgtctgctga acggttccgg ctctgctgat ggcggtttca ctgaccacta taccctgctg3720
cagatggggg ctgaccgtaa gcacgaactg ggaagtatgg acctgtttac cggcgtgatg3780
gccacctaca ctgacacaga tgcgtcagca gacctgtaca gcggtaaaac aaaatcatgg3840
ggtggtggtt tctatgccag tggtctgttc cggtccggcg cttactttga tgtgattgcc3900
aaatatattc acaatgaaaa caaatatgac ctgaactttg ccggagctgg taaacagaac3960
ttccgcagcc attcactgta tgcaggtgca gaagtcggat accgttatca tctgacagat4020
acgacgtttg ttgaacctca ggcggaactg gtctggggaa gactgcaggg ccaaacattt4080
aactggaacg acagtggaat ggatgtctca atgcgtcgta acagcgttaa tcctctggta4140
ggcagaaccg gcgttgtttc cggtaaaacc ttcagtggta aggactggag tctgacagcc4200
cgtgccggcc tgcattatga gttcgatctg acggacagtg ctgacgttca tctgaaggat4260
gcagcgggag aacatcagat taatggcaga aaagacagtc gtatgcttta cggtgtgggg4320
ttaaatgccc ggtttggcga caatacgcgt ctggggctgg aagttgaacg ctctgcattt4380
ggtaaataca acacagatga tgcgataaac gctaatattc gttattcatt ctga 4434
<210>68
<211>4449
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>68
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggcat1980
ccggttatcc atgcatacaa tactcagtct gtggctgaca aactggctgc cagtggagac2040
cattcggttc tgactcagcc tacgtcattc agtcaggagg actgggagaa ccgcagtttt2100
acctttgaca ggctgtcact gaagaacact gattttggtc ttggtcgcaa tgccacactg2160
aacacaacca tccaggcaga taactccagc gtcacgctgg gcgacagccg ggtatttatc2220
gacaaaaacg atggccaggg aacagccttt acccttgaag aaggcacatc tgttgcaact2280
aaagatgcag ataaaagtgt cttcaacggc gggagcggga gctccatgac cgaacagcag2340
tggaacttcg cgggtatcga agcggcggcg agcgcgatcc agggtaacgt gaccagcatc2400
cacagcctgc tggatgaagg taaacagagc ctgaccaaac tggcggcggc ctggggtggt2460
agcggtagcg aagcgtatca gggtgtgcag cagaaatggg atgcgaccgc gaccgaactg2520
aacaacgcgc tgcagaacct ggcgcgtacc atcagcgaag cgggtcaggc gatggcgagc2580
accgaaggta acgtgaccgg tatgttcgcg ggatccggca gcggtaccgt caacctggat2640
aatcagtcag tgctgaatat caatgatata ttcaatggcg gaatacaggc gaacaacagt2700
accgtgaata tctcctcaga cagtgccgtt ctggggaact caacactgac cagtaccgcc2760
ctgaatctga acaagggagc aaatgctctg gccagtcaga gttttgtttc tgacggtcca2820
gtgaatattt ctgatgccac cctgagtctg aacagccgtc ctgatgaggt atctcacaca2880
cttttacctg tatacgatta tgccggttca tggaacctga agggagacga tgcccgcctg2940
aacgtggggc cgtacagtat gttgtcaggt aatatcaatg ttcaggataa agggactgtc3000
accctcggag gggaagggga actgagtcct gacctgactc ttcagaatca gatgttgtac3060
agcctgttta acgggtaccg caatatctgg agcgggagcc tgaatgcacc ggatgccacc3120
gtcagcatga cagacaccca gtggtcgatg aacggaaact ccacggcagg aaatatgaaa3180
cttaaccgga caatagtcgg ttttaacggg ggaacatcac cgttcacgac actgacaaca3240
gataatctgg acgcggttca gtcagcattt gtcatgcgta cagaccttaa caaggcagac3300
aaactggtga taaacaagtc ggcaacaggt catgacaaca gcatctgggt taacttcctg3360
aaaaaacctt ctaacaagga cacgcttgat attccactgg tcagcgcacc tgaagcgaca3420
gctgataatc tgttcagggc atcaacacgg gttgtgggat tcagtgatgt cacccccatc3480
cttagtgtca gaaaagagga cgggaaaaaa gagtgggtcc tcgatggtta ccaggttgca3540
cgtaacgacg gccagggtaa ggctgccgcc acattcatgc acatcagcta taacaacttc3600
atcactgaag ttaacaacct gaacaaacgc atgggcgatt tgagggatat taatggcgaa3660
gccggtacgt gggtgcgtct gctgaacggt tccggctctg ctgatggcgg tttcactgac3720
cactataccc tgctgcagat gggggctgac cgtaagcacg aactgggaag tatggacctg3780
tttaccggcg tgatggccac ctacactgac acagatgcgt cagcagacct gtacagcggt3840
aaaacaaaat catggggtgg tggtttctat gccagtggtc tgttccggtc cggcgcttac3900
tttgatgtga ttgccaaata tattcacaat gaaaacaaat atgacctgaa ctttgccgga3960
gctggtaaac agaacttccg cagccattca ctgtatgcag gtgcagaagt cggataccgt4020
tatcatctga cagatacgac gtttgttgaa cctcaggcgg aactggtctg gggaagactg4080
cagggccaaa catttaactg gaacgacagt ggaatggatg tctcaatgcg tcgtaacagc4140
gttaatcctc tggtaggcag aaccggcgtt gtttccggta aaaccttcag tggtaaggac4200
tggagtctga cagcccgtgc cggcctgcat tatgagttcg atctgacgga cagtgctgac4260
gttcatctga aggatgcagc gggagaacat cagattaatg gcagaaaaga cagtcgtatg4320
ctttacggtg tggggttaaa tgcccggttt ggcgacaata cgcgtctggg gctggaagtt4380
gaacgctctg catttggtaa atacaacaca gatgatgcga taaacgctaa tattcgttat4440
tcattctga 4449
<210>69
<211>4275
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>69
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggagc1980
gggagctccg tgatagcggg cgtcgaccag gcgcttgcag caacaggcca ggctagccag2040
cgggcggcag gcgcatctgg tggggtcacc gtcggtgtcg gcgtgggcac ggaacagagg2100
aacctttcgg tggttgcacc gagtcagttc acatttagtt cacgcagccc agattttgtg2160
gatgaaaccg caggtcaatc gtggtgcgcg atactgggat tgaaccagtt tcacggatcc2220
ggcagcggta accgcagttt tacctttgac aggctgtcac tgaagaacac tgattttggt2280
cttggtcgca atgccacact gaacacaacc atccaggcag ataactccag cgtcacgctg2340
ggcgacagcc gggtatttat cgacaaaaac gatggccagg gaacagcctt tacccttgaa2400
gaaggcacat ctgttgcaac taaagatgca gataaaagtg tcttcaacgg caccgtcaac2460
ctggataatc agtcagtgct gaatatcaat gatatattca atggcggaat acaggcgaac2520
aacagtaccg tgaatatctc ctcagacagt gccgttctgg ggaactcaac actgaccagt2580
accgccctga atctgaacaa gggagcaaat gctctggcca gtcagagttt tgtttctgac2640
ggtccagtga atatttctga tgccaccctg agtctgaaca gccgtcctga tgaggtatct2700
cacacacttt tacctgtata cgattatgcc ggttcatgga acctgaaggg agacgatgcc2760
cgcctgaacg tggggccgta cagtatgttg tcaggtaata tcaatgttca ggataaaggg2820
actgtcaccc tcggagggga aggggaactg agtcctgacc tgactcttca gaatcagatg2880
ttgtacagcc tgtttaacgg gtaccgcaat atctggagcg ggagcctgaa tgcaccggat2940
gccaccgtca gcatgacaga cacccagtgg tcgatgaacg gaaactccac ggcaggaaat3000
atgaaactta accggacaat agtcggtttt aacgggggaa catcaccgtt cacgacactg3060
acaacagata atctggacgc ggttcagtca gcatttgtca tgcgtacaga ccttaacaag3120
gcagacaaac tggtgataaa caagtcggca acaggtcatg acaacagcat ctgggttaac3180
ttcctgaaaa aaccttctaa caaggacacg cttgatattc cactggtcag cgcacctgaa3240
gcgacagctg ataatctgtt cagggcatca acacgggttg tgggattcag tgatgtcacc3300
cccatcctta gtgtcagaaa agaggacggg aaaaaagagt gggtcctcga tggttaccag3360
gttgcacgta acgacggcca gggtaaggct gccgccacat tcatgcacat cagctataac3420
aacttcatca ctgaagttaa caacctgaac aaacgcatgg gcgatttgag ggatattaat3480
ggcgaagccg gtacgtgggt gcgtctgctg aacggttccg gctctgctga tggcggtttc3540
actgaccact ataccctgct gcagatgggg gctgaccgta agcacgaact gggaagtatg3600
gacctgttta ccggcgtgat ggccacctac actgacacag atgcgtcagc agacctgtac3660
agcggtaaaa caaaatcatg gggtggtggt ttctatgcca gtggtctgtt ccggtccggc3720
gcttactttg atgtgattgc caaatatatt cacaatgaaa acaaatatga cctgaacttt3780
gccggagctg gtaaacagaa cttccgcagc cattcactgt atgcaggtgc agaagtcgga3840
taccgttatc atctgacaga tacgacgttt gttgaacctc aggcggaact ggtctgggga3900
agactgcagg gccaaacatt taactggaac gacagtggaa tggatgtctc aatgcgtcgt3960
aacagcgtta atcctctggt aggcagaacc ggcgttgttt ccggtaaaac cttcagtggt4020
aaggactgga gtctgacagc ccgtgccggc ctgcattatg agttcgatct gacggacagt4080
gctgacgttc atctgaagga tgcagcggga gaacatcaga ttaatggcag aaaagacagt4140
cgtatgcttt acggtgtggg gttaaatgcc cggtttggcg acaatacgcg tctggggctg4200
gaagttgaac gctctgcatt tggtaaatac aacacagatg atgcgataaa cgctaatatt4260
cgttattcat tctga 4275
<210>70
<211>4299
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>70
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggcat1980
ccggttatcc atgcatacaa tactcagtct gtggctgaca aactggctgc cagtggagac2040
cattcggttc tgactcagcc tacgtcattc agtcaggagg actgggagaa ccgcagtttt2100
acctttgaca ggctgtcact gaagaacact gattttggtc ttggtcgcaa tgccacactg2160
aacacaacca tccaggcaga taactccagc gtcacgctgg gcgacgggag cgggagctcc2220
gtgatagcgg gcgtcgacca ggcgcttgca gcaacaggcc aggctagcca gcgggcggca2280
ggcgcatctg gtggggtcac cgtcggtgtc ggcgtgggca cggaacagag gaacctttcg2340
gtggttgcac cgagtcagtt cacatttagt tcacgcagcc cagattttgt ggatgaaacc2400
gcaggtcaat cgtggtgcgc gatactggga ttgaaccagt ttcacggatc cggcagcggt2460
agtgtcttca acggcaccgt caacctggat aatcagtcag tgctgaatat caatgatata2520
ttcaatggcg gaatacaggc gaacaacagt accgtgaata tctcctcaga cagtgccgtt2580
ctggggaact caacactgac cagtaccgcc ctgaatctga acaagggagc aaatgctctg2640
gccagtcaga gttttgtttc tgacggtcca gtgaatattt ctgatgccac cctgagtctg2700
aacagccgtc ctgatgaggt atctcacaca cttttacctg tatacgatta tgccggttca2760
tggaacctga agggagacga tgcccgcctg aacgtggggc cgtacagtat gttgtcaggt2820
aatatcaatg ttcaggataa agggactgtc accctcggag gggaagggga actgagtcct2880
gacctgactc ttcagaatca gatgttgtac agcctgttta acgggtaccg caatatctgg2940
agcgggagcc tgaatgcacc ggatgccacc gtcagcatga cagacaccca gtggtcgatg3000
aacggaaact ccacggcagg aaatatgaaa cttaaccgga caatagtcgg ttttaacggg3060
ggaacatcac cgttcacgac actgacaaca gataatctgg acgcggttca gtcagcattt3120
gtcatgcgta cagaccttaa caaggcagac aaactggtga taaacaagtc ggcaacaggt3180
catgacaaca gcatctgggt taacttcctg aaaaaacctt ctaacaagga cacgcttgat3240
attccactgg tcagcgcacc tgaagcgaca gctgataatc tgttcagggc atcaacacgg3300
gttgtgggat tcagtgatgt cacccccatc cttagtgtca gaaaagagga cgggaaaaaa3360
gagtgggtcc tcgatggtta ccaggttgca cgtaacgacg gccagggtaa ggctgccgcc3420
acattcatgc acatcagcta taacaacttc atcactgaag ttaacaacct gaacaaacgc3480
atgggcgatt tgagggatat taatggcgaa gccggtacgt gggtgcgtct gctgaacggt3540
tccggctctg ctgatggcgg tttcactgac cactataccc tgctgcagat gggggctgac3600
cgtaagcacg aactgggaag tatggacctg tttaccggcg tgatggccac ctacactgac3660
acagatgcgt cagcagacct gtacagcggt aaaacaaaat catggggtgg tggtttctat3720
gccagtggtc tgttccggtc cggcgcttac tttgatgtga ttgccaaata tattcacaat3780
gaaaacaaat atgacctgaa ctttgccgga gctggtaaac agaacttccg cagccattca3840
ctgtatgcag gtgcagaagt cggataccgt tatcatctga cagatacgac gtttgttgaa3900
cctcaggcgg aactggtctg gggaagactg cagggccaaa catttaactg gaacgacagt3960
ggaatggatg tctcaatgcg tcgtaacagc gttaatcctc tggtaggcag aaccggcgtt4020
gtttccggta aaaccttcag tggtaaggac tggagtctga cagcccgtgc cggcctgcat4080
tatgagttcg atctgacgga cagtgctgac gttcatctga aggatgcagc gggagaacat4140
cagattaatg gcagaaaaga cagtcgtatg ctttacggtg tggggttaaa tgcccggttt4200
ggcgacaata cgcgtctggg gctggaagtt gaacgctctg catttggtaa atacaacaca4260
gatgatgcga taaacgctaa tattcgttat tcattctga 4299
<210>71
<211>4323
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>71
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccc tgcgggctga caaagtagca1620
ctgaatggct ggtcggaatc aggtaaagga actgccggaa atttatataa atacaataac1680
ccgtacacaa atacgacgga ttacttcatc ctgaagcaga gcacctatgg ttatttcccc1740
acggaccaga gcagcaacgc cacctgggag tttgtggggc acagtcaggg ggatgcacag1800
aaactggtag ctgaccgttt caatactgca gggtatctgt ttcacggaca actgaaaggc1860
aatctgaatg tggacaatcg cctgcctgaa ggcgttaccg gtgctctggt gatggacgga1920
gctgcggata tctccggtac attcacccag gaaaacgggc gtctgacgct gcaggggcat1980
ccggttatcc atgcatacaa tactcagtct gtggctgaca aactggctgc cagtggagac2040
cattcggttc tgactcagcc tacgtcattc agtcaggagg actgggagaa ccgcagtttt2100
acctttgaca ggctgtcact gaagaacact gattttggtc ttggtcgcaa tgccacactg2160
aacacaacca tccaggcaga taactccagc gtcacgctgg gcgacagccg ggtatttatc2220
gacaaaaacg atggccaggg aacagccttt acccttgaag aaggcacatc tgttgcaact2280
aaagatgcag ataaaagtgt cttcaacggc accgtcaacc tggataatca gtcagtgctg2340
aatatcaatg atatattcaa tggcggaata caggcgaaca acagtaccgt gaatatctcc2400
tcagacagtg ccgttctggg gaactcaaca ctgaccagta ccgccctgaa tctgaacaag2460
ggagcaaatg ctctggccag tcagagtttt gtttctgacg gtccagtgaa tatttctgat2520
gccaccctga gtctgaacag ccgtcctgat gaggtatctc acacactttt acctgtatac2580
gattatgccg gttcatggaa cctgaaggga gacgatgccc gcctgaacgt ggggccgtac2640
agtatgttgt caggtaatat caatgttcag gataaaggga ctgtcaccct cggagggagc2700
gggagctccg tgatagcggg cgtcgaccag gcgcttgcag caacaggcca ggctagccag2760
