CN103228781B - 软骨素的生物技术制备 - Google Patents

软骨素的生物技术制备 Download PDF

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Publication number
CN103228781B
CN103228781B CN201180033969.3A CN201180033969A CN103228781B CN 103228781 B CN103228781 B CN 103228781B CN 201180033969 A CN201180033969 A CN 201180033969A CN 103228781 B CN103228781 B CN 103228781B
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China
Prior art keywords
intestinal bacteria
recombinant microorganism
chrondroitin
kfoe
gene
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CN201180033969.3A
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English (en)
Chinese (zh)
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CN103228781A (zh
Inventor
A·特里利
I·布泻洛
S·戴利
F·巴加廷
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lingzhi
Gnosis SpA
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Lingzhi
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Priority claimed from IT001264A external-priority patent/ITMI20101264A1/it
Priority claimed from IT001300A external-priority patent/ITMI20101300A1/it
Application filed by Lingzhi filed Critical Lingzhi
Priority to CN201510177998.7A priority Critical patent/CN104974972B/zh
Publication of CN103228781A publication Critical patent/CN103228781A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0069—Chondroitin-4-sulfate, i.e. chondroitin sulfate A; Dermatan sulfate, i.e. chondroitin sulfate B or beta-heparin; Chondroitin-6-sulfate, i.e. chondroitin sulfate C; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01099—Sucrose:sucrose fructosyltransferase (2.4.1.99)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Materials Engineering (AREA)
  • Dermatology (AREA)
  • Polymers & Plastics (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Materials For Medical Uses (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201180033969.3A 2010-07-09 2011-06-01 软骨素的生物技术制备 Active CN103228781B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201510177998.7A CN104974972B (zh) 2010-07-09 2011-06-01 软骨素的生物技术制备

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
IT001264A ITMI20101264A1 (it) 2010-07-09 2010-07-09 Produzione biotecnologica di condroitina
ITMI2010A001264 2010-07-09
ITMI2010A001300 2010-07-15
IT001300A ITMI20101300A1 (it) 2010-07-15 2010-07-15 Produzione biotecnologica di condroitina
PCT/EP2011/059069 WO2012004063A1 (en) 2010-07-09 2011-06-01 Biotechnological production of chondroitin

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN201510177998.7A Division CN104974972B (zh) 2010-07-09 2011-06-01 软骨素的生物技术制备

Publications (2)

Publication Number Publication Date
CN103228781A CN103228781A (zh) 2013-07-31
CN103228781B true CN103228781B (zh) 2015-04-29

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
CN201180033969.3A Active CN103228781B (zh) 2010-07-09 2011-06-01 软骨素的生物技术制备
CN201510177998.7A Active CN104974972B (zh) 2010-07-09 2011-06-01 软骨素的生物技术制备

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Country Status (25)

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US (2) US8609394B2 (es)
EP (1) EP2591127B1 (es)
JP (1) JP6030056B2 (es)
KR (1) KR101855062B1 (es)
CN (2) CN103228781B (es)
AU (1) AU2011275996B2 (es)
BR (1) BR112013000648A2 (es)
CA (1) CA2801843C (es)
CY (1) CY1119469T1 (es)
DK (1) DK2591127T3 (es)
EA (1) EA025126B1 (es)
ES (1) ES2644443T3 (es)
GE (1) GEP201706720B (es)
HR (1) HRP20171624T1 (es)
IL (1) IL223830B (es)
LT (1) LT2591127T (es)
MX (1) MX2013000100A (es)
NO (1) NO2591127T3 (es)
NZ (1) NZ603990A (es)
PL (1) PL2591127T3 (es)
PT (1) PT2591127T (es)
SG (1) SG186212A1 (es)
SI (1) SI2591127T1 (es)
WO (1) WO2012004063A1 (es)
ZA (1) ZA201209079B (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8697398B2 (en) 2010-03-01 2014-04-15 Dsm Ip Assets B.V. Compositions and methods for bacterial production of chondroitin
US8664196B2 (en) 2011-05-20 2014-03-04 Gnosis S.P.A. Shark-like chondroitin sulphate and process for the preparation thereof
ITMI20120880A1 (it) 2012-05-22 2013-11-23 Gnosis Spa Condroitin 6-solfato biotecnologico a basso peso molecolare dotato di attivita' antiinfiammatoria e antiartritica e suo uso nel trattamento e nella prevenzione dell'osteoartrite
US10196625B2 (en) 2013-09-30 2019-02-05 Seikagaku Corporation Method for improving blood persistence of protein
CN106497845B (zh) * 2016-12-14 2019-08-06 江南大学 一种高产软骨素的重组枯草芽孢杆菌及其应用
US20220333144A1 (en) * 2019-09-10 2022-10-20 Rensselaer Polytechnic Institute Recombinant microorganisms for in vivo production of sulfated glycosaminoglycans
IT202000004564A1 (it) 2020-03-04 2021-09-04 Lesaffre & Cie Processo per la solfatazione diretta di polisaccaridi in solvente ecologicamente accettabile
CN112625147A (zh) * 2020-12-22 2021-04-09 荣成万盈水产科技有限公司 一种利用鱿鱼软骨制备硫酸软骨素的方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008133350A1 (en) * 2007-04-24 2008-11-06 Seikagaku Corporation Chondroitin-producing bacterium and method of producing chondroitin

