CN105772125A - 3D printing-based microfluidic chip fixture experiment platform - Google Patents
3D printing-based microfluidic chip fixture experiment platform Download PDFInfo
- Publication number
- CN105772125A CN105772125A CN201610258731.5A CN201610258731A CN105772125A CN 105772125 A CN105772125 A CN 105772125A CN 201610258731 A CN201610258731 A CN 201610258731A CN 105772125 A CN105772125 A CN 105772125A
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- Prior art keywords
- cover plate
- micro
- fluidic chip
- substrate
- rectangular
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
Abstract
The invention discloses a 3D printing-based microfluidic chip fixture experiment platform. A base plate and a cover plate are two main structures of an experiment platform and are connected through a bolt, wherein rectangular windows of the same size, four bolt through holes and four rectangular bulge positioning settings are respectively reserved in the centers of the base plate and the cover plate; a slope type microfluidic chip insertion port is formed in the base plate, and a left positioning clamp station and a right positioning clamp station are arranged in the base plate; six connecting tube insertion ports and a seal ring mounting position are respectively distributed on each of the left side and the right side of the cover plate. The rectangular windows of the same size, the four bolt through holes and the four rectangular bulge positioning settings are respectively reserved in the centers of the base plate and the cover plate; the slope type microfluidic chip insertion port is formed in the base plate, and the left positioning clamp station and the right positioning clamp station are arranged in the base plate; six connecting tube insertion ports and the seal ring mounting position are respectively distributed on each of the left side and the right side of the cover plate, so that the 3D printing-based microfluidic chip fixture experiment platform is suitable for experimental detection of all types of microfluidic chips and the laboratory use, and can achieve the purposes of rapid processing, low manufacturing cost, simple and convenient operation, and effect improvement of experimental connecting tubes.
Description
Technical field
The present invention relates to a kind of based on the 3D micro-fluidic chip clamp experiment porch printed, specifically, it is that 3D printing technique is applied in the making of micro-fluidic experiment porch, realize the rapid processing of experiment porch, reduce cost of manufacture, purpose easy and simple to handle, this fixture experiment porch is applicable to biomedical detection and the chemical analysis application of various employing micro-fluidic chips, belongs to microfluidic art.
Background technology
3D prints the one belonging to rapid shaping technique, and its central principle is: " Layered manufacturing, successively superposition ".The printed material of 3D printer is the raw material of device, such as metal, pottery, plastics, sand etc..The process of implementing is to utilize computer-aided design or modeling software to commodity modeling, then model division is become cross section successively, generates STL standard data file.3D printer, by identifying file middle section information, successively prints and bonding, produces entity.It is widely used in the fields such as automobile, aviation, medical treatment, building at present.Its principle increasing material manufacture is greatly saved material, reduces product cost, constant product quality.But for the product that required precision is significantly high, it is necessary to follow-up polishing and polish；Because in the course of processing, material is heated repeatedly, therefore interiors of products stress is complicated, product material poor-performing, for instance intensity, rigidity etc..So, it is highly advantageous that 3D prints product not high for required precision, that product strength requirement is not high yet.
Microfluidic chip technology is one of focus of present analysis science research.Micro-fluidic chip is that the basic operation units such as biology, chemistry, the sample preparation of medical analysis process, reaction, separation, detection are integrated on the chip of one piece of micro-meter scale, is automatically performed analysis overall process.Microfluidic chip analysis is with chip for operating platform, simultaneously based on analytical chemistry, with micro electronmechanical process technology for relying on, with microchannel network for architectural feature, with life sciences for currently mainly application, is the emphasis of current micro-total analysis system field development.And laboratory is when carrying out micro-fluidic chip experiment, it is necessary to by connecting tube, fluid is imported in micro-fluidic chip, therefore experiment is required for connecting connecting tube at each interface every time, is sealed simultaneously.Each micro-fluidic chip has some interfaces, and excuse size is little, so adapter operation can consume the plenty of time when experiment, and quality of connection is relevant to manual operation stability, seepage and blocking easily occurs.The unreasonable arrangement of connecting tube also can affect laboratory observation and result.
Summary of the invention
In order to overcome micro-fluidic chip connecting tube in existing laboratory work to rely on the problem that experimenter's operating experience, length consuming time and connection procedure are loaded down with trivial details, the present invention provides a kind of based on the 3D micro-fluidic chip clamp experiment porch printed, experimenter should be not only assisted in based on the 3D micro-fluidic chip clamp experiment porch printed and quickly connect the connecting tube of turnover micro-fluidic chip, and can guarantee that quality of connection, operation is simple.
