CN102048750A - Amino acid-contained complex multivitamin microemulsion preparation and preparation method thereof - Google Patents
Amino acid-contained complex multivitamin microemulsion preparation and preparation method thereof Download PDFInfo
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- CN102048750A CN102048750A CN2009100711337A CN200910071133A CN102048750A CN 102048750 A CN102048750 A CN 102048750A CN 2009100711337 A CN2009100711337 A CN 2009100711337A CN 200910071133 A CN200910071133 A CN 200910071133A CN 102048750 A CN102048750 A CN 102048750A
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Abstract
The invention relates to a suspension applicable to the direct water drinking of animals and a preparation method thereof, wherein the suspension comprises 2-30 parts of multivitamin composition, 1-10 parts of amino acid, 5-40 parts of emulsifier, 0.1-10 parts of auxiliary emulsifier and 0.1-2 parts of antioxidant. Under the condition of stirring, the components are mixed according to a certain sequence to prepare a nano-microemulsion and/or submicron-microemulsion complex multivitamin solution, the grain diameter of each component in the solution is smaller than 100nm, and the obtained homogeneous microemulsion preparation has stable quality.
Description
Technical field
The invention belongs to the veterinary additive technical field, be particularly related to and comprise a kind of suspensoid that is applicable to animal directly-drinking and preparation method thereof, this suspensoid comprises one or more aminoacid, one or more vitamin, and the gained suspensoid is nanometer microemulsion suspensoid and/or submicron breast suspensoid.
Background technology
Feed additive is as the scientific and technological core of feed industry, and along with the development of feed industry, the kind of additive is various day by day, is to improve one of effective measures of producing animal products quantity and quality in the modern farming.Vitamin be the most frequently used also be a most important class feed additive.The whole world is used for about 120,000 tons of the vitamin of feedstuff, lists supporting one's family of feed additive in and have more than 16 kinds, and the volume of production of domestic feeding compound vitamin has occupied the second in the world.During intensification was produced, fowl was relatively more responsive to the shortage of vitamin, in the diet based on corn and bean cake, needed to add vitamin A, vitamin D usually
3, vitamin E, vitamin K, vitamin B
2, nicotinic acid, pantothenic acid, choline chloride and vitamin B
12Aminoacid is to constitute proteinic ultimate unit.Each seed amino acid all is indispensable concerning the poultry body, especially those can not be in the poultry body by poultry histiocyte oneself synthetic or aggregate velocity can not satisfy the essential amino acids of body needs, must replenish by feedstuff.The common essential amino acids of various adult poultry has 8 kinds: isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine, but different growth stage balances of different livestock and poultry species, poultry or the amino acid requirement supplied are also inequality.
For poultry, product all is by directly adding vitamin, aminoacid in feedstuff or drinking-water, satisfying the demand of the nutrient substance of different growth stages mostly on the market.Above-mentioned addition manner has certain effect, but effect not exclusively makes culturist and nutritionist satisfaction, and main cause is as follows:
1, most of vitamin, partial amino-acid are very responsive to surrounding such as temperature, humidity, oxidation, reduction, light and pH value etc., in the feedstuff course of processing, the tiring of vitamin, amino acid whose content loss are very big, giving the clinical result who causes is vitamin, the aminoacid that has added capacity in the feedstuff on the one hand, but clinically still might show vitamin, amino acid starvation disease, and add the sensitivity of vitaminized feedstuff owing to its vitamin, the storage life of feedstuff is all very short; Be in order to remedy extraneous factor to vitamin, amino acid whose loss, in Feed Manufacturing, to double to add these nourishing additive agents, having caused the increase greatly of adding cost on the other hand.
2, the used vitamin of feedstuff industry all is micron-sized powdered granule both at home and abroad, and fatsoluble vitamin exists with emulsifying micron order oil droplet form, and the vitamin of this form and the affinity of gastro-intestinal Fluid are very low, and absorbance just reduces greatly.
3, the Aquavite that exists on the market, compound amino-acid powder are many is carrier with the glucose, and life-time service easily causes growing of antibacterial.
For homogeneity, stability, the dispersibility that improves nutritive additive content in product, improve the affinity of additive and water and intestinal juice, exploitation submicron, nanometer formulation are important research directions.Nanometer suspension preparation, submicron mixed suspension preparation have caused pharmaceutical preparation scholar's extensive attention in recent years.Nano material is familiar with by people just gradually as a kind of new state that material exists, and its scientific value and application prospect are attracting increasing people's research.The novel form of developing a kind of suitable animal use just seems more urgent, and nano suspension, submicron suspensoid are expected to solve the problem that above-mentioned nourishing additive agent exists as a kind of novel form, improve the livestock and poultry cultivation benefit greatly.