cgggcggcag gcgcatctgg tggggtcacc gtcggtgtcg gcgtgggcac ggaacagagg2820
aacctttcgg tggttgcacc gagtcagttc acatttagtt cacgcagccc agattttgtg2880
gatgaaaccg caggtcaatc gtggtgcgcg atactgggat tgaaccagtt tcacggatcc2940
ggcagcgggt accgcaatat ctggagcggg agcctgaatg caccggatgc caccgtcagc3000
atgacagaca cccagtggtc gatgaacgga aactccacgg caggaaatat gaaacttaac3060
cggacaatag tcggttttaa cgggggaaca tcaccgttca cgacactgac aacagataat3120
ctggacgcgg ttcagtcagc atttgtcatg cgtacagacc ttaacaaggc agacaaactg3180
gtgataaaca agtcggcaac aggtcatgac aacagcatct gggttaactt cctgaaaaaa3240
ccttctaaca aggacacgct tgatattcca ctggtcagcg cacctgaagc gacagctgat3300
aatctgttca gggcatcaac acgggttgtg ggattcagtg atgtcacccc catccttagt3360
gtcagaaaag aggacgggaa aaaagagtgg gtcctcgatg gttaccaggt tgcacgtaac3420
gacggccagg gtaaggctgc cgccacattc atgcacatca gctataacaa cttcatcact3480
gaagttaaca acctgaacaa acgcatgggc gatttgaggg atattaatgg cgaagccggt3540
acgtgggtgc gtctgctgaa cggttccggc tctgctgatg gcggtttcac tgaccactat3600
accctgctgc agatgggggc tgaccgtaag cacgaactgg gaagtatgga cctgtttacc3660
ggcgtgatgg ccacctacac tgacacagat gcgtcagcag acctgtacag cggtaaaaca3720
aaatcatggg gtggtggttt ctatgccagt ggtctgttcc ggtccggcgc ttactttgat3780
gtgattgcca aatatattca caatgaaaac aaatatgacc tgaactttgc cggagctggt3840
aaacagaact tccgcagcca ttcactgtat gcaggtgcag aagtcggata ccgttatcat3900
ctgacagata cgacgtttgt tgaacctcag gcggaactgg tctggggaag actgcagggc3960
caaacattta actggaacga cagtggaatg gatgtctcaa tgcgtcgtaa cagcgttaat4020
cctctggtag gcagaaccgg cgttgtttcc ggtaaaacct tcagtggtaa ggactggagt4080
ctgacagccc gtgccggcct gcattatgag ttcgatctga cggacagtgc tgacgttcat4140
ctgaaggatg cagcgggaga acatcagatt aatggcagaa aagacagtcg tatgctttac4200
ggtgtggggt taaatgcccg gtttggcgac aatacgcgtc tggggctgga agttgaacgc4260
tctgcatttg gtaaatacaa cacagatgat gcgataaacg ctaatattcg ttattcattc4320
tga 4323
<210>72
<211>3681
<212>DNA
<213>大肠杆菌 /结核分枝杆菌
<400> 72
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccatgtc gcaaatcatg180
tacaactacc ccgcgatgtt gggtcacgcc ggggatatgg ccggatatgc cggcacgctg240
cagagcttgg gtgccgagat cgccgtggag caggccgcgt tgcagagtgc gtggcagggc300
gataccggga tcacgtatca ggcgtggcag gcacagtgga accaggccat ggaagatttg360
gtgcgggcct atcatgcgat gtccagcacc catgaagcca acaccatggc gatgatggcc420
cgcgacacgg ccgaagccgc caaatggggc ggcggatccg gcagcggtaa tgatgccccg480
gtcacgttca gaacatcgga aggtggtgca ctggagtgga gctttaacag cagtaccgga540
gctggtgcgc tgacacaggg aaccaccaca tatgccatgc acgggcagca gggaaatgac600
ctgaatgctg gtaagaacct gatatttcag gggcagaatg gtcagattaa ccttaaggat660
tcggtttctc agggggcggg ttccctgacg ttccgtgata attacacagt aacaacctct720
aacggaagta cctggaccgg tgccggtatt gttgtggaca acggggtgtc cgtaaactgg780
caggttaatg gtgttaaggg cgataacctg cataaaattg gtgaaggtac gctgacggta840
cagggtacag gtattaatga aggtggcctg aaggtcgggg acggaaaggt tgtactgaac900
cagcaggcgg acaataaagg acaggtgcag gcgttcagca gtgttaatat tgccagtggc960
cggccgaccg tggtactgac tgatgagcgg caggtaaatc cggataccgt ctcatgggga1020
tatcgtgggg gcacactgga tgttaatggt aacagtctga cgtttcatca gttgaaggcg1080
gcagattatg gtgccgtgct ggcgaataac gttgataaac gggccactat cacgctggac1140
tatgccctgc gggctgacaa agtagcactg aatggctggt cggaatcagg taaaggaact1200
gccggaaatt tatataaata caataacccg tacacaaata cgacggatta cttcatcctg1260
aagcagagca cctatggtta tttccccacg gaccagagca gcaacgccac ctgggagttt1320
gtggggcaca gtcaggggga tgcacagaaa ctggtagctg accgtttcaa tactgcaggg1380
tatctgtttc acggacaact gaaaggcaat ctgaatgtgg acaatcgcct gcctgaaggc1440
gttaccggtg ctctggtgat ggacggagct gcggatatct ccggtacatt cacccaggaa1500
aacgggcgtc tgacgctgca ggggcatccg gttatccatg catacaatac tcagtctgtg1560
gctgacaaac tggctgccag tggagaccat tcggttctga ctcagcctac gtcattcagt1620
caggaggact gggagaaccg cagttttacc tttgacaggc tgtcactgaa gaacactgat1680
tttggtcttg gtcgcaatgc cacactgaac acaaccatcc aggcagataa ctccagcgtc1740
acgctgggcg acagccgggt atttatcgac aaaaacgatg gccagggaac agcctttacc1800
cttgaagaag gcacatctgt tgcaactaaa gatgcagata aaagtgtctt caacggcacc1860
gtcaacctgg ataatcagtc agtgctgaat atcaatgata tattcaatgg cggaatacag1920
gcgaacaaca gtaccgtgaa tatctcctca gacagtgccg ttctggggaa ctcaacactg1980
accagtaccg ccctgaatct gaacaaggga gcaaatgctc tggccagtca gagttttgtt2040
tctgacggtc cagtgaatat ttctgatgcc accctgagtc tgaacagccg tcctgatgag2100
gtatctcaca cacttttacc tgtatacgat tatgccggtt catggaacct gaagggagac2160
gatgcccgcc tgaacgtggg gccgtacagt atgttgtcag gtaatatcaa tgttcaggat2220
aaagggactg tcaccctcgg aggggaaggg gaactgagtc ctgacctgac tcttcagaat2280
cagatgttgt acagcctgtt taacgggtac cgcaatatct ggagcgggag cctgaatgca2340
ccggatgcca ccgtcagcat gacagacacc cagtggtcga tgaacggaaa ctccacggca2400
ggaaatatga aacttaaccg gacaatagtc ggttttaacg ggggaacatc accgttcacg2460
acactgacaa cagataatct ggacgcggtt cagtcagcat ttgtcatgcg tacagacctt2520
aacaaggcag acaaactggt gataaacaag tcggcaacag gtcatgacaa cagcatctgg2580
gttaacttcc tgaaaaaacc ttctaacaag gacacgcttg atattccact ggtcagcgca2640
cctgaagcga cagctgataa tctgttcagg gcatcaacac gggttgtggg attcagtgat2700
gtcaccccca tccttagtgt cagaaaagag gacgggaaaa aagagtgggt cctcgatggt2760
taccaggttg cacgtaacga cggccagggt aaggctgccg ccacattcat gcacatcagc2820
tataacaact tcatcactga agttaacaac ctgaacaaac gcatgggcga tttgagggat2880
attaatggcg aagccggtac gtgggtgcgt ctgctgaacg gttccggctc tgctgatggc2940
ggtttcactg accactatac cctgctgcag atgggggctg accgtaagca cgaactggga3000
agtatggacc tgtttaccgg cgtgatggcc acctacactg acacagatgc gtcagcagac3060
ctgtacagcg gtaaaacaaa atcatggggt ggtggtttct atgccagtgg tctgttccgg3120
tccggcgctt actttgatgt gattgccaaa tatattcaca atgaaaacaa atatgacctg3180
aactttgccg gagctggtaa acagaacttc cgcagccatt cactgtatgc aggtgcagaa3240
gtcggatacc gttatcatct gacagatacg acgtttgttg aacctcaggc ggaactggtc3300
tggggaagac tgcagggcca aacatttaac tggaacgaca gtggaatgga tgtctcaatg3360
cgtcgtaaca gcgttaatcc tctggtaggc agaaccggcg ttgtttccgg taaaaccttc3420
agtggtaagg actggagtct gacagcccgt gccggcctgc attatgagtt cgatctgacg3480
gacagtgctg acgttcatct gaaggatgca gcgggagaac atcagattaa tggcagaaaa3540
gacagtcgta tgctttacgg tgtggggtta aatgcccggt ttggcgacaa tacgcgtctg3600
gggctggaag ttgaacgctc tgcatttggt aaatacaaca cagatgatgc gataaacgct3660
aatattcgtt attcattctg a 3681
<210>73
<211>4230
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>73
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggaa cggtcaataa tgaactcggg180
tatcagttat ttcgtgattt tgctgaaaat aaggggatgt tccgcccggg ggcaacgaat240
atcgctattt ataataagca gggagaattt gtcggtacgc tggataaggc agctatgcct300
gatttcagtg ctgtggattc ggaaatcggt gtggcgacac tgataaaccc gcagtatatc360
gccagcgtga aacataacgg gggatataca aacgttagct ttggtgatgg tgaaaaccgt420
tacaatatcg tggaccggaa taatgcgccg tcactggatt ttcatgcccc ccggctggat480
aaactggtga cagaggttgc ccctactgcg gtgacggcgc agggggcagt ggctggcgca540
tatctggata aggagcgcta tcctgttttt tatcgtctgg ggtctggtac tcagtatatt600
aaggacagta acggacagct gacaaaaatg ggaggtgcat attcctggct gaccggcggg660
actgtcggta gcctgtcatc ctatcagaat ggagaaatga ttagcaccag ttcaggtctg720
gtttttgatt acaaacttaa tggtgcaatg cccatttatg gcgaggccgg tgacagcggt780
tcgcctttat ttgcttttga tactgttcag aataaatggg tgctggtcgg tgttcttact840
gcggggaatg gcgcgggggg caggggaaat aactgggctg ttattccact ggattttatc900
gggcagaaat ttaatgaaga caatgatgcc ccggtcacgt tcagaacatc ggaaggtggt960
gcactggagt ggagctttaa cagcagtacc ggagctggtg cgctgacaca gggaaccacc1020
acatatgcca tgcacgggca gcagggaaat gacctgaatg ctggtaagaa cctgatattt1080
caggggcaga atggtcagat taaccttaag gattcggttt ctcagggggc gggttccctg1140
acgttccgtg ataattacac agtaacaacc tctaacggaa gtacctggac cggtgccggt1200
attgttgtgg acaacggggt gtccgtaaac tggcaggtta atggtgttaa gggcgataac1260
ctgcataaaa ttggtgaagg tacgctgacg gtacagggta caggtattaa tgaaggtggc1320
ctgaaggtcg gggacggaaa ggttgtactg aaccagcagg cggacaataa aggacaggtg1380
caggcgttca gcagtgttaa tattgccagt ggccggccga ccgtggtact gactgatgag1440
cggcaggtaa atccggatac cgtctcatgg ggatatcgtg ggggcacact ggatgttaat1500
ggtaacagtc tgacgtttca tcagttgaag gcggcagatt atggtgccgt gctggcgaat1560
aacgttgata aacgggccac tatcacgctg gactatgccg gttctgggag ctccatgtcg1620
cagattatgt acaactatcc ggcgatgatg gctcatgccg gggacatggc cggttatgcg1680
ggcacgctgc agagcttggg ggccgatatc gccagtgagc aggccgtgct gtccagtgct1740
tggcagggtg ataccgggat cacgtatcag ggctggcaga cccagtggaa ccaggcccta1800
gaggatctgg tgcgggccta tcagtcgatg tctggcaccc atgagtccaa caccatggcg1860
atgttggctc gagatggggc cgaagccgcc aagtggggcg gcggatccgg cagcggtaat1920
actgcagggt atctgtttca cggacaactg aaaggcaatc tgaatgtgga caatcgcctg1980
cctgaaggcg ttaccggtgc tctggtgatg gacggagctg cggatatctc cggtacattc2040
acccaggaaa acgggcgtct gacgctgcag gggcatccgg ttatccatgc atacaatact2100
cagtctgtgg ctgacaaact ggctgccagt ggagaccatt cggttctgac tcagcctacg2160
tcattcagtc aggaggactg ggagaaccgc agttttacct ttgacaggct gtcactgaag2220
aacactgatt ttggtcttgg tcgcaatgcc acactgaaca caaccatcca ggcagataac2280
tccagcgtca cgctgggcga cagccgggta tttatcgaca aaaacgatgg ccagggaaca2340
gcctttaccc ttgaagaagg cacatctgtt gcaactaaag atgcagataa aagtgtcttc2400
aacggcaccg tcaacctgga taatcagtca gtgctgaata tcaatgatat attcaatggc2460
ggaatacagg cgaacaacag taccgtgaat atctcctcag acagtgccgt tctggggaac2520
tcaacactga ccagtaccgc cctgaatctg aacaagggag caaatgctct ggccagtcag2580
agttttgttt ctgacggtcc agtgaatatt tctgatgcca ccctgagtct gaacagccgt2640
cctgatgagg tatctcacac acttttacct gtatacgatt atgccggttc atggaacctg2700
aagggagacg atgcccgcct gaacgtgggg ccgtacagta tgttgtcagg taatatcaat2760
gttcaggata aagggactgt caccctcgga ggggaagggg aactgagtcc tgacctgact2820
cttcagaatc agatgttgta cagcctgttt aacgggtacc gcaatatctg gagcgggagc2880
ctgaatgcac cggatgccac cgtcagcatg acagacaccc agtggtcgat gaacggaaac2940
tccacggcag gaaatatgaa acttaaccgg acaatagtcg gttttaacgg gggaacatca3000
ccgttcacga cactgacaac agataatctg gacgcggttc agtcagcatt tgtcatgcgt3060
acagacctta acaaggcaga caaactggtg ataaacaagt cggcaacagg tcatgacaac3120
agcatctggg ttaacttcct gaaaaaacct tctaacaagg acacgcttga tattccactg3180
gtcagcgcac ctgaagcgac agctgataat ctgttcaggg catcaacacg ggttgtggga3240
ttcagtgatg tcacccccat ccttagtgtc agaaaagagg acgggaaaaa agagtgggtc3300
ctcgatggtt accaggttgc acgtaacgac ggccagggta aggctgccgc cacattcatg3360
cacatcagct ataacaactt catcactgaa gttaacaacc tgaacaaacg catgggcgat3420
ttgagggata ttaatggcga agccggtacg tgggtgcgtc tgctgaacgg ttccggctct3480
gctgatggcg gtttcactga ccactatacc ctgctgcaga tgggggctga ccgtaagcac3540
gaactgggaa gtatggacct gtttaccggc gtgatggcca cctacactga cacagatgcg3600
tcagcagacc tgtacagcgg taaaacaaaa tcatggggtg gtggtttcta tgccagtggt3660
ctgttccggt ccggcgctta ctttgatgtg attgccaaat atattcacaa tgaaaacaaa3720
tatgacctga actttgccgg agctggtaaa cagaacttcc gcagccattc actgtatgca3780
ggtgcagaag tcggataccg ttatcatctg acagatacga cgtttgttga acctcaggcg3840
gaactggtct ggggaagact gcagggccaa acatttaact ggaacgacag tggaatggat3900
gtctcaatgc gtcgtaacag cgttaatcct ctggtaggca gaaccggcgt tgtttccggt3960
aaaaccttca gtggtaagga ctggagtctg acagcccgtg ccggcctgca ttatgagttc4020
gatctgacgg acagtgctga cgttcatctg aaggatgcag cgggagaaca tcagattaat4080
ggcagaaaag acagtcgtat gctttacggt gtggggttaa atgcccggtt tggcgacaat4140
acgcgtctgg ggctggaagt tgaacgctct gcatttggta aatacaacac agatgatgcg4200
ataaacgcta atattcgtta ttcattctga 4230
<210>74
<211>3552
<212>DNA
<213>大肠杆菌/智人
<400>74
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccaattc tgattctgag180
agtccattgt ctcacgatgg ttacagcttg catgatggtg tttcaatgta cattgaagca240
