CN1963521A - Rapid test paper bar for testing colloidal gold of 01 group cholera vibrio - Google Patents
Rapid test paper bar for testing colloidal gold of 01 group cholera vibrio Download PDFInfo
- Publication number
- CN1963521A CN1963521A CNA200510086859XA CN200510086859A CN1963521A CN 1963521 A CN1963521 A CN 1963521A CN A200510086859X A CNA200510086859X A CN A200510086859XA CN 200510086859 A CN200510086859 A CN 200510086859A CN 1963521 A CN1963521 A CN 1963521A
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- CN
- China
- Prior art keywords
- cholera vibrio
- antigen
- group cholera
- test strips
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
This invention provides one rapid test bar for 01 cholera vibrio antigen, which covers 01 cholera vibrio An antigen single clone antibody or multiple clone antibody and quality IgG on NC film and combines glue gold label single clone antigen and applies film analysis double antibody clamper and tests the cholera vibrio antigen.
Description
Technical field
The invention belongs to field of biological detection, be specifically related to a kind of O1 group cholera vibrio Detection of antigen test strips and application thereof.
Background technology
Cholera is that its characteristic feature is to be lost in a large number by body fluid and electrolyte that serious diarrhoea causes by a kind of acute infectious disease due to the comma bacillus infection body.Comma bacillus can be divided into more than 200 sero-group according to the specificity of its somatic antigen, and wherein the most common also to be that tool is communicable be O1 group.
At present the traditional detection method of O1 group cholera vibrio be direct separation and Culture and increase bacterium after separation and Culture etc., complicated operation, need the professional and technical personnel to carry out in specialized laboratory, time-consumingly reach 16-24 hour, can't handle a large amount of suspicious samples, therefore, for the detection of O1 group cholera vibrio, far do not reach to meet and rush down the requirement that to examine, on detection time, do not reach the purpose of rapid screening simultaneously yet.As PCR, latex agglutination test and monoclonal antibody diazo emulsion test (DPRC), though they can both diagnose out comma bacillus in several hours, has higher specificity and susceptibility, but simultaneously they exist various defective again, require relatively costliness etc. of high, equipment as: technical conditions.At the popular present situation of O1 group cholera vibrio and control requirement, for the diagnosis of cholera, not only demanding specificity and susceptibility also need fast, easy, are suitable for primary care and health unit and use.
Summary of the invention
(1), the technical matters that will solve
The purpose of invention provides a kind of easy to use, quick, is used to detect the test strips of O1 group cholera vibrio, detects O1 group cholera vibrio A antigen in the human feces, is used for the auxiliary diagnosis of O1 group cholera vibrio.
(2), technical scheme
The invention provides a kind of test strip, it comprises following composition:
(1) wraps simultaneously by the nitrocellulose membrane of the monoclonal antibody of O1 group cholera vibrio A antigen or polyclonal antibody and two bands of the anti-mouse IgG of Quality Control;
(2) contain the glass fibre membrane of the monoclonal antibody of colloid gold label O1 group cholera vibrio A antigen.
Preferred test strips of the present invention also comprises:
(1) reaction holder;
(2) adsorptive pads;
(3) golden labeling antibody diaphragm.
Wherein react the preferred PVC plate of holder; The preferred filter paper for oil of adsorptive pads; Gold labeling antibody diaphragm has absorbent function simultaneously, preferably uses polyester film, glass fibre or filter paper fibre.
The invention also discloses the application of described test strips in detecting the O1 group cholera vibrio.
Technical scheme of the present invention is: the monoclonal antibody or polyclonal antibody and the anti-mouse IgG difference solid phase (NC film) on nitrocellulose membrane that adopt the O1 group cholera vibrio A antigen of purifying, the monoclonal antibody of the O1 group cholera vibrio A antigen of association colloid gold mark is used rete and is analysed the principle of double antibody sandwich method and detect O1 group cholera vibrio in the sample.
