CN103173399A - Complex microbial agent and production method thereof - Google Patents
Complex microbial agent and production method thereof Download PDFInfo
- Publication number
- CN103173399A CN103173399A CN2013101254015A CN201310125401A CN103173399A CN 103173399 A CN103173399 A CN 103173399A CN 2013101254015 A CN2013101254015 A CN 2013101254015A CN 201310125401 A CN201310125401 A CN 201310125401A CN 103173399 A CN103173399 A CN 103173399A
- Authority
- CN
- China
- Prior art keywords
- medium
- liquid
- subtilis
- culture
- weight ratio
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 16
- 230000000813 microbial effect Effects 0.000 title abstract 6
- 239000007788 liquid Substances 0.000 claims abstract description 77
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims abstract description 62
- 241000894006 Bacteria Species 0.000 claims abstract description 60
- 235000014655 lactic acid Nutrition 0.000 claims abstract description 31
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 22
- 238000000855 fermentation Methods 0.000 claims abstract description 21
- 230000004151 fermentation Effects 0.000 claims abstract description 20
- 230000001580 bacterial effect Effects 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000004310 lactic acid Substances 0.000 claims abstract description 16
- 230000001954 sterilising effect Effects 0.000 claims description 39
- 239000001963 growth medium Substances 0.000 claims description 36
- 238000004659 sterilization and disinfection Methods 0.000 claims description 33
- 239000006481 glucose medium Substances 0.000 claims description 24
- 239000002609 medium Substances 0.000 claims description 24
- 230000000855 fungicidal effect Effects 0.000 claims description 23
- 239000000417 fungicide Substances 0.000 claims description 23
- 244000005700 microbiome Species 0.000 claims description 23
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000012138 yeast extract Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 7
- 229910019142 PO4 Inorganic materials 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 6
- 108010080698 Peptones Proteins 0.000 claims description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 6
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 6
- 230000004913 activation Effects 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 6
- 238000009835 boiling Methods 0.000 claims description 6
- 239000004202 carbamide Substances 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 229910052736 halogen Inorganic materials 0.000 claims description 6
- 150000002367 halogens Chemical class 0.000 claims description 6
- 238000011177 media preparation Methods 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- 235000013379 molasses Nutrition 0.000 claims description 6
- 229910052760 oxygen Inorganic materials 0.000 claims description 6
- 239000001301 oxygen Substances 0.000 claims description 6
- 235000019319 peptone Nutrition 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 6
- 239000010452 phosphate Substances 0.000 claims description 6
- 229910052700 potassium Inorganic materials 0.000 claims description 6
- 239000011591 potassium Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 238000003756 stirring Methods 0.000 claims description 6
- 235000019154 vitamin C Nutrition 0.000 claims description 6
- 239000011718 vitamin C Substances 0.000 claims description 6
- 239000004480 active ingredient Substances 0.000 claims description 3
- 210000003608 fece Anatomy 0.000 abstract description 11
- 239000010871 livestock manure Substances 0.000 abstract description 11
- 238000000354 decomposition reaction Methods 0.000 abstract description 7
- 239000002689 soil Substances 0.000 abstract description 7
- 238000009360 aquaculture Methods 0.000 abstract description 6
- 239000003795 chemical substances by application Substances 0.000 abstract 5
- 244000063299 Bacillus subtilis Species 0.000 abstract 2
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 241000186660 Lactobacillus Species 0.000 abstract 1
- 241000235342 Saccharomycetes Species 0.000 abstract 1
- 238000010564 aerobic fermentation Methods 0.000 abstract 1
- 244000144974 aquaculture Species 0.000 abstract 1
- 229940039696 lactobacillus Drugs 0.000 abstract 1
- 230000003211 malignant effect Effects 0.000 abstract 1
- 125000000101 thioether group Chemical group 0.000 abstract 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 5
- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 230000000452 restraining effect Effects 0.000 description 5
- 239000003337 fertilizer Substances 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a complex microbial agent and a production method thereof. The technical scheme disclosed by the invention comprises the steps of orderly inoculating the same volume of bacillus subtilis bacteria liquid, yeast solution, and lactobacillus bacterial liquid into a fermentation tank, firstly, carrying out aerobic fermentation cultivation, and then carrying out anaerobic fermentation cultivation, so as to obtain the complex microbial agent, wherein the single bacteria number of bacillus subtilis, saccharomycetes and lactic acid bacteria is 106-107 per mL in the complex microbial agent; and the total bacteria number is 107-108 per mL. The cheap and excellent complex microbial agent is provided by the invention; and the cheap and excellent complex microbial agent can be applied to the fields of fast fermentation of farmyard manure, restraint of propagation of malignant bacteria in soil, decomposition of sulfide in aquaculture water and the like, is simple in production method and easy to operate, and can achieve commercialized popularization.
