CN103081613A - Method for promoting germination of lycoris interspecific hybrid seed - Google Patents
Method for promoting germination of lycoris interspecific hybrid seed Download PDFInfo
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- CN103081613A CN103081613A CN2013100365720A CN201310036572A CN103081613A CN 103081613 A CN103081613 A CN 103081613A CN 2013100365720 A CN2013100365720 A CN 2013100365720A CN 201310036572 A CN201310036572 A CN 201310036572A CN 103081613 A CN103081613 A CN 103081613A
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Abstract
The invention discloses a method for promoting germination of a lycoris interspecific hybrid seed, which comprises the following steps of: disinfecting the lycoris interspecific hybrid seed; after the disinfection, soaking the lycoris interspecific hybrid seed in warm water at 35-40 DEG C for 5-8 hours; taking out the lycoris interspecific hybrid seed, and then immersing in 40-80mg/L gibberellin solution to accelerate germination; and after germination, taking out the lycoris interspecific hybrid seed, and sowing in a matrix for further culture, wherein the lycoris interspecific hybrid seed is the hybrid seed taking L.sprengeri as a female parent and L.longituba as a male parent, the hybrid seed taking L.albiflora as a female parent and L.sprengeri as a male parent, or the hybrid seed taking L.longituba as a female parent and L.sprengeri as a male parent. Compared with the prior art, the method greatly shortens the germination time of the lycoris interspecific hybrid seed and improves the germination rate by use of the germination accelerating means of soaking in warm water, immersing in low-concentration gibberellin and the like.
Description
Technical field
The invention belongs to field of plant variety breeding technology, relate in particular to a kind of method that promotes that Lycoris interspecific cross seed is sprouted.
Background technology
Lycoris (Lycoris) plant is perennial bulb draft, and more than 20 plant totally in the whole world, and China has 17 kinds.Lycoris plants is important flowering bulb, have concurrently medicinal and view and admire function, contain lycorine, galanthamine, ungernine etc. more than 10 in its bulb and plant alkaloid, can be used for preventing parkinsonism, treatment food poisoning, scrofula, rheumatic arthritis etc.; Its pattern is abundant, the flower type is novel, wide adaptability, strong stress resistance is loved by the people, for the cut-flower that has development potentiality, by, flower border view configuration material.
The propagation method of lycoris plants comprises: bulb separation, squama piece substrate cutting and tissue cultivation, seminal propagation etc., and wherein take the bulb separation method as main.But the reproduction rate in the mother bulb every year of growing up under natural conditions is only 1.8 times, only depends on the reproduction coefficient of nourishing and generating extremely low, and not yet forms so far large-scale production.
Lycoris plants such as short-tube lycoris (triploid), vitro of Lycoris albiflora etc. can not be normally solid, can produce viable seed and change the kinds such as brocade flower, Bulbus Lycoridis longitubae, Lycoris, and seed production are more.Seminal propagation is the important channel that ornamental plants pattern, flower type natural variation form, and under wild condition, changes the brocade flower, Bulbus Lycoridis longitubae exists and enriches the pattern variation, and this may be relevant with its natural variation of raising up seed of growing directly from seeds.
The seminal propagation method is general only for crossbreeding., should sow immediately after seed collecting without dormancy due to seed, 20 ℃ lower 15 days afterwards visible radicle expose.When sowing under natural environment, first growth cycle only has the minority seedling to extract leaf out, seedling stage portable once.
Yet, because the seed of lycoris plants belongs to Recalcitrant Seeds, very easily vitality is lost in dehydration, the germination rate that makes seed propagation also very low (Liu Zhigao. three kinds of lycoris plants Germination Characteristics researchs [J]. northern gardening .2011 (17): 90～93.).
For improving the germination rate of lycoris plants seed, researchers have launched large quantity research.By the gibberellin that utilizes higher concentration, seed is carried out pretreatment, soak 24h (Mu Hongmei as 100-1000mg/L, Xia Bing etc. the impact [J] of gibberellin on the Lycoris seed germination. northern gardening .2012. (16): 26～27.), but the Lycoris seed is more responsive to gibberellin, the gibberellin excessive concentration is also harmful to Crop development, as 50mg/L gibberellin process the sprouting that namely suppresses Bulbus Lycoridis longitubae (Liu Zhigao. three kinds of lycoris plants Germination Characteristics researchs [J]. northern gardening .2011 (17): 90～93.).
