CN103170382A - Machining process of biochip microfluidic module - Google Patents
Machining process of biochip microfluidic module Download PDFInfo
- Publication number
- CN103170382A CN103170382A CN2013100395622A CN201310039562A CN103170382A CN 103170382 A CN103170382 A CN 103170382A CN 2013100395622 A CN2013100395622 A CN 2013100395622A CN 201310039562 A CN201310039562 A CN 201310039562A CN 103170382 A CN103170382 A CN 103170382A
- Authority
- CN
- China
- Prior art keywords
- micro
- utilize
- numerically
- biochip
- shape
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to a machining process of a PMMA (Polymethyl methacrylate) microfluidic module for washing and crossing a biochip. The machining process comprises the following steps of: firstly, machine the surface of a square PMMA block material according to the size of a sealing rubber cushion by utilizing a precise micro-milling technology; then, vertically machining liquid path micro-pores along the direction of a fitting face of the biochip according to an array position of the biochip; then machining a liquid inlet micro-pore and a liquid outlet micro-pore at the two sides by utilizing a numerical control precise long-range drilling technology; and respectively communicating the liquid inlet micro-pore and the liquid outlet micro-pore with the liquid path micro-pores which are vertically machined to form a liquid outlet channel, so as to finish the manufacturing of the PMMA microfluidic module. According to the machining process disclosed by the invention, complicated instruments and strict experiment conditions are not needed and diversified biochip microfluidic templates can be manufactured; and the machining process disclosed by the invention has the characteristics of no pollution, integrity in molding, simple preparation process, low cost, short production period, high micro-channel transparency, capability of being produced in batches and the like, and has a wide application prospect.
Description
Technical field
The present invention relates to biological chip miniflow road module processing technology, utilize the processing of modern precision Computerized Numerical Control processing technology monolithic molding, biochip cleans, microfluidic circuit is used in hybridization, belongs to the processing and fabricating field.
Background technology
The microfluidic circuit technology is a frontier interdisciplinary, its target is to utilize the microminiaturization of micro-processing technology Realization analysis chemical device and integrated, and the material that is used for facture of microchip mainly comprises: glass, quartz, dimethyl silicone polymer (PDMS), polymethyl methacrylate (PMMA) etc.Wherein, glass and quartz are the micro-fluidic chip base materials that is most widely used, processing method mainly contains following three kinds, first method is to use the sealing-in of optical cement method, the Major Difficulties of this technology is to exist larger sealing-in area, two surface of glass slide are difficult to fit like a glove, and often accompany portion gas between slide, thereby hinder the sealing-in of slide.The method needs slide is carried out strict polishing, and process is complicated, takes time and effort, and cost is high, and yield rate is low.The second is the optical etching technology that adopts extensive use on microelectronics industry.Yet the photoengraving process is complicated, consuming time, needs expensive special equipment and clean facility, and material brittle, can not satisfy the needs increasing to micro element at present.The third is the glass bonding techniques, this specification requirement bonding surface has very high flatness and surperficial compatibility, require stricter surface clean, this makes the chip manufacturing process complicated, even utilize HF acid, realized bonding under room temperature, needed bonding conditions is still stricter, need to carry out at Clean room, exist the adhesive layer bonding to change the inner-walls of duct pattern, and line clogging easily occurs, and bonding needs strict HF acid concentration and pressure, and HF acid has the strong problems such as corrosiveness to glass.Therefore, should further explore inexpensive durable material and fast and convenient fluid channel manufacturing technology is beneficial to the universal of microfluidic system and develops.The present invention does not need complicated instrument and strict experiment condition, just can make diversified biochip and clean the hybridization template, compare with the template of utilizing the acquisitions such as above technique, have easy processing, preparation technology is simple, cost is low, with short production cycle, microchannel Bao Tongdugao, the characteristics such as can manufacture.And the template of making has good stability, can reuse, and has greatly improved the service life of template.
Summary of the invention
Purpose of the present invention is intended to overcome the deficiencies in the prior art and bases oneself upon the automation mechanized operation process module that modern high-precision numerical control process technology designs a kind of biochip hybridization and cleaning process, improves biochip in the application level of the aspects such as Polymorphism Analysis and medical diagnosis on disease.
the technical scheme that the present invention solves the problems of the technologies described above is as follows: utilize numerically-controlled precise long-range Drill Techniques to shape the horizontal pipeline of fluid miniflow and vertical pipeline in module one side, utilize the numerically-controlled precise milling technology to shape fluid screw thread button hole, opposite side in module is of a size of benchmark with the genetic chip array element, be basic point along the diagonal summit, utilize numerically-controlled precise long-range Drill Techniques to shape the horizontal pipeline of feed liquor miniflow and vertical pipeline, utilize the numerically-controlled precise milling technology to shape feed liquor screw thread button hole, at last take biochip array element size and sealing rubber pad appearance and size as benchmark, utilize the numerically-controlled precise milling technology to shape the sealing deep gouge.
