CN103110891B - Traditional Chinese medicine for treating breast hyperplasia and breast cancer - Google Patents
Traditional Chinese medicine for treating breast hyperplasia and breast cancer Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine for treating breast hyperplasia and breast cancer. The traditional Chinese medicine can be used for effectively solving the treatment medicinal problems of the breast hyperplasia and breast cancer. The technical scheme for solving the problems is as follows: the traditional Chinese medicine is prepared from radix curcumae processed with vinegar, trigone processed with vinegar, rhizoma zedoariae processed with vinegar, processed pangolin, seaweed, iphigenia indica, radix codonopsitis, astragalus root, fruit of glossy privet, honeysuckle, lithospermum, chrysanthemum, rhizoma cyper processed with vinegar, trichosanthes, rubia root, corydalis tuber processed with vinegar, uniflower swisscentaury root, rhizoma coptidis, processed vomiting nut, chilopod, rheum officinale, fried cowherb seed, fried mustard seed, tangerine seed, prepared frankincense, prepared myrrh, oarweed and paris polyphylla. The material medicines are crushed and prepared into pills by using water and dried, respectively. The material medicines are effectively combined and supported with each other to achieve the effects of detoxicating and resolving a mass, tonifying qi and dredging collaterals, and promoting blood circulation to remove blood stasis. Besides, the traditional Chinese medicine is effectively used for treating the breast hyperplasia and breast cancer, and is safe in medicine use, good in curative effect and innovative on medicines for treating the breast hyperplasia and breast cancer.
Description
Technical field
The present invention relates to Chinese medicine, particularly a kind of Chinese medicine (claiming to wither again tumor ball) for the treatment of cyclomastopathy and breast carcinoma.
Background technology
Cyclomastopathy is the modal cystic hyperplasia of breast of women, and its sickness rate accounts for the first place of mastopathy.This disease sickness rate was the trend rising year by year in the last few years, and the age also more and more becomes younger.In adolescence or young women, premenstrual have distending pain of the breast, sometimes pain can involve shoulder back, through the spontaneous remission gradually of rear udder attachment pain, only can contact mammary gland and a bit thicken, without obvious tuberosity, these are that physiological changes, not disease, do not need treatment yet, belong to physiological hypertrophy.Cyclomastopathy is normal breast lobule physiological hypertrophy and subinvolution, and mammary gland normal configuration gets muddled, and belongs to pathological proliferation, and it is neither an inflammation class disease of non-tumor again.Be mainly in 30-50 year women, onset peak is 35-40 year.
The age distribution of pathogenesis of breast carcinoma is different in country between east and west, in district occurred frequently as the country such as Northern Europe, North America, breast carcinoma was since appearance in about 20 years old, before being 45-50 year, keep the fast rise impetus in menopause, approximately 1 times of every growths 10-20 of age year sickness rate rising, after menopause, rise relatively slow, 75-85 year reaches the highest.And in low areas such as Asia, the sickness rate of breast carcinoma can slightly decline after menopause, the onset peak of general breast carcinoma is between 45-55 year, and Aisan still keeps this Characteristics of age distribution after migrating western countries.
But, no matter domestic or external, although the sickness rate of breast carcinoma is high, mortality rate constantly declines, its reason not only has benefited from women with breast cancer examination and early examines the foundation of system, more has benefited from the Protocols in Molecular Biology of development and the raising of comprehensive diagnosis and treatment standardized level in recent years.
The cause of disease is illustrated not yet completely, but many research datas show, removing outside native factor of breast carcinoma is also relevant with following factors:
1. the long-time stimulus of endogenous or exogenous estrogen: estrogenic activity plays an important role to breast carcinoma.Menstruation is risen (being less than 12 years old) or menopause evening (being later than 55 years old) too early, not fertility, and not suckling after late childbirth (the first tire is after 35 years old) or fertility, the incidence rate of breast carcinoma is higher.
2. virus: carcinogenecity RNA viruses may be relevant to breast carcinoma.
3. mammary gland atypical hyperplasia: the danger that has breast duct and lobule atypical hyperplasia person that breast carcinoma occurs increases.(noting: what say here is atypical hyperplasia, is not the cyclomastopathy in common spoken language)
4. heredity and family history: multiple also in statistics the acquisition confirmation of breast carcinoma in family.Have the women of family history of breast cancer (one-level lineal relative suffers from breast cancer), the danger that breast carcinoma occurs is 2-3 times of population.
5. trophic factor: high fat material is taken in certain dependency that has of too much and breast carcinoma.
6. lonizing radiation: accept high-level ionizing radiation, especially because making chest, other diseases accepts the women of too much radiation exposure, the danger that breast carcinoma occurs increases, and the treatment means of breast carcinoma comprises operative treatment, radiotherapy, chemotherapy, endocrine therapy and molecular targeted therapy.
Though the medicine for the treatment of cyclomastopathy, breast carcinoma and means are a lot of at present, for various reasons, its curative effect is all unsatisfactory, and therefore, the innovation on treatment cyclomastopathy, breast cancer medicines is imperative.
Summary of the invention
For above-mentioned situation, for overcoming the defect of prior art, the present invention's object is just to provide a kind of Chinese medicine for the treatment of cyclomastopathy and breast carcinoma, can effectively solve the medicine for treatment problem of cyclomastopathy and breast carcinoma.
