CN103185789A - RV-ADV antigen feces rapid detector - Google Patents

RV-ADV antigen feces rapid detector Download PDF

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Publication number
CN103185789A
CN103185789A CN2011104448297A CN201110444829A CN103185789A CN 103185789 A CN103185789 A CN 103185789A CN 2011104448297 A CN2011104448297 A CN 2011104448297A CN 201110444829 A CN201110444829 A CN 201110444829A CN 103185789 A CN103185789 A CN 103185789A
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China
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adv
antigen
collecting device
sample collecting
adv antigen
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CN2011104448297A
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Chinese (zh)
Inventor
闫荀
马鹏
贺南航
刘津
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SHENZHEN AISUER BIOLOGICAL TECHNOLOGY CO LTD
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SHENZHEN AISUER BIOLOGICAL TECHNOLOGY CO LTD
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Priority to CN2011104448297A priority Critical patent/CN103185789A/en
Publication of CN103185789A publication Critical patent/CN103185789A/en
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Abstract

The invention provides a RV-ADV antigen feces rapid detector, including: a plastic protective shell, a RV-ADV antigen immunochromatographic reagent strip placed in the plastic protective shell, and a sample collector. The RV-ADV antigen immunochromatographic reagent strip includes sequentially arranged a sample feeding zone, a reaction zone and a water suction zone. The sample collector includes a capillarity microporous matrix structure, including a handle portion, a plate portion and a combination portion for connecting the handle portion and the plate portion. The sample collector is used for collecting crissum residual feces samples of children with diarrhea, and separates and transports RV antigen, ADV antigen or RV and ADV antigen in the feces samples. The plastic protective shell includes a bottom plate and a face plate which is arranged to coordinate with the bottom plate. The face plate is provided with an observation window used for interpreting detection results. The observation window is arranged corresponding to the reaction zone of the RV-ADV antigen immunochromatographic reagent strip. The handle portion of the sampling collector is partially placed in an overlapped manner on the upper surface of the sample feeding zone of the RV-ADV antigen immunochromatographic reagent strip. The plate portion is stretched out of the plastic protective shell.

Description

RV-ADV antigen ight soil fast detecting instrument
Technical field
The present invention relates in particular to a kind of RV-ADV antigen ight soil fast detecting instrument that can effectively detect RV antigen in the fecal sample, ADV antigen or RV and ADV antigen about a kind of fast detecting instrument.
Background technology
Clinically, usually adopt access toilet to collect diarrhoea infant liquid state or semi liquid state fecal sample, utilize RV or/and ADV immunochromatography reagent bar or RV or/and ADV ELISA reagent detects in the fecal sample whether contain rotavirus and/or adenovirus (being that RV is or/and ADV antigen), are used for the cause of disease of diagnosis infant diarrhoea.This method often needs professional and technical personnel, professional testing equipment, and under specialized laboratory's condition, implement and finish detection, and adopt this method to collect and when transporting fecal sample, can cause fecal sample to spill and pollute relative article, close on personnel and environment etc., form the new infection sources and enlarge eqpidemic disease and propagate.
Summary of the invention
The object of the present invention is to provide a kind of RV-ADV antigen ight soil fast detecting instrument, effectively RV antigen, ADV antigen or RV and ADV antigen in the qualitative detection diarrhoea residual liquid state of infant crissum or the semi liquid state fecal sample, be used for diagnosis diarrhoea infant and whether infect rotavirus or adenovirus, this detector is not only applicable to medical institutions the diarrhoea infant is carried out rotavirus or adenovirus infection examination, to diagnose its cause of disease, be applicable to that also individuality or family detect voluntarily.
