CN103204964B - Porous magnetic carrier, preparation method and application to fixing of pollutant degrading bacteria - Google Patents
Porous magnetic carrier, preparation method and application to fixing of pollutant degrading bacteria Download PDFInfo
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Abstract
The invention discloses a porous magnetic carrier, a preparation method and application to fixing of pollutant degrading bacteria. The preparation method is as below: adding a polyethylene glycol 6000 solution into a three-necked bottle, heating, adding nanometer Fe3O4 magnetic particles, introducing N2, adding absolute alcohol, stirring, heating, dropwise adding styrene dissolved with benzoyl peroxide vinyl benzene, divinyl benzene, methyl acrylic acid, toluene and n-heptane, stirring, and extracting the microspheres with acetone as a solvent; and drying the microspheres after extraction to obtain the porous magnetic carrier. The porous magnetic carrier provided by the invention drives the bacteria to aggregate on carrier surface with magnetic particles as the core and in the internal holes; the bacteria is firmly retained in the carrier, so as to increase bacterial density and avoid the loss of bacteria; and as the magnetic carrier is first used in the bacterial solution for fixing efficient bacteria, the flora is the most closed to central magnetic nuclear, and is applied with the strongesy magnetic biological effect, thereby facilitating the improvement of biological metabolism activity and enhancement of competitiveness.
Description
Technical field
The invention belongs to technical field of biological sewage treatment, relate to the application of a kind of porous magnetic carrier and preparation method and fixed-contamination thing degradation bacteria.
Background technology
Immobilized microorganism technology, refer to and utilize measure that is chemical or physics, free microorganism is limited or is positioned within the scope of a certain particular space, retain the catalytic activity that it is intrinsic, be beneficial to the quantity improving microorganism in reactor, the processing power of raising system and adaptability, and the modern biotechnology that can be repeated and use continuously.In the application of this technology, the selection of immobilization carrier is very crucial.Along with the development of macromolecular material, many high-molecular gel carriers that is natural or synthetic are attempted in Microorganism incubation technology, and these materials have microorganism cells nontoxicity mostly, mass-transfer performance is good, stable in properties, has larger specific surface area, physical strength advantages of higher.But the microorganism being easy to run off just retains in the reactor by Microorganism incubation technology more, rely on the advantage on microbe population to reduce sludge loading, improve processing power, inherently do not improve activity and the metabolic capacity of microorganism self; And due to the susceptibility of microorganism, in fixation procedure, different carriers material and linking agent all can cause impact in various degree to microbic activity.
Along with microbiological development, investigator starts to be devoted to from varying environment, filter out the high efficient strain with various specific function, and prepare biotechnological formulation and be added in sewage-treating reactor, to improving the overall activity of mud and the processing power of system, this is referred to as microorganism enhanced technology.Since entering 21 century, the development of genetic engineering technique is maked rapid progress, and investigator can utilize the means such as DNA recombinates construct " superbacteria " and it be used in the biological reinforcing technology of sewage disposal.
No matter be screen the Black Liquor with Efficient Bacteria or artificial constructed genetic engineering bacterium that obtain, great majority wherein can show good contaminant removal capacity in laboratory environment, but when applying it in actual sewage treatment process, just many difficulties and obstacle can be run into, as microbial preparation runs off serious, need to add in a large number continuously; Not remarkable etc. to the raising effect of system processing power.This mainly due to, the microbial population cultivated in laboratory can not adapt to the new environment of actual treatment structures soon, and will and original " original inhabitants " microorganism between being at war with property grow, most of microbe preparation all can be in a disadvantageous position in this course, its metabolic capacity is subject to serious restriction, enough matrix can not be obtained to promote the breeding of its population, and be eliminated gradually or wither away.Wherein the latter is more basic and chief reason.
Early stage investigator finds that low-intensity magnetic field can produce positive magnetic biological effect to live microorganism and effectively improve microbial metabolic activity, accelerates the biochemical reaction rate of contaminant degradation, thus improves the processing efficiency of total system.But in application in the past, just add single magnetic, although the specific surface area of some magnetic is comparatively large, it can only competence exertion be to electronegative zoogleic adsorption in slant acidity environment, and its absorption property is more weak and can not fix efficiently and catalysis microorganism active; Particularly under long-term the action of a magnetic field, these magnetics can produce memory magnetic, are easy to reunite in mud, and lose its original effect.