Description
Technical field
The present invention relates to the complex micro organism fungicide field, refer to especially a kind of a kind of complex micro organism fungicide and production method thereof that can be used for the quick fermentation farm manure.
Background technology
Along with the development of agriculture production and a large amount of uses of chemical fertilizer, soil physical chemistry degradation, environmental pollution phenomenon increase the weight of.Farm manure is with its green, environmental protection, nuisanceless being widely used, but the farm manure complicated component, decompose slow, fertilizer efficiency late, be difficult for becoming thoroughly decomposed, in order to overcome these drawbacks, scientist makes great efforts to explore the decomposition rate that improves farm manure always.
Summary of the invention
The present invention proposes a kind of complex micro organism fungicide and production method thereof, decompose problem slow, that be difficult for becoming thoroughly decomposed in order to solve farm manure.
A kind of complex micro organism fungicide, active ingredient comprises: subtilis, yeast and milk-acid bacteria; The single bacterial classification quantity 10 of subtilis, yeast and milk-acid bacteria
6～10
7Individual/mL, total count amount 10
7～10
8Individual/mL.
The invention allows for a kind of method of producing complex micro organism fungicide, produce the 100L complex micro organism fungicide, comprise following production stage:
Step 1: fermented pretreatment comprises equipment sterilization, medium sterilization and liquid medium preparation:
Equipment sterilization: with fermentor tank and 100～110 ℃ of sterilization 30min～60min of culture medium vessel high temperature, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 ℃;
Medium sterilization: substratum comprises two kinds of low sugar culture-medium and high glucose mediums, will hang down sugar culture-medium and high glucose medium and put into the pressure kettle 1h～3h that sterilizes;
The preparation liquid medium: the low sugar culture-medium after sterilizing and high glucose medium mix with the 1L sterilized water respectively, put into culture medium vessel, stir 30min standby;
Step 2: fermentation culture comprises the cultivation of aerobic cultivation and anaerobism:
To fermentor tank, subtilis bacterium liquid, yeast bacterium liquid and the lactic acid bacterial liquid that will cultivate afterwards the good equal volume of activation are inoculated in fermentor tank in order successively with the low sugar liquid medium drainage that configures, and the volume of three kinds of bacterium liquid is 4～7L; Open the filter air pump, oxygen was carried out aerobic cultivation in 3～4 days;
When PH=5～6, close the filter air pump, afterwards with the high glucose medium liquid drainage to fermentor tank, equipment self-circulation rly. is transferred to carries out anaerobism 12h/ time and cultivated 3～4 days;
Step 3: fermentation aftertreatment: when PH=3～4, turn off the self-circulation rly..
As a kind of preferred technical scheme, low sugar culture-medium comprises the component of following weight: brown sugar 0.2～0.3g, peptone 0.06～0.12g, potassium primary phosphate 0.1～0.3g, sal epsom 0.01～0.03g, Repone K 0.03～0.05g, sodium-chlor 0.03～0.05g, yeast extract 0.3～0.5g, halogen sheet 0.01～0.02g, W-Gum 0.1～0.3g.
As a kind of preferred technical scheme, high glucose medium comprises the component of following weight: glucose 0.2～0.5g, brown sugar 0.4～0.8g, molasses 0.1～0.3g, yeast extract 0.2～0.5g, urea 0.01～0.03g, vitamins C 0.01～0.03g.