For achieving the above object, the invention provides a kind of RV-ADV antigen ight soil fast detecting instrument, comprise: the plastics containment vessel, place RV-ADV antigen immune chromatography reagent strip in the plastics containment vessel, and sample collecting device, this RV-ADV antigen immune chromatography reagent strip comprises sample application zone, reaction zone and the suction zones that sets gradually; Sample collecting device has capillary micropore matrix structure, the joint portion that comprises shank, board and connection shank and board, this sample collecting device be used for to be gathered diarrhoea infant crissum presence of residual feces sample, and separates voluntarily and carry RV-ADVRV antigen, ADV antigen or RV and ADV antigen in the fecal sample; The panel that the plastics containment vessel comprises base plate and is equipped with base plate, panel is provided with the watch window for the interpretation testing result, the corresponding setting of reaction zone of watch window and RV-ADV antigen immune chromatography reagent strip; The sample application zone top that the shank of sample collecting device is overlapped and is positioned over RV-ADV antigen immune chromatography reagent strip, board stretches out outside the plastics containment vessel; Described RV-ADV antigen immune chromatography reagent strip comprises RV antigen immune chromatography reagent strip, ADV antigen immune chromatography reagent strip, reaches the antigen combined immunochromatography reagent of RV+ADV bar.
Also comprise developping agent, be used with this RV-ADV antigen ight soil fast detecting instrument, make an addition on the sample collecting device, with the pH value of regulating fecal sample and directivity and the flow velocity that guarantees the fecal sample transportation, make RV-ADV antigen immune chromatography reagent strip obtain enough fecal samples, and promote RV antigen, ADV antigen or RV in the fecal sample to separate voluntarily with ADV antigen and carry; This developping agent splendid attire is in the transparent rigid plastic bottle of sealing.
Described developping agent is that a kind of pH value is the compound damping fluid of 6.5-8.5, and is formulated in proportion by potassium dihydrogen phosphate, trishydroxymethylaminomethane, sodium hydrogen phosphate, sodium chloride, potassium chloride, Tween-20, thimerosal, lauryl sodium sulfate, ethylenediamine tetraacetic acid, deionized water.
Described plastics containment vessel one end is made as the holding area, and the base plate of plastics containment vessel arranges several air holes in an end of holding area, and its inboard is made as the sample collecting device assembly section away from an end of holding area, and the sample collecting device stationary installation is set on it; The middle part is made as RV-ADV antigen immune chromatography reagent strip assembly section, and it is provided with for the stationary installation of placing RV-ADV antigen immune chromatography reagent strip.
Described watch window top is bonded with an eyeglass, is marked with " T ", " C " or " Tr ", " Ta " on the eyeglass, " C " indicates line, respectively with detection line and the corresponding setting of nature controlling line of RV-ADV antigen immune chromatography reagent strip; Eyeglass is made by transparent rigid plastic.
The base plate of described plastics containment vessel and panel inboard arrange coupling arrangement, are used for base plate is fixedlyed connected with panel, and fixed connection method adopts the method for withholding or adopts the ultrasonic plastic welder welding fixedly connected.
Described RV-ADV antigen immune chromatography reagent strip adopts colloid gold label, electroselenium mark, color latex particle mark or the preparation of fluorescent-substance markers technology.
Described sample collecting device is made by hydrophilic fibre and hydrophobic material, and this hydrophilic fibre and hydrophobic material form capillary micropore matrix structure; It is 8%~80% that hydrophilic fibre accounts for sample collecting device general assembly (TW) ratio range, and hydrophilic fibre is cotton fiber, filter paper, sponge or cloth; Hydrophobic material is tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, nylon and polyethersulfone and other plastics, glass or resin; The weight ratio scope of hydrophilic fibre and hydrophobic material is 1: 9 to 4: 1; The profile of sample collecting device is table tennis plate or slurry shape, and its total surface area is not less than 700 square millimeters, and cumulative volume is not less than 650 cubic millimeters, and the surface area of sample collecting device board is not less than sample collecting device total surface area 50%; 20~500 microns of the average pore sizes of capillary micropore matrix, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of sample collecting device is 0.21~0.86 gram/cubic centimetre.
