CN1963519A - Test paper bar for testing colloidal gold of A-group wheel virus antigen - Google Patents
Test paper bar for testing colloidal gold of A-group wheel virus antigen Download PDFInfo
- Publication number
- CN1963519A CN1963519A CN 200510086857 CN200510086857A CN1963519A CN 1963519 A CN1963519 A CN 1963519A CN 200510086857 CN200510086857 CN 200510086857 CN 200510086857 A CN200510086857 A CN 200510086857A CN 1963519 A CN1963519 A CN 1963519A
- Authority
- CN
- China
- Prior art keywords
- test strips
- group
- virus antigen
- monoclonal antibody
- described test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Abstract
This invention provides one test bar, which comprises the following steps: a, covering A set of group wheel virus single clone antibody and IgG two ban NC fiber film; b, comprising glue gold label A set wheel single clone antibody glass fiber film; c, applying film analysis double antibody clamper to test A set wheel virus of feces.
Description
Technical field
The invention belongs to field of biological detection, be specifically related to a kind of A group rotavirus (RV-group A) Detection of antigen test strips and application thereof.
Background technology
For rotavirus (RV) diagnosis of infection, classical way is that virus is separated and paired sera is 4 multiplication height, is still the goldstandard of clinical diagnosis so far.The RV diagnosis of infection that develops into of modern biotechnology provides many new detection methods, as: Electronic Speculum or immuno-electron microscope detect colyliform virion antigen from stool extract; Use enzyme linked immunosorbent assay (ELISA) and Nucleic Acid Probe Technique and detect virion and antigen; Employing polyacrylamide gel electrophoresis (PAGE) can directly detect the viral RNA in the stool extract; Using RT-polymerase chain reaction (RT-PCR) detects wheel virus antigen etc.But above method all exists complicated operation, and the time is long, needs expensive instrument, equipment, needs the professional to carry out in laboratory with good conditionsi etc., is not suitable for epidemiology survey and existing field boundary detects.
Summary of the invention
(1), the technical matters that will solve
The purpose of this invention is to provide a kind of test strips that detects the A group wheel virus antigen quickly and easily.
Further object of the present invention provides the application of described test strips in detecting the A group wheel virus antigen.
(2), technical scheme
The present invention is a kind of test strip, and it comprises:
(1) bag is by the nitrocellulose membrane of A group rotavirus monoclonal antibody and two bands of anti-mouse IgG;
(2) contain the glass fibre membrane of colloid gold label A group rotavirus monoclonal antibody.
Test strips of the present invention, it also comprises:
(1) reaction holder;
(2) adsorptive pads;
(3) golden labeling antibody diaphragm.
Test strips of the present invention is wherein reacted holder and is selected the PVC plate for use.
Test strips of the present invention, wherein adsorptive pads is selected filter paper for oil for use.
Test strips of the present invention, wherein golden labeling antibody diaphragm has absorbent function simultaneously, selects polyester film, glass fibre or filter paper fibre for use.
The invention also discloses the application (see figure 2) of described test strips in detecting the A group wheel virus antigen.Leave and take patient's ight soil, put into the bottle that dilution is housed, test strips " 4 " is located to insert in the bottle (can take out test strips after observation finishes fruit), the liquid in the sample picks up up, 10-15 minute sentence read result:
As contain the A group wheel virus antigen, then with test strips on the A group rotavirus monoclonal antibody of colloid gold label form corresponding compound, up be coated on nitrocellulose membrane on A group rotavirus monoclonal antibody combine, form red lines, promptly locate to form red stripes at " T ".
No matter whether contain corresponding antigen, the colloid gold label monoclonal antibody continues upwards to creep and the anti-mouse IgG that is coated on the film forms the red precipitate line, promptly locates to form red stripes at " C ".This line is a nature controlling line, loses efficacy as collaurum, and this line just can not occur, and illustrates that test strips lost efficacy.
2 red precipitate lines can appear in positive findings, and 1 red precipitate line appears in negative findings, lose efficacy as lines explanation test strips not occurring.
Technical scheme of the present invention is: the A group rotavirus monoclonal antibody and the anti-mouse IgG difference solid phase (NC film) on nitrocellulose membrane that adopt purifying, the A group rotavirus monoclonal antibody of association colloid gold mark is used rete and is analysed the principle of double antibody sandwich method and detect A group rotavirus in the ight soil.
(3), beneficial effect
Detect the A group wheel virus antigen with test strips of the present invention, easily and fast, simple and direct, do not need special instruments and equipment, do not need professional training, the result is clear easily to be distinguished; Simple to operate, be easy to promote, be fit to basic unit, be suitable for mass detection, be fit to epidemiology survey, the auxiliary detection effect is played in the diagnosis of the infantile diarrhea that the A group rotavirus is caused.
Description of drawings
Fig. 1: the front schematic view of A test strips of the present invention; The side schematic view of B test strips of the present invention; 1: adsorptive pads; 2: (T: bag is by the band of A group rotavirus monoclonal antibody for nitrocellulose membrane; C: bag is by the Quality Control band of anti-mouse IgG); 3: the glass fibre membrane that contains colloid gold label A group rotavirus monoclonal antibody; 4: golden labeling antibody diaphragm; 5: the reaction holder.
