CN106006950A - Method for promoting granulation of aerobic sludge through mycelium pellets - Google Patents
Method for promoting granulation of aerobic sludge through mycelium pellets Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1236—Particular type of activated sludge installations
- C02F3/1263—Sequencing batch reactors [SBR]
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- C—CHEMISTRY; METALLURGY
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- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention provides a method for promoting granulation of aerobic sludge through mycelium pellets and relates to environment bioengineering. The method includes the following steps that chlorophenol model wastewater is prepared; the mycelium pellets are prepared; the mycelium pellets are put into an SBR; the reactor is started and aerobic granular sludge is cultured; in the process that the reactor is started and runs, the particle size, appearance, settlement performance, density, water content, porosity and other properties of the aerobic granular sludge are periodically observed, quality of outflow water is periodically detected, and the sludge granulation degree, the stability degree of granular sludge long-time operation and the chlorophenol model wastewater removal effect are analyzed and judged. The culture period of sludge granulation can be greatly shortened, long-time operation stability of sludge particles in the reactor is improved, and organic waste treatment efficiency is enhanced.
Description
Technical field
The present invention relates to environment bioengineering, particularly relate to a kind of method that mycelium pellet promotes aerobic sludge granulation.
Background technology
Aerobic particle mud (aerobic granular sludge, AGS) be microorganism under suitable environmental condition, a kind of granular agglomerate activated sludge formed by spontaneous cohesion.Compared with ordinary activated sludge, aerobic particle mud has that structure is closely knit, settling property good, biofacies is high, metabolic activity is good, capacity of resisting impact load is strong, be not susceptible to sludge bulking and remove the advantages, the most extremely extensive concern of lot of domestic and foreign scholar such as poisonous and harmful substance is effective.But, granule sludge cultivation cycle is the most long, from sludge acclimatization to particle maturation, mostly need to experience the time of 1～about 2 months, and the granule sludge after maturation is unable to maintain that operation steady in a long-term, the phenomenons such as disintegration, settling property variation easily occur, thus limit aerobic particle mud popularization in actual industrial waste water treatment applications and industrialization.Therefore, the most quickly realize granular sludge, to maintain long-time steady operation be to make aerobic particle mud large-scale promotion and apply key issue urgently to be resolved hurrily in Practical Project.
Add molecule to realize the fast culture of aerobic particle mud as nucleus being the more effective solution of one by outside.The formation mechenism of this method is based on induced nuclei hypothesis, it considers that the formation of aerobic particle mud is the process of a kind of similar crystallization, first have to a nucleus as germs collect and the place of apposition growth, microorganism is adhered on nucleus, constantly assemble growth, particle diameter becomes larger, until forming ripe granule sludge.The existence of induced nuclei can accelerate the granular progress of sludge aerobic, saves the short grained formation time.Common adds inorganic particulate matter and the aerobic/anaerobic granule sludges etc. such as the active charcoal of nucleus granule, Calx, crystal micro-powder.But, utilize mycelium pellet to promote that the correlational study of aerobic sludge granulation there is not yet report the most both at home and abroad as nucleus.
Mycelium pellet be by mycete or actinomycetes under the conditions of liquid floods shaken cultivation, automatically assemble the one formed both there is vital signs, have again the spherical particle thing of absorption and carrier characteristics.Due to the ingredient of mycelium pellet inherently microbial cells, there is preferable hydrophobe equilibrium valve；And electrostatic attraction, functional group or the elements etc. that the extracellular polymeric of mycelia secretion and surface thereof exist all are conducive to the adhesion to other microbial cells, its distinctive meshy surface structure is also beneficial to perching and breeding of microorganism, is thus advantageous to mud attachment fixing formation mud granule.Analyze theoretically, mycelium pellet is added in activated sludge, induced nuclei can be formed, space surface is provided for the mutual Absorption Growth of microorganism, promote that microbial assembles formation granule sludge mutually, and the physical strength of mud granule will be greatly increased, show superior physical characteristic.
Therefore, the present invention based on aerobic particle mud " induced nuclei hypothesis ", add mycelium pellet as induced nuclei, accelerate the granulating of aerobic sludge, to playing positive impetus for the Aerobic Granular Sludge Technology actual application in Industrial Wastewater Treatment.
