CN100998367A - Method for extracting rice protein and joint production of alcohol - Google Patents
Method for extracting rice protein and joint production of alcohol Download PDFInfo
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- CN100998367A CN100998367A CN 200610147768 CN200610147768A CN100998367A CN 100998367 A CN100998367 A CN 100998367A CN 200610147768 CN200610147768 CN 200610147768 CN 200610147768 A CN200610147768 A CN 200610147768A CN 100998367 A CN100998367 A CN 100998367A
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Abstract
A process for extracting protein from rice while preparing alcohol includes such steps as proportionally immersing rice in alkali solution, grinding, alkali extracting, centrifugal separation of supernatant A and deposit A, regulating the pH value of said supernatant A to 5-6, isoelectric depositing, centrifugal separation of supernatant B from deposit B, airflow drying of deposit B, pulverizing to obtain rice protein, proportionally mixing deposit A, supernatant B and nutrients, adding alpha-amylase, beta-amylase and yeast, and fermenting to prepare alcohol.
Description
Technical field
The present invention relates to a kind of is the method that raw material is produced rice protein and alcohol with the rice.
Background technology
Nearly 200,000,000,000 kilograms of China's paddy annual production accounts for about 35% of Gross World Product, occupies first place in the world.Wherein the output of early rice accounts for about 1/4 of paddy total output.Current, national grain supply and demand is in a basic balance, and the rice total amount is abundant.Poor rice morning, the old rice of quality is cold-shouldered, and the stock is full, can not sell, and causes the serious waste and the enormous economic loss of grain resource.These edible qualities not high morning amylose content height in the rice, so be fit to very much the fermenting and producing fuel alcohol.Fuel alcohol adds in the gasoline by proper ratio as a kind of regenerative resource, can improve the octane number of gasoline, thereby reduces the discharging of carbon monoxide and hydrocarbon in the vehicle exhaust, reduces environmental pollution.Present stage, the key factor of restriction Production of Fuel Ethanol is the cost problem.The byproduct of exploitation high added value is strengthened raw material is fully utilized, and is the effective way that reduces its cost.
Rice protein accounts for about 8% of rice gross weight, has high nutrition, hypoallergenic, local flavor gentleness, can not cause peculiar properties such as ventosity.It is used more and more widely, produces rice protein powder, hydrolysed rice albumen, rice modified protein, high added value peptide, biologically active peptide, resistance protein as being used as; As the additive of numerous food, as the additive of bland, pudding, ice cream, baby food etc.
From rice, extract rice protein, the general alkali extraction and acid precipitation that adopts, as publication number is that the Chinese patent application of CN1528786A discloses the method that a kind of alkaline process extracts rice protein, and it comprises the processing step of following order: the cleaning of rice, dipping by lye, defibrination, stirring are extracted, are separated, acid is heavy, separation, drying; The concentration of the NaOH solution of described dipping by lye is 0.05-0.15mol/L, and solid-to-liquid ratio is 1: 6-8, soak time 2-3 hour; It is 11 stirrings that described stirring extraction refers to keep the pH of mixed value; Described acid is heavy to be isoelectric precipitation, promptly transfers pH to 5.5 with HCl, makes protein precipitation; Described baking temperature is limited in below 50 ℃.Use this method optimum to be, protein extracting ratio is 93.6%, and albumen (powder) purity is 87.3%, fat content 1.42%, content of ashes 1.20%, moisture 5%.Purity of protein still can not satisfy people's requirement, and the alkali lye amount of use is many, wastewater treatment difficulty, cost height.
And for example publication number be the Chinese patent application of CN1557178A disclosed a kind of be that raw material extracts rice protein and makes the process of rice starch with the rice, it is characterized in that its step is as follows:
A, immersion: rice was soaked 4～40 hours with basic ion type leaching liquor;
B, defibrination, lixiviate: soaked rice is levigate, continue lixiviate 2～20 hours in 20～90 ℃;
C, separation: the big Rice ﹠ peanut milk that lixiviate is finished separates by centrifugal separator, and starch changes subsequent processing and produces rice starch;
D, acid are sunk: the solution acid ion type coagulation liquid adjust pH with centrifugation goes out makes protein settling;
E, washing: with the heavy protein of clear water washing acid;
F, homogeneous: the protein that utilizes the high pressure homogenizer homogeneous to wash;
C, drying: the protein spraying drying powder-forming that homogeneous is good makes rice protein powder; Protein content 〉=70% wherein, moisture content 5～15%.
Use the Canon of this method to obtain the rice protein powder of protein content＞90%, the rice starch of protein content＜1.0%, but the time that consumes is long, step is various.
Rice coproduction alcohol and rice protein extract rice protein with rice and in other words with the technology of the material production alcohol behind the extraction rice protein, and be rarely seen in generation.
