CN103173399B - Complex microbial agent and production method thereof - Google Patents
Complex microbial agent and production method thereof Download PDFInfo
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- CN103173399B CN103173399B CN201310125401.5A CN201310125401A CN103173399B CN 103173399 B CN103173399 B CN 103173399B CN 201310125401 A CN201310125401 A CN 201310125401A CN 103173399 B CN103173399 B CN 103173399B
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Abstract
The invention provides a complex microbial agent and a production method thereof. The technical scheme disclosed by the invention comprises the steps of orderly inoculating the same volume of bacillus subtilis bacteria liquid, yeast solution, and lactobacillus bacterial liquid into a fermentation tank, firstly, carrying out aerobic fermentation cultivation, and then carrying out anaerobic fermentation cultivation, so as to obtain the complex microbial agent, wherein the single bacteria number of bacillus subtilis, saccharomycetes and lactic acid bacteria is 106-107 per mL in the complex microbial agent; and the total bacteria number is 107-108 per mL. The cheap and excellent complex microbial agent is provided by the invention; and the cheap and excellent complex microbial agent can be applied to the fields of fast fermentation of farmyard manure, restraint of propagation of malignant bacteria in soil, decomposition of sulfide in aquaculture water and the like, is simple in production method and easy to operate, and can achieve commercialized popularization.
Description
Technical field
The present invention relates to complex micro organism fungicide field, refer to especially a kind of a kind of complex micro organism fungicide and production method thereof that can be used for quick fermentation farm manure.
Background technology
Along with the development of agriculture production and a large amount of uses of chemical fertilizer, soil physical chemistry degradation, environmental pollution phenomenon increase the weight of.Farm manure is with its green, environmental protection, nuisanceless being widely used, but farm manure complicated component, decompose slow, fertilizer efficiency late, be difficult for becoming thoroughly decomposed, in order to overcome these drawbacks, scientist makes great efforts to explore the decomposition rate that improves farm manure always.
Summary of the invention
The present invention proposes a kind of complex micro organism fungicide and production method thereof, decompose problem slow, that be difficult for becoming thoroughly decomposed in order to solve farm manure.
A kind of complex micro organism fungicide, active ingredient comprises: subtilis, yeast and milk-acid bacteria; The single bacterial classification quantity 10 of subtilis, yeast and milk-acid bacteria
6～10
7individual/mL, total count amount 10
7～10
8individual/mL.
The invention allows for a kind of method of producing complex micro organism fungicide, produce 100L complex micro organism fungicide, comprise following production stage:
Step 1: fermented pretreatment, comprises equipment sterilizing, medium sterilization and liquid medium preparation:
Equipment sterilizing: by fermentor tank and 100～110 DEG C of sterilizing 30min～60min of culture medium vessel high temperature, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 DEG C;
Medium sterilization: substratum comprises two kinds of low sugar culture-medium and high glucose mediums, and low sugar culture-medium and high glucose medium are put into pressure kettle sterilizing 1h～3h;
Preparation liquid medium: the low sugar culture-medium after sterilizing and high glucose medium are mixed with 1L sterilized water respectively, put into culture medium vessel, stir 30min for subsequent use;
Step 2: fermentation culture, comprises aerobic cultivation and anaerobism cultivation:
The low sugar liquid medium configuring is drained in fermentor tank, afterwards subtilis bacterium liquid, yeast bacterium liquid and the lactic acid bacterial liquid of cultivating the same volume having activated is inoculated in fermentor tank in order successively, the volume of three kinds of bacterium liquid is 4～7L; Open filter air pump, oxygen is carried out aerobic cultivation for 3～4 days;
In the time of PH=5～6, close filter air pump, afterwards by high glucose medium liquid drainage to fermentor tank, by equipment self-circulation rly. be adjusted to carry out for 12h/ time anaerobism cultivate 3～4 days;
Step 3: fermentation aftertreatment: in the time of PH=3～4, turn off self-circulation rly..
As the preferred technical scheme of one, low sugar culture-medium comprises the component of following weight: brown sugar 0.2～0.3g, peptone 0.06～0.12g, potassium primary phosphate 0.1～0.3g, magnesium sulfate 0.01～0.03g, Repone K 0.03～0.05g, sodium-chlor 0.03～0.05g, yeast extract 0.3～0.5g, halogen sheet 0.01～0.02g, W-Gum 0.1～0.3g.