Summary of the invention
It is an object of the invention to provide a kind of method that mycelium pellet promotes aerobic sludge granulation, the method, using mycelium pellet as induced nuclei, utilizes its special construction that aerobic activated sludge attachment is fixed on hyphal surface, reaches to accelerate the purpose of aerobic sludge granulation.The mud granule structure formed is closely knit, settling property is good; physical strength is high; capacity of resisting impact load is strong; not only solve the problems such as sludge settling speed is slow, sludge concentration is low in conventional SBR reactor; and the cultivation cycle of granular sludge can be greatly shortened; improve mud granule longtime running stability in the reactor, strengthen treatment of Organic Wastewater efficiency.
It is an object of the invention to be achieved through the following technical solutions:
The present invention proposes one and utilizes mycelium pellet as induced nuclei, the method promoting aerobic sludge granulation.Concrete steps include:
1, preparation chlorophenol simulated wastewater
With the tap water preparation simulated wastewater containing 2-chlorophenol, its component includes: 0.5 ~ 2 grams per liter glucose, 0.1 ~ 1.0 grams per liter (NH4) 2SO4,0.05 ~ 0.5 grams per liter MgSO4 7H2O, 0.05 ~ 0.2 grams per liter KH2PO4,0.05 ~ 0.2 grams per liter K2HPO4,0.01 ~ 0.05 grams per liter CaSO4,50 ~ 150 mg/litre 2-chlorophenols, pH is nature.Now, inlet COD concentration is about 500 ~ 2500 mg/litre.
2, mycelium pellet is prepared
On aseptic operating platform, Sterile pipette is used to pipette 10 milliliters of fungal spore suspensions (containing about 103 ~ 1010, spore in every ml deionized water) in the conical flask equipped with 90 milliliters of aseptic chlorophenol simulated wastewaters.The sealing of conical flask sterile gauze being placed in air bath agitator and carry out shaken cultivation, rotating speed is 100 ~ 200 revs/min, and cultivation temperature is 25 ~ 35 DEG C.After cultivating the mycelium pellet forming size 1 ~ 3 millimeter uniform, a diameter of, taking-up sterile gauze filters, and cleans 3 times with physiological saline solution, is then immersed in physiological saline solution standby.
3, mycelium pellet adding in sbr reactor device
Test uses sequencing batch activated sludge reactor (Sequencing Batch Reactor, SBR) technique.Taking the aerobic activated sludge that sludge concentration (MLSS) is 1000 ~ 5000 mg/litre to be added in sbr reactor device, without any nutrient substance, continuous aeration stood 30 minutes after 48 hours, poured out supernatant.Mycelium pellet is added in sbr reactor device, by chlorophenol simulated wastewater injecting reactor after starting to run continuously by dosing ratio (mycelium pellet dry weight and the percentage ratio of activated sludge dry weight) 5% ~ 30%.
4, the startup of reactor and the cultivation of aerobic particle mud
Forming a cycle of operation from water inlet for the first time to second time water inlet, design cycle of operation number every day is 2 ~ 8,3 ~ 12 hours each cycle of operation time.Wherein, within each cycle, flooding time is set as 2 ~ 20 minutes, and aeration time is 120 ~ 600 minutes, and the sedimentation time is 2～20 minutes, standby time 5～120 minutes, water discharge time is 2 ~ 30 minutes, and aeration rate is 0.1 ~ 0.5 cube m/h, and oxygen in water is 2 ~ 5 mg/litre, hydraulic detention time 2 ~ 12 hours, water conservancy shearing force 0.5 ~ 3.0 cel.Volume-exchange rate 30% ~ 80%.
In reactor start-up running; routine observation aerobic particle mud particle diameter, mode of appearance, settling property, density and the character such as moisture content, porosity; and results of regular determination effluent quality, analyze and judge granular sludge degree, the degree of stability of granule sludge longtime running and the removal effect of parachlorophenol simulated wastewater.
Advantages of the present invention with effect is:
The present invention, using mycelium pellet as induced nuclei, utilizes its special construction that aerobic activated sludge attachment is fixed on hyphal surface, thus reaches to accelerate the purpose of aerobic sludge granulation.The mud granule structure formed is closely knit, settling property is good; physical strength is high; capacity of resisting impact load is strong; not only solve the problems such as sludge settling speed is slow, sludge concentration is low in conventional SBR reactor; and the cultivation cycle of granular sludge can be greatly shortened; improve mud granule longtime running stability in the reactor, strengthen treatment of Organic Wastewater efficiency.In addition; the method also has the advantages such as easy and simple to handle, production cost is low, the repeatable utilization of mycelium pellet; for development, this promotes that aerobic granular technology provides new technical method, the most also moved towards actual industrial waste water treatment applications for the aerobic granule technology of solution by laboratory and provide technical support and test basis.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of sequencing batch activated sludge reactor；
Fig. 2 is mycelium pellet outward appearance electromicroscopic photograph (40 ×)；
Fig. 3 is mycelium pellet induced synthesis aerobic particle mud outward appearance stereoscan photograph (40 ×).