The crossbreeding work of lycoris plants starts from nineteen fifties, and there is incompatibility in distant hybridization more, and hybrid seed abortion probability is high.These characteristics are seriously restricting formation and the screening of the new ornamental types of lycoris plants.In this external distant hybridization breeding process, generally also need the distant hybridization seed is implemented embryo rescue to overcome cross-incompatibility, not only workload is large, and the embryo culture time is longer, easily pollutes in process.
Summary of the invention
The invention provides a kind of method that promotes that Lycoris interspecific cross seed is sprouted, solved the problem that in the prior art, Lycoris interspecific cross seed germinating time is long, germination rate is low.
The method that a kind of promotion Lycoris (Lycoris) interspecific cross seed is sprouted comprises:
(1) with the disinfection of Lycoris interspecific cross seed;
(2) soaked 5～8 hours in 35～40 ℃ of warm water after the sterilization;
(3) immerse again vernalization in the Gibberellins solution of 40～80mg/L after taking out;
(4) the rear taking-up of sprouting is seeded in and continues in matrix to cultivate;
Described Lycoris interspecific cross seed for change brocade flower (L.sprengeri) for maternal and Bulbus Lycoridis longitubae (L.longituba) for the hybrid seed of male parent, vitro of Lycoris albiflora (L.albiflora) for maternal with change the brocade flower for the hybrid seed of male parent or Bulbus Lycoridis longitubae for maternal with change bright and beautiful spending and be the hybrid seed of male parent.
In step (1), described method of disinfecting is:
Described Lycoris interspecific cross seed is placed in Baijunling solution soaks seed, flowing water rinses afterwards.
Because the Baijunling original liquid concentration is too large, for reducing the stimulation to Lycoris interspecific cross seed, described Baijunling solution is preferably 1000～2000 times of dilutions (if without specified otherwise, being all dilute with waters) of Baijunling stoste.Seed soaking time is preferably 1～2 hour.Adopt flowing water to rinse short-tube lycoris hybrid seed after seed soaking, the Baijunling that washes away the surface of the seed is residual; The flowing water washing time is preferably 1～3 hour.
The acquisition methods of described Lycoris interspecific cross seed is:
1) between 9～October, hybrid strain is carried out artificial pollination, gathered afterwards the hybridization fruit of mature and plump, free from insect pests in 30～50 days;
2) with clear water, the hybridization fruit that gathers is cleaned, wash away the dust of fruit surface;
3) carry out surface decontamination with tween (can select polysorbas20) or liquid detergent immersion, soak time is preferably 10～20 minutes;
4) peel off pericarp, obtain described Lycoris interspecific cross seed.
To be placed in 35～40 ℃ of warm water through the Lycoris interspecific cross seed of disinfecting and soak 5～8 hours, more preferably, soak 6 hours in 40 ℃ of warm water.The effect of emerge in worm water is softening kind of skin, to reduce the prominent resistance that breaks in the seed coat of radicle.For increasing warm water and the contact area of planting skin, during emerge in worm water, the water surface was not to have seed to be advisable.
For promoting seed germination, the present invention utilizes gibberellin to soak described Lycoris interspecific cross seed.Because the Lycoris seed is comparatively responsive to gibberellin, it is the gibberellin of 40～80mg/L that the present invention selects concentration, and for different Lycoris interspecific cross seeds, best gibberellin concentration is different.As spending as the maternal preferred gibberellin concentration of filial generation as 80mg/L to change brocade.
Sprout required air capacity for guaranteeing described Lycoris interspecific cross seed, do not affect the respiration of seed, during immersion, container is uncovered, and adds Gibberellins solution every day, seed is dipped in Gibberellins solution all the time, but the liquid level of Gibberellins solution should not have seed.
