CN103070284A - Preparation method of food rich in peanut peptide - Google Patents
Preparation method of food rich in peanut peptide Download PDFInfo
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- CN103070284A CN103070284A CN201210596311XA CN201210596311A CN103070284A CN 103070284 A CN103070284 A CN 103070284A CN 201210596311X A CN201210596311X A CN 201210596311XA CN 201210596311 A CN201210596311 A CN 201210596311A CN 103070284 A CN103070284 A CN 103070284A
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Abstract
The invention relates to a preparation method of a food rich in a peanut peptide. The method comprises the following specific steps: 1) pulping, 2) filtering and degreasing, 3) carrying out enzymolysis, 4) decoloring and deodorizing, 5) sterilizing, 6) filtering and separating, and 7) storing. The preparation method has the advantages that 1 the industralization of a peanut peptide product is implemented and products like the peanut peptide beverage as well as peanut butter and crushed peanuts containing peptide are produced initiatively; 2 the production cost is relatively low; 3 the specifity is strong and the energy consumption is low. The water saturation enzymolysis approach can be used to conduct located hydrolysis under the specific condition to produce the specific peptide, the hydrolysis progress is easy to control, high selectivity is achieved, the loss of nutrition is generally not caused, and the toxicological problem is not produced either. Furthermore, the enzyme action has specificity, can be conducted under a mild condition and is low in energy consumption.
Description
Technical field
The present invention relates to the making food field, particularly a kind of preparation method who is rich in the food of peanut peptide.
Background technology
The eighties in 20th century, in the world as the country such as Japan, Korea S, the U.S. and Europeanly all had professional polypeptide distributors to carry out industrialization to operate.Interchange and research topic relate to numerous industries that are associated with polypeptide such as biology, chemistry, pharmacy, food.In recent years, American-European and Japanese, formed widely polypeptide market.Product mainly contains two aspects, and a class is polypeptide medicine and reagent, and 100 multiple polypeptides medicines go on the market in the world, and this series products purity is very high, and price is also very expensive; Another kind ofly be the low antigen health food take active peptides as function factor and contain the polypeptide bread and cheese.
Domestic since the eighties in 20th century also Peanut Polypeptide development and application aspect research, some educational research mechanisms carry out peanut peptide research, peanut protein enzyme hydrolysis aspect is furtherd investigate, select at protease, the enzymolysis process parameter, the aspects such as hydrolyzate debitterize, desalination, separation and purification obtain certain progress.The scientific and technical personnel of report Wuhan peptide matters research institute are arranged recently under the cooperation of American scientist, utilize restriction endonuclease to modify the complex enzyme technology, peanut protein is carried out pacemaker enzyme cut processing, made Peanut Polypeptide, this can be described as the revolutionary approach that forefathers never attempted.
The production of peanut peptide mainly contains synthetic and two kinds of methods of protein extracorporeal hydrolysis.Synthetic method mainly contains chemical synthesis, synthetic, the synthetic 3 kinds of methods of enzyme process of DNA recombinant technique.Although synthetic method can be by the synthetic any active peptide of people's wish, problem is restricting its development because cost is high, the side reaction thing reaches residue more etc. at present.If recombinant DNA technology is expected to successfully, will have bright prospects.Extracorporeal hydrolysis protein is that peptide has 3 kinds of methods: acid hydrolysis, basic hydrolysis and enzymatic hydrolysis.Front two kinds of methods are simple to operation, but may have the harmful effect of nutrition and toxicity aspect, so its application has been subject to certain restriction.Protein Enzymatic Hydrolysis can position under certain conditions hydrolysis and produce specific peptide, and is easy to the controlled hydrolysis process, and has the selectivity of height, generally can not cause the loss of nutrition aspect, also can not produce the problem on the toxicity.Simultaneously enzyme effect has specificity, can carry out under temperate condition, and energy consumption is low, thereby can satisfy preferably the need of production of peptide.
The method that the water saturation enzymatic isolation method is produced the peanut peptide based food is: raw material (milky white shelled peanut) is added in proportion quantitative pure water and is polished into saturation water peanut paste solution, water saturation solution after the filtered and degreased is added specific enzyme, under 57 ℃ of constant temperatures, speed take speed as 40～45r/min stirred 30 minutes, make it fully to react enzymolysis, after taking off flavor, decolouring, degreasing, through again filtering peanut butter and the peanut beverage that obtains being rich in peptide.