Fig. 2: testing result synoptic diagram; Be followed successively by from left to right: two line colour developings of T, C are positive; Line colour developing of C is negative; Two lines of T, C do not develop the color for invalid.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment 1 A group wheel virus antigen test strip (referring to Fig. 1)
The reaction holder is 6.5cm * 0.4cm PCV plate; Adsorptive pads is the filter paper for oil of 2cm * 0.4cm; 1.8cm the nitrocellulose membrane of * 0.4cm wraps successively by anti-mouse IgG, A group rotavirus monoclonal antibody; The A group rotavirus monoclonal antibody glass fibre membrane that contains 0.4cm * 0.4cm colloid gold label; Gold labeling antibody diaphragm is the polyester film of 2.7cm * 0.4cm; Promptly formed A group wheel virus antigen test strip.
Embodiment 2 A group wheel virus antigen test strip (referring to Fig. 1)
The reaction holder is 6.5cm * 0.4cm PCV plate; Adsorptive pads is the filter paper for oil of 2cm * 0.4cm; 1.8cm the nitrocellulose membrane of * 0.4cm wraps successively by anti-mouse IgG, A group rotavirus monoclonal antibody; The A group rotavirus monoclonal antibody glass fibre membrane that contains 0.4cm * 0.4cm colloid gold label; Gold labeling antibody diaphragm is the glass fibre of 2.7cm * 0.4cm; Promptly formed A group wheel virus antigen test strip.
Embodiment 3 A group wheel virus antigen test strip (referring to Fig. 1)
The reaction holder is 6.5cm * 0.4cm PCV plate; Adsorptive pads is the filter paper for oil of 2cm * 0.4cm; 1.8cm the nitrocellulose membrane of * 0.4cm wraps successively by anti-mouse IgG, A group rotavirus monoclonal antibody; The A group rotavirus monoclonal antibody glass fibre membrane that contains 0.4cm * 0.4cm colloid gold label; Gold labeling antibody diaphragm is the filter paper fibre of 2.7cm * 0.4cm; Promptly formed A group wheel virus antigen test strip.
Embodiment 4 detection methods (referring to Fig. 2)
Leave and take patient's ight soil, put into the bottle that dilution is housed, embodiment 1 test strips " 4 " is located to insert in the bottle (can take out test strips after observation finishes fruit), the liquid in the sample picks up up, 10-15 minute sentence read result:
As contain the A group wheel virus antigen, then with test strips on the A group rotavirus monoclonal antibody of colloid gold label form corresponding compound, up be coated on nitrocellulose membrane on A group rotavirus monoclonal antibody combine, form red lines, promptly locate to form red stripes at " T ".
No matter whether contain corresponding antigen, the colloid gold label monoclonal antibody continues upwards to creep and the anti-mouse IgG that is coated on the film forms the red precipitate line, promptly locates to form red stripes at " C ".This line is a nature controlling line, loses efficacy as collaurum, and this line just can not occur, and illustrates that test strips lost efficacy.
2 red precipitate lines can appear in positive findings, and 1 red precipitate line appears in negative findings, lose efficacy as lines explanation test strips not occurring.
Experimental example 1 clinical detection result
186 examples suffer from diarrhoea infant patient stool sample after testing, and the A group rotavirus infects, and two bands, 92 examples occur, accounts for 49.5%.Test strips method (T2) is compared with ELISA (T1a) and PAGE (T1b) method used at present, and sensitivity is 99%, specificity 100%.
ELISA (T1a) and PAGE (T1b) method and test strips method (T2) contrast experiment statistical form
| Positive | Negative | |
| T1a | 93 | 93 |
| T1b | 93 | 93 |
| T2 | 92 | 93 |
| Sensitivity 99% | Specificity 100% |
Claims (6)
1, a kind of test strip is characterized in that, it comprises:
(1) bag is by the nitrocellulose membrane of A group rotavirus monoclonal antibody and two bands of anti-mouse IgG;
(2) contain the glass fibre membrane of colloid gold label A group rotavirus monoclonal antibody.
2, according to the described test strips of claim 1, it also comprises:
(1) reaction holder;
(2) adsorptive pads;
(3) golden labeling antibody diaphragm.
3,, wherein react holder and select the PVC plate for use according to the described test strips of claim 2.
4, according to the described test strips of claim 2, wherein adsorptive pads is selected filter paper for oil for use.
5, according to the described test strips of claim 2, wherein golden labeling antibody diaphragm is selected polyester film, glass fibre or filter paper fibre for use.