The technical scheme that the present invention solves is that cyclomastopathy and the breast carcinoma traditional Chinese medical science are thought, belong to meridians and are obstructed, QI and blood is not lived, due to the long-pending stasis of blood of evil poison, it should be with detoxicating and resolving stagnation of pathogens, activating qi and collateral, and blood circulation promoting and blood stasis dispelling is Therapeutic Principle, accordingly, the present invention adopts following material medicine to make: vinegar Radix Curcumae (processed) 35-45g, vinegar Rhizoma Sparganii 35-45g processed, Rhizoma Curcumae (processed with vinegar) 35-45g, Squama Manis (processed) 35-45g, Sargassum 35-45g, Pseudobulbus Cremastrae Seu Pleiones 35-45g, Radix Codonopsis 35-45g, Radix Astragali 35-45g, Fructus Ligustri Lucidi 35-45g, Flos Lonicerae 35-45g, Radix Arnebiae (Radix Lithospermi) 22-26g, Flos Chrysanthemi 35-45g, Rhizoma Cyperi (processed with vinegar) 35-45g, Fructus Trichosanthis 35-45g, Radix Rubiae 35-45g, Rhizoma Corydalis (processed with vinegar) 50-70g, Radix Rhapontici 35-45g, Rhizoma Coptidis 35-45g, Semen Strychni (processed) 1-1.4g, Scolopendra 10-14g, Radix Et Rhizoma Rhei 18-22g, Semen Vaccariae (parched) 35-45g, fry Semen Sinapis 22-26g, Semen Citri Reticulatae 35-45g, Olibanum (processed) 35-45g, Myrrha (processed) 35-45g, Thallus Laminariae (Thallus Eckloniae) 35-45g and Rhizoma Paridis 35-45g, pulverize above-mentioned raw materials medicine respectively, crosses 100-120 mesh sieve, mixes, with water pill, dry, obtains (with water pill, the dry routine techniques that is),
Wherein, vinegar Radix Curcumae (processed): get Radix Curcumae sheet and mix thoroughly with rice vinegar, thoroughly stewing, put in pot and heat fried dry with slow fire, take out, cool.Every Radix Curcumae sheet 100kg, uses rice vinegar 10kg;
Vinegar Rhizoma Sparganii processed: get clean Rhizoma Sparganii sheet, photograph stir-baking with vinegar is fried to complexion changed dark, and every 100kg Rhizoma Sparganii, uses vinegar 15kg;
Rhizoma Curcumae (processed with vinegar): get clean Rhizoma Curcumae, boil to the saturating heart according to vinegar cooking method (appendix II D), take out, slightly cool, cut sheet, dry;
Squama Manis (processed): vinegar soaks all take rice vinegar as adjuvant.By boiling-on-grain, first Squama Manis sheet is concocted with oil-sand to be surface be golden yellow volume while expanding, screen out oil-sand, immerse while hot in rice vinegar (Squama Manis 100g, vinegar 30g) take out dry break into pieces for subsequent use;
Rhizoma Cyperi (processed with vinegar): will pulverize Rhizoma Cyperi and put into cylinder, and mix thoroughly with yellow wine and rice vinegar, then use Saccharum Sinensis Roxb., the appropriate melt of frying that adds water, then pours Rhizoma Cyperi in pot into, fully mixes with Saccharum Sinensis Roxb. water, fried dry, and Rhizoma Cyperi 50kg, with yellow wine, the each 10kg of rice vinegar, Saccharum Sinensis Roxb. 3kg;
Rhizoma Corydalis (processed with vinegar): get clean Rhizoma Corydalis, mix thoroughly with vinegar, infiltrate, to vinegar exhaustion, put in pot and fry to micro-and do with slow fire, take out, cool; Or get clean Rhizoma Corydalis, and put in pot and boil altogether with vinegar, to vinegar exhaustion, dry, take out, cool; (100 jin of every Rhizoma Corydalis, with 20 jin of vinegar);
Semen Strychni (processed): get sand and put in pot, after making popular with high heat, add clean Semen Strychni, constantly stir, scald to heaving and aobvious sepia or dark-brown, take out, sieve removes sand, cools;
Semen Vaccariae (parched): get clean Semen Vaccariae, fry to great majority and pop and spend in vain according to frying method (appendix II D);
Fry Semen Sinapis: the former medicine clean impurity of winnowing with a dustpan, put in pot and fry to buff, micro-savory, take out, cool;
Olibanum (processed): get the Olibanum of choosing clean put pot in slow fire fry slightly see melting point to surface, taking-up cools;
Myrrha (processed): get the Myrrha of choosing clean put pot in slow fire fry slightly see melting point to surface, taking-up cools.
Above-mentioned alleged material medicine preparation method is the conventional concocting method of pharmacopeia regulation; the core of the present invention's protection is component and the consumption of material medicine; therefore; no matter component and consumption that every employing the present invention is given, adopt the dosage form how to make and all belong to the scope of protection of the invention.
Effective combination of material medicine of the present invention, supports mutually, has effect of detoxicating and resolving stagnation of pathogens, activating qi and collateral, blood circulation promoting and blood stasis dispelling, is effective to treat cyclomastopathy and breast carcinoma, drug safety, and good effect, is the innovation on treatment cyclomastopathy and breast cancer medicines.
The specific embodiment
Below in conjunction with embodiment, the specific embodiment of the present invention is elaborated.
