CN103060401B - Method for producing reducing sugar by hydrolyzing citric acid fermentation corn residue by using composite enzyme - Google Patents
Method for producing reducing sugar by hydrolyzing citric acid fermentation corn residue by using composite enzyme Download PDFInfo
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- CN103060401B CN103060401B CN201310003366.XA CN201310003366A CN103060401B CN 103060401 B CN103060401 B CN 103060401B CN 201310003366 A CN201310003366 A CN 201310003366A CN 103060401 B CN103060401 B CN 103060401B
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Abstract
The invention relates to a fermentation engineering raw material processing reducing sugar addition process, and specifically relates to a method for producing reducing sugar by hydrolyzing citric acid fermentation corn residue by using composite enzyme. According to the method, residual obtained after corn powder jet liquefaction and filtration is adopted as an object, and is hydrolyzed by using a composite enzyme agent, such that reducing sugar is obtained. According to the invention, citric acid fermentation core powder jet liquefaction and filtration residue is adopted as an object; and a composite enzyme preparation is prepared by using various hydrolytic enzymes, and is used for hydrolyzing residue after corn powder liquefaction, such that reducing sugar is obtained. Therefore, citric acid fermentation raw material utilization rate is improved, production grain consumption is reduced, and a purpose of production cost reduction is finally achieved.
Description
Technical field
The present invention relates to fermentation engineering Raw and process the technique that increases reducing sugar, be specifically related to Semen Maydis powder last residue after steam ejection liquefaction filters, adopt compound enzymic preparation hydrolysis to obtain the preparation method of reducing sugar.
Background technology
Citric acid (Citric acid) has another name called Citric Acid, formal name used at school 2-hydroxy propane-1,2,3 carboxylic acids.Outward appearance is white granular or white crystalline powder, and relative density is 1.6550, and odorless has pleasant strong tart flavour, and entrance is frank, tart flavour without male offspring, and safety non-toxic, is widely used as the acidic flavoring agent of food and drink; Citric acid is soluble in water, alcohol, be insoluble to the organic solvents such as ether, ester, chloroform.Commodity citric acid is mainly Citric Acid, usp, Anhydrous Powder and Citric acid monohydrate Food grade, and the former is crystallization in the aqueous solution higher than 36.6 ℃, and the latter is crystallization in lower than 36.6 ℃ of aqueous solution.
The intensive degree of world's citric acid industry is very high, external in removing, and external main citric acid manufacturers only has 5～6 (U.S., Austria, Canada, Brazil etc.), and the output of China is maximum, accounts for world wide production 70% left and right.Within 2011, annual China citric acid ultimate production reaches 940,000 tons, and what account for Gross World Product is genuine big producing country from output.The intensive world lead level that reached of China's citric acid industry.Due to the raising of production-scale expansion and automatic and mechanical degree, a current line direct labor's labour productivity has reached 150 tons/people/year, before 10 years, improves approximately 10 times, has reached the world and has first intake.The raw material of China's citric acid fermentation is corn, average 1.7-1.8 ton corn can be produced 1 ton of citric acid, produce the Semen Maydis grit of 300-350 kilogram simultaneously, and Semen Maydis grit can only be processed as cheap feedstuff raw material after drying and crushing processing treatment, the reducing sugar in Semen Maydis grit is not fully used like this.With production level in 2011, calculate, the annual corn for citric acid production reaches ten thousand tons of 170-180, and forms the corn waste residue of 510,000 tons of left and right, and in waste residue, contains total reducing sugar 36.3%, robust fibre 13%, crude protein 30%, crude fat 15-16%.Carbohydrate in maize raw material being all transformed into fermentable glucide is as can be seen here the key reducing production costs.
Summary of the invention
It is object that Semen Maydis powder last residue after steam ejection liquefaction filters in citric acid fermentation is take in the present invention, apply multiple lytic enzyme and be mixed with compound enzymic preparation, residue after Semen Maydis powder liquefaction is hydrolyzed, thereby acquisition reducing sugar, improve the raw material availability of citric acid fermentation, reduce and produce grain consumption, the final object reducing production costs that arrives.