Summary of the invention
For the defective that exists in the prior art, the object of the present invention is to provide a kind of method for preparing the food that is rich in peanut peptide.
For reaching above purpose, the technical scheme that the present invention takes is:
1. preparation method who is rich in the food of peanut peptide, concrete steps are as follows:
1) making beating
Blanched peanut and pure water are dropped into aseptic sander polishing pulping according to percentage by weight at 1: 3.5; (aseptic sander is the aseptic sander of 820G type of Changsha admittedly sharp pharmacy pulverizer factory)
2) filtration, degreasing
With step 1) slurries that make are delivered to filter through the pipeline of sealing, filter, degreasing;
Idiographic flow is as follows:
A, slurries filter through the 15-20 mesh sieve;
B, the slurries after will filtering are preheating to 22 ℃;
C, to add concentration according to the 0.03%-0.1% of slurries gross weight be 70% sodium phosphate trimer;
D, to add the concentration account for slurries gross weight 6-7% be 2% sodium carbonate liquor;
E, be heated to 37 ℃, reaction 2.5h;
F, under 60 ℃, carry out degreasing;
Conveyance conduit is circular all steel pipe, and the angle of inclination is 25 degree, pressure-bearing 0.25Mpa;
Filter is the 6690 type filtered and degreased machines that Hangzhou Beaune industrial filter Co., Ltd produces, Beneteau bag filter employing side is advanced side and is gone out mode, with filter liquide medium suction filter staving, enter filter from the filter bag top by ducted pressure, produce the effect of desirable Separation of Solid and Liquid.Because liquid medium is evenly distributed on the filtering surface of whole filter bag, makes the fluid distribution substantially constant in the whole aspect consistent, the negative effect of turbulent flow is little, good filtration effect.
3) enzymolysis
Will be through step 2) after slurries join the enzymolysis device, heat and keep 57 ℃ of constant temperature, add papain, concrete amount is 5500u/g, the speed take speed as 40～45r/min stirred 30 minutes, made it fully to react enzymolysis;
The enzymolysis device is that the film that Hubei Wurui Biology Engineering Co., Ltd. produces separates the Z3200 type enzymolysis device that combines with Enzymic reaction
Papain is Nanning Pang Bo bio tech ltd production, product batch number 12110206
4) decolouring, deodorization
Place decoloration device to decolour in the slurries behind the enzymolysis;
Decoloration device is the YX295-300 type decoloration device that sky, Yixing general grain and oil machinery Co., Ltd produces, in the exclusive equipment such as desolventizing device, drier, bleaching tower, deodorizing tower, crystallizing tank, heat exchanger are arranged.
The decolouring flow process is as follows:
Concrete steps are:
A, adsorption bleaching
Add respectively soft water, the atlapulgite that accounts for slurries gross weight 1.5-3.0% account for slurries gross weight 10-15% in the slurries behind enzymolysis, the concentration that accounts for slurries gross weight 0.02-0.04% is the citric acid of concentration 4.5%, it is 70-80 ℃ in temperature, pressure is 2.5-4.0kpa, decolouring 20min.
B, filtration
C, deodorization
200 ℃ of deodorization temperatures, pressure 260-650pa, the steam intake is 5-12kg/t.h, deodorization time 2-5h;
D, filtration
Under temperature is 55-60 ℃, filter, remove useless carclazyte;
5) sterilization
Boil, autoclaving
The 900/T-GX type computer full-automatic side spray HTHP conditioning-type retort (pulsed) of Zhucheng market day logical mechanical Co., Ltd production.
Parameter is as follows:
| Specification | Internal diameter (mm) | Tube length (mm) | Dischargeable capacity m 3 | Treating capacity (T) | Maximum working pressure (Mpa) | Sterilization dish number (cover) |
| 900 | 900 | 1800 | 1.3 | 0.3 | 0.35 | 2 |
6), filter, separate
Through step 5) after slurries 120-200 order all steel sieve; The slurries that obtain respectively being rich in the peanut butter of peanut peptide and be rich in peanut peptide;
7) store
Will be through step 6) peanut butter that is rich in peanut peptide that obtains and the slurries that are rich in peanut peptide are stored in the aseptic stainless cylinder of steel, storage environment be 10 ℃ ± 3 ℃ to be packed, store.