Described sample collecting device is made by one or more hydrophobic materials, and this hydrophobic material forms capillary micropore matrix structure; Hydrophobic material is tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, polyester, nylon, polyethersulfone or other plastics, glass or resin; The sample collecting device profile is bar-shaped or tabular, and its total surface area is not less than 700 square millimeters, and cumulative volume is not less than 650 cubic millimeters, and the surface area of sample collecting device board is not less than 50% of sample collecting device total surface area; 20~500 microns of the average pore sizes of capillary micropore matrix structure, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of sample collecting device is 0.49~0.92 gram/cubic centimetre.
Beneficial effect of the present invention: the present invention is RV antigen, ADV antigen or RV and ADV antigen in qualitative detection diarrhoea infant crissum liquid state or the semi liquid state presence of residual feces sample effectively, be used for diagnosis diarrhoea infant and whether infect rotavirus or adenovirus, the propagation of RV or ADV is reduced to minimum degree, use access toilet and fecal sample transit link by evading, avoid related personnel, surrounding environment or article are polluted, reduce the new infection sources and occur; In addition, the present invention uses simply, and is easy to operate, is not only applicable to medical institutions the diarrhoea infant is carried out rotavirus or adenovirus infection examination, to diagnose its cause of disease, is applicable to that also individuality or family detect voluntarily, can be used as the standing health detection product of family.
Description of drawings
Below in conjunction with accompanying drawing, by the specific embodiment of the present invention is described in detail, will make technical scheme of the present invention and other beneficial effects apparent.
In the accompanying drawing,
Fig. 1 is the combination synoptic diagram of RV-ADV antigen ight soil fast detecting instrument of the present invention;
Fig. 2 is the decomposing schematic representation of Fig. 1;
Fig. 3 is the synoptic diagram of the RV-ADV antigen immune chromatography reagent strip among Fig. 2;
Fig. 4 is the synoptic diagram of the sample collecting device among Fig. 2;
Fig. 5 is the assembling synoptic diagram of each several part.
Embodiment
Shown in Fig. 1-5, RV-ADV antigen ight soil fast detecting instrument of the present invention comprises: plastics containment vessel 2, place RV-ADV antigen immune chromatography reagent strip 4 in the plastics containment vessel 2, and sample collecting device 6.
Wherein, RV-ADV antigen immune chromatography reagent strip 4 comprises sample application zone 42, reaction zone 44 and the suction zones 46 that sets gradually, and reaction zone 44 is connected between sample application zone 42 and the suction zones 46, and reaction zone 44 is provided with one or two detection lines 45 and nature controlling line 47; Plastics containment vessel 2 comprises base plate 22; the panel 24 that is equipped with base plate 22; base plate 22 is fixedlyed connected by its inboard coupling arrangement 29 that arranges with panel 24, and fixing means can adopt the method for withholding, and also can adopt the ultrasonic plastic welder welding fixedly connected.Plastics containment vessel 2 one ends are made as holding area 23, base plate 22 arranges several air holes 21 in holding area 23, base plate 22 inboards are made as sample collecting device assembly section 25 away from an end of holding area 23, and sample collecting device stationary installation 252 is set on it, are used for settling sample collecting device 6; The middle part is made as RV-ADV antigen immune chromatography reagent strip 4 assembly sections 26, the stationary installation 262 of RV-ADV antigen immune chromatography reagent strip 4 is set, in order to place RV-ADV antigen immune chromatography reagent strip 4 on it respectively.Panel 24 is provided with the watch window 27 for the interpretation testing result, watch window 27 and reaction zone 25 corresponding settings; Above watch window 27, be bonded with an eyeglass 28, be marked with " T ", " C " or " Tr ", " Ta " on the eyeglass 28, " C " indicates line, respectively with detection line 45 and the nature controlling line 47 corresponding settings of RV-ADV antigen immune chromatography reagent strip 4; Described eyeglass 28 is made by transparent rigid plastic.
Described RV-ADV antigen immune chromatography reagent strip 4 adopts colloid gold label, electroselenium mark, color latex particle mark or the preparation of fluorescent-substance markers technology, so is not limited thereto.This RV-ADV antigen immune chromatography reagent strip 4 comprises RV antigen immune chromatography reagent strip, ADV antigen immune chromatography reagent strip, reaches the antigen combined immunochromatography reagent of RV+ADV bar.
