CN103109866A - Light-efficiency accelerant for non-leaf organs of plants and preparation method thereof - Google Patents

Light-efficiency accelerant for non-leaf organs of plants and preparation method thereof Download PDF

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CN103109866A
CN103109866A CN2013100365222A CN201310036522A CN103109866A CN 103109866 A CN103109866 A CN 103109866A CN 2013100365222 A CN2013100365222 A CN 2013100365222A CN 201310036522 A CN201310036522 A CN 201310036522A CN 103109866 A CN103109866 A CN 103109866A
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liquid
plants
light efficiency
foliage organ
foliage
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CN103109866B (en
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李俊
张春雷
马霓
李玲
廖星
袁金展
余常兵
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Oil Crops Research Institute of Chinese Academy of Agriculture Sciences
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Oil Crops Research Institute of Chinese Academy of Agriculture Sciences
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Abstract

The invention discloses a light-efficiency accelerant for non-leaf organs of plants and a preparation method thereof. The light-efficiency accelerant is prepared from nanometer titanium dioxide, titanium dioxide nanotube, low sodium polyacrylate, potassium bicarbonate, dormin, brassinolide, potassium phosphate, sodium dodecyl sulfate, ethanol and sodium hydroxide according to proportions. The preparation method comprises the steps of: A. firstly dissolving the nanometer titanium dioxide and titanium dioxide nanotube in the ethanol, adding distilled water, carrying out ultrasonic concussion, adding low sodium polyacrylate, controlling under an aright constant temperature condition, using NaOH for adjusting pH, and preparing liquid A; B. dissolving potassium bicarbonate, dormin, brassinolide and potassium phosphate in the distilled water, and adjusting pH to obtain liquid B; and C. pouring the liquid A into the liquid B, stirring while pouring, adding sodium dodecyl sulfate to the mixture of the liquid A and the liquid B, placing at a constant temperature, and carrying out magnetic stirring to obtain the stable nanoscale small-bubble solution. The light-efficiency accelerant is reasonable in formula, convenient to use, free of environmental pollution and convenient to popularize and apply and has a direct effect and high utilization rate of nutrition.

Description

The one non-foliage organ light efficiency of Plants promoter and preparation method thereof
Technical field
The invention belongs to bioengineering field, more specifically relate to the non-foliage organ light efficiency of a Plants promoter, also relate to simultaneously the preparation method of the non-foliage organ light efficiency of a Plants promoter.This promoter is suitable for each breeding time of the various crop such as grain, cotton, oil, melon and fruit, flowers, promotes the increasing both production and income of these crops.
Background technology
Non-foliage organ means except blade, and all organs that contains chloroplast are as parts such as stem stalk, fruit crust, fruit ear axles.As everyone knows, photosynthesis is the physiological foundation that crop yield forms, and the power of utility rate of luminous energy has determined the height of crop yield.Yet the current overwhelming majority's research all concentrates on the photosynthesis that how to improve plant leaf blade, also studies very less for the photosynthesis of the non-foliage organ of plant, and particularly Related product does not almost have especially.But more and more studies show that, the non-foliage organ photosynthesis of plant is very huge to the contribution of output, as wheatear section, stem stalk, fruit crust and lepicena, the impact of output is reached 3564%, the non-foliage organ photosynthesis such as rape stem, angle pericarp affect contribution rate 70% left and right to output.Find by the research to non-foliage organ photosynthetic pathway, they all have the blade of being different from C 3Or C 4The feature of metabolic pathway, its photosynthetic rate is apparently higher than blade.Because non-foliage organ such as stem stalk, petiole etc. have the organic matter such as screen casing transportation system, can efficiently shift its photosynthesis assimilation product in " storehouse ".Therefore, improve non-foliage organ photosynthesis and may cause the leap of crop yield and the green revolution of a new generation.The development of China's light efficiency promoter at present also is in the starting stage, not only light efficiency promoter kind seldom, and most of light efficiency promoter all is based on the transience light efficiency promotion of leaf nutrition and increase blade carbon source, output and economic benefit action effect to plant can not be permanent, also need to spray repeatedly simultaneously just can play certain effect.The present invention passes through the first step: the photosynthesis enzyme of the non-foliage organ of regulation and control activation plant, second step: nutrition and the carbon source of supplying with the non-foliage organ necessity of plant; The 3rd step: at a kind of nanometer photosynthetic membrane of non-foliage organ surface formation, improve on the one hand the surface temperature of the non-foliage organ of rape, improve on the other hand non-foliage organ surface light scattering strength, and then improve the photosynthetic capacity of non-foliage organ.