ttggataagt atgcttctaa ctctgttgtt ggttacattg gtgaaagatc tcaatataga300
gatttgaagt ggtgggaatt gagaggatcc ggcagcggta atgatgcccc ggtcacgttc360
agaacatcgg aaggtggtgc actggagtgg agctttaaca gcagtaccgg agctggtgcg420
ctgacacagg gaaccaccac atatgccatg cacgggcagc agggaaatga cctgaatgct480
ggtaagaacc tgatatttca ggggcagaat ggtcagatta accttaagga ttcggtttct540
cagggggcgg gttccctgac gttccgtgat aattacacag taacaacctc taacggaagt600
acctggaccg gtgccggtat tgttgtggac aacggggtgt ccgtaaactg gcaggttaat660
ggtgttaagg gcgataacct gcataaaatt ggtgaaggta cgctgacggt acagggtaca720
ggtattaatg aaggtggcct gaaggtcggg gacggaaagg ttgtactgaa ccagcaggcg780
gacaataaag gacaggtgca ggcgttcagc agtgttaata ttgccagtgg ccggccgacc840
gtggtactga ctgatgagcg gcaggtaaat ccggataccg tctcatgggg atatcgtggg900
ggcacactgg atgttaatgg taacagtctg acgtttcatc agttgaaggc ggcagattat960
ggtgccgtgc tggcgaataa cgttgataaa cgggccacta tcacgctgga ctatgccctg1020
cgggctgaca aagtagcact gaatggctgg tcggaatcag gtaaaggaac tgccggaaat1080
ttatataaat acaataaccc gtacacaaat acgacggatt acttcatcct gaagcagagc1140
acctatggtt atttccccac ggaccagagc agcaacgcca cctgggagtt tgtggggcac1200
agtcaggggg atgcacagaa actggtagct gaccgtttca atactgcagg gtatctgttt1260
cacggacaac tgaaaggcaa tctgaatgtg gacaatcgcc tgcctgaagg cgttaccggt1320
gctctggtga tggacggagc tgcggatatc tccggtacat tcacccagga aaacgggcgt1380
ctgacgctgc aggggcatcc ggttatccat gcatacaata ctcagtctgt ggctgacaaa1440
ctggctgcca gtggagacca ttcggttctg actcagccta cgtcattcag tcaggaggac1500
tgggagaacc gcagttttac ctttgacagg ctgtcactga agaacactga ttttggtctt1560
ggtcgcaatg ccacactgaa cacaaccatc caggcagata actccagcgt cacgctgggc1620
gacagccggg tatttatcga caaaaacgat ggccagggaa cagcctttac ccttgaagaa1680
ggcacatctg ttgcaactaa agatgcagat aaaagtgtct tcaacggcac cgtcaacctg1740
gataatcagt cagtgctgaa tatcaatgat atattcaatg gcggaataca ggcgaacaac1800
agtaccgtga atatctcctc agacagtgcc gttctgggga actcaacact gaccagtacc1860
gccctgaatc tgaacaaggg agcaaatgct ctggccagtc agagttttgt ttctgacggt1920
ccagtgaata tttctgatgc caccctgagt ctgaacagcc gtcctgatga ggtatctcac1980
acacttttac ctgtatacga ttatgccggt tcatggaacc tgaagggaga cgatgcccgc2040
ctgaacgtgg ggccgtacag tatgttgtca ggtaatatca atgttcagga taaagggact2100
gtcaccctcg gaggggaagg ggaactgagt cctgacctga ctcttcagaa tcagatgttg2160
tacagcctgt ttaacgggta ccgcaatatc tggagcggga gcctgaatgc accggatgcc2220
accgtcagca tgacagacac ccagtggtcg atgaacggaa actccacggc aggaaatatg2280
aaacttaacc ggacaatagt cggttttaac gggggaacat caccgttcac gacactgaca2340
acagataatc tggacgcggt tcagtcagca tttgtcatgc gtacagacct taacaaggca2400
gacaaactgg tgataaacaa gtcggcaaca ggtcatgaca acagcatctg ggttaacttc2460
ctgaaaaaac cttctaacaa ggacacgctt gatattccac tggtcagcgc acctgaagcg2520
acagctgata atctgttcag ggcatcaaca cgggttgtgg gattcagtga tgtcaccccc2580
atccttagtg tcagaaaaga ggacgggaaa aaagagtggg tcctcgatgg ttaccaggtt2640
gcacgtaacg acggccaggg taaggctgcc gccacattca tgcacatcag ctataacaac2700
ttcatcactg aagttaacaa cctgaacaaa cgcatgggcg atttgaggga tattaatggc2760
gaagccggta cgtgggtgcg tctgctgaac ggttccggct ctgctgatgg cggtttcact2820
gaccactata ccctgctgca gatgggggct gaccgtaagc acgaactggg aagtatggac2880
ctgtttaccg gcgtgatggc cacctacact gacacagatg cgtcagcaga cctgtacagc2940
ggtaaaacaa aatcatgggg tggtggtttc tatgccagtg gtctgttccg gtccggcgct3000
tactttgatg tgattgccaa atatattcac aatgaaaaca aatatgacct gaactttgcc3060
ggagctggta aacagaactt ccgcagccat tcactgtatg caggtgcaga agtcggatac3120
cgttatcatc tgacagatac gacgtttgtt gaacctcagg cggaactggt ctggggaaga3180
ctgcagggcc aaacatttaa ctggaacgac agtggaatgg atgtctcaat gcgtcgtaac3240
agcgttaatc ctctggtagg cagaaccggc gttgtttccg gtaaaacctt cagtggtaag3300
gactggagtc tgacagcccg tgccggcctg cattatgagt tcgatctgac ggacagtgct3360
gacgttcatc tgaaggatgc agcgggagaa catcagatta atggcagaaa agacagtcgt3420
atgctttacg gtgtggggtt aaatgcccgg tttggcgaca atacgcgtct ggggctggaa3480
gttgaacgct ctgcatttgg taaatacaac acagatgatg cgataaacgc taatattcgt3540
tattcattct ga 3552
<210>75
<211>4083
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>75
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccttctc ccggccgggg180
ctgccggtcg agtacctgca ggtgccgtcg ccgtcgatgg gccgcgacat caaggttcag240
ttccagagcg gtgggaacaa ctcacctgcg gtttatctgc tcgacggcct gcgcgcccaa300
gacgactaca acggctggga tatcaacacc ccggcgttcg agtggtacta ccagtcggga360
ctgtcgatag tcatgccggt cggcgggcag tccagcttct acagcgactg gtacagcccg420
gcctgcggta aggctggctg ccagacttac aagtgggaaa ccttcctgac cagcgagctg480
ccgcaatggt tgtccgccaa cagggccgtg aagcccaccg gcagcgctgc aatcggcttg540
tcgggatccg gcagcggtaa tgatgccccg gtcacgttca gaacatcgga aggtggtgca600
ctggagtgga gctttaacag cagtaccgga gctggtgcgc tgacacaggg aaccaccaca660
tatgccatgc acgggcagca gggaaatgac ctgaatgctg gtaagaacct gatatttcag720
gggcagaatg gtcagattaa ccttaaggat tcggtttctc agggggcggg ttccctgacg780
ttccgtgata attacacagt aacaacctct aacggaagta cctggaccgg tgccggtatt840
gttgtggaca acggggtgtc cgtaaactgg caggttaatg gtgttaaggg cgataacctg900
cataaaattg gtgaaggtac gctgacggta cagggtacag gtattaatga aggtggcctg960
aaggtcgggg acggaaaggt tgtactgaac cagcaggcgg acaataaagg acaggtgcag1020
gcgttcagca gtgttaatat tgccagtggc cggccgaccg tggtactgac tgatgagcgg1080
caggtaaatc cggataccgt ctcatgggga tatcgtgggg gcacactgga tgttaatggt1140
aacagtctga cgtttcatca gttgaaggcg gcagattatg gtgccgtgct ggcgaataac1200
gttgataaac gggccactat cacgctggac tatgccggtt ctgggagctc caccggcagc1260
gctgcaatcg gcttgtcgat ggccggctcg tcggcaatga tcttggccgc ctaccacccc1320
cagcagttca tctacgccgg ctcgctgtcg gccctgctgg acccctctca ggggatgggg1380
cctagcctga tcggcctcgc gatgggtgac gccggcggtt acaaggccgc agacatgtgg1440
ggtccctcga gtgacccggc atgggagcgc aacgacccta cgcagcagat ccccaagctg1500
gtcgcaaaca acacccggct atgggtttat tgcgggaacg gcaccccgaa cgagttgggc1560
ggtgccaaca tacccgccga gttcttggag aacttcgttc gtagcagcaa cctgaagttc1620
caggatgcgt acaacgccgc gggcgggcac aacgccgtgt tcaacttccc gcccaacggc1680
acgcacagct gggagtactg gggcgctcag ctcaacgcca tgaagggtga cctgcagagt1740
tcgttaggcg ccggcggatc cggcagcggt aatactgcag ggtatctgtt tcacggacaa1800
ctgaaaggca atctgaatgt ggacaatcgc ctgcctgaag gcgttaccgg tgctctggtg1860
atggacggag ctgcggatat ctccggtaca ttcacccagg aaaacgggcg tctgacgctg1920
caggggcatc cggttatcca tgcatacaat actcagtctg tggctgacaa actggctgcc1980
agtggagacc attcggttct gactcagcct acgtcattca gtcaggagga ctgggagaac2040
cgcagtttta cctttgacag gctgtcactg aagaacactg attttggtct tggtcgcaat2100
gccacactga acacaaccat ccaggcagat aactccagcg tcacgctggg cgacagccgg2160
gtatttatcg acaaaaacga tggccaggga acagccttta cccttgaaga aggcacatct2220
gttgcaacta aagatgcaga taaaagtgtc ttcaacggca ccgtcaacct ggataatcag2280
tcagtgctga atatcaatga tatattcaat ggcggaatac aggcgaacaa cagtaccgtg2340
aatatctcct cagacagtgc cgttctgggg aactcaacac tgaccagtac cgccctgaat2400
ctgaacaagg gagcaaatgc tctggccagt cagagttttg tttctgacgg tccagtgaat2460
atttctgatg ccaccctgag tctgaacagc cgtcctgatg aggtatctca cacactttta2520
cctgtatacg attatgccgg ttcatggaac ctgaagggag acgatgcccg cctgaacgtg2580
gggccgtaca gtatgttgtc aggtaatatc aatgttcagg ataaagggac tgtcaccctc2640
ggaggggaag gggaactgag tcctgacctg actcttcaga atcagatgtt gtacagcctg2700
tttaacgggt accgcaatat ctggagcggg agcctgaatg caccggatgc caccgtcagc2760
atgacagaca cccagtggtc gatgaacgga aactccacgg caggaaatat gaaacttaac2820
cggacaatag tcggttttaa cgggggaaca tcaccgttca cgacactgac aacagataat2880
ctggacgcgg ttcagtcagc atttgtcatg cgtacagacc ttaacaaggc agacaaactg2940
gtgataaaca agtcggcaac aggtcatgac aacagcatct gggttaactt cctgaaaaaa3000
ccttctaaca aggacacgct tgatattcca ctggtcagcg cacctgaagc gacagctgat3060
aatctgttca gggcatcaac acgggttgtg ggattcagtg atgtcacccc catccttagt3120
gtcagaaaag aggacgggaa aaaagagtgg gtcctcgatg gttaccaggt tgcacgtaac3180
gacggccagg gtaaggctgc cgccacattc atgcacatca gctataacaa cttcatcact3240
gaagttaaca acctgaacaa acgcatgggc gatttgaggg atattaatgg cgaagccggt3300
acgtgggtgc gtctgctgaa cggttccggc tctgctgatg gcggtttcac tgaccactat3360
accctgctgc agatgggggc tgaccgtaag cacgaactgg gaagtatgga cctgtttacc3420
ggcgtgatgg ccacctacac tgacacagat gcgtcagcag acctgtacag cggtaaaaca3480
aaatcatggg gtggtggttt ctatgccagt ggtctgttcc ggtccggcgc ttactttgat3540
gtgattgcca aatatattca caatgaaaac aaatatgacc tgaactttgc cggagctggt3600
aaacagaact tccgcagcca ttcactgtat gcaggtgcag aagtcggata ccgttatcat3660
ctgacagata cgacgtttgt tgaacctcag gcggaactgg tctggggaag actgcagggc3720
caaacattta actggaacga cagtggaatg gatgtctcaa tgcgtcgtaa cagcgttaat3780
cctctggtag gcagaaccgg cgttgtttcc ggtaaaacct tcagtggtaa ggactggagt3840
ctgacagccc gtgccggcct gcattatgag ttcgatctga cggacagtgc tgacgttcat3900
ctgaaggatg cagcgggaga acatcagatt aatggcagaa aagacagtcg tatgctttac3960
ggtgtggggt taaatgcccg gtttggcgac aatacgcgtc tggggctgga agttgaacgc4020
tctgcatttg gtaaatacaa cacagatgat gcgataaacg ctaatattcg ttattcattc4080
tga 4083
<210>76
<211>4083
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>76
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccttctc ccggccgggg180
ctgccggtcg agtacctgca ggtgccgtcg ccgtcgatgg gccgcgacat caaggttcag240
ttccagagcg gtgggaacaa ctcacctgcg gtttatctgc tcgacggcct gcgcgcccaa300
gacgactaca acggctggga tatcaacacc ccggcgttcg agtggtacta ccagtcggga360
ctgtcgatag tcatgccggt cggcgggcag tccagcttct acagcgactg gtacagcccg420
gcctgcggta aggctggctg ccagacttac aagtgggaaa ccttcctgac cagcgagctg480
ccgcaatggt tgtccgccaa cagggccgtg aagcccaccg gcagcgctgc aatcggcttg540
tcgggatccg gcagcggtaa tgatgccccg gtcacgttca gaacatcgga aggtggtgca600
ctggagtgga gctttaacag cagtaccgga gctggtgcgc tgacacaggg aaccaccaca660
tatgccatgc acgggcagca gggaaatgac ctgaatgctg gtaagaacct gatatttcag720
gggcagaatg gtcagattaa ccttaaggat tcggtttctc agggggcggg ttccctgacg780
ttccgtgata attacacagt aacaacctct aacggaagta cctggaccgg tgccggtatt840
gttgtggaca acggggtgtc cgtaaactgg caggttaatg gtgttaaggg cgataacctg900
cataaaattg gtgaaggtac gctgacggta cagggtacag gtattaatga aggtggcctg960
aaggtcgggg acggaaaggt tgtactgaac cagcaggcgg acaataaagg acaggtgcag1020
gcgttcagca gtgttaatat tgccagtggc cggccgaccg tggtactgac tgatgagcgg1080
caggtaaatc cggataccgt ctcatgggga tatcgtgggg gcacactgga tgttaatggt1140
aacagtctga cgtttcatca gttgaaggcg gcagattatg gtgccgtgct ggcgaataac1200
gttgataaac gggccactat cacgctggac tatgccggtt ctgggagctc caccggcagc1260
gctgcaatcg gcttgtcgat ggccggctcg tcggcaatga tcttggccgc ctaccacccc1320
cagcagttca tctacgccgg ctcgctgtcg gccctgctgg acccctctca ggggatgggg1380
cctagcctga tcggcctcgc gatgggtgac gccggcggtt acaaggccgc agacatgtgg1440
ggtccctcga gtgacccggc atgggagcgc aacgacccta cgcagcagat ccccaagctg1500
gtcgcaaaca acacccggct atgggtttat tgcgggaacg gcaccccgaa cgagttgggc1560
ggtgccaaca tacccgccga gttcttggag aacttcgttc gtagcagcaa cctgaagttc1620
caggatgcgt acaacgccgc gggcgggcac aacgccgtgt tcaacttccc gcccaacggc1680
acgcacagct gggagtactg gggcgctcag ctcaacgcca tgaagggtga cctgcagagt1740
tcgttaggcg ccggcggatc cggcagcggt aatactgcag ggtatctgtt tcacggacaa1800
ctgaaaggca atctgaatgt ggacaatcgc ctgcctgaag gcgttaccgg tgctctggtg1860
atggacggag ctgcggatat ctccggtaca ttcacccagg aaaacgggcg tctgacgctg1920
caggggcatc cggttatcca tgcatacaat actcagtctg tggctgacaa actggctgcc1980
agtggagacc attcggttct gactcagcct acgtcattca gtcaggagga ctgggagaac2040
cgcagtttta cctttgacag gctgtcactg aagaacactg attttggtct tggtcgcaat2100
gccacactga acacaaccat ccaggcagat aactccagcg tcacgctggg cgacagccgg2160
gtatttatcg acaaaaacga tggccaggga acagccttta cccttgaaga aggcacatct2220
gttgcaacta aagatgcaga taaaagtgtc ttcaacggca ccgtcaacct ggataatcag2280
