CN103211836A - Composition for treating osteoporosis - Google Patents
Composition for treating osteoporosis Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for treating osteoporosis. The composition comprises the following ingredients in parts by weight: 9-12 parts of calcium agent and 20-30 parts of trehalose. According to the composition disclosed by the invention, a synergistic effect is generated through appropriate proportioning. The composition has an excellent effect on the improvement of osteoporosis. According to the composition disclosed by the invention, the calcium absorption ratio is obviously increased compared with that of like products, so that the composition is applicable to people of all ages and is particularly applicable to middle-aged and elderly people; and production process conditions are stable, so that the composition is suitable for being produced in a large-scale and industrial manner.
Description
Technical field
The present invention relates to the osteoporotic compositions of a kind of treatment, specifically, relate to a kind of calcium preparation.
Background technology
Along with the aging of society, in western countries, the prevalence of osteoporosis occupies first of metabolic osteopathy.The misery that the osteoporosis disease brings patient and family is self-evident.According to statistics, China has approximately at present and surpasses 100,000,000 patients with osteoporosis, expects the year two thousand fifty will be increased to 200,000,000 1 thousand ten thousand people.Cause the factor of increasing people's calcium deficiency: live and work rhythm is accelerated, operating pressure is big and live irregular, the structure that is not careful in one's diet reasonably combined, calcium was taken in and was less than 600 milligrams of persons and can thinks the calcium Deficiency of Intake every day, easily caused the shortage of calcium in the body; Some middle age work strains lack self health consciousness, think little of outdoor activity, and proper interior synthetic vitamin D is reduced, and influence the absorption and the utilization of calcium; Nutrition is taken in unbalanced or is lacked the necessary nutrient of human body.
Many in the present calcium-supplementing preparation based on tablet, capsule, the dosage form quasi-drugs, it is poor to take mouthfeel; This type of calcium-supplementing preparation is subjected to the restriction of formulation characteristic, preparation process requirement and dose, much having the novel material that promotes calcium absorption, improves the bone density function can not use in product, makes that treatment osteoporosis series products upgrades slowly, effect of supplemented calcium does not significantly improve.
At modern's tense working and rhythm of life, be badly in need of that a class scientific formulation is reasonable, bioavailability is high, both safety, efficient, the treatment medicine for treating osteoporosis of taking convenience again.
Summary of the invention
The object of the present invention is to provide a kind of compositions of prevention of osteoporosis.
For achieving the above object, the present invention adopts the component of following weight ratio:
9～12 parts of calcium preparation, 20～30 parts of trehaloses.
Described calcium preparation can be to be fit to any calcium salt of taking in the prior art; Preferably, described calcium preparation is the calcium salt that animal or plant extracts, and also can be in calcium gluconate, calcium carbonate and the calcium lactate one or more.
Preferred, described calcium preparation is the calcium salt that animal or plant extracts.
Further preferred, described calcium preparation is the enzymolysis Os Bovis seu Bubali powder.
The enzymolysis Os Bovis seu Bubali powder that uses among the present invention be according to application number be 201210382353.3,201210382498.3,201210382497.9,201210382496.4,201210382435.8,201210382433.9,201210382432.4, technology among the 201210382431.X, 201210382355.2 patent application makes, perhaps preparation method comprises the steps: that skeleton is pulverized, sterilization; Get Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and lactobacillus helveticus (L.helviticus), three kinds of lactobacillus weight ratios 3: 1: 2; Aggregate and water w/v 1: 3～5, it is 0.05% that lactobacillus accounts for the aggregate weight ratio, adds 6% sucrose of gross weight, 45～50 ℃, fermentation 16h, 120 ℃ of sterilization 30min; Add hydrochloric acid adjust pH 2～3, add pepsin, accounting for the aggregate weight ratio is 0.09%, 37～40 ℃ of hydrolysis temperatures, stir 30min, add alkali and regulate pH value to 6～7, add papain, accounting for the aggregate weight ratio is 0.09%, 50～60 ℃, stir 30min, the heating enzyme denaturing; 6% the glucose that adds gross weight, add leuconostoc cremoris and citric acid, it is 0.04～0.06% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 15～20, fermentation temperature is 38 ℃, and fermentation time is 12h, adds the alkali neutralization, 120 ℃ of sterilizations are drying to obtain.
