CN103189511B - 利用siRNA导入的新型hiPSC制作法 - Google Patents
利用siRNA导入的新型hiPSC制作法 Download PDFInfo
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- CN103189511B CN103189511B CN201180043814.8A CN201180043814A CN103189511B CN 103189511 B CN103189511 B CN 103189511B CN 201180043814 A CN201180043814 A CN 201180043814A CN 103189511 B CN103189511 B CN 103189511B
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/713—Double-stranded nucleic acids or oligonucleotides
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- A61P1/16—Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
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- A61P21/00—Drugs for disorders of the muscular or neuromuscular system
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2320/00—Applications; Uses
- C12N2320/30—Special therapeutic applications
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/65—MicroRNA
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Epidemiology (AREA)
- Cell Biology (AREA)
- Transplantation (AREA)
- Developmental Biology & Embryology (AREA)
- Neurology (AREA)
- Gastroenterology & Hepatology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Physical Education & Sports Medicine (AREA)
- Neurosurgery (AREA)
- Pulmonology (AREA)
- Dermatology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2010-158192 | 2010-07-12 | ||
| JP2010-158193 | 2010-07-12 | ||
| JP2010158194 | 2010-07-12 | ||
| JP2010158192 | 2010-07-12 | ||
| JP2010-158194 | 2010-07-12 | ||
| JP2010158193 | 2010-07-12 | ||
| PCT/JP2011/064846 WO2012008301A1 (ja) | 2010-07-12 | 2011-06-28 | siRNA導入による新規hiPSC作製法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103189511A CN103189511A (zh) | 2013-07-03 |
| CN103189511B true CN103189511B (zh) | 2016-10-12 |
Family
ID=45469308
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180043814.8A Expired - Fee Related CN103189511B (zh) | 2010-07-12 | 2011-06-28 | 利用siRNA导入的新型hiPSC制作法 |
| CN201180043810XA Pending CN103097535A (zh) | 2010-07-12 | 2011-06-28 | 利用miRNA导入的新型hiPSC制作法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180043810XA Pending CN103097535A (zh) | 2010-07-12 | 2011-06-28 | 利用miRNA导入的新型hiPSC制作法 |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US9476041B2 (enExample) |
| EP (2) | EP2594644B1 (enExample) |
| JP (5) | JP5099571B2 (enExample) |
| CN (2) | CN103189511B (enExample) |
| WO (2) | WO2012008302A1 (enExample) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012008302A1 (ja) | 2010-07-12 | 2012-01-19 | 国立大学法人鳥取大学 | miRNA導入による新規hiPSC作製法 |
| EP4086338A1 (en) | 2011-03-17 | 2022-11-09 | Minerva Biotechnologies Corporation | Method for making pluripotent stem cells |
| WO2013059373A2 (en) | 2011-10-17 | 2013-04-25 | Minerva Biotechnologies Corporation | Media for stem cell proliferation and induction |
| US10034902B2 (en) * | 2012-02-22 | 2018-07-31 | Exostem Biotec Ltd. | MicroRNAs for the generation of astrocytes |