Summary of the invention
The object of the present invention is to provide a kind of suspensoid that is applicable to animal directly-drinking and preparation method thereof, solve the problem of fatsoluble vitamin, aminoacid content heterogeneity, easy degraded, bad dispersibility in product, be intended to improve the affinity of additive and water and intestinal juice, improve vitamin, amino acid whose service efficiency and value.
For achieving the above object, the inventor passes through extensively suspending agent, the stabilizing agent of screening fatsoluble vitamin, the antioxidant that vitamin and aminoacid are general, and the investigation with preparation stability is groped in the investigation repeatedly of process preparation process, pay a large amount of creative works, obtained following embodiment:
1, a kind ofly contains amino acid whose compound various vitamins microemulsion formulation, it is characterized in that containing in the described preparation: the emulsifying agent of the multivitamin compositions of 2-30 part, the aminoacid of 1-10 part, 5-40 part, 0.1-10 the co-emulsifier of part, the antioxidant of 0.1-2 part is based on the gross weight meter of said preparation.
2, above-mentioned described preparation is a suspensoid, is selected from nano suspension and/or submicron suspensoid.
3, described a kind of amino acid whose compound various vitamins microemulsion formulation that contains is characterized in that described vitamin is selected from poultry fatsoluble vitamin commonly used and in the water soluble vitamins one or more.
4, described a kind of amino acid whose compound various vitamins microemulsion formulation that contains, it is characterized in that described aminoacid be selected from poultry commonly used various amino acid whose one or two or more kinds.
5, described a kind of amino acid whose compound various vitamins microemulsion formulation that contains is characterized in that described emulsifying agent is selected from one or two or more kinds of sucrose fatty acid ester, dodecyl sodium sulfate, sorbitan mono-laurate, sorbitan monostearate, sorbitan monooleate, polyoxyethylene 20 sorbitan monooleate, soybean lecithin etc.
6, described a kind of amino acid whose compound various vitamins microemulsion formulation that contains is characterized in that described co-emulsifier is selected from one or two or more kinds in glycine, PEG400, PEG4000, ethylene glycol, propylene glycol, the glycerol etc.
7, described a kind of amino acid whose compound various vitamins microemulsion formulation that contains is characterized in that described antioxidant is selected from one or two or more kinds in sodium sulfite, hydroxyl Loprazolam sodium, cysteine, glutathion, octyl gallate, the butylated hydroxyarisol etc.
8, of the present inventionly contain amino acid whose compound various vitamins microemulsion formulation, realize by following technical step:
A. an amount of pure water is through boiling and logical nitrogen is removed dissolved oxygen in the water;
B. in above-mentioned pure water, drip emulsifying agent and co-emulsifier while stirring, be cooled to then and get A liquid below 35 ℃;
C. in A liquid, add the fatsoluble vitamin of aequum while stirring, after stirring, get B liquid;
D. with aminoacid, water soluble vitamins (vitamin B
12, except the biotin, folic acid) fully dissolve with the purified water in the A step, make solution, regulate pH value to 4-7 with 10%NaOH solution, C liquid;
E. get D liquid after fully dissolving antioxidant with the purified water in the A step;
F. C liquid is slowly added in the above-mentioned B liquid, fully behind the mix homogeneously, add D liquid, standardize solution promptly gets composite Nano multidimensional microemulsion formulation.
Animal has not only kept vitamin, the whole effective ingredient of amino acid starting material medicine with the liquid suspension of vitamin, amino acid composition, because " particle diameter " greatly dwindles, the free diffusion velocity of releasing of effective ingredient greatly improves, not only improve bioavailability, also saved the use of a large amount of nourishing additive agent raw materials.
Because animal is used the raising of compound vitamin suspensoid quality and bioavailability, adding " microgranule " strengthens the gastrointestinal adsorption, the time of staying also can increase in gastrointestinal, helps the absorption to the nutrient substance active component, will inevitably improve the nutritional supplementation effects of poultry.
Suspensoid drinking-water is convenient, and dispersion of medicine can not produce the excessive situation of local concentration, has not only improved bioavailability of medicament, and greatly facilitates raiser's administration.
The specific embodiment
Following examples are in order to embodiment preferred of the present invention to be described, ability technical staff should be appreciated that following description shall not be applied to restriction protection scope of the present invention.
Embodiment 1 nanometer suspension compound vitamin oral liquid compounding
One, get the raw materials ready:
Take by weighing each raw material for standby by the recipe quantity in the following table 1.