Conjugated linoleic acid has positive effect to the health of sclerotin, and it can reduce the synthetic of the interior PGE2 of human body, and intravital type-1 insulin like growth factor is increased, and promotes the formation of ossein.For reducing bone resorption, promote the formation of ossein, strengthen the effect of utilizing of the present composition, in the present composition, can add 6～10 parts of conjugated linoleic acids.
Collagen polypeptide digestibility height, and collagen polypeptide is for the improvement of osteoarthrosis disease symptoms and prevent and treat the aspect good effect is also arranged.For strengthening the ossein structure under the low calcium level, prevent collagen decomposition, suppress osteoclast, improve bone strength, thus prevention of osteoporosis.The collagen polypeptide that also can add weight portion 10～12 in the present invention's prescription.
Preferably, collagen polypeptide molecular weight of the present invention is 3000-5000 dalton.
Lysine can combine with calcium and form soluble complexes, improves absorption and calcium in vivo the accumulation of small intestinal to calcium, promotes bone growth, and bone metabolism is had material impact.Lysine can act on organic matrix and strengthen bone strength, increases the bone amount, and osteoporosis is had the better prevention effect.For further promoting the absorption of calcium, can add 4～8 parts of lysines in the compositions of the present invention.
Hyperhomocysteinemiainjury is an osteoporosis generation important risk.Replenish competent vitamin B12 and folic acid and can reduce blood plasma homocysteine level.For further reducing the risk factor that osteoporosis takes place, can add 0.01～0.04 part of vitamin in the present composition.Preferably, described vitamin is VB12 and/or folic acid.
Copper is mainly by relying fluorine acyloxylation enzymatic to advance the crosslinked of collagen protein and elastin laminin in the connective tissue, be form connective tissue essential, in the formation of skeleton, bone mineralising, play an important role.When copper lacks, can influence the ossein structure, cause skeleton fragility to increase, also can cause the active reduction of osteoblast, calcium content of bone is descended.The supplementation with copper element has prevention and treats osteoporotic effect.
Selenium can improve calcium metabolism, increase body to the deposition of the absorption of calcium and bone calcium, reduce body to the absorption of aluminum, reduce the generation of free radical simultaneously, senile osteoporosis is had the certain protection effect.Scarce selenium can cause bone metabolism diseases such as Kaschin-Beck disease, is to cause osteoporotic latent dangerous factor.
For improving the bone metabolism of sufferers of osteoporosis face or middle-aged and elderly people, can add 0.01～0.04 part of mineral in the present composition.Described mineral is copper and/or selenium element preferably.
The various dosage forms that adjuvant such as starch, dextrin, lactose, microcrystalline Cellulose, hydroxypropyl methylcellulose, Polyethylene Glycol, magnesium stearate, micropowder silica gel, xylitol, lactose, glucose, glycine, mannitol, glycine etc. are mixed and made on compositions of the present invention and any or more than one pharmaceuticss, for example, can be made into tablet, slow releasing tablet, drop pill, granule, capsule, microgranule.Preferred dosage form is tablet or granule.
After can pulverizing, the present composition joins in the milk powder for daily drinking.
The production process of the compositions of above-mentioned prevention of osteoporosis is characterized in that may further comprise the steps:
(1) calcium preparation is crossed the 60-100 mesh sieve, siftage is standby;
(2) add conjugated linoleic acid, mix speed: 30 rev/mins, 30 minutes, cross 40 eye mesh screens, obtain fine powder 2.
(3) gained fine powder 1, fine powder 2 mix with trehalose, collagen polypeptide, lysine, vitamin and mineral more according to the above ratio, cross 40 mesh sieves promptly.