As the preferred technical scheme of one, high glucose medium comprises the component of following weight: glucose 0.2～0.5g, brown sugar 0.4～0.8g, molasses 0.1～0.3g, yeast extract 0.2～0.5g, urea 0.01～0.03g, vitamins C 0.01～0.03g.
As the preferred technical scheme of one, in subtilis bacterium liquid, the weight ratio of subtilis is 30～40%, and in yeast bacterium liquid, saccharomycetic weight ratio is 20～30%, and in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30～40%.
As the improvement to technique scheme, in subtilis bacterium liquid, the weight ratio of subtilis is 30%, and in described yeast bacterium liquid, saccharomycetic weight ratio is 30%, and in described lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30%.
The invention provides a kind of complex micro organism fungicide inexpensive, of fine quality, can be for the field such as breeding, the decomposition of Sulfide in water used for aquiculture of unwanted bacteria in quick fermentation farm manure, supression soil, production method is easy, easy to operate, can realize commercialization and promote.
Brief description of the drawings
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the Production Flow Chart schematic diagram of a kind of complex micro organism fungicide of proposing of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiment.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
The present invention proposes a kind of complex micro organism fungicide, active ingredient comprises: subtilis, yeast and milk-acid bacteria; The single bacterial classification quantity 10 of subtilis, yeast and milk-acid bacteria
6～10
7individual/mL, total count amount 10
7～10
8individual/mL.
As shown in Figure 1: the invention allows for a kind of method of producing complex micro organism fungicide, produce 100L complex micro organism fungicide, comprise following production stage:
Step 1: fermented pretreatment, comprises equipment sterilizing, medium sterilization and liquid medium preparation:
Equipment sterilizing: by fermentor tank and 100～110 DEG C of sterilizing 30min～60min of culture medium vessel high temperature, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 DEG C;
Medium sterilization: substratum comprises two kinds of low sugar culture-medium and high glucose mediums, and low sugar culture-medium and high glucose medium are put into pressure kettle sterilizing 1h～3h;
Preparation liquid medium: the low sugar culture-medium after sterilizing and high glucose medium are mixed with 1L sterilized water respectively, put into culture medium vessel, stir 30min for subsequent use;
Step 2: fermentation culture, comprises aerobic cultivation and anaerobism cultivation:
The low sugar liquid medium configuring is drained in fermentor tank, afterwards subtilis bacterium liquid, yeast bacterium liquid and the lactic acid bacterial liquid of cultivating the same volume having activated is inoculated in fermentor tank in order successively, the volume of three kinds of bacterium liquid is 4～7L; Open filter air pump, oxygen is carried out aerobic cultivation for 3～4 days;
In the time of PH=5～6, close filter air pump, afterwards by high glucose medium liquid drainage to fermentor tank, by equipment self-circulation rly. be adjusted to carry out for 12h/ time anaerobism cultivate 3～4 days;
Step 3: fermentation aftertreatment: in the time of PH=3～4, turn off self-circulation rly..
Preferably, low sugar culture-medium comprises the component of following weight: brown sugar 0.2～0.3g, peptone 0.06～0.12g, potassium primary phosphate 0.1～0.3g, magnesium sulfate 0.01～0.03g, Repone K 0.03～0.05g, sodium-chlor 0.03～0.05g, yeast extract 0.3～0.5g, halogen sheet 0.01～0.02g, W-Gum 0.1～0.3g.
Preferably, high glucose medium comprises the component of following weight: glucose 0.2～0.5g, brown sugar 0.4～0.8g, molasses 0.1～0.3g, yeast extract 0.2～0.5g, urea 0.01～0.03g, vitamins C 0.01～0.03g.
Preferably, in subtilis bacterium liquid, the weight ratio of subtilis is 30～40%, and in yeast bacterium liquid, saccharomycetic weight ratio is 20～30%, and in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30～40%.
More preferred, in subtilis bacterium liquid, the weight ratio of subtilis is 30%, and in yeast bacterium liquid, saccharomycetic weight ratio is 30%, and in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30%.