In figure: 1. inlet water tank；2. immersible pump；3. dissolved oxygen meter；4. glass rotameter；5. air compressor machine；6. core aeration head；7. drain solenoid valve；8. the time relay；9. discharge outlet；10. sample tap；11. blow-down pipes.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in detail.
Photosynthetic bacteria provided by the present invention is the Rhodopseudomonas palustris (Rhodopseudomonas palustris) of a strain degradable 2-chlorophenol, temporary named PSB-1D, and 16SrDNA sequence is GenBank Accession No. HM068966.This bacterium is known existing strain, all has preservation in each big culture presevation storehouse.
The Phanerochaete chrysosporium (Phanerochaete chrysosporium) that microorganism is a named DH1 of strain forming mycelium pellet provided by the present invention.This bacterium is known existing strain, all has preservation in each big culture presevation storehouse.
The present invention uses HZQ-C type air bath oscillator as microbial cultivation equipment.
The high pressure steam sterilization of the present invention uses LD2X-40BI electric heating pressure steam sterilizer.
Sterile deionized water, normal saline that the present invention uses process through high pressure steam sterilization, and treatment conditions are temperature 121 DEG C, and pressure 0.105 MPa maintains 20 ~ 30 minutes.
Embodiment 1:
1, preparation chlorophenol simulated wastewater
With the tap water preparation simulated wastewater containing 2-chlorophenol, its component includes: 0.5 grams per liter glucose, 0.1 grams per liter (NH4) 2SO4,0.05 grams per liter MgSO4 7H2O, 0.062 grams per liter KH2PO4,0.062 grams per liter K2HPO4,0.012 grams per liter CaSO4,50 mg/litre 2-chlorophenols, pH is nature.Now, inlet COD concentration is about 550 mg/litre.
2, mycelium pellet is prepared
On aseptic operating platform, Sterile pipette is used to pipette 10 milliliters of fungal spore suspensions (containing about 105, spore in every ml deionized water) in the conical flask equipped with 90 milliliters of aseptic chlorophenol simulated wastewaters.The sealing of conical flask sterile gauze being placed in air bath agitator and carry out shaken cultivation, rotating speed is 160 revs/min, and cultivation temperature is 30 DEG C.After cultivating the mycelium pellet of size to be formed 2 millimeter uniform, a diameter of, taking-up sterile gauze filters, and cleans 3 times with physiological saline solution, is then immersed in physiological saline solution standby.
3, mycelium pellet is added to sbr reactor device
The SBR bioreactor that test uses device to be designed, designed.The main body of this reactor is cylindrical plexiglass box, and total height is 100 centimetres, and internal diameter is 9 centimetres, and dischargeable capacity is 5 liters.3 drainings/water intaking valve is set along sidewall.After starting to run continuously, through ZQB4X5-24 type immersible pump 2, the chlorophenol simulated wastewater in inlet water tank 1 is injected in reactor, in reactor, the oxygen needed for mud is provided by ACO-318 type air compressor 5, aeration is carried out by the core aeration head 6 of reactor bottom, and regulated aeration rate by LZB-3 type glass rotameter 4, utilize RSS-5100 type portable dissolved oxygen analytic instrument 3 on-line determination dissolved oxygen concentration DO value, ZDT-B1 drain solenoid valve 7 control draining.Whole service system use the time relay 8 control water inlet, aeration, precipitate, leave unused, the time in draining each stage.
Taking the aerobic activated sludge that sludge concentration (MLSS) is 3850 mg/litre to be added in sbr reactor device, without any nutrient substance, continuous aeration stood 30 minutes after 48 hours, poured out supernatant.Mycelium pellet is added in sbr reactor device, by 5 liters of chlorophenol simulated wastewater injecting reactors after starting to run continuously by dosing ratio (mycelium pellet dry weight and the percentage ratio of activated sludge dry weight) 15%.
4, the startup of sbr reactor device
Forming a cycle of operation from water inlet for the first time to second time water inlet, design cycle of operation number every day is 6,4 hours each cycle of operation time.Wherein, within each cycle, flooding time is set as 10 minutes, and aeration time is 200 minutes, sedimentation time is 5 minutes, 20 minutes standby time, water discharge time is 5 minutes, and displacement is 60 ls/h, aeration rate is 0.2 cube m/h, hydraulic detention time 6 hours, water conservancy shearing force is 1.2 cels, volume-exchange rate 60%.