Nano level magnetic particle has the responsiveness to externally-applied magnetic field, can be separated, but remove externally-applied magnetic field under outside magnetic field effect with liquid matrix, and magnetic particle can not be reunited because being magnetized, and without Magnetic Memory, can again be dispersed in liquid phase base fluid.These characteristics of magnetic particle overcome the deficiency of traditional magnetic in mud strengthening application just, show great using value.But magnetic particle is cubic crystal stone structure, smooth surface atresia, and its particle diameter too small cannot directly as carrier be used for microbial immobilization.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, a kind of porous magnetic carrier is provided.
Second object of the present invention is to provide a kind of preparation method of porous magnetic carrier.
3rd object of the present invention is to provide a kind of purposes of porous magnetic carrier.
Technical scheme of the present invention is summarized as follows:
A preparation method for porous magnetic carrier, is characterized in that comprising the steps:
Measure 50mL mass concentration be 7.50% the polyethylene glycol 6000 aqueous solution add in the three-necked bottle that prolong and whipping appts are housed, be heated to 60 DEG C, add the Fe that 12g particle diameter is 120-200nm
3o
4magnetic particle, passes into N
2add the dehydrated alcohol of 50mL, at remaining on 60 DEG C, stir 30min, increase the temperature to 75 DEG C, drip the 10mL vinylbenzene being dissolved with 2.50g benzoyl peroxide, drip 2mL Vinylstyrene, 1mL methacrylic acid again, 7.50mL toluene and 7.50mL normal heptane, under remaining on 400r/min, stir 3h, obtained microballoon is loaded in apparatus,Soxhlet's, with acetone as solvent, extracting 48h; Again the microballoon that extracting is good is put into baking oven, dry 24h at 60 DEG C, obtains porous magnetic carrier.
Porous magnetic carrier prepared by aforesaid method.
The application of porous magnetic carrier fixed-contamination thing degradation bacteria, comprises the steps:
(1) inoculate contaminant degradation bacterium in liquid medium within, add above-mentioned porous magnetic carrier and make the mass concentration of porous magnetic carrier be 0.5%, 30 DEG C of cultivations 72 hours;
(2) mixture that step (1) obtains is added in the bioreactor for disposing polluted water containing inoculation of activated-sludge.
Described contaminant degradation bacterium is preferably denitrogenation Bao Man Salmonella, bacillus cereus, Aeromonas or achromobacter.
Advantage of the present invention:
Experiment proves, porous magnetic carrier outward appearance prepared by method of the present invention is that black is granular, its surface microstructure defines a large amount of pore textures as seen, and aperture is distributed in the scope of less than 10 μm substantially, compares and is suitable for perching and growth and breeding of various microorganism.During for fixation of microbe, a large amount of microorganisms can be built up in its surface and hole.Thus, germs collect amount is large, growth and breeding fast, and being easy to material transfer and exchange, is good microbial fixed carrier.
Porous magnetic diameter of carrier (D [4,3]) is 196.90 μm, and specific surface area is 0.1709m
2/ g, wherein 50-500 μm of particle diameter proportion in overall product is about 80%, porous magnetic carrier in this particle size range all can meet the requirement of germs collect growth, therefore, after being screened out by micro-magnetic carrier of all the other particle diameters with the cell sieve that aperture is 50 μm and 500 μm again, it all can be used for the absorption of later stage efficient denitrification bacterium and fixing.
Magnetic responsiveness is the important indicator evaluating this porous magnetic carrier.The magnetic hysteresis loop of the carrier obtained with physical properties tester replication under different magnetic field intensity.Result is visible, and the coercive force of porous magnetic carrier is zero, and illustrate that it presents typical superparamagnetism feature, this also shows that magnetic microsphere belongs to ferromagnetic substance.