As a kind of preferred technical scheme, in subtilis bacterium liquid, the weight ratio of subtilis is 30～40%, and in yeast bacterium liquid, saccharomycetic weight ratio is 20～30%, and in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30～40%.
As to improvement of the technical scheme, in subtilis bacterium liquid, the weight ratio of subtilis is 30%, and in described yeast bacterium liquid, saccharomycetic weight ratio is 30%, and in described lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30%.
The invention provides a kind of complex micro organism fungicide inexpensive, of fine quality, can be used for quick fermentation farm manure, the breeding of restraining the soil unwanted bacteria, the fields such as decomposition of used for aquiculture Sulfide in water, production method is easy, easy to operate, can realize the commercialization popularization.
Description of drawings
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, the below will do to introduce simply to the accompanying drawing of required use in embodiment or description of the Prior Art, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skills, under the prerequisite of not paying creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the Production Flow Chart schematic diagram of a kind of complex micro organism fungicide of proposing of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Based on the embodiment in the present invention, those of ordinary skills belong to the scope of protection of the invention not making the every other embodiment that obtains under the creative work prerequisite.
The present invention proposes a kind of complex micro organism fungicide, active ingredient comprises: subtilis, yeast and milk-acid bacteria; The single bacterial classification quantity 10 of subtilis, yeast and milk-acid bacteria
6～10
7Individual/mL, total count amount 10
7～10
8Individual/mL.
As shown in Figure 1: the invention allows for a kind of method of producing complex micro organism fungicide, produce the 100L complex micro organism fungicide, comprise following production stage:
Step 1: fermented pretreatment comprises equipment sterilization, medium sterilization and liquid medium preparation:
Equipment sterilization: with fermentor tank and 100～110 ℃ of sterilization 30min～60min of culture medium vessel high temperature, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 ℃;
Medium sterilization: substratum comprises two kinds of low sugar culture-medium and high glucose mediums, will hang down sugar culture-medium and high glucose medium and put into the pressure kettle 1h～3h that sterilizes;
The preparation liquid medium: the low sugar culture-medium after sterilizing and high glucose medium mix with the 1L sterilized water respectively, put into culture medium vessel, stir 30min standby;
Step 2: fermentation culture comprises the cultivation of aerobic cultivation and anaerobism:
To fermentor tank, subtilis bacterium liquid, yeast bacterium liquid and the lactic acid bacterial liquid that will cultivate afterwards the good equal volume of activation are inoculated in fermentor tank in order successively with the low sugar liquid medium drainage that configures, and the volume of three kinds of bacterium liquid is 4～7L; Open the filter air pump, oxygen was carried out aerobic cultivation in 3～4 days;
When PH=5～6, close the filter air pump, afterwards with the high glucose medium liquid drainage to fermentor tank, equipment self-circulation rly. is transferred to carries out anaerobism 12h/ time and cultivated 3～4 days;
Step 3: fermentation aftertreatment: when PH=3～4, turn off the self-circulation rly..
Preferably, low sugar culture-medium comprises the component of following weight: brown sugar 0.2～0.3g, peptone 0.06～0.12g, potassium primary phosphate 0.1～0.3g, sal epsom 0.01～0.03g, Repone K 0.03～0.05g, sodium-chlor 0.03～0.05g, yeast extract 0.3～0.5g, halogen sheet 0.01～0.02g, W-Gum 0.1～0.3g.
Preferably, high glucose medium comprises the component of following weight: glucose 0.2～0.5g, brown sugar 0.4～0.8g, molasses 0.1～0.3g, yeast extract 0.2～0.5g, urea 0.01～0.03g, vitamins C 0.01～0.03g.
Preferably, in subtilis bacterium liquid, the weight ratio of subtilis is 30～40%, and in yeast bacterium liquid, saccharomycetic weight ratio is 20～30%, and in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30～40%.