| JP6748430B2 (ja) * | 2012-07-13 | 2020-09-02 | ミネルバ バイオテクノロジーズ コーポレーション | より未分化状態への細胞の誘導方法 |
| US10265347B2 (en) | 2013-08-29 | 2019-04-23 | Norimasa Miura | Biomolecular group related to cell anti-aging |
| US20160289678A1 (en) * | 2013-11-22 | 2016-10-06 | Dcb-Usa Llc | Use of microrna 146-a in the diagnosis, treatment and prevention of picornavirus infection and microrna 146-a antagonists |
| CN104109670B (zh) * | 2014-03-12 | 2018-01-05 | 首都医科大学附属北京安定医院 | 一种双链siRNA分子及其应用 |
| SG11201610745XA (en) | 2014-06-27 | 2017-01-27 | Rhizen Pharmaceuticals Sa | Substituted chromene derivatives as selective dual inhibitors of pi3 delta and gamma protein kinases |
| EP3286335A4 (en) | 2015-04-24 | 2018-10-17 | The Johns Hopkins University | Compositions and methods related to characterizing proviral reservoirs |
| WO2017073689A1 (ja) * | 2015-10-30 | 2017-05-04 | 国立大学法人鳥取大学 | Dna損傷を修復するrna分子 |
| KR102011336B1 (ko) * | 2015-12-31 | 2019-08-16 | 인터올리고 주식회사 | 약물 전달 및 안정화를 위한 복합체 및 그 제조방법 |
| WO2018203553A1 (ja) * | 2017-05-02 | 2018-11-08 | 株式会社ペジィー・ファーマ | 分化細胞の品質改善方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006112239A1 (ja) * | 2005-04-15 | 2006-10-26 | National University Corporation Tottori University | hTERT発現調節遺伝子 |
| CN101333524A (zh) * | 2008-06-25 | 2008-12-31 | 中山大学 | 一个小分子非编码RNA基因hsa-miR-101及其抗肿瘤用途 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1452594A4 (en) * | 2001-11-15 | 2007-01-10 | Kyowa Hakko Kogyo Kk | INDUCTOR FOR DIFFERENTIATING EMBRYOONAL STEM CELLS TO EODODERMAL CELLS, METHOD FOR OBTAINING THEM AND USE THEREOF |
| WO2004024940A2 (en) | 2002-09-16 | 2004-03-25 | University Of Southern California | Rna-mediated gene modulation |
| KR20050115231A (ko) | 2003-02-10 | 2005-12-07 | 내셔날 인스티튜트 오브 어드밴스드 인더스트리얼 사이언스 앤드 테크놀로지 | 포유동물 세포의 조절 |
| US9453219B2 (en) | 2003-05-15 | 2016-09-27 | Mello Biotech Taiwan Co., Ltd. | Cosmetic designs and products using intronic RNA |
| EP2314688B1 (en) | 2004-11-12 | 2014-07-16 | Asuragen, Inc. | Methods and compositions involving miRNA and miRNA inhibitor molecules |
| WO2006084275A2 (en) | 2005-02-04 | 2006-08-10 | Auburn University | Contact drug delivery system |
| PL2002003T3 (pl) * | 2005-05-27 | 2016-06-30 | Ospedale San Raffaele Srl | Wektor genetyczny zawierający mi-RNA |
| WO2006137514A1 (ja) | 2005-06-23 | 2006-12-28 | Locomogene, Inc. | シノビオリンの発現もしくは機能阻害物質を有効成分とする癌治療剤、および癌治療剤のスクリーニング方法 |
| KR101420740B1 (ko) | 2005-12-13 | 2014-07-17 | 교또 다이가꾸 | 핵초기화 인자 |
| EP2487260B1 (en) | 2006-01-05 | 2015-07-08 | The Ohio State University Research Foundation | Microrna-based methods and compositions for the diagnosis and treatment of solid cancers |
| KR101407707B1 (ko) | 2006-04-03 | 2014-06-19 | 산타리스 팔마 에이/에스 | Anti-mirna 안티센스 올리고뉴클레오타이드를 함유하는 약학적 조성물 |
| CN101980712B (zh) * | 2007-10-29 | 2015-02-18 | 雷古拉斯治疗公司 | 用于肝癌治疗的靶向微小rna |
| KR101564044B1 (ko) | 2007-10-31 | 2015-10-28 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 핵초기화 방법 |
| KR101532442B1 (ko) | 2007-12-10 | 2015-06-29 | 고쿠리츠 다이가쿠 호진 교토 다이가쿠 | 효율적인 핵 초기화 방법 |