Measured by the X-ray diffraction of contrast magnetic particle and porous magnetic carrier, the magnetic fluid selected by us and and the porous magnetic carrier prepared, peak position does not change substantially, substantially assorted peak; Magnetic granule crystal belongs to isometric system spinel structure, characteristic diffraction peak clearly appear at 2 θ=18.3 °, 30.1 °, 35.4 °, 37.0 °, 43.1°、57。1°、62。6 ° and 74.1 ° of places, these diffraction peaks correspond respectively to the feature of each crystal face of magnetic particle.Show that the crystalline structure of magnetic particle in preparation process does not change, and agglomeration does not occur.And in the peak figure of porous magnetic carrier, except the characteristic diffraction peak same with magnetic Particle Phase, ° strong diffraction peak occurred in 2 θ=18.2 is then non-crystalline polystyrene diffraction peak.These results show in this preparation method, any chemical reaction is there is not between magnetic particle and polystyrene, it is inner that magnetic particle is just wrapped in polystyrene polymeric microballoon, and thus, prepared porous magnetic carrier still has good superparamagnetism.
Porous magnetic carrier of the present invention, at the microbial culture initial stage by the absorption on micro-magnetic carrier surface and fixed action, can order about bacterium and be easy to be gathered in the carrier surface and inner void that magnetic particle is core; It is retained in carrier comparatively securely, improves bacterial density simultaneously, avoid thalline and run off; Further, because magnetic carrier is first used to fix Black Liquor with Efficient Bacteria in bacterium liquid, therefore these flora distance center magnetic kernel nearests, suffered magnetic biological effect is the strongest, and it is active that this is conducive to improving its biological metabolism, strengthens its competitive capacity.
Accompanying drawing explanation
Fig. 1 is the surface tissue (magnification is 4000 times) of porous magnetic carrier.
Fig. 2 is the size distribution of porous magnetic carrier.
Fig. 3 is that under 300K, the magnetic hysteresis of micro-magnetic carrier stays loop line.
Fig. 4 is XRD spectra (a: porous magnetic carrier XRD spectra; B: magnetic fluid XRD spectra).
Fig. 5 is magnetic particle magnetic particle (magnetite, Fe+2Fe
2+ 3O
4) wide diffraction peak base peak figure.
Fig. 6 is the combination (magnification is 8000 times) of denitrogenation Bao Man Salmonella and porous magnetic carrier
Fig. 7 reactor process schematic flow sheet.
Fig. 8 two reactor compares organic removal effect
Fig. 9 two reactor is to NH
4 +the removal effect of-N compares
Denitrogenation Bao Man Salmonella and total bacterial 16 S rDNA copy number changes in Figure 10 two reactor
Embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated.
Embodiments of the invention understand the present invention better to enable those skilled in the art to, but do not impose any restrictions the present invention.
Embodiment 1
Material and equipment: vinylbenzene (St), benzoyl peroxide (BPO), methacrylic acid (MAA), Vinylstyrene (DVB), PEG-4000 (PEG), is top grade pure, dehydrated alcohol (analytical pure), purchased from Reagent Company of traditional Chinese medicines group, digital display constant speed stirrer, purchased from Community of Jin Tan County instrument plant.
Measure 50mL mass concentration be 7.50% the polyethylene glycol 6000 aqueous solution add in the three-necked bottle that prolong and whipping appts are housed, be heated to 60 DEG C, add the Fe that 12g median size is 150nm
3o
4magnetic particle, passes into N
2add the dehydrated alcohol of 50mL, at remaining on 60 DEG C, stir 30min, increase the temperature to 75 DEG C, drip the 10mL vinylbenzene being dissolved with 2.50g benzoyl peroxide, drip 2mL Vinylstyrene, 1mL methacrylic acid again, 7.50mL toluene and 7.50mL normal heptane, under remaining on 400r/min, stir 3h, obtained microballoon is loaded in apparatus,Soxhlet's, with acetone as solvent, extracting 48h; Again the microballoon that extracting is good is put into baking oven, dry 24h at 60 DEG C, obtains porous magnetic carrier.
Experiment proves: Fe
3o
4the median size of magnetic particle selects 120nm, 180nm or 200nm, other same the present embodiment, also can prepare corresponding porous magnetic carrier.