Described sample collecting device 6 has capillary micropore matrix structure, the joint portion 66 that comprises shank 62, board 64 and connection shank 62 and board 64, it be used for to gather the diarrhoea residual liquid state of infant crissum or semi liquid state fecal sample, and separates voluntarily and carry RV antigen, ADV antigen or RV and ADV in the fecal sample; The shank 62 of sample collecting device 6 is overlapped and is positioned over sample application zone 42 tops of RV-ADV antigen immune chromatography reagent strip 4, and board 64 stretches out outside the plastics containment vessel 2.Fecal sample and wherein RV antigen, ADV antigen or RV and ADV antigen are absorbed, separate, are delivered to the sample application zone 42 of RV-ADV antigen immune chromatography reagent strip 4 by sample collecting device 6, utilize chromatographic theory to be delivered to reaction zone 44 again, and a series of immunological responses take place at reaction zone 44, demonstrate the directly result of interpretation (as red stripes) of the watch window 27 of naked eyes by panel.
Described sample collecting device 6 is made by hydrophilic fibre and hydrophobic material, and this hydrophilic fibre and hydrophobic material form capillary micropore matrix structure, have the effect that separates voluntarily and carry the target detection thing in the fecal sample.It is 8%~80% that hydrophilic fibre accounts for sample collecting device general assembly (TW) ratio range, and hydrophilic fibre can be cotton fiber, filter paper, sponge, cloth; Hydrophobic material can be tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, polyester, nylon, polyethersulfone or other plastics, also can be glass, resin; The weight ratio scope of hydrophilic fibre and hydrophobic material is 1: 9 to 4: 1.These sample collecting device 6 profiles can be soldier's pang plate or oar shape, its parameter: total surface area is not less than 700 square millimeters, cumulative volume and is not less than 650 cubic millimeters, the surface area of sample collecting device board and is not less than 50% of sample collecting device total surface area; 20~500 microns of the average pore sizes of capillary micropore matrix structure, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of this sample collecting device is 0.21~0.86 gram/cubic centimetre.In this sample collecting device, preferred parameter be hydrophilic fibre to account for sample collecting device general assembly (TW) ratio range be 10%~58%, hydrophilic fibre is preferably cotton fiber, hydrophobic material is preferably polyester; Cotton fiber is preferably 1: 9 to 3: 2 with weight polyester than scope; The average pore size of capillary micropore matrix structure is preferably 20~200 microns, and the total void rate is preferably 40~45%, and the density of sample collecting device 6 is preferably 0.48~0.52 gram/cubic centimetre.
As another kind of selectivity embodiment, described sample collecting device 6 is made by hydrophobic material, can be made by one or more hydrophobic materials, this hydrophobic material forms capillary micropore matrix structure, has the effect that separates voluntarily and carry the target detection thing in the liquid sample.Hydrophobic material can be tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, polyester, nylon, polyethersulfone or other plastics, glass, resin etc.; This sample collecting device profile can be bar-shaped or tabular, its parameter: total surface area is not less than 700 square millimeters, cumulative volume and is not less than 650 cubic millimeters, the surface area of sample collecting device board and is not less than 50% of sample collecting device total surface area; 20~500 microns of the average pore sizes of capillary micropore matrix structure, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of sample collecting device is 0.49~0.92 gram/cubic centimetre.In this sample collecting device, preferred parameter is the hydrophobic material preferred polyester; The average pore size of capillary micropore matrix structure is preferably 20~200 microns, total void rate and is preferably 35~55%, and the density of sample collecting device is preferably 0.58~0.72 gram/cubic centimetre.This hydrophobic material also can be through making sample collecting device after the hydrophilic treatment again, or the sample collecting device that will be made by hydrophobic material is through hydrophilic treatment, with the ability that improves the sample collecting device collection and carry fecal sample.