More preferred, in subtilis bacterium liquid, the weight ratio of subtilis is 30%, and in yeast bacterium liquid, saccharomycetic weight ratio is 30%, and in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30%.
The invention provides a kind of complex micro organism fungicide inexpensive, of fine quality, can be used for quick fermentation farm manure, the breeding of restraining the soil unwanted bacteria, the fields such as decomposition of used for aquiculture Sulfide in water, production method is easy, easy to operate, can realize the commercialization popularization.
Embodiment 1:
Step 1: fermented pretreatment comprises equipment sterilization, medium sterilization and liquid medium preparation:
Equipment sterilization: with fermentor tank and 100～110 ℃ of sterilization 30min～60min of culture medium vessel high temperature, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 ℃;
Medium sterilization: described substratum comprises two kinds of low sugar culture-medium and high glucose mediums: low sugar culture-medium comprises the component of following weight: brown sugar 0.2g, peptone 0.06g, potassium primary phosphate 0.1g, sal epsom 0.01g, Repone K 0.03g, sodium-chlor 0.03g, yeast extract 0.3g, halogen sheet 0.01g, W-Gum 0.1g; High glucose medium comprises the component of following weight: glucose 0.2g, brown sugar 0.4g, molasses 0.1g, yeast extract 0.2g, urea 0.01g, vitamins C 0.01g; To hang down sugar culture-medium and high glucose medium and put into pressure kettle sterilization 1h～3h;
The preparation liquid medium: the low sugar culture-medium after sterilizing and high glucose medium mix with the 1L sterilized water respectively, put into culture medium vessel, stir 30min standby;
Step 2: fermentation culture comprises the cultivation of aerobic cultivation and anaerobism:
With the low sugar liquid medium drainage that configures to fermentor tank, subtilis bacterium liquid, yeast bacterium liquid and the lactic acid bacterial liquid that to cultivate afterwards the good equal volume of activation are inoculated in fermentor tank in order successively, wherein: in subtilis bacterium liquid, the weight ratio of subtilis is 30%, in yeast bacterium liquid, saccharomycetic weight ratio is 20%, in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is that the volume of 30%, three kind of bacterium liquid is 4～7L; Open the filter air pump, oxygen was carried out aerobic cultivation in 3～4 days;
When PH=5～6, close the filter air pump, afterwards with the high glucose medium liquid drainage to fermentor tank, equipment self-circulation rly. is transferred to carries out anaerobism 12h/ time and cultivated 3～4 days;
Step 3: fermentation aftertreatment: when PH=3～4, turn off the self-circulation rly..
The invention provides a kind of complex micro organism fungicide inexpensive, of fine quality, can be used for quick fermentation farm manure, the breeding of restraining the soil unwanted bacteria, the fields such as decomposition of used for aquiculture Sulfide in water, production method is easy, easy to operate, can realize the commercialization popularization.
Embodiment 2:
Step 1: fermented pretreatment comprises equipment sterilization, medium sterilization and liquid medium preparation:
Equipment sterilization: with fermentor tank and 100～110 ℃ of sterilization 30min～60min of culture medium vessel high temperature, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 ℃;
Medium sterilization: described substratum comprises two kinds of low sugar culture-medium and high glucose mediums, low sugar culture-medium comprises the component of following weight: brown sugar 0.3g, peptone 0.12g, potassium primary phosphate 0.3g, sal epsom 0.03g, Repone K 0.05g, sodium-chlor 0.05g, yeast extract 0.5g, halogen sheet 0.02g, W-Gum 0.3g; High glucose medium comprises the component of following weight: glucose 0.5g, brown sugar～0.8g, molasses 0.3g, yeast extract 0.5g, urea 0.03g, vitamins C 0.03g.To hang down sugar culture-medium and high glucose medium and put into pressure kettle sterilization 1h～3h;
The preparation liquid medium: the low sugar culture-medium after sterilizing and high glucose medium mix with the 1L sterilized water respectively, put into culture medium vessel, stir 30min standby;
Step 2: fermentation culture comprises the cultivation of aerobic cultivation and anaerobism:
With the low sugar liquid medium drainage that configures to fermentor tank, subtilis bacterium liquid, yeast bacterium liquid and the lactic acid bacterial liquid that to cultivate afterwards the good equal volume of activation are inoculated in fermentor tank in order successively, wherein: in subtilis bacterium liquid, the weight ratio of subtilis is 40%, in yeast bacterium liquid, saccharomycetic weight ratio is 30%, in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is that the volume of 40%, three kind of bacterium liquid is 4～7L; Open the filter air pump, oxygen was carried out aerobic cultivation in 3～4 days;
When PH=5～6, close the filter air pump, afterwards with the high glucose medium liquid drainage to fermentor tank, equipment self-circulation rly. is transferred to carries out anaerobism 12h/ time and cultivated 3～4 days;
Step 3: fermentation aftertreatment: when PH=3～4, turn off the self-circulation rly..