The sign of porous magnetic carrier prepared by above-described embodiment 1
Configuration of surface and structure: get porous magnetic carrier and be placed on anticreep slide, observe putting into field emission scanning electron microscope (FEI Nanosem430) after sample surfaces metal spraying with ion sputtering instrument.See Fig. 1
Porous magnetic diameter of carrier distributes: get the porous magnetic carrier 0.5g prepared and adopt laser particle size analyzer (Malvern Mastersizer, Malvern Instruments Ltd, UK) to measure its size distribution.See Fig. 2
Magnetic responsiveness and saturated magnetic intensity: get the porous magnetic carrier after 0.5g lyophilize, at 300k, be shown in Fig. 3 with physical properties tester (PPMS-9, Quantum Design Inc.USA)
Micro-magnetic carrier crystalline structure: get the magnetic fluid after preparing also lyophilize and each 0.5g of porous magnetic carrier, adopts x-ray powder diffraction instrument (D/MAX-2500, Japan) to analyze its crystalline structure; Optimum configurations is 2 θ, 5-90 °, 6 °/min.
In this research, at N
2under protection and necessarily stirring velocity, with ethanol/water mixed solution for dispersion medium, vinylbenzene is monomer; benzoyl peroxide is initiator, and polyoxyethylene glycol is stablizer, and Vinylstyrene is linking agent; with first benzene and heptane for pore-creating agent, prepare porous magnetic carrier.
The porous magnetic carrier outward appearance be prepared from is that black is granular, its surface microstructure as shown in Figure 1, as seen from Figure 1, a large amount of pore textures is defined at porous magnetic carrier surface, aperture is distributed in the scope of less than 10 μm substantially, compares and is suitable for perching and growth and breeding of various microorganism.During for fixation of microbe, a large amount of microorganisms can be built up in its surface and hole.Thus, germs collect amount is large, growth and breeding fast, and being easy to material transfer and exchange, is good microbial fixed carrier.
As shown in Figure 2, result shows porous magnetic diameter of carrier distribution range: obtained carrier median size (D [4,3]) is 196.90 μm, and specific surface area is 0.1709m
2/ g, wherein 50-500 μm of particle diameter proportion in overall product is about 80%, porous magnetic carrier in this particle size range all can meet the requirement of germs collect growth, therefore, after being screened out by micro-magnetic carrier of all the other particle diameters with the cell sieve that aperture is 50 μm and 500 μm again, it all can be used for the absorption of later stage efficient denitrification bacterium and fixing.
Magnetic responsiveness is the important indicator evaluating this porous magnetic carrier.The magnetic hysteresis loop of the carrier obtained with physical properties tester replication under different magnetic field intensity is as Fig. 3.Result is visible, and the coercive force of porous magnetic carrier is zero, and illustrate that it presents typical superparamagnetism feature, this also shows that magnetic microsphere belongs to ferromagnetic substance.
The X-ray diffraction measurement result of magnetic particle and porous magnetic carrier as shown in Figure 4.Scheme typical magnetic particle (magnetite, Fe+2Fe
2+ 3O
4) wide diffraction peak base peak figure.Visible by the contrast of Fig. 4 and Fig. 5, the magnetic fluid selected by us and and the porous magnetic carrier prepared, peak position does not change substantially, substantially assorted peak; Magnetic granule crystal belongs to isometric system spinel structure, characteristic diffraction peak clearly appear at 2 θ=18.3 °, 30.1 °, 35.4 °, 37.0 °, 43.1°、57。1°、62。6 ° and 74.1 ° of places, these diffraction peaks correspond respectively to the feature of each crystal face of magnetic particle.Show that the crystalline structure of magnetic particle in preparation process does not change, and agglomeration does not occur.
And in the peak figure of porous magnetic carrier, except the characteristic diffraction peak same with magnetic Particle Phase, ° strong diffraction peak occurred in 2 θ=18.2 is then non-crystalline polystyrene diffraction peak.These results show in this preparation technology, any chemical reaction is there is not between magnetic particle and polystyrene, it is inner that magnetic particle is just wrapped in polystyrene polymeric microballoon, and thus, prepared porous magnetic carrier still has good superparamagnetism.
