CN105441364A - Microorganisms and method for rapid start of aniline wastewater - Google Patents
Microorganisms and method for rapid start of aniline wastewater Download PDFInfo
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- CN105441364A CN105441364A CN201511021930.6A CN201511021930A CN105441364A CN 105441364 A CN105441364 A CN 105441364A CN 201511021930 A CN201511021930 A CN 201511021930A CN 105441364 A CN105441364 A CN 105441364A
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Abstract
The invention relates to microorganisms and a method for rapid start of aniline wastewater and aims to solve the problems of slower start of the aniline wastewater and unstable treatment effect at present. The microorganisms comprise aniline degradation bacteria, rapid COD (chemical oxygen demand) degradation bacteria, activated sludge, nutrients and tourmaline. The method for rapid start of the aniline wastewater comprises the following steps: 1, acclimating sludge to obtain sludge with high aniline degradation activity; 2, filling an aniline wastewater treatment bioreactor with the aniline wastewater and then adding the aniline degradation bacteria, the rapid COD degradation bacteria, the sludge having high aniline degradation activity and obtained in the step 1, the nutrients and the tourmaline to the aniline wastewater; 3, aerating and stirring the aniline wastewater in the bioreactor and replacing the aniline wastewater; 4, repeating step 3 and stopping replacing the aniline wastewater. The microorganisms and the method are applied to the field of sewage treatment.
Description
Technical field
The present invention relates to the microbial inoculum that a kind of aniline waste water starts fast and the method started fast.
Background technology
It is wide that aniline waste water carrys out source range, and pollution hazard is large, all discharges aniline waste water in the production of chemical industry, dyestuff production, medical supplies industry and application process.A large amount of production of aniline and widespread use, cause pollution to environment, also brings harm to human body.At present, the method for conventional process aniline waste water mainly contains solvent extration, absorption method, wet oxidation process, photocatalytic oxidation, supercritical oxidation, reverse osmosis method and biological process etc.Biochemical method aniline waste water has that energy consumption is low, secondary pollution is little, high treating effect.In order to improve the treatment effect of biochemical process to Aniline, the efficient degradation bacteria strains of Isolation and screening is very necessary.Want the bacterial strain stable existence in systems in which that Isolation and screening is gone out, and play better effect, biological reinforcing technology obtains the concern of domestic and international investigator.Morningstar lilies etc. adopt biological reinforced method to be added in sbr reactor system by the high efficient cryogenic microphenomenon of degradation of aniline of laboratory screening, (12 DEG C) achieve aniline waste water and effectively degrade under cryogenic, for the petrochemical wastewater containing aniline 174mg/L, the clearance of consolidation system to aniline reaches 97.8%.The microbial inoculum exploitation of current aniline waste water is less, and the treatment effect of sewage reality is bad simultaneously, does not find suitable technique.
Bacteria agent has the advantage of its uniqueness for process city domestic sewage and wastewater from chemical industry, can complete rapidly the startup of biochemical system in adverse conditions, and can significantly improve organic clearance, reduces the generation of solid matter, strengthening nitrification.But 3 primary conditions should be met for the microbial preparation of municipal wastewater treatment plant debugging: after 1) adding, microbial activity is high; 2) thalline can fast breeding in systems in which, shortens the time of microorganism culturing domestication, improves rapidly the concentration of microorganism in biological treatment system, thus increase work efficiency; 3) can not only existence be competed in systems in which, and can maintain a great deal of; 4) use safety, simple to operation, can play a role in real time, thus save the energy.
Summary of the invention
The present invention will solve current aniline waste water to start comparatively slow, the problem for the treatment of effect instability, the method for the microbial inoculum providing a kind of aniline waste water to start fast and fast startup aniline waste water.
The microbial inoculum that aniline waste water of the present invention starts fast comprises degrading aniline bacterial strain, fast COD degradation bacterial strain, active sludge, nutritive substance and tourmalinite.
Described degrading aniline bacterial strain comprises Chryseobacterium sp (Chryseobacteriumsp.) AN4, pseudomonas (Pseudomonas) 6
#and Delftiasp.GXA7.Described quick COD degradation bacterial strain is Aeromonas (Aeromonas) C3.