Trehalose (as the secretion of interleukin-6 and tumor necrosis factor-a) and promote the expression of intestinal epithelial cell to osteoprotegerin, shows the effect of good protect against osteoporosis by downward modulation and bone metabolism related cytokine.The enzymolysis Os Bovis seu Bubali powder has very high calcareous content, can satisfy human body to calcareous daily demand; Its protein exceeds milk powder 23%; Contain the synthetic indispensable element of sclerotin such as abundant phosphorus, magnesium, zinc, ferrum simultaneously.
Compositions of the present invention produces cooperative effect by suitable proportioning.Improvement to the osteoporosis symptom has splendid effect.Compositions calcium absorptivity of the present invention obviously improves than like product, is fit to each age level people and takes, and is particularly useful for middle-aged and elderly people and takes, and its production process conditional stability is fit to large-scale industrial production.
The test example
Below by clinical testing data, further verify beneficial effect of the present invention:
One, clinical experiment
1 object of observation: filter out 85 routine person in middle and old age's sufferers of osteoporosis face through the detection of dual intensity X line borne densitometers.The osteoporotic diagnostic criteria of Chinese that diagnostic criteria adopts Chinese Gerontological Society to formulate in 1999, the 1～2SD that promptly reduces bone amount peak value reduces for the bone amount, and reduction 2SD is an osteoporosis.41 examples are organized in treatment, male's 15 examples, women's 26 examples.Age reckling 38 years old, maximum 68 years old, average 57.4 years old, bone amount minimizing person 15 examples wherein, slight osteoporosis person's 26 examples; Matched group 44 examples, minimum 42 years old of age, maximum 65 years old, average 55.8 years old, bone amount minimizing person 17 examples wherein, slight osteoporosis person's 27 examples.More than two groups of personnel all get rid of the various acute and chronic diseases that diabetes, hyperthyroidism, kidney disease etc. influence bone metabolism.
2 gauges and method: the Osteocore dual intensity X line borne densitometers (DEXA) that adopts French Medilink company to produce carries out the bone density measurement of lumbar vertebra (L2, L3, L4) and hip (neck of femur, Ward ' s district, greater trochanter) respectively to two groups of personnel before and after treatment.The compositions of the oral embodiment of the invention 7 is organized in treatment, and every day 2 times, each 10g took 6 months continuously, and polyphagia contains calcium diet, suitably increases quantity of motion and sun exposure; Matched group is not obeyed any calcium preparation, and only polyphagia contains calcium diet, suitably increases quantity of motion and sun exposure.All after half a year, check bone density for two groups.
3 results
3.1 lumbar vertebra (L2, L3, L4) and hip (neck of femur, Ward ' s district, greater trochanter) BMD contrast before and after the treatment of treatment group
Lumbar vertebra and bmd of proximal femur compare (g/cm before and after table 1 patient treatment
2)
| The position | n | Before the treatment | After the treatment |
| L 2 | 41 | 0.672±0.065 | 0.698±0.074 |
| L 3 | 41 | 0.678±0.115 | 0.715±0.102 |
| L 4 | 41 | 0.672±0.065 | 0.701±0.076 |
| Neck of femur | 41 | 0.633±0.067 | 0.667±0.063 |
| Ward ' s district | 41 | 0.437±0.085 | 0.465±0.099 |
| Greater trochanter | 41 | 0.585±0.085 | 0.612±0.083 |
Annotate: with comparison P＜0.05 before the medication
More than each position increased by 3.8%, 5.4%, 4.3%, 5.3%, 6.4%, 4.6% respectively according to its BMD of order such as L2, L3, L4, neck of femur, Ward ' s district, greater trochanters.Lumbar vertebra BMD on average increases by 4.5%, and bmd of proximal femur on average increases by 5.4%.Other positions are relatively preceding with treatment except that greater trochanter, and difference all has significance (P＜0.05).And do not find any untoward reaction.Nearly 80% patient phenomenons such as lumbago and skelalgia, sural spasm in the time in 1～2 week of taking medicine alleviate or disappear.