Above-mentioned sample collecting device 6 is controlled below 500 cubic millimeters by the amount of its capillary micropore matrix structure saturated absorption liquid sample, time with immersion way saturated absorption liquid sample was controlled in 5 minutes, in preferred 1 minute, behind the sample collecting device saturated absorption liquid, its inner structure requires not take place significant change, especially after super-dry was handled, tangible change did not take place in its rate of water absorption.Under the prerequisite that does not have extraneous mechanical compress or filtration, the liquid sample that is adsorbed in the capillary micropore matrix structure runs to the time control at shank 62 edges in 10 minutes, preferably in 2 minutes from board 64 edges.
Also comprise developping agent (not shown), be used with this RV-ADV antigen ight soil fast detecting instrument, can make an addition on the sample collecting device 6 that this developping agent splendid attire is in the transparent rigid plastic bottle of sealing.Described developping agent is different from damping fluids such as laboratory PBS commonly used or Tris, it is the composite buffer system of a kind of pH value between 6.5~8.5, it act as the pH value of regulating fecal sample, make it to meet the potential of hydrogen needs that RV-ADV antigen immune chromatography reagent strip 4 detects RV and/or ADV antigen, guarantee directivity and flow velocity that fecal sample is carried, filling up because of fecal sample carries the back in space that sample collecting device stays to RV-ADV antigen immune chromatography reagent strip 4 directions, the RV and/or the ADV antigen that can also wash-out adhere to sample collecting device 6 capillary micropore matrix structure surfaces, and it is flowed to RV-ADV antigen immune chromatography reagent strip 4 directions with developping agent, thereby make RV-ADV antigen immune chromatography reagent strip 4 obtain enough fecal samples, and the RV antigen in the promotion fecal sample, ADV antigen or RV separate voluntarily with ADV antigen and carry, effectively improve RV antigen, ADV antigen or RV and the ADV antigen front end concentration in solvent (comprising fecal sample and developping agent), assurance detects the needs of RV or ADV antigen.The component of every 1L developping agent is: potassium dihydrogen phosphate 0.2g, trishydroxymethylaminomethane 6.06g, sodium hydrogen phosphate 1.15g, sodium chloride 8.0g, potassium chloride 0.2g, Tween-20 0.5ml, thimerosal 0.1g, lauryl sodium sulfate 1.25g, ethylenediamine tetraacetic acid 1ml, add deionized water dissolving to 1L solution, rigid plastic bottle splendid attire with transparent sealing, every bottle of 0.8~3.0ml, room temperature preservation.
In the present embodiment, the base plate 22 of plastics containment vessel 2 adopts the ultrasonic plastic welder welding to fixedly connected with panel 24.During installation; in the ultrasonic plastic welder mould, lay the base plate 22 of plastics containment vessel 2; assemble RV-ADV antigen immune chromatography reagent strip 4 and sample collecting device 6 successively in base plate 22 inboards; make the shank 62 of sample collecting device 6 and the sample application zone 42 overlapping 1-3 millimeters of RV-ADV antigen immune chromatography reagent strip 4; the panel 24 of plastics containment vessel 2 is installed then; eyeglass 28 is installed in testing result watch window 27 positions at panel 24 middle parts; with the base plate 22 and panel 24 of ultrasonic plastic welder with plastics containment vessel 2; and sealing wire fusion and the fixing welding of the panel 24 of plastics containment vessel 2 and eyeglass 28, thereby form the RV-ADV antigen ight soil fast detecting instrument of an integral body.
During detection, utilize the board 64 of sample collecting device 6 to absorb residual liquid state or the semi liquid state fecal sample of diarrhoea infant crissum, and be transported to the sample application zone 42 of RV-ADV antigen immune chromatography reagent strip 4 by the shank 62 of sample collecting device 6, immunochromatography principle by RV-ADV antigen immune chromatography reagent strip 4 makes RV antigen in the fecal sample, ADV antigen, or RV and ADV antigen are transported to the reaction zone 44 of RV-ADV antigen immune chromatography reagent strip 4, to the RV antigen in the fecal sample, ADV antigen, or RV and ADV antigen carry out fast detecting, a series of biology takes place in this reaction zone 44, chemical reaction, and demonstrate colored band at reaction zone, can read testing result accordingly.