The technical solution adopted for the present invention to solve the technical problems is: of the present invention based on the 3D micro-fluidic chip clamp experiment porch printed, including substrate, cover plate, bolt connecting hole, substrate and cover plate two parts are experiment porch agent structure, two parts are bolted, substrate and cover plate central leave the rectangular window of formed objects, and four bolt holes, four rectangular preiection location are arranged, substrate has ramp type micro-fluidic chip insert port, there is location, left and right chuck inside, cover plate is each side distributed six connecting tube insert ports and sealing ring installation site.
Described substrate and cover plate print by 3D and make.
It is all provided with six about described connecting tube insert port, arranges according to rectangular mode.
Described substrate has ramp type micro-fluidic chip insert port.
Substrate and cover plate have multiple positioning modes, connect including rectangular preiection, location, left and right chuck, bolt.
Apparatus of the present invention design is succinct, available 3D prints, realize low cost rapid processing, ramp type micro-fluidic chip insert port makes micro-fluidic chip to insert smoothly, it is damaged from, rectangular preiection, location, left and right chuck, bolt connection make substrate and cover plate be accurately positioned, and multiple connecting tube insert ports can meet the design requirement of different micro-fluidic chips in subsequent experimental.
The invention has the beneficial effects as follows, it is possible to save the loaded down with trivial details fluid that connects and flow into the process of pipe, it is only necessary to micro-fluidic chip is inserted substrate cover upper cover plate and tightens fixing bolt, convenient succinct, it is ensured that the quality of connection of connecting tube.
Accompanying drawing explanation
Fig. 1 is that the substrate cover of the present invention hardens composition.
In figure: 1, bolt connecting hole, 2, connecting tube insert port, 3, sealing ring, 4, cover plate, 5, substrate, 6, rectangular window, 7, rectangular preiection, 8, location, left and right chuck, 9, ramp type micro-fluidic chip insert port, 10, rectangular recess.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is further described.
In FIG, utilize structure designed by 3D printing and making, cover plate (4) is provided with sealing ring (3), installation site installs the not easily sealing ring of flow-induced corrosion material used by experiment, connecting tube is installed in connecting tube insert port (2) position, substrate (5) is directed at cover plate (4), and rectangular preiection (7) is directed at rectangular recess (10)；After bolt is connected with bolt connecting hole (1), substrate (5) and cover plate (4) fix；During experiment, micro-fluidic chip is entered from ramp type micro-fluidic chip insert port (9), utilize location, location chuck, left and right (8), rectangular window (6) can observe fluid state in micro-fluidic chip, also can be placed directly within basis of microscopic observation, when terminating experiment, micro-fluidic chip can be ejected from bottom rectangle window (6).
Claims (6)
1. based on the 3D micro-fluidic chip clamp experiment porch printed, it is characterized in that: this platform includes substrate, cover plate, bolt connecting hole, substrate and cover plate two parts are experiment porch agent structure, two parts are bolted, substrate and cover plate central leave the rectangular window of formed objects and four bolt holes, four rectangular preiections location are arranged, substrate has ramp type micro-fluidic chip insert port, inside to have location, left and right chuck, and cover plate is each side distributed six connecting tube insert ports and sealing ring installation site.
2. according to claim 1 based on the 3D micro-fluidic chip clamp experiment porch printed, it is characterised in that: described substrate and cover plate print by 3D and make.
3. according to claim 1 based on the 3D micro-fluidic chip clamp experiment porch printed, it is characterised in that: it is all provided with six about described connecting tube insert port, arranges according to rectangular mode.
4. according to claim 1 based on the 3D micro-fluidic chip clamp experiment porch printed, it is characterised in that: described substrate has ramp type micro-fluidic chip insert port.
5. according to claim 1 based on the 3D micro-fluidic chip clamp experiment porch printed, it is characterised in that: substrate and cover plate have multiple positioning modes, connect including rectangular preiection, location, left and right chuck, bolt.
6. according to claim 1 based on the 3D micro-fluidic chip clamp experiment porch printed, it is characterized in that: cover plate (4) is provided with sealing ring (3), installation site installs the not easily sealing ring of flow-induced corrosion material used by experiment, connecting tube is installed in connecting tube insert port (2) position, substrate (5) is directed at cover plate (4), and rectangular preiection (7) is directed at rectangular recess (10)；After bolt is connected with bolt connecting hole (1), substrate (5) and cover plate (4) fix；During experiment, micro-fluidic chip is entered from ramp type micro-fluidic chip insert port (9), utilize location, location chuck, left and right (8), rectangular window (6) can observe fluid state in micro-fluidic chip, also can be placed directly within basis of microscopic observation, when terminating experiment, micro-fluidic chip can be ejected from bottom rectangle window (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610258731.5A CN105772125B (en) | 2016-04-23 | 2016-04-23 | Micro-fluidic chip clamp experiment porch based on 3D printing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610258731.5A CN105772125B (en) | 2016-04-23 | 2016-04-23 | Micro-fluidic chip clamp experiment porch based on 3D printing |
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| CN105772125A true CN105772125A (en) | 2016-07-20 |
| CN105772125B CN105772125B (en) | 2018-09-21 |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018081920A1 (en) * | 2016-11-01 | 2018-05-11 | 深圳华大智造科技有限公司 | Gene sequencing chip and mounting frame thereof |
| CN108311177A (en) * | 2018-01-15 | 2018-07-24 | 中国科学院上海微系统与信息技术研究所 | Align structures, alignment assemble method and application thereof for 3D PDMS facture of microchip |
| CN110252436A (en) * | 2019-07-03 | 2019-09-20 | 水利部中国科学院水工程生态研究所 | A kind of detachable planktonic organism micro-fluidic chip for high power micro image collection |
| CN110387321A (en) * | 2018-04-23 | 2019-10-29 | 深圳华大智造科技有限公司 | Gene sequencing chip and gene sequencing device |
| CN110564610A (en) * | 2019-10-15 | 2019-12-13 | 杭州比芯诊断技术有限公司 | Double-temperature-zone PCR amplification device |
| CN111356528A (en) * | 2017-11-22 | 2020-06-30 | 惠普发展公司，有限责任合伙企业 | Multi-region microfluidic devices |
| CN112113901A (en) * | 2020-09-18 | 2020-12-22 | 深圳先进技术研究院 | Chip fixing device |
| CN112673090A (en) * | 2018-10-29 | 2021-04-16 | Nok株式会社 | Micro-fluidic chip and micro-fluidic device |
| CN113059511A (en) * | 2021-03-19 | 2021-07-02 | 杭州比芯诊断技术有限公司 | Microfluidic PCR plate fixing device |
| CN118079818A (en) * | 2024-03-06 | 2024-05-28 | 暨南大学 | Micro-fluidic chip with temperature control function, photochemical micro-nano processing system and method |
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Cited By (16)
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| CN109328229A (en) * | 2016-11-01 | 2019-02-12 | 深圳华大智造科技有限公司 | Gene sequencing chip and its installing frame |
| WO2018081920A1 (en) * | 2016-11-01 | 2018-05-11 | 深圳华大智造科技有限公司 | Gene sequencing chip and mounting frame thereof |
| CN109328229B (en) * | 2016-11-01 | 2021-07-27 | 深圳华大智造科技股份有限公司 | Gene sequencing chip and mounting frame thereof |
| CN111356528A (en) * | 2017-11-22 | 2020-06-30 | 惠普发展公司，有限责任合伙企业 | Multi-region microfluidic devices |
| CN108311177A (en) * | 2018-01-15 | 2018-07-24 | 中国科学院上海微系统与信息技术研究所 | Align structures, alignment assemble method and application thereof for 3D PDMS facture of microchip |
| CN108311177B (en) * | 2018-01-15 | 2020-07-31 | 中国科学院上海微系统与信息技术研究所 | Manufacturing method of 3D PDMS micro-fluidic chip alignment assembly structure |
| CN110387321A (en) * | 2018-04-23 | 2019-10-29 | 深圳华大智造科技有限公司 | Gene sequencing chip and gene sequencing device |
| CN112673090A (en) * | 2018-10-29 | 2021-04-16 | Nok株式会社 | Micro-fluidic chip and micro-fluidic device |
| CN110252436A (en) * | 2019-07-03 | 2019-09-20 | 水利部中国科学院水工程生态研究所 | A kind of detachable planktonic organism micro-fluidic chip for high power micro image collection |
| CN110252436B (en) * | 2019-07-03 | 2024-10-11 | 水利部中国科学院水工程生态研究所 | Detachable plankton micro-fluidic chip for high-power display micro-image acquisition |
| CN110564610B (en) * | 2019-10-15 | 2024-08-23 | 杭州比芯诊断技术有限公司 | Double-temperature-zone PCR amplification device |
| CN110564610A (en) * | 2019-10-15 | 2019-12-13 | 杭州比芯诊断技术有限公司 | Double-temperature-zone PCR amplification device |
| CN112113901A (en) * | 2020-09-18 | 2020-12-22 | 深圳先进技术研究院 | Chip fixing device |
| CN113059511A (en) * | 2021-03-19 | 2021-07-02 | 杭州比芯诊断技术有限公司 | Microfluidic PCR plate fixing device |
| CN118079818B (en) * | 2024-03-06 | 2024-08-13 | 暨南大学 | Micro-fluidic chip with temperature control function, photochemical micro-nano processing system and method |
| CN118079818A (en) * | 2024-03-06 | 2024-05-28 | 暨南大学 | Micro-fluidic chip with temperature control function, photochemical micro-nano processing system and method |
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