3.2 matched group lumbar vertebra and bmd of proximal femur are relatively
Table 2 matched group lumbar vertebra and bmd of proximal femur are relatively
| The position | n | Before the treatment | After the treatment |
| L 2 | 44 | 0.681±0.051 | 0.680±0.058 |
| L 3 | 44 | 0.686±0.104 | 0.688±0.114 |
| L 4 | 44 | 0.695±0.089 | 0.675±0.091 |
| Neck of femur | 44 | 0.699±0.045 | 0.702±0.056 |
| Ward ' s district | 44 | 0.486±0.097 | 0.479±0.089 |
| Greater trochanter | 44 | 0.601±0.079 | 0.589±0.091 |
Annotate: with relatively P is all＞0.05 before the medication
Each position BMD is changed to L2 and reduces 0.1%, and L3 increases by 0.29%, and L4 reduces 2.8%, and neck of femur increases by 0.4%, and Ward ' s district reduces 1.4%, and greater trochanter reduces 1.9%.Lumbar vertebra BMD decreased average 0.03%, near end of thighbone BMD decreased average 0.96%.Symptoms such as patient's lumbago and skelalgia, sural spasm do not have obvious improvement.
Two, the present composition and take enzymolysis Os Bovis seu Bubali powder, trehalose contrast experiment separately
30 of wean rat, male and female half and half.Set enzymolysis Os Bovis seu Bubali powder group, trehalose group, of the present invention group totally three experimental grouies, every group of 10 white mouse, male and female half and half.Except that the conventional feed of feeding, the enzymolysis Os Bovis seu Bubali powder group 1g bone meal of feeding every day, the trehalose group 3g trehalose of feeding every day, the compositions (containing 1g bone meal, 3g trehalose) of the 10g embodiment 4 that feeds of the present invention group of every day.After feeding 60 days, detect 3 bone densities of rats with left femur, the result is as follows:
Different material is to the influence of rat bone density (BMD)
According to the testing result analysis, three experimental grouies all have the effect of improving rat bone density, and of the present invention group of rat femur three selected bone density and be significantly higher than enzymolysis Os Bovis seu Bubali powder group and trehalose group (P＜0.05).
The specific embodiment
Embodiment 1: get 9 parts of calcium gluconate by weight, 20 parts of trehaloses, mix homogeneously.Add adjuvant, make capsule.
Embodiment 2: get 12 parts of calcium gluconate by weight, 30 parts of trehaloses, mix homogeneously.Add adjuvant, make capsule.
Embodiment 3: get 10 parts of calcium gluconate by weight, 25 parts of trehaloses, mix homogeneously.Add adjuvant, make capsule.
Embodiment 4: get 10 parts of enzymolysis Os Bovis seu Bubali powder by weight, 30 parts of trehaloses, mix homogeneously.Add adjuvant, make electuary.
The preparation method of enzymolysis Os Bovis seu Bubali powder may further comprise the steps in the present embodiment: skeleton is pulverized, sterilization; Get Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and lactobacillus helveticus (L.helviticus), three kinds of lactobacillus weight ratios 3: 1: 2; It is 0.05% that aggregate and water w/v 1: 3, lactobacillus account for the aggregate weight ratio, adds 6% sucrose of gross weight, 45 ℃, and fermentation 16h, 120 ℃ of sterilization 30min; Add hydrochloric acid adjust pH 2, add pepsin, accounting for the aggregate weight ratio is 0.09%, and 37 ℃ of hydrolysis temperatures stir 30min, adds alkali and regulates pH value to 6, adds papain, and accounting for the aggregate weight ratio is 0.09%, 50 ℃, stirs 30min, the heating enzyme denaturing; Add 6% glucose of gross weight, add leuconostoc cremoris and citric acid, it is 0.04% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 15, fermentation temperature are 38 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of sterilizations are drying to obtain.
Embodiment 5: get 9 parts of calcium carbonate by weight, 20 parts of trehaloses, 6 parts of conjugated linoleic acids, mix homogeneously.Add adjuvant, make capsule.
Embodiment 6: extracting lactic acid calcium is 12 parts by weight, 30 parts of trehaloses, 10 parts of conjugated linoleic acids, mix homogeneously.Add adjuvant, make tablet.
Embodiment 7: get 10 parts of enzymolysis Os Bovis seu Bubali powder by weight, 25 parts of trehaloses, 8 parts of conjugated linoleic acids, mix homogeneously.Add adjuvant, make electuary.