In the microbial inoculum that aniline waste water starts fast, the quantity of whole viable bacteria is greater than 10
6individual/mL, wherein Chryseobacterium sp AN4, pseudomonas 6
#, Delftiasp.GXA7 and Aeromonas C3 individually ferments, add with bacterium liquid form, the volume ratio of the bacterium liquid of 4 kinds of bacterium is 1:1:1:1.
Wherein Chryseobacterium sp AN4 is open in document " isolation identification of a strain microphenomenon of degradation of aniline and degradation characteristic thereof " (Zhang Yi flies, Environmental Pollution and Control, 2008.30 (2): 12-15); Pseudomonas 6
#open in document " screening of a strain microphenomenon of degradation of aniline and characteristic research thereof " (Miao Yanfang etc., Sichuan University's journal, in August, 2003, the 40th volume the 4th phase); Delftiasp.GXA7 is open in document " Screening and Identification of efficient degrading aniline bacterium GXA7 and aniline dioxygenase gene thereof bunch clone " (Bi Hongkai, Guangxi University's master thesis, 2004.6.1); Aeromonas C3 is open (Cai Sulan etc., environmental science and management, 2007.32 (10): 81-83) in document " Low Temperature-Resistant Strain C3 is to the experimental study of COD of sewage degradation property ".
Described active sludge is tamed by the mud of the second pond of municipal sewage plant, the high reactivity mud with degrading aniline of acquisition.The active sludge polluted adds in reactor, is cultivated, be conducive to the stability of guarantee system like this by the domestication carrying out aniline waste water.
Concrete acclimation method is as follows:
1. check aerobic reaction appliance arrangement, ensure oxygen reaction unit Plant in good condition;
2. the medicament of preparation domestication, the water containing 0.5 gram of urea, 2 grams of white sugars, 0.2 gram of ferrous sulfate, 0.1 gram of potassium primary phosphate, 0.1 gram of SODIUMNITRATE, 0.1 gram of magnesium sulfate, 2 milliliters of Sodium.alpha.-hydroxypropionates, 2 grams of sodium sulfate, 0.01 gram of calcium chloride and surpluses in 1 hydrargyrum oxydatum crudum agent;
3. the mud of the second pond of municipal sewage plant is stirred, remove the particulate matter that diameter is greater than 2mm, then add aniline waste water, make sludge concentration reach 3000mg/L;
4. then add step medicament 2., medicament is 1:20 with the volume ratio of the rear mud of dilution, stirs, pours in aerobic reaction device;
5. then carry out aeration, make the content of dissolved oxygen be 4mg/L ~ 6mg/L, aeration time is 72 hours;
6. monitor COD, supplement medicament during COD value <200mg/L, the volume ratio of medicament and aniline waste water is 1:20; Ensure sludge quantity, the height of quiet heavy rear mud is at more than 3/5 of container height simultaneously;
7. an aniline waste water is changed as COD value <100mg/L;
8. then in aerobic reaction unit, enter aniline waste water, HRT (hydraulic detention time) controls at 24 hours, namely continues to obtain the high reactivity mud with degrading aniline.
Described nutritive substance is made up of ferro element, nitrogen element and trace element, and the mass ratio of ferro element, nitrogen element and trace element is 3:6:1, and its medium trace element is by FeSO
40.3g, CuSO
45H
2o0.038g, ZnSO
47H
2o0.115g, MnSO
40.169g, H
3bO
30.116g, CoCl6H
2o0.024g and Na
2moO
42H
2o0.017g forms.The interpolation of nutritive substance makes the growth that system microorganism can be stable.
Adopt tourmalinite as growth stimulant, the particle size range of tourmalinite, below 2000 orders, is necessary for the tourmalinite of boracic simultaneously.The dosage of tourmalinite is: in pending waste water, the concentration of tourmalinite is 2mg/L.