Embodiment 2
The combination of porous magnetic carrier and denitrogenation Bao Man Salmonella (Bowmanella denitrificans, bacterial strain deposit number: MCCC1A00162)
Configure 2 bottles of 500mL liquid nutrient mediums.
Liquid nutrient medium: (NH4) 2SO40.25g, sodium succinate 1.08g, g, Vickers salts solution 25mL, adding distil water is dissolved to 500mL.
Wherein: Vickers salts solution: K
2hPO
45.0g, MgSO
47H
2o2.5g, NaCl2.5g, FeSO47H
2o0.05g, MnSO44H
2o0.05g, adds water after dissolving and is settled to 1000mL.
Wherein add porous magnetic carrier 2.5g prepared by embodiment 1 in the 1st bottle.Denitrogenation Bao Man Salmonella is inoculated respectively equally, 30 DEG C, 120r/min shaking table cultivation 72h in 2 bottle liquid nutrient mediums.
Getting cultivation back loading has the porous magnetic carrier of denitrogenation Bao Man Salmonella to be placed in 2.5% glutaraldehyde stationary liquid, and fix 12h at 4 DEG C, Gradient elution using ethanol is placed in field emission scanning electron microscope (FEI Nanosem430) and observes.Result as shown in Figure 6.Carrier inside hole and denitrogenation Bao Man Salmonella junction visible, a large amount of Bacterias is attached to growth and breeding in its hole.This also illustrates that this carrier is that the fixing of microorganism and growth provide excellent environment.
Embodiment 3
Practical application effect after porous magnetic carrier and denitrogenation Bao Man Salmonella (Bowmanella denitrificans, bacterial strain deposit number: MCCC1A00162) combine
(1) structure of reactor
Run parallel two sbr reactor devices, and lab scale sbr reactor device is made up of synthetic glass, height overall 0.6m, internal diameter 90mm, cumulative volume 3.8L, and total effectively body bulk is 3.5L.Experiment process schematic diagram as shown in Figure 7.In figure: 1 water inlet pipe, 2 vacuum breaker, 3 reactors, 4 liquidometers, 5 agitators, 6PLC controller, 7 electromagnetic field devices, 8 micro-hole aerators, 9 air flowmeter, 10 air delivering pipelines, 11 air compressor, 12 magnetic valves, 13 rising pipes, 14PLC control circuit.
COD in test
cr, NH
4 +-N analytical procedure choose main reference " water and waste water method for monitoring and analyzing " the 4th edition (State Environmental Protection Administration, 2002).
Sewage is intake through vacuum breaker 2 by the water inlet pipe 1 of reactor bottom, by reactor lower part rising pipe 13 water outlet.Water inlet pipe 1, rising pipe 13 start liquidometer 4 by PLC 6 by PLC control circuit 14 respectively and control water outlet solenoid valve 12 and operate.Aeration controls air compressor 11 by PLC 6, and gas under pressure delivers to the micro-hole aerator 8 in reactor 3 through the air flowmeter 9 be arranged on air delivering pipeline 10, from reactor bottom air feed, for system provides dissolved oxygen and hydraulic shear.Aeration rate is undertaken measuring and regulating by air flowmeter 9.Each cycle carrying out practically parameter is: water inlet 2min → anaerobism stirring 90min → aeration 240min → staticly settle 7min → water outlet 5min → idle 16min.6h is a cycle altogether, runs for 4 cycles every day.Seed sludge takes from certain sewage work's Aerobic Pond returned sluge, and intake as artificial distribution in laboratory, concrete moiety is in table 1.Volumetric loading is about 0.8kgTOC/ (m
3.d).Adopt the mode of micro-pore aeration to stir and oxygenation mud mixed liquid in bottom, aeration rate is 0.10 ~ 0.20m
3.h
-1.
Respectively to adding certain sewage work's Aerobic Pond returned sluge in two reactors, the sludge concentration after adding is 3.0g/L.And in 2 reactors, adding the above-mentioned 2 bottles of denitrogenation Bao Man Salmonellas cultivated respectively, the reactor containing porous magnetic carrier is labeled as SBR-M, and another reactor is labeled as SBR-S.
