CN103156188A - Health food with function of immune regulation and hypoxia tolerance - Google Patents
Health food with function of immune regulation and hypoxia tolerance Download PDFInfo
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- CN103156188A CN103156188A CN2011104197937A CN201110419793A CN103156188A CN 103156188 A CN103156188 A CN 103156188A CN 2011104197937 A CN2011104197937 A CN 2011104197937A CN 201110419793 A CN201110419793 A CN 201110419793A CN 103156188 A CN103156188 A CN 103156188A
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Abstract
The invention provides a health food with functions of immune regulation and hypoxia tolerance. The health food provided by the invention comprises the following ingredients by weight percentage: 2%-3% of a Ganoderma lucidum sporocarp extract, 3%-4% of an Astragalus extract, 2%-3% of a medlar extract, 2%-3% of a ginkgo leaf extract, 40%-45% of ginseng, 34%-36% of taurine, 3%-4% of natural vitamin E, 3%-5% of stable vitamin C and 1%-2% of magnesium stearate. Proved by the functional test, each dosage group of the health food provided by the invention can enhance delayed hypersensitivity of mice (P<0.05), enhance of ConA induced mice lymphocyte transformation ability (P<0.05), obviously prolong hypoxia tolerance survival time (P<0.05) of test mice under ordinary pressure, prolong survival time of the mice after sodium nitrite poisoning (P<0.05, P<0.01), and enhance hypoxia tolerance of the test mice in the condition of sodium nitrite poisoning. The product provided by the invention is capable of regulating immunity and improving hypoxia tolerance ability by multiple mechanisms and links, and has great application value in maintaining and improving the health of people.
Description
Technical field
The present invention relates to health products, be specifically related to a kind of health food with immunological regulation, resisting oxygen lack.
Background technology
Modern times, immunology was thought, immune system has immune defense, immunological homeostasis and the large function of immunosurveillance three, and by these functions, resisted outer heresy, and heresy and immunological regulation in removing are safeguarded the balance of body and stablize.Immune system is a complexity, sensitivity, the orderly network system, and any one link damaged or extremely all can cause immune defense function impaired shows as resistance against diseases and descend, and easily suffers from infectious diseases etc.Therefore, safeguard that the normal physiological function of immune system is particularly important to prevention of diseae.
Anoxic refers to that the required oxygen of body vital movement can not obtain sufficient supply, is a kind of pessimal stimulation to body, can bring out each systemic-function of body and change, and affects organism metabolism, threatens health.For example, large cerebral anoxia, the power that can arouse attention are not concentrated, dizziness, tinnitus, ability to work decline.When the body anoxic, have the antioxidase synthesis capability generation obstacle of removing oxygen radical in body, the content of superoxide dismutase (SOD), glutathione peroxidase (GPX) etc. is reduced, cause accumulating of oxygen radical.Meanwhile, anoxic makes and produces a large amount of free radicals in body, and biomembrane lipid peroxidation strengthens.
Research is found, many traditional tonic medicines have anti-infective, antiviral, antitumorly as the Radix Astragali, glossy ganoderma, matrimony vine etc., and most Chinese medicine has dual regulation to the immune system of human body.Also there is certain impact in anoxic body free radical system, and its active ingredient such as panaxoside, the total ketone of the Radix Astragali and polysaccharide etc. have the ability of good removing free radical, can prevent the peroxidating of biomembrane lipid, alleviate the peroxide injury of body.
Suitably taking nutritious supplementary pharmaceutical, is to safeguard immune balance, improves one of important measures of body's hypoxia tolerance, and Chinese medicine is being regulated immunity, and there is advantageous advantage anti-anoxic aspect.The nutrient such as vitamin, amino acid anti-stress aspect also brought into play important function.Therefore, utilize the drug matching antioxidant, exploitation has the immunity of adjusting, and the nutriment of the double effects of anti-the anoxic has positive role for promoting health of people.
Summary of the invention
Technical problem to be solved by this invention is that research and design has the compound functional preparation of immunoregulation effect and resisting oxygen lack.
The invention provides a kind of health food (commodity are doctor's board match promise recovering capsule difficult to understand) with immunological regulation, resisting oxygen lack
The health food that the present invention has immunological regulation, a resisting oxygen lack is grouped into by the one-tenth of following weight percent proportioning:
Health food of the present invention is grouped into by the one-tenth of following weight proportion:
Health food of the present invention is grouped into by the one-tenth of following weight proportion:
Health food of the present invention is grouped into by the one-tenth of following weight proportion:
Raw material of the present invention is commercially available acquisition all.