Summary of the invention
The objective of the invention is to be to provide the non-foliage organ light efficiency of a Plants promoter, formula rationally, and is easy to use, can improve to greatest extent the photosynthetic efficiency of non-foliage organ.Simultaneously, because non-foliage organ has the characteristics such as photosynthetic rate is high, large to the output contribution, the photosynthetic product transhipment is rapid, therefore with respect to blade light efficiency promoter, non-foliage organ light efficiency promoter has the advantages such as effect is direct, obvious effect of increasing production, economic coefficient is high, effect is lasting.
Another object of the present invention is the preparation method who has been to provide the non-foliage organ light efficiency of a Plants promoter, and methodological science is easily gone, and is easy and simple to handle; The composition stably dispersing, nutrient utilization is high; Non-environmental-pollution, easy to utilize.
In order to realize above-mentioned purpose, the present invention adopts following technical measures:
The one non-foliage organ light efficiency of Plants promoter, it is made by the mass fraction of following raw material:
Figure BDA00002798227500021
The one non-foliage organ light efficiency of Plants promoter, it makes (preferable range) by the mass fraction of following raw material:
Figure BDA00002798227500022
The one non-foliage organ light efficiency of Plants promoter, it makes (better scope) by the mass fraction of following raw material:
Figure BDA00002798227500031
The one non-foliage organ light efficiency of Plants promoter, it makes (good scope) by the mass fraction of following raw material:
Figure BDA00002798227500032
The one non-foliage organ light efficiency of Plants promoter, it makes (optimum range) by the mass fraction of following raw material:
Figure BDA00002798227500041
The one non-foliage organ light efficiency of Plants promoter, it makes (optimum value) by the mass fraction of following raw material:
Figure BDA00002798227500042
The preparation method of the one non-foliage organ light efficiency of Plants promoter, at first dissolve the nano titanium oxide of 0.20.4 mass fraction with 95% ethanol of 12 mass fractions, 0.010.05 titania nanotube and the sodium low polyacrylate 8.515.0 mass fraction of part mass fraction, add the distilled water of 2030 mass fractions, after ultrasonic oscillation 1822min, airtight constant temperature control after 2024 hours, transfers pH value to 8.0 to make A liquid with NaOH standby; Take the saleratus of 1.09.5 part quality, 0.010.2 the dormin of part quality, 0.150.85 the study on brassinolide of mass fraction with the distilled water dissolving of 3040 mass fractions, is made B liquid with the potassium phosphate adjustment pH value to 8.0 of 510 mass fractions standby.The 3rd step slowly poured A liquid in B liquid, and the limit bevelling stirs, and the sodium dodecyl that takes again 35 mass fractions after A liquid and B liquid are completely integrated adds wherein, is placed into and stirs 2024min on constant temperature blender with magnetic force.Namely make non-foliage organ photosynthesis promoter mother liquor after the dissolving fully.Application concentration 5001500ppm.Spray the time: seedling stage, elongation, florescence and fruiting period.
The steps include:
A, first with 95% ethanol dissolving nano titanium oxide and titania nanotube, add ultrasonic concussion 1822min after 300ml distilled water, add sodium low polyacrylate, airtight constant temperature control 2224 hours, NaOH transfers pH value to 7.8-8.2 and is settled to 500ml, makes A liquid;
B, saleratus, dormin, study on brassinolide, potassium phosphate etc. are settled to 500ml with the rear accent of distilled water dissolving pH value to 7.88.2 and make B liquid;
C, then A liquid is slowly poured in B liquid, the limit bevelling stirs, and A liquid and B liquid mix add sodium dodecyl fully, are placed into and stir 2024min on constant temperature blender with magnetic force.Obtain overstable nanoscale vesicle solution.
From 2010, the applicant successively patrols base, military empty farm, Qianjiang, Aug. 1st farm, Qichun at Inst. of Oil Crops, Chinese Academy of Agriculture's sun, nearly more than 50 point time tests are completed altogether when sun, From Zhongxiang Hubei, Suizhou, hubei, Nanchang, Yichuan, liuyang hunan and other places in Yaan, Sichuan, Wuhu, Chaohu, Anhui Province, China, Hubei, no matter professional research institution or farm, peasant household, participate in the experimenter and all unanimously think, the present invention has the following advantages:
1. effect of increasing production is good.Product of the present invention adopts the aspect systematicness such as astigmatism, nutrition, carbon source, regulation and control to strengthen photosynthesis of plant according to the international newest research results of current plant photosynthesis, and photosynthetic efficiency improves more than 1.5 times than general light efficiency promoter; While can effectively guarantee the abundance of plant organic matter and supply with fast, improves flowering of plant fruit-setting rate and Grain Filling degree, thus remarkable high yield.
Through 2011 ~ 2012 years two test years, successively exist, the crop of participating in the experiment comprises more than totally two ten kinds such as grain, economic crops, vegetables, test, demonstration area accumulative total reach more than 500 mu.The unit of participating in the experiment has carried out overall merit to 2011 ~ 2012 years test yield result:
(1) the comprehensive average amount of increase in production of cereal crops: soybean 2011 annual volume increase 37.6%; 2012 annual volume increase 44.8%; Paddy rice 2011 annual volume increase 18.1%; 2012 annual volume increase 19.6%; Corn 2011 annual volume increase 27.7%; 2012 annual volume increase 31.4%.
(2) economic crops: rape on average increases production 45%; Sesame on average increases production 40%; Beet on average increases production 32.1%; White melon on average increases production 43.1%; Stevia on average increases production 18.2%.
(3) 15 kinds of comprehensive average amounts of increase in production 17% ~ 68% such as vegetables, leaf vegetables, fruit and vegetable, tubers.
Two 11 as example in the rape variety of oil crop research institute of middle Academy of Agricultural Sciences seed selection: the data that after the 2012 non-foliage organ photosynthesis promoters of annual seedling spraying, elongation is measured as: spray after non-foliage organ photosynthesis promoter leaf area index and contrast and increase by 66.70%; Photosynthetic gesture contrasts increases by 39.96%; Net Photosynthetic Rate contrasts increases by 81.41%; Processing when chlorophyll total amount: 1:500 doubly sprays increases by 38.22%, 1:800 than contrast and doubly sprays to process and increase by 24.13%, 1:1000 than contrast and doubly spray to process than contrast and increase by 14.82%.
2. significantly improve fruit quality.Product of the present invention both provided crop needed balanced nutrient to regulate the Crop Source base relation as the basis, can promote again flower bud differentiation of crop and bloom, and strengthened pollination; Promote fruit to form, alleviate physiological and non-physiologic shedding, and improve the sugar content of fruit; We show in the result of the test of changde, hunan, after spraying this product, compared with the control, significantly improve quality of tobacco: cigarette strain leaf area significantly increases, tobacco leaf chemical composition total reducing sugar (28.66%), reducing sugar (22.71%), potassium content (2.76%), two sugar all reach sound tobacco standard index (with reference to " People's Republic of China (PRC) tobacco business standard, 2002 ") than (0.98) and sugared alkali than (9.08) etc. than (5.01), nitrogen base than (0.89), potassium chlorine.Simultaneously 2011-2012 we the result of the test of grape and pears is shown, spray this product and can promote Fruit, significantly improve single fruit weight and output, promote mouthfeel, fruit type, fruit look, the glossy degree of fruit etc.
3, to having and regulate metabolic physiological function for studying thing, concrete manifestation make obviously strengthen for the resistance that studies thing as: drought resistance, disease resistance; Promote root growth point growth rate in the wound restore funcitons etc.Under drought condition, the result of the test on wheat, soybean (1:500 ~ 1000 times) is shown, spray that processing contrasts effective tiller, branch increases 1 ~ 3, leaf area increases more than 20%, can effectively control simultaneously the fruit drop under adverse environmental factor, promote fringe grain, pod grain to increase by 35% ~ 60%, thereby effectively reduce adverse circumstance to the impact of crop yield.
4. applicability is wide.Product of the present invention can be applied to grain, economic crops, vegetables etc. and more than totally two ten plant crop, all can use at seedling stage, elongation, the florescence of crop.Productive phase, also can be widely used in non-plant or the crop that eats or the protection of pod skin is arranged such as cotton, rape, soybean.
5. the succinct science of filling a prescription, material source is wide, cost is low.Compound basis crop photosynthesis principle of the present invention, through great many of experiments and optimization of C/C composites, formula is succinct and effect is remarkable; Raw materials all can be purchased acquisition on market, the cost of raw material is low, the source is wide.
6. have that anti-hard water does not flocculate, the particular advantages such as chemistry and biologically active height, be specially adapted to used as pesticides, chemical fertilizer potentiating and produce high-efficiency foliage fertilizer, growth regulator, soil conditioner, drought resistance agent, bio-fertilizer active carrier and other agricultural spraying dispenser agent etc.
Embodiment
Embodiment 1:
The one non-foliage organ light efficiency of Plants promoter, comprise nano titanium oxide (P25) 0.4 gram, titania nanotube 0.04 gram, sodium low polyacrylate 12.0g, saleratus 7.5 grams, dormin 0.02 gram, study on brassinolide 0.5 gram, potassium phosphate 1.0 grams, sodium dodecyl 3.0g, 95% ethanol 2.0 grams, sodium hydroxide 0.05 gram, surplus is distilled water 73.49 grams; Namely made by the mass fraction of following raw material:
Figure BDA00002798227500071
Concrete preparation process is:
A, first with 95% ethanol dissolving nano titanium oxide and titania nanotube, add after 300ml distilled water ultrasonic concussion 18 or 19 or 20 or 21 or 22min, add sodium low polyacrylate, airtight constant temperature control 20 or 21 or 22 or 23 or 24 hours, NaOH transfers pH value to 7.8 or 7.9 or 8 or 8.1 or 8.2 and be settled to 500ml, makes A liquid;
B, saleratus, dormin, study on brassinolide, potassium phosphate etc. are settled to 500ml with the rear accent of distilled water dissolving pH value to 7.8 or 7.9 or 8 or 8.1 or 8.2 and make B liquid;
C, then A liquid is slowly poured in B liquid, the limit bevelling stirs, and A liquid and B liquid mix add sodium dodecyl fully, is placed into to stir 20 or 21 or 22 or 23 or 24min on constant temperature blender with magnetic force.Obtain overstable nanoscale vesicle solution.
Get this preparation 20ml seedling stage at sesame, add and evenly spray at noon on sesame stem and petiole after 10 liters, clear water (diluting 500 times) mixes, measure the petiole photosynthetic rate after 7 days, and measure output after results.The photosynthesis effect is with to compare result as follows:
Figure BDA00002798227500081
Annotate: utility rate of luminous energy=Net Photosynthetic Rate/intensity of illumination; Efficiency of water application=Net Photosynthetic Rate/transpiration rate.Lower same
Embodiment 2:
The one non-foliage organ light efficiency of Plants promoter, comprise nano titanium oxide (P25) 0.35 gram, titania nanotube 0.06 gram, sodium low polyacrylate 10.0g, saleratus 8.0 grams, dormin 0.05 gram, study on brassinolide 0.60 gram, potassium phosphate 0.90 gram, sodium dodecyl 3.5g, 95% ethanol 1.5 grams, sodium hydroxide 0.05 gram, surplus is distilled water 74.99 grams.Be that the material quality mark is:
Figure BDA00002798227500082
Preparation process is identical with embodiment 1.
Get this preparation 20ml at the soybean florescence, add and evenly spray at noon on soybean pod pericarp, stem and petiole after 10 liters, clear water (diluting 500 times) mixes, the knot angle phase is measured soybean pod pericarp photosynthetic rate, and measures output after results.The photosynthesis effect is with to compare result as follows:
Figure BDA00002798227500091
Embodiment 3:
The one non-foliage organ light efficiency of Plants promoter, comprise nano titanium oxide (P25) 0.3 gram, titania nanotube 0.05 gram, sodium low polyacrylate 12.0g, saleratus 9.0 grams, dormin 0.1 gram, study on brassinolide 0.5 gram, potassium phosphate 1.2 grams, sodium dodecyl 3.5g, 95% ethanol 1.8 grams, sodium hydroxide 0.05 gram, surplus distilled water are 71.5 grams.Namely made by following material quality mark:
Figure BDA00002798227500092
Preparation process is identical with embodiment 1.
Get this preparation 20ml at the rape initial bloom stage, add and evenly spray at noon on siliqua of oilseed rape skin, stem, branch after 16 liters, clear water (diluting 800 times) mixes, the knot angle phase is measured siliqua of oilseed rape skin photosynthetic rate, and measures output after results.The photosynthesis effect is with to compare result as follows:
Figure BDA00002798227500101
Embodiment 4:
The one non-foliage organ light efficiency of Plants promoter, comprise nano titanium oxide (P25) 0.3 gram, titania nanotube 0.03 gram, sodium low polyacrylate 12.5g, saleratus 7.0 grams, dormin 0.12 gram, study on brassinolide 0.6 gram, potassium phosphate 1.25 grams, sodium dodecyl 4.2g, 95% ethanol 1.4 grams, sodium hydroxide 0.05 gram, surplus distilled water 72.55 grams.It is made by the mass fraction of following raw material:
Preparation process is identical with embodiment 1.
Get this preparation 20ml at the cotton flowering and boll-setting period, add and evenly spray at noon on cotton bell, stem, branch after 20 liters, clear water (diluting 1000 times) mixes, measure the stem photosynthetic rate after 7 days, and measure the rear output of results.The photosynthesis effect is with to compare result as follows:
Figure BDA00002798227500103
Figure BDA00002798227500111
Use preparation of the present invention and carry out spraying test at seedling stage and the florescence of the crops such as rape, soybean, peanut, paddy rice, wheat and sesame respectively, the equal highly significant of effect of increasing production is particularly at the effect highly significant at florescence.
Seedling stage effect
Crop Application method Result of the test
Rape Seedling spraying is diluted 800 times of liquid Angle fruit number increases by 33%, and output increases by 42%
Soybean Seedling spraying is diluted 500 times of liquid The pod number increases by 41%, and output increases by 49%
Peanut Seedling spraying is diluted 500 times of liquid The pod number increases by 20%, and output increases by 32%
Cotton Seedling spraying is diluted 800 times of liquid Peach bell number increases by 19%, 31%
Sesame Seedling spraying is diluted 500 times of liquid The capsule number increases by 24, and output increases by 41%
The florescence effect
Crop Application method Result of the test
Rape Spraying in the flowering stage dilutes 800 times of liquid Output increases by 56%
Soybean Spraying in the flowering stage dilutes 1000 times of liquid Output increases by 68%
Peanut Spraying in the flowering stage dilutes 800 times of liquid Output increases by 44%
Cotton Spraying in the flowering stage dilutes 800 times of liquid Output increases by 62%
Sesame Spraying in the flowering stage dilutes 1000 times of liquid Output increases by 39%

Claims (7)

1. the non-foliage organ light efficiency of a Plants promoter, it is made by the mass fraction of following raw material:
Figure FDA00002798227400011
2. a Plants non-foliage organ light efficiency promoter described according to right 1 is characterized in that:
Figure FDA00002798227400012
3. a Plants non-foliage organ light efficiency promoter described according to right 1 is characterized in that:
Figure FDA00002798227400013
Figure FDA00002798227400021
4. a Plants non-foliage organ light efficiency promoter described according to right 1 is characterized in that:
5. a Plants non-foliage organ light efficiency promoter described according to right 1 is characterized in that:
Figure FDA00002798227400023
Figure FDA00002798227400031
6. a Plants non-foliage organ light efficiency promoter described according to right 1 is characterized in that:
Figure FDA00002798227400032
7. the preparation method of the non-foliage organ light efficiency of a Plants promoter claimed in claim 1, the steps include:
A, first with 95% ethanol dissolving nano titanium oxide and titania nanotube, add ultrasonic concussion 18-22min after 300ml distilled water, add sodium low polyacrylate, airtight constant temperature control 22-24 hour, NaOH transfers pH value to 7.8-8.2 and is settled to 500ml, makes A liquid;
B, saleratus, dormin, study on brassinolide, potassium phosphate are settled to 500ml with the rear accent of distilled water dissolving pH value to 7.8-8.2 and make B liquid;
C, then A liquid is slowly poured in B liquid, the limit bevelling stirs, and A liquid and B liquid mix add sodium dodecyl fully, are placed into and stir 20-24min on constant temperature blender with magnetic force.Obtain overstable nanoscale vesicle solution.
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CN107306994A (en) * 2017-07-17 2017-11-03 陈菁 Nanometer plant photosynthesis promoter and its preparation method and application
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CN107306994A (en) * 2017-07-17 2017-11-03 陈菁 Nanometer plant photosynthesis promoter and its preparation method and application
CN110771625A (en) * 2019-09-17 2020-02-11 江苏新南洋绿色科技有限公司 Wettable agent formula for enhancing high-temperature stress resistance of crops
CN114467730A (en) * 2022-02-25 2022-05-13 安徽农业大学 Method for increasing contents of nicotinamide and trehalose of non-heading Chinese cabbage
CN114467730B (en) * 2022-02-25 2022-11-15 安徽农业大学 Method for increasing contents of nicotinamide and trehalose of non-heading Chinese cabbage

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