CN105772125B - Micro-fluidic chip clamp experiment porch based on 3D printing - Google Patents

Micro-fluidic chip clamp experiment porch based on 3D printing Download PDF

Info

Publication number
CN105772125B
CN105772125B CN201610258731.5A CN201610258731A CN105772125B CN 105772125 B CN105772125 B CN 105772125B CN 201610258731 A CN201610258731 A CN 201610258731A CN 105772125 B CN105772125 B CN 105772125B
Authority
CN
China
Prior art keywords
substrate
micro
fluidic chip
cover board
insert port
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201610258731.5A
Other languages
Chinese (zh)
Other versions
CN105772125A (en
Inventor
范强
范一强
王玫
张亚军
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beijing University of Chemical Technology
Original Assignee
Beijing University of Chemical Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing University of Chemical Technology filed Critical Beijing University of Chemical Technology
Priority to CN201610258731.5A priority Critical patent/CN105772125B/en
Publication of CN105772125A publication Critical patent/CN105772125A/en
Application granted granted Critical
Publication of CN105772125B publication Critical patent/CN105772125B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02Adapting objects or devices to another
    • B01L2200/026Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)

Abstract

The invention discloses a kind of micro-fluidic chip clamp experiment porch based on 3D printing, substrate and cover board two parts are experiment porch agent structure, two parts are bolted, there are the rectangular window of same size and four bolt holes, four rectangular preiection positioning settings for substrate and cover plate central, substrate have ramp type micro-fluidic chip insert port, inside have left and right positioning chuck, cover board each side be distributed there are six connecting tube insert port and sealing ring installation site.There are the rectangular window of same size and four bolt holes, four rectangular preiection positioning settings for substrate cover plate central, substrate has ramp type micro-fluidic chip insert port, inside to have left and right positioning chuck, and each side there are six connecting tube insert port and sealing ring installation sites for distribution on cover board.Device is suitable for all kinds of micro-fluidic chips experiment detection and laboratory uses, and experiment porch rapid processing, cost of manufacture purpose that is cheap, easy to operate, improving experiment connecting tube effect may be implemented.