The effect of each component of health food of the present invention is as follows:
Glossy ganoderma (Ganoderma lucidum) is the very high famous and precious fungi of a kind of medical value, studies have shown that the ganoderma active material has multiple pharmacologically active, as strengthening immunity, delays senility, and is antitumor, antiviral, protects the liver and protects courage, blood lipid-reducing blood sugar-decreasing etc.Polysaccharide compound is one of contained main chemical compositions of glossy ganoderma, and it can act on a plurality of links of Organism immunoregulation, comprises antigen presenting cell, T cell, B cell, macrophage and N K cell etc.GL-B is the enhanced machine body immunity function significantly, as strengthening the mouse delayed hypersensitivity, promote lymphproliferation response, strengthen the cytotoxic cell function, strengthen macrophage phagocytic function, strengthening the cytotoxic activity of NK cell and the generation that promotes cell factor.
The Radix Astragali is traditional traditional tonic medicine, begins to be stated from Eastern Han Dynasty's Shennong's Herbal, is listed as top grade.Note in Compendium of Material Medica " Radix Astragali is sweet, and the flavor tepor enters spleen, returns lung channel, has the effect that positive strengthening exterior and reducing sweat, promoting pus discharge and tissue regeneration and sharp water detumescence are given birth in tonifying Qi ".Modern pharmacological research shows, the Radix Astragali contains the trace elements such as Astrageloside, polysaccharide, Flavonoid substances and several amino acids and selenium, silicon, cobalt, molybdenum, other compositions also comprise the number of chemical compositions such as cumarin, folic acid, picric acid, choline, caraway alkali, linoleic acid, cupreol, have immunological regulation, anti-inflammatory, the multiple pharmacological effect such as anti-oxidant, can strengthen regulating action from many levels performance immunity.It both can directly affect the intracellular matter metabolism, but the inducing machine body cell produces relevant humoral factor again.It can not only strengthen the specific immunity of body, and can strengthen the nospecific immunity of body; It both can strengthen the immunologic function of normal body, can regulate again the abnormal immunologic function of body.
Matrimony vine is also traditional traditional tonic medicine, has the effect of nourishing liver spleen, benefiting shrewd head.In recent years medical research shows, contain multiple nutritional components and trace element in matrimony vine, LBP-X is main bioactive ingredients wherein, to humoral immunity, cellular immunity, facilitation is arranged, can promote mononuclear macrophage, the NK cell, the plaque forming cells, BMDC, the Function of cell factor plays a part positive in expression of receptor, signal conduction.
Ginkgo biloba p.e is a kind of active material with extensive biological function.From the sixties in 20th century, Chinese scholars has been carried out large quantity research to the chemical composition of ginkgo leaf, pharmacological action etc., has confirmed that ginkgo leaf and extract thereof (EGB) have the circulation of the cardiovascular and cerebrovascular of improvement, reduce blood fat, improve the effects such as immunity of organisms, anti-inflammation, resisting oxidation and delaying senility.EGB can not only strengthen the nospecific immunity of body, and specific immunity that can also the enhanced machine body.Simultaneously, EGB both can strengthen the immunologic function of normal body, but the immunologic function of dysregulation body again.
American Ginseng is the araliaceae ginseng plant, and popular name U.S. ginseng, American ginseng, main product are at America ﹠ Canada, and medicinal part is dry root.The American Ginseng function can be nourishing Yin and falling fire take help as main, nourishing generate fluid, the intelligence development of allaying excitement.Modern study shows, American Ginseng has central nervous system inhibitory action and anti-anoxic, antifatigue, anti-ly stress, protection hepatic injury function, strengthen the effects such as immunity, hemostasis, antithrombotic.Ginsenoside is the main active of American Ginseng, has effect anti-oxidant and the inhibition vomiting as the ginsenoside Re, and the caffeic acid of other composition such as Polyphenols and chlorogenic acid help to protect the cardiac muscle cell.
Taurine is the beta-amino ethyl sulfonic acid with extensive BA; be distributed in each tissue in the humans and animals body; it not only participates in the growth metabolism of body as a kind of nutriment; and indirectly participate in keeping and improving the immunologic function of body; as antioxidant and membrane stabilizer protection body cell, regulate neuroendocrine system.