The invention provides a kind of complex micro organism fungicide inexpensive, of fine quality, can be for the field such as breeding, the decomposition of Sulfide in water used for aquiculture of unwanted bacteria in quick fermentation farm manure, supression soil, production method is easy, easy to operate, can realize commercialization and promote.
Embodiment 1:
Step 1: fermented pretreatment, comprises equipment sterilizing, medium sterilization and liquid medium preparation:
Equipment sterilizing: by fermentor tank and 100～110 DEG C of sterilizing 30min～60min of culture medium vessel high temperature, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 DEG C;
Medium sterilization: described substratum comprises two kinds of low sugar culture-medium and high glucose mediums: low sugar culture-medium comprises the component of following weight: brown sugar 0.2g, peptone 0.06g, potassium primary phosphate 0.1g, magnesium sulfate 0.01g, Repone K 0.03g, sodium-chlor 0.03g, yeast extract 0.3g, halogen sheet 0.01g, W-Gum 0.1g; High glucose medium comprises the component of following weight: glucose 0.2g, brown sugar 0.4g, molasses 0.1g, yeast extract 0.2g, urea 0.01g, vitamins C 0.01g; Low sugar culture-medium and high glucose medium are put into pressure kettle sterilizing 1h～3h;
Preparation liquid medium: the low sugar culture-medium after sterilizing and high glucose medium are mixed with 1L sterilized water respectively, put into culture medium vessel, stir 30min for subsequent use;
Step 2: fermentation culture, comprises aerobic cultivation and anaerobism cultivation:
The low sugar liquid medium configuring is drained in fermentor tank, afterwards subtilis bacterium liquid, yeast bacterium liquid and the lactic acid bacterial liquid of cultivating the same volume having activated are inoculated in fermentor tank in order successively, wherein: in subtilis bacterium liquid, the weight ratio of subtilis is 30%, in yeast bacterium liquid, saccharomycetic weight ratio is 20%, in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is that the volume of 30%, three kind of bacterium liquid is 4～7L; Open filter air pump, oxygen is carried out aerobic cultivation for 3～4 days;
In the time of PH=5～6, close filter air pump, afterwards by high glucose medium liquid drainage to fermentor tank, by equipment self-circulation rly. be adjusted to carry out for 12h/ time anaerobism cultivate 3～4 days;
Step 3: fermentation aftertreatment: in the time of PH=3～4, turn off self-circulation rly..
The invention provides a kind of complex micro organism fungicide inexpensive, of fine quality, can be for the field such as breeding, the decomposition of Sulfide in water used for aquiculture of unwanted bacteria in quick fermentation farm manure, supression soil, production method is easy, easy to operate, can realize commercialization and promote.
Embodiment 2:
Step 1: fermented pretreatment, comprises equipment sterilizing, medium sterilization and liquid medium preparation:
Equipment sterilizing: by fermentor tank and 100～110 DEG C of sterilizing 30min～60min of culture medium vessel high temperature, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 DEG C;
Medium sterilization: described substratum comprises two kinds of low sugar culture-medium and high glucose mediums, low sugar culture-medium comprises the component of following weight: brown sugar 0.3g, peptone 0.12g, potassium primary phosphate 0.3g, magnesium sulfate 0.03g, Repone K 0.05g, sodium-chlor 0.05g, yeast extract 0.5g, halogen sheet 0.02g, W-Gum 0.3g; High glucose medium comprises the component of following weight: glucose 0.5g, brown sugar～0.8g, molasses 0.3g, yeast extract 0.5g, urea 0.03g, vitamins C 0.03g.Low sugar culture-medium and high glucose medium are put into pressure kettle sterilizing 1h～3h;
Preparation liquid medium: the low sugar culture-medium after sterilizing and high glucose medium are mixed with 1L sterilized water respectively, put into culture medium vessel, stir 30min for subsequent use;
Step 2: fermentation culture, comprises aerobic cultivation and anaerobism cultivation:
The low sugar liquid medium configuring is drained in fermentor tank, afterwards subtilis bacterium liquid, yeast bacterium liquid and the lactic acid bacterial liquid of cultivating the same volume having activated are inoculated in fermentor tank in order successively, wherein: in subtilis bacterium liquid, the weight ratio of subtilis is 40%, in yeast bacterium liquid, saccharomycetic weight ratio is 30%, in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is that the volume of 40%, three kind of bacterium liquid is 4～7L; Open filter air pump, oxygen is carried out aerobic cultivation for 3～4 days;
In the time of PH=5～6, close filter air pump, afterwards by high glucose medium liquid drainage to fermentor tank, by equipment self-circulation rly. be adjusted to carry out for 12h/ time anaerobism cultivate 3～4 days;
Step 3: fermentation aftertreatment: in the time of PH=3～4, turn off self-circulation rly..