Under above-mentioned entry condition, adding mycelium pellet and run after 13 days as the reactor of induced nuclei and realize complete granular sludge, compared with the aerobic particle mud of preparation conventional with under identical entry condition, incubation time shortens 38 days.The granular sledge size distribution of mycelium pellet induced synthesis is uniform, and structure is closely knit, regular shape; particle diameter is stably held in 3 ~ 5 millimeters, and porosity is 0.60 ~ 0.88, and density is 1.216 ~ 1.247 grams per liters; sludge settling speed is 32.82 ms/h, and SVI is 26 mls/g.Granule sludge in the reactor can long term maintenance stable operation 120 days, period integrity factor is consistently greater than more than 95%, expansion or the disintegration of granule sludge do not occur, each cycle to the removal of 2-chlorophenol in simulated wastewater all the time more than 82%, COD removes more than 90%, organic loading is about 3.5 kilograms per cubic meter skies, significantly better than under identical service condition, (2-chlorophenol clearance is about 70% to the conventional treatment effect preparing aerobic granular sludge reactor, COD clearance is about 85%, and organic loading is about 2.8 kilograms per cubic meter skies).
Embodiment 2:
1, preparation simulation Chlorophenol
With the tap water preparation simulated wastewater containing 2-chlorophenol, its component includes: 1.5 grams per liter glucoses, 0.5 grams per liter (NH4) 2SO4,0.05 grams per liter MgSO4 7H2O, 0.01 grams per liter KH2PO4,0.01 grams per liter K2HPO4,0.02 grams per liter CaSO4,100 mg/litre 2-chlorophenols, pH is nature.Now, inlet COD concentration about 1700 mg/litre.
2, preparation mixed vaccine pompon
On aseptic operating platform, Sterile pipette is used to inoculate in the 10 milliliters of fungal spore suspensions (containing about 107, spore in every ml deionized water) simulation Chlorophenol after 90 milliliters of sterilizing coolings.After conical flask being sealed with sterile gauze, being placed in air bath agitator and carry out shaken cultivation, rotating speed is 180 revs/min, and cultivation temperature is 28 DEG C.After cultivating the mycelium pellet of size to be formed 1 millimeter uniform, a diameter of, taking-up sterile gauze filters, and after cleaning 3 times with physiological saline solution, is immersed in physiological saline solution standby.
3, mycelium pellet is added to sbr reactor device
The SBR bioreactor that test uses device to be designed, designed.The main body of this reactor is cylindrical plexiglass box, and total height is 100 centimetres, and internal diameter is 9 centimetres, and dischargeable capacity is 5 liters.3 drainings/water intaking valve is set along sidewall.After starting to run continuously, through ZQB4X5-24 type immersible pump 2, the chlorophenol simulated wastewater in inlet water tank 1 is injected in reactor, in reactor, the oxygen needed for mud is provided by ACO-318 type air compressor 5, aeration is carried out by the core aeration head 6 of reactor bottom, and regulated aeration rate by LZB-3 type glass rotameter 4, utilize RSS-5100 type portable dissolved oxygen analytic instrument 3 on-line determination dissolved oxygen concentration DO value, ZDT-B1 drain solenoid valve 7 control draining.Whole service system use the time relay 8 control water inlet, aeration, precipitate, leave unused, the time in draining each stage.
Taking the aerobic activated sludge that sludge concentration (MLSS) is 3270 mg/litre to be added in sbr reactor device, without any nutrient substance, continuous aeration stood 30 minutes after 48 hours, poured out supernatant.Mycelium pellet is added in sbr reactor device, by 5 liters of chlorophenol simulated wastewater injecting reactors after starting to run continuously by dosing ratio (mycelium pellet dry weight and the percentage ratio of activated sludge dry weight) 25%.
4, the startup of sbr reactor device
Forming a cycle of operation from water inlet for the first time to second time water inlet, design cycle of operation number every day is 4,6 hours each cycle of operation time.Wherein, within each cycle, flooding time is set as 5 minutes, and aeration time is 320 minutes, sedimentation time is 5 minutes, 20 minutes standby time, water discharge time is 10 minutes, and displacement is 24 ls/h, aeration rate is 0.15 cube m/h, volume-exchange rate 80%, hydraulic detention time 7.5 hours, water conservancy shearing force is 1.2 cels.