Vitamin C, vitamin E are effective antioxidants, can effectively remove oxygen radical, and stabilizing cell membrane prevents the oxidative damage of immunocyte, recovers the physiological function of impaired immunocyte, and immunocyte is had significant protective effect.
Health food with immunoregulation effect, resisting oxygen lack of the present invention prepares by following method:
Get fruiting body extract of Ganoderma Lucidum, Astragalus Root P.E, wolfberry fruit extract, ginkgo biloba p.e, American Ginseng (powder), taurine, natural VE, stable vitamin C; Get dolomol, mix with above-mentioned raw materials, homogeneous with colloid mill, as capsule 's content, insert in capsulae vacuus.
Health food of the present invention is the compound functional preparation of the compatibilities such as fruiting body extract of Ganoderma Lucidum, Astragalus Root P.E, wolfberry fruit extract, Chinese medicine compound prescription has been inherited tcm characteristic, has traditional Chinese medicine monomer or the not available advantage of single medicinal material aspect immunological regulation and anti-anoxic.Taurine, natural VE and vitamin C are the nutrient of needed by human; be again good antioxidant, have the Cell protection normal function, the effect of anti-oxidation stress; work in coordination with the performance immunological regulation, alleviate oxidative damage with compound Chinese medicine extract, improve the effect of body's hypoxia tolerance.
Prove through functional trial, each dosage group of health food of the present invention can strengthen the Delayed onset allergy (P＜0.05) of mouse.The carbon of mouse lymphocyte conversion capability (P＜0.05) mouse that enhancing ConA induces is cleaned up ability (P＜0.05), raising mouse antibodies cellulation number (P＜0.05), raising mouse HD50 value (P＜0.05) mouse macrophage engulfs chicken erythrocyte phagocytic rate (P＜0.05) and mouse macrophage is engulfed chicken erythrocyte phagocytic index (P＜0.05), strengthens NK cytoactive (P＜0.05) (seeing Table 1～table 4).Can extend significantly the anti-the survival time under hypoxic condition of normal pressure (P＜0.05) of tested mouse.On the anti-anoxia ability impact under tested mouse broken end cerebral anoxia condition significantly (P＜0.01), and the anti-anoxic effect of middle dosage group the most obviously (P＜0.01).Can obviously extend the poisoning rear time-to-live of tested mouse natrium nitrosum (P＜0.05, P＜0.01), improve the anti-anoxia ability (see Table 5-table 7) of tested mouse under the poisoning condition of natrium nitrosum.
Product of the present invention is a kind of by number of mechanisms too many levels adjusting immunity, raising hypoxia-bearing capability, effect is significant, has overcome the existing product mechanism of action single, and effect is single, the deficiency that effect is not good enough is having larger using value aspect maintenance and raising health of people.
The specific embodiment
Following raw material is commercially available acquisition all.Wherein (1) fruiting body extract of Ganoderma Lucidum, Astragalus Root P.E, wolfberry fruit extract are available from the four seas, Nantong plant essence Co., Ltd (polyoses content 〉=50%); Ginkgo biloba p.e (GINKGO BILOBA EXTRACT 〉=24%); American Ginseng (total saponin content 5～10%) (2) natural VE available from DSM company (3) taurine available from Yongan, Qianjiang pharmaceutcal corporation, Ltd (99% content); (4) V
CAvailable from the stone medicine medicine company (99% content) of supporting one's family
Embodiment 1. has the health food (doctor's board match promise recovering capsule difficult to understand) of immunological regulation, resisting oxygen lack
Formula:
Preparation:
Get fruiting body extract of Ganoderma Lucidum 69g, Astragalus Root P.E 80.5g, wolfberry fruit extract 69g; ginkgo biloba p.e 57.5g, American Ginseng 966g, taurine 828g; natural VE 92g; stability vitamin C 103.5g crosses respectively 80 mesh sieves, mixes; add dolomol 34.5g; colloid mill is homogeneous, (model NJP 1200) filling capsulae vacuus according to a conventional method on full-automatic hard shell capsules filling machine, then make 10000 capsules through madreporic vesicle, polishing, sealing process.Every capsules 230mg.
Embodiment 2. has the health food (doctor's board match promise recovering capsule difficult to understand) of immunological regulation, resisting oxygen lack
Formula:
The preparation method makes 10000 capsules with embodiment 1.