The preparation method of enzymolysis Os Bovis seu Bubali powder may further comprise the steps in the present embodiment: skeleton is pulverized, sterilization; Get Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and lactobacillus helveticus (L.helviticus), three kinds of lactobacillus weight ratios 3: 1: 2; It is 0.05% that aggregate and water w/v 1: 5, lactobacillus account for the aggregate weight ratio, adds 6% sucrose of gross weight, 50 ℃, and fermentation 16h, 120 ℃ of sterilization 30min; Add hydrochloric acid adjust pH 3, add pepsin, accounting for the aggregate weight ratio is 0.09%, and 40 ℃ of hydrolysis temperatures stir 30min, adds alkali and regulates pH value to 7, adds papain, and accounting for the aggregate weight ratio is 0.09%, 60 ℃, stirs 30min, the heating enzyme denaturing; Add 6% glucose of gross weight, add leuconostoc cremoris and citric acid, it is 0.06% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 20, fermentation temperature are 38 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of sterilizations are drying to obtain.
Embodiment 8: get 9 parts of calcium gluconate by weight, 20 parts of trehaloses, 10 parts of collagen polypeptides, mix homogeneously.Add adjuvant, make capsule.The molecular weight of collagen polypeptide is 3000～3800 dalton in the present embodiment.
Embodiment 9: get 12 parts of calcium carbonate by weight, 30 parts of trehaloses, 12 parts of collagen polypeptides, mix homogeneously.Add adjuvant, make tablet.The molecular weight of collagen polypeptide is 3800～4300 dalton in the present embodiment.
Embodiment 10: get 11 parts of enzymolysis Os Bovis seu Bubali powder by weight, 24 parts of trehaloses, 11 parts of collagen polypeptides, mix homogeneously.Add adjuvant, make capsule.The molecular weight of collagen polypeptide is 4300～5000 dalton in the present embodiment.
The preparation method of enzymolysis Os Bovis seu Bubali powder may further comprise the steps in the present embodiment: skeleton is pulverized, sterilization; Get Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and lactobacillus helveticus (L.helviticus), three kinds of lactobacillus weight ratios 3: 1: 2; It is 0.05% that aggregate and water w/v 1: 4, lactobacillus account for the aggregate weight ratio, adds 6% sucrose of gross weight, 48 ℃, and fermentation 16h, 120 ℃ of sterilization 30min; Add hydrochloric acid adjust pH 2, add pepsin, accounting for the aggregate weight ratio is 0.09%, and 39 ℃ of hydrolysis temperatures stir 30min, adds alkali and regulates pH value to 6, adds papain, and accounting for the aggregate weight ratio is 0.09%, 55 ℃, stirs 30min, the heating enzyme denaturing; Add 6% glucose of gross weight, add leuconostoc cremoris and citric acid, it is 0.05% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 18, fermentation temperature are 38 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of sterilizations are drying to obtain.
Embodiment 11: extracting lactic acid calcium is 9 parts by weight, 20 parts of trehaloses, 10 parts of collagen polypeptides, 6 parts of conjugated linoleic acids, mix homogeneously.Add adjuvant, make tablet.The molecular weight of collagen polypeptide is 3000～3800 dalton in the present embodiment.
Embodiment 12: get 12 parts of enzymolysis Os Bovis seu Bubali powder by weight, 30 parts of trehaloses, 12 parts of collagen polypeptides, 10 parts of conjugated linoleic acids, mix homogeneously.Add adjuvant, make capsule.The molecular weight of collagen polypeptide is 3800～4300 dalton in the present embodiment.