The method that the microbial inoculum utilizing above-mentioned aniline waste water to start fast starts aniline waste water is fast realized by following steps:
One, mud is tamed, obtain the highly active mud with degrading aniline;
Two, in the bio-reactor of process aniline waste water, fill it up with aniline waste water, in aniline waste water, then add degrading aniline bacterial strain, fast COD degradation bacterial strain, the highly active mud with degrading aniline, nutritive substance and tourmalinite that step one obtains,
Three, aeration and stirring are carried out to the aniline waste water in bio-reactor, the dissolved oxygen concentration controlled in aniline waste water is 4mg/L ~ 6mg/L, and monitors, during COD value <100mg/L the COD of aniline waste water, stop aeration and stirring, change aniline waste water;
Four, the operation of repeating step three, until the COD value of aniline waste water reaches 100mg/L when HRT is 8h, stops changing aniline waste water, the i.e. quick start method of aniline waste water.
Beneficial effect of the present invention:
By the domestication of mud and the interpolation of microbial inoculum, carry out the biological reinforced of microbial inoculum, simultaneously by the adjustment of Sewage treatment systems, the interpolation of Inlet and outlet water flow and nutritive element and promotor, is conducive to the quick startup of the stable of technique and aniline waste water.The rapid boot-up time finally realizing aniline waste water technique is 7 days, can ensure the steady removal of technique simultaneously.
The present invention adopts SBR technique, carry out the quick startup of High Concentration Aniline Wastewater, biological reinforced system effectively can realize the degraded to aniline, after system reaches steady running, degrading aniline rate is 100%, the clearance of COD is about 93%, and effluent quality reaches national secondary discharge standard, can realize 7 days sewage and start fast.
The present invention has mainly carried out aniline waste water SBR technical study under cold condition, adopts efficient degrading aniline flora, high-efficiency activated mud and growth stimulant to carry out biological reinforced, constructs aniline and start microbial inoculum fast, be optimized simultaneously to technique.Industrial application for aniline waste water biological reinforcing technology provides most important theories basis.
Accompanying drawing explanation
Fig. 1 is the stereoscan photograph of the 2nd day active sludge in embodiment 1;
Fig. 2 is the stereoscan photograph of the 6th day active sludge in embodiment 1;
Fig. 3 is the stereoscan photograph of the 15th day active sludge in embodiment 1;
Fig. 4 is the changing conditions of aniline mass concentration in sbr reactor device in embodiment 1; In figure, ■ represents water inlet, ● represent water outlet, △ represents clearance;
Fig. 5 is the changing conditions of COD mass concentration in sbr reactor device in embodiment 1, and in figure, ■ represents water inlet, ● represent water outlet, △ represents clearance.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: the microbial inoculum that present embodiment aniline waste water starts fast comprises degrading aniline bacterial strain, fast COD degradation bacterial strain, active sludge, nutritive substance and tourmalinite.
Embodiment two: present embodiment and embodiment one unlike: described degrading aniline bacterial strain comprises Chryseobacterium sp AN4, pseudomonas 6
#and Delftiasp.GXA7.Described quick COD degradation bacterial strain is Aeromonas C3.Other is identical with embodiment one.
Embodiment three: present embodiment and embodiment one unlike: in the microbial inoculum that aniline waste water starts fast, the quantity of whole viable bacteria is greater than 10
6individual/mL, wherein Chryseobacterium sp AN4, pseudomonas 6
#, Delftiasp.GXA7 and Aeromonas C3 individually ferments, add with bacterium liquid form, the volume ratio of the bacterium liquid of 4 kinds of bacterium is 1:1:1:1.Other is identical with embodiment one.
Embodiment four: present embodiment and embodiment one unlike: described active sludge is tamed by the mud of the second pond of municipal sewage plant, the high reactivity mud with degrading aniline of acquisition, and concrete acclimation method is as follows:
1. check aerobic reaction appliance arrangement, ensure oxygen reaction unit Plant in good condition;
2. the medicament of preparation domestication, the water containing 0.5 gram of urea, 2 grams of white sugars, 0.2 gram of ferrous sulfate, 0.1 gram of potassium primary phosphate, 0.1 gram of SODIUMNITRATE, 0.1 gram of magnesium sulfate, 2 milliliters of Sodium.alpha.-hydroxypropionates, 2 grams of sodium sulfate, 0.01 gram of calcium chloride and surpluses in 1 hydrargyrum oxydatum crudum agent;
3. the mud of the second pond of municipal sewage plant is stirred, remove the particulate matter that diameter is greater than 2mm, then add aniline waste water, make sludge concentration reach 3000mg/L;
4. then add step medicament 2., medicament is 1:20 with the volume ratio of the rear mud of dilution, stirs, pours in aerobic reaction device;
5. then carry out aeration, make the content of dissolved oxygen be 4mg/L ~ 6mg/L, aeration time is 72 hours;
6. monitor COD, supplement medicament during COD value <200mg/L, the volume ratio of medicament and aniline waste water is 1:20; Ensure sludge quantity, the height of quiet heavy rear mud is at more than 3/5 of container height simultaneously;
7. an aniline waste water is changed as COD value <100mg/L;
8. then in aerobic reaction unit, enter aniline waste water, HRT controls at 24 hours, namely continues to obtain the high reactivity mud with degrading aniline.Other is identical with embodiment one.