The main ingredient of table 1 artificial distribution and concentration (mg.L-1)
(2) reactor run duration is to the monitoring of denitrogenation Bao Man Salmonella number change
From reactor, sample 0.5mL mud mixed liquid at every turn, adopt OMEGA company E.Z.N.A.
tMsoil DNAKit extracts genomic dna in mud, and through electrophoresis detection, gained DNA segment equals or slightly larger than 23kb; Detect through spectrophotometer, its A260/A280, all 1.76 ~ 1.79, shows to obtain comparatively complete and that purity is higher genomic dna.Real-time quantitative PCR amplification can be carried out to total bacterium and denitrogenation Bao Man Salmonella respectively as template.Select Takara company
premix Ex Taq
tM(DRR041A) test kit, instrument is AB7300 real-time fluorescence quantitative PCR instrument.
(3) result and discussion
Two reactors to organic removal effect as shown in Figure 8.As can be seen, at reactor initial operating stage, M reactor is better than S reactor to the removal effect of CODCr, namely M reactor reached 75% to organic clearance at the 3rd day, and S reactor only has 22%, removal effect difference is so big, and this is mainly because 2 factors cause: the porous magnetic carrier 1. added is to the hormesis of microbial metabolism function; 2. the system cloud gray model initial stage, the vesicular structure of magnetic carrier is not yet wrapped up completely by mud, can produce certain adsorption effect, reduce the amount of Organic substance in water to the organism in water.The 9th day time, SBR-M reaches 90% to organic clearance, and keeps comparatively stable removal effect subsequently always.And SBR-S is the 14th day time, COD
crclearance also reach more than 90%.And at reactor run duration subsequently, two reactors no longer include notable difference to organic removal effect.Can be known by contrast, porous magnetic carrier add the acclimation period shortening mud in reactor assembly, the treatment effect started fast can be played.
Two reactors are to NH
4 +the removal effect of-N as shown in Figure 9.As can be seen, after reactor brings into operation, owing to having added efficient denitrification bacterium denitrogenation Bao Man Salmonella, therefore two reactors all show higher NH
4 +-N removal efficiency.But along with the extension of reactor working time, SBR-S after the 7th day to NH
4 +the place to go effect of-N starts to reduce, and reaches stable after the 16th day, now at water inlet NH
4 +-N concentration is under the condition of 40-45mg/L, its water outlet NH
4 +-N concentration is in the scope of 12-15mg/L.And what form with it sharp contrast is that SBR-M remains the NH of efficient stable at duration of test always
4 +-N removal efficiency, water outlet NH
4 +-N concentration remains on 3-5mg/L.
Add micro-magnetic carrier to serve act on more significantly the denitrification ability improving the reactor start-up domestication stage, shorten the acclimation period of reactor assembly degradation of ammonia nitrogen significantly, make it reach to remove with COD the quick startup effect that course synchronization carries out.
In two reactors, the Quantitative Monitoring result of denitrogenation Bao Man Salmonella as shown in Figure 10.Along with the operation of reactor, in SBR-S, the loss of denitrogenation Bao Man Salmonella is serious, and its copy number is from 3.19 × 10 of initial operating stage
11copies. μ L
-1, reduce gradually, the 69th day time, only have 3.50 × 10
6copies. μ L
-1; And the quantity of denitrogenation Bao Man Salmonella remains on the comparatively stable order of magnitude in SBR-M always.This also with two reactors to NH
4 +the variation tendency of the removal efficiency of-N is consistent.It can thus be appreciated that, porous magnetic carrier prepared by the inventive method is used for the fixing of denitrogenation Bao Man Salmonella, effectively can strengthen competition and the metabolic capacity of added microbial inoculum, prevent the loss of the denitrogenation Bao Man Salmonella added in Sewage treatment systems, improve and keep the efficient denitrification ability of reactor assembly, considerably reducing and continue to add to maintain higher processing efficiency the high cost of sewage disposal that microbial preparation produces.