The invention has the beneficial effects as follows:
1, simple for structure reliable, assembling and setting is convenient, and the biochip hybridization system good stability reduces related personnel's Professional Demand;
2, the biological chip miniflow road module processing technology of simple for structure, simple to operate, reasonable price, dependable performance has realistic meaning to the penetration and promotion of biochip.
On the basis of technique scheme, the present invention can also do following improvement.
Further, described module whole milling is shaped, and thickness is the 8-9 millimeter, and matrix is the PMMA material.
Adopt the beneficial effect of above-mentioned further scheme to be, be conducive to guarantee uniformity that all modules shape and high yield rate.
Further, described utilize the numerically-controlled precise milling technology to shape fluid screw thread button hole and feed liquor screw thread button hole after, button hole bottom milling escape.
Adopt the beneficial effect of above-mentioned further scheme to be, guarantee the reliability of sealing effectiveness, the dismounting of being convenient to the stream accessory is cleaned.
Further, after described module whole shapes, under room temperature environment, take out after 24 hours with immersion in 95% alcoholic solution, the alcoholic solution of use 95% cleans its duct, dries up at last.
Adopt the beneficial effect of above-mentioned further scheme to be, guarantee the pure of stream, avoid artificial processing pollution.
Description of drawings
Fig. 1 is the structural representation that the present invention is used for biological chip miniflow road module processing technology;
Fig. 2 is the A-A view of Fig. 1;
Fig. 3 is the B-B view of Fig. 1;
Fig. 4 is the C-C view of Fig. 1;
Fig. 5 is the axonometric drawing of Fig. 1;
In accompanying drawing, the list of parts of each label representative is as follows:
1, fluid screw thread button hole, 2, the horizontal pipeline of fluid miniflow, 3, the fluid vertical pipeline, 4, the sealing deep gouge, 5, feed liquor screw thread button hole, 6, the horizontal pipeline of feed liquor miniflow, 7, the feed liquor vertical pipeline
The specific embodiment
Below in conjunction with accompanying drawing, principle of the present invention and feature are described, example only is used for explaining the present invention, is not be used to limiting scope of the present invention.
biological chip miniflow road module processing technology is to utilize numerically-controlled precise long-range Drill Techniques to shape the horizontal pipeline 2 of fluid miniflow and fluid vertical pipeline 3 in module one side, utilize the numerically-controlled precise milling technology to shape fluid screw thread button hole 1, opposite side in module is of a size of benchmark with the genetic chip array element, be basic point along the diagonal summit, utilize numerically-controlled precise long-range Drill Techniques to shape the horizontal pipeline 6 of feed liquor miniflow and feed liquor vertical pipeline 7, utilize the numerically-controlled precise milling technology to shape feed liquor screw thread button hole 5, at last take biochip array element size and sealing rubber pad appearance and size as benchmark, utilize the numerically-controlled precise milling technology to shape sealing deep gouge 4.
Described module whole milling is shaped, and thickness is the 8-9 millimeter, and matrix is the PMMA material.
Described utilize the numerically-controlled precise milling technology to shape fluid screw thread button hole (1) and feed liquor screw thread button hole 5 after, button hole bottom milling escape.
After described module whole shapes, under room temperature environment, take out after 24 hours with immersion in 95% alcoholic solution, the alcoholic solution of use 95% cleans its duct, dries up at last.
The operation principle that the present invention is used for biological chip miniflow road module processing technology is as follows:
At first utilize accurate little milling technology with square PMMA piece material surface by the sealing rubber pad dimensioned out, again according to the biochip array position along with biochip binding face perpendicular direction working fluid road micropore, then utilize numerically-controlled precise long-range Drill Techniques to process feed liquor micropore and fluid micropore in both sides, its respectively with vertical working fluid road micropore together with, construct fluid passage, complete the making of PMMA microfluidic circuit module.
The above is only preferred embodiment of the present invention, and is in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, is equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (4)
1. biological chip miniflow road module processing technology, it is characterized in that: utilize numerically-controlled precise long-range Drill Techniques to shape the horizontal pipeline of fluid miniflow (2) and fluid vertical pipeline (3) in module one side, utilize the numerically-controlled precise milling technology to shape fluid screw thread button hole (1), opposite side in module is of a size of benchmark with the biochip array element, be basic point along the diagonal summit, utilize numerically-controlled precise long-range Drill Techniques to shape the horizontal pipeline of feed liquor miniflow (6) and feed liquor vertical pipeline (7), utilize the numerically-controlled precise milling technology to shape feed liquor screw thread button hole (5), at last take biochip array element size and sealing rubber pad appearance and size as benchmark, utilize the numerically-controlled precise milling technology to shape sealing deep gouge (4).