Embodiment five: present embodiment and embodiment one unlike: described nutritive substance is made up of ferro element, nitrogen element and trace element, and the mass ratio of ferro element, nitrogen element and trace element is 3:6:1, and its medium trace element is by FeSO
40.3g, CuSO
45H
2o0.038g, ZnSO
47H
2o0.115g, MnSO
40.169g, H
3bO
30.116g, CoCl6H
2o0.024g and Na
2moO
42H
2o0.017g forms.Other is identical with embodiment one.
Embodiment six: present embodiment and embodiment one unlike: the particle diameter of described tourmalinite is below 2000 orders, is necessary for the tourmalinite of boracic simultaneously, and the concentration of the tourmalinite added in pending waste water is 2mg/L.Other is identical with embodiment one.
Embodiment seven: the method that the microbial inoculum that present embodiment utilizes aniline waste water to start fast starts aniline waste water is fast realized by following steps:
One, mud is tamed, obtain the highly active mud with degrading aniline;
Two, in the bio-reactor of process aniline waste water, fill it up with aniline waste water, in aniline waste water, then add degrading aniline bacterial strain, fast COD degradation bacterial strain, the highly active mud with degrading aniline, nutritive substance and tourmalinite that step one obtains,
Three, aeration and stirring are carried out to the aniline waste water in bio-reactor, the dissolved oxygen concentration controlled in aniline waste water is 4mg/L ~ 6mg/L, and monitors, during COD value <100mg/L the COD of aniline waste water, stop aeration and stirring, change aniline waste water;
Four, the operation of repeating step three, until the COD value of aniline waste water reaches 100mg/L when HRT is 8h, stops changing aniline waste water, the i.e. quick start method of aniline waste water.
Embodiment eight: present embodiment and embodiment seven unlike: in step one, acclimation method is as follows:
1. check aerobic reaction appliance arrangement, ensure oxygen reaction unit Plant in good condition;
2. the medicament of preparation domestication, the water containing 0.5 gram of urea, 2 grams of white sugars, 0.2 gram of ferrous sulfate, 0.1 gram of potassium primary phosphate, 0.1 gram of SODIUMNITRATE, 0.1 gram of magnesium sulfate, 2 milliliters of Sodium.alpha.-hydroxypropionates, 2 grams of sodium sulfate, 0.01 gram of calcium chloride and surpluses in 1 hydrargyrum oxydatum crudum agent;
3. the mud of the second pond of municipal sewage plant is stirred, remove the particulate matter that diameter is greater than 2mm, then add aniline waste water, make sludge concentration reach 3000mg/L;
4. then add step medicament 2., medicament is 1:20 with the volume ratio of the rear mud of dilution, stirs, pours in aerobic reaction device;
5. then carry out aeration, make the content of dissolved oxygen be 4mg/L ~ 6mg/L, aeration time is 72 hours;
6. monitor COD, supplement medicament during COD value <200mg/L, the volume ratio of medicament and aniline waste water is 1:20; Ensure sludge quantity, the height of quiet heavy rear mud is at more than 3/5 of container height simultaneously;
7. an aniline waste water is changed as COD value <100mg/L;
8. then in aerobic reaction unit, enter aniline waste water, HRT controls at 24 hours, namely continues to obtain the high reactivity mud with degrading aniline.Other is identical with embodiment seven.