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7642071B2 (en) 1999-04-01 2010-01-05 The Board Of Regents Of The University Of Oklahoma Methods of expressing gram-negative glycosaminoglycan synthase genes in gram-positive hosts
AU2003100886B4 (en) 2003-05-05 2004-07-29 Agnes Chan A multipurpose garment
JP5081629B2 (ja) 2005-12-15 2012-11-28 生化学工業株式会社 長鎖コンドロイチン糖鎖及びその製造方法並びにコンドロイチン合成の促進方法
US8697398B2 (en) * 2010-03-01 2014-04-15 Dsm Ip Assets B.V. Compositions and methods for bacterial production of chondroitin

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008133350A1 (en) * 2007-04-24 2008-11-06 Seikagaku Corporation Chondroitin-producing bacterium and method of producing chondroitin

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CHIONDROITIN C LYASE IS UNABLE TO CLEAVE FRUCTOSYLATED SEQUENCES INSIDE THE PARTIALLY FRUCTOSYLATED ESCHERICHIA COLI K4 POLYMER;NICOLA VOLPI;《GLYCOCONJUGATE JOURNAL》;20070928;第25卷(第5期);451-457 *
MOLECULAR CLONING AND CHARACTERIZATION OF CHONDROITIN POLYMERASE FROM ESCHERICHIA COLI STRAIN K4;NINOMIYA TOSHIO;《THE JOURNAL OF BIOLOGICAL CHEMISTRY》;20020614;第277卷(第24期);21567-21575 *

Also Published As

Publication number Publication date
DK2591127T3 (da) 2017-11-13
EP2591127A1 (en) 2013-05-15
CA2801843A1 (en) 2012-01-12
EA025126B1 (ru) 2016-11-30
IL223830B (en) 2018-02-28
CA2801843C (en) 2018-10-16
ES2644443T3 (es) 2017-11-29
LT2591127T (lt) 2017-12-11
AU2011275996B2 (en) 2015-03-26
NO2591127T3 (es) 2017-12-30
JP2013531995A (ja) 2013-08-15
HRP20171624T1 (hr) 2017-12-01
GEP201706720B (en) 2017-08-25
ZA201209079B (en) 2014-04-26
KR101855062B1 (ko) 2018-05-09
SG186212A1 (en) 2013-01-30
KR20130091324A (ko) 2013-08-16
US20140073772A1 (en) 2014-03-13
US20120010399A1 (en) 2012-01-12
CN104974972B (zh) 2018-11-02
CY1119469T1 (el) 2018-03-07
WO2012004063A1 (en) 2012-01-12
SI2591127T1 (sl) 2017-12-29
JP6030056B2 (ja) 2016-11-24
CN103228781A (zh) 2013-07-31
PL2591127T3 (pl) 2018-01-31
EA201291455A1 (ru) 2013-05-30
NZ603990A (en) 2014-09-26
US8771992B2 (en) 2014-07-08
US8609394B2 (en) 2013-12-17
CN104974972A (zh) 2015-10-14
AU2011275996A1 (en) 2013-01-10
AU2011275996A2 (en) 2013-01-24
MX2013000100A (es) 2013-03-20
EP2591127B1 (en) 2017-08-02
PT2591127T (pt) 2017-11-13
BR112013000648A2 (pt) 2016-05-31

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