Utilize combinative enzyme hydrolysis citric acid fermentation Semen Maydis grit to obtain a method for reducing sugar, concrete steps comprise:
(1) Semen Maydis powder in citric acid fermentation is carried out to steam ejection liquefaction, after press filtration, obtain Semen Maydis grit, add water and obtain slurries, add solid NaOH to regulate the pH value of slurries, reheat to 50 ℃-60 ℃;
(2) compound enzymic preparation is put in the slurries of heating, kept temperature to stir hydrolysis;
(3) slurries after hydrolysis are filtered, obtain the filtrate of containing reducing sugar;
The weight fraction that the middle compound enzymic preparation of described step (2) accounts for Semen Maydis grit is 0.05%-0.15%(W/W);
In described step (2), the feed composition of compound enzymic preparation by weight: cellulase 60-70 part, zytase 5-15 part, middle temperature amylase 10-30 part, saccharifying enzyme 5-15 part.
Wherein, the moisture content of the Semen Maydis grit obtaining after press filtration in described step (1) is 45wt%-55wt%, is conducive to like this enzyme digestion reaction.
In described step (1), the ratio of Semen Maydis grit and water is 1:3-5(W/W), thereby carry out Semen Maydis grit, size mixing, if affect the stripping of reducing sugar higher than 1:3, if cause the increase of water consumption lower than 1:5, subsequent technique cannot be used and cause certain waste.
In described step (1), slurries add solid NaOH to regulate pH value for 5.0-6.0, and this pH value scope is the optimum enzymatic hydrolysis condition of prozyme.
In described step (1), slurries are heated to 50 ℃-60 ℃, this temperature range is optimum hydrolysis temperature, if temperature can make enzyme lose hydrolytic activity higher than 60 ℃, and can extending enzyme hydrolysis time lower than 50 ℃.
Hydrolysis time is 3-5 hour in described step (2), and the slurries after hydrolysis filter, and obtains the filtrate of containing reducing sugar, and reducing sugar quality percentage composition reaches 2.5%-4.0%.
Each enzyme in described prozyme is suitability for industrialized production all, can buy respectively each enzyme and then be mixed in proportion use.
These four kinds of enzymes in described prozyme have synergy, make in Semen Maydis grit with starch release in starch granules that Mierocrystalline cellulose and hemicellulose are combined out, and then become reducing sugar by amylase with Glucoamylase hydrolysis.
In described compound enzymic preparation, the vigor of each single enzyme raw material used is: in cellulase, carboxymethylcelluloenzyme enzyme activity is 10-15 ten thousand IU/ gram, in zytase, hemicellulose enzyme activity is 3-4 ten thousand IU/ gram, in middle temperature amylase, alpha-amylase activity is 6-8 ten thousand IU/ gram, and in saccharifying enzyme, enzyme activity is 80-100 ten thousand IU/ gram.Only have the energy value of these four kinds of dhdps enzymes to be controlled within the scope of this, and press the feed composition proportioning mixing of compound enzymic preparation, just can make compound enzymic preparation perform to best effect.
The present invention with Semen Maydis powder in citric acid fermentation through steam ejection liquefaction, after filtering, last residue is object, apply multiple lytic enzyme and be mixed with compound enzymic preparation, residue after Semen Maydis powder liquefaction is hydrolyzed, thereby obtains reducing sugar, reached like this comprehensive utilization of maize raw material, increasable economic benefit is obvious, improve the utilization ratio of citric acid fermentation raw material simultaneously, reduced production grain consumption, finally reached the object reducing production costs.
Embodiment
Embodiment 1
Utilize combinative enzyme hydrolysis citric acid fermentation Semen Maydis grit to obtain a method for reducing sugar, concrete steps comprise:
(1) Semen Maydis powder in citric acid fermentation is carried out to steam ejection liquefaction, after press filtration, obtain the Semen Maydis grit that 1 ton of moisture content is 45%, add 3 tons of water to obtain slurries, add solid NaOH to regulate the pH value to 5.0 of slurries, reheat 50 ℃;
(2) compound enzymic preparation 0.5 Kg is put in the slurries of heating, keep temperature 50 C to stir hydrolysis 3 hours;
(3) slurries after hydrolysis are filtered, obtain the filtrate of containing reducing sugar, wherein the quality percentage composition of reducing sugar is 2.5%.
The feed composition of described compound enzymic preparation is by weight: after cellulase 0.35Kg, zytase 0.025 Kg, middle temperature amylase 0.1 Kg and saccharifying enzyme 0.025 Kg mix, use.
In described compound enzymic preparation, the vigor of each single enzyme raw material used is: in cellulase, carboxymethylcelluloenzyme enzyme activity is 100,000 IU, in zytase, hemicellulose enzyme activity is 40,000 IU, in middle temperature amylase, alpha-amylase activity is 80,000 IU, and in saccharifying enzyme, enzyme activity is 800,000 IU.