Embodiment nine: present embodiment and embodiment seven unlike: the bacterial strain of degrading aniline described in step 2 comprises Chryseobacterium sp AN4, pseudomonas 6
#and Delftiasp.GXA7, described quick COD degradation bacterial strain is Aeromonas C3.Other is identical with embodiment seven.
Embodiment ten: present embodiment and embodiment seven unlike: add degrading aniline bacterial strain and quick COD degradation bacterial strain in step 2, make the quantity of whole viable bacteria in waste water be greater than 10
6individual/mL, wherein Chryseobacterium sp AN4, pseudomonas 6
#, Delftiasp.GXA7 and Aeromonas C3 individually ferments, add with bacterium liquid form, the volume ratio of the bacterium liquid of 4 kinds of bacterium is 1:1:1:1.Other is identical with embodiment seven.
Embodiment 11: present embodiment and embodiment seven unlike: step 2 Middle nutrition material is made up of ferro element, nitrogen element and trace element, and the mass ratio of ferro element, nitrogen element and trace element is 3:6:1, and its medium trace element is by FeSO
40.3g, CuSO
45H
2o0.038g, ZnSO
47H
2o0.115g, MnSO
40.169g, H
3bO
30.116g, CoCl6H
2o0.024g and Na
2moO
42H
2o0.017g forms.Other is identical with embodiment seven.
Embodiment 12: present embodiment and embodiment seven unlike: the particle diameter of described tourmalinite is below 2000 orders, is necessary for the tourmalinite of boracic simultaneously, and the concentration of the tourmalinite added in pending waste water is 2mg/L.Other is identical with embodiment seven.
For verifying beneficial effect of the present invention, carry out following test:
Embodiment 1:
The microbial inoculum that the present embodiment aniline waste water starts fast comprises degrading aniline bacterial strain, fast COD degradation bacterial strain, active sludge, nutritive substance and tourmalinite.
Described degrading aniline bacterial strain comprises Chryseobacterium sp (Chryseobacteriumsp.) AN4, pseudomonas (Pseudomonas) 6
#and Delftiasp.GXA7.Described quick COD degradation bacterial strain is Aeromonas (Aeromonas) C3.
In the microbial inoculum that aniline waste water starts fast, the quantity of whole viable bacteria is greater than 10
6individual/mL, wherein Chryseobacterium sp AN4, pseudomonas 6
#, Delftiasp.GXA7 and Aeromonas C3 individually ferments, add with bacterium liquid form, the volume ratio of the bacterium liquid of 4 kinds of bacterium is 1:1:1:1.
Wherein Chryseobacterium sp AN4 is open in document " isolation identification of a strain microphenomenon of degradation of aniline and degradation characteristic thereof " (Zhang Yi flies, Environmental Pollution and Control, 2008.30 (2): 12-15); Pseudomonas 6
#open in document " screening of a strain microphenomenon of degradation of aniline and characteristic research thereof " (Miao Yanfang etc., Sichuan University's journal, in August, 2003, the 40th volume the 4th phase); Delftiasp.GXA7 is open in document " Screening and Identification of efficient degrading aniline bacterium GXA7 and aniline dioxygenase gene thereof bunch clone " (Bi Hongkai, Guangxi University's master thesis, 2004.6.1); Aeromonas C3 is open (Cai Sulan etc., environmental science and management, 2007.32 (10): 81-83) in document " Low Temperature-Resistant Strain C3 is to the experimental study of COD of sewage degradation property ".
Described active sludge is tamed by the mud of the second pond of municipal sewage plant, the high reactivity mud with degrading aniline of acquisition.The active sludge polluted adds in reactor, is cultivated, be conducive to the stability of guarantee system like this by the domestication carrying out aniline waste water.