6, the application of the arbitrary described test strips of claim 1-5 in detecting the A group wheel virus antigen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510086857 CN1963519A (en) | 2005-11-10 | 2005-11-10 | Test paper bar for testing colloidal gold of A-group wheel virus antigen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510086857 CN1963519A (en) | 2005-11-10 | 2005-11-10 | Test paper bar for testing colloidal gold of A-group wheel virus antigen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1963519A true CN1963519A (en) | 2007-05-16 |
Family
ID=38082661
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200510086857 Pending CN1963519A (en) | 2005-11-10 | 2005-11-10 | Test paper bar for testing colloidal gold of A-group wheel virus antigen |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1963519A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101187665B (en) * | 2007-12-27 | 2011-08-10 | 北京博晖创新光电技术股份有限公司 | Enterovirus immunofluorescence chromatographic assay test paper and its preparation method |
| CN101498730B (en) * | 2009-03-16 | 2013-06-26 | 深圳市菲鹏生物股份有限公司 | Improved double-antigen sandwiching immunity detection method |
| CN103185789A (en) * | 2011-12-27 | 2013-07-03 | 深圳市爱速尔生物技术有限公司 | RV-ADV antigen feces rapid detector |
| CN103901198A (en) * | 2012-12-26 | 2014-07-02 | 深圳先进技术研究院 | Immune test paper for detecting group A rotaviruses, and its making method |
| CN104502608A (en) * | 2014-12-23 | 2015-04-08 | 北京出入境检验检疫局检验检疫技术中心 | Group A rotavirus chromatography test paper strip based on low-noise excitation fluorescent label |
| CN110018313A (en) * | 2019-04-10 | 2019-07-16 | 江苏硕世生物科技股份有限公司 | A kind of A group wheel virus antigen test strip, kit and preparation method thereof |
-
2005
- 2005-11-10 CN CN 200510086857 patent/CN1963519A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101187665B (en) * | 2007-12-27 | 2011-08-10 | 北京博晖创新光电技术股份有限公司 | Enterovirus immunofluorescence chromatographic assay test paper and its preparation method |
| CN101498730B (en) * | 2009-03-16 | 2013-06-26 | 深圳市菲鹏生物股份有限公司 | Improved double-antigen sandwiching immunity detection method |
| CN103185789A (en) * | 2011-12-27 | 2013-07-03 | 深圳市爱速尔生物技术有限公司 | RV-ADV antigen feces rapid detector |
| CN103901198A (en) * | 2012-12-26 | 2014-07-02 | 深圳先进技术研究院 | Immune test paper for detecting group A rotaviruses, and its making method |
| CN104502608A (en) * | 2014-12-23 | 2015-04-08 | 北京出入境检验检疫局检验检疫技术中心 | Group A rotavirus chromatography test paper strip based on low-noise excitation fluorescent label |
| CN110018313A (en) * | 2019-04-10 | 2019-07-16 | 江苏硕世生物科技股份有限公司 | A kind of A group wheel virus antigen test strip, kit and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112730851B (en) | Detection method and detection kit for high-sensitivity SARS-CoV-2 neutralizing antibody | |
| CN211148669U (en) | S-antigen-based novel coronavirus 2019-nCoV antibody rapid detection kit | |
| CN1963519A (en) | Test paper bar for testing colloidal gold of A-group wheel virus antigen | |
| CN111337669A (en) | Novel coronavirus detection test paper and detection method for rapid detection | |
| CN111239400A (en) | Colloidal gold immunochromatographic device for detecting COVID-19 and use method thereof | |
| CN111999492B (en) | Colloidal gold immunochromatography detection card for combined detection of COVID-19N antigen and S protein antibody | |
| CN113533721B (en) | Colloidal gold method detection test strip for influenza A/B virus antigen and preparation method thereof | |
| CN101701958A (en) | Fluorescent test paper strip capable of simultaneously testing newcastle disease and bird flu virus and application thereof | |
| WO2011008030A2 (en) | Chromatographic system for nucleic acid detection | |
| CN104726606B (en) | A kind of enzyme-linked double cross method detection the pathogenic microorganism examination methods of PCR | |
| CN1963510A (en) | Test paper bar for testing colloidal gold of F1 antibody of plague bacterium | |
| CN1904615A (en) | Influenza A,B virus antigen colloidal gold combined detection test paper | |
| EP2270508A1 (en) | Membrane assay method and kit | |
| CN211339511U (en) | Integrated test strip for LAMP or RT-LAMP | |
| CN1963518A (en) | Test paper bar for testing colloidal gold of 0157: H7 bacillus coli antigen | |
| JP3628709B2 (en) | Test kit and its use | |
| CN1963521A (en) | Rapid test paper bar for testing colloidal gold of 01 group cholera vibrio | |
| CN1963509A (en) | Test paper bar for testing colloidal gold of protective antibody of rabies virus | |
| CN113281504B (en) | Novel immunochromatography detection device | |
| CN101363866B (en) | Test paper strip for detecting encephalitis virus IgM antibody colloidal gold, method for making same and applications | |
| CN114076825A (en) | Detection reagent strip, kit and method for detecting SARS-Cov-2 virus | |
| CN1963520A (en) | Rapid test paper bar for testing colloidal gold of 0139 group cholera vibrio | |
| CN1963514A (en) | Test paper bar for testing colloidal gold of capsular antibody of Bacillus anthracis | |
| CN1963508A (en) | Test paper bar for testing colloidal gold of capsular antibody of Bacillus anthracis | |
| CN105929175A (en) | Semi-quantitative detection colloidal gold test paper for cystatin C in urine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Open date: 20070516 |