Embodiment 1
The present invention in the specific implementation, can adopt following material medicine to make: vinegar Radix Curcumae (processed) 40g, vinegar Rhizoma Sparganii 40g processed, Rhizoma Curcumae (processed with vinegar) 40g, Squama Manis (processed) 40g, Sargassum 40g, Pseudobulbus Cremastrae Seu Pleiones 40g, Radix Codonopsis 40g, Radix Astragali 40g, Fructus Ligustri Lucidi 40g, Flos Lonicerae 40g, Radix Arnebiae (Radix Lithospermi) 24g, Flos Chrysanthemi 40g, Rhizoma Cyperi (processed with vinegar) 40g, Fructus Trichosanthis 40g, Radix Rubiae 40g, Rhizoma Corydalis (processed with vinegar) 60g, Radix Rhapontici 40g, Rhizoma Coptidis 40g, Semen Strychni (processed) 1.2g, Scolopendra 12g, Radix Et Rhizoma Rhei 20g, Semen Vaccariae (parched) 40g, fry Semen Sinapis 24g, Semen Citri Reticulatae 40g, Olibanum (processed) 40g, Myrrha (processed) 40g, Thallus Laminariae (Thallus Eckloniae) 40g and Rhizoma Paridis 40g, above-mentioned raw materials medicine is pulverized respectively, cross 100-120 mesh sieve, mix, with water pill, dry, obtain.
Embodiment 2
The present invention in the specific implementation, also can be made by following material medicine: vinegar Radix Curcumae (processed) 35g, vinegar Rhizoma Sparganii 35g processed, Rhizoma Curcumae (processed with vinegar) 35g, Squama Manis (processed) 35g, Sargassum 35g, Pseudobulbus Cremastrae Seu Pleiones 35g, Radix Codonopsis 35g, Radix Astragali 35g, Fructus Ligustri Lucidi 35g, Flos Lonicerae 35g, Radix Arnebiae (Radix Lithospermi) 22g, Flos Chrysanthemi 35g, Rhizoma Cyperi (processed with vinegar) 35g, Fructus Trichosanthis 35g, Radix Rubiae 35g, Rhizoma Corydalis (processed with vinegar) 50g, Radix Rhapontici 35g, Rhizoma Coptidis 35g, Semen Strychni (processed) 1g, Scolopendra 10g, Radix Et Rhizoma Rhei 18g, Semen Vaccariae (parched) 35g, fry Semen Sinapis 22g, Semen Citri Reticulatae 35g, Olibanum (processed) 35g, Myrrha (processed) 35g, Thallus Laminariae (Thallus Eckloniae) 35g and Rhizoma Paridis 35g, above-mentioned raw materials medicine is pulverized respectively, cross 100-120 mesh sieve, mix, with water pill, dry, obtain.
Embodiment 3
The present invention in the specific implementation, also can be made by following material medicine: vinegar Radix Curcumae (processed) 45g, vinegar Rhizoma Sparganii 45g processed, Rhizoma Curcumae (processed with vinegar) 45g, Squama Manis (processed) 45g, Sargassum 45g, Pseudobulbus Cremastrae Seu Pleiones 45g, Radix Codonopsis 45g, Radix Astragali 45g, Fructus Ligustri Lucidi 45g, Flos Lonicerae 45g, Radix Arnebiae (Radix Lithospermi) 26g, Flos Chrysanthemi 45g, Rhizoma Cyperi (processed with vinegar) 45g, Fructus Trichosanthis 45g, Radix Rubiae 45g, Rhizoma Corydalis (processed with vinegar) 70g, Radix Rhapontici 45g, Rhizoma Coptidis 45g, Semen Strychni (processed) 1.4g, Scolopendra 14g, Radix Et Rhizoma Rhei 22g, Semen Vaccariae (parched) 45g, fry Semen Sinapis 26g, Semen Citri Reticulatae 45g, Olibanum (processed) 45g, Myrrha (processed) 45g, Thallus Laminariae (Thallus Eckloniae) 45g and Rhizoma Paridis 45g, above-mentioned raw materials medicine is pulverized respectively, cross 100-120 mesh sieve, mix, with water pill, dry, obtain.
Embodiment 4
The present invention in the specific implementation, can adopt following material medicine to make: vinegar Radix Curcumae (processed) 37g, vinegar Rhizoma Sparganii 43g processed, Rhizoma Curcumae (processed with vinegar) 37g, Squama Manis (processed) 43g, Sargassum 37g, Pseudobulbus Cremastrae Seu Pleiones 43g, Radix Codonopsis 37g, Radix Astragali 43g, Fructus Ligustri Lucidi 37g, Flos Lonicerae 43g, Radix Arnebiae (Radix Lithospermi) 23g, Flos Chrysanthemi 43g, Rhizoma Cyperi (processed with vinegar) 37g, Fructus Trichosanthis 43g, Radix Rubiae 37g, Rhizoma Corydalis (processed with vinegar) 78g, Radix Rhapontici 37g, Rhizoma Coptidis 43g, Semen Strychni (processed) 1.1g, Scolopendra 13g, Radix Et Rhizoma Rhei 19g, Semen Vaccariae (parched) 43g, fry Semen Sinapis 23g, Semen Citri Reticulatae 43g, Olibanum (processed) 37g, Myrrha (processed) 43g, Thallus Laminariae (Thallus Eckloniae) 37g and Rhizoma Paridis 43g, above-mentioned raw materials medicine is pulverized respectively, cross 100-120 mesh sieve, mix, with water pill, dry, obtain.