The invention provides a kind of complex micro organism fungicide inexpensive, of fine quality, can be for the field such as breeding, the decomposition of Sulfide in water used for aquiculture of unwanted bacteria in quick fermentation farm manure, supression soil, production method is easy, easy to operate, can realize commercialization and promote.
Embodiment 3:
Step 1: fermented pretreatment, comprises equipment sterilizing, medium sterilization and liquid medium preparation:
Equipment sterilizing: by fermentor tank and 100～110 DEG C of sterilizing 30min～60min of culture medium vessel high temperature, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 DEG C;
Medium sterilization: described substratum comprises two kinds of low sugar culture-medium and high glucose mediums, low sugar culture-medium comprises the component of following weight: brown sugar 0.25g, peptone 0.08g, potassium primary phosphate 0.2g, magnesium sulfate 0.02g, Repone K 0.04g, sodium-chlor 0.04g, yeast extract 0.4g, halogen sheet 0.015g, W-Gum 0.2g; High glucose medium comprises the component of following weight: glucose 0.3g, and brown sugar 0.6g, molasses 0.2g, yeast extract 0.3g, urea 0.02g, vitamins C 0.02g, puts into pressure kettle sterilizing 1h～3h by low sugar culture-medium and high glucose medium;
Preparation liquid medium: the low sugar culture-medium after sterilizing and high glucose medium are mixed with 1L sterilized water respectively, put into culture medium vessel, stir 30min for subsequent use;
Step 2: fermentation culture, comprises aerobic cultivation and anaerobism cultivation:
The low sugar liquid medium configuring is drained in fermentor tank, afterwards subtilis bacterium liquid, yeast bacterium liquid and the lactic acid bacterial liquid of cultivating the same volume having activated are inoculated in fermentor tank in order successively, wherein: in subtilis bacterium liquid, the weight ratio of subtilis is 30%, in yeast bacterium liquid, saccharomycetic weight ratio is 30%, and in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30%;
The volume of three kinds of bacterium liquid is 4～7L; Open filter air pump, oxygen is carried out aerobic cultivation for 3～4 days;
In the time of PH=5～6, close filter air pump, afterwards by high glucose medium liquid drainage to fermentor tank, by equipment self-circulation rly. be adjusted to carry out for 12h/ time anaerobism cultivate 3～4 days;
Step 3: fermentation aftertreatment: in the time of PH=3～4, turn off self-circulation rly..
The invention provides a kind of complex micro organism fungicide inexpensive, of fine quality, can be for the field such as breeding, the decomposition of Sulfide in water used for aquiculture of unwanted bacteria in quick fermentation farm manure, supression soil, production method is easy, easy to operate, can realize commercialization and promote.