Every batch of prescription of table 1 nanometer suspension compound vitamin oral liquid
| Title | Consumption/40L |
| Vitamin A | 3.6 hundred million Iu |
| Vitamin D 3Oil | 4,000 ten thousand Iu |
| Vitamin B2-5-phosphate ester sodium | 140g? |
| Vitamin E oil | 800g? |
| The hydrochloric acid vitamin B 1 | 80g? |
| Vitamin B 6 | 40g? |
| Vitamin B 12 | 0.8g? |
| Vitamin C | 480g? |
| Nicotiamide | 320g? |
| Biotin | 0.8g? |
| Vitamin K 3 | 120g? |
| Polysorbate-80 | 16kg? |
| Sodium sulfite | 3.2kg? |
| Glycine | 4kg? |
| Butylated hydroxyarisol | 4g? |
| ?PEG400? | 4kg? |
| Threonine | 20g? |
| Methionine | 50g? |
| Lysine | 40g? |
| Tryptophan | 25g? |
| Phenol | 120g? |
| Purified water | Be settled to 40L |
Two, preparation
2.1 an amount of pure water through boiling and logical nitrogen is removed dissolved oxygen in the water, be cooled to below 35 ℃ A liquid;
2.2 drip polysorbate-80 while stirring in above-mentioned A liquid, the dissolving back adds glycine fully, behind the uniform mixing, adds the vitamin A vitamin D of aequum
3The vitamin E vitamin K
3, continue to stir, get C liquid;
2.3 with water-soluble (vitamin) B
1, vitamin B
2, vitamin B
6, nicotiamide, vitamin C make solution with the dissolving of A liquid, regulates pH value to 4-7 with 10%NaOH solution, D liquid;
2.4 dissolve vitamin B with small amount of ethanol
12, add then in the mixed solution of biotin, folic acid, butylated hydroxyarisol and sodium sulfite, get E liquid behind the mix homogeneously;
2.5 fully dissolve threonine, methionine, lysine, tryptophan and phenol with A liquid, make solution F;
2.6 D liquid is slowly added in the above-mentioned C liquid, fully behind the mix homogeneously, add F liquid, continue to stir, fully behind the mix homogeneously, add E liquid, be settled to 40L with the pure water after the degassing, promptly.
The investigation of embodiment 2 nanometer suspension compound various vitamins stabilities of solution
The ambient stable that gained nanometer microemulsion formulation among the embodiment 1 is carried out and the investigation of accelerated stability, the result is as shown in table 2 below.
The investigation of table 2 nanometer microemulsion compound various vitamins stability of Oral
Correlation test is the result show, the vitamin A in the nanometer microemulsion is degraded in the temperature more than 30 ℃ rapidly, and it is stable that vitamin E all keeps under different temperature; Stability test shows, V under 25 ℃ of conditions
AAnd V
EStability better.High light influence factor test shows the V in the nanometer microemulsion
AIt is destroyed to have part under high light, and the V in the nano-emulsion
EUnder high light, keep stable.Therefore the compound various vitamins oral liquid needs to store at lucifuge, shady and cool place.
The investigation of embodiment 3 nanometer suspension compound various vitamins clinical effectiveness
The nanometer suspension compound various vitamins oral liquid of preparation among the embodiment 1 and the compound various vitamins oral liquid that obtains of clinical purchase, carry out the investigation of clinical effectiveness.
Choose 5000 of the assorted chickens of a canopy meat in the process of the test, 30 ages in days, online flat supporting is divided into 2 groups at random, and test period is 15 days, and record chicken death toll takes by weighing feed consumption rate and market weight, calculates feedstuff-meat ratio.Concrete result of the test sees Table 3.More as can be seen, the resultant effect of nanometer suspension compound various vitamins is far above the common multidimensional oral liquid in market from the clinical effectiveness of different multidimensional products.
The comparison of table 3 nanometer suspension compound various vitamins oral liquid and common multidimensional oral liquid test effect
| Title | Nanometer suspension compound various vitamins oral liquid group | Common multidimensional oral liquid group |
| Adult livability | 98％ a | 92％ b |
| The chicken average daily gain | 93g/d a | 83g/d b |
| Feedstuff-meat ratio | 1∶87 a | 1∶96 b |
| The chicken group observes | The feather smoothness is good, and the later stage does not expect that excrement takes place, and does not have loose bowels | Feather is smooth, and material excrement incidence rate is low, and idol has has loose bowels |
Annotate: represent that with right upper shoulder mark a of delegation or the identical person of b difference is not remarkable, different persons represent significant difference.
Drawn by last table, nanometer suspension compound various vitamins oral liquid can effectively promote broiler to increase, and can obviously improve the broiler culture benefit.
Claims (8)
1. one kind contains amino acid whose compound various vitamins microemulsion formulation, it is characterized in that containing in the described preparation: the emulsifying agent of the multivitamin compositions of 2-30 part, the aminoacid of 1-10 part, 5-40 part, 0.1-10 the co-emulsifier of part, the antioxidant of 0.1-2 part is based on the gross weight meter of said preparation.
2. a kind of amino acid whose compound various vitamins microemulsion formulation that contains according to claim 1 is characterized in that described preparation is a suspensoid, is selected from nano suspension and/or submicron suspensoid.