Concrete acclimation method is as follows:
1. check aerobic reaction appliance arrangement, ensure oxygen reaction unit Plant in good condition;
2. the medicament of preparation domestication, the water containing 0.5 gram of urea, 2 grams of white sugars, 0.2 gram of ferrous sulfate, 0.1 gram of potassium primary phosphate, 0.1 gram of SODIUMNITRATE, 0.1 gram of magnesium sulfate, 2 milliliters of Sodium.alpha.-hydroxypropionates, 2 grams of sodium sulfate, 0.01 gram of calcium chloride and surpluses in 1 hydrargyrum oxydatum crudum agent;
3. the mud of the second pond of municipal sewage plant is stirred, remove the particulate matter that diameter is greater than 2mm, then add aniline waste water, make sludge concentration reach 3000mg/L;
4. then add step medicament 2., medicament is 1:20 with the volume ratio of the rear mud of dilution, stirs, pours in aerobic reaction device;
5. then carry out aeration, make the content of dissolved oxygen be 4mg/L ~ 6mg/L, aeration time is 72 hours;
6. monitor COD, supplement medicament during COD value <200mg/L, the volume ratio of medicament and aniline waste water is 1:20; Ensure sludge quantity, the height of quiet heavy rear mud is at more than 3/5 of container height simultaneously;
7. an aniline waste water is changed as COD value <100mg/L;
8. then in aerobic reaction unit, enter aniline waste water, HRT (hydraulic detention time) controls at 24 hours, namely continues to obtain the high reactivity mud with degrading aniline.
Described nutritive substance is made up of ferro element, nitrogen element and trace element, and the mass ratio of ferro element, nitrogen element and trace element is 3:6:1, and its medium trace element is by FeSO
40.3g, CuSO
45H
2o0.038g, ZnSO
47H
2o0.115g, MnSO
40.169g, H
3bO
30.116g, CoCl6H
2o0.024g and Na
2moO
42H
2o0.017g forms.The interpolation of nutritive substance makes the growth that system microorganism can be stable.
Adopt tourmalinite as growth stimulant, the particle size range of tourmalinite, below 2000 orders, is necessary for the tourmalinite of boracic simultaneously.The dosage of tourmalinite is: in pending waste water, the concentration of tourmalinite is 2mg/L.
The method that the microbial inoculum utilizing above-mentioned aniline waste water to start fast starts fast is realized by following steps:
One, mud is tamed, obtain the highly active mud with degrading aniline;
Two, in the bio-reactor of process aniline waste water, fill it up with aniline waste water, in aniline waste water, then add degrading aniline bacterial strain, fast COD degradation bacterial strain, the highly active mud with degrading aniline, nutritive substance and tourmalinite that step one obtains; The addition of nutritive substance is: the volume of nutritive substance is 5% of aniline waste water volume;
Three, aeration and stirring are carried out to the aniline waste water in bio-reactor, the dissolved oxygen concentration controlled in aniline waste water is 4mg/L ~ 6mg/L, and monitors, during COD value <100mg/L the COD of aniline waste water, stop aeration and stirring, change aniline waste water;
Four, the operation of repeating step three, until the COD value of aniline waste water reaches 100mg/L when HRT is 8h, stops changing aniline waste water, the i.e. quick start method of aniline waste water.The rapid boot-up time finally realizing aniline waste water technique is 7 days.
Carry out the process of aniline waste water afterwards.
Fig. 1 is the stereoscan photograph of process the 2nd day active sludge, and Fig. 2 is the stereoscan photograph of process the 6th day active sludge, and Fig. 3 is the stereoscan photograph of process the 15th day active sludge, and magnification is 5000 times.As can be seen from Fig. 1-3, the content of microorganisms of the 6th day active sludge will more than the content of microorganisms of the 2nd day active sludge.Microorganism on 15th day active sludge is more, film forming.
In sbr reactor device, the changing conditions of aniline mass concentration as shown in Figure 4, and after after 72h, the clearance of aniline reaches more than 80%, 120h as shown in Figure 4, the clearance of aniline is 100%, and aniline effluent characteristics concentration is almost nil.Illustrate that the inventive method can realize the effective removal to aniline.
In sbr reactor device, the changing conditions of COD mass concentration as shown in Figure 5, and after system stable operation, in water outlet, COD mass concentration remains on about 100mg/L as shown in Figure 5, and clearance is 92.23%.Illustrate that the inventive method can realize the effective removal to COD.