Summary of the invention
Technical problem to be solved by this invention provides the technology of the material fermenting and producing alcohol after a kind of alkaline process extracts rice protein and extracts albumen.
Technical problem to be solved by this invention can be achieved through the following technical solutions:
A kind of method of extracting rice protein and joint production of alcohol, it comprises the steps:
1, soak: rice concentration is 0.03-0.09mol/L aqueous slkali soaking 0.2-10h, and the solid-liquid ratio of rice and aqueous slkali is 1: 2-6;
2, alkali is carried: soaked rice defibrination, alkali are carried; 20-45 ℃ of alkali temperature raising degree, alkali is carried time 1-5h; Alkali is carried the big Rice ﹠ peanut milk of finishing separate separation of supernatant and sediment by centrifugal separator;
3, albumen powder preparation: with the 2nd step supernatant with sour adjust pH to the 5.0-6.0 isoelectric precipitation, centrifugation obtains supernatant and sediment, the sediment pneumatic conveying drying, is ground into powder and makes rice protein powder;
4, alcohol preparation: the 3rd step gained supernatant joins in the sediment that the 2nd step obtained and adds the nutrients of rice part by weight 5-30wt%, and adds Ye Huamei, carbohydrase and yeast according to existing technology fermenting and producing alcohol.
Described nutrients is a kind of in ammonium sulfate, rice meal or the corn flour.
The present invention adopts aqueous slkali to be preferably a kind of in NaOH, potassium hydroxide or the sodium carbonate.
Also can before the alkali lye lixiviate, rice be pulverized, cross the 20-80 mesh sieve.
Method of the present invention is an industrialized preparing process, can be continuously, extract rice protein simultaneously and produce alcohol from rice on a large scale.
Beneficial effect of the present invention is: the one, and coproduction alcohol and rice protein, the comprehensive utilization that has improved rice has reduced production cost; The 2nd, technology is simpler, and non-wastewater discharge in the process of extraction rice protein can not cause environmental pollution; The 3rd, the rice protein purity (butt) that extracts is more than 90%, and used alkali extract concentration is low, and albumen destroys little, and it is edible to be fit to human body.
Description of drawings
Further specify the present invention below in conjunction with the drawings and specific embodiments.
Fig. 1 is a process chart of the present invention.
The specific embodiment
For technological means, creation characteristic that the present invention is realized, reach purpose and effect is easy to understand, below in conjunction with the specific embodiment, further set forth the present invention.
A kind of method of extracting rice protein and joint production of alcohol of the present invention, in immersion process, the concentration of aqueous slkali is preferably 0.04-0.07mol/L, and soak time is preferably 0.5-5h, and solid-liquid ratio is preferably 1: 3-5.
Alkali temperature raising degree is preferably 20-40 ℃, and alkali is carried the time and is preferably 1-3h.
Nutrients adds the rice part by weight and is preferably 10-30wt%.
Embodiment 1:
The 70g rice is soaked in 10h in 140ml, the 0.05mol/L NaOH; Defibrination continues lixiviate, 20 ℃ of extraction temperatures, extraction time 5h; Rice ﹠ peanut milk is at the centrifugal 10min of 3000rpm, supernatant 1 adjust pH to 5.0, the centrifugal 10min of 3000rpm, gained sediment 2 pneumatic conveying dryings, pulverizing obtain rice protein, and the ammonium sulfate that the centrifugal sediment that obtains 1 of supernatant 2 and Rice ﹠ peanut milk merges back adding 5% carries out and adds Ye Huamei, carbohydrase and yeast according to existing technology fermenting and producing alcohol.Resulting rice protein: albumen (butt) purity 91.0%, recovery rate 29.3%; Alcohol: alcoholic strength 12.64%, remaining reduced sugar 0.37%.
Embodiment 2:
The 70g rice is soaked in 0.2h in 280ml, the 0.09mol/L sodium carbonate; Defibrination continues lixiviate, 35 ℃ of extraction temperatures, extraction time 3h; Rice ﹠ peanut milk is at the centrifugal 10min of 3000rpm, supernatant 1 adjust pH to 5.5, the centrifugal 10min of 3000rpm, gained sediment 2 pneumatic conveying dryings, pulverizing obtain rice protein, add 30% rice meal and add Ye Huamei, carbohydrase and yeast after the centrifugal sediment that obtains 1 of supernatant 2 and Rice ﹠ peanut milk merges and carries out fermenting and producing alcohol according to existing technology.Resulting rice protein: albumen (butt) purity 90.1%, recovery rate 49.3%; Alcohol: alcoholic strength 12.78%, remaining reduced sugar 0.13%.