In sum, RV-ADV antigen ight soil fast detecting instrument of the present invention is in conjunction with sample collecting device and RV-ADV antigen immune chromatography reagent strip, effective RV or the ADV antigen in the qualitative detection diarrhoea residual liquid state of infant crissum or the semi liquid state fecal sample, be used for diagnosis diarrhoea infant and whether infect rotavirus or adenovirus, the propagation of RV or ADV is reduced to minimum degree, use access toilet and fecal sample transit link by evading, avoid related personnel, surrounding environment or article are polluted, reduce the new infection sources and occur; In addition, the present invention uses simply, and is easy to operate, is not only applicable to medical institutions the diarrhoea infant is carried out rotavirus or adenovirus infection examination, to diagnose its cause of disease, is applicable to that also individuality or family detect voluntarily, can be used as the standing health detection product of family.
The above for the person of ordinary skill of the art, can make other various corresponding changes and distortion according to technical scheme of the present invention and technical conceive, and all these changes and distortion all should belong to the scope that the present invention protects.

Claims (9)

1. RV-ADV antigen ight soil fast detecting instrument, it is characterized in that, comprise: the plastics containment vessel, place RV-ADV antigen immune chromatography reagent strip in the plastics containment vessel, and sample collecting device, this RV-ADV antigen immune chromatography reagent strip comprises sample application zone, reaction zone and the suction zones that sets gradually; Sample collecting device has capillary micropore matrix structure, the joint portion that comprises shank, board and connection shank and board, this sample collecting device be used for to be gathered diarrhoea infant crissum presence of residual feces sample, and separates voluntarily and carry RV antigen, ADV antigen or RV and ADV antigen in the fecal sample; The panel that the plastics containment vessel comprises base plate and is equipped with base plate, panel is provided with the watch window for the interpretation testing result, the corresponding setting of reaction zone of watch window and RV-ADV antigen immune chromatography reagent strip; The sample application zone top that the shank of sample collecting device is overlapped and is positioned over RV-ADV antigen immune chromatography reagent strip, board stretches out outside the plastics containment vessel; Described RV-ADV antigen immune chromatography reagent strip comprises RV antigen immune chromatography reagent strip, ADV antigen immune chromatography reagent strip, reaches the antigen combined immunochromatography reagent of RV+ADV bar.
2. RV-ADV antigen ight soil fast detecting instrument as claimed in claim 1, it is characterized in that, also comprise developping agent, be used with this RV-ADV antigen ight soil fast detecting instrument, make an addition on the sample collecting device, with the pH value of regulating fecal sample and directivity and the flow velocity that guarantees the fecal sample transportation, make RV-ADV antigen immune chromatography reagent strip obtain enough fecal samples, and promote RV antigen, ADV antigen or RV in the fecal sample to separate voluntarily with ADV antigen and carry; This developping agent splendid attire is in the transparent rigid plastic bottle of sealing.
3. RV-ADV antigen ight soil fast detecting instrument as claimed in claim 2, it is characterized in that, described developping agent is that a kind of pH value is the compound damping fluid of 6.5-8.5, and is formulated in proportion by potassium dihydrogen phosphate, trishydroxymethylaminomethane, sodium hydrogen phosphate, sodium chloride, potassium chloride, Tween-20, thimerosal, lauryl sodium sulfate, ethylenediamine tetraacetic acid, deionized water.
4. RV-ADV antigen ight soil fast detecting instrument as claimed in claim 1, it is characterized in that, described plastics containment vessel one end is made as the holding area, the base plate of plastics containment vessel arranges several air holes in an end of holding area, its inboard is made as the sample collecting device assembly section away from an end of holding area, and the sample collecting device stationary installation is set on it; The middle part is made as RV-ADV antigen immune chromatography reagent strip assembly section, and it is provided with for the stationary installation of placing RV-ADV antigen immune chromatography reagent strip.