| CA2710713C (en) * | 2007-12-27 | 2017-09-19 | Protiva Biotherapeutics, Inc. | Silencing of polo-like kinase expression using interfering rna |
| CN101970664B (zh) * | 2008-01-16 | 2013-08-21 | 林希龙 | 使用可诱导的重组核糖核酸因子生成不含肿瘤的类胚胎干细胞的多能性细胞 |
| CN101392251B (zh) * | 2008-11-03 | 2015-07-22 | 清华大学深圳研究生院 | 能诱导干细胞向成骨细胞分化的微小rna及其应用 |
| JP2010120881A (ja) * | 2008-11-19 | 2010-06-03 | Keio Gijuku | ヒト癌タンパク質MDM2とヒト癌抑制タンパク質p53との相互作用阻害ペプチド及びその使用 |
| WO2012008302A1 (ja) | 2010-07-12 | 2012-01-19 | 国立大学法人鳥取大学 | miRNA導入による新規hiPSC作製法 |
-
2011
- 2011-06-28 WO PCT/JP2011/064847 patent/WO2012008302A1/ja not_active Ceased
- 2011-06-28 WO PCT/JP2011/064846 patent/WO2012008301A1/ja not_active Ceased
- 2011-06-28 JP JP2011530189A patent/JP5099571B2/ja not_active Expired - Fee Related
- 2011-06-28 US US13/809,880 patent/US9476041B2/en not_active Expired - Fee Related
- 2011-06-28 US US13/809,882 patent/US9790491B2/en not_active Expired - Fee Related
- 2011-06-28 CN CN201180043814.8A patent/CN103189511B/zh not_active Expired - Fee Related
- 2011-06-28 JP JP2011530188A patent/JP5099570B2/ja not_active Expired - Fee Related
- 2011-06-28 CN CN201180043810XA patent/CN103097535A/zh active Pending
- 2011-06-28 EP EP11806632.3A patent/EP2594644B1/en not_active Not-in-force
- 2011-06-28 EP EP11806631.5A patent/EP2594643B1/en not_active Not-in-force
-
2012
- 2012-09-14 JP JP2012202509A patent/JP2013034479A/ja active Pending
- 2012-09-14 JP JP2012202508A patent/JP2013046616A/ja active Pending
-
2013
- 2013-12-20 JP JP2013264240A patent/JP5845493B2/ja not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006112239A1 (ja) * | 2005-04-15 | 2006-10-26 | National University Corporation Tottori University | hTERT発現調節遺伝子 |
| CN101333524A (zh) * | 2008-06-25 | 2008-12-31 | 中山大学 | 一个小分子非编码RNA基因hsa-miR-101及其抗肿瘤用途 |
Non-Patent Citations (1)
| Title |
|---|
| A noncoding RNA gene on chromosome 10p15.3 may function upstream of hTERT;Norimasa Miura等;《BMC Molecular Biology》;20090202;第10卷(第5期);第5页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103189511A (zh) | 2013-07-03 |
| CN103097535A (zh) | 2013-05-08 |
| JP5099571B2 (ja) | 2012-12-19 |
| JP2014097059A (ja) | 2014-05-29 |
| JP5099570B2 (ja) | 2012-12-19 |
| JPWO2012008301A1 (ja) | 2013-09-09 |
| JP2013046616A (ja) | 2013-03-07 |
| EP2594643B1 (en) | 2017-04-12 |
| EP2594644A1 (en) | 2013-05-22 |
| US9476041B2 (en) | 2016-10-25 |
| US9790491B2 (en) | 2017-10-17 |
| WO2012008302A1 (ja) | 2012-01-19 |
| JP2013034479A (ja) | 2013-02-21 |
| EP2594644A4 (en) | 2014-12-24 |
| EP2594644B1 (en) | 2016-08-10 |
| EP2594643A1 (en) | 2013-05-22 |
| EP2594643A4 (en) | 2014-12-24 |
| US20130190389A1 (en) | 2013-07-25 |
| JPWO2012008302A1 (ja) | 2013-09-09 |
| US20130184335A1 (en) | 2013-07-18 |
| JP5845493B2 (ja) | 2016-01-20 |
| WO2012008301A1 (ja) | 2012-01-19 |
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Granted publication date: 20161012 Termination date: 20180628 |