(3), beneficial effect
Test strips of the present invention utilizes colloidal gold-labeled method and rete to analyse technology, is used for the comma bacillus that fast qualitative half-quantitative detection sample may exist, and reaches quick screening patient, in time controls the purpose of epidemic situation.Also can be used for simultaneously the rapid screening after the external environment sample increases bacterium.For next step isolation identification has been created advantage, save a large amount of manpower and materials, easily and fast, simple and direct, do not needed special instruments and equipment, do not need professional training, the result is clear easily to be distinguished, simple to operate, is easy to promote, be fit to basic unit, the mass field that is suitable for accident detects, and is fit to epidemiology survey, and the Infect And Diagnose of O1 group cholera vibrio is played booster action.
Description of drawings
Fig. 1: the front schematic view of A test strips of the present invention;
The side schematic view of B test strips of the present invention.Wherein,
1: adsorptive pads;
2: (T: bag is by the monoclonal antibody of O1 group cholera vibrio A antigen or the band of polyclonal antibody for nitrocellulose membrane; C: bag is by the Quality Control band of anti-mouse IgG);
3: the glass fibre membrane that contains the monoclonal antibody of colloid gold label O1 group cholera vibrio A antigen;
4: golden labeling antibody diaphragm;
5: the reaction holder.
Fig. 2: testing result synoptic diagram.Wherein,
Be followed successively by from left to right: two line colour developings of T, C are positive; Line colour developing of C is negative; Two lines of T, C all do not develop the color for invalid.
Embodiment
Can further be expressly understood the present invention by specific embodiments of the invention given below and experimental example, but not be limitation of the invention.
Embodiment 1 O1 group cholera vibrio colloidal gold fast detecting test paper strip (referring to Fig. 1)
The reaction holder is 6.5cm * 0.4cm PCV plate; Adsorptive pads is the filter paper for oil of 2cm * 0.4cm; 1.8cm the nitrocellulose membrane of * 0.4cm wraps successively by anti-mouse IgG, the monoclonal antibody or the polyclonal antibody of O1 group cholera vibrio A antigen contain the monoclonal antibody glass fibre membrane of the O1 group cholera vibrio A antigen of 0.4cm * 0.4cm colloid gold label; Gold labeling antibody diaphragm is the polyester film of 2.7cm * 0.4cm; Promptly formed O1 group cholera vibrio colloidal gold fast detecting test paper strip.
Embodiment 2:O1 group cholera vibrio colloidal gold fast detecting test paper strip (referring to Fig. 1)
The reaction holder is 6.5cm * 0.4cm PCV plate; Adsorptive pads is the filter paper for oil of 2cm * 0.4cm; 1.8cm the nitrocellulose membrane of * 0.4cm wraps successively by anti-mouse IgG, the monoclonal antibody or the polyclonal antibody of O1 group cholera vibrio A antigen contain the monoclonal antibody glass fibre membrane of the O1 group cholera vibrio A antigen of 0.4cm * 0.4cm colloid gold label; Gold labeling antibody diaphragm is the glass fibre of 2.7cm * 0.4cm; Promptly formed O1 group cholera vibrio colloidal gold fast detecting test paper strip.
Embodiment 3 O1 group cholera vibrio colloidal gold fast detecting test paper strips (referring to Fig. 1)
The reaction holder is 6.5cm * 0.4cm PCV plate; Adsorptive pads is the filter paper for oil of 2cm * 0.4cm; 1.8cm the nitrocellulose membrane of * 0.4cm wraps successively by anti-mouse IgG, the monoclonal antibody or the polyclonal antibody of O1 group cholera vibrio A antigen contain the monoclonal antibody glass fibre membrane of the O1 group cholera vibrio A antigen of 0.4cm * 0.4cm colloid gold label; Gold labeling antibody diaphragm is the filter paper fibre of 2.7cm * 0.4cm; Promptly formed O1 group cholera vibrio colloidal gold fast detecting test paper strip.
Embodiment 4 detection methods (referring to Fig. 2)
Sample is put into the bottle that dilution is housed, embodiment 1 test strips " 4 " is located to insert in the bottle (can take out test strips after observation finishes fruit), the liquid in the sample picks up up, interpretation in 10-15 minute.
The result:
As contain O1 group cholera vibrio antigen, then with test strips on the monoclonal antibody of O1 group cholera vibrio A antigen of colloid gold label form corresponding compound, up be coated on the monoclonal antibody or the polyclonal antibody of the O1 group cholera vibrio A antigen on the nitrocellulose membrane, form red lines, promptly form red stripes at the T place.