Through experiment, bacillus cereus (Bacillus cereus), preserving number SCTCC100857; Bacillus cereus (Bacillus cereus), preserving number SCTCC100858; Aeromonas (sp.Aeromonas), preserving number CICC23566; Achromobacter (sp.Leucobacter), the porous magnetic carrier that preserving number CICC10313 all can be prepared with embodiment 1 is respectively combined, and after testing, a large amount of bacterial adhesion is growth and breeding in porous magnetic carrier inside hole.
The porous magnetic carrier of each bacterium above-mentioned will be combined, the porous magnetic carrier combining denitrogenation Bao Man Salmonella of alternate embodiment 3 respectively, the other the same as in Example 3, experimental result shows, each porous magnetic carrier combining above-mentioned bacterium all effectively strengthen add competition and the metabolic capacity of microbial inoculum, prevent the loss of the bacterium added in Sewage treatment systems, improve and keep the efficient denitrification ability of reactor assembly, considerably reducing and continue to add to maintain higher processing efficiency the high cost of sewage disposal that microbial preparation produces.
Claims (4)
1. a preparation method for porous magnetic carrier, is characterized in that comprising the steps:
Measure 50mL mass concentration be 7.50% the polyethylene glycol 6000 aqueous solution add in the three-necked bottle that prolong and whipping appts are housed, be heated to 60 DEG C, add the Fe that 12g particle diameter is 120-200nm
3o
4magnetic particle, passes into N
2add the dehydrated alcohol of 50mL, stir 30min at remaining on 60 DEG C, increase the temperature to 75 DEG C, drip the 10mL vinylbenzene, 2mL Vinylstyrene, the 1mL methacrylic acid that are dissolved with 2.50g benzoyl peroxide, 7.50mL toluene and 7.50mL normal heptane, under remaining on 400r/min, stir 3h, obtained microballoon is loaded in apparatus,Soxhlet's, with acetone as solvent, extracting 48h; Again the microballoon that extracting is good is put into baking oven, dry 24h at 60 DEG C, obtains porous magnetic carrier.
2. the porous magnetic carrier prepared of the method for claim 1.
3. the application of the porous magnetic carrier fixed-contamination thing degradation bacteria of claim 2, is characterized in that comprising the steps:
(1) inoculate contaminant degradation bacterium in liquid medium within, the porous magnetic carrier adding claim 2 makes the mass concentration of porous magnetic carrier be 0.5%, 30 DEG C of cultivations 72 hours;
(2) mixture that step (1) obtains is added in the bioreactor for disposing polluted water containing inoculation of activated-sludge.
4. application according to claim 3, is characterized in that described contaminant degradation bacterium is denitrogenation Bao Man Salmonella, bacillus cereus, Aeromonas or achromobacter.
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| CN105565506B (en) * | 2015-12-17 | 2018-03-30 | 苏州大学 | It is a kind of to load Biocomposite material of magnetic nanoparticle with core shell structure and its production and use |
| CN108385116B (en) * | 2018-02-10 | 2019-07-12 | 吉林省聚德管道有限公司 | A kind of acid-washing stainless steel passivating solution and preparation method thereof |
| CN108751397A (en) * | 2018-05-31 | 2018-11-06 | 北京北华中清环境工程技术有限公司 | Add the method that functionalized magnetic microsphere carries out coal gas wastewater processing using MBR |
| CN110241110B (en) * | 2019-06-14 | 2021-04-27 | 浙江科技学院 | Method for immobilizing dibutyl phthalate degrading bacteria by using magnetic nanoparticles and application |
| CN113122468B (en) * | 2019-12-30 | 2023-03-10 | 杭州远大生物制药有限公司 | Method for preparing bacillus cereus and application thereof |
| CN111298730B (en) * | 2020-03-02 | 2021-09-24 | 江苏科技大学 | Magnetic biological microcapsule, preparation method and application thereof |
| CN115531516A (en) * | 2022-10-11 | 2022-12-30 | 杭州师范大学 | Recyclable magnetic mesoporous antibacterial peptide nanoparticles constructed based on Fe-S bonds and preparation method and application thereof |
| CN116121145A (en) * | 2023-02-27 | 2023-05-16 | 中国科学院海洋研究所 | Bowman's denitrificans and application thereof |
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