Embodiment 3:
The 70g rice is soaked in 10h in 420ml, the 0.03mol/L potassium hydroxide; Defibrination continues lixiviate, 45 ℃ of extraction temperatures, extraction time 1h; Rice ﹠ peanut milk is at the centrifugal 10min of 3000rpm, supernatant 1 adjust pH to 6.0, the centrifugal 10min of 3000rpm, gained sediment 2 pneumatic conveying dryings, pulverize and to obtain rice protein, the centrifugal sediment that obtains 1 of supernatant 2 and Rice ﹠ peanut milk merges the back and adds 18% corn flour and add Ye Huamei, carbohydrase and yeast according to the existing technology preparation alcohol that ferments.Resulting rice protein: albumen (butt) purity 94.0%, recovery rate 34.3%; Alcohol: alcoholic strength 12.30%, remaining reduced sugar 0.17%.
Embodiment 4:
The 70g rice is soaked in 5h in 140ml, the 0.05mol/L potassium hydroxide; Defibrination continues lixiviate, 20 ℃ of extraction temperatures, extraction time 5h; Rice ﹠ peanut milk is at the centrifugal 10min of 3000rpm, supernatant 1 adjust pH to 5.0, the centrifugal 10min of 3000rpm, gained sediment 2 pneumatic conveying dryings, pulverizing obtain rice protein, and the ammonium sulfate that the centrifugal sediment that obtains 1 of supernatant 2 and Rice ﹠ peanut milk merges back adding 5% carries out and adds Ye Huamei, carbohydrase and yeast according to existing technology fermenting and producing alcohol.Resulting rice protein: albumen (butt) purity 90.3%, recovery rate 27.3%; Alcohol: alcoholic strength 12.84%, remaining reduced sugar 0.47%.
Embodiment 5:
The 70g rice is soaked in 10h in 280ml, the 0.09mol/L NaOH; Defibrination continues lixiviate, 35 ℃ of extraction temperatures, extraction time 3h; Rice ﹠ peanut milk is at the centrifugal 10min of 3000rpm, supernatant 1 adjust pH to 5.5, the centrifugal 10min of 3000rpm, gained sediment 2 pneumatic conveying dryings, pulverizing obtain rice protein, add 30% corn flour and add Ye Huamei, carbohydrase and yeast after the centrifugal sediment that obtains 1 of supernatant 2 and Rice ﹠ peanut milk merges and carries out fermenting and producing alcohol according to existing technology.Resulting rice protein: albumen (butt) purity 94.1%, recovery rate 51.3%; Alcohol: alcoholic strength 12.98%, remaining reduced sugar 0.12%.
Embodiment 6:
The 70g rice is soaked in 0.2h in 420ml, the 0.03mol/L sodium carbonate; Defibrination continues lixiviate, 45 ℃ of extraction temperatures, extraction time 1h; Rice ﹠ peanut milk is at the centrifugal 10min of 3000rpm, supernatant 1 adjust pH to 6.0, the centrifugal 10min of 3000rpm, gained sediment 2 pneumatic conveying dryings, pulverize and to obtain rice protein, the centrifugal sediment that obtains 1 of supernatant 2 and Rice ﹠ peanut milk merges the back and adds 18% rice meal and add Ye Huamei, carbohydrase and yeast according to the existing technology preparation alcohol that ferments.Resulting rice protein: albumen (butt) purity 93.0%, recovery rate 34.3%; Alcohol: alcoholic strength 12.30%, remaining reduced sugar 0.15%.
Embodiment 7
The 70g rice is pulverized, crossed 40 mesh sieves, stir to make it to be scattered in 280ml, the 0.05mol/L sodium hydroxide solution then and carry out lixiviate, 30 ℃ of extraction temperatures, extraction time 2h; Rice ﹠ peanut milk is at the centrifugal 10min of 3000rpm, supernatant 1 adjust pH to 5.6, the centrifugal 10min of 3000rpm, gained sediment 2 pneumatic conveying dryings, pulverize and to obtain rice protein, the centrifugal sediment that obtains 1 of supernatant 2 and Rice ﹠ peanut milk merges the back and adds 18% corn flour and add Ye Huamei, carbohydrase and yeast according to the existing technology preparation alcohol that ferments.Resulting rice protein: albumen (butt) purity 92.3%, recovery rate 44.3%; Alcohol: alcoholic strength 12.1 0%, remaining reduced sugar 0.17%.
More than show and described basic principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in the foregoing description and the specification just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (10)
1, a kind of method of extracting rice protein and joint production of alcohol is characterized in that it comprises the steps:
1), soaks: the rice aqueous slkali soaking;
2), alkali is carried: soaked rice defibrination, alkali are carried; 20-45 ℃ of alkali temperature raising degree, alkali is carried time 1-5h; Alkali is carried the big Rice ﹠ peanut milk of finishing separate separation of supernatant and sediment by centrifugal separator;
3), albumen powder preparation: with the 2nd) to the 5.0-6.0 isoelectric precipitation, centrifugal supernatant and the sediment of obtaining, is ground into powder and makes rice protein powder the sediment pneumatic conveying drying step supernatant with sour adjust pH;
4), alcohol preparation: the 3rd) the gained supernatant joins the 2nd the step) in the sediment that obtains of step and add the nutrients of rice part by weight 5-30wt%, and add Ye Huamei, carbohydrase and yeast according to existing technology fermenting and producing alcohol.