No matter whether contain corresponding antigen, the monoclonal antibody of colloid gold label O1 group cholera vibrio A antigen continues upwards to creep and the anti-mouse IgG that is coated on the film forms the red precipitate line, promptly locates to form red stripes at " C ".This line is a nature controlling line, loses efficacy as collaurum, and this line just can not occur, and illustrates that test strips lost efficacy.
The experimental example clinical samples detects
Certain province disease prevention and control center checks diarrhoea patient's sample 400 examples, wherein positive sample 50 examples in epidemic season, non-positive sample 350 examples, compare with traditional microbe growth (T1) result, in the 50 routine positive sample, test strips (T2) has an example not detect as a result.Its susceptibility is 98%, and specificity is 100%.The results are shown in following table.
Bacteria cultivation results (T1) and test strips method (T2) contrast experiment statistical form
| Positive | Negative | |
| T1 | 50 | 350 |
| T2 | 49 | 350 |
| Sensitivity 98% | Specificity is 100% |
Claims (6)
1, a kind of test strip is characterized in that, it comprises:
(1) wraps simultaneously by the nitrocellulose membrane of the monoclonal antibody of O1 group cholera vibrio A antigen or polyclonal antibody and two bands of the anti-mouse IgG of Quality Control;
(2) contain the glass fibre membrane of the monoclonal antibody of colloid gold label O1 group cholera vibrio A antigen.
2, according to the described test strips of claim 1, it also comprises:
(1) reaction holder;
(2) adsorptive pads;
(3) golden labeling antibody diaphragm.
3,, wherein react holder and select the PVC plate for use according to the described test strips of claim 2.
4, according to the described test strips of claim 2, wherein adsorptive pads is selected filter paper for oil for use.
5, according to the described test strips of claim 2, wherein golden labeling antibody diaphragm is selected polyester film, glass fibre or filter paper fibre for use.
6, the application of the arbitrary described test strips of claim 1-5 in detecting the O1 group cholera vibrio.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200510086859XA CN1963521A (en) | 2005-11-10 | 2005-11-10 | Rapid test paper bar for testing colloidal gold of 01 group cholera vibrio |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200510086859XA CN1963521A (en) | 2005-11-10 | 2005-11-10 | Rapid test paper bar for testing colloidal gold of 01 group cholera vibrio |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1963521A true CN1963521A (en) | 2007-05-16 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200510086859XA Pending CN1963521A (en) | 2005-11-10 | 2005-11-10 | Rapid test paper bar for testing colloidal gold of 01 group cholera vibrio |
Country Status (1)
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| CN (1) | CN1963521A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101968487A (en) * | 2009-03-31 | 2011-02-09 | 东北农业大学 | Immune colloidal gold test paper for detecting listeria hemolysin and preparation method thereof |
| CN104502598A (en) * | 2014-12-23 | 2015-04-08 | 北京出入境检验检疫局检验检疫技术中心 | Fluorescent mark-based immunochromatographic test strip for vibrio cholerae O1 serotype ogawa |
| CN106290841A (en) * | 2015-05-13 | 2017-01-04 | 上海凯创生物技术有限公司 | Vibrio cholerae O 1 emulsion technique detection kit |
| CN106290845A (en) * | 2015-05-13 | 2017-01-04 | 上海凯创生物技术有限公司 | Vibrio cholerae O 1 colloidal gold method detection kit |
-
2005
- 2005-11-10 CN CNA200510086859XA patent/CN1963521A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101968487A (en) * | 2009-03-31 | 2011-02-09 | 东北农业大学 | Immune colloidal gold test paper for detecting listeria hemolysin and preparation method thereof |
| CN104502598A (en) * | 2014-12-23 | 2015-04-08 | 北京出入境检验检疫局检验检疫技术中心 | Fluorescent mark-based immunochromatographic test strip for vibrio cholerae O1 serotype ogawa |
| CN106290841A (en) * | 2015-05-13 | 2017-01-04 | 上海凯创生物技术有限公司 | Vibrio cholerae O 1 emulsion technique detection kit |
| CN106290845A (en) * | 2015-05-13 | 2017-01-04 | 上海凯创生物技术有限公司 | Vibrio cholerae O 1 colloidal gold method detection kit |
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Open date: 20070516 |