Microphenomenon of degradation of aniline can be good at being fixed on active sludge and is formed through biological reinforced active sludge, and when system reaches stable, microphenomenon of degradation of aniline is dispersed throughout on active sludge.Microbial Community Diversity enriches, and dominant population is comparatively obvious.Through the operation in one period, structure of community creates obvious otherness, and in system, microorganism dominant strain is strengthened, and water outlet reaches secondary standard.
Claims (9)
1. the microbial inoculum that starts fast of aniline waste water, is characterized in that this microbial inoculum comprises degrading aniline bacterial strain, fast COD degradation bacterial strain, active sludge, nutritive substance and tourmalinite.
2. the microbial inoculum that starts fast of a kind of aniline waste water according to claim 1, is characterized in that described degrading aniline bacterial strain comprises Chryseobacterium sp AN4, pseudomonas 6
#and Delftiasp.GXA7, described quick COD degradation bacterial strain is Aeromonas C3.
3. the microbial inoculum that starts fast of a kind of aniline waste water according to claim 1, is characterized in that the quantity of whole viable bacteria in the microbial inoculum that aniline waste water starts fast is greater than 10
6individual/mL, wherein Chryseobacterium sp AN4, pseudomonas 6
#, Delftiasp.GXA7 and Aeromonas C3 individually ferments, add with bacterium liquid form, the volume ratio of the bacterium liquid of 4 kinds of bacterium is 1:1:1:1.
4. the microbial inoculum that starts fast of a kind of aniline waste water according to claim 1, it is characterized in that described active sludge is tamed by the mud of the second pond of municipal sewage plant, the high reactivity mud with degrading aniline obtained, concrete acclimation method is as follows:
1. check aerobic reaction appliance arrangement, ensure oxygen reaction unit Plant in good condition;
2. the medicament of preparation domestication, the water containing 0.5 gram of urea, 2 grams of white sugars, 0.2 gram of ferrous sulfate, 0.1 gram of potassium primary phosphate, 0.1 gram of SODIUMNITRATE, 0.1 gram of magnesium sulfate, 2 milliliters of Sodium.alpha.-hydroxypropionates, 2 grams of sodium sulfate, 0.01 gram of calcium chloride and surpluses in 1 hydrargyrum oxydatum crudum agent;
3. the mud of the second pond of municipal sewage plant is stirred, remove the particulate matter that diameter is greater than 2mm, then add aniline waste water, make sludge concentration reach 3000mg/L;
4. then add step medicament 2., medicament is 1:20 with the volume ratio of the rear mud of dilution, stirs, pours in aerobic reaction device;
5. then carry out aeration, make the content of dissolved oxygen be 4mg/L ~ 6mg/L, aeration time is 72 hours;
6. monitor COD, supplement medicament during COD value <200mg/L, the volume ratio of medicament and aniline waste water is 1:20; Ensure sludge quantity, the height of quiet heavy rear mud is at more than 3/5 of container height simultaneously;
7. an aniline waste water is changed as COD value <100mg/L;
8. then in aerobic reaction unit, enter aniline waste water, HRT controls at 24 hours, namely continues to obtain the high reactivity mud with degrading aniline.
5. the microbial inoculum that starts fast of a kind of aniline waste water according to claim 1, it is characterized in that described nutritive substance is made up of ferro element, nitrogen element and trace element, the mass ratio of ferro element, nitrogen element and trace element is 3:6:1, and its medium trace element is by FeSO
40.3g, CuSO
45H
2o0.038g, ZnSO
47H
2o0.115g, MnSO
40.169g, H
3bO
30.116g, CoCl6H
2o0.024g and Na
2moO
42H
2o0.017g forms.
6. the microbial inoculum that starts fast of a kind of aniline waste water according to claim 1, it is characterized in that the particle diameter of described tourmalinite is below 2000 orders, be necessary for the tourmalinite of boracic simultaneously, the concentration of the tourmalinite added in pending waste water is 2mg/L.