Under above-mentioned entry condition, adding mycelium pellet and run after 16 days as the reactor of induced nuclei and realize complete granular sludge, compared with the aerobic particle mud of preparation conventional with under identical entry condition, incubation time shortens 35 days.The granular sledge size distribution of mycelium pellet induced synthesis is uniform, and structure is closely knit, regular shape; particle diameter is stably held in 2 ~ 4 millimeters, and porosity is 0.76 ~ 0.95, and density is 1.106 ~ 1.231 grams per liters; sludge settling speed is 34.65 ms/h, and SVI is 28 mls/g.Granule sludge in the reactor can long term maintenance stable operation 105 days, period integrity factor is consistently greater than more than 95%, expansion or the disintegration of granule sludge do not occur, each cycle to the removal of 2-chlorophenol in simulated wastewater all the time more than 86%, COD removes more than 92%, organic loading is about 3.8 kilograms per cubic meter skies, significantly better than under identical service condition, (2-chlorophenol clearance is about 80% to the conventional treatment effect preparing aerobic granular sludge reactor, COD clearance is about 88%, and organic loading is about 3.2 kilograms per cubic meter skies).
Claims (2)
1. the method that a mycelium pellet promotes aerobic sludge granulation, it is characterised in that described method includes procedure below:
1) preparation chlorophenol simulated wastewater
With the tap water preparation simulated wastewater containing 2-chlorophenol, its component includes: 0.5 ~ 2 grams per liter glucose, 0.1 ~ 1.0 grams per liter (NH4)2SO4, 0.05 ~ 0.5 grams per liter MgSO4·7H2O, 0.05 ~ 0.2 grams per liter KH2PO4, 0.05 ~ 0.2 grams per liter K2HPO4, 0.01 ~ 0.05 grams per liter CaSO4, 50 ~ 150 mg/litre 2-chlorophenols, pH is nature；Now, inlet COD concentration is 500 ~ 2500 mg/litre；
2) mycelium pellet is prepared
On aseptic operating platform, Sterile pipette is used to pipette 10 milliliters of fungal spore suspensions, in the conical flask equipped with 90 milliliters of aseptic chlorophenol simulated wastewaters；The sealing of conical flask sterile gauze being placed in air bath agitator and carry out shaken cultivation, rotating speed is 100 ~ 200 revs/min, and cultivation temperature is 25 ~ 35 DEG C；After cultivating the mycelium pellet forming size 1 ~ 3 millimeter uniform, a diameter of, taking-up sterile gauze filters, and cleans 3 times with physiological saline solution, is then immersed in physiological saline solution standby；
3) mycelium pellet adding in sbr reactor device
Test uses sequencing batch activated sludge reactor (Sequencing Batch Reactor, SBR) technique；Taking the aerobic activated sludge that sludge concentration (MLSS) is 1000 ~ 5000 mg/litre to be added in sbr reactor device, without any nutrient substance, continuous aeration stood 30 minutes after 48 hours, poured out supernatant；Mycelium pellet is pressed dosing ratio, i.e. the percentage ratio 5% ~ 30% of mycelium pellet dry weight and activated sludge dry weight adds in sbr reactor device, after starting to run continuously, chlorophenol simulated wastewater injecting reactor is joined；
4) startup of reactor and the cultivation of aerobic particle mud
Forming a cycle of operation from water inlet for the first time to second time water inlet, design cycle of operation number every day is 2 ~ 8,3 ~ 12 hours each cycle of operation time；Wherein, within each cycle, flooding time is set as 2 ~ 20 minutes, and aeration time is 120 ~ 600 minutes, and the sedimentation time is 2～20 minutes, standby time 5～120 minutes, water discharge time is 2 ~ 30 minutes, and aeration rate is 0.1 ~ 0.5 cube m/h, and oxygen in water is 2 ~ 5 mg/litre, hydraulic detention time 2 ~ 12 hours, water conservancy shearing force 0.5 ~ 3.0 cel；Volume-exchange rate 30% ~ 80%；
In reactor start-up running; routine observation aerobic particle mud particle diameter, mode of appearance, settling property, density and the character such as moisture content, porosity; and results of regular determination effluent quality, analyze and judge granular sludge degree, the degree of stability of granule sludge longtime running and the removal effect of parachlorophenol simulated wastewater.
A kind of mycelium pellet the most according to claim 1 promotes the method for aerobic sludge granulation, it is characterised in that containing spore about 10 in the described every ml deionized water of fungal spore suspension3~1010Individual.
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| US12049418B2 (en) * | 2021-10-09 | 2024-07-30 | Hunan Sanyou Environmental Protection Technology Co. LTD | Sewage treatment biological agent and preparation method and application thereof |
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