Advantage of the present invention is: 1, realized the industrialization of peanut peptide based article, taken the lead in producing peanut peptide class beverage and contain the goods such as peptide class peanut butter, peanut be broken.2, production cost is lower.3, selectivity is strong, and energy consumption is low.The water saturation enzymatic isolation method can be located under given conditions hydrolysis and be produced specific peptide, and is easy to the controlled hydrolysis process; Selectivity with height generally can not cause the loss of nutrition aspect, can not produce the problem on the toxicity yet.In addition, the enzyme effect has specificity, can carry out under temperate condition, and energy consumption is low.
The specific embodiment
Below the present invention is described in further detail.
Embodiment 1
A kind of preparation method who is rich in the food of peanut peptide, concrete steps are as follows:
1) making beating
Blanched peanut and pure water are dropped into aseptic sander polishing pulping according to percentage by weight at 1: 3.5;
2) filtration, degreasing
With step 1) slurries that make are delivered to filter through the pipeline of sealing, filter, degreasing;
Idiographic flow is as follows:
A, slurries filter through 15 mesh sieves;
B, the slurries after will filtering are preheating to 22 ℃;
C, to add concentration according to 0.03% of slurries gross weight be 70% Quadrafos;
D, adding concentration are 2%, and pH value is 11 sodium carbonate liquor;
E, be heated to 37 ℃, reaction 2.5h;
F, under 60 ℃, carry out degreasing;
3) enzymolysis
Will be through step 2) after slurries join the enzymolysis device, heat and keep 57 ℃ of constant temperature, add papain, concrete amount is 5500u/g, the speed take speed as 40r/min stirred 30 minutes, made it fully to react enzymolysis;
4) decolouring
Place decoloration device to decolour in the slurries behind the enzymolysis;
Concrete steps are:
A, adsorption bleaching
The concentration that add respectively soft water, the atlapulgite that accounts for slurries gross weight 1.5% account for slurries gross weight 10% in the slurries behind enzymolysis, accounts for slurries gross weight 0.02% is 4.5% citric acid, is 70 ℃ in temperature, and pressure is 2.5kpa, decolouring 20min.
B, filtration
C, deodorization
200 ℃ of deodorization temperatures, pressure 260pa, the steam intake is 5kg/t.h, deodorization time 2h;
D, filtration
Under temperature is 55 ℃, filter, remove useless carclazyte;
5) sterilization
Boil, autoclaving;
6), filter, separate
Through step 5) after slurries 120 order all steel sieves; The slurries that obtain respectively being rich in the peanut butter of peanut peptide and be rich in peanut peptide;
7) store
Will be through step 6) peanut butter that is rich in peanut peptide that obtains and the slurries that are rich in peanut peptide are stored in the aseptic stainless cylinder of steel, storage environment be 7 ℃ to be packed, store.
Embodiment 2
A kind of preparation method who is rich in the food of peanut peptide, concrete steps are as follows:
1) making beating
Blanched peanut and pure water are dropped into aseptic sander polishing pulping according to percentage by weight at 1: 3.5;
2) filtration, degreasing
With step 1) slurries that make are delivered to filter through the pipeline of sealing, filter, degreasing;
Idiographic flow is as follows:
A, slurries filter through 20 mesh sieves;
B, the slurries after will filtering are preheating to 22 ℃;
C, to add concentration according to 0.1% of slurries gross weight be 70% Quadrafos;
D, adding concentration are 2%, and pH value is 11 sodium carbonate liquor;
E, be heated to 37 ℃, reaction 2.5h;
F, under 60 ℃, carry out degreasing;
3) enzymolysis
Will be through step 2) after slurries join the enzymolysis device, heat and keep 57 ℃ of constant temperature, add papain, concrete amount is 5500u/g, the speed take speed as 45r/min stirred 30 minutes, made it fully to react enzymolysis;
4) decolouring
Place decoloration device to decolour in the slurries behind the enzymolysis;
Concrete steps are:
A, adsorption bleaching
The concentration that add respectively soft water, the atlapulgite that accounts for slurries gross weight 3.0% account for slurries gross weight 15% in the slurries behind enzymolysis, accounts for slurries gross weight 0.04% is 4.5% citric acid, is 80 ℃ in temperature, and pressure is 4.0kpa, decolouring 20min.