Embodiment 5
The present invention in the specific implementation, can adopt following material medicine to make: vinegar Radix Curcumae (processed) 43g, vinegar Rhizoma Sparganii 37g processed, Rhizoma Curcumae (processed with vinegar) 43g, Squama Manis (processed) 37g, Sargassum 43g, Pseudobulbus Cremastrae Seu Pleiones 37g, Radix Codonopsis 43g, Radix Astragali 37g, Fructus Ligustri Lucidi 43g, Flos Lonicerae 37g, Radix Arnebiae (Radix Lithospermi) 25g, Flos Chrysanthemi 37g, Rhizoma Cyperi (processed with vinegar) 43g, Fructus Trichosanthis 37g, Radix Rubiae 43g, Rhizoma Corydalis (processed with vinegar) 55g, Radix Rhapontici 43g, Rhizoma Coptidis 37g, Semen Strychni (processed) 1.3g, Scolopendra 11g, Radix Et Rhizoma Rhei 21g, Semen Vaccariae (parched) 37g, fry Semen Sinapis 23g, Semen Citri Reticulatae 37g, Olibanum (processed) 43g, Myrrha (processed) 37g, Thallus Laminariae (Thallus Eckloniae) 43g and Rhizoma Paridis 37g, above-mentioned raw materials medicine is pulverized respectively, cross 100-120 mesh sieve, mix, with water pill, dry, obtain.
Effective combination of said medicine, supports mutually, has detoxicating and resolving stagnation of pathogens, activating qi and collateral, and effect of blood circulation promoting and blood stasis dispelling, is effective to cyclomastopathy and breast carcinoma, and has obtained satisfied useful technique effect through clinical experiment, and interrelated data is as follows:
One, select the standard of case
A, cyclomastopathy clinical symptoms show as:
1, mastalgia: be often distending pain or twinge, can involve one or both sides breast, heavy common with a lateral deviation, pain severe patient can not be touched, and even affects daily life and work.Pain can be to homonymy axillary fossa or the radiation of shoulder back; Part can show as sore nipples or itch.Premenstrual a couple of days of mastalgia Chang Yuyue occurs or increases the weight of, and after passing through, pain obviously alleviates or disappears; Pain also can fluctuate with emotion changes, fatigue, Changes in weather.This pain relevant with menstrual cycle and emotion changes is the main feature of cyclomastopathy clinical manifestation;
2, lump in breast: lump can be sent out in one-sided or bilateral breast, single or multiple, is generally apt to occur in the outside upper quadrant of breast.Show as lamellar not of uniform size, nodositas, streak etc., wherein take lamellar as common.Border is not obvious, and quality is medium or slightly hard, without adhesion, often has tenderness with surrounding tissue.Most of lump in breast also has the feature changing with menstrual cycle, and a month premenstrual lump increases hardening, and after menstrual onset, lump dwindles deliquescing;
3, nipple discharge: nipple discharge can appear in small number of patients, are spontaneous discharge, mostly are faint yellow or light oyster white, also have few person to overflow as seen discharge through extruding nipple.It is careful to need if there is courageous and upright or coffee-like discharge;
By the person that has above-mentioned symptom, all as the standard of selecting case, and give Chinese medicine pill treatment of the present invention;
B, breast carcinoma clinical symptoms show as:
1. lump in breast: be the modal performance of breast carcinoma.
2. nipple changes: nipple discharge mostly are optimum change, but to more than 50 years old, has one-sided nipple discharge person should watch out for the probability that breast carcinoma occurs; Crater nipple; It is the clinical manifestation of mammary gland Paget (Paget disease) that the eczema samples such as nipple pruritus, desquamation, erosion, ulcer, incrustation often change.
3. skin of breast and profile change: the Cooper ligament of tumor invading skin, can form " dimple sign "; Tumor cell stops up subcutaneous lymphatic capillary, causes hydroderma, and hair follicle place is recessed to form " Pericarpium Citri tangerinae is levied "; In the time that skin is extensively invaded, can form most hard lesser tubercle or little rope at epidermis, even merge in flakes, as pathological changes extends to back and offside thoracic wall can limit breathing, form corset cancer; Inflammatory breast cancer there will be breast obviously to increase, and dermathemia redness, local skin temperature increase; In addition, advanced breast cancer there will be skin ulceration to form cancer ulcer.
4. lymphadenectasis: homonymy axillary gland can enlargement, and advanced breast cancer can cause enlargement to offside axillary lymphatic metastasis; Also can contact in some cases in addition homonymy and or offside clavicle on enlarged lymph node.
By the person that has above-mentioned symptom, all as the standard of selecting case, and give Chinese medicine pill treatment of the present invention.
Two, diagnostic criteria
Cyclomastopathy: the symptom that all standard A with above-mentioned selection case provide, with reference to " traditional Chinese medical science disease Standardization of diagnosis and curative effect " (ZY/T0012-94, within 1994, State Administration of Traditional Chinese Medicine issues), make a definite diagnosis through ultrasound diagnosis, mammography, the inspection of mammary gland nuclear-magnetism;
Breast carcinoma: the symptom that all standard B with above-mentioned selection case provide, with reference to " traditional Chinese medical science disease Standardization of diagnosis and curative effect " (ZY/T0012-94, within 1994, State Administration of Traditional Chinese Medicine issues), make a definite diagnosis through ultrasound diagnosis, mammography, the inspection of mammary gland nuclear-magnetism.