tcagtgctga atatcaatga tatattcaat ggcggaatac aggcgaacaa cagtaccgtg2340
aatatctcct cagacagtgc cgttctgggg aactcaacac tgaccagtac cgccctgaat2400
ctgaacaagg gagcaaatgc tctggccagt cagagttttg tttctgacgg tccagtgaat2460
atttctgatg ccaccctgag tctgaacagc cgtcctgatg aggtatctca cacactttta2520
cctgtatacg attatgccgg ttcatggaac ctgaagggag acgatgcccg cctgaacgtg2580
gggccgtaca gtatgttgtc aggtaatatc aatgttcagg ataaagggac tgtcaccctc2640
ggaggggaag gggaactgag tcctgacctg actcttcaga atcagatgtt gtacagcctg2700
tttaacgggt accgcaatat ctggagcggg agcctgaatg caccggatgc caccgtcagc2760
atgacagaca cccagtggtc gatgaacgga aactccacgg caggaaatat gaaacttaac2820
cggacaatag tcggttttaa cgggggaaca tcaccgttca cgacactgac aacagataat2880
ctggacgcgg ttcagtcagc atttgtcatg cgtacagacc ttaacaaggc agacaaactg2940
gtgataaaca agtcggcaac aggtcatgac aacagcatct gggttaactt cctgaaaaaa3000
ccttctaaca aggacacgct tgatattcca ctggtcagcg cacctgaagc gacagctgat3060
aatctgttca gggcatcaac acgggttgtg ggattcagtg atgtcacccc catccttagt3120
gtcagaaaag aggacgggaa aaaagagtgg gtcctcgatg gttaccaggt tgcacgtaac3180
gacggccagg gtaaggctgc cgccacattc atgcacatca gctataacaa cttcatcact3240
gaagttggtt ccctgaacaa acgcatgggc gatttgaggg atattaatgg cgaagccggt3300
acgtgggtgc gtctgctgaa cggttccggc tctgctgatg gcggtttcac tgaccactat3360
accctgctgc agatgggggc tgaccgtaag cacgaactgg gaagtatgga cctgtttacc3420
ggcgtgatgg ccacctacac tgacacagat gcgtcagcag acctgtacag cggtaaaaca3480
aaatcatggg gtggtggttt ctatgccagt ggtctgttcc ggtccggcgc ttactttgat3540
gtgattgcca aatatattca caatgaaaac aaatatgacc tgaactttgc cggagctggt3600
aaacagaact tccgcagcca ttcactgtat gcaggtgcag aagtcggata ccgttatcat3660
ctgacagata cgacgtttgt tgaacctcag gcggaactgg tctggggaag actgcagggc3720
caaacattta actggaacga cagtggaatg gatgtctcaa tgcgtcgtaa cagcgttaat3780
cctctggtag gcagaaccgg cgttgtttcc ggtaaaacct tcagtggtaa ggactggagt3840
ctgacagccc gtgccggcct gcattatgag ttcgatctga cggacagtgc tgacgttcat3900
ctgaaggatg cagcgggaga acatcagatt aatggcagaa aagacagtcg tatgctttac3960
ggtgtggggt taaatgcccg gtttggcgac aatacgcgtc tggggctgga agttgaacgc4020
tctgcatttg gtaaatacaa cacagatgat gcgataaacg ctaatattcg ttattcattc4080
tga 4083
<210>77
<211>4083
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>77
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccaccgg cagcgctgca180
atcggcttgt cgatggccgg ctcgtcggca atgatcttgg ccgcctacca cccccagcag240
ttcatctacg ccggctcgct gtcggccctg ctggacccct ctcaggggat ggggcctagc300
ctgatcggcc tcgcgatggg tgacgccggc ggttacaagg ccgcagacat gtggggtccc360
tcgagtgacc cggcatggga gcgcaacgac cctacgcagc agatccccaa gctggtcgca420
aacaacaccc ggctatgggt ttattgcggg aacggcaccc cgaacgagtt gggcggtgcc480
aacatacccg ccgagttctt ggagaacttc gttcgtagca gcaacctgaa gttccaggat540
gcgtacaacg ccgcgggcgg gcacaacgcc gtgttcaact tcccgcccaa cggcacgcac600
agctgggagt actggggcgc tcagctcaac gccatgaagg gtgacctgca gagttcgtta660
ggcgccggcg gatccggcag cggtaatgat gccccggtca cgttcagaac atcggaaggt720
ggtgcactgg agtggagctt taacagcagt accggagctg gtgcgctgac acagggaacc780
accacatatg ccatgcacgg gcagcaggga aatgacctga atgctggtaa gaacctgata840
tttcaggggc agaatggtca gattaacctt aaggattcgg tttctcaggg ggcgggttcc900
ctgacgttcc gtgataatta cacagtaaca acctctaacg gaagtacctg gaccggtgcc960
ggtattgttg tggacaacgg ggtgtccgta aactggcagg ttaatggtgt taagggcgat1020
aacctgcata aaattggtga aggtacgctg acggtacagg gtacaggtat taatgaaggt1080
ggcctgaagg tcggggacgg aaaggttgta ctgaaccagc aggcggacaa taaaggacag1140
gtgcaggcgt tcagcagtgt taatattgcc agtggccggc cgaccgtggt actgactgat1200
gagcggcagg taaatccgga taccgtctca tggggatatc gtgggggcac actggatgtt1260
aatggtaaca gtctgacgtt tcatcagttg aaggcggcag attatggtgc cgtgctggcg1320
aataacgttg ataaacgggc cactatcacg ctggactatg ccggttctgg gagctccttc1380
tcccggccgg ggctgccggt cgagtacctg caggtgccgt cgccgtcgat gggccgcgac1440
atcaaggttc agttccagag cggtgggaac aactcacctg cggtttatct gctcgacggc1500
ctgcgcgccc aagacgacta caacggctgg gatatcaaca ccccggcgtt cgagtggtac1560
taccagtcgg gactgtcgat agtcatgccg gtcggcgggc agtccagctt ctacagcgac1620
tggtacagcc cggcctgcgg taaggctggc tgccagactt acaagtggga aaccttcctg1680
accagcgagc tgccgcaatg gttgtccgcc aacagggccg tgaagcccac cggcagcgct1740
gcaatcggct tgtcgggatc cggcagcggt aatactgcag ggtatctgtt tcacggacaa1800
ctgaaaggca atctgaatgt ggacaatcgc ctgcctgaag gcgttaccgg tgctctggtg1860
atggacggag ctgcggatat ctccggtaca ttcacccagg aaaacgggcg tctgacgctg1920
caggggcatc cggttatcca tgcatacaat actcagtctg tggctgacaa actggctgcc1980
agtggagacc attcggttct gactcagcct acgtcattca gtcaggagga ctgggagaac2040
cgcagtttta cctttgacag gctgtcactg aagaacactg attttggtct tggtcgcaat2100
gccacactga acacaaccat ccaggcagat aactccagcg tcacgctggg cgacagccgg2160
gtatttatcg acaaaaacga tggccaggga acagccttta cccttgaaga aggcacatct2220
gttgcaacta aagatgcaga taaaagtgtc ttcaacggca ccgtcaacct ggataatcag2280
tcagtgctga atatcaatga tatattcaat ggcggaatac aggcgaacaa cagtaccgtg2340
aatatctcct cagacagtgc cgttctgggg aactcaacac tgaccagtac cgccctgaat2400
ctgaacaagg gagcaaatgc tctggccagt cagagttttg tttctgacgg tccagtgaat2460
atttctgatg ccaccctgag tctgaacagc cgtcctgatg aggtatctca cacactttta2520
cctgtatacg attatgccgg ttcatggaac ctgaagggag acgatgcccg cctgaacgtg2580
gggccgtaca gtatgttgtc aggtaatatc aatgttcagg ataaagggac tgtcaccctc2640
ggaggggaag gggaactgag tcctgacctg actcttcaga atcagatgtt gtacagcctg2700
tttaacgggt accgcaatat ctggagcggg agcctgaatg caccggatgc caccgtcagc2760
atgacagaca cccagtggtc gatgaacgga aactccacgg caggaaatat gaaacttaac2820
cggacaatag tcggttttaa cgggggaaca tcaccgttca cgacactgac aacagataat2880
ctggacgcgg ttcagtcagc atttgtcatg cgtacagacc ttaacaaggc agacaaactg2940
gtgataaaca agtcggcaac aggtcatgac aacagcatct gggttaactt cctgaaaaaa3000
ccttctaaca aggacacgct tgatattcca ctggtcagcg cacctgaagc gacagctgat3060
aatctgttca gggcatcaac acgggttgtg ggattcagtg atgtcacccc catccttagt3120
gtcagaaaag aggacgggaa aaaagagtgg gtcctcgatg gttaccaggt tgcacgtaac3180
gacggccagg gtaaggctgc cgccacattc atgcacatca gctataacaa cttcatcact3240
gaagttaaca acctgaacaa acgcatgggc gatttgaggg atattaatgg cgaagccggt3300
acgtgggtgc gtctgctgaa cggttccggc tctgctgatg gcggtttcac tgaccactat3360
accctgctgc agatgggggc tgaccgtaag cacgaactgg gaagtatgga cctgtttacc3420
ggcgtgatgg ccacctacac tgacacagat gcgtcagcag acctgtacag cggtaaaaca3480
aaatcatggg gtggtggttt ctatgccagt ggtctgttcc ggtccggcgc ttactttgat3540
gtgattgcca aatatattca caatgaaaac aaatatgacc tgaactttgc cggagctggt3600
aaacagaact tccgcagcca ttcactgtat gcaggtgcag aagtcggata ccgttatcat3660
ctgacagata cgacgtttgt tgaacctcag gcggaactgg tctggggaag actgcagggc3720
caaacattta actggaacga cagtggaatg gatgtctcaa tgcgtcgtaa cagcgttaat3780
cctctggtag gcagaaccgg cgttgtttcc ggtaaaacct tcagtggtaa ggactggagt3840
ctgacagccc gtgccggcct gcattatgag ttcgatctga cggacagtgc tgacgttcat3900
ctgaaggatg cagcgggaga acatcagatt aatggcagaa aagacagtcg tatgctttac3960
ggtgtggggt taaatgcccg gtttggcgac aatacgcgtc tggggctgga agttgaacgc4020
tctgcatttg gtaaatacaa cacagatgat gcgataaacg ctaatattcg ttattcattc4080
tga 4083
<210>78
<211>4083
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>78
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccaccgg cagcgctgca180
atcggcttgt cgatggccgg ctcgtcggca atgatcttgg ccgcctacca cccccagcag240
ttcatctacg ccggctcgct gtcggccctg ctggacccct ctcaggggat ggggcctagc300
ctgatcggcc tcgcgatggg tgacgccggc ggttacaagg ccgcagacat gtggggtccc360
tcgagtgacc cggcatggga gcgcaacgac cctacgcagc agatccccaa gctggtcgca420
aacaacaccc ggctatgggt ttattgcggg aacggcaccc cgaacgagtt gggcggtgcc480
aacatacccg ccgagttctt ggagaacttc gttcgtagca gcaacctgaa gttccaggat540
gcgtacaacg ccgcgggcgg gcacaacgcc gtgttcaact tcccgcccaa cggcacgcac600
agctgggagt actggggcgc tcagctcaac gccatgaagg gtgacctgca gagttcgtta660
ggcgccggcg gatccggcag cggtaatgat gccccggtca cgttcagaac atcggaaggt720
ggtgcactgg agtggagctt taacagcagt accggagctg gtgcgctgac acagggaacc780
accacatatg ccatgcacgg gcagcaggga aatgacctga atgctggtaa gaacctgata840
tttcaggggc agaatggtca gattaacctt aaggattcgg tttctcaggg ggcgggttcc900
ctgacgttcc gtgataatta cacagtaaca acctctaacg gaagtacctg gaccggtgcc960
ggtattgttg tggacaacgg ggtgtccgta aactggcagg ttaatggtgt taagggcgat1020
aacctgcata aaattggtga aggtacgctg acggtacagg gtacaggtat taatgaaggt1080
ggcctgaagg tcggggacgg aaaggttgta ctgaaccagc aggcggacaa taaaggacag1140
gtgcaggcgt tcagcagtgt taatattgcc agtggccggc cgaccgtggt actgactgat1200
gagcggcagg taaatccgga taccgtctca tggggatatc gtgggggcac actggatgtt1260
aatggtaaca gtctgacgtt tcatcagttg aaggcggcag attatggtgc cgtgctggcg1320
aataacgttg ataaacgggc cactatcacg ctggactatg ccggttctgg gagctccttc1380
tcccggccgg ggctgccggt cgagtacctg caggtgccgt cgccgtcgat gggccgcgac1440
atcaaggttc agttccagag cggtgggaac aactcacctg cggtttatct gctcgacggc1500
ctgcgcgccc aagacgacta caacggctgg gatatcaaca ccccggcgtt cgagtggtac1560
taccagtcgg gactgtcgat agtcatgccg gtcggcgggc agtccagctt ctacagcgac1620
tggtacagcc cggcctgcgg taaggctggc tgccagactt acaagtggga aaccttcctg1680
accagcgagc tgccgcaatg gttgtccgcc aacagggccg tgaagcccac cggcagcgct1740
gcaatcggct tgtcgggatc cggcagcggt aatactgcag ggtatctgtt tcacggacaa1800
ctgaaaggca atctgaatgt ggacaatcgc ctgcctgaag gcgttaccgg tgctctggtg1860
atggacggag ctgcggatat ctccggtaca ttcacccagg aaaacgggcg tctgacgctg1920
caggggcatc cggttatcca tgcatacaat actcagtctg tggctgacaa actggctgcc1980
agtggagacc attcggttct gactcagcct acgtcattca gtcaggagga ctgggagaac2040
cgcagtttta cctttgacag gctgtcactg aagaacactg attttggtct tggtcgcaat2100
gccacactga acacaaccat ccaggcagat aactccagcg tcacgctggg cgacagccgg2160
gtatttatcg acaaaaacga tggccaggga acagccttta cccttgaaga aggcacatct2220
gttgcaacta aagatgcaga taaaagtgtc ttcaacggca ccgtcaacct ggataatcag2280
tcagtgctga atatcaatga tatattcaat ggcggaatac aggcgaacaa cagtaccgtg2340
aatatctcct cagacagtgc cgttctgggg aactcaacac tgaccagtac cgccctgaat2400
ctgaacaagg gagcaaatgc tctggccagt cagagttttg tttctgacgg tccagtgaat2460
atttctgatg ccaccctgag tctgaacagc cgtcctgatg aggtatctca cacactttta2520
cctgtatacg attatgccgg ttcatggaac ctgaagggag acgatgcccg cctgaacgtg2580
gggccgtaca gtatgttgtc aggtaatatc aatgttcagg ataaagggac tgtcaccctc2640
ggaggggaag gggaactgag tcctgacctg actcttcaga atcagatgtt gtacagcctg2700
tttaacgggt accgcaatat ctggagcggg agcctgaatg caccggatgc caccgtcagc2760
atgacagaca cccagtggtc gatgaacgga aactccacgg caggaaatat gaaacttaac2820
cggacaatag tcggttttaa cgggggaaca tcaccgttca cgacactgac aacagataat2880
ctggacgcgg ttcagtcagc atttgtcatg cgtacagacc ttaacaaggc agacaaactg2940
gtgataaaca agtcggcaac aggtcatgac aacagcatct gggttaactt cctgaaaaaa3000
ccttctaaca aggacacgct tgatattcca ctggtcagcg cacctgaagc gacagctgat3060
aatctgttca gggcatcaac acgggttgtg ggattcagtg atgtcacccc catccttagt3120
gtcagaaaag aggacgggaa aaaagagtgg gtcctcgatg gttaccaggt tgcacgtaac3180
gacggccagg gtaaggctgc cgccacattc atgcacatca gctataacaa cttcatcact3240
gaagttggtt ccctgaacaa acgcatgggc gatttgaggg atattaatgg cgaagccggt3300
acgtgggtgc gtctgctgaa cggttccggc tctgctgatg gcggtttcac tgaccactat3360
accctgctgc agatgggggc tgaccgtaag cacgaactgg gaagtatgga cctgtttacc3420
ggcgtgatgg ccacctacac tgacacagat gcgtcagcag acctgtacag cggtaaaaca3480
aaatcatggg gtggtggttt ctatgccagt ggtctgttcc ggtccggcgc ttactttgat3540
gtgattgcca aatatattca caatgaaaac aaatatgacc tgaactttgc cggagctggt3600
aaacagaact tccgcagcca ttcactgtat gcaggtgcag aagtcggata ccgttatcat3660
ctgacagata cgacgtttgt tgaacctcag gcggaactgg tctggggaag actgcagggc3720