Embodiment 3. has the health food (doctor's board match promise recovering capsule difficult to understand) of immunological regulation, resisting oxygen lack
Formula:
The preparation method makes 10000 capsules with embodiment 1.
Embodiment 4. has the function of health food experiment (zoopery) of immunological regulation, resisting oxygen lack
The health food with immunological regulation, resisting oxygen lack that sample: embodiment 3 makes (doctor's board match promise recovering capsule difficult to understand).
Wherein (1) fruiting body extract of Ganoderma Lucidum, Astragalus Root P.E, wolfberry fruit extract are available from the four seas, Nantong plant essence Co., Ltd (polyoses content 〉=50%); Ginkgo biloba p.e (GINKGO BILOBA EXTRACT 〉=24%); American Ginseng (total saponin content 5～10%) (2) natural VE available from DSM company (3) taurine available from Yongan, Qianjiang pharmaceutcal corporation, Ltd (99% content); (4) V
CAvailable from the stone medicine medicine company (99% content) of supporting one's family
(1) animal used as test
With age in days male and healthy Kunming mouse, body weight 20g ± 2g is purchased from the Shanghai Experimental Animal Center.
(2) experimental technique
All tested mouse are raised after 15 days under experimental situation in advance, be divided at random 4 experimental group, be control group, low dose group, middle dosage group and high dose group, 20 every group (wherein 10 are used for strengthening the immunity experiment, and 10 are used for improving anoxia endurance effect experiment).Because this compound functional preparation is 1380mg-1840mg/60kg to the day recommended amounts of human body, getting median is 1600mg/60kg, to enlarge dosage after 10 times as the middle dosage of tested mouse, be 260mg/kg, low dose group per os every day gavage amount is 130mg/kg, and high dose group per os every day gavage amount is 780mg/kg.
Experiment is respectively organized gavage liquid and is prepared (every capsules 230mg with sample distilled water, get capsule sample, remove capsule shells, the weighing content is prepared), experiment mice gavage volume is 20ml/kg, control group every day is with equivalent distilled water per os gavage, every morning gavage 1 time, gavage was tested after 30 days continuously.
(3) test method
Test method is carried out the requirement that given the test agent strengthens immunity and the raising anoxia tolerant function method of inspection with reference to " function of health food is learned assessment process and detection method ".
Test with dinitrofluorobenzene (DNFB) inducing mouse Delayed onset allergy (DTH), antibody-producting cell detects and serum hemolysin is measured, mouse spleen lymphocyte conversion test and NK cytoactive detection, antibody-producting cell detects and serum hemolysin is measured, the clearance test of mouse carbon, Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell test, the enhancing immunization effect of Comprehensive Assessment compound functional preparation sample.
With the situation of change of the indexs such as the anti-the survival time under hypoxic condition of mouse normal pressure, Ischemia Injury in Brain anaerobic respiration number of times, poisoning time-to-live of natrium nitrosum, the raising anoxia endurance action effect of Comprehensive Assessment compound functional preparation sample.
Experimental result
(1) strengthen the experimental result of immunity
1, on the impact of body weight and internal organs/body weight ratio
Before and after each dosage group mouse test, body weight gain, spleen/body ratio and thymus gland/body ratio and control group relatively, all without significant difference (P＞0.05), show that sample is not obvious to the body weight gain of mouse and internal organs/body weight ratios affect.
Table 1 various dose compound functional preparation is on the impact of mouse cell immunologic function (X ± SD)
Annotate: compared significant difference (* P＜0.05 * * P＜0.01) with control group
Table 2 various dose compound functional preparation is on the impact of mouse humoral immune functional parameter (X ± SD)
Annotate: compared significant difference (* P＜0.05 * * P＜0.01) with control group
Table 3 various dose functional preparation is on the impact of mouse monokaryon-macrophage function (X ± SD)
Annotate: compared significant difference (* P＜0.05 * * P＜0.01) with control group
Table 4 various dose compound functional preparation is on the impact of NK cells in mice activity (X ± SD)
Annotate: compared significant difference (* P＜0.05 * * P＜0.01) with control group
Per os gave the tested material of mouse various dose after 30 days, compared with control group, and the experimental result that this tested material strengthens immunity function is as follows:
Each dosage group of this compound functional preparation can strengthen the Delayed onset allergy (P＜0.05) of mouse.The carbon of mouse lymphocyte conversion capability (P＜0.05) mouse that enhancing ConA induces is cleaned up ability (P＜0.05), raising mouse antibodies cellulation number (P＜0.05), raising mouse HD50 value (P＜0.05) mouse macrophage engulfs chicken erythrocyte phagocytic rate (P＜0.05) and mouse macrophage is engulfed chicken erythrocyte phagocytic index (P＜0.05), strengthens NK cytoactive (P＜0.05) (seeing Table 1～table 4).