In step (3), the Lycoris interspecific cross seed that preferably will be immersed in gibberellin is placed in climatic cabinate vernalization.Germination temperature is 25～28 ℃, and germination time is 8～30 days, and ambient humidity is 70～80%, and illuminance is 30～35%, and light application time is 3～8 hours/day.More preferably, germination temperature is 25 ℃, and ambient humidity is 75%, and illuminance is 33%, and light application time is 5 hours/day.Germination time is complied with different Lycoris interspecific cross seeds and free setting.
In step (3), be 40%～60% peat in water content with the prominent Lycoris interspecific cross seed sowing of breaking in the seed coat of radicle.Peat drainage ventilation performance is good, and water retention property is strong, and the pH value of import peat is more stable, and contains the multiple nutritional components that is fit to seedling growth, and grain thickness is moderate, is conducive to the growth of nursery stock.
Test shows, method of the present invention can not only effectively promote the sprouting of Lycoris interspecific cross seed, also has facilitation to planting interior Seed germination.
Compared with prior art, beneficial effect of the present invention is:
The present invention utilizes the vernalization means such as emerge in worm water, the immersion of low concentration gibberellin, cultivation can be germinateed after 8 days to Lycoris interspecific cross seed, in 20 days, germination rate reaches 74～100%, greatly shortened the germinating time of Lycoris interspecific cross seed, improve germination rate, improved the success rate of lycoris plants interspecific cross breeding.
Description of drawings
Fig. 1 changes brocade flower and the state diagram after 1 week of Bulbus Lycoridis longitubae interspecific cross seed sprouting and 2 weeks;
Fig. 2 is vitro of Lycoris albiflora and the state diagram of changing after bright and beautiful 1 week of seeds of flowering plants intermolecular hybrid seed germination and 2 weeks;
Fig. 3 is Bulbus Lycoridis longitubae and the state diagram of changing after bright and beautiful 1 week of seeds of flowering plants intermolecular hybrid seed germination and 2 weeks;
Fig. 4 changes the state diagram of bright and beautiful flower seed sprouting after 1 day;
Fig. 5 is the state diagram of Lycoris radiata seed germination after 1 day.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
In between 9～October, hybrid strain is carried out artificial pollination, gathered afterwards the hybridization fruit of mature and plump, free from insect pests in 30～50 days.Obtain respectively to change hybridization fruit, the vitro of Lycoris albiflora (female parent) and the hybridization fruit that changes brocade flower (male parent) of brocade flower (female parent) and Bulbus Lycoridis longitubae (male parent), and Bulbus Lycoridis longitubae (female parent) and the hybridization fruit that changes brocade and spend (male parent).
Embodiment 1 changes the sprouting of brocade flower (female parent) and Bulbus Lycoridis longitubae (male parent) interspecific cross seed
(1) the brocade flower (female parent) that changes that will gather is cleaned with the rear clear water of interspecific cross fruit arrangement of Bulbus Lycoridis longitubae (male parent), soak with a little polysorbas20 or liquid detergent and carried out surface decontamination in 20 minutes, peel off pericarp, hybrid seed soaks flowing water flushing afterwards in 2 hours 3 hours with 1000 times of dilutions of Baijunling;
(2) will be placed in beaker through the hybrid seed of disinfecting, be placed in the thermostat water bath of 40 ℃, and add water in beaker and do not have seed, soak seed 8 hours;
(3) hybrid seed is taken out from beaker, dry moisture, be put in the glass culture dish that is covered with filter paper, every ware adds the Gibberellins solution of appropriate 80mg/L every day, hybrid seed is immersed in Gibberellins solution all the time, but did not have;
(4) above-mentioned culture dish is put in vernalization in 25 ℃ of temperature, humidity 75%, illuminance 33%, the light application time constant incubator of 5 hours/day, the 8th day hybrid seed begins to germinate, and in 20 days, germination rate reaches 74%;
(5) the prominent hybrid seed that breaks in the seed coat of radicle is broadcast in the dish of the cave of peat water content 50%, root growth is vigorous.As shown in Figure 1, after one week of sowing, the radicle length of hybrid seed reaches 3.5cm; After two weeks of sowing, radicle length reaches 5.5cm.