Three, therapeutic scheme
Cyclomastopathy: through suffering from cyclomastopathy person and give Chinese medicine pill of the present invention treatment making a definite diagnosis, oral, 3 times on the one, each 6g, 3-6 month statistics curative effect.
Breast carcinoma: through suffering from breast carcinoma person and give Chinese medicine pill of the present invention treatment making a definite diagnosis, oral, 3 times on the one, each 6g, adds up curative effect for 3-5.
Four, efficacy assessment standard
Cyclomastopathy
Healing: with reference to " traditional Chinese medical science disease Standardization of diagnosis and curative effect " (ZY/T0012-94, State Administration of Traditional Chinese Medicine issued in 1994), mastalgia, lump disappear completely through treatment, nothing recurrence in 5 years;
Effective: mastalgia disappears or occasionally has mild pain, lump to dwindle and is greater than 1/2;
Effective: mastalgia alleviates to some extent, lump dwindles and is less than 1/2;
Invalid: mastalgia, lump are through treating without significant change, even there is the trend increasing the weight of.
Breast carcinoma
Effective: through treatment, mastalgia, lump disappear through treatment, and life span extends more than 5 years;
Effective: through treatment, symptom is improved, life span extends 3 months to 3 years;
Invalid: through treatment, symptom is without any improvement, even there is the trend increasing the weight of.
Five, statistical procedures
A, from 1998 so far between 15 years, applicant is through being in hospital and outpatient service, applying Chinese medicine pill of the present invention treats by such scheme 47000 people, curative effect is subject to extensive favorable comment, wherein, and cyclomastopathy 35000 people, effective percentage can reach more than 97%, mammary cancer 1 2000 people, the effective percentage of the auxiliary treatment of postoperative chemotherapy can reach more than 91%, and specifically treatment situation is added up as following table:
| ? | Number | Cure (%) | Effective (%) | Effectively (%) | Invalid (%) | Total effective rate (%) |
| Cyclomastopathy | 35000 | 66.5 | 17.3 | 13.4 | 2.8 | 97.2 |
| Breast carcinoma | 12000 | 0 | 68.5 | 23.0 | 8.5 | 91.5 |
B, Chinese medicine pill of the present invention other different doctors beyond applicant try out, and have all obtained identical with applicant and akin experimental result, and statistical conditions are as follows:
Certain hospital (1) curing mammary gland hyperplasia situation
Certain hospital (2) curing mammary gland hyperplasia situation
The order of prompting remission is: soften → conscious pain disappearance → body of gland agglomerate disappearance → body of gland tenderness of alleviate → agglomerate of mastalgia disappears completely.Before and after treatment, detect serum E2, P, PRL, measure prompting E2, PRL and reduce, P raises, and Epidemiological Analysis by statistics, has significant difference (P<005).
Applicant, in clinical practice, is also provided with matched group especially, carries out randomized controlled treatment with Chinese medicine pill of the present invention, result shows, Chinese medicine pill of the present invention is obviously better than matched group, and matched group adopts chemotherapeutic treatment, treatment group adopts chemotherapy and Chinese medicine pill combined treatment of the present invention, and relevant situation is as follows:
Before treatment, patient's CD3, CD4 and CD4/CD8 ratio, all lower than Normal group, illustrate that the cellular immune function of Postoperative Patient with Breast Cancer is in relatively low state.After chemotherapy, Cellular immunity suppression obviously increases the weight of, and after the Chinese medicine pill treatment of the present invention for the treatment of group use in conjunction, CD3, CD4 and CD4/CD8 ratio, all higher than matched group, illustrate that coupling Chinese medicine pill of the present invention (treatment group) is to keeping immunity to have positive effect.Statistical table is as follows:
Treatment group of the present invention is compared with matched group, and ★ P<0.05, has comparability, and treatment group is obviously better than matched group.
Chinese medicine pill of the present invention (claiming to wither again tumor ball) also proves to have breast cancer tumour cells apoptosis through zoopery, and relevant experimental data is as follows:
1 experiment material
1. 48 of 1 material SD rats, body weight (250 ± 20) g, female, by providing from Medical School of Zhengzhou University zoopery center.Sub-cage rearing, freely ingests, intakes, and Mus room keeps ventilating.Human breast carcinoma cell lines MCF-7, is provided by Beijing Military Medical Science Institute Cell Lab.
1. the main medicine of 2 medicines and main agents: Chinese medicine pill of the present invention (tumor of withering ball), this pill is ground into powder, add by distilled water and make in 0. 5% carboxymethylcellulose sodium solution, be mixed with concentration and be 30% suspendible medicinal liquid.Fluorouracil Injection ［ the accurate word H31020593 of traditional Chinese medicines ］ 10mL:0. 25g is produced by Shanghai Xudong Hipu Medicine Co., Ltd.Physiological saline solution is 3mgmol by the dilution of 5-fu injection
-1, 4 ℃ of Refrigerator stores.Decoction of four noble drugs requires to make the about 200mL of decoction according to Chinese medicinal formulae.Normal saline ［ the accurate word H37022097 of traditional Chinese medicines ］ 100mL is produced by the healthy and free from worry pharmaceutcal corporation, Ltd in Shandong.
Main agents: DMEM culture medium dry powder (Gibco, the U.S.) hyclone (Gibco, the U.S.) trypsin Gibco, the U.S.) RNase A enzyme (Sigma, the U.S.) the domestic analytical pure of PI (Sigma, the U.S.) other reagent.
2 experimental techniques
2. the preparing reference literature method ［ 1-2 ］ and improve points 4 groups at random of 48 mices of 1 mice Contained Serum and cell culture medium, every group gives corresponding Chinese medicine preparation 2mL gavage (0. 1gL having prepared
-1kg
-1d
-1), being equivalent to 25 times of adult amount, blank serum group gives isodose normal saline gavage, the lumbar injection next day of the every daily medicinal liquid having diluted of 5-fu group, each (0. 15gL
-1kg
-12d
-1), continuous 7 days of above processing.After last perfusion (before perfusion, water 12h is can't help in fasting) and lumbar injection 1h, 3% pentobarbital (1. 5gL-1) intraperitoneal anesthesia, ventral aorta blood sampling, 3000r/min
-1× 20min centrifugalize serum, 56 ℃ of water-bath 30min deactivation ,-70 ℃ of freezing preservations.Minimal medium: DMEM lyophilized powder is imported in beaker, add NaHCO32. 8g, HEPES 2. 3g, 900mL tri-distilled water, magnetic bar stirs, and adjusts pH value to 7. 2～7. 4, is settled to 1000mL, filter filtration sterilization subpackage, 4 ℃ of preservations.It is 10% that hyclone is diluted to final concentration with DMEM culture fluid, is mixed with 10% hyclone cell culture fluid.Each group of serum use of deactivation, DMEM culture fluid is diluted to four groups of Contained Serums that final concentration is 10% concentration (tumor of withering ball group, decoction of four noble drugs group, 5-Fu group, normal saline group) respectively.2. 2 cell culture recovery human breast cancer cell strain MCF-7, are seeded in the DMEM culture fluid containing 10% hyclone, penicillin 100 μ g/mL, are placed in 37 ℃, 5% CO
2aseptic culture in incubator, cell is adherent growth, with trypsinization, within 2～3 days, goes down to posterity 1 time.Exponential phase, adjusting cell concentration is 1 × 10
5individual/mL, is inoculated in two 6 well culture plates, and every hole adds 2mL cell suspension, is placed in 37 ℃, 5% CO
2in incubator, cultivate 24h.Change corresponding culture fluid, two culture plates are made as parallel-plate.Add respectively four kinds of Contained Serums, cultivate 72h, collecting cell.2. 3 cells were tested by flow cytometry apoptosis rate reference literatures are also improved ［ 3-4 ］, the take the logarithm human breast cancer cell of trophophase, the take the logarithm human breast cancer cell of trophophase, uses trypsinization collecting cell, and 1000r/min × 5min is centrifugal, abandoning supernatant, PBS is centrifugal to be washed once, and 70% 4 ℃ of ethanol fixedly spend the night, 37 ℃ of digestion 40min of RNA enzyme, 4 ℃ of dyeing 1h of iodate the third ingot, every duplicate samples measures 1 × 10
5individual cell, carries out upper machine testing with FACS flow cytometer (BD company of the U.S.); Every duplicate samples measures 1 × 10
4individual cell, carries out cell cycle analysis, and the DNA content of mensuration shows with rectangular histogram, and the percentage ratio in each cycle of cell is determined in the machine processing as calculated of histogrammic data.The hypodiploid peak that is positioned at the DNA content minimizing of G0-G1 phase is " apoptotic peak " (being apoptotic cell), and its shared ratio is apoptosis rate.2, be respectively blank serum group, decoction of four noble drugs group, 5-fu group and the tumor ball group of withering.Every 2. 4 statistical method statistical procedures all adopt SPSS 13. 0 statistical softwares, mean ± standard deviation for statistical data (
± s) represent row one factor analysis of variance (one-way ANOVA), mean difference between more each group of multiple comparisons between employing group (LSD method, Dunnett T3).
3 experimental results
MCF-7 cell after Contained Serum intervention detects the phenomena of apoptosis finding that there is in various degree through flow cytometer, cell cycle and apoptosis rate see the following form.
The impact of Contained Serum on human breast cancer cell MCF-7 cell cycle (%, 72h,
± s)
| Group | G0～G1 phase | G2～M phase | The S phase | Apo (apoptosis XISHUAI) |
| The tumor of withering ball group | 43.72 ±0.51 | 24.08 ±0.29 | 32.32 ±0.29＊＊ | 17.28 ± 0.34 |
| Decoction of four noble drugs group | 45.63 ±1.47 | 19.01 ±1.70 | 35.10 ±0.72* | 11.63 ± 0.75 |
| 5-FU group | 50.96 ± 1.92＊＊ | 20.43 ± 2.83 | 28.60 ± 1.32 | 18.88 ± 2.95 |
| Normal saline group | 46.15 ±1.1 | 14.67 ±1.53 | 39.17 ±1.39＊＊ | 1.13 ± 0.25 |
Organize shared cell percentage ratio apparently higher than other groups at G0/G1 phase 5-Fu, the tumor of withering ball group and all the other two groups of difference are little, the tumor of withering ball group and 5-Fu comparing difference be (P < 0. 01) significantly, and 5-Fu group is also remarkable (P < 0. 01) compared with other two groups of differences.Wither tumor ball group cell percentage apparently higher than other groups in the G2/M phase, with decoction of four noble drugs group, relatively zero difference (P > 0. 05) of 5-Fu group, relatively there were significant differences (P < 0. 01) with normal saline group.Be less than other two groups at S phase 5-Fu group, the tumor of withering ball group percentage; relatively show difference (P < 0. 05) with these two groups, 5-Fu group relatively has notable difference (P < 0. 01) with other two groups; The tumor of withering ball group and decoction of four noble drugs be zero difference (P > 0. 05) relatively, relatively has significant difference (P < 0. 01) with normal saline group.The tumor of withering ball and 5-FU group apoptotic peak are obvious; Wherein, be 18. 1% containing the apoptosis rate of the tumor ball serum that withers, apparently higher than 0. 86% of blank group; Each group of apoptosis rate carried out to variance analysis and show heterogeneity of variance (F=5. 71 P=0. 012), between organizing with DunnettT3, comparative analysis shows: relatively there were significant differences (P < 0. 01) with decoction of four noble drugs group; Compare without zero difference (P > 0. 05) with 5-Fu group; Relatively there were significant differences (P < 0. 01) with normal saline group.Result shows containing the tumor ball rat blood serum that withers to have the apoptotic effect of induction MCF-7.
Apoptosis of tumor cells effect.PI staining adds the ratio that scattered light conjoint analysis can quantitative analysis apoptotic cell, and great advantage is to carry out cell cycle analysis simultaneously.Carry out flow cytometer after to human breast carcinoma MCF-7 cytosis 72h by Contained Serum and detect the experiment of cell cycle and apoptosis; found that MCF-7 cell is after the effect 72h containing the tumor ball drug serum that withers; containing withering, tumor ball Contained Serum can block MCF-7 cell cycle in the G2/M phase; it is the Leukopenia that enters the S phase; the copying of DNA, synthesize, mitosis reduces, and suppresses MCF-7 cell proliferation by this mechanism; According to apoptosis testing result, show to there is the apoptotic effect of induction MCF-7 containing the rat blood serum of the tumor ball that withers; And can tentatively infer that the tumor ball that withers is relevant with cell death inducing to the inhibitory action mechanism of MCF-7 cell, but concrete mechanism needs further to study, and as to gene induced apoptosis such as P53, Bcl-2 families, and affects generation and the development of breast carcinoma.
Can clearly be found out by above-mentioned experiment, the present invention induces human breast carcinoma cell lines MCF-7 apoptosis, can suppress MCF-7 cell proliferation containing Chinese medicine pill serum of the present invention; Serum can block the MCF-7 cell cycle G2/M phase, and the S phase, shared cell proportion declined, and can induce MCF-7 apoptosis, benefited the treatment to breast carcinoma.
Six, conclusion
Shown by above-mentioned data, Chinese medicine pill of the present invention has detoxicating and resolving stagnation of pathogens, activating qi and collateral, effect of blood circulation promoting and blood stasis dispelling, can be effective to treat cyclomastopathy, and breast cancer cell is had to apoptosis and inhibitory action, be beneficial to the treatment to breast carcinoma, taking convenience, good effect, to treating cyclomastopathy effective percentage up to 97%, treatment breast carcinoma effective percentage is reached to 91%, and the particularly medicine for treatment after mammary cancer chemotherapy, is obviously better than simple chemotherapy to the treatment after mammary cancer chemotherapy, be the active drug for the treatment of cyclomastopathy and breast carcinoma, there is significant clinical meaning.
Claims (5)
1. treat the Chinese medicine of cyclomastopathy and breast carcinoma for one kind, it is characterized in that, made by following material medicine: vinegar Radix Curcumae (processed) 35-45g, vinegar Rhizoma Sparganii 35-45g processed, Rhizoma Curcumae (processed with vinegar) 35-45g, Squama Manis (processed) 35-45g, Sargassum 35-45g, Pseudobulbus Cremastrae Seu Pleiones 35-45g, Radix Codonopsis 35-45g, Radix Astragali 35-45g, Fructus Ligustri Lucidi 35-45g, Flos Lonicerae 35-45g, Radix Arnebiae (Radix Lithospermi) 22-26g, Flos Chrysanthemi 35-45g, Rhizoma Cyperi (processed with vinegar) 35-45g, Fructus Trichosanthis 35-45g, Radix Rubiae 35-45g, Rhizoma Corydalis (processed with vinegar) 50-70g, Radix Rhapontici 35-45g, Rhizoma Coptidis 35-45g, Semen Strychni (processed) 1-1.4g, Scolopendra 10-14g, Radix Et Rhizoma Rhei 18-22g, Semen Vaccariae (parched) 35-45g, fry Semen Sinapis 22-26g, Semen Citri Reticulatae 35-45g, Olibanum (processed) 35-45g, Myrrha (processed) 35-45g, Thallus Laminariae (Thallus Eckloniae) 35-45g and Rhizoma Paridis 35-45g, above-mentioned raw materials medicine is pulverized respectively, cross 100-120 mesh sieve, mix, with water pill, dry, obtain.
2. the Chinese medicine for the treatment of cyclomastopathy according to claim 1 and breast carcinoma, it is characterized in that, made by following material medicine: vinegar Radix Curcumae (processed) 40g, vinegar Rhizoma Sparganii 40g processed, Rhizoma Curcumae (processed with vinegar) 40g, Squama Manis (processed) 40g, Sargassum 40g, Pseudobulbus Cremastrae Seu Pleiones 40g, Radix Codonopsis 40g, Radix Astragali 40g, Fructus Ligustri Lucidi 40g, Flos Lonicerae 40g, Radix Arnebiae (Radix Lithospermi) 24g, Flos Chrysanthemi 40g, Rhizoma Cyperi (processed with vinegar) 40g, Fructus Trichosanthis 40g, Radix Rubiae 40g, Rhizoma Corydalis (processed with vinegar) 60g, Radix Rhapontici 40g, Rhizoma Coptidis 40g, Semen Strychni (processed) 1.2g, Scolopendra 12g, Radix Et Rhizoma Rhei 20g, Semen Vaccariae (parched) 40g, fry Semen Sinapis 24g, Semen Citri Reticulatae 40g, Olibanum (processed) 40g, Myrrha (processed) 40g, Thallus Laminariae (Thallus Eckloniae) 40g and Rhizoma Paridis 40g, above-mentioned raw materials medicine is pulverized respectively, cross 100-120 mesh sieve, mix, with water pill, dry, obtain.
3. the Chinese medicine for the treatment of cyclomastopathy according to claim 1 and breast carcinoma, it is characterized in that, made by following material medicine: vinegar Radix Curcumae (processed) 35g, vinegar Rhizoma Sparganii 35g processed, Rhizoma Curcumae (processed with vinegar) 35g, Squama Manis (processed) 35g, Sargassum 35g, Pseudobulbus Cremastrae Seu Pleiones 35g, Radix Codonopsis 35g, Radix Astragali 35g, Fructus Ligustri Lucidi 35g, Flos Lonicerae 35g, Radix Arnebiae (Radix Lithospermi) 22g, Flos Chrysanthemi 35g, Rhizoma Cyperi (processed with vinegar) 35g, Fructus Trichosanthis 35g, Radix Rubiae 35g, Rhizoma Corydalis (processed with vinegar) 50g, Radix Rhapontici 35g, Rhizoma Coptidis 35g, Semen Strychni (processed) 1g, Scolopendra 10g, Radix Et Rhizoma Rhei 18g, Semen Vaccariae (parched) 35g, fry Semen Sinapis 22g, Semen Citri Reticulatae 35g, Olibanum (processed) 35g, Myrrha (processed) 35g, Thallus Laminariae (Thallus Eckloniae) 35g and Rhizoma Paridis 35g, above-mentioned raw materials medicine is pulverized respectively, cross 100-120 mesh sieve, mix, with water pill, dry, obtain.
4. the Chinese medicine for the treatment of cyclomastopathy according to claim 1 and breast carcinoma, it is characterized in that, made by following material medicine: vinegar Radix Curcumae (processed) 45g, vinegar Rhizoma Sparganii 45g processed, Rhizoma Curcumae (processed with vinegar) 45g, Squama Manis (processed) 45g, Sargassum 45g, Pseudobulbus Cremastrae Seu Pleiones 45g, Radix Codonopsis 45g, Radix Astragali 45g, Fructus Ligustri Lucidi 45g, Flos Lonicerae 45g, Radix Arnebiae (Radix Lithospermi) 26g, Flos Chrysanthemi 45g, Rhizoma Cyperi (processed with vinegar) 45g, Fructus Trichosanthis 45g, Radix Rubiae 45g, Rhizoma Corydalis (processed with vinegar) 70g, Radix Rhapontici 45g, Rhizoma Coptidis 45g, Semen Strychni (processed) 1.4g, Scolopendra 14g, Radix Et Rhizoma Rhei 22g, Semen Vaccariae (parched) 45g, fry Semen Sinapis 26g, Semen Citri Reticulatae 45g, Olibanum (processed) 45g, Myrrha (processed) 45g, Thallus Laminariae (Thallus Eckloniae) 45g and Rhizoma Paridis 45g, above-mentioned raw materials medicine is pulverized respectively, cross 100-120 mesh sieve, mix, with water pill, dry, obtain.
5. the Chinese medicine for the treatment of cyclomastopathy according to claim 1 and breast carcinoma, it is characterized in that, made by following material medicine: vinegar Radix Curcumae (processed) 43g, vinegar Rhizoma Sparganii 37g processed, Rhizoma Curcumae (processed with vinegar) 43g, Squama Manis (processed) 37g, Sargassum 43g, Pseudobulbus Cremastrae Seu Pleiones 37g, Radix Codonopsis 43g, Radix Astragali 37g, Fructus Ligustri Lucidi 43g, Flos Lonicerae 37g, Radix Arnebiae (Radix Lithospermi) 25g, Flos Chrysanthemi 37g, Rhizoma Cyperi (processed with vinegar) 43g, Fructus Trichosanthis 37g, Radix Rubiae 43g, Rhizoma Corydalis (processed with vinegar) 55g, Radix Rhapontici 43g, Rhizoma Coptidis 37g, Semen Strychni (processed) 1.3g, Scolopendra 11g, Radix Et Rhizoma Rhei 21g, Semen Vaccariae (parched) 37g, fry Semen Sinapis 23g, Semen Citri Reticulatae 37g, Olibanum (processed) 43g, Myrrha (processed) 37g, Thallus Laminariae (Thallus Eckloniae) 43g and Rhizoma Paridis 37g, above-mentioned raw materials medicine is pulverized respectively, cross 100-120 mesh sieve, mix, with water pill, dry, obtain.
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