caaacattta actggaacga cagtggaatg gatgtctcaa tgcgtcgtaa cagcgttaat3780
cctctggtag gcagaaccgg cgttgtttcc ggtaaaacct tcagtggtaa ggactggagt3840
ctgacagccc gtgccggcct gcattatgag ttcgatctga cggacagtgc tgacgttcat3900
ctgaaggatg cagcgggaga acatcagatt aatggcagaa aagacagtcg tatgctttac3960
ggtgtggggt taaatgcccg gtttggcgac aatacgcgtc tggggctgga agttgaacgc4020
tctgcatttg gtaaatacaa cacagatgat gcgataaacg ctaatattcg ttattcattc4080
tga4083
<210>79
<211>4383
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>79
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccaccgg cagcgctgca180
atcggcttgt cgatggccgg ctcgtcggca atgatcttgg ccgcctacca cccccagcag240
ttcatctacg ccggctcgct gtcggccctg ctggacccct ctcaggggat ggggcctagc300
ctgatcggcc tcgcgatggg tgacgccggc ggttacaagg ccgcagacat gtggggtccc360
tcgagtgacc cggcatggga gcgcaacgac cctacgcagc agatccccaa gctggtcgca420
aacaacaccc ggctatgggt ttattgcggg aacggcaccc cgaacgagtt gggcggtgcc480
aacatacccg ccgagttctt ggagaacttc gttcgtagca gcaacctgaa gttccaggat540
gcgtacaacg ccgcgggcgg gcacaacgcc gtgttcaact tcccgcccaa cggcacgcac600
agctgggagt actggggcgc tcagctcaac gccatgaagg gtgacctgca gagttcgtta660
ggcgccggcg gatccggcag cggtaatgat gccccggtca cgttcagaac atcggaaggt720
ggtgcactgg agtggagctt taacagcagt accggagctg gtgcgctgac acagggaacc780
accacatatg ccatgcacgg gcagcaggga aatgacctga atgctggtaa gaacctgata840
tttcaggggc agaatggtca gattaacctt aaggattcgg tttctcaggg ggcgggttcc900
ctgacgttcc gtgataatta cacagtaaca acctctaacg gaagtacctg gaccggtgcc960
ggtattgttg tggacaacgg ggtgtccgta aactggcagg ttaatggtgt taagggcgat1020
aacctgcata aaattggtga aggtacgctg acggtacagg gtacaggtat taatgaaggt1080
ggcctgaagg tcggggacgg aaaggttgta ctgaaccagc aggcggacaa taaaggacag1140
gtgcaggcgt tcagcagtgt taatattgcc agtggccggc cgaccgtggt actgactgat1200
gagcggcagg taaatccgga taccgtctca tggggatatc gtgggggcac actggatgtt1260
aatggtaaca gtctgacgtt tcatcagttg aaggcggcag attatggtgc cgtgctggcg1320
aataacgttg ataaacgggc cactatcacg ctggactatg ccggttctgg gagctccttc1380
tcccggccgg ggctgccggt cgagtacctg caggtgccgt cgccgtcgat gggccgcgac1440
atcaaggttc agttccagag cggtgggaac aactcacctg cggtttatct gctcgacggc1500
ctgcgcgccc aagacgacta caacggctgg gatatcaaca ccccggcgtt cgagtggtac1560
taccagtcgg gactgtcgat agtcatgccg gtcggcgggc agtccagctt ctacagcgac1620
tggtacagcc cggcctgcgg taaggctggc tgccagactt acaagtggga aaccttcctg1680
accagcgagc tgccgcaatg gttgtccgcc aacagggccg tgaagcccac cggcagcgct1740
gcaatcggct tgtcgggatc cggcagcggt aatactgcag ggtatctgtt tcacggacaa1800
ctgaaaggca atctgaatgt ggacaatcgc ctgcctgaag gcgttaccgg tgctctggtg1860
atggacggag ctgcggatat ctccggtaca ttcacccagg aaaacgggcg tctgacgctg1920
caggggcatc cggttatcca tgcatacaat actcagtctg tggctgacaa actggctgcc1980
agtggagacc attcggttct gactcagcct acgtcattca gtcaggagga ctgggagaac2040
cgcagtttta cctttgacag gctgtcactg aagaacactg attttggtct tggtcgcaat2100
gccacactga acacaaccat ccaggcagat aactccagcg tcacgctggg cgacagccgg2160
gtatttatcg acaaaaacga tggccaggga acagccttta cccttgaaga aggcacatct2220
gttgcaggtt ctgggagctc catgaccgaa cagcagtgga acttcgcggg tatcgaagcg2280
gcggcgagcg cgatccaggg taacgtgacc agcatccaca gcctgctgga tgaaggtaaa2340
cagagcctga ccaaactggc ggcggcctgg ggtggtagcg gtagcgaagc gtatcagggt2400
gtgcagcaga aatgggatgc gaccgcgacc gaactgaaca acgcgctgca gaacctggcg2460
cgtaccatca gcgaagcggg tcaggcgatg gcgagcaccg aaggtaacgt gaccggtatg2520
ttcgcgggat ccggcagcgg taaaagtgtc ttcaacggca ccgtcaacct ggataatcag2580
tcagtgctga atatcaatga tatattcaat ggcggaatac aggcgaacaa cagtaccgtg2640
aatatctcct cagacagtgc cgttctgggg aactcaacac tgaccagtac cgccctgaat2700
ctgaacaagg gagcaaatgc tctggccagt cagagttttg tttctgacgg tccagtgaat2760
atttctgatg ccaccctgag tctgaacagc cgtcctgatg aggtatctca cacactttta2820
cctgtatacg attatgccgg ttcatggaac ctgaagggag acgatgcccg cctgaacgtg2880
gggccgtaca gtatgttgtc aggtaatatc aatgttcagg ataaagggac tgtcaccctc2940
ggaggggaag gggaactgag tcctgacctg actcttcaga atcagatgtt gtacagcctg3000
tttaacgggt accgcaatat ctggagcggg agcctgaatg caccggatgc caccgtcagc3060
atgacagaca cccagtggtc gatgaacgga aactccacgg caggaaatat gaaacttaac3120
cggacaatag tcggttttaa cgggggaaca tcaccgttca cgacactgac aacagataat3180
ctggacgcgg ttcagtcagc atttgtcatg cgtacagacc ttaacaaggc agacaaactg3240
gtgataaaca agtcggcaac aggtcatgac aacagcatct gggttaactt cctgaaaaaa3300
ccttctaaca aggacacgct tgatattcca ctggtcagcg cacctgaagc gacagctgat3360
aatctgttca gggcatcaac acgggttgtg ggattcagtg atgtcacccc catccttagt3420
gtcagaaaag aggacgggaa aaaagagtgg gtcctcgatg gttaccaggt tgcacgtaac3480
gacggccagg gtaaggctgc cgccacattc atgcacatca gctataacaa cttcatcact3540
gaagttaaca acctgaacaa acgcatgggc gatttgaggg atattaatgg cgaagccggt3600
acgtgggtgc gtctgctgaa cggttccggc tctgctgatg gcggtttcac tgaccactat3660
accctgctgc agatgggggc tgaccgtaag cacgaactgg gaagtatgga cctgtttacc3720
ggcgtgatgg ccacctacac tgacacagat gcgtcagcag acctgtacag cggtaaaaca3780
aaatcatggg gtggtggttt ctatgccagt ggtctgttcc ggtccggcgc ttactttgat3840
gtgattgcca aatatattca caatgaaaac aaatatgacc tgaactttgc cggagctggt3900
aaacagaact tccgcagcca ttcactgtat gcaggtgcag aagtcggata ccgttatcat3960
ctgacagata cgacgtttgt tgaacctcag gcggaactgg tctggggaag actgcagggc4020
caaacattta actggaacga cagtggaatg gatgtctcaa tgcgtcgtaa cagcgttaat4080
cctctggtag gcagaaccgg cgttgtttcc ggtaaaacct tcagtggtaa ggactggagt4140
ctgacagccc gtgccggcct gcattatgag ttcgatctga cggacagtgc tgacgttcat4200
ctgaaggatg cagcgggaga acatcagatt aatggcagaa aagacagtcg tatgctttac4260
ggtgtggggt taaatgcccg gtttggcgac aatacgcgtc tggggctgga agttgaacgc4320
tctgcatttg gtaaatacaa cacagatgat gcgataaacg ctaatattcg ttattcattc4380
tga 4383
<210>80
<211>4383
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>80
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccaccgg cagcgctgca180
atcggcttgt cgatggccgg ctcgtcggca atgatcttgg ccgcctacca cccccagcag240
ttcatctacg ccggctcgct gtcggccctg ctggacccct ctcaggggat ggggcctagc300
ctgatcggcc tcgcgatggg tgacgccggc ggttacaagg ccgcagacat gtggggtccc360
tcgagtgacc cggcatggga gcgcaacgac cctacgcagc agatccccaa gctggtcgca420
aacaacaccc ggctatgggt ttattgcggg aacggcaccc cgaacgagtt gggcggtgcc480
aacatacccg ccgagttctt ggagaacttc gttcgtagca gcaacctgaa gttccaggat540
gcgtacaacg ccgcgggcgg gcacaacgcc gtgttcaact tcccgcccaa cggcacgcac600
agctgggagt actggggcgc tcagctcaac gccatgaagg gtgacctgca gagttcgtta660
ggcgccggcg gatccggcag cggtaatgat gccccggtca cgttcagaac atcggaaggt720
ggtgcactgg agtggagctt taacagcagt accggagctg gtgcgctgac acagggaacc780
accacatatg ccatgcacgg gcagcaggga aatgacctga atgctggtaa gaacctgata840
tttcaggggc agaatggtca gattaacctt aaggattcgg tttctcaggg ggcgggttcc900
ctgacgttcc gtgataatta cacagtaaca acctctaacg gaagtacctg gaccggtgcc960
ggtattgttg tggacaacgg ggtgtccgta aactggcagg ttaatggtgt taagggcgat1020
aacctgcata aaattggtga aggtacgctg acggtacagg gtacaggtat taatgaaggt1080
ggcctgaagg tcggggacgg aaaggttgta ctgaaccagc aggcggacaa taaaggacag1140
gtgcaggcgt tcagcagtgt taatattgcc agtggccggc cgaccgtggt actgactgat1200
gagcggcagg taaatccgga taccgtctca tggggatatc gtgggggcac actggatgtt1260
aatggtaaca gtctgacgtt tcatcagttg aaggcggcag attatggtgc cgtgctggcg1320
aataacgttg ataaacgggc cactatcacg ctggactatg ccggttctgg gagctccttc1380
tcccggccgg ggctgccggt cgagtacctg caggtgccgt cgccgtcgat gggccgcgac1440
atcaaggttc agttccagag cggtgggaac aactcacctg cggtttatct gctcgacggc1500
ctgcgcgccc aagacgacta caacggctgg gatatcaaca ccccggcgtt cgagtggtac1560
taccagtcgg gactgtcgat agtcatgccg gtcggcgggc agtccagctt ctacagcgac1620
tggtacagcc cggcctgcgg taaggctggc tgccagactt acaagtggga aaccttcctg1680
accagcgagc tgccgcaatg gttgtccgcc aacagggccg tgaagcccac cggcagcgct1740
gcaatcggct tgtcgggatc cggcagcggt aatactgcag ggtatctgtt tcacggacaa1800
ctgaaaggca atctgaatgt ggacaatcgc ctgcctgaag gcgttaccgg tgctctggtg1860
atggacggag ctgcggatat ctccggtaca ttcacccagg aaaacgggcg tctgacgctg1920
caggggcatc cggttatcca tgcatacaat actcagtctg tggctgacaa actggctgcc1980
agtggagacc attcggttct gactcagcct acgtcattca gtcaggagga ctgggagaac2040
cgcagtttta cctttgacag gctgtcactg aagaacactg attttggtct tggtcgcaat2100
gccacactga acacaaccat ccaggcagat aactccagcg tcacgctggg cgacagccgg2160
gtatttatcg acaaaaacga tggccaggga acagccttta cccttgaaga aggcacatct2220
gttgcaggtt ctgggagctc catgaccgaa cagcagtgga acttcgcggg tatcgaagcg2280
gcggcgagcg cgatccaggg taacgtgacc agcatccaca gcctgctgga tgaaggtaaa2340
cagagcctga ccaaactggc ggcggcctgg ggtggtagcg gtagcgaagc gtatcagggt2400
gtgcagcaga aatgggatgc gaccgcgacc gaactgaaca acgcgctgca gaacctggcg2460
cgtaccatca gcgaagcggg tcaggcgatg gcgagcaccg aaggtaacgt gaccggtatg2520
ttcgcgggat ccggcagcgg taaaagtgtc ttcaacggca ccgtcaacct ggataatcag2580
tcagtgctga atatcaatga tatattcaat ggcggaatac aggcgaacaa cagtaccgtg2640
aatatctcct cagacagtgc cgttctgggg aactcaacac tgaccagtac cgccctgaat2700
ctgaacaagg gagcaaatgc tctggccagt cagagttttg tttctgacgg tccagtgaat2760
atttctgatg ccaccctgag tctgaacagc cgtcctgatg aggtatctca cacactttta2820
cctgtatacg attatgccgg ttcatggaac ctgaagggag acgatgcccg cctgaacgtg2880
gggccgtaca gtatgttgtc aggtaatatc aatgttcagg ataaagggac tgtcaccctc2940
ggaggggaag gggaactgag tcctgacctg actcttcaga atcagatgtt gtacagcctg3000
tttaacgggt accgcaatat ctggagcggg agcctgaatg caccggatgc caccgtcagc3060
atgacagaca cccagtggtc gatgaacgga aactccacgg caggaaatat gaaacttaac3120
cggacaatag tcggttttaa cgggggaaca tcaccgttca cgacactgac aacagataat3180
ctggacgcgg ttcagtcagc atttgtcatg cgtacagacc ttaacaaggc agacaaactg3240
gtgataaaca agtcggcaac aggtcatgac aacagcatct gggttaactt cctgaaaaaa3300
ccttctaaca aggacacgct tgatattcca ctggtcagcg cacctgaagc gacagctgat3360
aatctgttca gggcatcaac acgggttgtg ggattcagtg atgtcacccc catccttagt3420
gtcagaaaag aggacgggaa aaaagagtgg gtcctcgatg gttaccaggt tgcacgtaac3480
gacggccagg gtaaggctgc cgccacattc atgcacatca gctataacaa cttcatcact3540
gaagttggtt ccctgaacaa acgcatgggc gatttgaggg atattaatgg cgaagccggt3600
acgtgggtgc gtctgctgaa cggttccggc tctgctgatg gcggtttcac tgaccactat3660
accctgctgc agatgggggc tgaccgtaag cacgaactgg gaagtatgga cctgtttacc3720
ggcgtgatgg ccacctacac tgacacagat gcgtcagcag acctgtacag cggtaaaaca3780
aaatcatggg gtggtggttt ctatgccagt ggtctgttcc ggtccggcgc ttactttgat3840
gtgattgcca aatatattca caatgaaaac aaatatgacc tgaactttgc cggagctggt3900
aaacagaact tccgcagcca ttcactgtat gcaggtgcag aagtcggata ccgttatcat3960
ctgacagata cgacgtttgt tgaacctcag gcggaactgg tctggggaag actgcagggc4020
caaacattta actggaacga cagtggaatg gatgtctcaa tgcgtcgtaa cagcgttaat4080
cctctggtag gcagaaccgg cgttgtttcc ggtaaaacct tcagtggtaa ggactggagt4140
ctgacagccc gtgccggcct gcattatgag ttcgatctga cggacagtgc tgacgttcat4200
ctgaaggatg cagcgggaga acatcagatt aatggcagaa aagacagtcg tatgctttac4260
ggtgtggggt taaatgcccg gtttggcgac aatacgcgtc tggggctgga agttgaacgc4320
tctgcatttg gtaaatacaa cacagatgat gcgataaacg ctaatattcg ttattcattc4380
tga 4383
<210>81
<211>4572
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>81
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccaccgg cagcgctgca180
atcggcttgt cgatggccgg ctcgtcggca atgatcttgg ccgcctacca cccccagcag240
ttcatctacg ccggctcgct gtcggccctg ctggacccct ctcaggggat ggggcctagc300
ctgatcggcc tcgcgatggg tgacgccggc ggttacaagg ccgcagacat gtggggtccc360
tcgagtgacc cggcatggga gcgcaacgac cctacgcagc agatccccaa gctggtcgca420
aacaacaccc ggctatgggt ttattgcggg aacggcaccc cgaacgagtt gggcggtgcc480
aacatacccg ccgagttctt ggagaacttc gttcgtagca gcaacctgaa gttccaggat540