Description

Micro-fluidic chip clamp experiment porch based on 3D printing
Technical field
The present invention relates to a kind of micro-fluidic chip clamp experiment porch based on 3D printing, specifically, being 3D printing Technology is applied in the making of micro-fluidic experiment porch, realizes the rapid processing of experiment porch, reduces cost of manufacture, is easy to operate Purpose, which is suitable for various biomedical detections and chemical analysis application using micro-fluidic chip, belongs to In microfluidic art.
Background technology
3D printing belongs to one kind of rapid shaping technique, and central principle is:" Layered manufacturing is successively superimposed ".3D printing The printed material of machine is raw material of device, such as metal, ceramics, plastics, sand etc..Specific implementation process is to utilize computer aided manufacturing It helps design or modeling software to commodity modeling, model division is then generated STL standard data files at section successively.3D Printer successively prints and is bonded, produce entity by identifying file middle section information.At present its be widely used in automobile, The fields such as aviation, medical treatment, building.Material is greatly saved in the principle of its increasing material manufacturing, reduces product cost, and product quality is steady It is fixed.But for the very high product of required precision, need subsequently to polish and finish;Because in process, material is heated repeatedly, Therefore interiors of products stress is complicated, product material performance is poor, such as intensity, rigidity etc..So 3D printing for required precision not High, product strength requires also not high product highly advantageous.
Microfluidic chip technology is one of the hot spot of present analysis science research.Micro-fluidic chip is biology, chemistry, doctor The basic operation units such as sample preparation, reaction, separation, the detection of analytic process are learned to be integrated on the chip of one piece of micro-meter scale, It is automatically performed analysis overall process.Microfluidic chip analysis is using chip as operating platform, while based on analytical chemistry, with microcomputer EDM Technology is to rely on, and is current main application with life science using microchannel network as structure feature, is current micro- The emphasis of analysis system field development.And laboratory is needed through connecting tube when carrying out micro-fluidic chip experiment fluid It imports in micro-fluidic chip, therefore experiment is required for connecting connecting tube in each interface every time, while being sealed.It is each micro-fluidic Chip has several interfaces, and it is small to make an excuse size, so take over operation can consume the plenty of time in experiment, and quality of connection with Manual operation stability is related, easily occurs leaking and blocking.The unreasonable arrangement of connecting tube can also influence Germicidal efficacy and result.
Invention content
Micro-fluidic chip connecting tube relies on experimenter's operating experience, time-consuming in order to overcome in existing laboratory work and The cumbersome problem of connection procedure, the present invention provide a kind of micro-fluidic chip clamp experiment porch based on 3D printing, should be based on 3D The micro-fluidic chip clamp experiment porch of printing not only assists in experimenter and quickly connects the company for completing disengaging micro-fluidic chip It takes over, and can guarantee quality of connection, operation is simple.
The technical solution adopted by the present invention to solve the technical problems is:It is of the present invention based on the micro-fluidic of 3D printing Chip fixture experiment porch, including substrate, cover board, bolt connecting hole, substrate and cover board two parts are experiment porch agent structure, Two parts are bolted, substrate and cover plate central there are the rectangular window of same size and four bolt holes, four Rectangular preiection positioning setting, substrate have ramp type micro-fluidic chip insert port, inside to have left and right positioning chuck, the cover board left and right sides There are six connecting tube insert port and sealing ring installation sites for each distribution.
The substrate and cover board is made of 3D printing.
Described connecting tube insert port or so is all provided with six, is arranged according to rectangular mode.
There is ramp type micro-fluidic chip insert port on the substrate.
There are many positioning methods, including rectangular preiection, left and right to position chuck, be bolted for substrate and cover board.
Apparatus of the present invention design is succinct, using 3D printing, realizes that inexpensive rapid processing, ramp type micro-fluidic chip are inserted Entrance allows micro-fluidic chip to be smoothly inserted into, and will not damage, and rectangular preiection, is bolted so that base left and right positioning chuck Plate and cover board are accurately positioned, and multiple connecting tube insert ports can meet the design requirement of different micro-fluidic chips in subsequent experimental.
The invention has the advantages that the process that cumbersome connecting fluid flows into pipe can be saved, it is only necessary to micro-fluidic Chip is inserted into substrate cover upper cover plate and tightens fixing bolt, convenient succinct, ensures the quality of connection of connecting tube.
Description of the drawings
Fig. 1 is the substrate covering plate structure figure of the present invention.
In figure:1, bolt connecting hole, 2, connecting tube insert port, 3, sealing ring, 4, cover board, 5, substrate, 6, rectangular window, 7, Rectangular preiection, 8, left and right positioning chuck, 9, ramp type micro-fluidic chip insert port, 10, rectangular recess.
Specific implementation mode
Present invention will be further explained below with reference to the attached drawings and examples.
In Fig. 1, designed structure is made using 3D printing, cover board (4) is equipped with sealing ring (3), installation site installation Be not easy to be tested the sealing ring of flow-induced corrosion material used, connecting tube be installed in connecting tube insert port (2) position, substrate (5) with Cover board (4) is aligned, and rectangular preiection (7) is aligned with rectangular recess (10);After bolt is connect with bolt connecting hole (1), substrate (5) and Cover board (4) is mutually fixed;When experiment, micro-fluidic chip is entered from ramp type micro-fluidic chip insert port (9), is positioned using left and right Chuck (8) positions, and rectangular window (6) can observe fluid state in micro-fluidic chip, can also be placed directly within microscopically observation, ties Beam can pop up micro-fluidic chip when testing from bottom rectangle window (6).

Claims (2)

1. the micro-fluidic chip clamp experiment porch based on 3D printing, it is characterised in that:The platform includes substrate, cover board, bolt Connecting hole, substrate and cover board two parts are experiment porch agent structure, and two parts are bolted, and substrate and cover plate central stay The rectangular window and four bolt holes, four rectangular preiection positioning settings, substrate for having same size have ramp type micro-fluidic Chip insert port, inside have left and right positioning chuck, cover board each side be distributed there are six connecting tube insert port and sealing ring peace Holding position;
The substrate and cover board is made of 3D printing;
Described connecting tube insert port or so is all provided with six, is arranged according to rectangular mode;
There is ramp type micro-fluidic chip insert port on the substrate;
Cover board (4) is equipped with sealing ring (3), and installation site installation is not easy to be tested the sealing ring of flow-induced corrosion material used, Connecting tube is installed in connecting tube insert port (2) position, and substrate (5) is aligned with cover board (4), rectangular preiection (7) and rectangular recess (10) Alignment;After bolt is connect with bolt connecting hole (1), substrate (5) is mutually fixed with cover board (4);When experiment, micro-fluidic chip from oblique Shelving micro-fluidic chip insert port (9) enters, and is positioned using left and right positioning chuck (8), and rectangular window (6) can observe micro-fluidic core Fluid state in piece can also be placed directly within microscopically observation, can be popped up from bottom rectangle window (6) when terminating experiment micro-fluidic Chip.
2. the micro-fluidic chip clamp experiment porch according to claim 1 based on 3D printing, it is characterised in that:Substrate and There are many positioning methods, including rectangular preiection, left and right to position chuck, be bolted for cover board.
CN201610258731.5A 2016-04-23 2016-04-23 Micro-fluidic chip clamp experiment porch based on 3D printing Active CN105772125B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610258731.5A CN105772125B (en) 2016-04-23 2016-04-23 Micro-fluidic chip clamp experiment porch based on 3D printing

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610258731.5A CN105772125B (en) 2016-04-23 2016-04-23 Micro-fluidic chip clamp experiment porch based on 3D printing

Publications (2)

Publication Number Publication Date
CN105772125A CN105772125A (en) 2016-07-20
CN105772125B true CN105772125B (en) 2018-09-21

Family

ID=56399384

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610258731.5A Active CN105772125B (en) 2016-04-23 2016-04-23 Micro-fluidic chip clamp experiment porch based on 3D printing

Country Status (1)

Country Link
CN (1) CN105772125B (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109328229B (en) * 2016-11-01 2021-07-27 深圳华大智造科技股份有限公司 Gene sequencing chip and mounting frame thereof
WO2019103732A1 (en) * 2017-11-22 2019-05-31 Hewlett-Packard Development Company, L.P. Multizonal microfluidic devices
CN108311177B (en) * 2018-01-15 2020-07-31 中国科学院上海微系统与信息技术研究所 Manufacturing method of 3D PDMS micro-fluidic chip alignment assembly structure
CN110387321B (en) * 2018-04-23 2023-03-17 深圳华大智造科技股份有限公司 Gene sequencing chip and gene sequencing device
WO2020090481A1 (en) * 2018-10-29 2020-05-07 Nok株式会社 Micro-fluid chip and micro-fluid device
CN110252436A (en) * 2019-07-03 2019-09-20 水利部中国科学院水工程生态研究所 A kind of detachable planktonic organism micro-fluidic chip for high power micro image collection
CN110564610A (en) * 2019-10-15 2019-12-13 杭州比芯诊断技术有限公司 Double-temperature-zone PCR amplification device
CN112113901A (en) * 2020-09-18 2020-12-22 深圳先进技术研究院 Chip fixing device
CN113059511A (en) * 2021-03-19 2021-07-02 杭州比芯诊断技术有限公司 Microfluidic PCR plate fixing device

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008039875A1 (en) * 2006-09-28 2008-04-03 California Institute Of Technology System and method for interfacing with a microfluidic chip
CN201222052Y (en) * 2008-06-20 2009-04-15 浙江大学 Device for clamping micro-fluidic chip
CN102943665A (en) * 2012-11-08 2013-02-27 西安信唯信息科技有限公司 Real-time observation mechanism of flowing of fluid in porous medium
CN103058131A (en) * 2012-12-19 2013-04-24 中国科学院上海微系统与信息技术研究所 Manufacture method of reversible bonding micro-fluidic chip with high strength
CN103386345A (en) * 2013-08-07 2013-11-13 苏州扬清芯片科技有限公司 Micro-fluidic chip clamp
CN105344392A (en) * 2015-12-07 2016-02-24 苏州汶颢芯片科技有限公司 Fixture for microfluidic chip of sandwich structure
CN105396633A (en) * 2015-12-22 2016-03-16 苏州汶颢芯片科技有限公司 Soft microfluidic chip reversible clamp
CN105457695A (en) * 2016-01-12 2016-04-06 苏州汶颢芯片科技有限公司 Reversible clamp for micro-fluidic chip

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2167233B1 (en) * 2007-06-26 2013-01-23 Micronit Microfluidics B.V. Device and method for fluidic coupling of fluidic conduits to a microfluidic chip, and uncoupling thereof
FR2951153A1 (en) * 2009-10-09 2011-04-15 Corning Inc MICROFLUIDIC DEVICE
DE102010037532A1 (en) * 2010-09-14 2012-03-15 Andreas Hettich Gmbh & Co. Kg Connecting device for fluidic contacting of microfluidic chips
KR101737121B1 (en) * 2010-12-21 2017-05-17 엘지전자 주식회사 Microfluidic system

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008039875A1 (en) * 2006-09-28 2008-04-03 California Institute Of Technology System and method for interfacing with a microfluidic chip
CN201222052Y (en) * 2008-06-20 2009-04-15 浙江大学 Device for clamping micro-fluidic chip
CN102943665A (en) * 2012-11-08 2013-02-27 西安信唯信息科技有限公司 Real-time observation mechanism of flowing of fluid in porous medium
CN103058131A (en) * 2012-12-19 2013-04-24 中国科学院上海微系统与信息技术研究所 Manufacture method of reversible bonding micro-fluidic chip with high strength
CN103386345A (en) * 2013-08-07 2013-11-13 苏州扬清芯片科技有限公司 Micro-fluidic chip clamp
CN105344392A (en) * 2015-12-07 2016-02-24 苏州汶颢芯片科技有限公司 Fixture for microfluidic chip of sandwich structure
CN105396633A (en) * 2015-12-22 2016-03-16 苏州汶颢芯片科技有限公司 Soft microfluidic chip reversible clamp
CN105457695A (en) * 2016-01-12 2016-04-06 苏州汶颢芯片科技有限公司 Reversible clamp for micro-fluidic chip

Also Published As

Publication number Publication date
CN105772125A (en) 2016-07-20

Similar Documents

Publication Publication Date Title
CN105772125B (en) Micro-fluidic chip clamp experiment porch based on 3D printing
JP6654681B2 (en) Single-structure biochip and process for providing process from sample introduction to result output
US8617489B2 (en) Microfluidic interface
CN107904156A (en) A kind of one full-automation digital pcr detecting system and implementation
CN104941703B (en) Micro-fluidic chip method is prepared using 3D printing template and modularization assembling
CN207933420U (en) A kind of micro-fluidic chip of genetic test
Gupta et al. 3D printing in chemical sciences: applications across chemistry
CN105349401A (en) Multifunctional integrated microfluidic nucleic acid analysis chip and preparation and analysis method thereof
CN104549591B (en) A kind of general device fixed and connect belt electrode micro-fluidic chip
CN105749994B (en) A kind of three-dimensional microflow control chip preparation method of plural layers fitting
Zhuang et al. A fully integrated and automated microsystem for rapid pharmacogenetic typing of multiple warfarin-related single-nucleotide polymorphisms
CN108593950A (en) A kind of liquid automatic sample feeding device
WO2016044244A1 (en) Apparatus and methods for manufacturing a microfluidic device
Dalton et al. A cost effective, re-configurable electrokinetic microfluidic chip platform
CN103252262A (en) Polymethylmethacrylate material micro-fluidic chip processing technology
AU2013204387B2 (en) Unitary biochip providing sample-in to results-out processing and methods of manufacture
Kong et al. Label-free counting of affinity-enriched circulating tumor cells (CTCs) using a thermoplastic micro-C oulter counter (μCC)
Schneider et al. Integrated magneto–electrophoresis microfluidic chip purification on library preparation device for preimplantation genetic testing for aneuploidy detection
Reichen et al. Characterisation of an adhesive-free packaging system for polymeric microfluidic biochemical devices and reactors
AU2016200080B2 (en) Unitary biochip providing sample-in to results-out processing and methods of manufacture
CN209024559U (en) A kind of 48 hole deep-well plates extracted for full-automatic DNA
Zhao et al. Microfluidic gasketless interconnects sealed by superhydrophobic surfaces
CN206298576U (en) A kind of reagent disc that pre-treatment is automated for detection of nucleic acids
CN206038516U (en) High flux real -time detection system is united to optoacoustic
CN108187767A (en) A kind of programmable modularized PDMS Micro-fluidic chip die systems

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant