CN103082091B - Special nutrition strengthening solution for livestock and poultry and preparation method thereof - Google Patents
Special nutrition strengthening solution for livestock and poultry and preparation method thereof Download PDFInfo
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- CN103082091B CN103082091B CN201310015520.5A CN201310015520A CN103082091B CN 103082091 B CN103082091 B CN 103082091B CN 201310015520 A CN201310015520 A CN 201310015520A CN 103082091 B CN103082091 B CN 103082091B
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Abstract
The invention relates to a special nutrition strengthening solution for livestock and poultry and a preparation method thereof, belonging to the technical field of functional nutritional products. The special nutrition strengthening solution for the livestock and the poultry, provided by the invention, comprises the following components: 3%-12% of vitamins, 1%-5% of amino acids, 5%-30% of emulsifier, 2%-5% of co-emulsifier, 0.1%-0.5% of antimicrobial preservative and the balance of purified water. The specific preparation method comprises the following steps: firstly adding the emulsifier, the co-emulsifier and the purified water, stirring, then adding the vitamins, the amino acids and the antimicrobial preservative, dissolving and stirring for 10-30 minutes to get the special nutrition strengthening solution for the livestock and the poultry. The special nutrition strengthening solution for the livestock and the poultry, prepared by the preparation method provided by the invention, can improve water solubility, dispersion and absorptivity of the vitamins, thoroughly solve the problem of bioavailability of the fat-soluble vitamins and provide the nutrition strengthening solution which can be highly absorbed and utilized for the livestock and the poultry with liver and gallbladder diseases.
Description
Technical field
The invention belongs to functional nutrient product technical field, relate to a kind of livestock and poultry special purpose nutrition-strengthening liquid and preparation method thereof.
Background technology
Vitamin is that a class formation is different, the necessary small organic molecule of normal activities.Different from three major nutrient, the energy that vitamin produces in metabolic process is very little, neither the part of body tissue structure, and mainly forms coenzyme or the prothetic group of enzyme, participates in the metabolism of Auto-regulator and energy.The function that each vitamin has it special to animal body, animal lacks any one vitamin all can cause specific Nutrition and Metabolism obstacle, occurs vitamin-deficiency, the lighter can cause grow be obstructed, production capacity declines, and even causes death.
Current market finding vitamin mostly is solid pharmaceutical preparation, Powdered or graininess, in use dissolves not exclusively, easily blocks water fountain, and breed bacteria, be not suitable for Animal Populations drinking water administration; In addition, fat soluble vitamin absorption in vitamin solid pharmaceutical preparation and fat accompany, the surface that could arrive absorptive cell,intestinal through the liquid environment in enteric cavity must be dissolved via the bile of hepatic secretion, have the livestock and poultry of hepatobiliary disease can cause the malabsorption of liposoluble vitamin.
Nanometer technology has just been born phase later 1980s and the new technology emerged, and is one of three large cutting edge technologies of world today's development in science and technology.The application of nanometer technology in field of medicaments pharmacy, creates administration nano-drug administration system (Nanoparticle drug delivery system, NDDS).
Administration nano-drug administration system comprises the delivery systems such as nanoparticle, nanosphere, nanocapsule, nano liposomes, nano-emulsion.Nano-emulsion (nanoemulsion) wherein, also micro emulsion (microemulsion) is claimed, that a particle diameter be made up of oil, water, surfactant, cosurfactant Four composition is 10 ~ 100nm, drop mostly is spherical, size is even, and transparent or semitransparent, tool Thermodynamically stable and isotropism, pressure sterilizing or high speed centrifugation stablize the not stratified and dispersion system of colloid that preparation condition is gentle, formula suitably can be formed voluntarily.Its advantage has: it is isotropic transparency liquid, Thermodynamically stable, can filtration sterilization, is easy to preserve; By the oil phase of kernel and the two-part solubilising of the hydrocarbon chain of surfactant, the solubility of medicine greatly can be improved; Physical stability is good, can improve the stability of medicine, easily prepare; Viscosity is low, can not cause pain during injection, can not cause allergy and fat embolism; Surface tension is low, and easily by the hydrated sheath of gastrointestinal wall, medicine can directly be contacted with gastrointestinal epithelial cell, nano-emulsion particle diameter is little and evenly, the good dispersion of medicine, can increase the permeability of film, thus promotes the absorption of medicine, can improve the bioavilability etc. of medicine; Medicine for facile hydrolysis is made water-in-oil type nanoemulsion and can be played a protective role; Also can extend the drug release time of water soluble drug; Medicine can be protected to a certain extent in intestines and stomach to exempt from enzymolysis, avoid the first pass effect of liver, stomach and intestine; Intravenously administrable has certain tissue, organ targeting; Also can change the distribution in vivo of some drugs, have to a certain extent and reduce the toxic and side effect of medicine at some tissue, organ, the allergic reaction of medicine can be reduced; Clinical application extensively, by oral, injection, rectum, through administrations such as skins; Also has the advantage such as certain slowly-releasing or targeting.
Summary of the invention
The object of the invention is to utilize nano-emulsion technology, a kind of livestock and poultry special purpose nutrition-strengthening liquid is provided, improve the water-soluble, dispersed of vitamin and absorptivity, thorough solution liposoluble vitamin bioavailability concerns, for hepatobiliary disease livestock and poultry provide a kind of can high absorption utilize fortification liquid.
Another object of the present invention is to provide the preparation method of livestock and poultry special purpose nutrition-strengthening liquid, and this preparation method is simple, it is low to consume energy, production cost is low.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
A kind of livestock and poultry special purpose nutrition-strengthening liquid, by weight percentage, comprises following component: vitamin 3% ~ 12%; Amino acid/11 % ~ 5%; Emulsifying agent 5% ~ 30%; Assistant for emulsifying agent 2% ~ 5%; Bacteriostatic preservative 0.1% ~ 0.5%; All the other are purified water.
Preferably, described vitamin comprises liposoluble vitamin and water soluble vitamin.
Further, described liposoluble vitamin comprises: vitamin A 0.5% ~ 2%, vitamin D
30.1% ~ 0.5% and vitamin E 0.2% ~ 1%.
Further, described water soluble vitamin comprises: Cobastab
10.1% ~ 0.4%, Cobastab
20.05% ~ 0.4%, Cobastab
60.1% ~ 0.4%, Cobastab
120.005% ~ 0.02%, vitamin C 0.5% ~ 3%, vitamin K
30.02% ~ 0.2%, niacinamide 0.5% ~ 3%, calcium pantothenate 0.5% ~ 2%, inositol 0.5% ~ 2% and biotin 0.001% ~ 0.05%.
Preferably, described amino acid comprises methionine 0.1% ~ 0.2%, lysine 0.2% ~ 1%, threonine 0.1% ~ 0.5%, arginine 0.1% ~ 0.5% and taurine 0.5% ~ 3%.
Preferably, described emulsifying agent is one or more in lecithin, soybean lecithin, fatty glyceride, polyoxyethylene sorbitan fatty acid ester, Span series sorbitan fatty acid ester, Myrij series polyoxyethylene fatty acid ester, Brij series polyoxyethylene aliphatic alcohol ether, EL series castor oil polyoxyethylene ether and poloxamer.
Preferably, described assistant for emulsifying agent is one or more in ethanol, propane diols, glycerine, ethylene glycol, n-butanol and polyethylene glycol.
Preferably, described bacteriostatic preservative is one or more in p-hydroxybenzoate, benzyl alcohol, methaform, Phenoxyethanol, phenol, quaternary ammonium compound, benzoic acid and sorbic acid.
A kind of preparation method of livestock and poultry special purpose nutrition-strengthening liquid, concrete operation step is: first add emulsifying agent, assistant for emulsifying agent and purified water and stir, add vitamin, amino acid, bacteriostatic preservative dissolving subsequently, stir 10min ~ 30min, obtain described livestock and poultry special purpose nutrition-strengthening liquid.
Compared with prior art, the present invention has following beneficial effect:
1, the livestock and poultry special purpose nutrition-strengthening liquid security prepared of the present invention is good;
2, the livestock and poultry special purpose nutrition-strengthening liquid that prepared by the present invention is oil-in-water type nano-emulsion, for isotropic thermodynamic stable system, significantly improve the dissolubility of vitamin in water, dispersiveness and dialysis performance, liposoluble vitamin can be made in stomach and intestine to discharge rapidly, good contact is had with intestines and stomach upper strata mucous layer, by GI epithelium mucomembranous cell interstitial, enter blood circulation, thus change vitamin physiological and biochemical procedure in vivo, and eliminate the difference absorbed between individuality, improve bioavilability;
3, preparation method proposed by the invention is simple, do not need high-pressure homogeneous, heat, cool contour energy consumption process, production cost is low, is applicable to suitability for industrialized production.
Detailed description of the invention
Example of the present invention can not limit the present invention to explanation of the present invention, and any change in the implication suitable with the present invention and scope and adjustment, all should think within the scope of the invention.
Below in conjunction with embodiment, the present invention will be further described.
Embodiment 1
A preparation method for livestock and poultry special purpose nutrition-strengthening liquid, specifically comprises the following steps:
1, measuring appropriate purified water is placed in container, accurately takes emulsifying agent, assistant for emulsifying agent adds in container, slowly stir;
2, in container, water soluble vitamin, amino acid, bacteriostatic preservative is added, slow stirring and dissolving;
3, liposoluble vitamin is added container, slowly stir 10 ~ 30min, form transparent supernatant liquid;
4, add water to 1000ml, stir evenly the livestock and poultry special purpose nutrition-strengthening liquid that gets product, be nano-emulsion.
In this preparation method step, conventional emulsifying agent has lecithin, soybean lecithin, fatty glyceride, polyoxyethylene sorbitan fatty acid ester, Span series sorbitan fatty acid ester, Myrij series polyoxyethylene fatty acid ester, Brij series polyoxyethylene aliphatic alcohol ether, EL series castor oil polyoxyethylene ether and poloxamer.Wherein: lecithin belongs to a kind of mixture, it is the one group of filemot oil material be present among animal vegetable tissue and yolk, its constituent comprises phosphoric acid, choline, aliphatic acid, glycerine, glycolipid, triglyceride and phosphatide, and lecithin is described as " the 3rd nutrient " arranged side by side with protein, vitamin; Soybean lecithin is a kind of mixed phosphatide, it is by phosphatid ylcholine (lecithin, be called for short PC, high-grade is PPC), phosphatidyl-ethanolamine (cephalin, be called for short PE), phosphatidylinositols (lipositol is called for short PI), phosphatidyl serine (silk amino acid phosphatide, be called for short PS) etc. become be grouped into, phosphatide is the basis of human body cell (cell membrane, nuclear membrane, plastid film), and has important relationship to merits such as nerve, reproduction, hormones, has very high nutritive value and medical value.
Polyoxyethylene sorbitan fatty acid ester, be called for short polysorbate (Polysorbate), also emulsifying agent Tween is, for sapn (Span, Span) and the condensation product of oxirane, be a class non-ionic detergent, it comprises a variety of, such as: Tween-60 (Tween-60) is stearate; Tween-80 (Tween-80) is oleate; Tween-20 (Tween-20) is laurate, is the mixture of the two Span 20 of Tween 20 and a part of polyoxyethylene.Preferred emulsifier is Tween-80 in the present invention.
Sorbitan fatty acid ester (Span series) is EPE polyol EPE, chemically structure is seen, this kind of surfactant is the partial fatty acid ester of polyalcohol, it belongs to non-ionic surface active agent, a kind of excellent Water-In-Oil or oil-in-water type surfactant, wherein the emulsibility of Span-80 has high-quality most, and preferred emulsifier is Span-80 in the present invention.
Myrijj series polyoxyethylene fatty acid ester has comparatively strong water-soluble, and emulsifying capacity is strong, and it comprises Myrij-45, Myrij-49, Myrij-51, Myrij-52 and Myrij-53.
EL series castor oil polyoxyethylene ether has comparatively strong water-soluble, comprise EL-20, EL-40 and EL-80, wherein EL-40 is oil-in-water emulsifiers, crude oil is made in woolen industry, there is good antistatic behaviour, better with other antistatic additive blending results of use, this product is also made emulsifying agent for industries such as ink, pharmacy, agricultural chemicals and is used; EL-80 can make the component of the preparation mediums such as acrylic fibers, polyvinyl, polypropylene fibre, has antistatic behaviour and emulsification, can make the emulsifying agent of organophosphorus pesticide Rogor, emulsion polymerisation emulsifying agent, the emulsifying agent of crude oil, is widely used in the industries such as textile printing and dyeing, coating, ink, is also used as the emulsifying agent of various oil product.
Poloxamer (Poloxamer) is polyoxyethylene poly-oxygen propylene aether block copolymer, commodity are called general stream Buddhist nun gram (Pluronic), it is the novel macromolecule non-ionic surface active agent of a class, at present for one of vein emulsion only a few synthetic emulsifier as a kind of oil-water emulsifiers, the emulsion prepared with it, newborn grain is few, general below 1 μm, absorptivity is high, and physical property is stablized, and preferred emulsifier is poloxamer in the present invention.
Preferred emulsifier is one or more in Tween80, span80, soybean lecithin and poloxamer in the present invention, and in the present embodiment, preferred emulsifier is Tween80, and its percentage by weight is 5%.
Conventional assistant for emulsifying agent has ethanol, propane diols, glycerine, ethylene glycol, n-butanol and polyethylene glycol, wherein polyethylene glycol by oxirane and water or ethylene glycol progressively addition polymerization form, the polyethylene glycol that relative molecular weight is lower can be used as solvent, cosolvent, O/W type emulsifying agent and stabilizing agent, assistant for emulsifying agent can be PEG400, propane diols comprises 1, 2-propane diols and 1, ammediol, 1, 2-propane diols can as the assistant for emulsifying agent of high-quality, assistant for emulsifying agent preferably 1 in the present invention, 2-propane diols, one or more in glycerine and PEG400, in the present embodiment, preferred assistant for emulsifying agent is PEG400, its percentage by weight is 2%.
Vitamin is humans and animals nutrition, grows some a small amount of organic compound necessary, effect of the utmost importance is had to the metabolism of body, growth, growth, health, if certain vitamin of long-term lacking, will cause physiological function obstacle and certain disease occurs, therefore vitamin is the necessary component of livestock and poultry special purpose nutrition-strengthening liquid.Vitamin comprises liposoluble vitamin and water soluble vitamin, and wherein water soluble vitamin comprises Cobastab
10.1% ~ 0.4%, Cobastab
20.05% ~ 0.4%, Cobastab
60.1% ~ 0.4%, Cobastab
120.005% ~ 0.02%, vitamin C 0.5% ~ 3%, vitamin K
30.02% ~ 0.2%, niacinamide 0.5% ~ 3%, calcium pantothenate 0.5% ~ 2%, inositol 0.5% ~ 2% and biotin 0.001% ~ 0.05%; Liposoluble vitamin comprises vitamin A 0.5% ~ 2%, vitamin D
30.1% ~ 0.5% and vitamin E 0.2% ~ 1%, vitamin A and vitamin D
3specification is all selected to be 1,000,000 IU/g in the present invention; In the present embodiment, each weight percentages of components of preferred liposoluble vitamin is: vitamin A 0.5%, vitamin D
30.1%, vitamin E 0.2%, the mass percent of water soluble vitamin is respectively: Cobastab
10.1%, Cobastab
20.05%, Cobastab
60.1%, Cobastab
120.005%, vitamin C 0.5%, vitamin K
30.02%, niacinamide 0.5%, calcium pantothenate 0.5%, inositol 0.5% and biotin 0.001%.
Amino acid (amino acid) refers to the common name of the class organic compound containing amino and carboxyl, it is the basic composition unit of biological function macro-molecular protein, being the base substance forming Animal nutrition desired protein, is the organic compound containing a basic amine group and an acidic carboxypolymer.Conventional amino acid comprises methionine, threonine, lysine, arginine and taurine, wherein the effect of lysine promotes brain development, is the constituent of liver and courage, can promotes fat metabolism, regulate pineal body, mammary gland, corpus luteum and ovary, prevent cell degradation; Methionine participates in composition hemoglobin, tissue and serum, has the function promoting spleen, pancreas and lymph; Threonine some amino acid that changes reaches the function of balance; Arginine can increase the activity of arginine in liver (arginase), contributes to changing the ammonia in blood into urea and excreting out, and institute is to hyperammonemia, and hepatic dysfunction etc. are all powerful; Taurine is mainly from RE, and kidney regulates its discharge rate according to content of taurine in meals, to maintain the relatively stable of content of taurine in body.
In the present invention, amino acid comprises methionine 0.1% ~ 0.2%, lysine 0.2% ~ 1%, threonine 0.1% ~ 0.5%, arginine 0.1% ~ 0.5% and taurine 0.5% ~ 3%; In the present embodiment, the percentage by weight of each component of preferred amino acid is: methionine 0.1%, lysine 0.2%, threonine 0.1%, arginine 0.1% and taurine 0.5%.
Conventional bacteriostatic preservative has p-hydroxybenzoate, benzyl alcohol, methaform, Phenoxyethanol, phenol and its derivatives, quaternary ammonium compound, benzoic acid, sorbic acid or their basic salt, the basic salt potassium sorbate of the preferred benzoic basic salt Sodium Benzoate of bacteriostatic preservative or sorbic acid in the present invention, bacteriostatic preservative preferred sodium benzoate in the present embodiment, its percentage by weight is 0.1%.
Embodiment 2
1, measuring 700mL purified water is placed in container, selects that emulsifying agent Tween80 mass percent is 15%, the mass percent of PEG400 is 4% add in container, slowly stir;
2, in container, add water soluble vitamin, (each constituent mass percentage of water soluble vitamin is Cobastab
10.1%, Cobastab
20.05%, Cobastab
60.1%, Cobastab
120.005%, vitamin C 0.5%, vitamin K
30.02%, niacinamide 0.5%, calcium pantothenate 0.5%, inositol 0.5% and biotin 0.001%), amino acid (each constituent mass percentage of amino acid is methionine 0.1%, lysine 0.2%, threonine 0.1%, arginine 0.1% and taurine 0.5%), Sodium Benzoate 0.3%, slow stirring and dissolving;
3, by liposoluble vitamin, (each constituent mass percentage of liposoluble vitamin is vitamin A (1,000,000 IU/g) 0.5%, vitamin D
3(1,000,000 IU/g) 0.1% and vitamin E 0.2%) add container, slowly stir 10 ~ 30min, form transparent supernatant liquid;
4, add water to 1000mL, stir evenly the nano-emulsion that gets product.
Embodiment 3
Identical with embodiment 2, difference is:
Tween80 mass percent is 30%, the mass percent of PEG400 is 5%; Cobastab
10.4%, Cobastab
20.4%, Cobastab
60.4%, Cobastab
120.02%, vitamin C 3%, vitamin K
30.2%, niacinamide 3%, calcium pantothenate 2%, inositol 2%, biotin 0.05%; Dehydroretinol %, vitamin D
30.5%, vitamin e1 %; Methionine 0.2%, lysine 1%, threonine 0.5%, arginine 0.5%, taurine 3%; Potassium sorbate 0.3%.
Embodiment 4
Identical with embodiment 2, difference is:
Tween80 mass percent is 20%, the mass percent of PEG400 is 4%; Cobastab
10.3%, Cobastab
20.2%, Cobastab
60.3%, Cobastab
120.01%, vitamin C 1.5%, vitamin K
30.1%, niacinamide 1.5%, calcium pantothenate 1.2%, inositol 1.2%, biotin 0.025%; Retinol1 .2%, vitamin D
30.3%, vitamin E 0.6%; Methionine 0.15%, lysine 0.6%, threonine 0.3%, arginine 0.3%, taurine 1.7%; Potassium sorbate 0.3%.
Embodiment 5
Identical with embodiment 4, difference is:
Tween80 mass percent is 15%, Span80 mass percent is 5%, the mass percent of 1,2-PD is 4%; Cobastab
10.3%, Cobastab
20.2%, Cobastab
60.3%, Cobastab
120.01%, vitamin C 1.5%, vitamin K
30.1%, niacinamide 1.5%, calcium pantothenate 1.2%, inositol 1.2%, biotin 0.025%; Retinol1 .2%, vitamin D
30.3%, vitamin E 0.6%; Methionine 0.15%, lysine 0.6%, threonine 0.3%, arginine 0.3%, taurine 1.7%; Potassium sorbate 0.3%.
Embodiment 6
Identical with embodiment 4, difference is:
Soybean lecithin mass percent is 10%, poloxamer mass percent is 10%, and the mass percent of 1,2-PD is 4%; Cobastab
10.3%, Cobastab
20.2%, Cobastab
60.3%, Cobastab
120.01%, vitamin C 1.5%, vitamin K
30.1%, niacinamide 1.5%, calcium pantothenate 1.2%, inositol 1.2%, biotin 0.025%; Retinol1 .2%, vitamin D
30.3%, vitamin E 0.6%; Methionine 0.15%, lysine 0.6%, threonine 0.3%, arginine 0.3%, taurine 1.7%; Sodium Benzoate 0.3%.
Embodiment 7
Identical with embodiment 4, difference is:
Soybean lecithin mass percent is 10%, poloxamer mass percent is 10%, and the mass percent of glycerine is 4%; Cobastab
10.3%, Cobastab
20.2%, Cobastab
60.3%, Cobastab
120.01%, vitamin C 1.5%, vitamin K
30.1%, niacinamide 1.5%, calcium pantothenate 1.2%, inositol 1.2%, biotin 0.025%; Retinol1 .2%, vitamin D
30.3%, vitamin E 0.6%; Methionine 0.15%, lysine 0.6%, threonine 0.3%, arginine 0.3%, taurine 1.7%; Potassium sorbate 0.3%.
Embodiment 8
One, nano-emulsion type is differentiated
Identified the type of this nano-emulsion by decoration method, decoration method be utilize oil-soluble dyes Sudan red III and water-soluble dye serge blue red or blue in nano-emulsion diffusion speed to judge the type of nano-emulsion.
Result: in the obtained nano-emulsion of embodiment 1 ~ 7, blue diffusion velocity is greater than red diffusion velocity, proves that this system is O/W type nano-emulsion.
Two, microscopic pattern is observed and particle size determination
The microscopic pattern of nano-emulsion is detected with transmission electron microscope, copper mesh is placed on stencil plate, copper mesh drips a droplet nano-emulsion, naturally dry, 2% phosphotungstic acid is dropped on stencil plate, the copper mesh dried is inverted on dye liquor, negative staining 15min, dry rear upper electron microscopic observation again, the form of nano-emulsion drop can be seen, particle size and distribution.According to the engineer's scale that electromicroscopic photograph indicates, carry out nano-emulsion drop diameter measurement and go forward side by side line number according to statistics, calculate mean particle size.
Result: the emulsion droplet of the nano-emulsion preparation that embodiment 1 ~ 7 is obtained is ball-shaped, and size is even, and particle size distribution range is narrow, and granularity accounts for 90% at the particle of 20 ~ 30nm, and the average grain diameter of nano-emulsion is about 25.0 ± 2.0nm, and particle diameter is more even.
Three, stability test
Keep 100 times of acceleration of gravity centrifugation 5min as nano-emulsion and be not separated, can think stable.
Result: nano-emulsion is put into supercentrifuge, with 10000rpm centrifugation 20min, the system of embodiment 1 ~ 7 is still the homogeneous liquid of faint yellow clear, judges that nano-emulsion is as stabilising system.
Embodiment 9
Growth performance comparative test
The livestock and poultry special purpose nutrition-strengthening liquid obtained to embodiment 1 ~ 7 does growth performance test, and by obtained for embodiment 1 ~ 7 fortification liquid numbering 1#, 2#, 3#, 4#, 5#, 6#, 7# respectively, active constituent content is identical; 700 7 aa broiler chicken, are divided into seven groups immediately, often organize 100, raise in cages, free choice feeding and drinking-water, routine immunization; Fortification liquid respectively 100mL is watered 100L, is used in conjunction 5d, uses once week about, until experiment terminates; During 35 age in days, weigh on an empty stomach, statistics feed intake, calculates average daily gain, average daily ingestion amount, feedstuff-meat ratio, and carries out statistical analysis to seven groups of growth performances.Duration of test respectively organizes pig growth performance basic condition in table 1, as shown in table 1, wherein in table 1, and average daily gain (g/d)=(average weight-average initial weight) (g)/28(d); Feedstuff-meat ratio=average daily ingestion amount (g/d)/average daily gain (g/d).
Table 1
| Project | 1# | 2# | 3# | 4# | 5# | 6# | 7# |
| Average initial weight (g) | 102.8 | 103.5 | 103.8 | 104.3 | 103.5 | 103.8 | 104.5 |
| Average weight (g) | 1580.5 | 1620.8 | 1630.8 | 1780.4 | 1590.2 | 1625.3 | 1650.6 |
| Average daily gain (g/d) | 52.8 | 54.2 | 54.5 | 59.9 | 53.1 | 54.3 | 55.2 |
| Average daily ingestion amount (g/d) | 111.6 | 115.7 | 115.5 | 125.6 | 113.1 | 115.0 | 118.5 |
| Feedstuff-meat ratio | 2.12 | 2.14 | 2.12 | 2.10 | 2.13 | 2.12 | 2.15 |
As can be seen from Table 1, the average daily ingestion amount of 1# ~ 7#, average daily gain, 35 age in days body weight all have remarkable increase, feedstuff-meat ratio is more or less the same, but the average daily ingestion amount of 4#, average daily gain, 35 age in days body weight are in embodiment the heaviest, feedstuff-meat ratio is minimum in embodiment, therefore comparatively speaking, embodiment 4 is most preferred embodiment.
Comparative example 10
Growth performance comparative test
With the livestock and poultry special purpose nutrition-strengthening liquid that embodiment 4 is obtained, do Performance comparision with the water-soluble nourishing additive agent of powdery existing on market, 200 7 aa broiler chicken, are divided into two groups immediately, often organize 100, raise in cages, free choice feeding and drinking-water, routine immunization; Fortification liquid and the water-soluble nourishing additive agent of powdery be 100mL(100g respectively) be watered 100L(and make active constituent content identical), be used in conjunction 5d, use once week about, until experiment terminates; During 35 age in days, weigh on an empty stomach, statistics feed intake, calculates average daily gain, average daily ingestion amount, feedstuff-meat ratio, and carries out statistical analysis to two groups of growth performances.Duration of test respectively organizes pig growth performance basic condition in table 2, as shown in table 2, wherein in table 2, and average daily gain (g/d)=(average weight-average initial weight) (g)/28(d); Feedstuff-meat ratio=average daily ingestion amount (g/d)/average daily gain (g/d).
Table 2
| Project | Fortification liquid group | The water-soluble nourishing additive agent group of powdery | Statistical analysis (P) |
| Average initial weight (g) | 104.3 | 103.5 | 0.259 |
| Average weight (g) | 1780.4 | 1620.8 | 0.043 |
| Average daily gain (g/d) | 59.9 | 54.2 | 0.038 |
| Average daily ingestion amount (g/d) | 125.6 | 115.7 | 0.026 |
| Feedstuff-meat ratio | 2.10 | 2.14 | 0.385 |
Result shows, to throw something and feed the nano-emulsion nutrient solution of identical active ingredient kind and dosage and the water-soluble nourishing additive agent of powdery, nano-emulsion nutrient solution group shows more excellent production performance, wherein average daily ingestion amount, average daily gain, 35 age in days body weight significantly increase (P < 0.05), and feedstuff-meat ratio reduces on year-on-year basis.
The invention has the beneficial effects as follows:
1, the livestock and poultry special purpose nutrition-strengthening liquid security prepared of the present invention is good;
2, the livestock and poultry special purpose nutrition-strengthening liquid that prepared by the present invention is oil-in-water type nano-emulsion, for isotropic thermodynamic stable system, significantly improve the dissolubility of vitamin in water, dispersiveness and dialysis performance, liposoluble vitamin can be made in stomach and intestine to discharge rapidly, good contact is had with intestines and stomach upper strata mucous layer, by GI epithelium mucomembranous cell interstitial, enter blood circulation, thus change vitamin physiological and biochemical procedure in vivo, and eliminate the difference absorbed between individuality, improve bioavilability;
3, preparation method proposed by the invention is simple, do not need high-pressure homogeneous, heat, cool contour energy consumption process, production cost is low, is applicable to suitability for industrialized production.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (1)
1. a broiler chicken special purpose nutrition-strengthening liquid, is characterized in that, described broiler chicken special purpose nutrition-strengthening liquid is O/W type nanoemulsion, by weight percentage, comprises following component: Tween80 20%, PEG400 4%, Cobastab
10.3%, Cobastab
20.2%, Cobastab
60.3%, Cobastab
120.01%, vitamin C 1.5%, vitamin K
30.1%, niacinamide 1.5%, calcium pantothenate 1.2%, inositol 1.2%, biotin 0.025%, vitamin A 1.2%, vitamin D
30.3%, vitamin E 0.6%, methionine 0.15%, lysine 0.6%, threonine 0.3%, arginine 0.3%, taurine 1.7%, potassium sorbate 0.3%, all the other are purified water; Be prepared from according to following step:
1, measuring 700mL purified water is placed in container, selects emulsifying agent Tween80, PEG400, adds in container, slowly stir;
2, to vitaminize B in container
1, Cobastab
2, Cobastab
6, Cobastab
12, vitamin C, vitamin K
3, niacinamide, calcium pantothenate, inositol, biotin, methionine, lysine, threonine, arginine, taurine and potassium sorbate, slow stirring and dissolving;
3, by vitamin A, vitamin D
3, vitamin E adds container, slowly stir 10 ~ 30min, form transparent supernatant liquid;
4, add water to 1000mL, stir evenly the nano-emulsion that gets product.
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| CN201310015520.5A CN103082091B (en) | 2013-01-15 | 2013-01-15 | Special nutrition strengthening solution for livestock and poultry and preparation method thereof |
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| CN201310015520.5A CN103082091B (en) | 2013-01-15 | 2013-01-15 | Special nutrition strengthening solution for livestock and poultry and preparation method thereof |
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| CN103082091B true CN103082091B (en) | 2015-05-13 |
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| CN103392929B (en) * | 2013-07-19 | 2014-11-05 | 广州海因特生物技术有限公司 | Premixing agent for controlling water content of finished fish and shrimp pellet feed product and using method of premixing agent |
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| CN104304797B (en) * | 2014-10-30 | 2017-10-13 | 四川中农致远生物科技有限公司 | A kind of manufacturing process of livestock and poultry B B-complex nano-emulsion |
| CN105412124A (en) * | 2015-12-03 | 2016-03-23 | 重庆布尔动物药业有限公司 | Veterinary vitamin AD3E emulsion and preparation method thereof |
| CN106036034A (en) * | 2016-05-20 | 2016-10-26 | 四川爱客信生物科技有限公司 | Mixed liquid additive for feed and preparation method of mixed liquid additive |
| CN106071113A (en) * | 2016-06-17 | 2016-11-09 | 厦门惠盈动物科技有限公司 | A kind of emulsifying nanometer vitamin premix material and preparation method thereof |
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| CN111227119A (en) * | 2020-01-14 | 2020-06-05 | 开封嘉骏生物科技有限公司 | Production formula and preparation method of composite vitamin nanoemulsion for livestock and poultry |
| CN114601846A (en) * | 2020-12-03 | 2022-06-10 | 河北科星药业有限公司 | Complex vitamin amino acid injection for livestock and preparation method and application thereof |
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| CN101103776B (en) * | 2007-08-07 | 2010-06-23 | 江南大学 | Method for producing semi-moist sucking pig feed |
| CN101669578B (en) * | 2009-09-24 | 2011-09-21 | 北京福乐维生物技术有限公司 | High efficiency livestock and poultry nutrition replenisher and preparation method thereof |
| CN102048750A (en) * | 2009-11-04 | 2011-05-11 | 天津瑞普生物技术股份有限公司 | Amino acid-contained complex multivitamin microemulsion preparation and preparation method thereof |
| CN101766261B (en) * | 2010-01-11 | 2011-09-28 | 广东大华农动物保健品股份有限公司 | Nano nutrient microemulsion and preparation method thereof |
| CN102144727B (en) * | 2011-01-27 | 2013-04-03 | 保定冀中药业有限公司 | Functional liquid vitamin premix and preparation method thereof |
| CN102326676A (en) * | 2011-09-30 | 2012-01-25 | 佛山市正典生物技术有限公司 | Complex vitamin molecule microemulsion and preparation method thereof |
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Denomination of invention: A special Food fortification liquid for livestock and poultry and its preparation method Effective date of registration: 20230707 Granted publication date: 20150513 Pledgee: China Construction Bank Corporation Guangzhou Development Zone Branch Pledgor: GUANGZHOU GREAT BIOLOGICAL TECHNOLOGY CO.,LTD. Registration number: Y2023980047817 |