2. biological chip miniflow according to claim 1 road module processing technology, it is characterized in that: described module whole milling is shaped, and thickness is the 8-9 millimeter, and matrix is the PMMA material.
3. biological chip miniflow according to claim 1 road module processing technology is characterized in that: described utilize the numerically-controlled precise milling technology to shape fluid screw thread button hole (1) and feed liquor screw thread button hole (5) after, button hole bottom milling escape.
4. biological chip miniflow according to claim 1 road module processing technology, it is characterized in that: after described module whole shapes, under room temperature environment, take out after 24 hours with immersion in 95% alcoholic solution, the alcoholic solution of use 95% cleans its duct, dries up at last.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100395622A CN103170382A (en) | 2013-02-01 | 2013-02-01 | Machining process of biochip microfluidic module |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100395622A CN103170382A (en) | 2013-02-01 | 2013-02-01 | Machining process of biochip microfluidic module |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103170382A true CN103170382A (en) | 2013-06-26 |
Family
ID=48630825
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100395622A Pending CN103170382A (en) | 2013-02-01 | 2013-02-01 | Machining process of biochip microfluidic module |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103170382A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110008211A1 (en) * | 2007-10-12 | 2011-01-13 | Hunor Santha | Microfluid channel, method for its implementation, and microfluidic system containing said channel |
| CN102442633A (en) * | 2010-10-14 | 2012-05-09 | 北京华凯瑞微流控芯片科技有限责任公司 | Numerically controlled processing instrument for microfluidic chip |
-
2013
- 2013-02-01 CN CN2013100395622A patent/CN103170382A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110008211A1 (en) * | 2007-10-12 | 2011-01-13 | Hunor Santha | Microfluid channel, method for its implementation, and microfluidic system containing said channel |
| CN102442633A (en) * | 2010-10-14 | 2012-05-09 | 北京华凯瑞微流控芯片科技有限责任公司 | Numerically controlled processing instrument for microfluidic chip |
Non-Patent Citations (4)
| Title |
|---|
| 中国科学技术协会主编: "《2008-2009机械工程学科发展报告(机械制造)》", 31 March 2009 * |
| 刘战强等: "微切削加工技术", 《工具技术》 * |
| 包杰等: "基于PC的开放式数控系统微铣削伺服控制的研究", 《机械科学与技术》 * |
| 王续跃等: "模具钢微流道沟槽的激光铣削试验研究", 《兵工学报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103038331B (en) | Reagent fluid dispensing device, and method of dispensing a reagent fluid | |
| CN107305214B (en) | A kind of production method of hard micro-fluid chip | |
| CN105296349A (en) | Microfluidic chip, detection system and device used for rapid DNA detection | |
| CN102580799B (en) | Machining method of micro-drop and micro-fluidic control chip | |
| CN103389303A (en) | Multi-index analysis paper chip and preparation method thereof | |
| CN107192475B (en) | Temperature sensor system based on micro-fluidic chip and preparation method | |
| WO2008147428A1 (en) | Systems and methods for active microfluidic cell handling | |
| CN101533005A (en) | Microflow distribution device, manufacturing method and application thereof | |
| CN205170857U (en) | A micro -fluidic chip , detecting system and device for DNA short -term test | |
| CN101505874A (en) | A method and apparatus for attaching a fluid cell to a planar substrate | |
| CN205127987U (en) | Micro -fluidic chip for multi -index detection | |
| CN103723676A (en) | Manufacturing method of micro-fluid channel | |
| CN105772125A (en) | 3D printing-based microfluidic chip fixture experiment platform | |
| CN103357453A (en) | Microfluidic chip and machining process thereof | |
| CN103263950A (en) | Manufacture method of glass base heterozygosis micro-fluidic chip | |
| CN108212231A (en) | A kind of miniflow macrofluid control chip and preparation method thereof | |
| CHANG et al. | Progress of microfluidics based on printed circuit board and its applications | |
| Pfreundt et al. | An easy-to-use microfluidic interconnection system to create quick and reversibly interfaced simple microfluidic devices | |
| CN103170382A (en) | Machining process of biochip microfluidic module | |
| CN108837718A (en) | A kind of high throughput microlayer model gradient dilution device and method | |
| CN102896009B (en) | Free flow electrophoresis chip of adhesive tape and processing method thereof | |
| CN109746060A (en) | Microlayer model generates chip | |
| CN209952893U (en) | Micro-fluidic paster | |
| CN106280465A (en) | A kind of preparation method of polydimethylsiloxane porous membrane | |
| CN103834559B (en) | Micro-fluidic chip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130626 |