B, filtration
C, deodorization
200 ℃ of deodorization temperatures, pressure 650pa, the steam intake is 12kg/t.h, deodorization time 2-5h;
D, filtration
Under temperature is 60 ℃, filter, remove useless carclazyte;
5) sterilization
Boil, autoclaving;
6), filter, separate
Through step 5) after slurries 200 order all steel sieves; The slurries that obtain respectively being rich in the peanut butter of peanut peptide and be rich in peanut peptide;
7) store
Will be through step 6) peanut butter that is rich in peanut peptide that obtains and the slurries that are rich in peanut peptide are stored in the aseptic stainless cylinder of steel, storage environment be 13 ℃ to be packed, store.
Embodiment 3
Peptide content is measured
(1) measuring wavelength selects
Precision is measured 7% standard protein 5ml and is put in the 50mI measuring bottle, is diluted to scale with 0.9% sodium chloride solution, as stock solution.Getting 1ml puts and adds biuret reagent 4ml in the measuring tube.Get 0.9% sodium chloride solution 1ml and put and add biuret reagent 4ml in the blank tube, two manage separately behind the mixing at 30 ℃ of lower insulation 15min, place, and scan between 400～700nm with automatic ultraviolet specrophotometer, at wavelength 540nm place maximum absorption band are arranged.
(2) sample determination
Precision is measured peanut protein solution 1ml and is added biuret reagent 4ml as measuring pipe, precision is measured 7% standard protein 0.05ml and is added 0.9% sodium chloride solution 0.95ml, add the 4ml biuret reagent as standard pipe, precision is measured 0.9% sodium chloride solution 1ml and is added biuret reagent 4ml as blank tube again.Respectively manage the trap value by upper method in 540nm place mensuration, calculate content of peptides more than 0.20%.
Peanut peptide content, activity and composition:
Above best result of the test shows, Peanut Polypeptide content about 92.75%.The composition of peanut peptide is small active peptides, can be without degraded directly by intestinal absorption, can supplement the nutrients rapidly, can participate in the synthetic of human amino acid's molecule and protein directly, promote the protein active Gene Partial to play a role, strengthen immunity, can also remove interior free yl, reach anti-oxidant, prolong cell survival, reduce platelet aggregation, prevention and treatment atherosclerotic, cardiovascular and cerebrovascular disease.
Peanut peptide is of high nutritive value, and contains essential amino acid, and total nitrogen is easily absorbed by human consumption up to 14.8%; Reduce blood norcholesterol level, can effectively reduce the T-CHOL of blood plasma, the rising plasma high density lipoprotein level can reduce fat fat content; Can promote cell development to improve intelligence, the amino acid of contained more than 10 kinds of needed by human body wherein, wherein lysine can make children improve intelligence, and glutamic acid and asparatate can impel cell development and strengthen the memory capability of brain; Anti-aging, anti-early ageing, the Resveratrol content in the peanut peptide is higher than peanut protein 20%, therefore has anti-ageing, oxidation resistant function; In addition, contained catechin, lysine also has very strong anti-aging effect to human body in the peanut peptide.
Peanut peptide has following characteristics:
1, need not digest, directly absorb.Its surface has one deck to protect limb not to be subject to short enzyme, pepsin, pancreatin, amylase, digestive ferment and the acid-base material of human body, and it directly enters small intestine with complete form.Entered the human recycle system by intestinal absorption and bring into play its function.2, absorption is fast especially.Absorption enters the time of the circulatory system as the intravenous needle injection, plays a role fast.3, it has 100% characteristics that absorb, and without any refuse and excreta, can all be utilized by human body.4, active absorption forces absorption.When 5, absorbing, body energy need be do not expended, the gastrointestinal function burden can be do not increased.6, play carrier function, it can deliver to each cell of human body, tissue, official with various nutriment transportations.7, nutritious, amino acid and peptide source nutritional sufficiency contain each seed amino acid that needed by human body is wanted, and reach the recommended amounts of FAO and WHO.8, dissolubility is good, and safety is water-soluble, good stability.9, contain than beans material ANFs still less.10, features good taste, biologically active is high.11, suppress microbial growth by the adjusting to moisture.