(2) improve the anoxia endurance experimental result
Table 5 various dose preparation is on the impact of the anti-the survival time under hypoxic condition of mouse normal pressure (X ± SD)
Annotate: compared significant difference (* P＜0.05 * * P＜0.01) with control group
Table 6 various dose preparation to mouse to Ischemia Injury in Brain anaerobic respiration times influence (X ± SD)
Annotate: compared significant difference (* P＜0.05 * * P＜0.01) with control group
Table 7 various dose preparation is on the impact of poisoning time-to-live of mouse natrium nitrosum (X ± SD)
Annotate: compared significant difference (* P＜0.05 * * P＜0.01) with control group
Per os gave the tested material of mouse various dose after 30 days, compared with control group, and the experimental result that this tested material improves anoxia tolerant function is as follows:
Basic, normal, high dosage group of the present invention can extend the anti-the survival time under hypoxic condition of normal pressure (P＜0.05) of tested mouse significantly, and the anti-anoxic effect of middle dosage group the most obviously (P＜0.01).Under the Ischemia Injury in Brain anoxia condition, this compound functional preparation can obviously reduce the brain power consumption, and cerebral hypoxia is had protective effect.On the anti-anoxia ability impact under tested mouse broken end cerebral anoxia condition significantly (P＜0.01), and the anti-anoxic effect of middle dosage group the most obviously (P＜0.01).Under the poisoning condition of natrium nitrosum, this herbal mixture functional preparation can obviously extend tested mouse natrium nitrosum poisoning (P＜0.05 of rear time-to-live, P＜0.01), improve the anti-anoxia ability of tested mouse under the poisoning condition of natrium nitrosum, and the tested mouse anti-anoxia ability of high dose group significantly (P＜0.01) (seeing Table 4～table 6).
Claims (4)
1. the health food with immunological regulation, resisting oxygen lack, is characterized in that, described health food is grouped into by the one-tenth of following weight percent proportioning:
Described health food prepares by following method:
Get fruiting body extract of Ganoderma Lucidum, Astragalus Root P.E, wolfberry fruit extract, ginkgo biloba p.e, American Ginseng (powder), taurine, natural VE, stable vitamin C; Get dolomol, mix with above-mentioned raw materials, homogeneous with colloid mill, as capsule 's content, insert in capsulae vacuus.
2. the health food with immunological regulation, resisting oxygen lack, is characterized in that, described health food is grouped into by the one-tenth of following weight proportion:
3. the health food with immunological regulation, resisting oxygen lack, is characterized in that, described health food is grouped into by the one-tenth of following weight proportion:
4. the health food with immunological regulation, resisting oxygen lack, is characterized in that, described health food is grouped into by the one-tenth of following weight proportion:
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| CN2011104197937A CN103156188A (en) | 2011-12-15 | 2011-12-15 | Health food with function of immune regulation and hypoxia tolerance |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011104197937A CN103156188A (en) | 2011-12-15 | 2011-12-15 | Health food with function of immune regulation and hypoxia tolerance |
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| CN103156188A true CN103156188A (en) | 2013-06-19 |
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| CN2011104197937A Pending CN103156188A (en) | 2011-12-15 | 2011-12-15 | Health food with function of immune regulation and hypoxia tolerance |
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| CN105341917A (en) * | 2015-11-17 | 2016-02-24 | 安徽千方生物科技有限公司 | Cell nutrition enhancing health product preparation and preparation method thereof |
| CN107495367A (en) * | 2017-08-24 | 2017-12-22 | 四川省中医药科学院 | A kind of food, health products or pharmaceutical composition for improving hypoxia-bearing capability and its production and use |
| CN114287620A (en) * | 2021-12-28 | 2022-04-08 | 云南省药物研究所 | Composition with hypoxia tolerance effect |
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Application publication date: 20130619 |