3. a kind of amino acid whose compound various vitamins microemulsion formulation that contains according to claim 1 is characterized in that described vitamin is selected from poultry fatsoluble vitamin commonly used and in the water soluble vitamins one or more.
4. a kind of amino acid whose compound various vitamins microemulsion formulation that contains according to claim 1, it is characterized in that described aminoacid be selected from poultry commonly used various amino acid whose one or two or more kinds.
5. a kind of amino acid whose compound various vitamins microemulsion formulation that contains according to claim 1 is characterized in that described emulsifying agent is selected from one or two or more kinds of sucrose fatty acid ester, dodecyl sodium sulfate, sorbitan mono-laurate, sorbitan monostearate, sorbitan monooleate, polyoxyethylene 20 sorbitan monooleate, soybean lecithin etc.
6. a kind of amino acid whose compound various vitamins microemulsion formulation that contains according to claim 1 is characterized in that described co-emulsifier is selected from one or two or more kinds in glycine, PEG400, PEG4000, ethylene glycol, propylene glycol, the glycerol etc.
7. a kind of amino acid whose compound various vitamins microemulsion formulation that contains according to claim 1 is characterized in that described antioxidant is selected from one or two or more kinds in sodium sulfite, hydroxyl Loprazolam sodium, cysteine, glutathion, octyl gallate, the butylated hydroxyarisol etc.
8. prepare the described a kind of amino acid whose compound various vitamins microemulsion formulation that contains of claim 1-7, comprise following technical step:
A. an amount of pure water is through boiling and logical nitrogen is removed dissolved oxygen in the water;
B. in above-mentioned pure water, drip emulsifying agent and co-emulsifier while stirring, be cooled to then and get A liquid below 35 ℃;
C. in A liquid, add the fatsoluble vitamin of aequum while stirring, after stirring, get B liquid;
D. with aminoacid, water soluble vitamins (vitamin B
12, except the biotin, folic acid) fully dissolve with the purified water in the A step, make solution, regulate pH value to 4-7 with 10%NaOH solution, C liquid;
E. get D liquid after fully dissolving antioxidant with the purified water in the A step;
F. C liquid is slowly added in the above-mentioned B liquid, fully behind the mix homogeneously, add D liquid, standardize solution promptly gets composite Nano multidimensional microemulsion formulation.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318740A (en) * | 2011-07-15 | 2012-01-18 | 山东省农业科学院畜牧兽医研究所 | Compound amino acid multi-vitamin liquid micro-emulsion and preparation method thereof |
| CN103082091A (en) * | 2013-01-15 | 2013-05-08 | 广州格雷特生物科技有限公司 | Special nutrition strengthening solution for livestock and poultry and preparation method thereof |
| CN104304679A (en) * | 2014-10-27 | 2015-01-28 | 广东金品动物营养有限公司 | Multivitamin C, E immune polysaccharide microemulsion preparation and preparation method and application of preparation |
| CN105192307A (en) * | 2015-09-09 | 2015-12-30 | 张卢军 | Coating technology of nanoscale liquid compound vitamin amino acid for achieving stability |
| CN106721069A (en) * | 2016-11-16 | 2017-05-31 | 佛山市正典生物技术有限公司 | A kind of micro emulsion of vitamin molecules containing plants essential oil and preparation method thereof |
| CN112088990A (en) * | 2019-06-17 | 2020-12-18 | 中国农业大学 | Nano-composite vitamin oral liquid and preparation method and application thereof |
-
2009
- 2009-11-04 CN CN2009100711337A patent/CN102048750A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102318740A (en) * | 2011-07-15 | 2012-01-18 | 山东省农业科学院畜牧兽医研究所 | Compound amino acid multi-vitamin liquid micro-emulsion and preparation method thereof |
| CN103082091A (en) * | 2013-01-15 | 2013-05-08 | 广州格雷特生物科技有限公司 | Special nutrition strengthening solution for livestock and poultry and preparation method thereof |
| CN104304679A (en) * | 2014-10-27 | 2015-01-28 | 广东金品动物营养有限公司 | Multivitamin C, E immune polysaccharide microemulsion preparation and preparation method and application of preparation |
| CN105192307A (en) * | 2015-09-09 | 2015-12-30 | 张卢军 | Coating technology of nanoscale liquid compound vitamin amino acid for achieving stability |
| CN106721069A (en) * | 2016-11-16 | 2017-05-31 | 佛山市正典生物技术有限公司 | A kind of micro emulsion of vitamin molecules containing plants essential oil and preparation method thereof |
| CN112088990A (en) * | 2019-06-17 | 2020-12-18 | 中国农业大学 | Nano-composite vitamin oral liquid and preparation method and application thereof |
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Application publication date: 20110511 |