The invention provides a kind of complex micro organism fungicide inexpensive, of fine quality, can be used for quick fermentation farm manure, the breeding of restraining the soil unwanted bacteria, the fields such as decomposition of used for aquiculture Sulfide in water, production method is easy, easy to operate, can realize the commercialization popularization.
Embodiment 3:
Step 1: fermented pretreatment comprises equipment sterilization, medium sterilization and liquid medium preparation:
Equipment sterilization: with fermentor tank and 100～110 ℃ of sterilization 30min～60min of culture medium vessel high temperature, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 ℃;
Medium sterilization: described substratum comprises two kinds of low sugar culture-medium and high glucose mediums, low sugar culture-medium comprises the component of following weight: brown sugar 0.25g, peptone 0.08g, potassium primary phosphate 0.2g, sal epsom 0.02g, Repone K 0.04g, sodium-chlor 0.04g, yeast extract 0.4g, halogen sheet 0.015g, W-Gum 0.2g; High glucose medium comprises the component of following weight: glucose 0.3g, and brown sugar 0.6g, molasses 0.2g, yeast extract 0.3g, urea 0.02g, vitamins C 0.02g will hang down sugar culture-medium and high glucose medium and put into pressure kettle sterilization 1h～3h;
The preparation liquid medium: the low sugar culture-medium after sterilizing and high glucose medium mix with the 1L sterilized water respectively, put into culture medium vessel, stir 30min standby;
Step 2: fermentation culture comprises the cultivation of aerobic cultivation and anaerobism:
With the low sugar liquid medium drainage that configures to fermentor tank, subtilis bacterium liquid, yeast bacterium liquid and the lactic acid bacterial liquid that to cultivate afterwards the good equal volume of activation are inoculated in fermentor tank in order successively, wherein: in subtilis bacterium liquid, the weight ratio of subtilis is 30%, in yeast bacterium liquid, saccharomycetic weight ratio is 30%, and in lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30%;
The volume of three kinds of bacterium liquid is 4～7L; Open the filter air pump, oxygen was carried out aerobic cultivation in 3～4 days;
When PH=5～6, close the filter air pump, afterwards with the high glucose medium liquid drainage to fermentor tank, equipment self-circulation rly. is transferred to carries out anaerobism 12h/ time and cultivated 3～4 days;
Step 3: fermentation aftertreatment: when PH=3～4, turn off the self-circulation rly..
The invention provides a kind of complex micro organism fungicide inexpensive, of fine quality, can be used for quick fermentation farm manure, the breeding of restraining the soil unwanted bacteria, the fields such as decomposition of used for aquiculture Sulfide in water, production method is easy, easy to operate, can realize the commercialization popularization.
These are only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (6)
1. a complex micro organism fungicide, is characterized in that, active ingredient comprises: subtilis, yeast and milk-acid bacteria; The single bacterial classification quantity 10 of described subtilis, described yeast and described milk-acid bacteria
6～10
7Individual/mL, total count amount 10
7～10
8Individual/mL.
