CN103039987A - Soybean antioxidant peptide microcapsule and preparation method thereof - Google Patents
Soybean antioxidant peptide microcapsule and preparation method thereof Download PDFInfo
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- CN103039987A CN103039987A CN2013100023140A CN201310002314A CN103039987A CN 103039987 A CN103039987 A CN 103039987A CN 2013100023140 A CN2013100023140 A CN 2013100023140A CN 201310002314 A CN201310002314 A CN 201310002314A CN 103039987 A CN103039987 A CN 103039987A
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- sodium alginate
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Abstract
The invention discloses a soybean antioxidant peptide microcapsule and a preparation method thereof, belongs to the technical field of deep processing of subsidiary agricultural products and comprehensive utilization of byproducts of the subsidiary agricultural products, and relates to a technology of preparing the soybean antioxidant peptide microcapsule by a sodium alginate-chitosan-sodium alginate two-step method. The preparation method comprises the technological processes of gelled pill preparation, film formation reaction, lamination film reaction and gelled pill solidification. The embedding rate of antioxidant peptide of the microcapsule can reach above 80%, and the microcapsule is an ideal product.
Description
Technical field:
The invention belongs to the technical field that agricultural byproducts intensive processing and byproduct comprehensive thereof utilize, relate to a kind of sodium alginate-chitose-sodium alginate two-step method of utilizing and prepare the soybean antioxidative peptide microcapsules, comprise the preparation of gelled pill, the generation of film formation reaction, the generation of overlay film reaction, the technical process of the curing of gelled pill, it can realize the embedding rate of its anti-oxidation peptide more than 80%.
Background technology:
Anti-oxidation peptide is a kind of functional peptide class health food with DEVELOPMENT PROSPECT, but reaction of degeneration (RD) very easily occurs under the conditions such as high temperature, humidity less stable, and this is greatly affected the application of functional peptide.And polypeptide class health food is easily by gastric juice digestive ferment hydrolytic inactivation, thereby reduces polypeptide drug release under one's belt and can improve its biologically active and utilization rate.Therefore, in order to prolong action time and the effect of taking rear Functional Polypeptides, and increase the stability of peptide, peptide is carried out the effect that microencapsulation can be played slowly-releasing and improve its stability.
The sodium alginate carrier is suitable for having bioactive protein-based food, and its source is abundant, with low cost, being up-and-coming slow-released carrier, be a kind of microcapsule wall material that extremely is fit to, and the source of shitosan is also very extensive, cheap, and can attract to form polyelectrolyte film by positive and negative charge with sodium alginate, thereby strengthen the stability of its micro-capsule, strengthen its slowly-releasing and put performance.Therefore, we adopt the sodium alginate-chitose-sodium alginate two-step method to prepare the soybean antioxidative peptide microcapsules.
Summary of the invention:
The technical problem of the required solution of application invention is: disclose a kind of method for preparing the soybean antioxidative peptide microcapsules
The technical scheme of application invention is
1. method for preparing the soybean antioxidative peptide microcapsules, it is characterized in that, utilize the sodium alginate-chitose-sodium alginate two-step method to prepare the soybean antioxidative peptide microcapsules, comprise the preparation of gelled pill, the generation of film formation reaction, the generation of overlay film reaction, the technical process of the curing of gelled pill, it can realize the embedding rate of its anti-oxidation peptide more than 80%.
1) preparation process of described gelled pill, be at first with sodium alginate in 40-50 ℃ distilled water, be stirred to fully dissolving, add again the powder of a certain amount of soybean antioxidative peptide, continue to stir ten minutes, it is uniformly dispersed in sodium alginate soln.Then with the Soybean Peptide sodium alginate soln that mixes joining in the calcium chloride solution drop by drop, rock beaker while drip, make that its bead disperses evenly.Then, with gelled pill from CaCl
2Suction filtration out washs gelled pill 3-5 time with distilled water in the solution.
2) described film formation reaction, to join with the gelled pill of distilled water wash clean in the 0.4-0.8% shitosan (mass fraction) that utilizes the dissolving of 1.0% hydrochloric acid (volume fraction), carry out film formation reaction 10-15min, will carry out again gelled pill washing 3-5 time of film formation reaction with distilled water.
3) generation of described overlay film reaction is that the gelled pill with the distilled water washes clean is joined in the sodium alginate soln of certain mass mark, and behind the reaction 5-10min, the gelled pill that will carry out the overlay film reaction with distilled water again washs 3-5 time.