The preparation method of enzymolysis Os Bovis seu Bubali powder may further comprise the steps in the present embodiment: skeleton is pulverized, sterilization; Get Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and lactobacillus helveticus (L.helviticus), three kinds of lactobacillus weight ratios 3: 1: 2; It is 0.05% that aggregate and water w/v 1: 3, lactobacillus account for the aggregate weight ratio, adds 6% sucrose of gross weight, 45 ℃, and fermentation 16h, 120 ℃ of sterilization 30min; Add hydrochloric acid adjust pH 2, add pepsin, accounting for the aggregate weight ratio is 0.09%, and 37 ℃ of hydrolysis temperatures stir 30min, adds alkali and regulates pH value to 6, adds papain, and accounting for the aggregate weight ratio is 0.09%, 50 ℃, stirs 30min, the heating enzyme denaturing; Add 6% glucose of gross weight, add leuconostoc cremoris and citric acid, it is 0.04% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 15, fermentation temperature are 38 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of sterilizations are drying to obtain.
Embodiment 13: get 11 parts of calcium carbonate by weight, 24 parts of trehaloses, 11 parts of collagen polypeptides, 9 parts of conjugated linoleic acids, mix homogeneously.Add adjuvant, make tablet.The molecular weight of collagen polypeptide is 4300～5000 dalton in the present embodiment.
Embodiment 14: get 9 parts of calcium carbonate by weight, 20 parts of trehaloses, 10 parts of collagen polypeptides, 6 parts of conjugated linoleic acids, 4 parts of lysines, mix homogeneously.Add adjuvant, make capsule.The molecular weight of collagen polypeptide is 3000～3800 dalton in the present embodiment.
Embodiment 15: get 12 parts of enzymolysis Os Bovis seu Bubali powder by weight, 30 parts of trehaloses, 12 parts of collagen polypeptides, 10 parts of conjugated linoleic acids, 8 parts of lysines, mix homogeneously.Add adjuvant, make tablet.The molecular weight of collagen polypeptide is 3800～4300 dalton in the present embodiment.
The preparation method of enzymolysis Os Bovis seu Bubali powder may further comprise the steps in the present embodiment: skeleton is pulverized, sterilization; Get Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and lactobacillus helveticus (L.helviticus), three kinds of lactobacillus weight ratios 3: 1: 2; It is 0.05% that aggregate and water w/v 1: 5, lactobacillus account for the aggregate weight ratio, adds 6% sucrose of gross weight, 50 ℃, and fermentation 16h, 120 ℃ of sterilization 30min; Add hydrochloric acid adjust pH 3, add pepsin, accounting for the aggregate weight ratio is 0.09%, and 40 ℃ of hydrolysis temperatures stir 30min, adds alkali and regulates pH value to 7, adds papain, and accounting for the aggregate weight ratio is 0.09%, 60 ℃, stirs 30min, the heating enzyme denaturing; Add 6% glucose of gross weight, add leuconostoc cremoris and citric acid, it is 0.06% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 20, fermentation temperature are 38 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of sterilizations are drying to obtain.
Embodiment 16: get 11 parts of calcium gluconate by weight, 28 parts of trehaloses, 11 parts of collagen polypeptides, 7 parts of conjugated linoleic acids, 6 parts of lysines, mix all with.Add adjuvant, make capsule.The molecular weight of collagen polypeptide is 3000～3800 dalton in the present embodiment.
Embodiment 17: get 9 parts of enzymolysis Os Bovis seu Bubali powder by weight, 20 parts of trehaloses, 10 parts of collagen polypeptides, 6 parts of conjugated linoleic acids, 4 parts of lysines, 0.02 part in vitamin V B120.02 part and folic acid, mix homogeneously.Add adjuvant, make tablet.The molecular weight of collagen polypeptide is 3800～4300 dalton in the present embodiment.
The preparation method of enzymolysis Os Bovis seu Bubali powder may further comprise the steps in the present embodiment: skeleton is pulverized, sterilization; Get Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and lactobacillus helveticus (L.helviticus), three kinds of lactobacillus weight ratios 3: 1: 2; It is 0.05% that aggregate and water w/v 1: 4, lactobacillus account for the aggregate weight ratio, adds 6% sucrose of gross weight, 48 ℃, and fermentation 16h, 120 ℃ of sterilization 30min; Add hydrochloric acid adjust pH 2, add pepsin, accounting for the aggregate weight ratio is 0.09%, and 39 ℃ of hydrolysis temperatures stir 30min, adds alkali and regulates pH value to 6, adds papain, and accounting for the aggregate weight ratio is 0.09%, 55 ℃, stirs 30min, the heating enzyme denaturing; Add 6% glucose of gross weight, add leuconostoc cremoris and citric acid, it is 0.05% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 18, fermentation temperature are 38 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of sterilizations are drying to obtain.
Embodiment 18: get 12 parts of calcium carbonate by weight, 30 parts of trehaloses, 12 parts of collagen polypeptides, 10 parts of conjugated linoleic acids, 8 parts of lysines, vitamin V B120.01 part, mix homogeneously.Add adjuvant, make capsule.The molecular weight of collagen polypeptide is 4300～5000 dalton in the present embodiment.
Embodiment 19: extracting lactic acid calcium is 11 parts by weight, 28 parts of trehaloses, 11 parts of collagen polypeptides, 7 parts of conjugated linoleic acids, 6 parts of lysines, 0.01 part in vitamin V B120.02 part and folic acid, mix homogeneously.Add adjuvant, make tablet.The molecular weight of collagen polypeptide is 3000～3800 dalton in the present embodiment.
Embodiment 20: get 9 parts of calcium carbonate by weight, 20 parts of trehaloses, 10 parts of collagen polypeptides, 6 parts of conjugated linoleic acids, 4 parts of lysines, 0.01 part in folic acid, 0.01 part of copper, mix homogeneously.Add adjuvant, make tablet.The molecular weight of collagen polypeptide is 3800～4300 dalton in the present embodiment.
Embodiment 21: get 12 parts of enzymolysis Os Bovis seu Bubali powder by weight, 30 parts of trehaloses, 12 parts of collagen polypeptides, 10 parts of conjugated linoleic acids, 8 parts of lysines, 0.02 part in vitamin V B120.02 part and folic acid, 0.02 part on 0.02 part of copper and selenium, mix homogeneously.Add adjuvant, make capsule.The molecular weight of collagen polypeptide is 4300～5000 dalton in the present embodiment.
The preparation method of enzymolysis Os Bovis seu Bubali powder may further comprise the steps in the present embodiment: skeleton is pulverized, sterilization; Get Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and lactobacillus helveticus (L.helviticus), three kinds of lactobacillus weight ratios 3: 1: 2; It is 0.05% that aggregate and water w/v 1: 3, lactobacillus account for the aggregate weight ratio, adds 6% sucrose of gross weight, 45 ℃, and fermentation 16h, 120 ℃ of sterilization 30min; Add hydrochloric acid adjust pH 2, add pepsin, accounting for the aggregate weight ratio is 0.09%, and 37 ℃ of hydrolysis temperatures stir 30min, adds alkali and regulates pH value to 6, adds papain, and accounting for the aggregate weight ratio is 0.09%, 50 ℃, stirs 30min, the heating enzyme denaturing; Add 6% glucose of gross weight, add leuconostoc cremoris and citric acid, it is 0.04% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 15, fermentation temperature are 38 ℃, and fermentation time is 12h, add the alkali neutralization, 120 ℃ of sterilizations are drying to obtain.
Embodiment 22: extracting lactic acid calcium is 11 parts by weight, 28 parts of trehaloses, 11 parts of collagen polypeptides, 7 parts of conjugated linoleic acids, 6 parts of lysines, 0.02 part in vitamin V B120.01 part and folic acid, 0.02 part on selenium, mix homogeneously.Add adjuvant, make tablet.The molecular weight of collagen polypeptide is 3800～4300 dalton in the present embodiment.
Claims (10)
1. the osteoporotic Chinese medicine composition of treatment comprises following components in part by weight: 9～12 parts of calcium preparation, 20～30 parts of trehaloses.
2. compositions according to claim 1 is characterized in that, described calcium is the calcium salt that animal or plant extracts.
3. compositions according to claim 1 is characterized in that, described calcium is one or more in calcium gluconate, calcium carbonate, calcium lactate and the enzymolysis bone meal.
4. compositions according to claim 3 is characterized in that, described calcium is the enzymolysis Os Bovis seu Bubali powder.
5. compositions according to claim 4 is characterized in that, the preparation method of described enzymolysis Os Bovis seu Bubali powder comprises the steps: that skeleton is pulverized, sterilization; Get Bifidobacterium lactis (B.lactis), Lactobacillus bulgaricus (L.bulgaricus) and lactobacillus helveticus (L.helviticus), three kinds of lactobacillus weight ratios 3: 1: 2; Aggregate and water w/v 1: 3～5, it is 0.05% that lactobacillus accounts for the aggregate weight ratio, adds 6% sucrose of gross weight, 45～50 ℃, fermentation 16h, 120 ℃ of sterilization 30min; Add hydrochloric acid adjust pH 2～3, add pepsin, accounting for the aggregate weight ratio is 0.09%, 37～40 ℃ of hydrolysis temperatures, stir 30min, add alkali and regulate pH value to 6～7, add papain, accounting for the aggregate weight ratio is 0.09%, 50～60 ℃, stir 30min, the heating enzyme denaturing; 6% the glucose that adds gross weight, add leuconostoc cremoris and citric acid, it is 0.04～0.06% that leuconostoc cremoris accounts for the aggregate weight ratio, leuconostoc cremoris and citric acid weight ratio 1: 15～20, fermentation temperature is 38 ℃, and fermentation time is 12h, adds the alkali neutralization, 120 ℃ of sterilizations are drying to obtain.
6. according to the described compositions of the arbitrary claim of claim 1-5, it is characterized in that, also comprise weight portion in the described component and be 6～10 parts conjugated linoleic acid.
7. according to the described compositions of the arbitrary claim of claim 1-6, it is characterized in that, also comprise weight portion in the described component and be 10～12 parts collagen polypeptide.
8. according to the described compositions of the arbitrary claim of claim 1-7, it is characterized in that, also comprise weight portion in the described component and be 4～8 parts lysine.
9. according to the described compositions of the arbitrary claim of claim 1-8, it is characterized in that, also comprise weight portion in the described component and be 0.01～0.04 part vitamin and/or mineral; Described vitamin is VB12 and/or folic acid; Described mineral is copper and/or selenium.
10. according to the described compositions of the arbitrary claim of claim 7-9, it is characterized in that described collagen polypeptide molecular weight is 3000-5000 dalton.
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| CN108850421A (en) * | 2018-05-04 | 2018-11-23 | 天津天狮生物发展有限公司 | A kind of method and osteocalcin for extracting bone calcium |
| CN113797311A (en) * | 2021-09-22 | 2021-12-17 | 海南葫芦娃药业集团股份有限公司 | Multivitamin calcium zinc selenium granules for children and preparation method thereof |
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| EP1514540A1 (en) * | 2002-05-01 | 2005-03-16 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Calcium-containing tissue strengthening agents and use thereof |
| CN101268836A (en) * | 2007-03-23 | 2008-09-24 | 天津天狮生物工程有限公司 | Nutrition high calcium medicinal granule and preparing technique |
| CN102871123A (en) * | 2012-10-10 | 2013-01-16 | 天狮集团有限公司 | Method for preparing bone calcium and bone gla protein |
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| EP1514540A1 (en) * | 2002-05-01 | 2005-03-16 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Calcium-containing tissue strengthening agents and use thereof |
| CN101268836A (en) * | 2007-03-23 | 2008-09-24 | 天津天狮生物工程有限公司 | Nutrition high calcium medicinal granule and preparing technique |
| CN102871123A (en) * | 2012-10-10 | 2013-01-16 | 天狮集团有限公司 | Method for preparing bone calcium and bone gla protein |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108850421A (en) * | 2018-05-04 | 2018-11-23 | 天津天狮生物发展有限公司 | A kind of method and osteocalcin for extracting bone calcium |
| CN113797311A (en) * | 2021-09-22 | 2021-12-17 | 海南葫芦娃药业集团股份有限公司 | Multivitamin calcium zinc selenium granules for children and preparation method thereof |
| CN113797311B (en) * | 2021-09-22 | 2024-01-16 | 海南葫芦娃药业集团股份有限公司 | Multi-vitamin calcium zinc selenium granule for children and preparation method thereof |
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Application publication date: 20130724 |