2. a method of producing complex micro organism fungicide as claimed in claim 1, is characterized in that, produces the described complex micro organism fungicide of 100L, comprises following production stage:
Step 1: fermented pretreatment comprises equipment sterilization, medium sterilization and liquid medium preparation:
Equipment sterilization: with fermentor tank and 100～110 ℃ of sterilization 30min～60min of culture medium vessel, add afterwards the boiling water after the filtration of container volume 80%, and be cooled to 30～45 ℃;
Medium sterilization: described substratum comprises two kinds of low sugar culture-medium and high glucose mediums, will described low sugar culture-medium and described high glucose medium put into the pressure kettle 1h～3h that sterilizes;
The preparation liquid medium: the described low sugar culture-medium after sterilizing and described high glucose medium mix with the 1L sterilized water respectively, put into described culture medium vessel, stir 30min standby;
Step 2: fermentation culture comprises the cultivation of aerobic cultivation and anaerobism:
With the described low sugar liquid medium drainage that configures to described fermentor tank, described subtilis bacterium liquid, described yeast bacterium liquid and the described lactic acid bacterial liquid that to cultivate afterwards the good equal volume of activation are inoculated in described fermentor tank in order successively, and the volume of three kinds of bacterium liquid is 4～7L; Open the filter air pump, oxygen was carried out aerobic cultivation in 3～4 days;
When PH=5～6, close described filter air pump, afterwards with described high glucose medium liquid drainage to described fermentor tank, equipment self-circulation rly. is transferred to 12h/ time carries out anaerobism cultivation 3～4 days;
Step 3: fermentation aftertreatment: when PH=3～4, turn off described self-circulation rly..
3. a kind of method of producing complex micro organism fungicide as claimed in claim 2, it is characterized in that: described low sugar culture-medium comprises the component of following weight: brown sugar 0.2～0.3g, peptone 0.06～0.12g, potassium primary phosphate 0.1～0.3g, sal epsom 0.01～0.03g, Repone K 0.03～0.05g, sodium-chlor 0.03～0.05g, yeast extract 0.3～0.5g, halogen sheet 0.01～0.02g, W-Gum 0.1～0.3g.
4. a kind of method of producing complex micro organism fungicide as claimed in claim 2, it is characterized in that, described high glucose medium comprises the component of following weight part: glucose 0.2～0.5g, brown sugar 0.4～0.8g, molasses 0.1～0.3g, yeast extract 0.2～0.5g, urea 0.01～0.03g, vitamins C 0.01～0.03g.
5. a kind of method of producing complex micro organism fungicide as claimed in claim 2, it is characterized in that: in described subtilis bacterium liquid, the weight ratio of subtilis is 30～40%, in described yeast bacterium liquid, saccharomycetic weight ratio is 20～30%, and in described lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30～40%.
6. a kind of method of producing complex micro organism fungicide as claimed in claim 5, it is characterized in that, in described subtilis bacterium liquid, the weight ratio of subtilis is 30%, in described yeast bacterium liquid, saccharomycetic weight ratio is 30%, and in described lactic acid bacterial liquid, the weight ratio of milk-acid bacteria is 30%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310125401.5A CN103173399B (en) | 2013-04-11 | 2013-04-11 | Complex microbial agent and production method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310125401.5A CN103173399B (en) | 2013-04-11 | 2013-04-11 | Complex microbial agent and production method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103173399A true CN103173399A (en) | 2013-06-26 |
| CN103173399B CN103173399B (en) | 2014-12-03 |
Family
ID=48633593