(1) vitro of Lycoris albiflora (female parent) of gathering and the interspecific cross fruit that changes brocade flower (male parent) being arranged rear clear water cleans, soak with a little polysorbas20 or liquid detergent and carried out surface decontamination in 30 minutes, peel off pericarp, seed soaks flowing water flushing afterwards in 2 hours 2 hours with 1000 times of dilutions of Baijunling;
(2) treated hybrid seed is placed in beaker, is placed in the thermostat water bath of 40 ℃, add water in beaker and do not have seed, soaked seed 6 hours;
(3) hybrid seed is taken out from beaker, dry moisture, be put in the glass culture dish that is covered with filter paper, every ware adds the Gibberellins solution of appropriate 80mg/L every day, hybrid seed is immersed in Gibberellins solution all the time, but did not have;
(4) above-mentioned culture dish is put in vernalization in 25 ℃ of temperature, humidity 75%, illuminance 33%, the light application time constant incubator of 5 hours/day, the 9th day hybrid seed begins to germinate, and in 20 days, germination rate reaches 92%;
(5) the prominent hybrid seed that breaks in the seed coat of radicle is broadcast in the dish of the cave of peat water content 50%, root growth is vigorous.As shown in Figure 2, after one week of sowing, the radicle length of hybrid seed reaches 2.2cm; After two weeks of sowing, radicle length reaches 5.0cm.
(1) Bulbus Lycoridis longitubae (female parent) of gathering and the interspecific cross fruit that changes brocade flower (male parent) being arranged rear clear water cleans, soak with a little polysorbas20 or liquid detergent and carried out surface decontamination in 15 minutes, peel off pericarp, seed soaks flowing water flushing afterwards in 1 hour 3 hours with 1000 times of dilutions of Baijunling;
(2) treated hybrid seed is placed in beaker, is placed in the thermostat water bath of 40 ℃, add water in beaker and do not have seed, soaked seed 5 hours;
(3) hybrid seed is taken out from beaker, dry moisture, be put in the glass culture dish that is covered with filter paper, every ware adds the Gibberellins solution of appropriate 60mg/L every day, hybrid seed is immersed in Gibberellins solution all the time, but did not have;
(4) above-mentioned culture dish is put in vernalization in 25 ℃ of temperature, humidity 75%, illuminance 33%, the light application time constant incubator of 5 hours/day, the 14th day hybrid seed begins to germinate, and in 20 days, germination rate reaches 100%.
(5) the prominent hybrid seed that breaks in the seed coat of radicle is broadcast in the dish of the cave of peat water content 60%, root growth is vigorous.As shown in Figure 2, after one week of sowing, the radicle length of hybrid seed reaches 4.2cm; After two weeks of sowing, radicle length reaches 6.5cm.
(1) changing of gathering cleaned with clear water after bright and beautiful flowers fruit arranges, and soaks with a little polysorbas20 or liquid detergent and carries out surface decontamination in 25 minutes, peels off pericarp, and seed soaked after 2 hours the flowing water flushing 3 hours with 1000 times of dilutions of Baijunling;
(2) change bright and beautiful flower seed and be placed in beaker treated, be placed in the thermostat water bath of 40 ℃, add water in beaker and do not have seed, soaked seed 7 hours;
The seed that (3) will change the brocade flower takes out from beaker, dries moisture, is put in the glass culture dish that is covered with filter paper, and every ware adds the Gibberellins solution of appropriate 80mg/L every day, seed is immersed in Gibberellins solution all the time, but did not have;
(4) above-mentioned culture dish is put in 25 ℃ of temperature, humidity 75%, illuminance 33%, vernalization in the constant incubator of 5 hours every days of light application time, seed began to germinate on the 6th day, and in 30 days, germination rate reaches 80%;
(5) the prominent bright and beautiful flower seed that changes that breaks in the seed coat of radicle is broadcast in the dish of the cave of peat water content 60%, root growth is vigorous, as shown in Figure 4.