2, a kind of method of extracting rice protein and joint production of alcohol according to claim 1 is characterized in that: described nutrients is a kind of in ammonium sulfate, rice meal or the corn flour.
3, a kind of method of extracting rice protein and joint production of alcohol according to claim 1 is characterized in that: described aqueous slkali is a kind of in NaOH, potassium hydroxide or the sodium carbonate.
4, a kind of method of extracting rice protein and joint production of alcohol according to claim 1, it is characterized in that: the concentration of described aqueous slkali is 0.03-0.09mol/L.
5, a kind of method of extracting rice protein and joint production of alcohol according to claim 1, it is characterized in that: described soak time is 0.2-10h.
6, a kind of method of extracting rice protein and joint production of alcohol according to claim 1, it is characterized in that: solid-liquid ratio is 1: 2-6.
7, a kind of method of extracting rice protein and joint production of alcohol according to claim 1 is characterized in that: described alkali temperature raising degree is 20-45 ℃.
8, a kind of method of extracting rice protein and joint production of alcohol according to claim 1 is characterized in that: it is 1-5h that described alkali is carried the time.
9, a kind of method of extracting rice protein and joint production of alcohol according to claim 1, it is characterized in that: it is 5-30wt% that the nutrients of interpolation accounts for the rice part by weight.
10, a kind of method of extracting rice protein and joint production of alcohol according to claim 1 is characterized in that: before lixiviate rice is pulverized, crossed the 20-80 mesh sieve.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006101477687A CN100548141C (en) | 2006-12-22 | 2006-12-22 | A kind of method of extracting rice protein and joint production of alcohol |
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| CNB2006101477687A CN100548141C (en) | 2006-12-22 | 2006-12-22 | A kind of method of extracting rice protein and joint production of alcohol |
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| CN100998367A true CN100998367A (en) | 2007-07-18 |
| CN100548141C CN100548141C (en) | 2009-10-14 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869173A (en) * | 2010-07-06 | 2010-10-27 | 长沙高新开发区湘博医药科技有限公司 | Method for extracting rice protein powder by utilizing enzyme-alkali method |
| CN102150737A (en) * | 2011-04-12 | 2011-08-17 | 江南大学 | Method for preparing high-purity rice protein and acquired products by using same |
| CN102370044A (en) * | 2011-09-30 | 2012-03-14 | 哈尔滨工业大学 | Extraction method of high-digestibility rice protein |
| CN102539364A (en) * | 2011-12-14 | 2012-07-04 | 贵州大学 | Method for detecting rice maturity |
| CN103555795A (en) * | 2013-10-23 | 2014-02-05 | 华中农业大学 | Method for co-producing high-purity rice starch and rice protein |
| CN107319098A (en) * | 2017-08-21 | 2017-11-07 | 河南恒宇食品科技股份有限公司 | wire-drawing protein and preparation method thereof |
-
2006
- 2006-12-22 CN CNB2006101477687A patent/CN100548141C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101869173A (en) * | 2010-07-06 | 2010-10-27 | 长沙高新开发区湘博医药科技有限公司 | Method for extracting rice protein powder by utilizing enzyme-alkali method |
| CN102150737A (en) * | 2011-04-12 | 2011-08-17 | 江南大学 | Method for preparing high-purity rice protein and acquired products by using same |
| CN102150737B (en) * | 2011-04-12 | 2012-10-03 | 江南大学 | Method for preparing high-purity rice protein and acquired products by using same |
| CN102370044A (en) * | 2011-09-30 | 2012-03-14 | 哈尔滨工业大学 | Extraction method of high-digestibility rice protein |
| CN102539364A (en) * | 2011-12-14 | 2012-07-04 | 贵州大学 | Method for detecting rice maturity |
| CN103555795A (en) * | 2013-10-23 | 2014-02-05 | 华中农业大学 | Method for co-producing high-purity rice starch and rice protein |
| CN103555795B (en) * | 2013-10-23 | 2016-02-03 | 华中农业大学 | A kind of method of co-producing high-purity Starch rice and rice protein |
| CN107319098A (en) * | 2017-08-21 | 2017-11-07 | 河南恒宇食品科技股份有限公司 | wire-drawing protein and preparation method thereof |
| CN107319098B (en) * | 2017-08-21 | 2021-03-12 | 河南恒宇食品科技股份有限公司 | Wiredrawing protein and preparation method thereof |
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| CN100548141C (en) | 2009-10-14 |
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