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310125401.5A Expired - Fee Related CN103173399B (en) | 2013-04-11 | 2013-04-11 | Complex microbial agent and production method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103173399B (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104726103A (en) * | 2015-03-31 | 2015-06-24 | 山东省林业科学研究院 | Microorganism combined bacterium solution for drought mountain land and gel preparation of microorganism combined bacterium solution |
| CN104788262A (en) * | 2015-04-10 | 2015-07-22 | 张永松 | Organic liquid fertilizer for C3 type crops, and preparation method and application method thereof |
| CN104862247A (en) * | 2015-04-30 | 2015-08-26 | 山西师范大学 | Liquid medium of composite microbial agent and preparation method thereof |
| CN105274016A (en) * | 2014-06-09 | 2016-01-27 | 济源市国龙生物科技有限公司 | Microbial straw fermentation agent and method for producing straw forage by utilizing fermentation agent |
| CN105567604A (en) * | 2016-02-16 | 2016-05-11 | 江苏中通生物科技有限公司 | Compound microorganism bacterium agent and preparation method thereof |
| CN106348817A (en) * | 2016-08-26 | 2017-01-25 | 山东省鲁洲食品集团有限公司 | Process for preparing liquid bio-fertilizer by using corn starch sugar wastes |
| CN107259074A (en) * | 2017-08-11 | 2017-10-20 | 广西农垦西江乳业有限公司奶牛场 | A kind of solid fungicide and its preparation and application for being used to make silage corn |
| CN109943491A (en) * | 2019-04-23 | 2019-06-28 | 漳州旺德生物科技有限公司 | A kind of method for extracting and culturing of composite microbial fermentation bacteria |
| CN111728223A (en) * | 2020-07-07 | 2020-10-02 | 西安因子元素科技有限公司 | Method for producing compound cytokine elements by fermentation |
| CN111978120A (en) * | 2020-08-25 | 2020-11-24 | 湖南启农生物科技有限公司 | Liquid environment-friendly composite microbial fertilizer and preparation method thereof |
| CN113308324A (en) * | 2021-06-01 | 2021-08-27 | 厦门元之道生物科技有限公司 | Preparation method of plant-based low-sugar beverage fermented by natural yeast |
| CN114276186A (en) * | 2021-12-17 | 2022-04-05 | 广东恒懋生物农业科技有限公司 | Efficient biological bacterial fertilizer and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186879A (en) * | 2007-12-05 | 2008-05-28 | 中国科学院南京土壤研究所 | Agricultural waste composting ternary microbial compound bacterial agent |
| CN101724559A (en) * | 2009-12-08 | 2010-06-09 | 营口恒新生物技术开发有限公司 | Compound microbial inoculum for decomposing straw as well as preparation method and application thereof |
| CN101748064A (en) * | 2008-12-15 | 2010-06-23 | 郑州环球生物环保技术有限公司 | Highly-efficient living microbe composite preparation |
-
2013
- 2013-04-11 CN CN201310125401.5A patent/CN103173399B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186879A (en) * | 2007-12-05 | 2008-05-28 | 中国科学院南京土壤研究所 | Agricultural waste composting ternary microbial compound bacterial agent |
| CN101748064A (en) * | 2008-12-15 | 2010-06-23 | 郑州环球生物环保技术有限公司 | Highly-efficient living microbe composite preparation |
| CN101724559A (en) * | 2009-12-08 | 2010-06-09 | 营口恒新生物技术开发有限公司 | Compound microbial inoculum for decomposing straw as well as preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 周可等: "不同微生物菌剂处理对鸡粪堆肥发酵的影响", 《天津农业科学》, vol. 15, no. 3, 31 December 2009 (2009-12-31) * |
| 贾聪俊等: "接种微生物菌剂对猪粪堆肥效果的影响", 《家畜生态学报》, vol. 32, no. 5, 30 September 2011 (2011-09-30) * |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105274016A (en) * | 2014-06-09 | 2016-01-27 | 济源市国龙生物科技有限公司 | Microbial straw fermentation agent and method for producing straw forage by utilizing fermentation agent |
| CN104726103A (en) * | 2015-03-31 | 2015-06-24 | 山东省林业科学研究院 | Microorganism combined bacterium solution for drought mountain land and gel preparation of microorganism combined bacterium solution |
| CN104788262A (en) * | 2015-04-10 | 2015-07-22 | 张永松 | Organic liquid fertilizer for C3 type crops, and preparation method and application method thereof |
| CN104862247A (en) * | 2015-04-30 | 2015-08-26 | 山西师范大学 | Liquid medium of composite microbial agent and preparation method thereof |
| CN105567604A (en) * | 2016-02-16 | 2016-05-11 | 江苏中通生物科技有限公司 | Compound microorganism bacterium agent and preparation method thereof |
| CN106348817A (en) * | 2016-08-26 | 2017-01-25 | 山东省鲁洲食品集团有限公司 | Process for preparing liquid bio-fertilizer by using corn starch sugar wastes |
