CN102212429B

CN102212429B - Preparation method of nitrogen source supplementing agent for producing beer

Info

Publication number: CN102212429B
Application number: CN2011100847917A
Authority: CN
Inventors: 王佐民; 吕伟民; 赵云财; 宋殿峰; 张劲松; 陈成; 孙东方; 李娜; 顾利文; 佟晓芳; 李强
Original assignee: HEILONGJIANG-PROV ACADEMY OF LIGHT INDUSTRY SCIENCES
Current assignee: HEILONGJIANG-PROV ACADEMY OF LIGHT INDUSTRY SCIENCES
Priority date: 2011-04-06
Filing date: 2011-04-06
Publication date: 2012-07-25
Anticipated expiration: 2031-04-06
Also published as: CN102212429A

Abstract

The invention provides a preparation method of a nitrogen source supplementing agent for producing beer and relates to a production method of an organic nitrogen source, in particular to a method for preparing a novel organic nitrogen source for producing beer from plant protein. The preparation method comprises the following steps of: adding water into raw materials until the concentration of the raw materials is 13 to 18 percent, wherein the raw materials comprise any one kind of or a mixture of two or three kinds of corn protein powder, soybean cake powder or peanut cake powder; performing moist heat sterilization; cooling to 40 to 45 DEG C; adding neutral protease to hydrolyze for 5 hours and then adjusting the pH value to 4.8 to 5.2; adding papain to hydrolyze for 8 hours; after the hydrolysis is finished, deactivating enzyme at high temperature; decoloring by using active carbon and then filtering; concentrating the filtrate; and performing spray drying to prepare solid powdered finished products.

Description

A kind of beer prodn is with the making method of nitrogenous source supplement

One, affiliated field

What the present invention relates to is a kind of working method of organic nitrogen source, specifically a kind of method of utilizing plant protein to make the novel organic nitrogen source that is used for beer prodn.

Two, technical background:

This project utilizes bionic method that vegetable-protein is changed into amino acid, in the beer fermentation industry, uses, and can effectively replenish the nitrogenous source in the beer fermentation substratum; Improve the AAN content in the wheat juice, reduce the Fructus Hordei Germinatus consumption, improve the consumption of beer prodn auxiliary material; Reduce cost, improve the beer fermentation degree, shorten fermentation period; Reduce diacetyl content, improve beer flavor, improve the non-biostability of beer.Low cost product of the present invention can fundamentally solve the requirement of nitrogenous source in the beer fermentation, need not add AMS enzyme and proteolytic enzyme, is widely used in the brewage industry.

Three, summary of the invention

Content of the present invention is achieved in that the mixture with any in Zein powder, soybean cake powder or the groundnut meal or two kinds or three kinds is a raw material; Add earlier water size mixing to material concentration be 13-18%; Moist heat sterilization 30 minutes under 121 ℃ of conditions cools to 40-45 ℃ then, adds the neutral protease of 20-25 unit by every gram raw material; Hydrolysis 5 hours; Be 4.8-5.2 with adjust pH then, add the hydrolysis 8 hours under 50 ℃ of-60 ℃ of conditions of 0.2% papoid (2000000u/g), carry out the high temperature enzyme that goes out after hydrolysis finishes; Then with 40-50 minute after-filtration of 50 ℃-60 ℃ decolourings of the gac of 0.3%-0.5% insulation, the reconcentration spraying drying to the 40-50Bx of will filtrating is processed the solid powdery finished product.

Four, embodiment

Mode one: take by weighing 150 gram Zein powders, add 850ml water, stir; Moist heat sterilization 30 minutes under 121 ℃ of conditions cools to 45 ℃ then, adds 0.075 gram neutral protease (50000u/g); Stir, insulation hydrolysis 5 hours is adjusted into 4.8-5.2 with the pH value then; Add 0.3 gram papoid (2000000u/g), temperature is risen to 60 ℃ continue insulation hydrolysis 8 hours, hydrolysis finishes 30 minutes enzymes that go out of moist heat sterilization under back 121 ℃ of conditions; Add 45 minutes after-filtration of 60 ℃ of decolourings of 4 gram gacs insulations then, the reconcentration spraying drying to the 45Bx of will filtrating is processed the solid powdery finished product.

Mode two: take by weighing 300 gram soybean cake powders, add 1800ml water, stir; Moist heat sterilization 30 minutes under 121 ℃ of conditions cools to 42 ℃ then, adds 0.075 gram neutral protease (50000u/g); Hydrolysis 5 hours is adjusted into 4.8-5.2 with the pH value then, adds 0.3 gram papoid (2000000u/g); Temperature is risen to 55 ℃ continue insulation hydrolysis 8 hours; Hydrolysis finishes 30 minutes enzymes that go out of moist heat sterilization under back 121 ℃ of conditions, adds 50 minutes after-filtration of 55 ℃ of decolourings of 8 gram gacs insulations then, and the reconcentration spraying drying to the 42Bx of will filtrating is processed the solid powdery finished product.

Claims

1. a beer prodn is characterized in that with the making method of nitrogenous source supplement: the mixture with any in Zein powder, soybean cake powder or the groundnut meal or two kinds or three kinds is a raw material, Xian Jiashui size mixing to material concentration be 13-18%; Moist heat sterilization 30 minutes under 121 ℃ of conditions cools to 40-45 ℃ then, adds the neutral protease of 20-25 unit by every gram raw material; Hydrolysis 5 hours; Be 4.8-5.2 with adjust pH then, add 0.2% papoid, the unit of activity of papoid is 2000000u/g; Hydrolysis is 8 hours under 50 ℃ of-60 ℃ of conditions; Hydrolysis finishes go out enzyme 30 minutes of back 121 ℃ of high temperature, and then with 40-50 minute after-filtration of 50 ℃-60 ℃ decolourings of gac insulation of 0.3%-0.5%, the reconcentration spraying drying to the 40-50Bx of will filtrating is processed the solid powdery finished product.