5. RV-ADV antigen ight soil fast detecting instrument as claimed in claim 1, it is characterized in that, described watch window top is bonded with an eyeglass, be marked with " T ", " C " or " Tr ", " Ta " on the eyeglass, " C " indicates line, respectively with detection line and the corresponding setting of nature controlling line of RV-ADV antigen immune chromatography reagent strip; Eyeglass is made by transparent rigid plastic.
6. RV-ADV antigen ight soil fast detecting instrument as claimed in claim 1; it is characterized in that; the base plate of described plastics containment vessel and panel inboard arrange coupling arrangement; be used for base plate is fixedlyed connected with panel, fixed connection method adopts the method for withholding or adopts the ultrasonic plastic welder welding fixedly connected.
7. RV-ADV antigen ight soil fast detecting instrument as claimed in claim 1 is characterized in that, described RV-ADV antigen immune chromatography reagent strip adopts colloid gold label, electroselenium mark, color latex particle mark or the preparation of fluorescent-substance markers technology.
8. RV-ADV antigen ight soil fast detecting instrument as claimed in claim 1 is characterized in that described sample collecting device is made by hydrophilic fibre and hydrophobic material, and this hydrophilic fibre and hydrophobic material form capillary micropore matrix structure; It is 8%~80% that hydrophilic fibre accounts for sample collecting device general assembly (TW) ratio range, and hydrophilic fibre is cotton fiber, filter paper, sponge or cloth; Hydrophobic material is tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, nylon and polyethersulfone and other plastics, glass or resin; The weight ratio scope of hydrophilic fibre and hydrophobic material is 1: 9 to 4: 1; The profile of sample collecting device is table tennis plate or slurry shape, and its total surface area is not less than 700 square millimeters, and cumulative volume is not less than 650 cubic millimeters, and the surface area of sample collecting device board is not less than sample collecting device total surface area 50%; 20~500 microns of the average pore sizes of capillary micropore matrix, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of sample collecting device is 0.21~0.86 gram/cubic centimetre.
9. RV-ADV antigen ight soil fast detecting instrument as claimed in claim 1 is characterized in that described sample collecting device is made by one or more hydrophobic materials, and this hydrophobic material forms capillary micropore matrix structure; Hydrophobic material is tygon, polypropylene, multi-styrene, high density polyethylene, ultra high molecular polyethylene, Polyvinylidene fluoride, teflon, polyester, nylon, polyethersulfone or other plastics, glass or resin; The sample collecting device profile is bar-shaped or tabular, and its total surface area is not less than 700 square millimeters, and cumulative volume is not less than 650 cubic millimeters, and the surface area of sample collecting device board is not less than 50% of sample collecting device total surface area; 20~500 microns of the average pore sizes of capillary micropore matrix structure, total void volume are no more than 500 cubic millimeters, total void rate and are no more than 80%, and the corresponding density of sample collecting device is 0.49~0.92 gram/cubic centimetre.
CN2011104448297A 2011-12-27 2011-12-27 RV-ADV antigen feces rapid detector Pending CN103185789A (en)

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CN201053964Y (en) * 2007-05-21 2008-04-30 四川省迈克科技有限责任公司 Vaccine protective antibody quick detection reagent kit
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CN101187665A (en) * 2007-12-27 2008-05-28 北京博晖创新光电技术股份有限公司 Enterovirus immunofluorescence chromatographic assay test paper and its preparation method
CN101339192A (en) * 2008-08-28 2009-01-07 河南省农业科学院 Test paper for one-step detection for pig virus diarrhoea disease pathogen
CN201555723U (en) * 2009-06-25 2010-08-18 益思美诠生物科技(上海)有限公司 Device integrating sampling and reacting
CN102053153A (en) * 2010-11-25 2011-05-11 西安微通生物技术有限公司 Dot immuno gold directed infiltration detection kit and application thereof
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Application publication date: 20130703