| CN107259074A (en) * | 2017-08-11 | 2017-10-20 | 广西农垦西江乳业有限公司奶牛场 | A kind of solid fungicide and its preparation and application for being used to make silage corn |
| CN109943491A (en) * | 2019-04-23 | 2019-06-28 | 漳州旺德生物科技有限公司 | A kind of method for extracting and culturing of composite microbial fermentation bacteria |
| CN111728223A (en) * | 2020-07-07 | 2020-10-02 | 西安因子元素科技有限公司 | Method for producing compound cytokine elements by fermentation |
| CN111978120A (en) * | 2020-08-25 | 2020-11-24 | 湖南启农生物科技有限公司 | Liquid environment-friendly composite microbial fertilizer and preparation method thereof |
| CN113308324A (en) * | 2021-06-01 | 2021-08-27 | 厦门元之道生物科技有限公司 | Preparation method of plant-based low-sugar beverage fermented by natural yeast |
| CN113308324B (en) * | 2021-06-01 | 2023-07-25 | 厦门元之道生物科技有限公司 | Preparation method of plant-based low-sugar beverage fermented by natural yeast |
| CN114276186A (en) * | 2021-12-17 | 2022-04-05 | 广东恒懋生物农业科技有限公司 | Efficient biological bacterial fertilizer and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103173399B (en) | 2014-12-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103173399B (en) | Complex microbial agent and production method thereof | |
| CN103980017B (en) | The method of the quick production high quality organic fertilizer of high-temperature aerobic Secondary Fermentation | |
| CN102051335A (en) | Method for producing microbial ecological agent by using kitchen garbage | |
| CN103305434A (en) | Microecological preparation with dual functions of probiotics and organic selenium and preparation method of microecological preparation | |
| CN104388348A (en) | Microbial preparation for purifying sewage and deodorizing garbage and preparation method thereof | |
| CN104388350A (en) | Microbial agent capable of improving quality of water for aquaculture and method for preparing same | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN105777234A (en) | Method for preparing biological organic fertilizer through common fermentation of nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium bacteria | |
| CN103710286A (en) | Processing method of viable clostridium butyricum preparation and culture medium composition | |
| CN101914445A (en) | Indigenous probiotic microorganism solid fungicide and preparation method and application thereof | |
| CN101857469B (en) | Preparation process for golf course lawn slow-release bio-organic fertilizer produced by three continuous fermentations | |
| CN103881929A (en) | Purification compound bacterium and purification compound probiotic preparation as well as preparation method of compound probiotic preparation | |
| CN101407761B (en) | Liquid inocula composed of yeast fused strain, Geotrichum candidum Link and Rhizopus, and preparation and use thereof | |
| CN103992977A (en) | Method for culturing bacillus subtilis through high-density fermentation | |
| CN101659570A (en) | Preparation method of biofertilizer | |
| CN102943058A (en) | Method for anaerobic shake culture of photosynthetic bacteria by utilizing biogas slurry | |
| CN105861367A (en) | Pig manure methane fermenting agent formula and preparation method thereof | |
| CN103289911A (en) | Solid fermentation method for bacillus subtilis | |
| CN103289912A (en) | Solid fermentation method of bacillus coagulans | |
| CN103614286B (en) | Method and device for cultivating high-concentration photosynthetic bacteria with low illumination | |
| CN103255126A (en) | Method for preparing solid EM | |
| CN211005347U (en) | Culture bag for culturing aquatic lactic acid bacteria | |
| CN108395316A (en) | A kind of carbon-based bio-bacterial manure and preparation method thereof | |
| CN218666019U (en) | A breeding device for microorganism immobilization | |
| CN102943043A (en) | High-efficiency agricultural microecological compound bacterium agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141203 Termination date: 20160411 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |