CN103162996A - Pretreatment method for automatic identification of fruit fly images - Google Patents
Pretreatment method for automatic identification of fruit fly images Download PDFInfo
- Publication number
- CN103162996A CN103162996A CN2011104129342A CN201110412934A CN103162996A CN 103162996 A CN103162996 A CN 103162996A CN 2011104129342 A CN2011104129342 A CN 2011104129342A CN 201110412934 A CN201110412934 A CN 201110412934A CN 103162996 A CN103162996 A CN 103162996A
- Authority
- CN
- China
- Prior art keywords
- sample
- wing
- filter paper
- trypetid
- automatic identification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a pretreatment method for automatic identification of fruit fly images, and relates to a pretreatment method for identification of an image combining a novel preparation of fruit fly specimens with image digitization. The treatment method comprises the steps of softening the specimens-performing pretreatment-spreading wings-acquiring a whole attitude image. The pretreatment method for automatic identification of fruit fly images combining the preparation of fruit fly specimens with the image digitization has no damage to specimens and is easy for standardization. Image quality meets the requirements of image automatic identification.
Description
Technical field
The present invention relates to trypetid image automatic identification pre-treating method, be specifically related to the image recognition pre-treating method that a kind of new trypetid preparation of specimen combines with image digitazation.
Background technology
In the research of the automatic authenticate technology of insect image identification, want to obtain qualification result accurately, picture quality there is certain requirement.Therefore the Sample pretreatment technology plays a part crucial.
Traditional cushion mark easily damages sample, and is complicated and be not easy sample is carried out standardization, and figure is unintelligible, and quality is not fine.
?
Summary of the invention
The purpose of this invention is to provide trypetid image automatic identification pre-treating method, the trypetid image automatic identification pretreatment technology that it adopts preparation of specimen to combine with image digitazation, to the sample not damaged, easily standardization, picture quality satisfies the needs of image automatic identification.
In order to solve the existing problem of background technology, the present invention is by the following technical solutions: its disposal route is: whole appearance image acquisition that sample eases back-pre-treatment--spreading the wings--.
It is that sample is placed in the device that eases back 2-3 days that described sample eases back.The wet sand that the device underneath 50% that eases back is alcohol-pickled.In the process that eases back, available tweezers pull foot and wing, judge whether to ease back successfully.
Described pre-treatment is the sample after easing back to be put into 50% alcohol embathe 3min, get rid of foreign material and bubble on polypide, taking-up is put and is soaked 3～5h. in 50% glycerine of people 1:1+5% NaOH mixed aqueous solution and coordinate with tweezers and elbow dissecting needle in solution the worm wing is pulled to polypide the place ahead, make wing base section can activity freely, make the joint motion of feeler, abdomen base portion and foot.Embathe with clear water after taking out.
Described spreading the wings is that a filter paper is open and flat in double dish, drenched with distilled water. drain excessive moisture, with tweezers, the trypetid adult back side is lain on wet filter paper down, drawing 50% ethanol number droplet drips in polypide, the worm wing stretched and be close to filter paper, with the fixing polypide of the bent face of an elbow dissecting needle, with another elbow dissecting needle bent face, the push-and-pull of worm wing being flattened, two wings are opened, and the wing upper limb is as far as possible in alignment.What of dropping liquid are convenient to expand as suitable take the worm wing.Determine must stop fixing a moment by dissecting needle behind the position, treat that dropping liquid all infiltrates filter paper, the worm wing just is fixed on filter paper naturally.Two filter paper are open and flat in double dish covers, and water is drenched with filter paper, drains excessive moisture, and on the trypetid sample that Gai Zaiyi is spread the wings, under normal temperature, moisturizing is fixed 2～3 days,
Described whole appearance is after spreading the wings, and carries out whole appearance with small size tweezers and dissection for sample.Should guarantee head up, foot can not block wing as far as possible.Abdomen end as far as possible upwards guarantees to overlook to see the whole speckles of belly.Allow sample naturally dry.
Described image acquisition is after sample dries, with the suction of the insect needle perpendicular alignmnet sample chest outside of belly.The degree of depth of contact pin will make sample be fixed on insect needle, but pin can not penetrate chest.After being plugged, upwards carry insect needle, the sample wing is come off from filter paper.The insect needle other end vertically is inserted on the foam plastic magazine, is placed on rephotograph stand, wait to take pictures; Turn on the lamp of rephotograph stand both sides, adjust angle, eliminate the shadow of sample.Fixedly camera, mix up acquisition parameters.The parameter of camera is made as: resolution 3264*2448, ISO80, macro mode, time shutter 1/60.Be noted that acquisition parameters, the height of camera, and the distance of camera from camera lens from sample should be consistent.
The present invention has following beneficial effect: the trypetid image automatic identification pretreatment technology that adopts preparation of specimen to combine with image digitazation, and to the sample not damaged, easily standardization, picture quality satisfies the needs of image automatic identification.
Embodiment:
This embodiment is by the following technical solutions: its disposal route is: whole appearance image acquisition that sample eases back-pre-treatment--spreading the wings--.
It is that sample is placed in the device that eases back 2-3 days that described sample eases back.The wet sand that the device underneath 50% that eases back is alcohol-pickled.In the process that eases back, available tweezers pull foot and wing, judge whether to ease back successfully.
Described pre-treatment is the sample after easing back to be put into 50% alcohol embathe 3min, get rid of foreign material and bubble on polypide, taking-up is put and is soaked 3～5h. in 50% glycerine of people 1:1+5% NaOH mixed aqueous solution and coordinate with tweezers and elbow dissecting needle in solution the worm wing is pulled to polypide the place ahead, make wing base section can activity freely, make the joint motion of feeler, abdomen base portion and foot.Embathe with clear water after taking out.
Described spreading the wings is that a filter paper is open and flat in double dish, drenched with distilled water. drain excessive moisture, with tweezers, the trypetid adult back side is lain on wet filter paper down, drawing 50% ethanol number droplet drips in polypide, the worm wing stretched and be close to filter paper, with the fixing polypide of the bent face of an elbow dissecting needle, with another elbow dissecting needle bent face, the push-and-pull of worm wing being flattened, two wings are opened, and the wing upper limb is as far as possible in alignment.What of dropping liquid are convenient to expand as suitable take the worm wing.Determine must stop fixing a moment by dissecting needle behind the position, treat that dropping liquid all infiltrates filter paper, the worm wing just is fixed on filter paper naturally.Two filter paper are open and flat in double dish covers, and water is drenched with filter paper, drains excessive moisture, and on the trypetid sample that Gai Zaiyi is spread the wings, under normal temperature, moisturizing is fixed 2～3 days,
Described whole appearance is after spreading the wings, and carries out whole appearance with small size tweezers and dissection for sample.Should guarantee head up, foot can not block wing as far as possible.Abdomen end as far as possible upwards guarantees to overlook to see the whole speckles of belly.Allow sample naturally dry.
Described image acquisition is after sample dries, with the suction of the insect needle perpendicular alignmnet sample chest outside of belly.The degree of depth of contact pin will make sample be fixed on insect needle, but pin can not penetrate chest.After being plugged, upwards carry insect needle, the sample wing is come off from filter paper.The insect needle other end vertically is inserted on the foam plastic magazine, is placed on rephotograph stand, wait to take pictures; Turn on the lamp of rephotograph stand both sides, adjust angle, eliminate the shadow of sample.Fixedly camera, mix up acquisition parameters.The parameter of camera is made as: resolution 3264*2448, ISO80, macro mode, time shutter 1/60.Be noted that acquisition parameters, the height of camera, and the distance of camera from camera lens from sample should be consistent.
The trypetid image automatic identification pretreatment technology that this embodiment adopts preparation of specimen to combine with image digitazation, to the sample not damaged, easily standardization, picture quality satisfies the needs of image automatic identification.
Claims (6)
1. trypetid image automatic identification pre-treating method, is characterized in that its disposal route is: whole appearance image acquisition that sample eases back-pre-treatment--spreading the wings--.
2. trypetid image automatic identification pre-treating method according to claim 1 is characterized in that it is that sample is placed in the device that eases back 2-3 days that described sample eases back; The wet sand that the device underneath 50% that eases back is alcohol-pickled; In the process that eases back, available tweezers pull foot and wing, judge whether to ease back successfully.
3. trypetid image automatic identification pre-treating method according to claim 1, it is characterized in that described pre-treatment is the sample after easing back to be put into 50% alcohol embathe 3min, get rid of foreign material and bubble on polypide, take out in 50% glycerine of putting people 1:1+5% NaOH mixed aqueous solution and soak 3～5h; Coordinate with tweezers and elbow dissecting needle the worm wing is pulled to polypide the place ahead in solution, make wing base section can activity freely, make the joint motion of feeler, abdomen base portion and foot; Embathe with clear water after taking out.
4. trypetid image automatic identification pre-treating method according to claim 1, it is characterized in that described spreading the wings is that a filter paper is open and flat in double dish, drenched with distilled water. drain excessive moisture, with tweezers, the trypetid adult back side is lain on wet filter paper down, drawing 50% ethanol number droplet drips in polypide, the worm wing is stretched and be close to filter paper, with the fixing polypide of the bent face of an elbow dissecting needle, with another elbow dissecting needle bent face, the push-and-pull of worm wing is flattened, two wings are opened, and the wing upper limb is as far as possible in alignment; What of dropping liquid are convenient to expand as suitable take the worm wing; Determine must stop fixing a moment by dissecting needle behind the position, treat that dropping liquid all infiltrates filter paper, the worm wing just is fixed on filter paper naturally; Two filter paper are open and flat in double dish covers, and water is drenched with filter paper, drains excessive moisture, and on the trypetid sample that Gai Zaiyi is spread the wings, under normal temperature, moisturizing is fixed 2～3 days.
5. trypetid image automatic identification pre-treating method according to claim 1, is characterized in that described whole appearance is after spreading the wings, and carries out whole appearance with small size tweezers and dissection for sample; Should guarantee head up, foot can not block wing as far as possible; Abdomen end as far as possible upwards guarantees to overlook to see the whole speckles of belly; Allow sample naturally dry.
6. trypetid image automatic identification pre-treating method according to claim 1, is characterized in that described image acquisition is after sample dries, with the suction of the insect needle perpendicular alignmnet sample chest outside of belly; The degree of depth of contact pin will make sample be fixed on insect needle, but pin can not penetrate chest; After being plugged, upwards carry insect needle, the sample wing is come off from filter paper; The insect needle other end vertically is inserted on the foam plastic magazine, is placed on rephotograph stand, wait to take pictures; Turn on the lamp of rephotograph stand both sides, adjust angle, eliminate the shadow of sample; Fixedly camera, mix up acquisition parameters; The parameter of camera is made as: resolution 3264*2448, ISO80, macro mode, time shutter 1/60; Be noted that acquisition parameters, the height of camera, and the distance of camera from camera lens from sample should be consistent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104129342A CN103162996A (en) | 2011-12-13 | 2011-12-13 | Pretreatment method for automatic identification of fruit fly images |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011104129342A CN103162996A (en) | 2011-12-13 | 2011-12-13 | Pretreatment method for automatic identification of fruit fly images |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103162996A true CN103162996A (en) | 2013-06-19 |
Family
ID=48586263
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011104129342A Pending CN103162996A (en) | 2011-12-13 | 2011-12-13 | Pretreatment method for automatic identification of fruit fly images |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103162996A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3874094A (en) * | 1974-02-06 | 1975-04-01 | Walter J Mackey | Preparation of skeletal display of animals |
| JP2002012501A (en) * | 2000-04-27 | 2002-01-15 | Nippon Flour Mills Co Ltd | Specimen of arthropod, method for making specimen and kit for making specimen |
| US20030026484A1 (en) * | 2001-04-27 | 2003-02-06 | O'neill Mark | Automated image identification system |
| US20050025357A1 (en) * | 2003-06-13 | 2005-02-03 | Landwehr Val R. | Method and system for detecting and classifying objects in images, such as insects and other arthropods |
| CN1918983A (en) * | 2006-09-12 | 2007-02-28 | 山西大学 | Production method of insect specimen |
-
2011
- 2011-12-13 CN CN2011104129342A patent/CN103162996A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3874094A (en) * | 1974-02-06 | 1975-04-01 | Walter J Mackey | Preparation of skeletal display of animals |
| JP2002012501A (en) * | 2000-04-27 | 2002-01-15 | Nippon Flour Mills Co Ltd | Specimen of arthropod, method for making specimen and kit for making specimen |
| US20030026484A1 (en) * | 2001-04-27 | 2003-02-06 | O'neill Mark | Automated image identification system |
| US20050025357A1 (en) * | 2003-06-13 | 2005-02-03 | Landwehr Val R. | Method and system for detecting and classifying objects in images, such as insects and other arthropods |
| CN1918983A (en) * | 2006-09-12 | 2007-02-28 | 山西大学 | Production method of insect specimen |
Non-Patent Citations (4)
| Title |
|---|
| WANG LU ET AL.: "Research on landmark extraction technology in identification of fruit flies（Diptera：Tephritidae）", 《2011 SEVENTH INTERNATIONAL CONFERENCE ON NATURAL COMPUTATION》 * |
| 唐立俊: "昆虫标本制作综述", 《遵义师专学报》 * |
| 常征: "昆虫标本制作方法新探", 《文山师范高等专科学校学报》 * |
| 黄世水: "实蝇成虫针插标本的整姿和制作", 《植物检疫》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ECSP21088851A (en) | AQUATIC ANIMAL CULTURE SUPPORT SYSTEM, LIFTING APPARATUS, FEEDING APPARATUS, AQUATIC ANIMAL CULTURE METHOD AND AQUATIC ANIMAL CULTURE SUPPORT PROGRAM | |
| CN202998870U (en) | Tea picking system | |
| JP2018000280A5 (en) | Drawing device | |
| CN102629999A (en) | Automatic bedtime television shutdown service based on eye state identification technology | |
| CN204442575U (en) | Hydrophobicity of Composite Insulator image collecting device | |
| CN103162996A (en) | Pretreatment method for automatic identification of fruit fly images | |
| CN205307143U (en) | Little mice live body fixing device for pathology experiments | |
| CN104323870A (en) | Rabbit fixing box for skin stimulus response test and using method for rabbit fixing box | |
| CN204233214U (en) | A kind of laryngomicrosurgery nipper with suction pump | |
| CN103735250A (en) | Small animal tongue-picture photographing method | |
| CN204667426U (en) | Iris image acquisition platform | |
| CN102721583A (en) | Method for producing film of leaf epidermis of stomatal pit of sweetscented oleander leaf | |
| CN103085585B (en) | Method for extracting leaf vein by using leaf | |
| CN204695339U (en) | A kind of fingerprint refers to venous collection device | |
| CN208191970U (en) | It is a kind of for capturing the capture device of flying insect alive | |
| CN204176265U (en) | A kind of replaceable photographic goods support from racket head | |
| CN203841835U (en) | Medical experimental animal fixing device | |
| CN205322337U (en) | Draw operating means of two shell shellfish hemolymphs | |
| ATE473682T1 (en) | DEVICE FOR MINIMALLY INVASIVE BLOOD COLLECTION FROM ANIMALS USING BLOOD-SAFE PREDATOR BUGS | |
| CN207041592U (en) | Novel veterinarian fixing device | |
| CN204880978U (en) | Take off cell amnion freeze -drying device | |
| CN203662735U (en) | Tongue picture photographing device | |
| CN203027887U (en) | Animal feeding device for study of fowl coccidiosis | |
| Dandan et al. | The CPH and the optimal age of child second language acquisition | |
| CN208254823U (en) | A kind of biological evidence extraction cotton swab set |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20130619 |
|
| RJ01 | Rejection of invention patent application after publication |