gcgtacaacg ccgcgggcgg gcacaacgcc gtgttcaact tcccgcccaa cggcacgcac600
agctgggagt actggggcgc tcagctcaac gccatgaagg gtgacctgca gagttcgtta660
ggcgccggcg gatccggcag cggtaatgat gccccggtca cgttcagaac atcggaaggt720
ggtgcactgg agtggagctt taacagcagt accggagctg gtgcgctgac acagggaacc780
accacatatg ccatgcacgg gcagcaggga aatgacctga atgctggtaa gaacctgata840
tttcaggggc agaatggtca gattaacctt aaggattcgg tttctcaggg ggcgggttcc900
ctgacgttcc gtgataatta cacagtaaca acctctaacg gaagtacctg gaccggtgcc960
ggtattgttg tggacaacgg ggtgtccgta aactggcagg ttaatggtgt taagggcgat1020
aacctgcata aaattggtga aggtacgctg acggtacagg gtacaggtat taatgaaggt1080
ggcctgaagg tcggggacgg aaaggttgta ctgaaccagc aggcggacaa taaaggacag1140
gtgcaggcgt tcagcagtgt taatattgcc agtggccggc cgaccgtggt actgactgat1200
gagcggcagg taaatccgga taccgtctca tggggatatc gtgggggcac actggatgtt1260
aatggtaaca gtctgacgtt tcatcagttg aaggcggcag attatggtgc cgtgctggcg1320
aataacgttg ataaacgggc cactatcacg ctggactatg ccggttctgg gagctccttc1380
tcccggccgg ggctgccggt cgagtacctg caggtgccgt cgccgtcgat gggccgcgac1440
atcaaggttc agttccagag cggtgggaac aactcacctg cggtttatct gctcgacggc1500
ctgcgcgccc aagacgacta caacggctgg gatatcaaca ccccggcgtt cgagtggtac1560
taccagtcgg gactgtcgat agtcatgccg gtcggcgggc agtccagctt ctacagcgac1620
tggtacagcc cggcctgcgg taaggctggc tgccagactt acaagtggga aaccttcctg1680
accagcgagc tgccgcaatg gttgtccgcc aacagggccg tgaagcccac cggcagcgct1740
gcaatcggct tgtcgggatc cggcagcggt aatactgcag ggtatctgtt tcacggacaa1800
ctgaaaggca atctgaatgt ggacaatcgc ctgcctgaag gcgttaccgg tgctctggtg1860
atggacggag ctgcggatat ctccggtaca ttcacccagg aaaacgggcg tctgacgctg1920
caggggcatc cggttatcca tgcatacaat actcagtctg tggctgacaa actggctgcc1980
agtggagacc attcggttct gactcagcct acgtcattca gtcaggagga ctgggagaac2040
cgcagtttta cctttgacag gctgtcactg aagaacactg attttggtct tggtcgcaat2100
gccacactga acacaaccat ccaggcagat aactccagcg tcacgctggg cgacagccgg2160
gtatttatcg acaaaaacga tggccaggga acagccttta cccttgaaga aggcacatct2220
gttgcaggtt ctgggagctc catgaccgaa cagcagtgga acttcgcggg tatcgaagcg2280
gcggcgagcg cgatccaggg taacgtgacc agcatccaca gcctgctgga tgaaggtaaa2340
cagagcctga ccaaactggc ggcggcctgg ggtggtagcg gtagcgaagc gtatcagggt2400
gtgcagcaga aatgggatgc gaccgcgacc gaactgaaca acgcgctgca gaacctggcg2460
cgtaccatca gcgaagcggg tcaggcgatg gcgagcaccg aaggtaacgt gaccggtatg2520
ttcgcgggat ccggcagcgg taaaagtgtc ttcaacggca ccgtcaacct ggataatcag2580
tcagtgctga atatcaatga tatattcaat ggcggaatac aggcgaacaa cagtaccgtg2640
aatatctcct cagacagtgc cgttctgggg aactcaacac tgaccagtac cgccctgaat2700
ctgaacaagg gagcaaatgc tctggccagt cagagttttg tttctgacgg tccagtgaat2760
atttctgatg ccaccctgag tctgaacagc cgtcctgatg aggtatctca cacactttta2820
cctgtatacg attatgccgg ttcatggaac ctgaagggag acgatgcccg cctgaacgtg2880
gggccgtaca gtatgttgtc aggtaatatc aatgttcagg ataaagggac tgtcaccctc2940
ggagggagcg ggagctccgt gatagcgggc gtcgaccagg cgcttgcagc aacaggccag3000
gctagccagc gggcggcagg cgcatctggt ggggtcaccg tcggtgtcgg cgtgggcacg3060
gaacagagga acctttcggt ggttgcaccg agtcagttca catttagttc acgcagccca3120
gattttgtgg atgaaaccgc aggtcaatcg tggtgcgcga tactgggatt gaaccagttt3180
cacggatccg gcagcgggta ccgcaatatc tggagcggga gcctgaatgc accggatgcc3240
accgtcagca tgacagacac ccagtggtcg atgaacggaa actccacggc aggaaatatg3300
aaacttaacc ggacaatagt cggttttaac gggggaacat caccgttcac gacactgaca3360
acagataatc tggacgcggt tcagtcagca tttgtcatgc gtacagacct taacaaggca3420
gacaaactgg tgataaacaa gtcggcaaca ggtcatgaca acagcatctg ggttaacttc3480
ctgaaaaaac cttctaacaa ggacacgctt gatattccac tggtcagcgc acctgaagcg3540
acagctgata atctgttcag ggcatcaaca cgggttgtgg gattcagtga tgtcaccccc3600
atccttagtg tcagaaaaga ggacgggaaa aaagagtggg tcctcgatgg ttaccaggtt3660
gcacgtaacg acggccaggg taaggctgcc gccacattca tgcacatcag ctataacaac3720
ttcatcactg aagttaacaa cctgaacaaa cgcatgggcg atttgaggga tattaatggc3780
gaagccggta cgtgggtgcg tctgctgaac ggttccggct ctgctgatgg cggtttcact3840
gaccactata ccctgctgca gatgggggct gaccgtaagc acgaactggg aagtatggac3900
ctgtttaccg gcgtgatggc cacctacact gacacagatg cgtcagcaga cctgtacagc3960
ggtaaaacaa aatcatgggg tggtggtttc tatgccagtg gtctgttccg gtccggcgct4020
tactttgatg tgattgccaa atatattcac aatgaaaaca aatatgacct gaactttgcc4080
ggagctggta aacagaactt ccgcagccat tcactgtatg caggtgcaga agtcggatac4140
cgttatcatc tgacagatac gacgtttgtt gaacctcagg cggaactggt ctggggaaga4200
ctgcagggcc aaacatttaa ctggaacgac agtggaatgg atgtctcaat gcgtcgtaac4260
agcgttaatc ctctggtagg cagaaccggc gttgtttccg gtaaaacctt cagtggtaag4320
gactggagtc tgacagcccg tgccggcctg cattatgagt tcgatctgac ggacagtgct4380
gacgttcatc tgaaggatgc agcgggagaa catcagatta atggcagaaa agacagtcgt4440
atgctttacg gtgtggggtt aaatgcccgg tttggcgaca atacgcgtct ggggctggaa4500
gttgaacgct ctgcatttgg taaatacaac acagatgatg cgataaacgc taatattcgt4560
tattcattct ga 4572
<210>82
<211>4572
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>82
atgaacagaa tttattctct tcgctacagc gctgtggccc ggggctttat tgccgtatct60
gagtttgcta ggaaatgtgt tcataagtct gtcagacgtc tgtgtttccc ggttttatta120
ctgatcccgg tactattctc tgcaggaagt cttgcgggga gctccaccgg cagcgctgca180
atcggcttgt cgatggccgg ctcgtcggca atgatcttgg ccgcctacca cccccagcag240
ttcatctacg ccggctcgct gtcggccctg ctggacccct ctcaggggat ggggcctagc300
ctgatcggcc tcgcgatggg tgacgccggc ggttacaagg ccgcagacat gtggggtccc360
tcgagtgacc cggcatggga gcgcaacgac cctacgcagc agatccccaa gctggtcgca420
aacaacaccc ggctatgggt ttattgcggg aacggcaccc cgaacgagtt gggcggtgcc480
aacatacccg ccgagttctt ggagaacttc gttcgtagca gcaacctgaa gttccaggat540
gcgtacaacg ccgcgggcgg gcacaacgcc gtgttcaact tcccgcccaa cggcacgcac600
agctgggagt actggggcgc tcagctcaac gccatgaagg gtgacctgca gagttcgtta660
ggcgccggcg gatccggcag cggtaatgat gccccggtca cgttcagaac atcggaaggt720
ggtgcactgg agtggagctt taacagcagt accggagctg gtgcgctgac acagggaacc780
accacatatg ccatgcacgg gcagcaggga aatgacctga atgctggtaa gaacctgata840
tttcaggggc agaatggtca gattaacctt aaggattcgg tttctcaggg ggcgggttcc900
ctgacgttcc gtgataatta cacagtaaca acctctaacg gaagtacctg gaccggtgcc960
ggtattgttg tggacaacgg ggtgtccgta aactggcagg ttaatggtgt taagggcgat1020
aacctgcata aaattggtga aggtacgctg acggtacagg gtacaggtat taatgaaggt1080
ggcctgaagg tcggggacgg aaaggttgta ctgaaccagc aggcggacaa taaaggacag1140
gtgcaggcgt tcagcagtgt taatattgcc agtggccggc cgaccgtggt actgactgat1200
gagcggcagg taaatccgga taccgtctca tggggatatc gtgggggcac actggatgtt1260
aatggtaaca gtctgacgtt tcatcagttg aaggcggcag attatggtgc cgtgctggcg1320
aataacgttg ataaacgggc cactatcacg ctggactatg ccggttctgg gagctccttc1380
tcccggccgg ggctgccggt cgagtacctg caggtgccgt cgccgtcgat gggccgcgac1440
atcaaggttc agttccagag cggtgggaac aactcacctg cggtttatct gctcgacggc1500
ctgcgcgccc aagacgacta caacggctgg gatatcaaca ccccggcgtt cgagtggtac1560
taccagtcgg gactgtcgat agtcatgccg gtcggcgggc agtccagctt ctacagcgac1620
tggtacagcc cggcctgcgg taaggctggc tgccagactt acaagtggga aaccttcctg1680
accagcgagc tgccgcaatg gttgtccgcc aacagggccg tgaagcccac cggcagcgct1740
gcaatcggct tgtcgggatc cggcagcggt aatactgcag ggtatctgtt tcacggacaa1800
ctgaaaggca atctgaatgt ggacaatcgc ctgcctgaag gcgttaccgg tgctctggtg1860
atggacggag ctgcggatat ctccggtaca ttcacccagg aaaacgggcg tctgacgctg1920
caggggcatc cggttatcca tgcatacaat actcagtctg tggctgacaa actggctgcc1980
agtggagacc attcggttct gactcagcct acgtcattca gtcaggagga ctgggagaac2040
cgcagtttta cctttgacag gctgtcactg aagaacactg attttggtct tggtcgcaat2100
gccacactga acacaaccat ccaggcagat aactccagcg tcacgctggg cgacagccgg2160
gtatttatcg acaaaaacga tggccaggga acagccttta cccttgaaga aggcacatct2220
gttgcaggtt ctgggagctc catgaccgaa cagcagtgga acttcgcggg tatcgaagcg2280
gcggcgagcg cgatccaggg taacgtgacc agcatccaca gcctgctgga tgaaggtaaa2340
cagagcctga ccaaactggc ggcggcctgg ggtggtagcg gtagcgaagc gtatcagggt2400
gtgcagcaga aatgggatgc gaccgcgacc gaactgaaca acgcgctgca gaacctggcg2460
cgtaccatca gcgaagcggg tcaggcgatg gcgagcaccg aaggtaacgt gaccggtatg2520
ttcgcgggat ccggcagcgg taaaagtgtc ttcaacggca ccgtcaacct ggataatcag2580
tcagtgctga atatcaatga tatattcaat ggcggaatac aggcgaacaa cagtaccgtg2640
aatatctcct cagacagtgc cgttctgggg aactcaacac tgaccagtac cgccctgaat2700
ctgaacaagg gagcaaatgc tctggccagt cagagttttg tttctgacgg tccagtgaat2760
atttctgatg ccaccctgag tctgaacagc cgtcctgatg aggtatctca cacactttta2820
cctgtatacg attatgccgg ttcatggaac ctgaagggag acgatgcccg cctgaacgtg2880
gggccgtaca gtatgttgtc aggtaatatc aatgttcagg ataaagggac tgtcaccctc2940
ggagggagcg ggagctccgt gatagcgggc gtcgaccagg cgcttgcagc aacaggccag3000
gctagccagc gggcggcagg cgcatctggt ggggtcaccg tcggtgtcgg cgtgggcacg3060
gaacagagga acctttcggt ggttgcaccg agtcagttca catttagttc acgcagccca3120
gattttgtgg atgaaaccgc aggtcaatcg tggtgcgcga tactgggatt gaaccagttt3180
cacggatccg gcagcgggta ccgcaatatc tggagcggga gcctgaatgc accggatgcc3240
accgtcagca tgacagacac ccagtggtcg atgaacggaa actccacggc aggaaatatg3300
aaacttaacc ggacaatagt cggttttaac gggggaacat caccgttcac gacactgaca3360
acagataatc tggacgcggt tcagtcagca tttgtcatgc gtacagacct taacaaggca3420
gacaaactgg tgataaacaa gtcggcaaca ggtcatgaca acagcatctg ggttaacttc3480
ctgaaaaaac cttctaacaa ggacacgctt gatattccac tggtcagcgc acctgaagcg3540
acagctgata atctgttcag ggcatcaaca cgggttgtgg gattcagtga tgtcaccccc3600
atccttagtg tcagaaaaga ggacgggaaa aaagagtggg tcctcgatgg ttaccaggtt3660
gcacgtaacg acggccaggg taaggctgcc gccacattca tgcacatcag ctataacaac3720
ttcatcactg aagttggttc cctgaacaaa cgcatgggcg atttgaggga tattaatggc3780
gaagccggta cgtgggtgcg tctgctgaac ggttccggct ctgctgatgg cggtttcact3840
gaccactata ccctgctgca gatgggggct gaccgtaagc acgaactggg aagtatggac3900
ctgtttaccg gcgtgatggc cacctacact gacacagatg cgtcagcaga cctgtacagc3960
ggtaaaacaa aatcatgggg tggtggtttc tatgccagtg gtctgttccg gtccggcgct4020
tactttgatg tgattgccaa atatattcac aatgaaaaca aatatgacct gaactttgcc4080
ggagctggta aacagaactt ccgcagccat tcactgtatg caggtgcaga agtcggatac4140
cgttatcatc tgacagatac gacgtttgtt gaacctcagg cggaactggt ctggggaaga4200
ctgcagggcc aaacatttaa ctggaacgac agtggaatgg atgtctcaat gcgtcgtaac4260
agcgttaatc ctctggtagg cagaaccggc gttgtttccg gtaaaacctt cagtggtaag4320
gactggagtc tgacagcccg tgccggcctg cattatgagt tcgatctgac ggacagtgct4380
gacgttcatc tgaaggatgc agcgggagaa catcagatta atggcagaaa agacagtcgt4440
atgctttacg gtgtggggtt aaatgcccgg tttggcgaca atacgcgtct ggggctggaa4500
gttgaacgct ctgcatttgg taaatacaac acagatgatg cgataaacgc taatattcgt4560
tattcattct ga 4572
<210>83
<211>3921
<212>DNA
<213>大肠杆菌
<400>83
atgaataaaa tatacgcatt aaaatattgt cacgcgacag gggggctgat tgctgtatcc60
gaactggcct ccagagttat gaagaaagcc gctcgcggca gccttttagc attatttaat120
ctatcattgt atggtgcttt tttaagcgca tctcaggctg ctcaactaaa tattgataat180
gtatgggcta gagattattt agacctcgca caaaataagg gggtgtttaa agctggtgcg240
accaatgttt caattcaact caagaatggc cagacgttta attttccaaa tgttccaatt300
cctgatttct cgccggcctc aaataaaggc gctactacat ctataggtgg agcttatagt360
gtcacagcaa cccataacgg aacaactcat catgcaataa gcacccaaaa ctggggacaa420
agctcatata aatatataga ccggatgacg aatggagatt ttgctgtaac acgacttgat480
aagtttgttg ttgaaacaac aggggtaaaa aattcagtag atttttctct caatagtcat540
gatgctcttg aacgttatgg tgtggagatc aatggtgaga aaaaaatcat tggtttcagg600
gttggggctg ggacgactta taccgctcaa aatggtaata catatagtac aggacaggta660
tacaatcctc ttttgttaag cgcttcaatg tttcagttaa actgggataa caaaagacca720
tataataaca cgacaccttt ttataatgaa actaccggtg gagacagtgg ttccggtttc780
tatctgtatg ataacgtaaa aaaagaatgg gttatgcttg gtactttatt tggaatagca840
tccagtggtg cagatgttgg gtctattctg aatcagtatg atgaaaatac agttaatggt900
ttaaaaaaca aatttactca aaaagtccag ttaaacaata atacaatgtc gcttaatagt960
gacagtttta cgttagctgg taataataca gcagtggcaa aaaataataa taactataaa1020
gatctaagtt ttagtggtgg tggaagtatt aatttcgaca atgacgtaaa cattggctct1080
ggtggtctca tttttgatgc agggcatcat tatactgtca ctggtaataa taaaacattc1140
aagggtgccg ggctggatat tggtgacaat actacagtcg actggaatgt gaaaggggtt1200
gtcggtgata acctgcataa aattggtgca ggtacattga atgttaatgt ttctcaaggt1260
aataatctta aaacggggga tggtcttgtc gtattaaata gcgctaatgc atttgataat1320
atttatatgg ccagtggtca tggtgttgta aaaattaatc atagtgcagc gcttaaccag1380
aacaatgact atagaggtat tttctttact gaaaatggtg gtactctgga tttaaatggt1440
tatgaccaga gttttaataa aattgcagcg acagatatag gagcactcat aacaaatagt1500
gcagtgcaga aagcagttct ttctgttaat aatcagtcac actatatgta tcatggttct1560
gtttcaggta atacagagat aaaccaccag tttgataccc aaaaaaataa tagtcgcctg1620
attctggacg gtaatgtcga tattacaaat gacattaaca ttaagaatag ccagctccac1680
catgcaggga catgcctaca tctcatgctg tttttagaga gggtgggggt tacctgcatg1740
ctgccaggag ttatttgtga aaaggattat gtttcaggca tacagcaaca ggaaaactca1800
gccaataaaa ataataatac agattataag accaataatc aggtatcatc atttgagcaa1860
cctgactggg aaaatcgtct gtttaagttt aagacattga atctgataaa ttcagatttt1920
atcgttggcc gtaatgctat tgttgttggt gatatttctg ccaataattc cactctgtct1980
ttaagtggaa aagatacaaa agtacatatt gatatgtatg acggcaaaaa catcacggga2040
gatggcttcg gttttcggca ggatattaaa gatggtgtat ctgtttctcc tgagagcagc2100
agttattttg gaaatgttac gctgaataat cactcattac tggatattgg taataaattt2160
accggtggta tcgaggctta tgacagctcc gtgagtgtga cctcacagaa tgctgttttt2220
gatcgtgttg gcagctttgt caacagcagc ctgaccctcg aaaaaggagc aaaactaacg2280
gctcagggcg gtattttcag caccggggct gtggacgtaa aagaaaatgc ctccctgatc2340
ctgacgggga caccttctgc acagaaacag gagtattact cccctgtgat ttctacaacg2400
gaagggatta acctcggaga taaggccagc ctttctgtta aaaacatggg ctatctgagt2460
tcggatattc atgcaggaac cacggcggca accattaatc tgggagacgg tgatgctgag2520
acggattctc cgttattcag ctccctgata aggggatata acgcggttct gagtggcaac2580
attacgggtg agcagagtac ggtaaatatg aacaatgctc tgtggtactc tgacggaaac2640
tcaacgatcg gaacgctgaa gagtacgggg ggacgagttg aactgggggg cgggaaagac2700
tttgccaccc tgcgggtaaa agagcttaac gcaaataacg ccacattcct gatgcatacc2760
aacaccagtc aggctgacca gctgaatgtc acgaataaac tgttgggcag taataatacc2820
gtcctggtcg actttttaaa caagccagcc agtgaaatga acgtgacgtt aattaccgca2880
ccgaaaggga gtgacgagaa aacgttcact gcaggaacgc agcagattgg tttcagtaat2940
gtcacgccgg taatcagcac agaaaaaacg gatgatgcca caaaatggat gctgacaggg3000
tatcagaccg tctctgatgc cggtgcctcg aaaaccgcaa cggactttat ggcgtcaggt3060
tataaatcct tcctgacaga ggtcaataat ctgaacaagc gtatgggtga cctgcgggat3120
actcaggggg atgccggcgt ctgggcgcgc atcatgaacg gtaccggttc ggcagatggt3180
ggttacagcg ataactacac tcacgttcag attggtgccg acagaaagca tgagctggac3240
ggtgtggatt tgttcacggg tgcattactg acctatacag acagcaatgc aagcagccac3300
gccttcagtg gtaaaaccaa atccgtgggg ggagggttgt acgcttcagc actctttgat3360
tccggggctt attttgacct gattggtaaa tatctccatc acgacaatca gtacacggcg3420
agttttgcgt ctcttggtac aaaagactac agctctcatt cctggtatgc cggtgcagag3480
gtcgggtatc gttaccacct gtcggaagag tcctgggtgg agccacagat ggagctggtt3540
tacggttctg tgtcaggaaa atcttttatc tgggaagacc ggggaatggc cctgagcatg3600
aaagacaagg attataaccc actgattggc cgtaccggtg ttgacgtggg aagaaccttc3660
tccggagacg actggaaaat taccgcgcga gccgggctgg gttaccagtt cgacctgctg3720
gcgaacggag aaacggttct gcgggatgca tccggagaga aacgttttga aggtgaaaag3780
gacagcagaa tgctgatgaa tgtggggatg aatgcggaaa ttaaggataa tatgcgtttt3840
ggcttggagc tggaaaaatc ggcgttcggg aaatataacg tggacaatgc gataaacgct3900
aacttccgtt attctttctg a 3921
<210>84
<211>3207
<212>DNA
<213>大肠杆菌
<400>84
atgaataaaa tatacgcatt aaaatattgt cacgcgacag gggggctgat tgctgtatcc60
gaactggcct ccagagttat gaagaaagcc gctcgcggca gccttttagc attatttaat120
ctatcattgt atggtgcttt tttaagcgca tctcaggctg ggagctccgc aggatccggc180
agcggtttaa aaaacaaatt tactcaaaaa gtccagttaa acaataatac aatgtcgctt240
aatagtgaca gttttacgtt agctggtaat aatacagcag tggcaaaaaa taataataac300
tataaagatc taagttttag tggtggtgga agtattaatt tcgacaatga cgtaaacatt360
ggctctggtg gtctcatttt tgatgcaggg catcattata ctgtcactgg taataataaa420
acattcaagg gtgccgggct ggatattggt gacaatacta cagtcgactg gaatgtgaaa480
ggggttgtcg gtgataacct gcataaaatt ggtgcaggta cattgaatgt taatgtttct540
caaggtaata atcttaaaac gggggatggt cttgtcgtat taaatagcgc taatgcattt600
gataatattt atatggccag tggtcatggt gttgtaaaaa ttaatcatag tgcagcgctt660
aaccagaaca atgactatag aggtattttc tttactgaaa atggtggtac tctggattta720
aatggttatg accagagttt taataaaatt gcagcgacag atataggagc actcataaca780
aatagtgcag tgcagaaagc agttctttct gttaataatc agtcacacta tatgtatcat840
ggttctgttt caggtaatac agagataaac caccagtttg atacccaaaa aaataatagt900
cgcctgattc tggacggtaa tgtcgatatt acaaatgaca ttaacattaa gaatagccag960
ctccaccatg cagggacatg cctacatctc atgctgtttt tagagagggt gggggttacc1020
tgcatgctgc caggagttat ttgtgaaaag gattatgttt caggcataca gcaacaggaa1080
aactcagcca ataaaaataa taatacagat tataagacca ataatcaggt atcatcattt1140
gagcaacctg actgggaaaa tcgtctgttt aagtttaaga cattgaatct gataaattca1200
gattttatcg ttggccgtaa tgctattgtt gttggtgata tttctgccaa taattccact1260
ctgtctttaa gtggaaaaga tacaaaagta catattgata tgtatgacgg caaaaacatc1320
acgggagatg gcttcggttt tcggcaggat attaaagatg gtgtatctgt ttctcctgag1380
agcagcagtt attttggaaa tgttacgctg aataatcact cattactgga tattggtaat1440
aaatttaccg gtggtatcga ggcttatgac agctccgtga gtgtgacctc acagaatgct1500
gtttttgatc gtgttggcag ctttgtcaac agcagcctga ccctcgaaaa aggagcaaaa1560
ctaacggctc agggcggtat tttcagcacc ggggctgtgg acgtaaaaga aaatgcctcc1620
ctgatcctga cggggacacc ttctgcacag aaacaggagt attactcccc tgtgatttct1680
acaacggaag ggattaacct cggagataag gccagccttt ctgttaaaaa catgggctat1740
ctgagttcgg atattcatgc aggaaccacg gcggcaacca ttaatctggg agacggtgat1800
gctgagacgg attctccgtt attcagctcc ctgataaggg gatataacgc ggttctgagt1860
ggcaacatta cgggtgagca gagtacggta aatatgaaca atgctctgtg gtactctgac1920
ggaaactcaa cgatcggaac gctgaagagt acggggggac gagttgaact ggggggcggg1980
aaagactttg ccaccctgcg ggtaaaagag cttaacgcaa ataacgccac attcctgatg2040
cataccaaca ccagtcaggc tgaccagctg aatgtcacga ataaactgtt gggcagtaat2100
aataccgtcc tggtcgactt tttaaacaag ccagccagtg aaatgaacgt gacgttaatt2160
accgcaccga aagggagtga cgagaaaacg ttcactgcag gaacgcagca gattggtttc2220
agtaatgtca cgccggtaat cagcacagaa aaaacggatg atgccacaaa atggatgctg2280
acagggtatc agaccgtctc tgatgccggt gcctcgaaaa ccgcaacgga ctttatggcg2340
tcaggttata aatccttcct gacagaggtc aataatctga acaagcgtat gggtgacctg2400
cgggatactc agggggatgc cggcgtctgg gcgcgcatca tgaacggtac cggttcggca2460
gatggtggtt acagcgataa ctacactcac gttcagattg gtgccgacag aaagcatgag2520
ctggacggtg tggatttgtt cacgggtgca ttactgacct atacagacag caatgcaagc2580
agccacgcct tcagtggtaa aaccaaatcc gtggggggag ggttgtacgc ttcagcactc2640
tttgattccg gggcttattt tgacctgatt ggtaaatatc tccatcacga caatcagtac2700
acggcgagtt ttgcgtctct tggtacaaaa gactacagct ctcattcctg gtatgccggt2760
gcagaggtcg ggtatcgtta ccacctgtcg gaagagtcct gggtggagcc acagatggag2820
ctggtttacg gttctgtgtc aggaaaatct tttatctggg aagaccgggg aatggccctg2880
agcatgaaag acaaggatta taacccactg attggccgta ccggtgttga cgtgggaaga2940
accttctccg gagacgactg gaaaattacc gcgcgagccg ggctgggtta ccagttcgac3000
ctgctggcga acggagaaac ggttctgcgg gatgcatccg gagagaaacg ttttgaaggt3060
gaaaaggaca gcagaatgct gatgaatgtg gggatgaatg cggaaattaa ggataatatg3120
cgttttggct tggagctgga aaaatcggcg ttcgggaaat ataacgtgga caatgcgata3180
aacgctaact tccgttattc tttctga 3207
<210>85
<211>3489
<212>DNA
<213>大肠杆菌/结核分枝杆菌
<400>85
atgaataaaa tatacgcatt aaaatattgt cacgcgacag gggggctgat tgctgtatcc60
gaactggcct ccagagttat gaagaaagcc gctcgcggca gccttttagc attatttaat120
ctatcattgt atggtgcttt tttaagcgca tctcaggctg ggagctccat gaccgaacag180
cagtggaact tcgcgggtat cgaagcggcg gcgagcgcga tccagggtaa cgtgaccagc240
atccacagcc tgctggatga aggtaaacag agcctgacca aactggcggc ggcctggggt300
ggtagcggta gcgaagcgta tcagggtgtg cagcagaaat gggatgcgac cgcgaccgaa360
ctgaacaacg cgctgcagaa cctggcgcgt accatcagcg aagcgggtca ggcgatggcg420
agcaccgaag gtaacgtgac cggtatgttc gcgggatccg gcagcggttt aaaaaacaaa480
tttactcaaa aagtccagtt aaacaataat acaatgtcgc ttaatagtga cagttttacg540
ttagctggta ataatacagc agtggcaaaa aataataata actataaaga tctaagtttt600
agtggtggtg gaagtattaa tttcgacaat gacgtaaaca ttggctctgg tggtctcatt660
tttgatgcag ggcatcatta tactgtcact ggtaataata aaacattcaa gggtgccggg720
ctggatattg gtgacaatac tacagtcgac tggaatgtga aaggggttgt cggtgataac780
ctgcataaaa ttggtgcagg tacattgaat gttaatgttt ctcaaggtaa taatcttaaa840
acgggggatg gtcttgtcgt attaaatagc gctaatgcat ttgataatat ttatatggcc900
agtggtcatg gtgttgtaaa aattaatcat agtgcagcgc ttaaccagaa caatgactat960
agaggtattt tctttactga aaatggtggt actctggatt taaatggtta tgaccagagt1020
tttaataaaa ttgcagcgac agatatagga gcactcataa caaatagtgc agtgcagaaa1080
gcagttcttt ctgttaataa tcagtcacac tatatgtatc atggttctgt ttcaggtaat1140
acagagataa accaccagtt tgatacccaa aaaaataata gtcgcctgat tctggacggt1200
aatgtcgata ttacaaatga cattaacatt aagaatagcc agctccacca tgcagggaca1260
tgcctacatc tcatgctgtt tttagagagg gtgggggtta cctgcatgct gccaggagtt1320
atttgtgaaa aggattatgt ttcaggcata cagcaacagg aaaactcagc caataaaaat1380
aataatacag attataagac caataatcag gtatcatcat ttgagcaacc tgactgggaa1440
aatcgtctgt ttaagtttaa gacattgaat ctgataaatt cagattttat cgttggccgt1500
aatgctattg ttgttggtga tatttctgcc aataattcca ctctgtcttt aagtggaaaa1560
gatacaaaag tacatattga tatgtatgac ggcaaaaaca tcacgggaga tggcttcggt1620
tttcggcagg atattaaaga tggtgtatct gtttctcctg agagcagcag ttattttgga1680
aatgttacgc tgaataatca ctcattactg gatattggta ataaatttac cggtggtatc1740
gaggcttatg acagctccgt gagtgtgacc tcacagaatg ctgtttttga tcgtgttggc1800
agctttgtca acagcagcct gaccctcgaa aaaggagcaa aactaacggc tcagggcggt1860
attttcagca ccggggctgt ggacgtaaaa gaaaatgcct ccctgatcct gacggggaca1920
ccttctgcac agaaacagga gtattactcc cctgtgattt ctacaacgga agggattaac1980
ctcggagata aggccagcct ttctgttaaa aacatgggct atctgagttc ggatattcat2040
gcaggaacca cggcggcaac cattaatctg ggagacggtg atgctgagac ggattctccg2100
ttattcagct ccctgataag gggatataac gcggttctga gtggcaacat tacgggtgag2160
cagagtacgg taaatatgaa caatgctctg tggtactctg acggaaactc aacgatcgga2220
acgctgaaga gtacgggggg acgagttgaa ctggggggcg ggaaagactt tgccaccctg2280
cgggtaaaag agcttaacgc aaataacgcc acattcctga tgcataccaa caccagtcag2340
gctgaccagc tgaatgtcac gaataaactg ttgggcagta ataataccgt cctggtcgac2400
tttttaaaca agccagccag tgaaatgaac gtgacgttaa ttaccgcacc gaaagggagt2460
gacgagaaaa cgttcactgc aggaacgcag cagattggtt tcagtaatgt cacgccggta2520
atcagcacag aaaaaacgga tgatgccaca aaatggatgc tgacagggta tcagaccgtc2580
tctgatgccg gtgcctcgaa aaccgcaacg gactttatgg cgtcaggtta taaatccttc2640
ctgacagagg tcaataatct gaacaagcgt atgggtgacc tgcgggatac tcagggggat2700
gccggcgtct gggcgcgcat catgaacggt accggttcgg cagatggtgg ttacagcgat2760
aactacactc acgttcagat tggtgccgac agaaagcatg agctggacgg tgtggatttg2820
ttcacgggtg cattactgac ctatacagac agcaatgcaa gcagccacgc cttcagtggt2880
aaaaccaaat ccgtgggggg agggttgtac gcttcagcac tctttgattc cggggcttat2940
tttgacctga ttggtaaata tctccatcac gacaatcagt acacggcgag ttttgcgtct3000
cttggtacaa aagactacag ctctcattcc tggtatgccg gtgcagaggt cgggtatcgt3060
taccacctgt cggaagagtc ctgggtggag ccacagatgg agctggttta cggttctgtg3120
tcaggaaaat cttttatctg ggaagaccgg ggaatggccc tgagcatgaa agacaaggat3180
tataacccac tgattggccg taccggtgtt gacgtgggaa gaaccttctc cggagacgac3240
tggaaaatta ccgcgcgagc cgggctgggt taccagttcg acctgctggc gaacggagaa3300
acggttctgc gggatgcatc cggagagaaa cgttttgaag gtgaaaagga cagcagaatg3360
ctgatgaatg tggggatgaa tgcggaaatt aaggataata tgcgttttgg cttggagctg3420
gaaaaatcgg cgttcgggaa atataacgtg gacaatgcga taaacgctaa cttccgttat3480
tctttctga 3489
<210>86
<211>19
<212>DNA
<213>人工序列
<220>
<223>引物
<400>86
gaacatcgga aggtggtgc 19
<210>87
<211>20
<212>DNA
<213>人工序列
<220>
<223>引物
<400>87
gagaaaccga atccttaagg 20
<210>88
<211>20
<212>DNA
<213>人工序列
<220>
<223>引物
<400>88
ggatggttgt gttcagtgtg 20
<210>89
<211>61
<212>DNA
<213>人工序列
<220>
<223>引物
<400>89
taactttcta gattacaaaa cttaggaggg tttttaccat gaacagaatt tattctcttc60
g61
<210>90
<211>33
<212>DNA
<213>人工序列
<220>
<223>引物
<400>90
cagtgaattc tcagaatgaa taacgaatat tag 33
<210>91
<211>48
<212>DNA
<213>人工序列
<220>
<223>引物
<400>91
gggagctcct gcggatccgg cagcggtaat gatgccccgg tcacgttc 48
<210>92
<211>38
<212>DNA
<213>人工序列
<220>
<223>引物
<400>92
cggatccgca ggagctcccc gcaagacttc ctgcagag 38
<210>93
<211>51
<212>DNA
<213>人工序列
<220>
<223>引物
<400>93
ctgggagctc cgcaggatcc ggcagcggta atactgcagg gtatctgttt c 51
<210>94
<211>49
<212>DNA
<213>人工序列
<220>
<223>引物
<400>94
ctgccggatc ctgcggagct cccagaaccg gcatagtcca gcgtgatag 49
<210>95
<211>48
<212>DNA
<213>人工序列
<220>
<223>引物
<400>95
gggagctcct gcggatccgg cagcggtgca gacaaactgg tgataaac 48
<210>96
<211>19
<212>DNA
<213>人工序列
<220>
<223>引物
<400>96
gttcatcgac cactgggtg 19
<210>97
<211>54
<212>DNA
<213>人工序列
<220>
<223>引物
<400>97
gggagcggga gctccgcagg atccggcagc ggtaaccgca gttttacctt tgac 54
<210>98
<211>48
<212>DNA
<213>人工序列
<220>
<223>引物
<400>98
accgctgccg gatcctgcgg agctcccgct cccctgcagc gtcagacg 48
<210>99
<211>21
<212>DNA
<213>人工序列
<220>
<223>引物
<400>99
gcaatctgaa tgtggacaat c 21
<210>100
<211>52
<212>DNA
<213>人工序列
<220>
<223>引物
<400>100
gggagcggga gctccgcagg atccggcagc ggtagtgtct tcaacggcac cg 52
<210>101
<211>52
<212>DNA
<213>人工序列
<220>
<223>引物
<400>101
accgctgccg gatcctgcgg agctcccgct cccgtcgccc agcgtgacgc tg 52
<210>102
<211>51
<212>DNA
<213>人工序列
<220>
<223>引物
<400>102
gggagcggga gctccgcagg atccggcagc gggtaccgca atatctggag c 51
<210>103
<211>46
<212>DNA
<213>人工序列
<220>
<223>引物
<400>103
gctgccggat cctgcggagc tcccgctccc tccgagggtg acagtc 46
<210>104
<211>19
<212>DNA
<213>人工序列
<220>
<223>引物
<400>104
gtcatgacct gttgccgac 19
<210>105
<211>50
<212>DNA
<213>人工序列
<220>
<223>引物
<400>105
ctgggagctc cgcaggatcc ggcagcggta aaagtgtctt caacggcacc 50
<210>106
<211>50
<212>DNA
<213>人工序列
<220>
<223>引物
<400>106
ctgccggatc ctgcggagct cccagaacct gcaacagatg tgccttcttc 50
<210>107
<211>55
<212>DNA
<213>人工序列
<220>
<223>引物
<400>107
gggagcggga gctccgcagg atccggcagc ggtaccgtca acctggataa tcagt 55
<210>108
<211>56
<212>DNA
<213>人工序列
<220>
<223>引物
<400>108
accgctgccg gatcctgcgg agctcccgct cccgccgttg aagacacttt tatctg 56
<210>109
<211>30
<212>DNA
<213>人工序列
<220>
<223>引物
<400>109
cggggagctc cgtgatagcg ggcgtcgacc 30
<210>110
<211>31
<212>DNA
<213>人工序列
<220>
<223>引物
<400>110
tgccggatcc gtgaaactgg ttcaatccca g 31
<210>111
<211>32
<212>DNA
<213>人工序列
<220>
<223>引物
<400>111
cggggagctc catgtcgcaa atcatgtaca ac 32
<210>112
<211>27
<212>DNA
<213>人工序列
<220>
<223>引物
<400>112
tgccggatcc gccgccccat ttggcgg 27
<210>113
<211>27
<212>DNA
<213>人工序列
<220>
<223>引物
<400>113
cggggagctc cttctcccgg ccggggc 27
<210>114
<211>27
<212>DNA
<213>人工序列
<220>
<223>引物
<400>114
tgccggatcc gccggcgcct aacgaac 27
<210>115
<211>30
<212>DNA
<213>人工序列
<220>
<223>引物
<400>115
cggggagctc caccggcagc gctgcaatcg 30
<210>116
<211>29
<212>DNA
<213>人工序列
<220>
<223>引物
<400>116
tgccggatcc cgacaagccg attgcagcg 29
<210>117
<211>64
<212>DNA
<213>人工序列
<220>
<223>引物
<400>117
taactttcta gattacaaaa cttaggaggg tttttaccat gaataaaata tacgcattaa60
aata64
<210>118
<211>33
<212>DNA
<213>人工序列
<220>
<223>引物
<400>118
gtcagaattc tcagaaagaa taacggaagt tag 33
<210>119
<211>54
<212>DNA
<213>人工序列
<220>
<223>引物
<400>119
gggagctccg caggatccgg cagcggttta aaaaacaaat ttactcaaaa agtc 54
<210>120
<211>42
<212>DNA
<213>人工序列
<220>
<223>引物
<400>120
cggatcctgc ggagctccca gcctgagatg cgcttaaaaa ag 42
<210>121
<211>20
<212>DNA
<213>人工序列
<220>
<223>引物
<400>121
ccagagccaa tgtttacgtc 20
<210>122
<211>30
<212>DNA
<213>人工序列
<220>
<223>引物
<400>122
gtacgaattc gtgcgtaacg gcaaaagcac 30
<210>123
<211>30
<212>DNA
<213>人工序列
<220>
<223>引物
<400>123
gtacgtcgac acatgagcag atcctctacg 30
<210>124
<211>25
<212>DNA
<213>人工序列
<220>
<223>引物
<400>124
agaaaaacuc atcgagcatc aaatg 25
<210>125
<211>23
<212>DNA
<213>人工序列
<220>
<223>引物
<400>125
atgagccaua ttcaacggga aac 23
<210>126
<211>31
<212>DNA
<213>人工序列
<220>
<223>引物
<400>126
agtttttcua actgtcagac caagtttact c 31
<210>127
<211>37
<212>DNA
<213>人工序列
<220>
<223>引物
<400>127
atggctcaua ctcttccttt ttcaatatta ttgaagc 37
<210>128
<211>23
<212>DNA
<213>人工序列
<220>
<223>引物
<400>128
atctttcugc gaattgagat gac 23
<210>129
<211>20
<212>DNA
<213>人工序列
<220>
<223>引物
<400>129
aagcctaguc tcatgagcgg 20
<210>130
<211>32
<212>DNA
<213>人工序列
<220>
<223>引物
<400>130
actaggctug taatcatggt catagctgtt tc 32
<210>131
<211>30
<212>DNA
<213>人工序列
<220>
<223>引物
<400>131
agaaagauag acgaaagggc ctcgtgatac 30
<210>132
<211>91
<212>PRT
<213>噬菌体phiX174
<400>132
Met Val Arg Trp Thr Leu Trp Asp Thr Leu Ala Phe Leu Leu Leu Leu
1 5 10 15
Ser Leu Leu Leu Pro Ser Leu Leu Ile Met Phe Ile Pro Ser Thr Phe
20 25 30
Lys Arg Pro Val Ser Ser Trp Lys Ala Leu Asn Leu Arg Lys Thr Leu
35 40 45
Leu Met Ala Ser Ser Val Arg Leu Lys Pro Leu Asn Cys Ser Arg Leu
50 55 60
Pro Cys Val Tyr Ala Gln Glu Thr Leu Thr Phe Leu Leu Thr Gln Lys
65 70 75 80
Lys Thr Cys Val Lys Asn Tyr Val Arg Lys Glu
85 90
<210>133
<211>295
<212>DNA
<213>人工序列
<220>
<223>编码裂解蛋白E的合成性DNA片段
<400>133
gaattcacca tggtacgctg gactttgtgg gataccctcg ctttcctgct cctgttgagt60
ttattgctgc cgtcattgct tattatgttc atcccgtcaa cattcaaacg gcctgtctca120
tcatggaagg cgctgaattt acggaaaaca ttattaatgg cgtcgagcgt ccggttaaag180
ccgctgaatt gttcgcgttt accttgcgtg tacgcgcagg aaacactgac gttcttactg240
acgcagaaga aaacgtgcgt caaaaattac gtgcggaagg agtgataagg gatcc 295
<210>134
<211>30
<212>DNA
<213>人工序列
<220>
<223>引物
<400>134
gcgcagtact ttgcgccatt ctatggtgtc 30
<210>135
<211>32
<212>DNA
<213>人工序列
<220>
<223>引物
<400>135
gcgcagtact cacagcatca gaatgaataa cg 32
<210>136
<211>20
<212>DNA
<213>人工序列
<220>
<223>引物
<400>136
tataaccctt gtcgccgttg 20
<210>137
<211>20
<212>DNA
<213>人工序列
<220>
<223>引物
<400>137
acgcagcaag gtcgatttac 20

Claims (44)

1.一种能够表达多于一种感兴趣多肽POI的宿主细胞，该宿主细胞是革兰氏阴性细菌且包含融合蛋白或编码融合蛋白的核酸，所述融合蛋白包含所述POI以及
i.一种包含来自属于pfam自转运蛋白家族PF03797的自转运蛋白的β茎部结构域的载客结构域，其中该载客结构域的β茎部形成序列是基本上完整的，所述β茎部形成序列是基本上完整的是指与破坏或完全除去β茎部时相比，蛋白质的分泌功能的效率维持在最佳水平并且也指在分泌后，载客结构域的稳定性得到维持；
ii.一种来自属于pfam自转运蛋白家族PF03797的自转运蛋白的易位蛋白结构域；和
iii.一种能够将该融合蛋白靶向革兰氏阴性细菌内膜的信号肽；
其中处于其天然形式的自转运蛋白的载客结构域包含至少两个侧面结构域，并且其中至少两种POI插入、替代或部分替代不同的侧面结构域。
2.根据权利要求1所述的宿主细胞，其中在表达时，该融合蛋白在该细胞表面展示。
3.根据权利要求1-2中任一项所述的宿主细胞，其中在表达时，该融合蛋白从该细胞表面分泌并且释放。
4.根据权利要求1-2中任一项所述的宿主细胞，其中i)中的载客结构域和ii)中的易位蛋白结构域源自一种肠杆菌科的丝氨酸蛋白酶自转运蛋白SPATE。
5.根据权利要求所述4的宿主细胞，其中该SPATE蛋白是来自大肠杆菌的血红蛋白结合蛋白酶Hbp、胞外丝氨酸蛋白酶EspC或温度敏感性血凝素Tsh。
6.根据权利要求5所述的宿主细胞，其中该SPATE蛋白由序列为SEQ ID NO 1或SEQ IDNO 2的多肽组成。
7.根据权利要求6所述的宿主细胞，其中SEQ ID NO 1或SEQ ID NO 2的氨基酸53-308、533-608、657-697、735-766和898-922对应于多个侧面结构域，并且其中这些POI插入、替代或部分替代至少两个这类侧面结构域。
8.一种融合蛋白，其包含
i.多于一种感兴趣多肽POI；
ii.一种包含来自属于pfam自转运蛋白家族PF03797的自转运蛋白的β茎部结构域的载客结构域，其中该载客结构域的β茎部形成序列是基本上完整的，所述β茎部形成序列是基本上完整的是指与破坏或完全除去β茎部时相比，蛋白质的分泌功能的效率维持在最佳水平并且也指在分泌后，载客结构域的稳定性得到维持；
iii.一种来自属于pfam自转运蛋白家族PF03797的自转运蛋白的易位蛋白结构域；和
iv.可任选地，一种将该融合蛋白靶向革兰氏阴性细菌内膜的信号肽，
其中处于其天然形式的自转运蛋白的载客结构域包含至少两个侧面结构域，并且其中至少两种POI替代或部分替代独立的侧面结构域。
9.根据权利要求8所述的融合蛋白，其中i)中的载客结构域和ii)中的易位蛋白结构域源自一种肠杆菌科的丝氨酸蛋白酶自转运蛋白SPATE。
10.根据权利要求9所述的融合蛋白，其中该SPATE蛋白是来自大肠杆菌的血红蛋白结合蛋白酶Hbp、胞外丝氨酸蛋白酶EspC或温度敏感性血凝素Tsh。
11.根据权利要求10所述的融合蛋白，其中该SPATE蛋白由序列为SEQ ID NO 1或SEQID NO 2的多肽组成。
12.根据权利要求11所述的融合蛋白，其中SEQ ID NO 1或SEQ ID NO 2的氨基酸53-308、533-608、657-697、735-766和898-922对应于多个侧面结构域，并且其中这些POI插入、替代或部分替代至少两个这类侧面结构域。
13.一种经安排用于表达融合蛋白的核酸，所述核酸在框内包含：
i.编码所述融合蛋白的信号肽的序列，该信号肽能够将该融合蛋白靶向革兰氏阴性细菌的内膜；
ii.编码所述融合蛋白的载客结构域的序列，该载客结构域包含来自属于pfam自转运蛋白家族PF03797的自转运蛋白的β茎部结构域；以及
iii.编码所述融合蛋白的易位蛋白结构域的序列，该易位蛋白结构域源自属于pfam自转运蛋白家族PF03797的自转运蛋白，
其中该编码处于其天然形式的自转运蛋白的载客结构域的序列包含至少两段序列，这些序列编码从该β茎部结构域突出的侧面结构域，并且其中该编码载客结构域的序列包含允许框内地克隆至少两个编码感兴趣多肽POI的DNA序列的至少两段克隆位点序列，所述至少两段克隆位点序列插入、替代或部分地替代所述编码侧面结构域的序列段的独立段，并且所述克隆位点序列段如此安排，从而该载客结构域的所编码的β茎部形成蛋白序列是基本上完整的，所述β茎部形成序列是基本上完整的是指与破坏或完全除去β茎部时相比，蛋白质的分泌功能的效率维持在最佳水平并且也指在分泌后，载客结构域的稳定性得到维持。
14.一种经安排用于表达融合蛋白的核酸，所述核酸在框内包含：
i.编码所述融合蛋白的信号肽的序列，该信号肽能够将该融合蛋白靶向革兰氏阴性细菌的内膜；
ii.编码所述融合蛋白的载客结构域的序列，该载客结构域包含来自属于pfam自转运蛋白家族PF03797的自转运蛋白的β茎部结构域；
iii.编码所述融合蛋白的易位蛋白结构域的序列，该易位蛋白结构域源自属于pfam自转运蛋白家族PF03797的自转运蛋白；和
iv.编码所述融合蛋白的多于一种感兴趣多肽POI的序列，
其中这些编码POI的序列与该编码载客结构域的序列融合并且如此安排，从而该载客结构域的所编码的β茎部形成蛋白序列是基本上完整的，所述β茎部形成序列是基本上完整的是指与破坏或完全除去β茎部时相比，蛋白质的分泌功能的效率维持在最佳水平并且也指在分泌后，载客结构域的稳定性得到维持，并且其中该编码处于其天然形式的自转运蛋白的载客结构域的序列包含至少两段序列，这些序列编码从该β茎部结构域突出的侧面结构域，并且这些编码POI的序列的每一个插入、替代或部分地替代所述编码侧面结构域的序列段的独立段。
15.根据权利要求13-14中任一项所述的核酸，进一步包含编码一种切割位点的序列，该切割位点允许该编码的融合蛋白从携带所述核酸的革兰氏阴性细菌中分泌。
16.根据权利要求13-14中任一项所述的核酸，不包含编码一种允许分泌的切割位点的序列，或包含一种破坏的切割位点，从而该编码的融合蛋白经安排以展示于携带所述核酸的革兰氏阴性细菌的细胞表面上。
17.根据权利要求13-14中任一项所述的核酸，其中编码ii)中载客结构域和iii)中易位蛋白结构域的序列源自编码一种肠杆菌科的丝氨酸蛋白酶自转运蛋白SPATE的基因。
18.根据权利要求17所述的核酸，其中该SPATE蛋白是来自大肠杆菌的血红蛋白结合蛋白酶Hbp、胞外丝氨酸蛋白酶EspC或温度敏感性血凝素Tsh。
19.根据权利要求18所述的核酸，其中编码该SPATE蛋白的基因对SEQ ID NO 1或SEQID NO 2的蛋白质序列进行编码。
20.根据权利要求19所述的核酸，其中SEQ ID NO 1或SEQ ID NO 2的氨基酸53-308、533-608、657-697、735-766和898-922对应于多个侧面结构域，并且其中安排这些克隆位点或编码POI的序列以替代或部分地替代至少两个这类侧面结构域。
21.一种载体，其包含根据权利要求13-20中任一项所述的核酸。
22.一种革兰氏阴性细菌，其包含根据权利要求13-20中任一项所述的核酸或根据权利要求21所述的载体。
23.根据权利要求22所述的革兰氏阴性细菌，其选自肠杆菌科(Enterobacteriaceae)。
24.根据权利要求23所述的革兰氏阴性细菌，其选自大肠杆菌(Escherichia coli)、沙门氏菌属物种(Salmonella spp.)、弧菌属物种(Vibrio spp.)、志贺氏菌属物种(Shigellaspp.)、假单胞菌属物种(Pseudomonads spp.)、伯克霍尔德菌属物种(Burkholderia spp.)或德特氏菌属物种(Bordetella spp.)。
25.一种外膜小泡，在其表面上展示根据权利要求8-12中任一项所述的融合蛋白。
26.一种菌影，在其表面上展示根据权利要求8-12中任一项所述的融合蛋白。
27.一种用于融合蛋白的分泌性蛋白表达的方法，该方法包括步骤
i.提供一种根据权利要求1-7中任一项所述的宿主细胞或根据权利要求22-24中任一项所述的革兰氏阴性细菌；
ii.诱导该融合蛋白的表达。
28.根据权利要求27所述的方法，该方法包括另外的步骤：抑制该革兰氏阴性细菌中具有蛋白酶活性的周质酶。
29.根据权利要求28所述的方法，其中该酶是DegP。
30.根据权利要求29所述的方法，其中通过DegP催化位点中的突变抑制DegP。
31.根据权利要求27-30中任一项所述的方法，该方法包括另外的步骤：下调该革兰氏阴性细菌的周质间隙内催化蛋白质中二硫键形成的至少一种酶。
32.根据权利要求31所述的方法，其中该酶是DsbA或DsbB。
33.根据权利要求27-30中任一项所述的方法，其中该融合蛋白以可溶性方式分泌。
34.根据权利要求27-30中任一项所述的方法，其中该融合蛋白展示在该革兰氏阴性细菌的表面上。
35.根据权利要求27-30中任一项所述的方法，该方法包括另外的步骤：诱导小泡从该革兰氏阴性细菌的外膜排出，因此形成在它们的表面上展示该融合蛋白的外膜小泡。
36.根据权利要求27-30中任一项所述的方法，该方法包括另外的步骤：裂解该革兰氏阴性细菌以形成在它们的表面上展示该融合蛋白的菌影。
37.根据权利要求36所述的方法，其中通过使用来自噬菌体PhiX174的致死性裂解基因E进行该裂解。
38.根据权利要求1-2中任一项所述的宿主细胞、根据权利要求22-24中任一项所述的革兰氏阴性细菌、根据权利要求8-12中任一项所述的融合蛋白、根据权利要求13或14所述的核酸、根据权利要求21所述的载体、根据权利要求25所述的外膜小泡、根据权利要求26所述的菌影或根据权利要求27-30中任一项所述的方法，其中这些POI的至少一种包含抗原。
39.根据权利要求38所述的宿主细胞、革兰氏阴性细菌、融合蛋白、核酸、载体、外膜小泡、菌影或方法，其中该抗原是来自感染性生物的抗原。
40.根据权利要求39所述的宿主细胞、革兰氏阴性细菌、核酸、载体、融合蛋白或方法，其中该抗原是来自结核分枝杆菌(Mycobacterium tuberculosis)的抗原。
41.根据权利要求40所述的宿主细胞、革兰氏阴性细菌、核酸、载体、融合蛋白或方法，其中该来自结核分枝杆菌的抗原选自下组，该组由以下各项组成：ESAT-6、Ag85B、Rv2660c、TB10.4和TB10.3。
42.根据权利要求41所述的宿主细胞、革兰氏阴性细菌、核酸、载体、融合蛋白或方法，其中该抗原是Ag85B，所述Ag85B已经分裂成N’部分Ag85B-N’和C’部分Ag85B-C’，并且其中每个部分与来自自转运蛋白的载客结构域的独立侧面结构域融合。
43.根据权利要求41所述的宿主细胞、革兰氏阴性细菌、核酸、载体、融合蛋白或方法，其中抗原ESAT-6、Ag85B、Rv2660c、TB10.4和TB10.3的至少2种各自以分裂或全序列形式融合至、插入、替代或部分替代来自自转运蛋白的载客结构域的独立侧面结构域。
44.一种疫苗，包含根据权利要求38-43中任一项所述的宿主细胞、革兰氏阴性细菌、融合蛋白、外膜小泡或菌影。
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