CN103038361B - 试剂盒和方法 - Google Patents

试剂盒和方法 Download PDF

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Publication number
CN103038361B
CN103038361B CN201180024021.1A CN201180024021A CN103038361B CN 103038361 B CN103038361 B CN 103038361B CN 201180024021 A CN201180024021 A CN 201180024021A CN 103038361 B CN103038361 B CN 103038361B
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CN
China
Prior art keywords
dna
deoxyribonucleoside
fluorescent moiety
kit
polymerase
Prior art date
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Active
Application number
CN201180024021.1A
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English (en)
Chinese (zh)
Other versions
CN103038361A (zh
Inventor
P·斯托尔汉德斯克
J·伦纳斯特兰德
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scissons Co
Original Assignee
Sisens
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91235—Phosphotransferases in general with a phosphate group as acceptor (2.7.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/91245—Nucleotidyltransferases (2.7.7)
    • G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)
    • G01N2333/9126—DNA-directed DNA polymerase (2.7.7.7)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CN201180024021.1A 2010-05-14 2011-05-13 试剂盒和方法 Active CN103038361B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE1050473 2010-05-14
SE1050473-6 2010-05-14
PCT/SE2011/050605 WO2011142719A1 (en) 2010-05-14 2011-05-13 Kit and method

Publications (2)

Publication Number Publication Date
CN103038361A CN103038361A (zh) 2013-04-10
CN103038361B true CN103038361B (zh) 2017-02-08

Family

ID=44641364

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201180024021.1A Active CN103038361B (zh) 2010-05-14 2011-05-13 试剂盒和方法

Country Status (6)

Country Link
US (2) US9429518B2 (cg-RX-API-DMAC7.html)
EP (1) EP2569441B2 (cg-RX-API-DMAC7.html)
JP (2) JP2013526273A (cg-RX-API-DMAC7.html)
CN (1) CN103038361B (cg-RX-API-DMAC7.html)
DK (1) DK2569441T4 (cg-RX-API-DMAC7.html)
WO (1) WO2011142719A1 (cg-RX-API-DMAC7.html)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2569441B2 (en) 2010-05-14 2017-09-13 cSens AB Kit and method for measuring the activity of deoxynucleoside kinase using dna-dependent dna polymerase and natural deoxynucleosides
CN110904187A (zh) * 2019-11-05 2020-03-24 翌圣生物科技（上海）有限公司 一种Taq酶活性测定方法
JP7875304B2 (ja) 2022-04-29 2026-06-17 ビオヴィカ インターナショナル アクティエ ボラーグ 免疫チェックポイント阻害剤の有効性のためのマーカーとしてのチミジンキナーゼ

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5273879A (en) * 1987-07-23 1993-12-28 Syntex (U.S.A.) Inc. Amplification method for polynucleotide assays
US5328827A (en) * 1987-08-27 1994-07-12 Institut National De La Recherche Agronomique - Inra Specific DNA molecular probes for Bos-type male genome
DE4020529A1 (de) 1989-09-12 1991-03-21 Roxana Vasiloiu Multifunktionelle(s) enzym(e) mit nucleosid-didesoxyribosyltransferase(n) und/oder nucleoside-desoxyribosyltransferasen(n) und/oder kinease- und/oder reduktase- und/oder desaminase- und/oder polymerase-aktivitaet
US5189019A (en) * 1990-04-23 1993-02-23 Merck & Co., Inc. Antistasin derived anticoagulant protein
US5321010A (en) * 1991-12-10 1994-06-14 Merck & Co., Inc. Proteins for inhibiting adhesion of platelets to collagen
WO1995012313A1 (en) 1993-11-05 1995-05-11 Pathogenesis Corporation Virus associated multiple sclerosis: treatments, prevention and diagnosis thereof
EP0745686A1 (en) * 1995-06-01 1996-12-04 Roche Diagnostics GmbH The use of DNA polymerase 3'-intrinsic editing activity
GB0022458D0 (en) * 2000-09-13 2000-11-01 Medical Res Council Directed evolution method
US7045319B2 (en) 2001-10-30 2006-05-16 Ribomed Biotechnologies, Inc. Molecular detection systems utilizing reiterative oligonucleotide synthesis
ATE352039T1 (de) 2002-07-27 2007-02-15 Diasorin Ab Verfahren zur bestimmung der aktivität von thymidinkinase-1 und dessen verwendung
US9505846B2 (en) 2005-01-28 2016-11-29 Life Technologies Corporation Multi-component inhibitors of nucleic acid polymerases
US8765378B2 (en) 2005-02-25 2014-07-01 Biovica International Ab Method and kit for determination of thymidine kinase activity and use thereof
WO2008024052A1 (en) 2006-08-24 2008-02-28 Rönnerbol International Ab A method and a kit for determination of an enzyme activity involved in metabolic production of a deoxynucleoside triphosphate and use thereof
GB0722449D0 (en) 2007-11-15 2007-12-27 Univ Abertay Dundee bIOASSAY
US20090197254A1 (en) * 2007-12-14 2009-08-06 Ming-Chou Lee Variant scorpion primers for nucleic acid amplification and detection
JP2009268381A (ja) 2008-05-01 2009-11-19 Univ Of Fukui 癌特異的酢酸代謝を標的とした癌バイオマーカー・検査方法・治療剤
GB0814591D0 (en) 2008-08-08 2008-09-17 Randox Lab Ltd Assay
WO2010036359A2 (en) * 2008-09-25 2010-04-01 Allelogic Biosciences Corporation Methods of polymerase activity assay
EP2569441B2 (en) 2010-05-14 2017-09-13 cSens AB Kit and method for measuring the activity of deoxynucleoside kinase using dna-dependent dna polymerase and natural deoxynucleosides

Also Published As

Publication number Publication date
CN103038361A (zh) 2013-04-10
JP2013526273A (ja) 2013-06-24
DK2569441T4 (en) 2017-12-18
WO2011142719A1 (en) 2011-11-17
US20130071846A1 (en) 2013-03-21
JP2017042169A (ja) 2017-03-02
EP2569441A4 (en) 2013-11-27
EP2569441B2 (en) 2017-09-13
JP6409233B2 (ja) 2018-10-24
EP2569441B1 (en) 2014-11-19
US10190149B2 (en) 2019-01-29
DK2569441T3 (en) 2015-01-05
US20170029867A1 (en) 2017-02-02
EP2569441A1 (en) 2013-03-20
US9429518B2 (en) 2016-08-30

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Effective date: 20150128

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