7. the microbial inoculum utilizing the aniline waste water described in claim 1 to start fast starts the method for aniline waste water fast, it is characterized in that the method is realized by following steps:
One, mud is tamed, obtain the highly active mud with degrading aniline;
Two, in the bio-reactor of process aniline waste water, fill it up with aniline waste water, in aniline waste water, then add degrading aniline bacterial strain, fast COD degradation bacterial strain, the highly active mud with degrading aniline, nutritive substance and tourmalinite that step one obtains,
Three, aeration and stirring are carried out to the aniline waste water in bio-reactor, the dissolved oxygen concentration controlled in aniline waste water is 4mg/L ~ 6mg/L, and monitors, during COD value <100mg/L the COD of aniline waste water, stop aeration and stirring, change aniline waste water;
Four, the operation of repeating step three, until the COD value of aniline waste water reaches 100mg/L when HRT is 8h, stops changing aniline waste water, the i.e. quick start method of aniline waste water.
8. the method for quick startup aniline waste water according to claim 7, is characterized in that in step one, acclimation method is as follows:
1. check aerobic reaction appliance arrangement, ensure oxygen reaction unit Plant in good condition;
2. the medicament of preparation domestication, the water containing 0.5 gram of urea, 2 grams of white sugars, 0.2 gram of ferrous sulfate, 0.1 gram of potassium primary phosphate, 0.1 gram of SODIUMNITRATE, 0.1 gram of magnesium sulfate, 2 milliliters of Sodium.alpha.-hydroxypropionates, 2 grams of sodium sulfate, 0.01 gram of calcium chloride and surpluses in 1 hydrargyrum oxydatum crudum agent;
3. the mud of the second pond of municipal sewage plant is stirred, remove the particulate matter that diameter is greater than 2mm, then add aniline waste water, make sludge concentration reach 3000mg/L;
4. then add step medicament 2., medicament is 1:20 with the volume ratio of the rear mud of dilution, stirs, pours in aerobic reaction device;
5. then carry out aeration, make the content of dissolved oxygen be 4mg/L ~ 6mg/L, aeration time is 72 hours;
6. monitor COD, supplement medicament during COD value <200mg/L, the volume ratio of medicament and aniline waste water is 1:20; Ensure sludge quantity, the height of quiet heavy rear mud is at more than 3/5 of container height simultaneously;
7. an aniline waste water is changed as COD value <100mg/L;
8. then in aerobic reaction unit, enter aniline waste water, HRT controls at 24 hours, namely continues to obtain the high reactivity mud with degrading aniline.
9. the method for quick startup aniline waste water according to claim 7, is characterized in that the dosage of tourmalinite in step 2 is: in pending waste water, the concentration of tourmalinite is 2mg/L.
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| CN106635918A (en) * | 2017-01-16 | 2017-05-10 | 轻工业环境保护研究所 | Delftiasp and application thereof to reduction and dehydration of excess sludge |
| CN109534482A (en) * | 2018-12-24 | 2019-03-29 | 哈尔滨工业大学 | The method for treating water of aniline pollution in a kind of emergency processing drinking water |
| CN114455696A (en) * | 2022-02-23 | 2022-05-10 | 哈尔滨工业大学（深圳） | SBR reactor activated sludge domestication enhancing method based on tourmaline |
| CN115180716A (en) * | 2022-07-12 | 2022-10-14 | 国环电池科技(苏州)有限公司 | Arsenic-containing wastewater treatment method based on sulfur cycle |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106635918A (en) * | 2017-01-16 | 2017-05-10 | 轻工业环境保护研究所 | Delftiasp and application thereof to reduction and dehydration of excess sludge |
| CN106635918B (en) * | 2017-01-16 | 2020-04-17 | 轻工业环境保护研究所 | Delftia sp and application thereof in excess sludge reduction and dehydration |
| CN109534482A (en) * | 2018-12-24 | 2019-03-29 | 哈尔滨工业大学 | The method for treating water of aniline pollution in a kind of emergency processing drinking water |
| CN114455696A (en) * | 2022-02-23 | 2022-05-10 | 哈尔滨工业大学（深圳） | SBR reactor activated sludge domestication enhancing method based on tourmaline |
| CN115180716A (en) * | 2022-07-12 | 2022-10-14 | 国环电池科技(苏州)有限公司 | Arsenic-containing wastewater treatment method based on sulfur cycle |
| CN115180716B (en) * | 2022-07-12 | 2024-02-23 | 华辰环保能源(广州)有限责任公司 | Sulfur circulation-based arsenic-containing wastewater treatment method |
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