Claims (3)
1. preparation method who is rich in the food of peanut peptide is characterized in that concrete steps are as follows:
1) making beating
Blanched peanut and pure water are dropped into aseptic sander polishing pulping according to percentage by weight at 1: 3.5;
2) filtration, degreasing
With step 1) slurries that make are delivered to filter through the pipeline of sealing, filter, degreasing;
Idiographic flow is as follows:
A, slurries filter through the 15-20 mesh sieve;
B, the slurries after will filtering are preheating to 22 ℃;
C, to add concentration according to the 0.03%-0.1% of slurries gross weight be 70% sodium phosphate trimer;
D, to add the concentration account for slurries gross weight 6-7% be 2% sodium carbonate liquor;
E, be heated to 37 ℃, reaction 2.5h;
F, under 60 ℃, carry out degreasing;
3) enzymolysis
Will be through step 2) after slurries join the enzymolysis device, heat and keep 57 ℃ of constant temperature, add papain, concrete amount is 5500u/g, the speed take speed as 40～45r/min stirred 30 minutes, made it fully to react enzymolysis;
4) decolouring
Place decoloration device to decolour in the slurries behind the enzymolysis;
Concrete steps are:
A, adsorption bleaching
The concentration that add respectively soft water, the atlapulgite that accounts for slurries gross weight 1.5-3.0% account for slurries gross weight 10-15% in the slurries behind enzymolysis, accounts for slurries gross weight 0.02-0.04% is 4.5% citric acid, it is 70-80 ℃ in temperature, pressure is 2.5-4.0kpa, decolouring 20min.
B, filtration
C, deodorization
200 ℃ of deodorization temperatures, pressure 260-650pa, the steam intake is 5-12kg/t.h, deodorization time 2-5h;
D, filtration
Under temperature is 55-60 ℃, filter, remove useless carclazyte;
5) sterilization
Boil, autoclaving;
6), filter, separate
Through step 5) after slurries 120-200 order all steel sieve; The slurries that obtain respectively being rich in the peanut butter of peanut peptide and be rich in peanut peptide;
7) store
Will be through step 6) peanut butter that is rich in peanut peptide that obtains and the slurries that are rich in peanut peptide are stored in the aseptic stainless cylinder of steel, storage environment be 10 ℃ ± 3 ℃ to be packed, store.
2. the peanut butter that is rich in peanut peptide of method according to claim 1 preparation.
3. the beverage that is rich in peanut peptide of method according to claim 1 preparation.
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| CN201210596311.XA CN103070284B (en) | 2012-12-27 | 2012-12-27 | Preparation method of food rich in peanut peptide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210596311.XA CN103070284B (en) | 2012-12-27 | 2012-12-27 | Preparation method of food rich in peanut peptide |
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| Publication Number | Publication Date |
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| CN103070284A true CN103070284A (en) | 2013-05-01 |
| CN103070284B CN103070284B (en) | 2015-05-27 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103393168A (en) * | 2013-07-23 | 2013-11-20 | 天津春发生物科技集团有限公司 | Preparation method of peanut butter |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731445A (en) * | 2010-02-06 | 2010-06-16 | 赵广彬 | Method for preparing peanut protein and peanut peptide by using low temperature peanut pulp |
| CN102150740A (en) * | 2011-04-27 | 2011-08-17 | 山东省花生研究所 | Method for preparing peanut antioxidant peptides |
| CN102379435A (en) * | 2011-09-15 | 2012-03-21 | 西南大学 | Peanut polypeptide liquid and peanut protein polypeptide beverage prepared from the peanut polypeptide liquid |
| CN102669405A (en) * | 2012-05-22 | 2012-09-19 | 新沂市嘉禾鸭业有限公司 | Preparation method of duck bone protein peptide |
-
2012
- 2012-12-27 CN CN201210596311.XA patent/CN103070284B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101731445A (en) * | 2010-02-06 | 2010-06-16 | 赵广彬 | Method for preparing peanut protein and peanut peptide by using low temperature peanut pulp |
| CN102150740A (en) * | 2011-04-27 | 2011-08-17 | 山东省花生研究所 | Method for preparing peanut antioxidant peptides |
| CN102379435A (en) * | 2011-09-15 | 2012-03-21 | 西南大学 | Peanut polypeptide liquid and peanut protein polypeptide beverage prepared from the peanut polypeptide liquid |
| CN102669405A (en) * | 2012-05-22 | 2012-09-19 | 新沂市嘉禾鸭业有限公司 | Preparation method of duck bone protein peptide |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103393168A (en) * | 2013-07-23 | 2013-11-20 | 天津春发生物科技集团有限公司 | Preparation method of peanut butter |
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