These are only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any amendment of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (3)
1. a method of producing complex micro organism fungicide, is characterized in that, produces complex micro organism fungicide described in 100L, and the active ingredient of described complex micro organism fungicide comprises: subtilis, yeast and milk-acid bacteria; The single bacterial classification quantity 10 of subtilis, yeast and milk-acid bacteria
6～10
7individual/mL, total count amount 10
7～10
8individual/mL, comprises following production stage:
Step 1: fermented pretreatment, comprises equipment sterilizing, medium sterilization and liquid medium preparation:
Equipment sterilizing: by fermentor tank and 100～110 DEG C of sterilizing 30min～60min of culture medium vessel, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 DEG C;
Medium sterilization: described substratum comprises two kinds of low sugar culture-medium and high glucose mediums; Described low sugar culture-medium comprises the component of following weight: brown sugar 0.2～0.3g, peptone 0.06～0.12g, potassium primary phosphate 0.1～0.3g, magnesium sulfate 0.01～0.03g, Repone K 0.03～0.05g, sodium-chlor 0.03～0.05g, yeast extract 0.3～0.5g, halogen sheet 0.01～0.02g, W-Gum 0.1～0.3g; Described high glucose medium comprises the component of following weight part: glucose 0.2～0.5g, brown sugar 0.4～0.8g, molasses 0.1～0.3g, yeast extract 0.2～0.5g, urea 0.01～0.03g, vitamins C 0.01～0.03g; Low sugar culture-medium and high glucose medium are put into pressure kettle sterilizing 1h～3h;
Preparation liquid medium: the low sugar culture-medium after sterilizing and high glucose medium are mixed with 1L sterilized water respectively, put into described culture medium vessel, stir 30min for subsequent use;
Step 2: fermentation culture, comprises aerobic cultivation and anaerobism cultivation:
The low sugar liquid medium configuring is drained in fermentor tank, afterwards subtilis bacterium liquid, yeast bacterium liquid and the lactic acid bacterial liquid of cultivating the same volume having activated is inoculated in fermentor tank in order successively, the volume of three kinds of bacterium liquid is 4～7L; Open filter air pump, oxygen is carried out aerobic cultivation for 3～4 days;
In the time of PH=5～6, close described filter air pump, afterwards by high glucose medium liquid drainage to fermentor tank, by equipment self-circulation rly. be adjusted to carry out for 12h/ time anaerobism cultivate 3～4 days;
Step 3: fermentation aftertreatment: in the time of PH=3～4, turn off described self-circulation rly..
2. a kind of method of producing complex micro organism fungicide as claimed in claim 1, it is characterized in that: in described subtilis bacterium liquid, the weight ratio of subtilis is 30～40%, in described yeast bacterium liquid, saccharomycetic weight ratio is 20～30%, and in described lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30～40%.
3. a kind of method of producing complex micro organism fungicide as claimed in claim 1, it is characterized in that, in described subtilis bacterium liquid, the weight ratio of subtilis is 30%, in described yeast bacterium liquid, saccharomycetic weight ratio is 30%, and in described lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30%.
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| CN105274016A (en) * | 2014-06-09 | 2016-01-27 | 济源市国龙生物科技有限公司 | Microbial straw fermentation agent and method for producing straw forage by utilizing fermentation agent |
| CN104726103B (en) * | 2015-03-31 | 2016-03-09 | 山东省林业科学研究院 | A kind of drought mountain microorganism combining bacteria liquid and gel preparation thereof |
| CN104788262A (en) * | 2015-04-10 | 2015-07-22 | 张永松 | Organic liquid fertilizer for C3 type crop as well as a preparation method and application method of organic liquid fertilizer |
| CN104862247A (en) * | 2015-04-30 | 2015-08-26 | 山西师范大学 | Liquid medium of composite microbial agent and preparation method thereof |
| CN105567604A (en) * | 2016-02-16 | 2016-05-11 | 江苏中通生物科技有限公司 | Compound microorganism bacterium agent and preparation method thereof |
| CN106348817B (en) * | 2016-08-26 | 2019-08-09 | 山东省鲁洲食品集团有限公司 | A kind of technique that liquid bio-fertilizer is prepared with corn starch sugar leftover bits and pieces |
| CN107259074A (en) * | 2017-08-11 | 2017-10-20 | 广西农垦西江乳业有限公司奶牛场 | A kind of solid fungicide and its preparation and application for being used to make silage corn |
| CN109943491A (en) * | 2019-04-23 | 2019-06-28 | 漳州旺德生物科技有限公司 | A kind of method for extracting and culturing of composite microbial fermentation bacteria |
| CN111728223A (en) * | 2020-07-07 | 2020-10-02 | 西安因子元素科技有限公司 | Method for producing compound cytokine elements by fermentation |
| CN111978120A (en) * | 2020-08-25 | 2020-11-24 | 湖南启农生物科技有限公司 | Liquid environment-friendly composite microbial fertilizer and preparation method thereof |
| CN113308324B (en) * | 2021-06-01 | 2023-07-25 | 厦门元之道生物科技有限公司 | Preparation method of plant-based low-sugar beverage fermented by natural yeast |
| CN114276186A (en) * | 2021-12-17 | 2022-04-05 | 广东恒懋生物农业科技有限公司 | Efficient biological bacterial fertilizer and preparation method thereof |
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