(1) the Lycoris radiata fruit of gathering is arranged rear clear water clean, soak with a little polysorbas20 or liquid detergent and carried out surface decontamination in 20 minutes, peel off pericarp, seed soaks flowing water flushing afterwards in 1 hour 2 hours with Baijunling 1000 multiplying factor liquid;
(2) treated Lycoris radiata seed is placed in beaker, is placed in the thermostat water bath of 40 ℃, add water in beaker and do not have seed, soaked seed 6 hours;
(3) seed with Lycoris radiata takes out from beaker, dries moisture, is put in the glass culture dish that is covered with filter paper, and every ware adds the Gibberellins solution of appropriate 40mg/L every day, seed is immersed in Gibberellins solution all the time, but did not have;
(4) above-mentioned culture dish is put in 25 ℃ of temperature, humidity 75%, illuminance 33%, vernalization in the constant incubator of 5 hours every days of light application time, the 15th day begins to germinate, and in 35 days, germination rate reaches 85%;
(5) the prominent Lycoris radiata seed that breaks in the seed coat of radicle is broadcast in the dish of the cave of peat water content 60%, root growth is vigorous, as shown in Figure 5.
Claims (9)
1. a method that promotes that Lycoris (Lycoris) interspecific cross seed is sprouted, is characterized in that, comprising:
(1) with the disinfection of Lycoris interspecific cross seed;
(2) soaked 5～8 hours in 35～40 ℃ of warm water after the sterilization;
(3) immerse again vernalization in the Gibberellins solution of 40～80mg/L after taking out;
(4) the rear taking-up of sprouting is seeded in and continues in matrix to cultivate;
Described Lycoris interspecific cross seed for change brocade flower (L.sprengeri) for maternal and Bulbus Lycoridis longitubae (L.longituba) for the hybrid seed of male parent, vitro of Lycoris albiflora (L.albiflora) for maternal with change the brocade flower for the hybrid seed of male parent or Bulbus Lycoridis longitubae for maternal with change bright and beautiful spending and be the hybrid seed of male parent.
2. the method for claim 1, is characterized in that, in step (1), described method of disinfecting is:
Described Lycoris interspecific cross seed is placed in Baijunling solution soaks seed, flowing water rinses afterwards.
3. method as claimed in claim 2, is characterized in that, described Baijunling solution is 1000～2000 times of dilutions of Baijunling stoste.
4. method as claimed in claim 2, is characterized in that, seed soaking time is 1～2 hour.
5. the method for claim 1, is characterized in that, the flowing water washing time is 1～3 hour.
6. the method for claim 1, is characterized in that, in step (2), soaked 5～8 hours in 40 ℃ of warm water.
7. the method for claim 1, is characterized in that, in step (3), germination temperature is 25～28 ℃, and germination time is 8～30 days, and ambient humidity is 70～80%, and illuminance is 30～35%, and light application time is 3～8 hours/day.
8. method as claimed in claim 7, is characterized in that, in step (3), germination temperature is 25 ℃, and ambient humidity is 75%, and illuminance is 33%, and light application time is 5 hours/day.
9. described method as arbitrary in claim 1～8, is characterized in that, in step (4), described matrix is the peat of water content 40%～60%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106717275A (en) * | 2016-12-19 | 2017-05-31 | 庆阳敦博科技发展有限公司 | A kind of germination accelerating method of levisticum seed |
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| JP2005198607A (en) * | 2004-01-19 | 2005-07-28 | Tokyo Electric Power Co Inc:The | Method for suppressing weed |
| CN102172218A (en) * | 2011-01-26 | 2011-09-07 | 浙江大学 | Method for restoring activity of inactive lycoris seed |
| CN102696479A (en) * | 2012-04-24 | 2012-10-03 | 江苏省中国科学院植物研究所 | Method for propagating stonegarlic quickly and efficiently |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP2005198607A (en) * | 2004-01-19 | 2005-07-28 | Tokyo Electric Power Co Inc:The | Method for suppressing weed |
| CN102172218A (en) * | 2011-01-26 | 2011-09-07 | 浙江大学 | Method for restoring activity of inactive lycoris seed |
| CN102696479A (en) * | 2012-04-24 | 2012-10-03 | 江苏省中国科学院植物研究所 | Method for propagating stonegarlic quickly and efficiently |
Non-Patent Citations (1)
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| 刘志高: "三种石蒜属植物种子萌发特性研究", 《北方园艺》, no. 17, 30 September 2011 (2011-09-30), pages 90 - 93 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106717275A (en) * | 2016-12-19 | 2017-05-31 | 庆阳敦博科技发展有限公司 | A kind of germination accelerating method of levisticum seed |
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