Embodiment 2
Utilize combinative enzyme hydrolysis citric acid fermentation Semen Maydis grit to obtain a method for reducing sugar, concrete steps comprise:
(1) Semen Maydis powder in citric acid fermentation is carried out to steam ejection liquefaction, after press filtration, obtain the Semen Maydis grit that 1 ton of moisture content is 50%, add 5 tons of water to obtain slurries, add solid NaOH to regulate the pH value to 5.5 of slurries, reheat 60 ℃;
(2) compound enzymic preparation 1Kg is put in the slurries of heating, keep temperature 60 C to stir hydrolysis 4 hours;
(3) slurries after hydrolysis are filtered, obtain the filtrate of containing reducing sugar, wherein the quality percentage composition of reducing sugar is 4.0%.
The feed composition of described compound enzymic preparation is by weight: after cellulase 0.6Kg, zytase 0.15 Kg, middle temperature amylase 0.1 Kg and saccharifying enzyme 0.15 Kg mix, use.
In described compound enzymic preparation, the vigor of each single enzyme raw material used is: in cellulase, carboxymethylcelluloenzyme enzyme activity is 120,000 IU, in zytase, hemicellulose enzyme activity is 30,000 IU, in middle temperature amylase, alpha-amylase activity is 70,000 IU, and in saccharifying enzyme, enzyme activity is 1,000,000 IU.
Embodiment 3
Utilize combinative enzyme hydrolysis citric acid fermentation Semen Maydis grit to obtain a method for reducing sugar, concrete steps comprise:
(1) Semen Maydis powder in citric acid fermentation is carried out to steam ejection liquefaction, after press filtration, obtain the Semen Maydis grit that 1 ton of moisture content is 55%, add 4 tons of water to obtain slurries, add solid NaOH to regulate the pH value to 6.0 of slurries, reheat 55 ℃;
(2) compound enzymic preparation 1.5Kg is put in the slurries of heating, keep 55 ℃ of temperature to stir hydrolysis 5 hours;
(3) slurries after hydrolysis are filtered, obtain the filtrate of containing reducing sugar, wherein the quality percentage composition of reducing sugar is 3.0%.
The feed composition of described compound enzymic preparation is by weight: after cellulase 1Kg, zytase 0.1 Kg, middle temperature amylase 0.25 Kg and saccharifying enzyme 0.15 Kg mix, use.
In described compound enzymic preparation, the vigor of each single enzyme raw material used is: in cellulase, carboxymethylcelluloenzyme enzyme activity is 150,000 IU, in zytase, hemicellulose enzyme activity is 3.5 ten thousand IU, in middle temperature amylase, alpha-amylase activity is 60,000 IU, and in saccharifying enzyme, enzyme activity is 900,000 IU.
Embodiment 4
Utilize combinative enzyme hydrolysis citric acid fermentation Semen Maydis grit to obtain a method for reducing sugar, concrete steps comprise:
(1) Semen Maydis powder in citric acid fermentation is carried out to steam ejection liquefaction, after press filtration, obtain the Semen Maydis grit that 1 ton of moisture content is 52%, add 4 tons of water to obtain slurries, add solid NaOH to regulate the pH value to 5.3 of slurries, reheat 57 ℃;
(2) compound enzymic preparation 1.2Kg is put in the slurries of heating, keep 57 ℃ of temperature to stir hydrolysis 3.5 hours;
(3) slurries after hydrolysis are filtered, obtain the filtrate of containing reducing sugar, wherein the quality percentage composition of reducing sugar is 3.5%.
The feed composition of described compound enzymic preparation is by weight: after cellulase 0.65Kg, zytase 0.15Kg, middle temperature amylase 0.25 Kg and saccharifying enzyme 0.15 Kg mix, use.
In described compound enzymic preparation, the vigor of each single enzyme raw material used is: in cellulase, carboxymethylcelluloenzyme enzyme activity is 130,000 IU, in zytase, hemicellulose enzyme activity is 40,000 IU, in middle temperature amylase, alpha-amylase activity is 6.5 ten thousand IU, and in saccharifying enzyme, enzyme activity is 850,000 IU.
From embodiment 1-4, can find out through hydrolysis rear slurry and filter, obtain the filtrate of containing reducing sugar, wherein the quality percentage composition of reducing sugar is up to 4.0%, Semen Maydis grit per ton like this produces reducing sugar and reaches 49.8 kilograms, if calculate with glucose acid invert ratio 95%, corn per ton can be given birth to 47.39 kilograms of citric acids by fecund, with 5000 yuan/ton of calculating of citric acid price, corn per ton increases by 236.95 yuan of output values, the amount that current China only consumes corn citric acid production year reaches ten thousand tons of 170-180, and year is newly-increased up to ten thousand yuan of 40281.5-42651.Improve like this raw material availability of citric acid fermentation, reduced production grain consumption, finally reached the object reducing production costs.
Claims (2)
1. utilize combinative enzyme hydrolysis citric acid fermentation Semen Maydis grit to obtain a method for reducing sugar, it is characterized in that: concrete steps comprise:
(1) Semen Maydis powder in citric acid fermentation is carried out to steam ejection liquefaction, after press filtration, obtain Semen Maydis grit, add water and obtain slurries, add solid NaOH to regulate the pH value of slurries, reheat to 50 ℃-60 ℃;
(2) compound enzymic preparation is put in the slurries of heating, kept temperature to stir hydrolysis;
(3) slurries after hydrolysis are filtered, obtain the filtrate of containing reducing sugar;
The moisture content of the Semen Maydis grit obtaining after press filtration in described step (1) is 45wt%-55wt%;
In described step (1), the pH value of slurries is 5.0-6.0;
The weight fraction that the middle compound enzymic preparation of described step (2) accounts for Semen Maydis grit is 0.05%-0.15%;
In described step (2), the feed composition of compound enzymic preparation by weight: cellulase 60-70 part, zytase 5-15 part, middle temperature amylase 10-30 part, saccharifying enzyme 5-15 part;
In described compound enzymic preparation, the vigor of each single enzyme raw material used is: in cellulase, carboxymethylcelluloenzyme enzyme activity is 10-15 ten thousand IU, in zytase, hemicellulose enzyme activity is 3-4 ten thousand IU, in middle temperature amylase, alpha-amylase activity is 6-8 ten thousand IU, and in saccharifying enzyme, enzyme activity is 80-100 ten thousand IU;
In described step (2), hydrolysis time is 3-5 hour.
2. the method for utilizing combinative enzyme hydrolysis citric acid fermentation Semen Maydis grit to obtain reducing sugar according to claim 1, is characterized in that: in described step (1), the W/W ratio of Semen Maydis grit and water is 1:3-5.
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| CN105603024B (en) * | 2016-03-08 | 2019-12-06 | 扬州日兴生物科技股份有限公司 | Method for extracting glucosamine from corn grit by biotransformation |
| CN109628327B (en) * | 2019-01-02 | 2022-04-01 | 河南科技学院 | Method for preparing high-activity citric acid aspergillus niger seeds by fully utilizing corn liquefaction residues |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101532004A (en) * | 2009-04-20 | 2009-09-16 | 陕西省科学院酶工程研究所 | Compound enzyme for producing reducing sugar by degrading maize straws |
| CN101597628A (en) * | 2009-07-03 | 2009-12-09 | 天津科技大学 | The method of preparing xylo-oligosaccharide by corn husk enzymatic hydrolysis |
| CN102071223A (en) * | 2009-11-25 | 2011-05-25 | 河南农业大学 | Compound enzymatic hydrolysis method for straw raw materials |
| CN102618602A (en) * | 2012-03-30 | 2012-08-01 | 吴允山 | Process for preparing sugar by performing enzymatic hydrolysis on sweet potato residues |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101532004A (en) * | 2009-04-20 | 2009-09-16 | 陕西省科学院酶工程研究所 | Compound enzyme for producing reducing sugar by degrading maize straws |
| CN101597628A (en) * | 2009-07-03 | 2009-12-09 | 天津科技大学 | The method of preparing xylo-oligosaccharide by corn husk enzymatic hydrolysis |
| CN102071223A (en) * | 2009-11-25 | 2011-05-25 | 河南农业大学 | Compound enzymatic hydrolysis method for straw raw materials |
| CN102618602A (en) * | 2012-03-30 | 2012-08-01 | 吴允山 | Process for preparing sugar by performing enzymatic hydrolysis on sweet potato residues |
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Effective date of registration: 20161031 Address after: 276500 Laiyang Road, Juxian Economic Development Zone, Shandong, No. 209, No. Patentee after: Rizhao Rijin Boyuan Biochemistry Co., Ltd. Patentee after: Tianjin University of Science & Technology Address before: 276800 west section of Xing Hai Road, Shandong, Rizhao City Patentee before: Rizhao Luxin RZBC Co., Ltd. Patentee before: Tianjin University of Science & Technology |