4) curing of described gelled pill, that the gelled pill after the overlay film washing is joined crosslinking curing 2-6h in the formalin that volume fraction is 0.5-1.0%, the formaldehyde of distilled water flush away surface of microcapsule remnants, blot with the water of filter paper with surface of microcapsule, then in 4 ℃ refrigerator, preserve.
The preparation process of 2 microballoons according to claim 1, the mass fraction of its sodium alginate are 2%-4%, and the mass ratio of soybean antioxidative peptide and sodium alginate is 1: 3-1: 6, and the mass fraction of calcium chloride is 0.5%-2%.
3 film formation reactions according to claim 1, the mass fraction of its shitosan are 0.4-0.8%, and the reaction time is 10-15min.
4 overlay film reactions according to claim 1, the mass fraction of its sodium alginate is 0.2%-0.5%, the reaction time is 5-10min.
The curing of 5 microballoons according to claim 1, the mass fraction that it is characterized in that formaldehyde is 0.5-1%, be 2-6h. hardening time
Implementation
Embodiment 1:
Be that 3% sodium alginate soln dissolves in 40-50 ℃ distilled water with mass fraction, add the soybean antioxidative peptide of certain mass, its quality is 1/3 of sodium alginate, stirs ten minutes, and it is uniformly dispersed in sodium alginate soln.Then be in 1% the calcium chloride solution with the scattered solution mass fraction that joins drop by drop, rock beaker while drip, the bead size that makes its formation evenly and adhesion.Then, with gelled pill from CaCl
2Suction filtration out washs gelled pill 3-5 time with distilled water in the solution.To join with the gelled pill of distilled water wash clean in 0.4% shitosan (mass fraction) solution that utilizes the dissolving of 1.0% hydrochloric acid (volume fraction), carry out film formation reaction 10-15min, will carry out again gelled pill washing 3-5 time of film formation reaction with distilled water.To join with the gelled pill of distilled water wash clean mass fraction and be in 1% the sodium alginate soln, carry out overlay film reaction 5-10min, again with distilled water washing 3-5 time.It is crosslinking curing 4h in 1% the formalin that gelled pill after the overlay film washing is joined volume fraction, the formaldehyde of distilled water flush away surface of microcapsule remnants, blot with the water of filter paper with surface of microcapsule, prepare described soybean antioxidative peptide microcapsules, in 4 ℃ refrigerator, preserve.
Embodiment 2:
Be that 4% sodium alginate soln dissolves in 40-50 ℃ distilled water with mass fraction, add the soybean antioxidative peptide of certain mass, its quality is 1/4 of sodium alginate, stirs ten minutes, and it is uniformly dispersed in sodium alginate soln.Then be in 1% the calcium chloride solution with the scattered solution mass fraction that joins drop by drop, rock beaker while drip, the bead size that makes its formation evenly and adhesion.Then, with gelled pill from CaCl
2Suction filtration out washs gelled pill 3-5 time with distilled water in the solution.To join with the gelled pill of distilled water wash clean in 0.4% shitosan (mass fraction) solution that utilizes the dissolving of 1.0% hydrochloric acid (volume fraction), carry out film formation reaction 10-15min, will carry out again gelled pill washing 3-5 time of film formation reaction with distilled water.To join with the gelled pill of distilled water wash clean mass fraction and be in 1% the sodium alginate soln, carry out overlay film reaction 5-10min, again with distilled water washing 3-5 time.It is crosslinking curing 6h in 1% the formalin that gelled pill after the overlay film washing is joined volume fraction, the formaldehyde of distilled water flush away surface of microcapsule remnants, blot with the water of filter paper with surface of microcapsule, prepare described soybean antioxidative peptide microcapsules, in 4 ℃ refrigerator, preserve.
Claims (5)
1. method for preparing the soybean antioxidative peptide microcapsules, it is characterized in that, utilize the sodium alginate-chitose-sodium alginate two-step method to prepare the soybean antioxidative peptide microcapsules, comprise the preparation of gelled pill, the generation of film formation reaction, the generation of overlay film reaction, the technical process of the curing of gelled pill can realize the embedding rate of its anti-oxidation peptide more than 80%.
1) preparation process of described gelled pill, be at first with sodium alginate in 40-50 ℃ distilled water, be stirred to fully dissolving, add again the powder of a certain amount of soybean antioxidative peptide, continue to stir ten minutes, it is uniformly dispersed in sodium alginate soln.Then with the soybean antioxidative peptide sodium alginate soln that mixes joining in the calcium chloride solution drop by drop, rock beaker while drip, bead is disperseed uniformly.Then, with gelled pill from CaCl
2Suction filtration out washs gelled pill 3-5 time with distilled water in the solution.
2) described film formation reaction, to join with the gelled pill of distilled water wash clean in the 0.4-0.8% shitosan (mass fraction) that utilizes the dissolving of 1.0% hydrochloric acid (volume fraction), carry out film formation reaction 10-15min, will carry out again gelled pill washing 3-5 time of film formation reaction with distilled water.
3) generation of described overlay film reaction is that the gelled pill with the distilled water washes clean is joined in the sodium alginate soln of certain mass mark, behind the reaction 5-10min, with distilled water the gelled pill that overlay film reacts will occur again and wash 3-5 time.
4) curing of described gelled pill, that the gelled pill after overlay film reaction and the washing is joined volume fraction is crosslinking curing 2-6h in 1% the formalin, the formaldehyde of distilled water flush away surface of microcapsule remnants, blot with the water of filter paper with surface of microcapsule, then in 4 ℃ refrigerator, preserve.
2. the preparation process of gelled pill according to claim 1, the mass fraction of its sodium alginate is 2%-4%, the mass ratio of soybean antioxidative peptide and sodium alginate is 1: 3-1: 6, the mass fraction of calcium chloride is 0.5%-2%.
3. film formation reaction according to claim 1, the mass fraction of its shitosan is 0.4-0.8%, the reaction time is 10-15min.
4. overlay film reaction according to claim 1, the mass fraction of its sodium alginate is 0.2%-0.5%, reaction time 5-10min.
5. the curing of microballoon according to claim 1, the mass fraction that it is characterized in that formaldehyde is 0.5-1%, be 2-6h hardening time.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340408A (en) * | 2013-07-26 | 2013-10-09 | 湖南农业大学 | Rice antioxidant peptide microcapsule and preparation method thereof |
| CN103819541A (en) * | 2014-03-06 | 2014-05-28 | 福州大学 | Microalgae oxidation prevention polypeptide |
| CN104522291A (en) * | 2014-12-30 | 2015-04-22 | 东北农业大学 | Method for preparing egg white source ACE inhibitory peptide microcapsules |
| CN105919864A (en) * | 2016-05-31 | 2016-09-07 | 浙江大学 | Bamboo leaf flavonoid composite nano-particles with skin whitening effect and preparation method thereof |
| CN106723082A (en) * | 2016-12-29 | 2017-05-31 | 无限极（中国）有限公司 | A kind of active peptide microparticle formulation and preparation method thereof |
| CN108850778A (en) * | 2018-06-19 | 2018-11-23 | 佛山皖阳生物科技有限公司 | A kind of preparation method of long-acting stable type biomass antioxidant |
-
2013
- 2013-01-06 CN CN2013100023140A patent/CN103039987A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103340408A (en) * | 2013-07-26 | 2013-10-09 | 湖南农业大学 | Rice antioxidant peptide microcapsule and preparation method thereof |
| CN103340408B (en) * | 2013-07-26 | 2015-10-28 | 湖南农业大学 | A kind of rice antioxidant peptide microcapsule and preparation method thereof |
| CN103819541A (en) * | 2014-03-06 | 2014-05-28 | 福州大学 | Microalgae oxidation prevention polypeptide |
| CN104522291A (en) * | 2014-12-30 | 2015-04-22 | 东北农业大学 | Method for preparing egg white source ACE inhibitory peptide microcapsules |
| CN105919864A (en) * | 2016-05-31 | 2016-09-07 | 浙江大学 | Bamboo leaf flavonoid composite nano-particles with skin whitening effect and preparation method thereof |
| CN105919864B (en) * | 2016-05-31 | 2019-01-08 | 浙江大学 | Bamboo-leaves flavones composite nanoparticle with white-skinned face function and preparation method thereof |
| CN106723082A (en) * | 2016-12-29 | 2017-05-31 | 无限极（中国）有限公司 | A kind of active peptide microparticle formulation and preparation method thereof |
| CN106723082B (en) * | 2016-12-29 | 2020-09-18 | 无限极（中国）有限公司 | Active peptide particle preparation and preparation method thereof |
| CN108850778A (en) * | 2018-06-19 | 2018-11-23 | 佛山皖阳生物科技有限公司 | A kind of preparation method of long-acting stable type biomass antioxidant |
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Application publication date: 20130417 |